165 results about "Total protein" patented technology

The invention discloses application of an OsNLP3 gene, and relates to the technical field of plant genetic engineering, and an amino acid sequence coded by the OsNLP3 gene is shown as SEQ ID NO.2. By constructing an OsNLP3 gene overexpression plant and comparing the OsNLP3 gene overexpression plant with a wild type of the OsNLP3 gene overexpression plant, the increase of the single gene expression quantity of the OsNLP3 gene overexpression plant causes the significant increase of the total protein content of the rice, the invariable characters of the grain length, the grain width, the yield and the like, the genetic effect is significant, and the OsNLP3 gene overexpression plant has huge application potential and prospects for the improvement of the characters of rice varieties and provides a new gene resource for

The invention discloses a verification method for regulating and controlling proliferation and differentiation of bovine adipocytes by gene interference, which comprises the following steps: cutting bovine adipocyte tissues into small blocks, directly pasting the small blocks in a culture dish for culture, and promoting cell growth and proliferation by using nutrition and growth factors of the tissues; a ZNF423 gene is targeted by adopting an siRNA technology, siRNA is transfected into bovine fat cells by utilizing a transfection reagent, and the siRNA is an interference fragment containing si-ZNF423; the method comprises the following steps: respectively extracting total RNA (Ribonucleic Acid) and total protein of bovine adipocytes, and detecting by utilizing qPCR (Quantitative Polymerase Chain Reaction) quantitative analysis, a WB (White Batch) technology, EdU dyeing, flow cytometry detection, oil red O, BODIPY (Boron Dipyrromethene), Nile Red dyeing, TG (Triglyceride) content determination and the like. According to the invention, ZNF423 gene expression is interfered by the interference fragment containing si-ZNF423, and the effect of inhibiting proliferation and differentiation of bovine adipocytes is detected by various methods, so that the effect of the interference fragment in the bovine adipocytes is known conveniently, and regulation and control of proliferation and differentiation of the bovine adipocytes are realized by interfering gene expression by the interference fragment. The beef quality can be improved; the beef cattle breeding can be optimized.

The invention provides cream cheese and a preparation method thereof, and belongs to the technical field of dairy product processing. According to the method, firstly, raw milk is subjected to degreasing and membrane filtration concentration, the concentration multiple is controlled to be 1.5-4, the lactose content of the concentrated solution is precisely regulated and controlled to be 3-6.5% through washing filtration, and the raw milk concentrated solution rich in casein (accounting for 85-95% of total protein) is obtained. Then, mixing the concentrated solution with single cream, white granulated sugar and the like, homogenizing, sterilizing, inoculating a leavening agent and chymosin, fermenting, refrigerating and demulsifying to obtain a finished product. According to the invention, nutrition waste and environmental pollution caused by discharge of whey in a traditional process are fundamentally avoided through membrane filtration pretreatment, and meanwhile, the problems of pink feeling and peculiar smell caused by addition of milk protein powder are solved; the obtained product is pure and rich in flavor, fine and smooth in texture, simple and efficient in process and suitable for large-scale production.

The invention belongs to the technical field of biological medicines, and particularly relates to application of poecilobdella manillensis in preparation of a medicine for treating renal injury. The invention provides a new application of poecilobdella manillensis in preparation of a medicine for treating renal injury, serum biochemical index detection proves that the poecilobdella manillensis can effectively reduce serum creatinine and blood urea nitrogen levels, increase serum albumin and total protein content, remarkably improve glomerular filtration function and protein metabolism function, and improve renal function. And the improvement degree of the traditional Chinese medicine composition is superior to that of a traditional Chinese medicine contrast. Although the curative effect is slightly poorer than that of a chemical drug group, the safety advantage is remarkable. The poecilobdella manillensis can comprehensively regulate renal function metabolism indexes, relieve kidney injury and avoid common side effects of chemical drugs, provides a new choice for clinical treatment of kidney injury, and is especially suitable for chronic kidney disease patients needing long-term treatment.

The invention relates to methods for determining the concentration of an analyte in a sample obtained from processing of blood-derived plasma, the method comprising applying a light source in the near-infrared spectrum to a test sample obtained from processing of blood-derived plasma; measuring reflectance, transmission or transflectance of the test sample over a range of near-infrared wavelengths, thereby generating test wavelength spectra, comparing the test wavelength spectra with reference wavelength spectra obtained from reference samples having known concentrations of the analyte, to determine the concentration of the analyte in the sample. The invention also relates to the development of a multivariate model for use in determining the concentration of an analyte, for example, total protein or alcohol (e.g. ethanol).

The invention discloses a method for improving the solubility and thermal stability of sweet protein, and belongs to the technical field of biosynthesis, the method comprises the following steps: constructing a gene tandem recombinant plasmid containing a monellin x-3C-sfGFP-3C-monellin y expression cassette, x is greater than or equal to 1, y is greater than or equal to 1, 5 is greater than or equal to x + y is greater than or equal to 3, a 3C protease recognition sequence is also inserted between adjacent copies of monellin, and each monellin is connected with a purification tag; the gene tandem recombinant plasmid is transformed into escherichia coli and inducible expression is carried out, thalli are split and purified to obtain fusion protein, 3C protease is used for enzyme digestion, and the target protein is obtained after re-purification. The total protein yield and the solubility proportion are remarkably improved by adopting a series construction mode of plasmids, meanwhile, the stability of monellin is effectively improved, the purification step is simpler and more convenient, and the method is suitable for industrial production.

The invention relates to the technical field of beverage preparation, in particular to a yeast protein fermented beverage and a preparation method thereof. The yeast protein fermented beverage is prepared from the following raw materials in parts by weight: 37-48 parts of yeast protein, 15-25 parts of soybean protein isolate and 0.01-0.03 part of lactic acid bacteria strain. The yeast protein fermented beverage provided by the invention breaks through the limitation of a traditional dairy product fermented beverage and a common plant protein fermented beverage, uses novel plant-based food as a guide, specifically uses the yeast protein, the soybean protein isolate and the lactic acid bacteria strain as main raw materials, and creates a yeast protein fermented beverage which is excellent in flavor and good in taste through process innovation and a taste optimization technology. The yeast protein fermented high-protein beverage is high in total protein content, has the effects of increasing muscle and promoting intestinal health, and has the sensory characteristics of being high in taste fineness, good in curdling and pleasant in fermentation flavor.

A method for preparing nondenatured collagen type II for alleviating joint pain includes pulverizing a cartilage raw material and defatting to obtain a total protein crude extract; adding anhydrous ethanol to the total protein crude extract, soaking, stirring, and centrifuging to obtain an anhydrous ethanol insoluble material; dissolving the anhydrous ethanol insoluble material in water, adjusting pH to acidic, stirring and centrifuging to obtain an acid-insoluble material; dissolving the acid-insoluble material in water, performing enzymatic hydrolysis and centrifuging to obtain an enzymatic hydrolysis precipitate; dissolving the enzymatic hydrolysis precipitate in water, followed by performing co-fermentation, and centrifuging to obtain a fermentation precipitate; and adding a water activity regulator to the fermentation precipitate, and then drying to obtain the nondenatured collagen type II. The content of protein, hydroxyproline and nondenatured collagen type II in the prepared nondenatured collagen type II is high, and the shelf life is prolonged.

The invention provides modified milk powder capable of promoting bone health as well as a preparation method and application of the modified milk powder. The total protein content of the modified milk powder is 17-35 g / 100 g, the fat content of the modified milk powder is 1-32 g / 100 g, and the calcium content of the modified milk powder is 0.8-2.1 g / 100 g. Moreover, the modified milk powder contains bovine coloctrum and lactoferrin, the mass ratio of the bovine coloctrum to the lactoferrin is (5-100): 1 on the basis of the dry matter content, and the content of the lactoferrin in the modified milk powder is 2-400 mg / 100 g. The bovine colostrum and the lactoferrin are combined for preparing the milk powder, have a synergistic effect on promoting osteoblasts and inhibiting osteoclast proliferation, and are beneficial to bone health.

The invention relates to application of indole-3-propionic acid in preparation of drugs for preventing and treating pulmonary ischemia reperfusion injury. Through in-vivo animals, the inventor finds that indole-3-propionic acid can reduce the lung injury score in a concentration-dependent manner, reduce the wet weight / dry weight ratio of lungs, relieve active oxygen accumulation after lung ischemia reperfusion, and reduce the levels of total protein, tumor necrosis factor alpha and interleukin 6 in alveolar lavage fluid; the cell level also shows that the indole-3-propionic acid can improve the vitality of alveolar epithelial cells after ischemia / reperfusion injury simulation in a concentration-dependent manner, reduce release of inflammatory factors such as tumor necrosis factors alpha, interleukin 6 and interleukin 1beta, and reduce accumulation of active oxygen in the cells. Results of integrating in-vitro cells and in-vivo animals show that the indole-3-propionic acid has the effect of preventing and treating the pulmonary ischemia reperfusion injury and has a good clinical application prospect.

The invention discloses a recombinant III-type human-like collagen as well as a coding gene and an expression method thereof, and the method comprises the following steps: S1, carrying out sequence optimization by taking a human III-type collagen sequence as a template to obtain a recombinant III-type human-like collagen gene sequence; designing PCR (Polymerase Chain Reaction) amplification upstream and downstream primers according to the gene sequence, and amplifying the gene sequence of the recombinant III type human-like collagen; s2, carrying out double enzyme digestion on the recombinant III type human-like collagen gene and a plasmid, and connecting to obtain a recombinant plasmid; s3, linearizing the recombinant plasmid, transforming the linearized recombinant plasmid into agrobacterium, and screening; and S4, infecting lettuce with agrobacterium, culturing to express protein, freezing the infected lettuce with liquid nitrogen, grinding, extracting total plant protein, and purifying to obtain the recombinant III-type human-like collagen. The recombinant III type human-like collagen is expressed through lettuce, so that the method is safe and efficient, and has sufficient market value and important practical significance.

The invention discloses a protein marker for detecting or evaluating human neural stem cell paracrine and application thereof, the protein marker is a combination of SERPINE2, NCAN, VCAN, PTN, NRP2, PTPRF, C1QL1 and LDLR.The screening method comprises the steps that total protein in a human neural stem cell sample is extracted for proteome analysis and reference genome transcriptome analysis; and carrying out data processing and comparison screening to obtain common protein with high protein abundance expression and a forward regulation function, and carrying out ELISA (enzyme-linked immunosorbent assay). The human neural stem cell paracrine is detected or evaluated by detecting the expression level of the protein marker, and the application value is high.

The invention is applicable to the technical field of biology, and provides a method for rapidly extracting plant rhizome extracellular vesicles from monosaccharide and compound enzyme. According to the method, the plant extracellular vesicles are extracted by an enzymolysis-monosaccharide solution extraction coupling method for the first time, and the defect that an extraction method is single is overcome. According to the method, the conditions are mild, the primary property, the integrity and the biological activity of the vesicle structure are kept, the extraction purity is high, and the particle size distribution is uniform and concentrated in the range of 20-300 nm. The total protein extraction amounts of glucose, galactose and fructose solutions are respectively increased by 36.09% + / -1.18%, 37.65% + / -2.83% and 36.83% + / -2.59% compared with those of PBS (Phosphate Buffer Solution) extraction treatment. In addition, the'single ultracentrifugation 'purification process overcomes the problem of time consumption of traditional gradient centrifugation, and the time is shortened by more than 3h compared with that of a traditional extraction method. The method breaks through the technical bottleneck of large-scale production of the plant extracellular vesicles, and has great application and popularization values.

The invention relates to a method for preparing small-particle-size red nano-selenium particles and application of the small-particle-size red nano-selenium particles, and belongs to the technical field of selenium compounds. The nano-selenium particles contain nano-selenium and a lipoic acid oligomer. The preparation method comprises the following steps: respectively preparing nano-selenium and a lipoic acid oligomer, and uniformly mixing the nano-selenium and the lipoic acid oligomer to obtain the nano-selenium particles. The nano-selenium particles have good stability; the content of total protein and fat in highland barley grains to which the nano-selenium particles are applied is remarkably increased through detection, the nano-selenium particles become an effective means for selenium enrichment of crops, the nano-selenium particles are well applied to selenium-enriched planting of highland barley, and the application of the nano-selenium particles to the highland barley grains is developed by combining development of new selenium-enriched crop varieties and research of a new selenium fertilizer application technology. And a powerful promotion effect is achieved on the development of selenium-enriched ecological agriculture.

The invention relates to the technical field of vaccines, in particular to an activity detection method of an HPV (human papilloma virus) trivalent therapeutic vaccine. The method comprises the following steps: inoculating and infecting BEAS-2B cells by adopting constructed HPV16 / 18 / 52 type E6 / E7 antigen-loaded non-replicating type 5 adenovirus, culturing, extracting total protein, sequentially performing SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis), western blot detection, immunoreaction and developing imaging, and detecting the fusion protein; the transfection complex number MOI of the constructed adenovirus is 15-25, and the convergence degree of BEAS-2B cells is greater than 80%; the membrane transfer current of the western blot method is 220-280 mA, and the time is 50-80 minutes. The method avoids the problem of inaccurate detection results caused by obvious difference of biological activities in different animal species, has good accuracy, stability and durability, is simple in steps and low in cost, and can be effectively popularized and used.

The invention provides application of epiphyllum callus extract in preparation of cosmetics, and belongs to the technical field of new biological materials. Compared with a common epiphyllum extract, the epiphyllum callus extract provided by the invention is rich in high-content active ingredients, such as kaempferol, general flavone, total polyphenol, total protein and total sugar; the antioxidant skincare effect is achieved, and the removal efficiency of cells on DPPH free radicals can be improved. The skin care effect of promoting ATP synthesis is achieved, ATP synthesis can be remarkably promoted, and energy metabolism of damaged mitochondria is improved. Effective components of epiphyllum calluses are extracted, the effect of the epiphyllum calluses on human immortal keratinocytes is evaluated through cell experiments, it is proved that the epiphyllum calluses have excellent effects of resisting oxidation, resisting inflammation and repairing the keratinocytes, and the epiphyllum calluses are suitable for being used as raw materials to be applied to the cosmetic industry.

The invention discloses creation of a composite consists of different fruit peels followed by enrichment with omega-3 fatty acids originally derived from microorganisms of group Thraustochytrids. The process comprises the following steps: 1) collecting fresh waste of pineapple peels, banana peels, orange peels and mausambi peels, drying, removing rotten parts and grinding them to powder; 2) synergization of best combination for the creation of fruit peel wastes-based composite using response surface methodology; 3) inoculation of Aurantiochytrium cells in to the composite of fruit peel wastes and incubation at 25° C. thereby obtaining enriched composite comprising microbially-derived omega-3 fatty acids and fruit peel wastes in Solid State Fermentation conditions; 4) total protein content of the composite adjusted to at least about 30% or 40% with soyabean husk or soyabean. The final product can be processed as feed supplement to fish to enhance the fatty acids content of fishes. This invention finds suitability over prior art because the present invention is an eco-friendly, zero-waste, low cost, facile, rapid and solid waste management system.

The invention discloses application of roxburgh rose exosomes in preparation of products for preventing and treating acute alcoholic liver injury, relates to extraction of the roxburgh rose exosomes and new application of the roxburgh rose exosomes in prevention and treatment of the acute alcoholic liver injury, and belongs to the technical field of biology. The roxburgh rose exosome can also prevent and treat acute alcoholic liver injury. The particle size of the roxburgh rose exosome is 135.2 nm, the particle concentration is 1.3 * 10 < 11 > Particle / mL, and the total protein concentration is 2.12 mu g / mu L. A new thought is provided for research and development of drugs for preventing and treating acute alcoholic liver injury, the extraction method of the roxburgh rose exosome is simple, full development and utilization of food resources can be promoted, and a drug or a health care product for preventing and treating acute alcoholic liver injury is provided.

The present application relates to the technical field of cell function detection, and particularly relates to a method for evaluating cell function of HL-60 in monocyte activation test. The method comprises the following steps: (1) preparing HL-60 cell suspension; (2) stimulating with pyrogen; (3) quantifying total protein; (4) detecting by Western blotting; and (5) evaluating cell function. The method is based on Western blotting technology, and combines statistical analysis to realize objective and quantitative determination of the core functional index of the ability of synthesizing IL-6 protein under pyrogen stimulation, can accurately identify cells with pyrogen response function defects, provides clear experimental reference for selection of HL-60 cell generations, can pre-screen qualified cells, reduces pyrogen detection deviation caused by unqualified cell function and improper generations from the source, and effectively improves the reliability and repeatability of pyrogen detection results.

The invention discloses PSE-like chicken breast composite gel soft food suitable for people with dysphagia and a preparation method of the PSE-like chicken breast composite gel soft food, and belongs to the field of food processing. The raw materials of the composite gel soft food comprise PSE-like chicken breast, konjac glucomannan and a sodium chloride solution, and the preparation method comprises the following steps: adding a mixed solution of konjac glucomannan and sodium chloride into PSE-like chicken breast, stirring, carrying out heating treatment after uniform stirring, and then cooling to obtain the PSE-like chicken breast composite gel soft food. The prepared food is stable in total protein content, can effectively make up for insufficient intake of meat protein caused by limited feeding of people with dysphagia, and prevents malnutrition; the product texture meets the swallowing requirement, reaches the IDDSI level 5 or level 6 standard, can effectively reduce the risk of food residue and aspiration in the swallowing process, and has nutrition balance and food safety.

The invention provides a protein component composition with special medical application as well as a preparation method and application thereof, and relates to the field of formula food with special medical application. Wherein the protein component composition comprises protein and a sweetening agent; the protein is one or more of hydrolyzed whey protein powder, hydrolyzed casein and albumin peptide. The sweetening agent is one or more of white granulated sugar, glucose syrup, malt syrup, high fructose corn syrup, erythritol, xylitol, maltitol and stevioside. A plurality of components such as a stabilizer, mineral substances and multivitamins can also be added into the protein component composition. The finally obtained composition can be applied to preparation of formula food for special medical purposes, and the content of total protein, hemoglobin, albumin or transferrin in blood of patients suffering from malnutrition or insufficient protein intake is increased.

The present invention has been made to solve the above-described problems of conventional MP whey, and an object of the present invention is to provide a protein material containing MP whey, which can be prepared by a simple method, does not contain extra additives, and has a wide range of applications, and a method for producing the protein material.SOLUTION: The present invention provides a method for producing a protein material containing MP whey, the method including: (1) a step of preparing a whey solution having a protein concentration of 11 mass% or less and a solid content concentration of 19 mass% or less; and (2) a step of shearing the whey solution prepared in the step (1) at 70 °C or higher and 95 °C or lower. The present invention also provides a protein material containing MP whey, wherein the MP whey has a median diameter of 2 μm or more and 9 μm or less, and the ratio of the mass of the MP whey protein to the mass of all proteins is 40% or more and 85% or less.SELECTED DRAWING: None

The invention belongs to the technical field of molecular biology, and relates to an application of transferrin (TF) as an internal reference protein in preparation of a reagent for detecting exosome proteins of neurodegenerative diseases. The TF can maintain a stable expression level in nerve cells, brain tissues, blood plasma and serum-derived exosomes of a subject suffering from neurodegenerative diseases, including Alzheimer's disease, mild cognitive impairment, amyotrophic lateral sclerosis, Parkinson's disease or Huntington's disease, and also including neurodegenerative diseases such as Alzheimer's disease, mild cognitive impairment, amyotrophic lateral sclerosis, Parkinson's disease or Huntington's disease. The TF expression variation coefficient is obviously lower than that of a common exosome marker, and the expression level of TF has stronger correlation with the total protein amount of the exosome and is not influenced by external factors such as cell inflammation stress. The TF as the internal reference protein has more excellent performance, can help to more accurately reflect the total loading amount of the sample, and provides a more stable and reliable standardized tool for the field of exosome research.

The invention discloses a method for screening a medicine for treating liver injury caused by polygonum multiflorum based on biochemical indexes and application of the method. The method comprises the following steps: constructing a polygonum multiflorum induced liver injury animal model; the target drug is applied to the fleece-flower root-induced liver injury animal model, the fleece-flower root-induced liver injury animal model is administered according to a fixed time period, and the leucoglobulin ratio, the total protein content and the globulin content in the blood plasma of the fleece-flower root-induced liver injury animal model are detected. The leucoglobulin ratio, the total protein content and the globulin content in the rat plasma are used as indexes for evaluating the medicine for treating the liver injury caused by the polygonum multiflorum. According to the scheme, the method for screening the medicine for treating the liver injury of the polygonum multiflorum is simple and easy to implement, the medicine for treating the liver injury of the polygonum multiflorum can be effectively screened by taking the white ball ratio, the total protein content and the globulin content in the blood plasma of an animal with the liver injury caused by the polygonum multiflorum as detection indexes, and a theoretical basis is also provided for clinical prevention and diagnosis of the liver injury of the polygonum multiflorum.

The present invention belongs to the field of bioengineering technology, and discloses a preparation method and application of an amniotic membrane extract. Fresh amniotic membrane is disinfected, cleaned, and dried in sequence, and then crushed in an ice bath. Then, the crushed liquid is centrifuged and filtered to obtain the amniotic membrane extract. The dry matter of the amniotic membrane extract meets the following requirements: total protein content ≥78.02%, total sugar content ≥10.87%, fat content ≤3.53%, and ignition residue content ≤1.64%. The present invention improves the extraction efficiency of total protein and total sugar in the amniotic membrane extract by changing the existing production process of frozen crushing or liquid crushing, crushing the amniotic membrane in an ice-water mixture, and combining the control of process parameters such as crushing, which is beneficial to the promotion and application of the amniotic membrane extract in the preparation of ocular preparations such as ocular surface lubricating liquid, and improves the clinical use effect. In addition, the amniotic membrane extract can also be used as a functional component of biological amniotic membrane preservation liquid and combined with amniotic membrane covering surgery for the treatment of ocular surface diseases.

The present invention relates to a method for determining the concentration of an analyte in a sample obtained from a treated blood-derived plasma, the method comprising applying a light source in near infrared spectroscopy to a test sample obtained from a treated blood-derived plasma; the reflectance, transmittance, or translucency of the test sample in the range of near infrared wavelengths is measured to produce a test wavelength spectrum, which is compared to a reference wavelength spectrum obtained from a reference sample having a known concentration of the analyte to determine the concentration of the analyte in the sample. The invention also relates to the development of a multivariable model for determining the concentration of an analyte, such as total protein or alcohol, such as ethanol.

The invention relates to a method for extracting intracellular substances of microorganisms in cigar tobacco leaves. The method comprises the following steps: extracting thalli from cigar tobacco leaves; extracting nucleic acid and / or protein from the thalli; wherein the extraction method of the protein comprises the following steps: putting the thalli into a protein extraction buffer solution with the pH value of 7.0-8.5, and carrying out digestion at 40-50 DEG C to extract the protein; the extraction method of the nucleic acid comprises the following steps: putting the thalli into a nucleic acid extraction buffer solution, carrying out ultrasonication treatment for 10-60 minutes at 200-800 W, and extracting the nucleic acid. According to the method, high-purity and high-activity microbial total nucleic acid and / or total protein can be obtained from cigar tobacco leaves.

A method of treating exocrine pancreatic insufficiency includes administering to an individual in need thereof (e.g., an individual with cystic fibrosis) an effective amount of an enteral composition having a total protein consisting essentially of hydrolyzed protein and a total fat containing monoacylglycerols (MAG) that are at least about 30 wt. % of the total fat, and the total fat optionally further contains medium chain triglycerides (MCT) and / or fatty acids. In one particular non-limiting embodiment, about 60 wt. % of the total fat is from MCT and about 40 wt. % of the total fat is from MAG; in another particular non-limiting embodiment, the total fat is MAG with free fatty acids (esterified and / or bound) without MCT; and in yet another particular non-limiting embodiment, the total fat is MAG alone without MCT.

The invention discloses an effect and mechanism research method of GOT1 in lung adenocarcinoma cells, and relates to the technical field of biomedical research. Comprising the following steps: S1, cell culture and protein extraction: culturing Bas-2b, A549, H1573 and Calu-3 cells, cleaning the cells with PBS, performing pyrolysis treatment, collecting supernate as a total protein solution, and determining the protein concentration per tube by BCA; s2, carrying out Western Blot experiment, taking 10 micrograms of protein as a loading amount, carrying out electrophoresis, transferring a film, sealing, incubating a primary antibody and a secondary antibody, exposing and developing, and storing a picture; the experiment is repeated for multiple times, and Image J is used for analyzing the strip exposure diagram. According to the method disclosed by the invention, effective knock-down of GOT1 in A549 and H1573 cells is successfully realized through the steps of screening proper cell screening concentration by puromycin, exploring an MOI value, transfecting lentivirus, screening puromycin and the like; the invention provides an important tool and method for follow-up research on the function of GOT1 in lung adenocarcinoma cells.

The invention belongs to the technical field of feed. The invention provides a feed for improving the color and luster of eggs and the content of natural carotene as well as a preparation method and application of the feed. The feed comprises a basal feed and a feed additive, wherein the feed additive comprises blakeslea trispora and chili powder. The egg yolk chromaticity of eggs and the content of beta-carotene in the egg yolk can be remarkably improved. Meanwhile, the total egg protein content can be increased, the total cholesterol and moisture content can be reduced, and the technical effect of improving the egg quality is achieved.