The invention relates to a preparation method of a human
milk fat substitute, the human
milk fat substitute prepared by the method, and use of the
milk fat substitute. Specifically, edible fat is used as a
raw material, and a random ester exchange reaction, a physical mixed reaction and a directed ester exchange reaction are performed, so that the human milk
fat substitute is prepared. In the human milk
fat substitute disclosed by the invention, compared with the total weight of
fatty acid residues at an Sn-2 site of
triglyceride of the human milk
fat substitute, the content of
palmitic acid residues at the Sn-2 site is 20wt%-60wt%, and the content of
linoleic acid at the Sn-2 site is 3wt%-15wt%; compared with the total weight of the
fatty acid residues in the
triglyceride of the human milk fat substitute, the total content of the
linolenic acid is 1wt%-5wt%. According to the method disclosed by the invention, the edible fat with broad sources is used as the
raw material, and the technology is simple, so that the
mass production of the human milk fat substitute of which the structure is similar to that of
breast milk triglyceride in industry at a low cost can be realized. The human milk fat substitute prepared by the method disclosed by the invention is easy to absorb by babies, and promotion of the growth and the development of the babies is facilitated.