10 results about "Microbial oil" patented technology

The application discloses a method for judging the extension of the oil reservoir swept range of a microbial polymer system, and the steps are as follows: (1) taking the oil well samples in the oil reservoir before and after the microbial polymer flooding to perform the whole hydrocarbon gas chromatographic analysis of crude oil, and determining the small molecular weight light hydrocarbon marker component reflecting the change rule of the crude oil composition; (2) comparing the chromatographic peak area of the small molecular weight light hydrocarbon marker component with the chromatographic peak area of the high molecular weight carbon number, and obtaining the parameter for characterizing the effective action of the microbial polymer; (3) comparing and analyzing the crude oil samples in the oil well before and after the parameter for characterizing the effective action of the microbial polymer, and determining the swept degree of the oil reservoir by the microbial polymer. The application discloses the application of the above method in the microbial oil production for judging the swept volume of the microbial polymer in the oil reservoir.

The present application belongs to the field of microbial metabolic engineering and industrial microorganism technology, and particularly relates to a method for stably integrating exogenous DNA into Pseudozyma roseolata, an iterative gene editing method and application thereof. The preservation number of the strain MYL-2 is CCTCC M 20252968, and the strain can efficiently accumulate oil under nitrogen-limited culture conditions, the main fatty acid components in the cells are C16-C18 fatty acids, and the strain has application potential as a microbial oil production strain. The present application provides a method for stably integrating exogenous DNA into Pseudozyma roseolata, and constructs a recombinant expression vector pNFH-loxp-RAD51 and a Cre recombination vector containing a loxP site, and for the first time, precise removal and repeated use of a screening marker mediated by a Cre-LoxP system are realized in the strain, thereby laying a foundation for multi-round gene editing and efficient engineering modification of the strain.

The invention relates to the field of fermentation, and discloses application of a metal organic framework material, a method for improving the yield of polyunsaturated fatty acid of an oil-producing microorganism and a preparation method of microbial oil. The method for improving the yield of the polyunsaturated fatty acid of the oleaginous microorganism comprises the following steps: inoculating the oleaginous microorganism into a culture medium containing a metal organic framework material for culturing; the metal organic framework material is selected from at least one of a cerium-based metal organic framework material, a copper-zinc-based metal organic framework material and a copper-based metal organic material. The method provided by the invention can improve the yield of the polyunsaturated fatty acid in the oleaginous microorganisms, and has a remarkable market prospect.

This application relates to a microbial oil composition containing retinoids and fatty acids, a cosmetic composition, a food composition, a feed composition containing the same, and a method for producing the same.

The present invention consists of a process for removing impurities from hard water. Using chemical agents, it removes impurities by mixing a chemical preparation and employing decantation in the first stage of impurity removal. Subsequent validation of the results allows for the removal of other types of impurities present in the water, such as arsenic, cadmium, cyanide, copper, chromium, mercury, nickel, lead, and zinc. Finally, it removes contaminants and microorganisms from the following group: fats and oils, suspended solids, organic carbon, total nitrogen, total phosphorus, worm eggs, E. coli, and fecal enterococci. The resulting sediments are used as biofertilizers, and the treated water can be discharged into conventional municipal sewer systems or, if necessary, treated with ozone (O3) for human consumption.

The present disclosure describes microbial oil compositions that can include a biomass present in a culture medium. The biomass can include a microbial oil enriched in EPA. The present disclosure also describes methods of producing microbial oils enriched in EPA. The present disclosure also describes nutritional oil compositions including the microbial oils and nutritional oil concentrates prepared from the nutritional oil compositions or microbial oils.

This invention provides a geophysical method and system for rapidly identifying favorable oil and gas drilling target areas in small to medium-sized basins with low exploration levels. It relates to the field of oil and gas exploration technology and aims to solve the problems of high exploration costs, long exploration times, and significant influence from topographic conditions in traditional techniques for selecting favorable drilling target areas. This invention rapidly characterizes the basin's structural pattern and target layer distribution through time-frequency electromagnetic exploration, initially delineating favorable oil and gas prospective areas. Resistivity and polarizability anomalies are obtained through inversion of time-frequency electromagnetic data to predict potential oil and gas accumulation areas. Microbial oil and gas detection is conducted in prospective areas, and the oil and gas enrichment range is predicted using light hydrocarbon micro-leakage characteristics and cross-validated with electromagnetic results. Finally, multi-attribute overlay analysis is performed on polarizability anomalies and microbial anomalies to select areas with dual or triple high anomalies as favorable oil and gas drilling target areas. This invention features low cost, high efficiency, large exploration depth, and high reliability, significantly improving drilling success rates.

The invention provides an oil reservoir microbial flooding three-dimensional physical simulation experiment device and an experiment method. The experiment device comprises an injection unit, a model unit, an extraction metering unit, a data acquisition and processing unit and a control unit, the injection unit comprises an injection pump, an intermediate container, a pressurizing device and a liquid supplementing device, the model unit comprises a model pressure cabin and an annular pressure device, an outcrop core is arranged in the model pressure cabin to serve as a three-dimensional physical model, point locations are screened on the outcrop core for drilling, and a sensor and a probe are arranged on the outcrop core; and the extraction metering unit comprises a separation device, a metering device and a pressure return device. According to the invention, a three-dimensional physical simulation experiment aiming at microbial displacement is designed, a model pressure chamber and a rock core model are taken as main bodies, oil reservoir physical characteristics and microbial growth conditions are simulated through real-time detection and control, the microbial oil displacement effect is evaluated, microbial growth and metabolism characteristics, seepage rules and oil displacement mechanism researches are deeply carried out, and the oil displacement effect is evaluated. And guidance is provided for the actual oil reservoir oil extraction process.

The application discloses a gene quantitative microorganism oil and gas exploration method based on mathematical statistics, which comprises the following steps: step 1, field sampling and sample transportation, sampling of cuttings and produced fluid and transporting to the laboratory in the fastest way. The beneficial effect is that the application discards artificial additives and instead uses the inherent microbial community in the stratum which coexists with the oil and gas environment as a 'natural tracer', which fundamentally eliminates the pollution risk and realizes absolute green environmental protection; at the same time, since no expensive reagent is needed, the main cost is concentrated in the later analysis, so that large-scale and high-frequency application becomes possible; at the same time, the microbial community structure in the application can respond and evolve in real time with the reservoir environment (such as fluid change), and through regular sampling analysis, the method can continuously capture the dynamic change of the'microbial fingerprint', and realize long-term and high-time-effect tracking of the produced fluid profile, interwell connectivity and water drive front.