522 results about "Biosynthesis" patented technology

The invention discloses a biosynthesis system for high-yield 1, 6-hexamethylenediamine and application of the biosynthesis system, and belongs to the technical field of biology. According to the method, key genes which are excavated and optimized in a 1, 6-hexamethylenediamine biosynthetic pathway are co-expressed in a single cell module or a multi-cell module, and different enzyme elements expressed by the cell module are subjected to enzyme cascade reaction, so that 1, 6-hexamethylenediamine can be efficiently synthesized by taking caprolactam or 6-aminocaproic acid as a substrate; furthermore, after the carboxylic acid reductase MaCAR is mutated, the catalytic activity of the carboxylic acid reductase MaCAR can be remarkably improved, and the yield of the 1, 6-hexamethylenediamine is further improved; in addition, the biosynthesis system disclosed by the invention directly takes the cell module as a whole-cell catalyst, and an enzyme purification step is avoided, so that the biosynthesis system has the advantage of low cost.

The invention discloses an indole-benzothiadiazole derivative as well as a preparation method and application thereof. The general structural formula of the indole-benzothiadiazole derivative provided by the invention is shown in the specification, wherein R is selected from one of methyl piperazinyl, diethylamino, azetidinyl and pyrrolidinyl. The derivative can be specifically combined and fluorescently labeled with mitochondrial RNA in cells, and has good selectivity, high fluorescence signal-to-noise ratio and excellent anti-interference capability in a complex biological system. Meanwhile, the indole-benzothiadiazole derivative provided by the invention is simple in synthetic route, easily available in raw materials, good in stability and convenient to store and use, can be specifically combined with RNA and generate strong fluorescence response, is suitable for real-time detection and imaging of in-vitro and in-vivo mitochondrial RNA, and is an efficient mitochondrial RNA micromolecular fluorescent probe.

The invention belongs to the field of biosynthesis, and in particular relates to LBD (Levandanenediol Pyrophosphate) synthase and engineered yeast for producing sclareol. In order to solve the problem that in the prior art, the sclareol biosynthesis yield is generally low, LSLPPs mutant containing at least one mutation of D730L, D374L, N674L or G379M is obtained by performing mutation and optimization on LSLPPs pyrophosphate synthase, and the mutant is used for sclareol synthesis. Meanwhile, in order to solve the problem of genetic instability caused by free plasmid expression of part of saccharomyces cerevisiae strains, a sclareol synthesis pathway is constructed in a yeast genome, and meanwhile, an acetyl coenzyme A synthesis pathway, an MVA pathway and a sclareol synthesis pathway are enhanced; chassis bacteria for synthesizing sclareol are modified in a manner of knocking out part of transcription inhibition factors, and the yield of a 5L fermentation tank reaches 26.11 g / L and is greatly improved compared with the prior art.

The invention discloses application of an apple MdMYS1 gene in regulating and controlling the wax content of plant fruits and leaves, and belongs to the technical field of plant genetic engineering. According to the invention, the MdMYS1 gene with high expression quantity in apple varieties with more waxiness is separated, and the nucleotide sequence of the MdMYS1 gene is shown as SEQ ID NO. 1. Through subcellular localization, the transcription factor expressed by the MdMYS1 gene is found to be localized on a cell nucleus. Experimental results show that overexpression of the MdMYS1 gene can significantly increase the wax content of the apple fruits and leaves by promoting biosynthesis of wax, which indicates that the MdMYS1 gene plays a key role in regulation and control of the wax content of the apple fruits and leaves. The invention provides an efficient and rapid way for apple breeding, provides a gene material for improving apple quality, and has a wide application prospect in improving economic benefits and ecological benefits of apple planting.

The invention belongs to the field of biosynthesis, and particularly relates to sclareol synthase and engineered yeast for producing sclareol. In order to solve the problem that in the prior art, sclareol biosynthesis yield is generally low, sclareol synthase is mutated and optimized, an SsScs mutant containing at least one mutation of Y491F, G307L or N269F is obtained, and the mutant is used for sclareol synthesis. Meanwhile, in order to solve the problem of genetic instability caused by free plasmid expression of part of saccharomyces cerevisiae strains, a sclareol synthesis pathway is constructed in a yeast genome, and meanwhile, an acetyl coenzyme A synthesis pathway, an MVA pathway and a sclareol synthesis pathway are enhanced; chassis bacteria for synthesizing sclareol are modified in a manner of knocking out part of transcription inhibition factors, and the yield of a 5L fermentation tank reaches 21.13 g / L and is greatly improved compared with the prior art.

The invention belongs to the technical field of genetic engineering breeding, and particularly relates to application and a method of a transcription factor CaBTF3 for regulating capsorubin synthesis. According to the capsicum transcription factor CaBTF3 and the coding gene thereof provided by the invention, the biosynthesis of capsorubin can be influenced by adjusting the expression level of a key enzyme gene in a capsorubin synthesis route and regulating and controlling the development and quantity of fruit chloroplast, the expression of the CaBTF3 gene in capsicum fruits is inhibited, the accumulation amount of capsorubin in the capsicum fruits can be reduced, and the yield of capsorubin is increased. The color of the pepper fruit is further influenced. By enhancing the expression level of the CaBTF3 gene, the content of capsorubin in capsicum fruits can be increased, the gene is applied to plant genetic engineering breeding, a new effective way is provided for creating capsicum varieties with high capsorubin content, and the gene plays an important role in improving the economic benefits of the capsicum industry and popularizing and applying the capsorubin.

The invention relates to a diacylglycerol acyltransferase capable of synthesizing triglyceride as well as a coding gene and application of the diacylglycerol acyltransferase, and belongs to the field of enzymology, the nucleotide sequence of the gene for coding the diacylglycerol acyltransferase is as shown in SEQ ID NO.1, and the amino acid sequence of the diacylglycerol acyltransferase is as shown in SEQ ID NO.2. The invention also provides a recombinant plasmid and a recombinant engineering bacterium containing the gene of the enzyme. Diglyceride and an acyl donor are used as substrates, and triglyceride is catalytically synthesized through an acyl transfer reaction by using the enzyme. The invention provides an efficient tool for realizing enzymatic biosynthesis and large-scale production of natural triglyceride.

The invention belongs to the technical field of synthetic biology, and particularly relates to recombinant escherichia coli for efficiently producing O-acetyl-L-homoserine as well as a construction method and application of the recombinant escherichia coli. According to the invention, CRISPR-Cas9 gene editing is combined with metabolic modification, so as to precisely integrate OAH biosynthesis key nodes: introducing and overexpressing a mutant metX gene at a genome pseudogene site to enhance the expression of homoserine acetyltransferase, knocking out byproducts such as poxB and mgsA to synthesize genes, and carrying out high-yield synthesis of OAH. Corynebacterium glutamicum thrA (anti-feedback inhibition), aspB and bacillus subtilis acsA-acuA genes (construction of an acetic acid switch) are introduced, gene expression of gltA, tpiA and the like is optimized to increase supply of precursors such as acetyl CoA and the like, and finally a stable strain without plasmids, antibiotics and inducers is obtained. The production cost is effectively reduced, and the method has the advantage of high genetic stability and shows a good application prospect in OAH industrial production.

The invention belongs to the technical field of biosynthesis, and particularly relates to a method for regulating and controlling stevioside production through a UDPG synthesis route. According to the invention, a high-efficiency stevioside biosynthesis system is constructed through systematic engineering modification: in the aspect of chassis cell optimization, transcription factors (AtWRKY18, AtWRKY40 and AtMYC2) for regulating and controlling MEP pathway and key genes (dxs and / or dxr) of the MEP pathway are over-expressed, so that supply of terpene precursors is enhanced; and a heterologous MVA pathway (over-expression T7-atoB-mvaS-mvaE-mvK / mvKmut-pmK-mvaD-idi) with a modular design is introduced, so that sufficient supply of the terpenoid precursor is realized. Meanwhile, the intracellular concentration of the UDPG is increased by systematically enhancing a UDPG synthesis pathway (knocking out ugd, glgc, ushA, agp, deoA, tdk, udp, or thrA, and overexpressing galU, pgm, pyrE, pyrH, ugpA, or prs), so that the production capacity of the stevioside is remarkably improved.

The invention belongs to the technical field of biosynthesis, and particularly relates to a method for regulating and controlling stevioside production by improving the utilization rate of a carbon source. According to the invention, a high-efficiency stevioside biosynthesis system is constructed through systematic engineering modification: in the aspect of chassis cell optimization, transcription factors (AtWRKY18, AtWRKY40 and AtMYC2) for regulating and controlling MEP pathway and key genes (dxs and / or dxr) of the MEP pathway are over-expressed, so that supply of terpene precursors is enhanced; and a heterologous MVA pathway (over-expression T7-atoB-mvaS-mvaE-mvK / mvKmut-pmK-mvaD-idi) with a modular design is introduced, so that sufficient supply of the terpenoid precursor is realized. Meanwhile, by knocking out related genes and bypass metabolism genes (mgsA, iclR, menA or adhE) synthesized by organic acid, a glucose transport and utilization system (fnr, fadR, ppc, zwf or pgi is knocked out, and ACS, fbaA, tpiA, glf, galP or ptsG is overexpressed, so that the utilization rate of a carbon source is further improved, and the production capacity of stevioside is remarkably improved.

The invention provides an engineering bacterium and a method for synthesizing sclarediol. Wherein the engineering bacterium comprises a sclareone synthase gene and a Bayer-Villiger monooxygenase gene, and the sclareone synthase comprises: (a1) a protein having any sequence in SEQ ID NOs: 1-25, or (a2) a protein having any sequence in SEQ ID NOs: 2-25, or (a3) a protein having any sequence in SEQ ID NOs: 2-25; (a2) protein which has more than 70% of homology with the amino acid sequence limited in (a1) and has sclareone synthase activity; the Bayer-Villiger monooxygenase comprises: (b1) a protein having any one sequence in SEQ ID NOs: 26-50, or (b2) a protein having any one sequence in SEQ ID NOs: 26-50; (b2) is protein which has more than 70% of homology with the amino acid sequence limited in (b1) and has Bayer-Villiger monooxygenase activity. The method can solve the problem of difficulty in efficient biosynthesis of sclarediol in the prior art, and is suitable for the field of synthetic biology.

The invention relates to the technical field of synthesis of natural dyes by a biological method, in particular to an antibiotic-demand-free ornamental blue high-yield strain as well as construction and application of the antibiotic-demand-free ornamental blue high-yield strain. Corynebacterium glutamicum is used as an initial strain, a tricarboxylic acid circulating gene is regulated and interfered, and the copy number of exogenous blue pigment synthetase BpsA in a genome is adjusted, so that the ornamental blue high-yield strain is obtained. The strain does not need to use antibiotics in the fermentation process, the yield is stable, the product production capacity equivalent to the level when antibiotics are used can still be achieved through fermentation under the antibiotic-free condition, and the growth performance of the strain is kept. The method for producing the ornamental blue by using the strain provided by the invention ensures that the ornamental blue product is natural and green, also improves the efficient production capacity of the strain, solves the contradiction between green environmental protection and stable high yield of the ornamental blue dye, is green and environment-friendly, and has a remarkable industrial application prospect.

The invention belongs to the field of biosynthesis, and particularly relates to saccharomyces cerevisiae engineering bacteria for producing psoralen as well as a construction method and application of the saccharomyces cerevisiae engineering bacteria. The saccharomyces cerevisiae engineering bacteria comprise a yield increasing path of DMAPP, a synthetic path of naringenin, a synthetic path of daidzein and a synthetic path of psoralen; the yield increasing approach of the DMAPP is to express mvaE, mvaS, ERG12, ERG8, ERG19 and IDI1 on a saccharomyces cerevisiae strain WAT11, and to express mvaE, mvaS, ERG12, ERG8, ERG19 and IDI1 on a saccharomyces cerevisiae strain WAT11. The synthetic route of the naringenin is that PAL, 4CL, CHS and CHI are expressed in Saccharomyces cerevisiae with yield increased by dimethyl allyl pyrophosphoric acid; the synthetic route of daidzein is that CHR, IFS and HID are expressed in saccharomyces cerevisiae synthesized by naringenin; the synthetic route of the psoralen is that PT and OC are expressed in saccharomyces cerevisiae synthesized by daidzein.

The invention discloses a cephalotaxus hainanensis Ch2OGD1 gene and application thereof, and belongs to the technical field of gene engineering. The Ch2OGD1 gene is used for coding a 2-oxoglutaric acid dependent dioxygenase (2OGD) type Pictet-Spengler enzyme, and the Pictet-Spengler enzyme is used for coding a Pictet-Spengler enzyme. Through a tobacco transient expression system and in-vitro enzyme activity determination, the Ch2OGD1 is proved to be capable of specifically catalyzing the condensation of dopamine and 4-hydroxyphenylpropanal to generate 1-phenethyl isoquinoline. The invention provides a directional preparation method of 1-phenethyl isoquinoline based on the Ch2OGD1 gene, provides a key enzyme element for biosynthesis of a cephalotaxine alkaloid precursor compound, and provides a technical basis for expanding the application of the gene in plant genetic engineering. And a potential technical path is provided for relieving the problem of resource shortage of cephalotaxine alkaloids.

A method of microbial biosynthesis of a composite includes subjecting a first culture within a bioreactor to incubation conditions, the first culture including a first bacteria that exhibit production of a structural polysaccharide, to thereby produce an organic network structure made of produced structural polysaccharide. A second culture is added to the bioreactor after the organic network structure is produced, the second culture including a second bacteria that exhibit mineralization of calcium carbonate via microbial-induced carbonate precipitation. The second bacteria produce calcium carbonate particles which precipitate on the organic network structure to produce a mineralized organic network structure as the composite. The calcium carbonate particles can be chemically converted to calcium hydroxyapatite particles.

The invention belongs to the technical field of biology, and discloses a transaminase GabT1 mutant S106I, a construction method thereof and application of the transaminase GabT1 mutant S106I in biosynthesis of 1-deoxynojirimycin. According to the invention, the 106th serine near the molecular catalytic domain of the transaminase GabT1 derived from bacillus amyloliquefaciens is mutated into isoleucine in a site-directed mutagenesis manner, so that the catalytic efficiency of the transaminase GabT1 is remarkably improved, the problem that the catalytic efficiency of the transaminase GabT1 on fructose-6-phosphoric acid is not high at present is solved, and the application of the transaminase GabT1 to the fructose-6-phosphoric acid is promoted. The yield of 1-deoxynojirimycin synthesized by the mutant GabTS106I is increased by 43.87% compared with that of a wild type. The 1-deoxynojirimycin fermentation production capability of the transaminase GabT1 mutant and the engineering strain obtained by the invention is greatly improved, and the transaminase GabT1 mutant and the engineering strain have a good industrial application prospect.

The invention discloses a genetically engineered bacterium for biosynthesis of gastrodin as well as a construction method and application of the genetically engineered bacterium. According to the genetically engineered bacterium, through overexpression of sucrose permease CscB derived from escherichia coli W, sucrose phosphorylase Basp of bifidobacterium adolescentis, uridine triphosphoryl-glucose-1-phosphate uridine acyl transferase UgpA of bifidobacterium bifidum and UDP-glycosyl transferase RsUGT of hedyotis diffusa, an exogenous path for synthesizing UDP-glucose from sucrose is constructed in a cell, and the UDP-glucose is synthesized into UDP-glucose. And the glycosylation capability of the gastrodin precursor on hydroxybenzyl alcohol is enhanced. The invention also provides a construction method of the genetically engineered bacterium and a method for synthesizing gastrodin through whole-cell catalysis by using the strain and taking p-hydroxybenzyl alcohol and cane sugar as substrates. The genetically engineered bacterium is high in gastrodin synthesis efficiency, the molar conversion rate can reach 97.8%, the highest yield reaches 3.61 g / L, and a new green manufacturing scheme is provided for solving the problems that a traditional gastrodin production method is low in efficiency, high in cost, large in pollution and the like.

The application discloses application of a SdC2'H gene of Saposhnikovia divaricata and a method for increasing coumarin content in Saposhnikovia divaricata, and belongs to the technical field of bio-agriculture. The SdC2'H gene of Saposhnikovia divaricata and the biological material containing the SdC2'H gene are applied to the regulation of coumarin synthesis in Saposhnikovia divaricata. The method for increasing coumarin content in Saposhnikovia divaricata comprises the steps of increasing the expression amount and / or activity of the SdC2'H gene in Saposhnikovia divaricata. The SdC2'H gene is successfully cloned from Saposhnikovia divaricata for the first time, and a gene overexpression vector is constructed. The SdC2'H gene is positively regulated to control the synthesis of coumarin for the first time, and overexpression of the SdC2'H gene can significantly increase the product content. This not only deepens the understanding of the molecular mechanism of related biosynthesis, but also lays an important foundation for efficient production of high-value coumarin by using genetic engineering technology.

The invention relates to the technical field of biology, in particular to a phloretin-O-monoglucoside glycosyl transferase protein DoUGT019 in dendrobium officinale as well as an encoding gene and application thereof, and discloses a phloretin-O-monoglucoside glycosyl transferase protein with an amino acid sequence as shown in SEQ ID NO: 1, which can be used for preparing a glycosyl transferase protein with an amino acid sequence as shown in SEQ ID NO: 1 in the presence of a UDP-glucose donor. The method has the advantages that the method is simple and convenient to operate and can catalyze O-glycosylation of phloretin A ring 2 '-OH, 4'-OH and B ring 4-OH, three high-value monoglucosides, namely phlorizin, trilobatin and phloretin-4-O-glucoside, are synchronously generated, multi-product synthesis can be realized without synergy of various enzymes, the process is greatly simplified, and the use cost of enzyme preparations is reduced. Meanwhile, discovery of the protein reveals a unique phloretin glycosylation metabolic pathway of dendrobium plants, and a cloning and functional verification system of the protein can also be used as a general technical template for research of similar glycosyltransferase to promote technical iteration and theoretical development in the field of glycosylation biosynthesis.

The invention provides paeonia suffruticosa-derived phosphatidyldiacylglycerol acyltransferase 1-2 or a coding gene and application thereof, and belongs to the technical field of functional proteins. The invention relates to phosphatidyldiacylglycerol acyltransferase 1-2 from paeonia ostii, which comprises phosphatidyldiacylglycerol acyltransferase 1-2 from paeonia ostii and / or phosphatidyldiacylglycerol acyltransferase 1-2 from paeonia suffruticosa. Experiments show that the Paeonia ostii-sourced phosphatidyldiacylglycerol acyltransferase 1-2 and / or Paeonia fulva-sourced phosphatidyldiacylglycerol acyltransferase 1-2 not only can synthesize linoleic acid and alpha-linolenic acid by using exogenous polyunsaturated fatty acid, but also does not affect accumulation of yeast total lipid. The peony-sourced phosphatidyldiacylglycerol acyltransferase 1-2 provided by the invention provides a new method for biosynthesis of triacylglycerol, and provides a new way for genetic breeding of oil crops.

The invention discloses a method for increasing the fermentation yield of mernomycin A based on a reinforced chassis strain glycosyl donor. Specifically, in the heterologous expression process of a sirnomycin A biosynthetic gene cluster in a chassis strain Streptomyces albus J1074, a glycosyl donor synthesis module gene and a resistance gene (MaPBP) are introduced at the same time, and on the basis of improving a glycosyl biosynthetic donor, the physiological tolerance of the chassis strain is also enhanced. Compared with a high-yield strain Streptomyces albus LX03 (CGMCC NO. 20985) of which the tolerance is only optimized, the yield of the strain Streptomyces albus LX02: pJQK610 subjected to genetic engineering optimization is improved by 285%, and the yield reaches 114 mg / L. The strain disclosed by the invention has the advantages that the yield of the strain Streptomyces albus LX02: pJQK610 is improved by 285%, and the yield of the strain Streptomyces albus LX02: pJQK610 is improved by 285%. The invention provides a high-yield technology for improving heterologous expression of the mernomycin A through collaborative optimization of precursor supply and physiological tolerance, and provides reference for efficient production of the medicine.

The invention belongs to the technical field of biology, and discloses a biosynthesis method of L-homoserine. According to the biosynthesis method, L-homoserine is synthesized by taking L-aspartic acid and formaldehyde as substrates, aldolase and transaminase are adopted, and oxaloacetic acid decarboxylase can be optionally added to improve the efficiency of a catalytic system. The method can be realized through an in-vitro multi-enzyme catalysis system, and can also be completed in a manner of introducing a way into microorganisms. According to the method, methanol, formaldehyde and other carbon resources can be introduced to synthesize the L-homoserine with high value, the raw materials are low in price, and the whole synthesis method has the advantages of high atom utilization rate and high conversion rate.

A modified sporulating bacteria may comprise a nucleic acid cassette having a silent promotor that inhibits transcription by the modified sporulating bacteria. The nucleic acid cassette may include a nucleic acid sequence including a gene of interest that encodes a protein of interest. The nucleic acid cassette may include a nucleic acid sequence encoding a loading peptide that incorporates into a coat of a spore. The modified sporulating bacteria may comprise a pre-spore having the nucleic acid sequence having the gene of interest that encodes the protein of interest. A modified spore may be formed comprising a spore body containing the nucleic acid cassette, a spore coat, and a protein of interest derived from the gene of interest inserted into the spore coat made by exogenous cell-free biosynthesis components.

The present disclosure provides a P450 cytochrome enzyme for andrographolide synthesis and its application, belonging to the field of bioengineering. The present disclosure uses Saccharomyces cerevisiae CEN.PK2-1D as a host, and implements knockout of ROX1 and GAL80 genes on the genome, and integrative expression of GGPP synthase encoding gene and CPS diterpene synthase encoding gene at ROX1 site; and implements free expression of ApCPR and CYP71A8 and CYP71D10 both with truncated signal peptides, successfully constructing recombinant S. cerevisiae, and achieving de novo synthesis of 3,15,19-Trihydroxy-8(17),13-ent-labdadiene-16-oic acid. Compared with the blank, a response value of a product peak reaches 1.9*106, and this strategy provides necessary reference for analyzing biosynthetic pathway of andrographolide and using metabolic engineering to synthesize andrographolide and related derivatives thereof.

The invention discloses ganoderic acid mass production and application based on cytochrome P450 enzyme CYP512W6, the cytochrome P450 enzyme CYP512W6 in ganoderma lucidum is converted into a corresponding saccharomyces cerevisiae strain for heterologous expression, and three kinds of ganoderic acid, namely TLTOA, 3 beta-TLTOA and DLDOA, are obtained after fermentation. The invention provides a new implementation mode for heterologous biosynthesis of ganoderic acid in saccharomyces cerevisiae.

The invention discloses a diketopiperazine heterodimer as well as a biosynthesis method and application thereof, and belongs to the technical field of bioengineering. The biosynthesis method of the diketopiperazine heterodimer comprises the following steps: S1, culturing a recombinant cell containing P450 dimerization enzyme or a mutant thereof, and performing induced expression to obtain a whole-cell culture; and S2, adding a cyclic dipeptide substrate containing tryptophan into the whole-cell culture, carrying out a catalytic reaction, and separating and purifying the obtained product to obtain the diketopiperazine heterodimer. According to the invention, the P450 dimerization enzyme or the mutant thereof is used for catalyzing the biosynthesis of the tryptophan-containing diketopiperazine into the diketopiperazine heterodimer with different regioselectivity / stereoselectivity, the biosynthesis method is green and environment-friendly, the efficiency is high, and the variety of the diketopiperazine heterodimer is greatly expanded.

The invention relates to euphausia superba-derived diacylglycerol acyltransferase as well as a coding gene and application thereof, and belongs to the field of enzymology, the nucleotide sequence of the gene for coding the enzyme is as shown in SEQ ID NO.1, and the amino acid sequence of the enzyme is as shown in SEQ ID NO.2. The invention further discloses a preparation method of the euphausia superba-derived diacylglycerol acyltransferase. The invention also provides a recombinant plasmid and a recombinant engineering bacterium containing the gene of the enzyme. Diglyceride and an acyl donor are used as substrates, and triglyceride is catalytically synthesized through an acyl transfer reaction by using the enzyme. The invention provides an efficient tool for realizing enzymatic biosynthesis and large-scale production of natural triglyceride.

The invention relates to the technical field of plant genetic engineering, and discloses a strawberry stolon regulation gene ZFP6 and application thereof, and the nucleotide sequence of the gene is as shown in SEQ ID NO.1. Researches prove that the ZFP6 gene is specifically expressed in axillary meristem of strawberry in the adult-plant stage, and the GA20ox4 gene can be directly or indirectly activated to start a biosynthesis pathway of GA and drive axillary buds to develop towards stolons. Through genetic interaction analysis of the ZFP6 gene, the GA20ox4 gene and the RGA1 gene, the function of the ZFP6 gene as an axillary bud fate decision upstream regulation factor is further verified, the blank of an endogenous regulation mechanism of the strawberry stolons is filled, and the ZFP6 gene is proved to be a molecular target for specifically regulating formation of the strawberry stolons. The gene sequence, the primer, the gene knockout expression vector and the matched technical system provided by the invention lay a foundation for strawberry molecular breeding and industrial application.

This invention belongs to the field of genetic engineering technology, specifically relating to an engineered bacterium that efficiently synthesizes curcumin using ferulic acid as a precursor, its construction method, and its application. This invention enhances ferulic acid uptake and precursor supply capabilities from the source by knocking out the fdc and mcrC genes and overexpressing the tnaT and accD genes. Furthermore, it achieves high-level stable expression of exogenous key enzyme genes DCS and CURS through codon optimization. Combined with the synergistic effect of a two-stage fermentation regulation process, a stepwise increase in ferulic acid conversion rate is achieved. In a fermentation system with an initial ferulic acid concentration of 5 g / L, the conversion rate of the recombinant strain of this invention increased from 28.6% to 55.2% compared to the unoptimized strain, and further reached 80.2% after fermentation process optimization. Simultaneously, it effectively inhibits the formation of the byproduct 4-vinylguaiacol, significantly improving the utilization rate of ferulic acid and reducing the raw material cost of curcumin biosynthesis.

The invention relates to the technical field of genetic engineering and molecular breeding, in particular to a ramie MYB transcription factor and application thereof. The MYB transcription factor is BnMYB6, the amino acid sequence of the MYB transcription factor is as shown in SEQ ID NO.1, and the gene sequence for coding the amino acid of the MYB transcription factor is as shown in SEQ ID NO.2. The transcription factor protein BnMYB6 can specifically and negatively regulate the biosynthesis of plant lignin, and has application value in the aspects of inhibiting the synthesis of plant lignin, promoting the growth and development of a root system and maintaining related traits such as the activity of the root system under drought stress.