101 results about "Microbial oil" patented technology

The invention relates to the technical field of microbial fermentation, and discloses application of a regulatory factor in promoting schizochytrium limacinum to produce DHA (docosahexaenoic acid), and the regulatory factor contains beta-carotene and reduced glutathione. In addition, the invention also provides a method for improving DHA production of schizochytrium limacinum and a preparation method of microbial oil. The method comprises the following steps: fermenting schizochytrium limacinum in a culture medium, and adding beta-carotene and reduced glutathione into the culture medium in the fermentation process; in the culture medium, the content of the beta-carotene is 1.5 to 2g / L. The beta-carotene and the reduced glutathione can effectively improve the content of grease and DHA prepared by fermentation of schizochytrium limacinum, the content of DHA can reach 63.9%, and the beta-carotene and the reduced glutathione are non-toxic and high in safety.

The invention discloses a method for promoting synthesis of microbial oil based on a light-operated gene and a photocatalyst, and belongs to the technical field of microbial oil. According to the invention, the oleaginous yeast Po1f-OptoZWF1 containing a light-operated gene expression system is constructed by adopting a molecular biological technology, and meanwhile, the oleaginous yeast is cultured by adopting a two-stage method (darkness and illumination). In the dark stage, sufficient nutrition is provided, and rapid proliferation of the oleaginous yeast is promoted; in the grease accumulation stage, illumination culture is converted, a photocatalyst is added, a photocatalyst-oleaginous yeast hybrid system and a light-operated gene expression system are constructed to inhibit the ZWF1 gene and a corresponding pentose phosphate pathway, and a photocatalytic material provides photo-induced electrons to promote regeneration of reducing power NADPH, so that grease synthesis is efficiently promoted. According to the method, through gene modification and a two-stage culture strategy, the yield of oil synthesis is remarkably increased, and the problems that existing oil-producing microorganisms are slow in proliferation and low in oil yield are solved.

The invention discloses a microbial oil-displacing active agent and a preparation method thereof, and belongs to the technical field of microbial oil recovery. The microbial oil-displacing active agent comprises the following components in percentage by mass: 5-15% of functionalized xanthan gum, 15-25% of a microbial agent and the balance of water, through the synergistic effect of the functionalized xanthan gum and the microbial agent, the oil displacement effect of the microbial oil displacement active agent is remarkably improved, and the functionalized xanthan gum not only serves as a thickening agent and an oil displacement agent, but also provides a good carrier and protection effect for the microbial agent, so that the microbial agent can be more uniformly distributed in an oil reservoir, and the oil displacement efficiency of the microbial agent is improved; the microbial oil displacement technology is adopted, the problem that a traditional chemical oil displacement agent pollutes the environment is solved, good environment friendliness is achieved, the functionalized xanthan gum and the microbial agent both have good biodegradability and can be naturally degraded in the oil reservoir environment, long-term environmental pollution cannot be caused, and the requirement for environmental protection is met.

The invention provides a high-temperature-resistant lipopeptide reinforced foam flooding system and a screening method and application, and relates to the technical field of microbial oil recovery, the high-temperature-resistant lipopeptide reinforced foam flooding system comprises the following steps: S1, screening applied oil reservoirs; s2, based on the oil reservoir temperature and the crude oil viscosity selected in S1, screening a high-temperature-resistant lipopeptide reinforced foam flooding system; the high-temperature-resistant lipopeptide reinforced foam oil displacement system comprises a foaming agent, a foam stabilizer, gas and water, the foaming agent comprises one or more of surfactin (C12-18), scabrin (C14-18), iturin (C14-17), deocytin, serratia marcescens and mucin fermentation liquor, and the foaming agent comprises one or more of lipopeptide, a foaming agent and a foaming agent. The foam stabilizer comprises one or more of biosynthetic nano-silver, nano-iron, nano-copper, nano-zinc, nano-zirconium and nano-nickel. The method has the beneficial effects that the oil reservoir application range is wide, the pertinence is high, the field test effect is good, and a high-temperature-resistant lipopeptide enhanced foam oil displacement system is specifically constructed according to the temperature-resistant stability characteristics of lipopeptide surfactants and biosynthetic nano materials of different structures and types.

The invention provides a medium-low permeability oil reservoir rhamnolipid foam oil displacement system and a screening method and application, and relates to the technical field of microbial oil recovery, the medium-low permeability oil reservoir rhamnolipid foam oil displacement system comprises the following steps: S1, screening an applied oil reservoir; s2, determining a rhamnolipid foam flooding system based on the oil reservoir mineralization degree; s3, based on the porosity of the oil reservoir, the injection mode of a rhamnolipid foam oil displacement system is determined, the rhamnolipid foam oil displacement system comprises a foaming agent, a foam stabilizer, nitrogen and water, the foaming agent comprises one or more of dirhamnose difat, dirhamnose monofat, single rhamnose monofat and single rhamnose difat fermentation liquor, and the foam stabilizer comprises one or more of dirhamnose difat, dirhamnose monofat, single rhamnose monofat and single rhamnose difat fermentation liquor. The foam stabilizer is prepared from one or more of xanthan gum, welan gum, Wollan gum, Dide gum, gellan gum, rhamnose gum, diidine gum, plant cellulose and bacterial cellulose. The method has the beneficial effects that the oil reservoir application range is wide, the pertinence is high, the field test effect is good, efficient generation and long-acting migration of foam in the oil reservoir are guaranteed, green and high-quality development of old oil fields is promoted, and achievement of the national dual-carbon strategy is assisted.

The invention is applicable to the technical field of resource utilization and biological refinery of plant-derived traditional Chinese medicine residues, and provides an open type fermentation grease production method based on bacteriostatic traditional Chinese medicine residues, which comprises the following steps: firstly, mixing the bacteriostatic traditional Chinese medicine residues and traditional Chinese medicine residues rich in polysaccharide, and then carrying out dilute acid pretreatment; then cellulase is added for hydrolysis to prepare traditional Chinese medicine residue hydrolysate as a fermentation culture medium, the fermentation culture medium does not need to be sterilized, the oleaginous yeast is directly inoculated for open fermentation, bacteriostatic components in the mixed medicine residues can avoid infectious microbe pollution, and fermentable sugar can be efficiently converted into microbial oil by the oleaginous yeast. The method is simple and efficient to operate, adopts an open fermentation mode, avoids sugar loss caused by high-temperature and high-pressure sterilization, remarkably reduces the process and equipment cost, improves the grease yield and the grease production efficiency, and has obvious comprehensive technical advantages. The fatty acid composition of the prepared microbial oil is similar to that of vegetable oil, and the microbial oil is suitable for being used as a biodiesel raw material.

The invention discloses bacillus velezensis S1. The preservation number of the bacillus velezensis strain S1 is CGMCC (China General Microbiological Culture Collection Center) No.28915, and the preservation number of the bacillus velezensis strain S1 is CGMCC No.28915. As a microbial oil-displacing agent, the strain is applied to microbial oil recovery of oil reservoirs with high salinity and alkalinity, oxygen deficit and even oxygen-free. The invention further discloses a preparation method of the bacillus velezensis, a preparation method of bacillus velezensis fermentation liquor and application of the bacillus velezensis S1 and the fermentation liquor in oil reservoirs which are high in salinity and alkalinity, oxygen-deficient and even oxygen-free. The S1 strain disclosed by the invention can reach a stable growth period after being cultured for about 12 hours, the relatively appropriate growth temperature is 37 DEG C, and the strain shows good growth in an environment with the pH value of 6-8, can tolerate the mineralization degree of about 100000 mg / L, and can grow under an anoxic condition, so that the S1 strain can be relatively well suitable for an oil reservoir stratum environment with high salt and alkali, oxygen deficiency and even oxygen-free conditions.

The invention discloses an enterococcus faecium XL, and the preservation number of the enterococcus faecium XL is CGMCC (China General Microbiological Culture Collection Center) No. 28133. The enterococcus faecium XL and the application thereof have the following beneficial effects: 1, the enterococcus faecium XL provided by the invention can grow and metabolize under an oil reservoir condition to generate lactic acid organic acid, dissolve carbonate in a rock stratum, increase the permeability of the rock stratum, degrade heavy components in crude oil, relieve crude oil blockage in an oil layer, increase the liquid amount and improve the oil reservoir quality; the oil recovery rate is improved; 2, the enterococcus faecium XL is applied to microorganism single-well throughput, the average single-well oil increasing amount is greater than 400 tons, and the validity period is more than 200 days; and 3, the enterococcus faecium XL is applied to microbial oil displacement, the average daily oil increase of a well group is more than 5 tons, and the water content drop amplitude is more than 5%.

The present application relates to a kind of skin care oil compositions for sensitive skin damage repair and its application, the skin care oil compositions include microbial oil and base oil, the microbial oil is with perilla seed powder, almond powder, camellia seed powder, glucose, protein peptone, yeast extract as substrate, using schizochytrium and lactobacillus plantarum to ferment substrate, the microbial oil obtained by fermentation has good effect of repairing skin damage.

The application discloses a method for judging the extension of the oil reservoir swept range of a microbial polymer system, and the steps are as follows: (1) taking the oil well samples in the oil reservoir before and after the microbial polymer flooding to perform the whole hydrocarbon gas chromatographic analysis of crude oil, and determining the small molecular weight light hydrocarbon marker component reflecting the change rule of the crude oil composition; (2) comparing the chromatographic peak area of the small molecular weight light hydrocarbon marker component with the chromatographic peak area of the high molecular weight carbon number, and obtaining the parameter for characterizing the effective action of the microbial polymer; (3) comparing and analyzing the crude oil samples in the oil well before and after the parameter for characterizing the effective action of the microbial polymer, and determining the swept degree of the oil reservoir by the microbial polymer. The application discloses the application of the above method in the microbial oil production for judging the swept volume of the microbial polymer in the oil reservoir.

The invention relates to the technical field of microbial oil recovery, in particular to bacillus subtilis 20P3 LZT, fermentation liquor and application of the bacillus subtilis 20P3 LZT to microbial oil recovery, the bacillus subtilis 20P3 LZT is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.30366. The bacillus subtilis 20P3 LZT is found for the first time, can generate surfactant lipopeptide, can grow on the basis of molasses or corn steep liquor dry powder as a carbon source, has good emulsification and viscosity reduction performance on crude oil, has the emulsification and viscosity reduction rate of 70% on thick oil, can reduce the viscosity of the crude oil and enhance the fluidity of the crude oil, and can be used for preparing crude oil. On one hand, the oil-water fluidity ratio is reduced, and on the other hand, the emulsified crude oil has the effects of profile control and liquid flow diversion, so that the crude oil recovery rate is indirectly improved, and a new strain and a material are provided for a microbial oil recovery technology.

The invention belongs to the technical field of oil reservoir microorganisms, and particularly relates to a zoococcus strain and application thereof. The bacterial strain is a Planococcus sp. Bacterial strain WL-9, and is a new strain of the Planococcus sp. The strain is separated from an oil reservoir source, can reduce interfacial tension between crude oil and water, plays a role in emulsifying the crude oil, is good in oil reservoir adaptability, and can convert the crude oil into methane in an extreme environment of the oil reservoir and in the presence of CO2. The invention provides a new high-quality strain resource for microbial oil recovery, and has great significance for improving the oil recovery rate, reducing carbon emission by CCUS microbial carbon conversion and treating and repairing petroleum pollution.

The invention discloses a method for improving the development effect of a shale oil and gas reservoir by utilizing microorganisms. The method comprises the following steps: (1) screening the shale oil and gas reservoir; (2) screening strains and activating agents thereof; (3) determining on-site injection process parameters; (4) performing composite fracturing construction; and (5) field test and effect evaluation. The method for improving the shale oil and gas reservoir development effect by using the microorganisms has the following beneficial effects: 1, the bacterial liquid and the nutritional agent thereof are injected, are water-soluble substances, are high in safety performance, and are combined with fracturing to solve the injection problem; 2, the strain acts on reservoir rocks, directly improves the porosity and permeability, and is long in period of validity, which is embodied in high primary yield and long period of validity of an oil well in production; 3, the acid-producing microorganisms are compounded, a bedding structure is utilized, the reaction effect is improved, and the dosage is low; and 4, the microbial oil displacement agent is suitable for shale oil and gas reservoirs and biological limestone reservoirs, and can be compounded with other common microbial oil displacement functional bacteria to further improve the effect.

The present invention provides a method for preparing a microbial oil containing dihomo-γ-linolenic acid, the method comprising: adding at least two types of Δ5 desaturase inhibitors to a liquid medium; and culturing a microorganism having reduced or lost Δ5 desaturase activity in the liquid medium to produce the microbial oil containing dihomo-γ-linolenic acid. The content of arachidonic acid in the microbial oil containing dihomo-γ-linolenic acid obtained by the preparation method of the present invention is lower than that in the oil obtained by the conventional method.

The invention relates to a method for reducing the salt content of salt-containing hydrolysates. The invention also relates to a method of producing a target product, preferably a microbial oil, comprising providing a hydrolysate having a reduced salt content, and culturing a microorganism, preferably an oleaginous microorganism, using a growth medium comprising the hydrolysate having a reduced salt content.

To provide an oil that contains dihomo-γ-linolenic acid at a higher purity.SOLUTION: A microbial oil is provided that is characterised by a high content of dihomo-γ-linolenic acid contained therein and / or by a reduced content of undesirable constituent fatty acid(s).SELECTED DRAWING: Figure 1

The application discloses a kind of microbial oil extraction methods using green plants as raw material, comprising the following steps: (1) target reservoir screening;(2) target reservoir endogenous microbial community structure analysis;(3) target reservoir methanogen analysis;(4) green plant injection preparation;(5) green plant injection on-site injection, the application is applicable to the field of microbial oil extraction technology, for the reservoir that complete flora exists in endogenous microorganism, on the one hand, carbon fixation is used to green plants, and the emission of carbon dioxide is reduced;On the other hand, endogenous microorganism in reservoir uses green plant injection as nutrition, and acid and gas are produced after degradation, reduce the cost of microbial oil extraction technology, greatly improve the effect of microbial oil extraction;For the reservoir without complete flora, by supplementing functional bacteria, various complex organic matter can be hydrolyzed into relatively simple organic matter, and anaerobic fermentation is carried out to produce gas, effectively improve the recovery efficiency of crude oil, improve the development effect of middle and late reservoir.

The invention is suitable for traditional Chinese medicine residue resource utilization and biological refinery technology, and provides a preparation method of a high-protein and high-fat feed. The method comprises the following steps: adding a certain amount of cheap nitrogen source into plant source wet traditional Chinese medicine residues rich in starch to prepare a fermentation culture medium, inoculating trichoderma reesei and lipomyces starkeyi for mixed culture, and performing combined action on cellulase produced by the trichoderma reesei and amylase produced by the lipomyces starkeyi on the traditional Chinese medicine residues, so as to obtain a fermentation product. And rapidly degrading into fermentable sugar which is efficiently used for microbial growth and grease and protein production, then carrying out solid-liquid separation, and drying the solid to obtain the high-protein and high-grease feed. Wet traditional Chinese medicine residues rich in starch are used as raw materials, cellulase and amylase production, enzyme hydrolysis and fermentation are highly integrated, the number of equipment is reduced, the production and processing flow is simplified, and the production cost of the high-protein feed is remarkably reduced. The fermented product serving as the feed is rich in protein and microbial oil, the fatty acid composition of the microbial oil is similar to that of vegetable oil, the nutritional ingredients of the feed are further improved, and rapid growth of poultry can be efficiently promoted. The method can promote the resource utilization of the cheap traditional Chinese medicine residues, provides a low-cost new method for improving the feed quality, and has remarkable economic benefits.

The present disclosure describes microbial oil compositions that can include biomass present in a culture medium. The biomass may include a microbial oil rich in EPA. The present disclosure also describes a method of producing an EPA-rich microbial oil. The present disclosure also describes nutritional oil compositions comprising the microbial oil, and nutritional oil concentrates prepared from the nutritional oil compositions or the microbial oil.

A self-generated biosolvent from cellulosic biorefining is used to wash / extract and optionally esterify free fatty acids from microbial oil. Free fatty acids or fatty acid esters and a hydrophobic biohydrocarbon are processed to provide a hybrid liquid fuel intermediary whereas the lipid oil is derived from cellulosic sugars and from microbial conversion of cellulose, hemicellulose, and / cellulosic sugar to lipids. In addition, higher utilization of woody biomass for the production of cellulosic sugars can be further converted to lipid oils, and the remaining hemicellulose / lignin fraction is converted to a novel hydrophobic biohydrocarbon and self-generated biosolvent in which both the processed lipid oils and biohydrocarbon can be reacted together to form a new fuel intermediary. The self-generated biosolvent comprising butanol, butanol esters, and additional biochemicals, can penetrate a microbial cell wall structure to provide higher extraction yields. The process integrates supercritical esterification of the free fatty acids using the self-generated biosolvent blend. Esterification of free fatty acids from microbial oil can be blended with a liquid biohydrocarbon to create a fuel intermediary. A phase separation process for separation of aqueous and hydrophilic materials from hydrophobic liquids is provided, wherein hydrophobic materials are the biosolvent and fatty acid esters.

Disclosed herein are processes for reducing or eliminating emulsion during the process of obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFAs) from one or more microbial cells by washing the fermentation broth with at least 1× amount of water. Further disclosed herein is microbial oil comprising one or more PUFAs that is recovered from microbial cells by at least one process described herein.

The present application relates to a kind of pseudomonas Pse-1 and its application.A kind of pseudomonas, its preservation number is CGMCC No.22583.The above-mentioned pseudomonas is applied in oil production in oil production, in microbial oil production, in the application of producing oil bacteria liquid.The beneficial effects of the present application are that (1) the pseudomonas Pse-1 provided in the present application, its growth temperature is 37~45 ℃, can be degenerated from the fluid without viscosity under reservoir condition to a kind of multifunctional oil production bacteria liquid with surface activity and viscoelasticity, also has the function of expanding swept area and washing oil, physical simulation experiment improves recovery more than 20%;(2) the fermentation broth of the pseudomonas Pse-1 is applied to microbial single well throughput, average single well oil increment is greater than 500 tons, effective period is more than 250d;(3) the fermentation broth of the pseudomonas Pse-1 is applied to microbial flooding, well group average daily oil increment is more than 5 tons.

The invention relates to the technical field of novel microbial oil-displacing agents, and particularly discloses a preparation method and application of a hydrogel nano-material immobilized microbial carrier. The preparation method comprises the following steps: combining a natural material sodium alginate, a titanium carbide nano-material and microorganisms to prepare sodium alginate composite microspheres SA-Ti3C2-g-L; compared with free microorganisms, the microbial agent has better effects in the aspects of reducing the COD of oilfield wastewater and reducing the viscosity of heavy oil, and has better application potential.

The invention belongs to the technical field of microbial oil recovery, and relates to an oil reservoir in-situ surfactant-producing flora and oil-displacing agent collaborative culture device and an oil reservoir in-situ surfactant-producing flora and oil-displacing agent collaborative culture method. Comprising a fermentation tank, the fermentation tank is provided with a PLC, the top of the fermentation tank is provided with a monitoring display unit, an oil-displacing agent quantity-controlled adding assembly and a rotary-drive stirring gas supply assembly, and the monitoring display unit, the oil-displacing agent quantity-controlled adding assembly and the rotary-drive stirring gas supply assembly are all communicated with the interior of the fermentation tank and are all electrically connected with the PLC; a temperature control heating assembly electrically connected with the PLC is arranged on the side wall of the fermentation tank body in a surrounding manner. Through cooperation of the fermentation tank, the PLC, the rotary drive stirring gas supply assembly and the oil displacement agent quantity control adding assembly, stirring and uniform mixing can be conducted in the fermentation tank, so that the liquid concentration is consistent, the dissolved oxygen value is monitored in real time, gas injection and oxygenation are automatically conducted in the fermentation tank when the dissolved oxygen value is lower than a preset value, and a favorable environment is provided for fermentation cooperative culture work; the culture effect reduction caused by the low dissolved oxygen value is avoided, and the collaborative culture effect is improved.

The present application belongs to the field of microbial metabolic engineering and industrial microorganism technology, and particularly relates to a method for stably integrating exogenous DNA into Pseudozyma roseolata, an iterative gene editing method and application thereof. The preservation number of the strain MYL-2 is CCTCC M 20252968, and the strain can efficiently accumulate oil under nitrogen-limited culture conditions, the main fatty acid components in the cells are C16-C18 fatty acids, and the strain has application potential as a microbial oil production strain. The present application provides a method for stably integrating exogenous DNA into Pseudozyma roseolata, and constructs a recombinant expression vector pNFH-loxp-RAD51 and a Cre recombination vector containing a loxP site, and for the first time, precise removal and repeated use of a screening marker mediated by a Cre-LoxP system are realized in the strain, thereby laying a foundation for multi-round gene editing and efficient engineering modification of the strain.

The present invention belongs to the technical field of microbial oil and fat nutritional products, and relates to a Sn-2 ARA oil produced by fermentation of a Mortierella alpine mutant strain and a preparation method thereof. The Mortierella alpine mutant strain is Mortierella alpine A1.0, and its deposit number is CCTCC: M20222092. The Sn-2 ARA content in the ARA oil produced by the present invention can be as high as 55.1%, and the process flow is simple, suitable for industrial-scale production, reducing environmental pollution and other problems, filling the gap in pure natural Sn-2 ARA oil.

The invention discloses a method for separating grease from oleaginous yeast and grease prepared by the method. The method comprises the following steps: culturing oleaginous yeast, and preparing fermentation liquor; carrying out spray drying and granulation on the fermentation liquor to prepare bacterial sludge microcapsule powder; and carrying out solvent extraction on the bacterial sludge microcapsule powder to prepare the grease. According to the method, the oleaginous yeast fermentation liquor is subjected to spray drying granulation, so that a high-energy-consumption dehydration step in a traditional process is simplified, the overall energy consumption is greatly reduced, and an innovative and feasible technical path is provided for efficient, energy-saving and environment-friendly industrial extraction of the microbial oil.

A method for the fractionation of fatty acids with a difference of two carbons by molecular distillation with double pass fractionation between eicosapentaenoic and docosahexaenoic acids, and compositions of microorganism oils enriched in eicosapentaenoic or docosahexaenoic acid. The method wherein (i) a reaction step between an microorganism oil including omega-3 polyunsaturated fatty acids in triglyceride form and an alcohol in the presence of a chemical or enzymatic catalyst, (ii) a first step of molecular distillation under high vacuum of the oil from step (i), in a scraper film evaporator coupled to a rectification column including at least seven theoretical plates, and recovery of a first residue and distillate, (iii) a second step of molecular distillation under high vacuum of the step (ii) residue, in the scraper film evaporator, and recovery of a second residue and distillate.

The invention discloses a bacillus subtilis SLDT20, and the preservation number of the bacillus subtilis SLDT20 is CGMCC (China General Microbiological Culture Collection Center) No.28137. The bacillus subtilis SLDT20 has the advantages that the preservation number is CGMCC No.28137; the bacillus subtilis SLDT20 has the beneficial effects that (1) the bacillus subtilis SLDT20 provided by the invention can be effectively affected in an oil reservoir, and can grow and reproduce to generate heat, so that the ratio of the viscosity of crude oil to the oil-water mobility is reduced, and the field effect is improved; (2) the conditions of sand production, casing damage and the like caused by steam channeling, continuous worsening of reservoir and underground technical conditions due to repeated scouring of steam in traditional thermal recovery are overcome, the requirements on high-temperature resistance and high-pressure resistance of oil extraction equipment and tools do not exist, the implementation method is simple, and the cost is low; (3) the bacillus subtilis SLDT20 is applied to microbial oil displacement and microbial huff and puff, the viscosity of crude oil is reduced by more than 50% in a field test, and the water content is reduced by more than 10%.

The invention belongs to the technical field of microbial oil recovery, and particularly relates to an online biological polysaccharide mixing and injecting device and method for microbial oil recovery. The device comprises a premixing unit, an electromagnetic dissociation unit, a spiral stretching unit, a mixing injection unit and an automatic control unit, and the premixing unit, the electromagnetic dissociation unit, the spiral stretching unit and the mixing injection unit are sequentially connected. The automatic control unit is connected with the premixing unit, the electromagnetic dissociation unit, the spiral stretching unit and the mixing injection unit in a wired or wireless mode. The device can realize quick and uniform mixing of biological polysaccharide and injected water, release and maintain the viscosity of a polysaccharide system, reduce the residence time and equipment investment, avoid bacterial pollution and guarantee the effect of a microbial oil recovery process.