115 results about "Total protein" patented technology

The invention provides cream cheese and a preparation method thereof, and belongs to the technical field of dairy product processing. According to the method, firstly, raw milk is subjected to degreasing and membrane filtration concentration, the concentration multiple is controlled to be 1.5-4, the lactose content of the concentrated solution is precisely regulated and controlled to be 3-6.5% through washing filtration, and the raw milk concentrated solution rich in casein (accounting for 85-95% of total protein) is obtained. Then, mixing the concentrated solution with single cream, white granulated sugar and the like, homogenizing, sterilizing, inoculating a leavening agent and chymosin, fermenting, refrigerating and demulsifying to obtain a finished product. According to the invention, nutrition waste and environmental pollution caused by discharge of whey in a traditional process are fundamentally avoided through membrane filtration pretreatment, and meanwhile, the problems of pink feeling and peculiar smell caused by addition of milk protein powder are solved; the obtained product is pure and rich in flavor, fine and smooth in texture, simple and efficient in process and suitable for large-scale production.

The invention belongs to the technical field of biological medicines, and particularly relates to application of poecilobdella manillensis in preparation of a medicine for treating renal injury. The invention provides a new application of poecilobdella manillensis in preparation of a medicine for treating renal injury, serum biochemical index detection proves that the poecilobdella manillensis can effectively reduce serum creatinine and blood urea nitrogen levels, increase serum albumin and total protein content, remarkably improve glomerular filtration function and protein metabolism function, and improve renal function. And the improvement degree of the traditional Chinese medicine composition is superior to that of a traditional Chinese medicine contrast. Although the curative effect is slightly poorer than that of a chemical drug group, the safety advantage is remarkable. The poecilobdella manillensis can comprehensively regulate renal function metabolism indexes, relieve kidney injury and avoid common side effects of chemical drugs, provides a new choice for clinical treatment of kidney injury, and is especially suitable for chronic kidney disease patients needing long-term treatment.

The invention relates to methods for determining the concentration of an analyte in a sample obtained from processing of blood-derived plasma, the method comprising applying a light source in the near-infrared spectrum to a test sample obtained from processing of blood-derived plasma; measuring reflectance, transmission or transflectance of the test sample over a range of near-infrared wavelengths, thereby generating test wavelength spectra, comparing the test wavelength spectra with reference wavelength spectra obtained from reference samples having known concentrations of the analyte, to determine the concentration of the analyte in the sample. The invention also relates to the development of a multivariate model for use in determining the concentration of an analyte, for example, total protein or alcohol (e.g. ethanol).

The invention discloses a method for improving the solubility and thermal stability of sweet protein, and belongs to the technical field of biosynthesis, the method comprises the following steps: constructing a gene tandem recombinant plasmid containing a monellin x-3C-sfGFP-3C-monellin y expression cassette, x is greater than or equal to 1, y is greater than or equal to 1, 5 is greater than or equal to x + y is greater than or equal to 3, a 3C protease recognition sequence is also inserted between adjacent copies of monellin, and each monellin is connected with a purification tag; the gene tandem recombinant plasmid is transformed into escherichia coli and inducible expression is carried out, thalli are split and purified to obtain fusion protein, 3C protease is used for enzyme digestion, and the target protein is obtained after re-purification. The total protein yield and the solubility proportion are remarkably improved by adopting a series construction mode of plasmids, meanwhile, the stability of monellin is effectively improved, the purification step is simpler and more convenient, and the method is suitable for industrial production.

A method for preparing nondenatured collagen type II for alleviating joint pain includes pulverizing a cartilage raw material and defatting to obtain a total protein crude extract; adding anhydrous ethanol to the total protein crude extract, soaking, stirring, and centrifuging to obtain an anhydrous ethanol insoluble material; dissolving the anhydrous ethanol insoluble material in water, adjusting pH to acidic, stirring and centrifuging to obtain an acid-insoluble material; dissolving the acid-insoluble material in water, performing enzymatic hydrolysis and centrifuging to obtain an enzymatic hydrolysis precipitate; dissolving the enzymatic hydrolysis precipitate in water, followed by performing co-fermentation, and centrifuging to obtain a fermentation precipitate; and adding a water activity regulator to the fermentation precipitate, and then drying to obtain the nondenatured collagen type II. The content of protein, hydroxyproline and nondenatured collagen type II in the prepared nondenatured collagen type II is high, and the shelf life is prolonged.

The invention provides modified milk powder capable of promoting bone health as well as a preparation method and application of the modified milk powder. The total protein content of the modified milk powder is 17-35 g / 100 g, the fat content of the modified milk powder is 1-32 g / 100 g, and the calcium content of the modified milk powder is 0.8-2.1 g / 100 g. Moreover, the modified milk powder contains bovine coloctrum and lactoferrin, the mass ratio of the bovine coloctrum to the lactoferrin is (5-100): 1 on the basis of the dry matter content, and the content of the lactoferrin in the modified milk powder is 2-400 mg / 100 g. The bovine colostrum and the lactoferrin are combined for preparing the milk powder, have a synergistic effect on promoting osteoblasts and inhibiting osteoclast proliferation, and are beneficial to bone health.

The invention is applicable to the technical field of biology, and provides a method for rapidly extracting plant rhizome extracellular vesicles from monosaccharide and compound enzyme. According to the method, the plant extracellular vesicles are extracted by an enzymolysis-monosaccharide solution extraction coupling method for the first time, and the defect that an extraction method is single is overcome. According to the method, the conditions are mild, the primary property, the integrity and the biological activity of the vesicle structure are kept, the extraction purity is high, and the particle size distribution is uniform and concentrated in the range of 20-300 nm. The total protein extraction amounts of glucose, galactose and fructose solutions are respectively increased by 36.09% + / -1.18%, 37.65% + / -2.83% and 36.83% + / -2.59% compared with those of PBS (Phosphate Buffer Solution) extraction treatment. In addition, the'single ultracentrifugation 'purification process overcomes the problem of time consumption of traditional gradient centrifugation, and the time is shortened by more than 3h compared with that of a traditional extraction method. The method breaks through the technical bottleneck of large-scale production of the plant extracellular vesicles, and has great application and popularization values.

The invention relates to a method for preparing small-particle-size red nano-selenium particles and application of the small-particle-size red nano-selenium particles, and belongs to the technical field of selenium compounds. The nano-selenium particles contain nano-selenium and a lipoic acid oligomer. The preparation method comprises the following steps: respectively preparing nano-selenium and a lipoic acid oligomer, and uniformly mixing the nano-selenium and the lipoic acid oligomer to obtain the nano-selenium particles. The nano-selenium particles have good stability; the content of total protein and fat in highland barley grains to which the nano-selenium particles are applied is remarkably increased through detection, the nano-selenium particles become an effective means for selenium enrichment of crops, the nano-selenium particles are well applied to selenium-enriched planting of highland barley, and the application of the nano-selenium particles to the highland barley grains is developed by combining development of new selenium-enriched crop varieties and research of a new selenium fertilizer application technology. And a powerful promotion effect is achieved on the development of selenium-enriched ecological agriculture.

The invention provides application of epiphyllum callus extract in preparation of cosmetics, and belongs to the technical field of new biological materials. Compared with a common epiphyllum extract, the epiphyllum callus extract provided by the invention is rich in high-content active ingredients, such as kaempferol, general flavone, total polyphenol, total protein and total sugar; the antioxidant skincare effect is achieved, and the removal efficiency of cells on DPPH free radicals can be improved. The skin care effect of promoting ATP synthesis is achieved, ATP synthesis can be remarkably promoted, and energy metabolism of damaged mitochondria is improved. Effective components of epiphyllum calluses are extracted, the effect of the epiphyllum calluses on human immortal keratinocytes is evaluated through cell experiments, it is proved that the epiphyllum calluses have excellent effects of resisting oxidation, resisting inflammation and repairing the keratinocytes, and the epiphyllum calluses are suitable for being used as raw materials to be applied to the cosmetic industry.

The invention discloses application of roxburgh rose exosomes in preparation of products for preventing and treating acute alcoholic liver injury, relates to extraction of the roxburgh rose exosomes and new application of the roxburgh rose exosomes in prevention and treatment of the acute alcoholic liver injury, and belongs to the technical field of biology. The roxburgh rose exosome can also prevent and treat acute alcoholic liver injury. The particle size of the roxburgh rose exosome is 135.2 nm, the particle concentration is 1.3 * 10 < 11 > Particle / mL, and the total protein concentration is 2.12 mu g / mu L. A new thought is provided for research and development of drugs for preventing and treating acute alcoholic liver injury, the extraction method of the roxburgh rose exosome is simple, full development and utilization of food resources can be promoted, and a drug or a health care product for preventing and treating acute alcoholic liver injury is provided.

The present application relates to the technical field of cell function detection, and particularly relates to a method for evaluating cell function of HL-60 in monocyte activation test. The method comprises the following steps: (1) preparing HL-60 cell suspension; (2) stimulating with pyrogen; (3) quantifying total protein; (4) detecting by Western blotting; and (5) evaluating cell function. The method is based on Western blotting technology, and combines statistical analysis to realize objective and quantitative determination of the core functional index of the ability of synthesizing IL-6 protein under pyrogen stimulation, can accurately identify cells with pyrogen response function defects, provides clear experimental reference for selection of HL-60 cell generations, can pre-screen qualified cells, reduces pyrogen detection deviation caused by unqualified cell function and improper generations from the source, and effectively improves the reliability and repeatability of pyrogen detection results.

The invention discloses PSE-like chicken breast composite gel soft food suitable for people with dysphagia and a preparation method of the PSE-like chicken breast composite gel soft food, and belongs to the field of food processing. The raw materials of the composite gel soft food comprise PSE-like chicken breast, konjac glucomannan and a sodium chloride solution, and the preparation method comprises the following steps: adding a mixed solution of konjac glucomannan and sodium chloride into PSE-like chicken breast, stirring, carrying out heating treatment after uniform stirring, and then cooling to obtain the PSE-like chicken breast composite gel soft food. The prepared food is stable in total protein content, can effectively make up for insufficient intake of meat protein caused by limited feeding of people with dysphagia, and prevents malnutrition; the product texture meets the swallowing requirement, reaches the IDDSI level 5 or level 6 standard, can effectively reduce the risk of food residue and aspiration in the swallowing process, and has nutrition balance and food safety.

The invention provides a protein component composition with special medical application as well as a preparation method and application thereof, and relates to the field of formula food with special medical application. Wherein the protein component composition comprises protein and a sweetening agent; the protein is one or more of hydrolyzed whey protein powder, hydrolyzed casein and albumin peptide. The sweetening agent is one or more of white granulated sugar, glucose syrup, malt syrup, high fructose corn syrup, erythritol, xylitol, maltitol and stevioside. A plurality of components such as a stabilizer, mineral substances and multivitamins can also be added into the protein component composition. The finally obtained composition can be applied to preparation of formula food for special medical purposes, and the content of total protein, hemoglobin, albumin or transferrin in blood of patients suffering from malnutrition or insufficient protein intake is increased.

The present invention has been made to solve the above-described problems of conventional MP whey, and an object of the present invention is to provide a protein material containing MP whey, which can be prepared by a simple method, does not contain extra additives, and has a wide range of applications, and a method for producing the protein material.SOLUTION: The present invention provides a method for producing a protein material containing MP whey, the method including: (1) a step of preparing a whey solution having a protein concentration of 11 mass% or less and a solid content concentration of 19 mass% or less; and (2) a step of shearing the whey solution prepared in the step (1) at 70 °C or higher and 95 °C or lower. The present invention also provides a protein material containing MP whey, wherein the MP whey has a median diameter of 2 μm or more and 9 μm or less, and the ratio of the mass of the MP whey protein to the mass of all proteins is 40% or more and 85% or less.SELECTED DRAWING: None

The invention belongs to the technical field of molecular biology, and relates to an application of transferrin (TF) as an internal reference protein in preparation of a reagent for detecting exosome proteins of neurodegenerative diseases. The TF can maintain a stable expression level in nerve cells, brain tissues, blood plasma and serum-derived exosomes of a subject suffering from neurodegenerative diseases, including Alzheimer's disease, mild cognitive impairment, amyotrophic lateral sclerosis, Parkinson's disease or Huntington's disease, and also including neurodegenerative diseases such as Alzheimer's disease, mild cognitive impairment, amyotrophic lateral sclerosis, Parkinson's disease or Huntington's disease. The TF expression variation coefficient is obviously lower than that of a common exosome marker, and the expression level of TF has stronger correlation with the total protein amount of the exosome and is not influenced by external factors such as cell inflammation stress. The TF as the internal reference protein has more excellent performance, can help to more accurately reflect the total loading amount of the sample, and provides a more stable and reliable standardized tool for the field of exosome research.

The invention discloses a method for screening a medicine for treating liver injury caused by polygonum multiflorum based on biochemical indexes and application of the method. The method comprises the following steps: constructing a polygonum multiflorum induced liver injury animal model; the target drug is applied to the fleece-flower root-induced liver injury animal model, the fleece-flower root-induced liver injury animal model is administered according to a fixed time period, and the leucoglobulin ratio, the total protein content and the globulin content in the blood plasma of the fleece-flower root-induced liver injury animal model are detected. The leucoglobulin ratio, the total protein content and the globulin content in the rat plasma are used as indexes for evaluating the medicine for treating the liver injury caused by the polygonum multiflorum. According to the scheme, the method for screening the medicine for treating the liver injury of the polygonum multiflorum is simple and easy to implement, the medicine for treating the liver injury of the polygonum multiflorum can be effectively screened by taking the white ball ratio, the total protein content and the globulin content in the blood plasma of an animal with the liver injury caused by the polygonum multiflorum as detection indexes, and a theoretical basis is also provided for clinical prevention and diagnosis of the liver injury of the polygonum multiflorum.

The invention relates to a method for extracting intracellular substances of microorganisms in cigar tobacco leaves. The method comprises the following steps: extracting thalli from cigar tobacco leaves; extracting nucleic acid and / or protein from the thalli; wherein the extraction method of the protein comprises the following steps: putting the thalli into a protein extraction buffer solution with the pH value of 7.0-8.5, and carrying out digestion at 40-50 DEG C to extract the protein; the extraction method of the nucleic acid comprises the following steps: putting the thalli into a nucleic acid extraction buffer solution, carrying out ultrasonication treatment for 10-60 minutes at 200-800 W, and extracting the nucleic acid. According to the method, high-purity and high-activity microbial total nucleic acid and / or total protein can be obtained from cigar tobacco leaves.

A method of treating exocrine pancreatic insufficiency includes administering to an individual in need thereof (e.g., an individual with cystic fibrosis) an effective amount of an enteral composition having a total protein consisting essentially of hydrolyzed protein and a total fat containing monoacylglycerols (MAG) that are at least about 30 wt. % of the total fat, and the total fat optionally further contains medium chain triglycerides (MCT) and / or fatty acids. In one particular non-limiting embodiment, about 60 wt. % of the total fat is from MCT and about 40 wt. % of the total fat is from MAG; in another particular non-limiting embodiment, the total fat is MAG with free fatty acids (esterified and / or bound) without MCT; and in yet another particular non-limiting embodiment, the total fat is MAG alone without MCT.

The invention discloses an effect and mechanism research method of GOT1 in lung adenocarcinoma cells, and relates to the technical field of biomedical research. Comprising the following steps: S1, cell culture and protein extraction: culturing Bas-2b, A549, H1573 and Calu-3 cells, cleaning the cells with PBS, performing pyrolysis treatment, collecting supernate as a total protein solution, and determining the protein concentration per tube by BCA; s2, carrying out Western Blot experiment, taking 10 micrograms of protein as a loading amount, carrying out electrophoresis, transferring a film, sealing, incubating a primary antibody and a secondary antibody, exposing and developing, and storing a picture; the experiment is repeated for multiple times, and Image J is used for analyzing the strip exposure diagram. According to the method disclosed by the invention, effective knock-down of GOT1 in A549 and H1573 cells is successfully realized through the steps of screening proper cell screening concentration by puromycin, exploring an MOI value, transfecting lentivirus, screening puromycin and the like; the invention provides an important tool and method for follow-up research on the function of GOT1 in lung adenocarcinoma cells.

The invention belongs to the technical field of feed. The invention provides a feed for improving the color and luster of eggs and the content of natural carotene as well as a preparation method and application of the feed. The feed comprises a basal feed and a feed additive, wherein the feed additive comprises blakeslea trispora and chili powder. The egg yolk chromaticity of eggs and the content of beta-carotene in the egg yolk can be remarkably improved. Meanwhile, the total egg protein content can be increased, the total cholesterol and moisture content can be reduced, and the technical effect of improving the egg quality is achieved.

The invention discloses a recombinant duck plague virus protein combination, a subunit vaccine as well as a preparation method and application of the subunit vaccine, and belongs to the technical field of vaccine preparation. According to the technical scheme, the recombinant duck plague virus protein combination comprises recombinant gE protein and recombinant gI protein; the amino acid sequence of the recombinant gE protein is as shown in SEQ ID NO: 10, and the amino acid sequence of the recombinant gI protein is as shown in SEQ ID NO: 13. The nucleotide sequence of the nucleic acid molecule for coding the recombinant gE protein is as shown in SEQ ID NO: 12; the nucleotide sequence of the nucleic acid molecule for coding the recombinant gI protein is as shown in SEQ ID NO: 15. The duck plague subunit vaccine provided by the invention adopts gE and gI co-expression, only contains part of antigens of DPV, does not contain genetic materials, does not have toxin expelling and dispersing risks, is good in safety, can enable the spatial conformation of gE protein to be close to that of natural protein by co-expression of gE and gI, can realize 10 / 10 protection of challenge, has the total protein expression amount of about 4000 [mu] g / ml, and can be used for preparing a vaccine for the duck plague. The culture medium can be diluted by 40 times for seedling production, so that the production cost is greatly reduced.

The invention belongs to the technical field of biology, and particularly relates to a trichoderma reesei expression site H2 and application thereof as well as a recombinant trichoderma reesei strain for producing thaumatin II protein as well as a construction method and application of the recombinant trichoderma reesei strain. The Trichoderma reesei expression site H2 provided by the invention is located at the 210th site in a gene segment as shown in SEQ ID NO.6 in a Trichoderma reesei genome, and an expression level equivalent to that of a cbh1 site can be obtained by inserting an exogenous gene into the site. The recombinant trichoderma reesei strain for producing the thaumatin II protein provided by the invention contains a gene for coding the thaumatin II protein, which has a nucleotide sequence shown as SEQ ID NO.1, and the gene has high translation efficiency in the trichoderma reesei, so that the recombinant trichoderma reesei strain can efficiently produce the thaumatin II protein, and the proportion of the thaumatin II protein in the obtained total protein is high; the method has important significance in reducing the production cost of the thaumatin II protein and promoting the application of the thaumatin II protein in the food industry.

The invention discloses a method for improving the stability of peanut butter, and belongs to the technical field of food processing. The invention provides a solution aiming at the problems that the peanut butter is easy to separate out grease and harden in texture in the storage process. The method comprises the following steps: by taking peanut protein powder as a raw material, preparing peanut protein isolate by an alkali-solution and acid-isolation method; measuring the contents of protein, fat and water in the peanut kernels, selecting baking temperature and time according to the measurement result, baking, removing red skin, and grinding to obtain a peanut butter base material; measuring the wet basis protein content and the moisture content of the peanut butter base material, selecting the addition amount of the peanut protein isolate in a range of 2-3% of the mass of the base material, and adding the peanut protein isolate into the base material to enable the wet basis total protein content of the mixture to reach 30-33%; and finally, stirring and mixing the mixture, and standing to obtain the stabilized peanut butter. The method is mainly used for improving the grease separation problem of the peanut butter and improving the storage stability of the peanut butter.

The invention discloses application of a ZmEREB93 gene knockout biological material in cultivation of high-yield and high-quality corn, and relates to the technical field of biology. The ZmEREB93 gene knockout strain is constructed, homozygous seeds of the ZmEREB93 gene knockout strain are subjected to seed test analysis, and it is found that corn kernels of the knockout strain are remarkably superior to a wild type in the aspects of the grain length, the grain width, the hundred-grain weight and the single plant yield. In the early, middle and late periods of grain filling, the corn grains of the knockout strain are obviously superior to a wild type control in shape and endosperm structure. Further determination of starch content and protein content shows that the starch content of seeds of the knockout strain is significantly higher than that of a wild type control group, the alcohol-soluble protein content is also significantly increased, and the total protein content is significantly higher than that of a control group. Therefore, the ZmEREB93 gene has an important application value in cultivation of a high-yield and high-quality new corn variety.

The exosome-glucan compound preparation provided by the invention comprises a stem cell exosome, the final concentration of the stem cell exosome in the exosome-glucan compound preparation is 1 * 10 < 8 > parts / mL to 1 * 10 < 12 > parts / mL based on particle concentration quantification or 1 mu g / mL to 500 mu g / mL based on exosome total protein quantification, and the final concentration of the stem cell exosome in the exosome-glucan compound preparation is 1 * 10 < 8 > parts / mL to 1 * 10 < 12 > parts / mL based on particle concentration quantification or 1 mu g / mL to 500 mu g / mL based on particle concentration quantification. The dextran is used as a matrix entrapment stem cell exosome, the weight-average molecular weight of the dextran is 10kDa to 5000kD, and the weight / volume percent of the dextran in the exosome-dextran compound preparation is 0.5% to 20%; glucan is used as a biocompatible matrix, and a lipid bilayer structure of the glucan wraps the stem cell exosome to form a physical protection barrier, so that the stem cell exosome is prevented from being degraded and inactivated in an in-vitro storage or in-vivo complex environment; and the degradation rate of the glucan matrix is matched with the wound healing period, so that long-acting slow release of the exosome is realized, and the bioavailability of a focus part is improved.

The present application relates to the biomedical field, disclose a kind of method for removing vitamin D in horse serum, comprising: material screening;25(OH)D in horse serum is physically adsorbed using selected material;After physical adsorption, horse serum is centrifuged;After centrifugation, horse serum is filtered using filter membrane;Selected material is dextran treatment modified activated carbon, dextran treatment modified activated carbon is repeatedly obtained by immersing treatment of modified activated carbon in saturated dextran solution;The mass ratio of dextran and modified activated carbon is 2-10:1;Modified activated carbon is obtained by bonding hydrophobic activated carbon using nitric acid pretreatment activated carbon and diphenyl dimethoxysilane, then using phytic acid to treat hydrophobic activated carbon and calcining to make, the method is efficient, low cost, can remove 25(OH)D in horse serum in scale, it also has high specificity, only remove 25(OH)D, retain total protein in horse serum;Simple operation, without complex equipment.

The invention relates to methods and kits for assessing brain injury, e.g., traumatic brain injury resulting from blast exposure. The invention provides methods of quantifying the amount of phosphorylated tau or total tau in a biological sample. The invention further provides a method of determining the number of blast exposures experienced by a subject. Also provided herein are kits for detecting phosphorylated tau or total tau in a biological sample.

The invention discloses an electrochemiluminescence detection method of PARP enzyme, a kit and application thereof. The electrochemical luminescence detection method for detecting PARP enzyme comprises the following steps: (1) mixing human whole blood, a PARP inhibitor and a PARG inhibitor, incubating, and centrifuging to obtain human peripheral blood mononuclear cells; (2) collecting a human peripheral blood mononuclear cell precipitate for cracking, detecting the total protein concentration, adding a sample, adding a cell lysis solution into an electrochemical luminescent plate for incubation, and adding a PARP enzyme detection antibody for re-incubation; and (3) adding a detection secondary antibody for incubation to form a capture antibody-PARP enzyme-detection antibody-secondary antibody complex, and adding plate reading liquid for plate reading. The electrochemical luminescence detection method and the kit have the characteristics of high sensitivity, high specificity, high accuracy, high precision and simple operation method, and can quantitatively detect the content of PARP enzyme.

The invention discloses a preparation method of high-water-binding-capacity bean-grain-based plant meat, which comprises the following steps: taking soybean protein and cereal protein as raw materials, and finally extruding the raw materials to obtain the high-water-binding-capacity plant meat through extrusion combined with continuous microwave assistance. In the raw materials, the rice protein accounts for 5-20% of the total protein of the raw materials in percentage by mass. According to the method disclosed by the invention, the plant meat product with high water binding capacity is extruded, the water binding capacity of the plant meat is obviously improved, the product characteristics of the bean-grain-based plant meat are improved, and the requirements of consumers on the taste and the quality of the plant meat are met. The continuous microwave method in the extrusion process provided by the invention is simple, harmless and easy to implement, and a new method is provided for expanding the raw material category and market of plant meat products.

To provide a processed food containing a meat-like ingredient mainly made of plant raw materials and having a meat-like richness imparted thereto, and a method for producing the same. [Solution] A heat-treated, containerized processed food containing a meat-like ingredient, wherein the meat-like ingredient is primarily made of plant-based ingredients including plant protein, and the processed food further contains acetic acid bacteria cells, the plant protein content of the total protein being 90% by mass or more, the mass ratio of the bacterial cells to the plant protein being 0.0005 or more, and the aldehyde dehydrogenase specific activity per 1 mg of the bacterial cells in a dry state being 0.1 U / mg or less.