1319 results about "Biosynthesis" patented technology

The invention belongs to the technical field of gene engineering and enzyme engineering, and particularly relates to an ornithine decarboxylase mutant as well as a coding gene, an expression vector, a recombinant strain and application thereof. The ornithine decarboxylase mutant is obtained by mutating wild ornithine decarboxylase ODC10 of which the amino acid sequence is shown as SEQ ID NO.1, the catalytic activity of the obtained mutant is improved, and the biosynthesis efficiency of 1, 4-butanediamine is favorably improved. The recombinant strain for expressing the ornithine decarboxylase mutant constructed by the invention can efficiently synthesize 1, 4-butanediamine, and the molar conversion rate of ornithine is remarkably improved, so that the production benefit is improved, and the production cost is reduced.

The invention discloses construction and application of an engineering bacterium for co-expressing D-mannitol dehydrogenase and D-glucose dehydrogenase, and belongs to the technical field of genetic engineering. The technical problem to be solved by the invention is how to co-express D-mannitol dehydrogenase and D-glucose dehydrogenase through a biosynthetic pathway. In order to solve the technical problem, the invention provides recombinant escherichia coli. The recombinant escherichia coli is characterized by containing a D-mannitol dehydrogenase gene, a D-glucose dehydrogenase gene, a glucokinase gene, an acetyl coenzyme A synthetase gene, a D-lactose transporter gene, a D-glucose PTS permease-free gene and a pyruvate oxidase-free gene. And no regulator gene of galactose operon is contained. The invention establishes a preparation method for co-producing D-mannitol and sodium gluconate, which has guiding significance for the production of D-mannitol and sodium gluconate.

The invention discloses a biosynthesis system for high-yield 1, 6-hexamethylenediamine and application of the biosynthesis system, and belongs to the technical field of biology. According to the method, key genes which are excavated and optimized in a 1, 6-hexamethylenediamine biosynthetic pathway are co-expressed in a single cell module or a multi-cell module, and different enzyme elements expressed by the cell module are subjected to enzyme cascade reaction, so that 1, 6-hexamethylenediamine can be efficiently synthesized by taking caprolactam or 6-aminocaproic acid as a substrate; furthermore, after the carboxylic acid reductase MaCAR is mutated, the catalytic activity of the carboxylic acid reductase MaCAR can be remarkably improved, and the yield of the 1, 6-hexamethylenediamine is further improved; in addition, the biosynthesis system disclosed by the invention directly takes the cell module as a whole-cell catalyst, and an enzyme purification step is avoided, so that the biosynthesis system has the advantage of low cost.

The invention relates to the technical field of biology, in particular to a beta-1, 4-galactosyl transferase mutant and a method for biologically synthesizing lactose-N-neotetraose by using the beta-1, 4-galactosyl transferase mutant. The invention discloses a protein, which is a protein HpgalT-186 or a protein HpgalT-146-186, a protein HpgalT-146 and a protein HpgalT-146. The protein HpgalT-186 is a protein obtained by mutating an amino acid residue at the 186th site of a wild type HpgalT; the protein HpgalT-146-186 is a protein obtained by carrying out mutation on an amino acid residue at the 186 site and an amino acid residue at the 146 site of wild type HpgalT. On the basis, a series of escherichia coli is constructed and optimized, the yield of the LNnT synthesized through shake flask fermentation of the finally obtained strain reaches 1.73-4.1 g / L, the highest yield in a 5L fermentation tank reaches 20-45.2 g / L, and efficient synthesis of the LNnT is achieved.

The invention provides recombinant yeast for producing bakuchiol as well as a construction method and application of the recombinant yeast. Wherein the recombinant yeast is Yarrowia lipolytica containing isopentenyl transferase, and the recombinant yeast is a Yarrowia lipolytica containing isopentenyl transferase. Wherein a gene for coding the isopentenyl transferase is derived from Psoralea coryllifelia Linn (Psoralea coryllifelia Linn), and the isopentenyl transferase is used for coding the isopentenyl transferase. By utilizing the recombinant yeast provided by the invention, the bakuchiol with high yield can be efficiently and quickly prepared through a biosynthesis method, and a technical support is provided for subsequent industrialization of the bakuchiol.

The invention discloses a recombinant neurospora crassicola strain for producing astaxanthin as well as a construction method and application of the recombinant neurospora crassicola strain. The recombinant neurospora crassa contains key enzymes beta-carotene ketolase and beta-carotene hydroxylase in a heterologous astaxanthin synthetic pathway, and through pathway expression of a targeted subcellular compartment, crosstalk among metabolites is effectively prevented, metabolic flux of a biosynthetic pathway is coordinated, and the activity of the neurospora crassa is improved. The effective contact between the heterologous protein and the substrate in the astaxanthin synthetic route is promoted, the sporulation capability of the heterologous protein is improved, the astaxanthin production and storage capability of the neurospora crassiforme is effectively improved, and the method has relatively high popularization and application values.

The invention belongs to the technical field of biology, and particularly relates to a difunctional gene cluster biosynthesized by phenol amine and salicylic acid and application of the difunctional gene cluster. The gene cluster comprises five genes, the five genes are S1EPS1, S1PBS3, S1CoAOMT2, S1CoOOMT3 and S1CoAOMT4, the nucleotide sequence of the S1EPS1 gene is as shown in SEQ ID No.1, the nucleotide sequence of the S1PBS3 gene is as shown in SEQ ID No.3, the nucleotide sequence of the S1CoAOMT2 gene is as shown in SEQ ID No.5, the nucleotide sequence of the S1CoOOMT3 gene is as shown in SEQ ID No.7, and the nucleotide sequence of the S1CoAOMT4 gene is as shown in SEQ ID No.9. The invention further discloses a preparation method of the gene cluster. The overexpressed gene cluster component SlEPS1 gene can significantly enhance accumulation of spermidine derivatives and salicylic acid of tomato plants so as to enhance the resistance to Pst DC3000, lays a foundation for genetic improvement in the aspect of tomato disease resistance by using the gene cluster, and also provides technical reference for excavation of related metabolic gene clusters in other crops and in the aspect of resistance breeding.

The invention discloses a large-model-driven intelligent biosynthesis system and method, and the system employs a centralized multi-large-model intelligent agent architecture, and executes a task through a modularized intelligent agent. The system is composed of a man-machine cooperation interaction module, a global task management module, a general information retrieval module, a reverse path planning module, a protease modification module, a metabolic pathway optimization module, a wet experiment tool execution module and a code execution module. Each module integrates different tools and data sources for specific task fields. By means of the system, complex biosynthesis experiment design, planning and execution can be completed autonomously, functional modules and wet experiment feedback can be dynamically adapted, and the flexibility of experiment planning is ensured.

The present invention relates to a method for producing a transgenic plant having an increased saponin content. It is confirmed that a nucleotide having a base sequence represented by SEQ ID NO: 1 or 2 derived from ginseng Panax ginseng increases ginsenoside accumulation by regulating the expression of genes involved in ginsenoside biosynthesis. Therefore, the method can be advantageously applied to the production of transgenic plants with increased saponin content.

The invention discloses an indole-benzothiadiazole derivative as well as a preparation method and application thereof. The general structural formula of the indole-benzothiadiazole derivative provided by the invention is shown in the specification, wherein R is selected from one of methyl piperazinyl, diethylamino, azetidinyl and pyrrolidinyl. The derivative can be specifically combined and fluorescently labeled with mitochondrial RNA in cells, and has good selectivity, high fluorescence signal-to-noise ratio and excellent anti-interference capability in a complex biological system. Meanwhile, the indole-benzothiadiazole derivative provided by the invention is simple in synthetic route, easily available in raw materials, good in stability and convenient to store and use, can be specifically combined with RNA and generate strong fluorescence response, is suitable for real-time detection and imaging of in-vitro and in-vivo mitochondrial RNA, and is an efficient mitochondrial RNA micromolecular fluorescent probe.

The invention provides a biosynthesis inhibitor of migratory locust aggregation pheromone 4-vinyl anisole and application of the biosynthesis inhibitor. The biosynthesis inhibitor of migratory locust aggregation pheromone 4-vinylanisole reduces the activity of 4-vinylphenol methyltransferase, and the 4-vinylphenol methyltransferase contains an amino acid sequence selected from the following sequences: (a) a sequence as shown in any one of SEQ ID No.1 and SEQ ID No.3; and (b) a sequence which is obtained by deleting, inserting and / or substituting one or more amino acid residues in the sequence shown in any one of SEQ ID No.1 and SEQ ID No.3 and has the activity of catalyzing generation of migratory locust aggregation pheromone 4-vinyl anisole. The inhibitor can be applied to biological prevention and control of migratory locusts, can keep locusts in a harmless scattered state instead of killing the locusts, provides a new method for biological prevention and control of migratory locusts, and has a wide application prospect.

The invention relates to the technical field of biology, in particular to a method for synthesizing S-sulfo-L-cysteine through multi-enzyme cascade catalysis. The invention provides a new route for biosynthesis of S-sulfo-L-cysteine, and a test result shows that serine, AMP, sodium hexametaphosphate and sodium thiosulfate are used as substrates, and the S-sulfo-L-cysteine is synthesized by a one-step method. A crude enzyme liquid which contains serine kinase, polyphosphoric acid-AMP phosphotransferase and phosphoserine dependent type S-sulfo-L-cysteine synthetase in which the background of tryptophanase and glutaredoxin cells is knocked out is used for synthesizing the S-sulfo-L-cysteine, the steps are simple, the product yield is high, the cost is low, and the method has a relatively strong industrial application prospect.

The invention discloses a poplar PdbHLH57 gene as well as an expression protein and application thereof, and relates to the technical field of plant genetic engineering. The nucleotide sequence of the poplar PdbHLH57 gene disclosed by the invention is as shown in SEQ ID NO.1, and the amino acid sequence of the expression protein of the poplar PdbHLH57 gene is as shown in SEQ ID NO.2. The method comprises the following steps: constructing an expression vector of a poplar PdbHLH57 gene, and transforming the expression vector into a poplar leaf; and cultivating and screening to obtain the transgenic poplar with red leaves. Results show that compared with wild poplar, the PdbHLH57 gene for promoting poplar anthocyanin synthesis has the advantages that transgenic poplar leaves become red, and the anthocyanin content in transgenic plant leaves is remarkably increased; the expression level of anthocyanin biosynthesis related genes is remarkably increased, and further, it is shown that the PdbHLH57 gene plays an important role in poplar anthocyanin biosynthesis.

The invention provides a method for biosynthesizing human structural material type VI collagen. The collagen comprises an amino acid sequence as shown in SEQ ID NO: 2 or a variant amino acid sequence obtained by mutating the amino acid sequence, and the variant amino acid sequence retains the function of the amino acid sequence as shown in SEQ ID NO: 2. The collagen provided by the invention can promote cell adhesion and has a triple helix structure.

The invention provides a patchouli transcription factor PcIDD2 and application of the patchouli transcription factor PcIDD2 in forward regulation of biosynthesis of patchouli ketone. The patchouli transcription factor PcIDD2 has an amino acid sequence as shown in SEQ ID NO.1; the transcription factor is a potential transcription factor with a function of promoting biosynthesis of the patchouli ketone, and has important significance in promoting efficient synthesis of the patchouli ketone.

The invention belongs to the field of biosynthesis, and in particular relates to LBD (Levandanenediol Pyrophosphate) synthase and engineered yeast for producing sclareol. In order to solve the problem that in the prior art, the sclareol biosynthesis yield is generally low, LSLPPs mutant containing at least one mutation of D730L, D374L, N674L or G379M is obtained by performing mutation and optimization on LSLPPs pyrophosphate synthase, and the mutant is used for sclareol synthesis. Meanwhile, in order to solve the problem of genetic instability caused by free plasmid expression of part of saccharomyces cerevisiae strains, a sclareol synthesis pathway is constructed in a yeast genome, and meanwhile, an acetyl coenzyme A synthesis pathway, an MVA pathway and a sclareol synthesis pathway are enhanced; chassis bacteria for synthesizing sclareol are modified in a manner of knocking out part of transcription inhibition factors, and the yield of a 5L fermentation tank reaches 26.11 g / L and is greatly improved compared with the prior art.

The invention discloses a flavonoid glycoside glycosyltransferase LbUGT71BX1 in laportea bulbifera as well as the coding gene and the use thereof. The amino acid sequence of the flavonoid glycoside glycosyltransferase LbUGT71BX1 is shown as SEQ ID NO: 2. The nucleotide sequence of the coding gene of the flavonoid glycoside glycosyltransferase LbUGT71BX1 is shown as SEQ ID NO: 1. According to the invention, on the basis of relevant results of the second-generation transcriptome and the third-generation full-length transcriptome sequencing of the laportea bulbifera, the last-step key enzyme LbUGT71BX1 for the synthesis of the flavonoid glycoside in the laportea bulbifera is screened and identified by using a reverse genetics method, filling the terminal blank of the biosynthesis pathway of the flavonoid glycoside in the laportea bulbifera.

The invention discloses application of an apple MdMYS1 gene in regulating and controlling the wax content of plant fruits and leaves, and belongs to the technical field of plant genetic engineering. According to the invention, the MdMYS1 gene with high expression quantity in apple varieties with more waxiness is separated, and the nucleotide sequence of the MdMYS1 gene is shown as SEQ ID NO. 1. Through subcellular localization, the transcription factor expressed by the MdMYS1 gene is found to be localized on a cell nucleus. Experimental results show that overexpression of the MdMYS1 gene can significantly increase the wax content of the apple fruits and leaves by promoting biosynthesis of wax, which indicates that the MdMYS1 gene plays a key role in regulation and control of the wax content of the apple fruits and leaves. The invention provides an efficient and rapid way for apple breeding, provides a gene material for improving apple quality, and has a wide application prospect in improving economic benefits and ecological benefits of apple planting.

The invention belongs to the field of biosynthesis, and particularly relates to sclareol synthase and engineered yeast for producing sclareol. In order to solve the problem that in the prior art, sclareol biosynthesis yield is generally low, sclareol synthase is mutated and optimized, an SsScs mutant containing at least one mutation of Y491F, G307L or N269F is obtained, and the mutant is used for sclareol synthesis. Meanwhile, in order to solve the problem of genetic instability caused by free plasmid expression of part of saccharomyces cerevisiae strains, a sclareol synthesis pathway is constructed in a yeast genome, and meanwhile, an acetyl coenzyme A synthesis pathway, an MVA pathway and a sclareol synthesis pathway are enhanced; chassis bacteria for synthesizing sclareol are modified in a manner of knocking out part of transcription inhibition factors, and the yield of a 5L fermentation tank reaches 21.13 g / L and is greatly improved compared with the prior art.

The present invention discloses a yeast engineering bacterium containing a (-)-α-bisabolol synthase mutant and its application. By designing (-)-α-bisabolol synthase, the present invention obtains a mutant with high expression level, co-expresses the mutant with ERG20, highly expresses the key rate-limiting enzymes in the metabolic pathway, deletes the promoter to reduce the side reaction pathway, and adopts a multi-copy strategy to improve the metabolic flux in the (-)-α-bisabolol biosynthesis pathway. The shake-flask fermentation yield of the yeast engineering bacterium constructed by the present invention is close to 70 mg / L, which is about 35 times higher than that of the starting strain, and has broad application prospects in the biological fermentation production of (-)-α-bisabolol.

The invention relates to a biosynthesis method of cosmetic tripeptide GHK (glycine-histidine-lysine), which is characterized in that a GHK oligopeptide is prepared by carrying out microbial fermentation synthesis and trypsin specific hydrolysis on artificially designed GHK multiple artificial sequences. And on the basis of the original sequence, the GHK yield is improved by adding a specific interval sequence. Compared with a traditional chemical synthesis method, the GHK oligopeptide biosynthesis method has the advantages that raw materials are cheap and simple, steps are easy to implement, and the GHK oligopeptide biosynthesis method is environmentally friendly and beneficial to improving the application level of GHK tripeptide in the field of beauty makeup.

The invention relates to a recombinant thallus, a construction method of the recombinant thallus and application of the recombinant thallus in production of xanthoxylin WGX-50, and relates to the technical field of bioengineering. A recombinant plasmid containing a phenylalanine ammonialyase gene PAL with a nucleotide sequence as shown in SEQ ID NO.2 and a cinnamic acid coenzyme A ligase gene CNL with a nucleotide sequence as shown in SEQ ID NO.1 and a recombinant plasmid containing a tyramine N-hydroxycinnamoyl transferase gene THT with a nucleotide sequence as shown in SEQ ID NO.3 are introduced into the recombinant plasmid. A phenylalanine ammonialyase gene PAL, a cinnamic acid coenzyme A ligase gene CNL and a tyramine N-hydroxycinnamoyl transferase gene THT are overexpressed in escherichia coli, so that a biological synthesis path of xanthoxylin WGX-50 is formed by taking 3, 4-dioxomethyl phenylethylamine as a substrate for the first time, a foundation is laid for large-scale industrial production, and the method has a wide application prospect. The method has important economic value and social benefit.

The invention discloses interference engineering bacteria for blocking early infection of animal parasitic nematodes and application, and belongs to the field of animal parasitic disease prevention and treatment. According to the interference engineering bacteria disclosed by the invention, the HCON00038080 (cyp-22a1) gene of haemonchus contortus is specifically silenced by expressing double-stranded RNA (dsRNA), so that the biosynthesis of endogenous bile acid-like steroid hormone of the haemonchus contortus is inhibited, the activation of infectious larvae is influenced, and the infection establishment process of the infectious larvae is blocked. The invention can be used for preventing early infection of haemonchus disease of ruminants such as cattle, sheep, camels and the like and the phenomenon of spring / perinatal high tide, has higher application value for prevention and control of haemonchus disease, and has reference value for prevention and control of parasitic nematode disease of other animals.

The invention discloses a novel biosynthesis path for producing 3 '-phosphoadenosine-5'-phosphosulfuric acid and application, and belongs to the technical field of bioengineering. According to the invention, through recombinant expression and purification of ribose kinase, ribose phosphate pyrophosphate kinase, ribose phosphate transferase, adenine thiotransferase, adenosine phosphate sulfate kinase and polyphosphate kinase 2, a six-enzyme cascade reaction taking ribose and adenosine as substrates in vitro is constructed, the molar conversion rate reaches 22.85%, and the escherichia coli and bacillus subtilis PAPS cell factory can be used for preparing the recombinant ribose phosphate pyrophosphate kinase, the ribose phosphate transferase, the adenine thiotransferase, the adenosine phosphate sulfate kinase and the polyphosphate kinase 2. The yield of PAPS in a 5-L fermentation tank reaches 7.60 g / L and 5.03 g / L respectively. The PAPS synthesis route provided by the invention provides an extensible, economical and efficient solution for the production of sulfated compounds.

The invention discloses a gene for regulating and controlling taro flavone biosynthesis and application thereof, and belongs to the technical field of biology. The gene comprises a CeHCT1 gene and a CeHCT2 gene; the nucleotide sequence of the CeHCT1 gene is as shown in SEQ ID NO. 9; and the nucleotide sequence of the CeHCT2 gene is as shown in SEQ ID NO. 10. The invention also provides application of the gene in regulation and control of taro flavone content and improvement of plant nutritional quality. The CeHCT1 and / or CeHCT2 genes of the main cultivated variety Xinhua Longxiang taro are / is subjected to multi-target knockout through a gene editing technology, the flavonoid content in the taro can be effectively increased, the nutritional quality of the taro is efficiently enhanced, and the method has a great significance in accelerating development and utilization of taro genes and has a great application prospect. The method for improving the nutritional quality of the taros by applying a biological enhancement technology has important theoretical and practical significance.

The invention focuses on the fields of biotechnology and sanitary disinfection, and successfully separates five brand-new small molecule peptides (small molecule peptides K, L, M, N and O) from herba violae and spica prunellae. According to the innovative extraction method from raw material pretreatment, mixed extraction to multi-step purification, a disinfection action mechanism of destroying pathogen cell membranes, interfering energy metabolism and inhibiting biosynthesis by small molecular peptides is defined. The small molecule peptides show the advantages of efficient disinfection activity, high safety, good stability and difficult generation of drug resistance. On the basis, a series of products such as hygienic disinfection spray, wet tissues and hand sanitizer are developed, the market application prospect is wide, remarkable economic and social benefits can be brought, and a brand-new and high-quality solution is provided for the field of hygienic disinfection.

The invention relates to an ardisia crenata carbon glycosyl transferase AcCGT64 gene and application of the ardisia crenata carbon glycosyl transferase AcCGT64 gene in preparation of bergenin, and belongs to the technical field of biology. The nucleotide sequence of the ardisia crenata carbon glycosyl transferase AcCGT64 gene is as shown in SEQ ID NO. 1, and the total length of the sequence is 1359 bp; the amino acid sequence of the encoded protein is as shown in SEQ ID NO.2, and 451 amino acid residues are encoded. The ardisia crenata carbon glycosyl transferase AcCGT64 gene disclosed by the invention can be used as a biosynthesis regulation gene of bergenin, is applied to preparation of bergenin, and is remarkable in application prospect and easy to popularize and apply.

The invention relates to a giantleaf ardisia rhizome oxygen methyltransferase AgOMT03 gene and application thereof in preparation of bergenin, and belongs to the technical field of biology. The nucleotide sequence of the giantleaf ardisia rhizome oxygen methyltransferase AgOMT03 gene is as shown in SEQ ID NO.1, and the total length of the sequence is 1095bp; the amino acid sequence of the encoded protein is as shown in SEQ ID NO.2, and 364 amino acid residues are encoded. The giantleaf ardisia rhizome oxygen methyltransferase AgOMT03 gene disclosed by the invention can be used as a biosynthesis regulation gene of bergenin, is applied to preparation of bergenin, and is remarkable in application prospect and easy to popularize and apply.

The invention is suitable for the technical field of gene engineering, and provides a soybean MYB transcription factor gene and application thereof in increasing the content of isoflavone. The soybean MYB transcription factor gene is GmMYB3a, and the nucleotide sequence of the soybean MYB transcription factor gene is as shown in SEQ ID NO. 1. According to the invention, a pTF101-GmMYB3a overexpression vector and an RNAi-GmMYB3a interference vector are successfully constructed, and the constructed vectors are transferred into a receptor soybean variety Williams 82. Experimental results show that the soybean strain with overexpression of the GmMYB3a gene shows higher isoflavone content, while the soybean strain with interference of the GmMYB3a gene shows lower isoflavone content, and the positive regulation effect of the GmMYB3a in the isoflavone biosynthesis process is proved. The invention not only provides an innovative molecular breeding strategy for cultivating soybean strains with high isoflavone content, but also has remarkable application potential and market prospect.

The invention discloses a method for efficiently synthesizing epiinositol by catalyzing muscle inositol through double-enzyme cascade, and belongs to the field of biological catalysis engineering. According to the invention, scyllo-inositol dehydrogenase is mutated to obtain a beneficial mutant C261R, and the beneficial mutant C261R is coupled with heat-resistant myo-inositol dehydrogenase to efficiently catalyze and synthesize the epiinositol, and belongs to the field of biological catalysis engineering. The new way for synthesizing the epiinositol is completely different from reported synthesis methods of scyllo-inositol and D-chiro-inositol, and comprises the following steps of: firstly dehydrogenating the C4 position of the muscle inositol to form an intermediate product ketone, and then hydrogenating to realize chiral inversion of hydrogen at the C4 position, so as to obtain the final product epiinositol. The invention provides a brand new way for biocatalytically synthesizing the epiinositol, the yield of the obtained epiinositol is 1.39 g / L, the conversion rate is 38.7%, the yield and the conversion rate are the highest level which is publically reported at present, the biosynthesis process is simple to operate, low in cost, green and environment-friendly, and a solid research foundation is laid for industrial production of the epiinositol.