769 results about "Total protein" patented technology

A high-protein beverage is made with a protein content of at least 15% of the total product content. At least 10% of the total product consists of hydrolyzed gelatin. A method of producing a high-protein beverage product via UHT processing comprising at least 15% total protein also is disclosed.

The invention provides an inactivated Zika virus vaccine. The inactivated Zika virus vaccine is obtained by: performing ultrafiltration and concentration on Zika virus liquid after inactivation, centrifuging the concentrated virus liquid by adopting a sucrose density zone, performing ion exchange and concentration sterilization on a centrifugal product to obtain a Zika virus vaccine stock solution, diluting the vaccine stock solution until the total protein content is not more than 20mu g/ml, and adding an adjuvant to obtain a vaccine semi-finished product. The method for preparing the vaccine provided by the invention is simple, convenient and easy to operate, the cost is saved, the produced vaccine is suitable for Asian people, a unit dose of the Zika virus liquid is high in immunogenicity, the content of hybrid protein is low, the side effect after injection is small, and the safety is high, so that the vaccine is suitable for vaccination of fertile women before pregnancy, can avoid newborn Brazil microcephaly caused by infection of Zika virus, and is significant in social value and market efficiency.

Methods of making protein-fortified yogurt products are described. The methods may include mixing a casein-containing ingredient with starting milk to make a yogurt milk, where the casein-containing ingredient has a casein-to-whey protein ratio of 82:18 or greater. The yogurt milk may be fermented after adding yogurt culture to make a yogurt mixture. The yogurt mixture may then be formed into the protein-fortified yogurt product. The total protein concentration in the protein-fortified yogurt product may be 10 wt. % or more. One variety of the protein-fortified yogurt product is a spreadable yogurt product having the total protein concentration of 11 wt. % or more.

Disclosed are liquid nutritional compositions comprising: carbohydrate; lipid having from about 0.1% to about 20% of an n-3 polyunsaturated fatty acid, n-6 polyunsaturated fatty acid, or combinations thereof by weight of the composition; a protein matrix having from about 15% to about 50% of a whey protein fraction by weight of the total protein in the composition; and a limonene-containing material, cranberry oil, or combinations thereof. The compositions are preferably aseptically packaged, and provide enhanced oxidative stability, flavor, and aroma, especially when formulated with relative high polyunsaturated fatty acid concentrations.

The invention relates to an animal origin-free low-protein culture medium suitable for animal cell product production, comprising 24 basic metabolism nutrients, 11 vitamins, 3 transferrin substitute compounds, 5 lipid compounds, 2 nucleic acid compounds, 4 hormones and growth factors, 3 antioxidants, 1 shear-resistant protective agent, 1 pH indicator, 2 pH buffers, 9 other inorganic salts, soy hydrolysates adopted to substitute animal origin component, and composition of ferrous sulfate, ferric nitrate and EDTA-2Na adopted to substitute transferrin. The culture medium can be made by dissolving the aforementioned components in triply distilled water. The positive effects of the culture medium are as follows: the culture medium contains no animal origin component, the total protein content is lower than 10mg/L, which helps separate and purify products and is suitable for production of recombinant protein medicaments; the culture medium supports normal growth and long-term subculturing of animal cells; the culture medium can be used without adaptation, is easily prepared and is suitable for massive production of animal cell products.

A cell culture product is provided for propagating embryonic stem cells, and maintaining their self-renewal and pluripotency characteristics for extended periods of time in culture. The cell culturing, product includes a substrate; and a coating thereon deposited, from a coating solution. The coating solution includes a mixture of extracellular matrix proteins and an aqueous solvent, wherein the total protein concentration in the coating solution is about 10 μg/ml to about 1 mg/ml.

Methods and diagnostic kits for determining whether a subject may develop a or for diagnosing a neurodegenerative disease. The method includes quantitating the amount of alpha-synuclein and total protein in a cerebrospinal fluid (CSF) sample obtained from the subject and calculating a ratio of alpha-synuclein to total protein content; comparing the ratio of alpha-synuclein to total protein content in the CSF sample with the alpha-synuclein to total protein content ratio in CSF samples obtained from healthy neurodegenerative disease-free subjects; and (c) determining from the comparison whether the subject has a likelihood to develop neurodegenerative disease or making a diagnosis of neurodegenerative disease in a subject. A difference in the ratio of alpha-synuclein to total protein content indicates that the subject has a likelihood to develop a neurodegenerative disease or has developed a neurodegenerative disease.

Disclosed are induced viscosity nutritional emulsions comprising (A) protein having a protein-bound methionine sulfoxide content of 8% or less of the total protein-bound methionine, on a molar basis, (B) fat, and (C) an induced viscosity fiber system that provides the emulsion with a packaged viscosity of less than 300 cps and an induced viscosity following consumption of at least 300 cps, wherein the induced viscosity nutritional emulsion is an oil-in-water emulsion. It has been found that product stability is improved and shelf-life increased by selection of those protein sources having a low methionine sulfoxide content.

The invention is directed to seed and seed extract compositions containing levels of a human milk protein between 3-40% or higher of the total protein weight of the soluble protein extractable from the seed. Also disclosed is a method of producing the seed with high levels of extractable human milk protein. The method includes transforming a monocotyledonous plant with a chimeric gene having a protein-coding sequence encoding a protein normally present in human milk under the control of a seed maturation-specific promoter. The method may further includes a leader DNA sequence encoding a monocot seed-specific transit sequence capable to target a linked milk protein to a storage body.

The invention relates to a quantitative detection method for thermal-denaturation and non-denaturation bovine alpha-lactalbumin in milk and milk products by applying an enzymolysis-liquid chromatography and mass spectrometry combination technology. The quantitative detection method comprises the steps as follows: taking a certain amount of milk or milk samples, dissolving and diluting the milk or milk samples in water to obtain solution with total protein content being about 1mg/mL; after volume metering, correctly sucking 500 mu L, adding an internal standard substance, reacting disulfide bond with dithiothreitol (DTT), alkylating to protect sulfydryl produced in reaction by iodoacetamide (IAA), and then conducting constant-temperature and constant-time enzymolysis with trypsin; and separating enzymolysis products by reversed phase liquid chromatography, conducting detection with a mass spectrum multiple reaction monitoring (MRM) manner, and calculating the result by an internal standard method. The quantitation limit of the method is 0.001g/100g; when adding amount is 0.2, 1.7 and 5.0g/100g, the recovery rate is 98.9-110.8% (n is equal to 6) and repeatability: RSD (Relative Standard Deviation) is smaller than 7.6%; and the quantitative detection method can be applicable to the quantitative detection of samples with different contents of bovine alpha-lactalbumin.

The present invention is diphtheria toxin mutant CRM197 and its preparation process, and belongs to the field of immune protein carrier technology. The diphtheria toxin mutant CRM197 is expressed in colibacillus in the form of inclusion body, and the target product has sequence length of 536 amino acids, the immunogenicity the same as that of diphtheria toxin and no toxicity similar to that of diphtheria toxin. The present invention clones gene coding CRM197, constructs recombinant expression plasmid expressing CRM197 and transforms to colibacillus host for expression in the form of inclusion body. The present invention has target protein accounting for over 24 % of total protein content, high CRM197 purity over 95 % and high target protein recovering rate. The preparation process is simple, low in cost and suitable for industrial production.

Disclosed are substantially clear nutritional liquids comprising protein and calcium HMB wherein soluble protein represents from about 65% to 100% by weight of total protein. The liquids have a pH of from about 2.8 to about 4.6 and may be manufactured as a hot fill product. The substantially clear nutritional liquids may also have a weight ratio of calcium HMB to soluble calcium of from 4.5:1 to 7.3:1. In some embodiments, the substantially clear nutritional liquids are substantially free of fat, and may optionally include isomaltulose and/or beta alanine.

Methods for promoting health or wellness, regulating food intake, and increasing nitrogen retention in an adult animal by feeding the animal a food containing lysine in an amount of at least about 3% by weight of total protein. Also, kits containing lysine and a food suitable for consumption in separate packages and instructions for how to combine the lysine and food are provided.

The invention discloses processed cheese and a preparation method thereof. The processed cheese comprises the following materials in percentage by mass: 16 to 26 percent of protein, 20 to 30 percent of fat, 37 to 50 percent of water and 1 to 3 percent of emulsifying salt, wherein complete casein accounts for 75 to 90 mass percent of total protein. The invention provides the processed cheese which can be fried, roasted, or subjected to microwave heating for the first time. After being fried, roasted or subjected to microwave heating, the processed cheese can still keep the external form of a solid phase instead of becoming fluid. The processed cheese is crisp outside and tender inside and delicious after being fried at a high temperature, can completely meet the requirement of frying, roasting or microwave operation, and has wide market prospect.

The invention discloses a process technique for preparing wheat gluten powder peptide by a fermentation and enzymolysis method. A wheat gluten powder active peptide product with relatively high antioxidant activity is obtained by using a microbial fermentation and enzymolysis method, hoping for providing technique support for industrial production of preparing antioxidative peptide by using wheat gluten powder, thereby expanding the application range of the wheat gluten powder and improving the production additional value of the wheat gluten powder. The wheat gluten powder product obtained by adopting ultrasonic-assisted enzymolysis and an aerobic anaerobic two-step fermentation process is white in color and luster, powdery and free from impurities and bitter taste. The total protein content is greater than or equal to 97%, and the content of peptide with the molecular weight which is smaller than 1000 Dalton in total protein is greater than or equal to 98%. The reducing capacity of the wheat gluten powder peptide reaches 0.486 and the ABTS free radical scavenging capacity reaches 1.85mmol/L Trolox. The method disclosed by the invention is short in production period, low in cost and free from generation of any toxic and harmful substances. The obtained product is high in safety and good in functional activity and can be widely applied to the fields such foods, drugs, health products and biological synthesis.

Described herein are a liquid milk beverage for toddlers comprising non-hydrolyzed, non-fermented and protein-rebalanced cow's milk, processes for making same and methods of uses. The non-hydrolyzed, non-fermented rebalanced cow's milk comprises a reduced weight/weight proportion of casein:whey proteins when compared to untreated cow's milk. The non-hydrolyzed, non-fermented rebalanced cow's milk may further comprises a reduced total protein concentration when compared to untreated cow's milk. The reduced proportion and/or reduced total concentration of proteins facilitate digestion of the liquid milk beverage by infant and toddlers. Also described are methods for providing nutrition to a toddler, methods for facilitating transition from breast feeding and/or artificial formula feeding of a child towards drinking of cow's homogenized milk, and methods for increasing digestion of cow's milk in an individual.

The invention discloses a recombinant human collagen and an application thereof. An amino acid sequence of the protein is shown as SEQ ID NO. 3, the nucleotide sequence of the protein-coding gene is shown as SEQ ID NO. 1. The recombinant human collagen of the invention has very good hydrophilicity and stability, and the amino acid composition thereof is 100% identical to the corresponding amino acid sequence of the natural collagen, and the amount of expressed protein can account for about 25% of the total protein of the thalline, per milliliter of the bacteria liquid precipitate contains 0.25mg of the target protein, and the production cost is very low and the cycle is short. The prepared collagen is applied to the human body without immunological rejection and allergic reaction, and canbe widely applied to the biomedicine and cosmetics industries.

A method is disclosed for producing an infant formula base, wherein by means of microfiltration, ultrafiltration, and nanofiltration the components of milk are separated into a casein fraction, a whey protein fraction, and a lactose fraction to produce, by suitably combining, a composition in which the amino acid composition is close to that of human milk. An infant formula base having a total protein concentration of about 1.0 to about 1.5% and a β-casein concentration of at least about 11% of the total protein concentration is also disclosed. The infant formula base is suitable for the production of an infant formula.

The invention relates to a dimensional electrophoresis method for a total protein of a jute root system, belonging to the field of biotechnology. The total protein of the jute root system capable of existing in the coastal beach is separated by the dimensional electrophoresis technique, and by adopting the technical scheme, the jute root system existing in the coastal beach is tested with good effect. At present, repeated experiments prove that the jute root system is separated by the dimensional electrophoresis method to obtain more protein points; and after detected by PDQuest software, the protein points are more than 1100, the map is clear without transverse and longitudinal grains, the protein points are circular, and the repeatability is good, thus the invention is a dimensional electrophoresis method suitable for the analysis of the total proteomics of the jute root system.

The invention relates to the technical field of culture medium development and research of biotechnology and discloses a serum-free medium suitable for large-scale production of influenza vaccines. The serum-free medium comprises 23 basic metabolism nutrients, two nucleic acid compounds, 6 vitamins, 9 inorganic salt compounds, a shear force protective agent, two acidity and alkalinity buffer agents, an acidity and alkalinity indicator, 10 virus reproduction promoters and three additives. The conventional preparation method of the serum-free medium comprises the following steps of: dissolving the components in ultrapure water without a heat source so as to prepare the serum-free medium. The using method is a conventional method. The serum-free medium has the beneficial effects that the serum is not contained, the total protein content is lower than 10mg per liter, separation and purification of the product are promoted, and the serum-free medium is suitable for production of influenza vaccines, supports the normal growth and long-term continuous cell culture of animal cells and can be used without adaption; and moreover, because the components are clear, the serum-free medium is convenient to prepare, controllable in cost and suitable for large-scale production of the influenza vaccines.

The invention relates to a whey protein product having a ratio of whey protein to casein in the range from about 90:10 to about 50:50 and the total protein content of at least 20% on dry matter basis, and a method for its preparation. The product has a favourable amino acid composition and is especially suitable for athletes.

The invention discloses a microbial fermentation enzymolysis method for extracting the grease and protein of soybean, comprising the following steps of: (1) carrying out pretreatment on the soybean; (2) inoculating microbial strains into liquid fermentation culture medium for fermentation and enzyme production; (3) carrying out eccentricity on the fermentation culture medium, and mixing the supernatant liquor with water, and inoculating bean flour for enzymolysis; (4) centrifugating to obtain emulsible liquid, hydrolyzate and residue; and carrying out emulsion breaking and obtaining free oil; and (5) adjusting the pH value of the hydrolyzate, centrifugating, cleaning the precipitate, then centrifugating again, then adjusting the pH value after the precipitate is dissolved by water, drying and obtaining the isolated soy protein. In the microbial fermentation enzymolysis method, all process parameters of the oil and the protein of the soybean by microbial fermentation enzymolysis are studied comprehensively and systematically, the extraction rate of the total oil and the total protein combined with the free oil are adopted as the surveying index on the basis of determining the best culture medium and the culture conditions for fermentation and enzyme production, and the conditions for enzymolysis of soybean flour and emulsion breaking are determined, so that the extraction rate of the total oil in the raw material is up to more than 90%, and the yield of the total protein is up to more than 90%.