752 results about "Protein content" patented technology

The invention relates to the technical field of plant breeding, in particular to a molecular marker related to oil content and protein content of soybean and application of the molecular marker. The molecular marker is constructed on the basis of SNP sites; based on a genome version number Wm82. A2. V1, the SNP site is located at the 8605814th site of the No.8 chromosome of the soybean, and the polymorphism is A / G. The application comprises the following steps: (1) predicting or detecting the oil content or protein content of soybeans; (2) identifying or cultivating soybean varieties with high oil content or protein content; (3) soybean molecular marker assisted breeding; (4) soybean variety improvement related to oil content or protein content; and (5) soybean germplasm resource improvement. The molecular marker related to the soybean oil content and the protein content is obtained through research and screening, and the molecular marker can be applied to cultivation of soybean varieties with high oil content and high protein content and has important value in the field of soybean breeding.

The invention discloses a chicken manure crude protein content detection model construction method, a chicken manure crude protein content detection system and a detection method, relates to the technical field of bioinformatics, solves the problems of high cost, serious pollution and low efficiency of an existing chicken manure crude protein content detection technology, and improves the accuracy of chicken manure resource application. The obtained sample set comprises visible-near infrared reflection spectrum and crude protein content of the chicken manure; carrying out pretreatment on the visible-near infrared reflection spectrum by adopting a plurality of pretreatment methods; screening out an optimal preprocessing method and a corresponding preprocessing spectrum by using a partial least square regression model; obtaining a characteristic wave band by using a plurality of characteristic selection methods; and based on the crude protein content and the characteristic wave band, constructing and screening an optimal chicken manure crude protein content detection model. The method provided by the invention is suitable for detecting the content of crude proteins in chicken manure by using manure resources.

The invention discloses a chicken manure crude protein content detection model construction method, a chicken manure crude protein content detection system and a detection method, relates to the technical field of bioinformatics, and solves the problems that an existing chicken manure crude protein content detection technology is serious in pollution in the detection process, and a spectral analysis technology is serious in information redundancy during detection, high in cost and poor in model stability. The sample set comprises visible-near infrared reflection spectrum and crude protein content of chicken manure; obtaining three spectral indexes corresponding to the visible-near infrared reflection spectrum, the MSC pretreatment spectrum and the SNV pretreatment spectrum, and screening out the optimal wavelength position and the corresponding three spectral indexes; constructing a data set by using the three spectral indexes and the crude protein content, and performing fitting through multiple fitting modes; evaluating the plurality of fitting equations, and screening out an optimal fitting equation and a corresponding pretreatment method and wavelength position as a chicken manure crude protein content detection model. The method provided by the invention is suitable for detecting the content of crude proteins in chicken manure by using manure resources.

The invention provides a fish collagen peptide with skin brightening and freckle removing effects as well as a preparation method and application thereof, and belongs to the technical field of protein peptides. The preparation method comprises the following steps: by taking fish skin and / or fish scales as raw materials, performing degreasing and fishy smell removal treatment, and then sequentially performing heat treatment, homogenization treatment, enzymolysis treatment and enzyme deactivation refining to obtain the fish collagen peptide. In the fish collagen peptide prepared by the invention, the proportion of polypeptide with the molecular weight of 189-3000Da is more than 90%, and the protein content is more than 99.23%. Experiments show that the fish collagen peptide can effectively reduce the skin color spot area proportion of a subject and improve the skin L value and ITA value of the subject at the same time. Therefore, the fish collagen peptide prepared by the invention has good skin brightening and freckle removing effects, and a new way is provided for preparing related products.

The invention discloses enterobacter hormaechei XW-01 and a microbial preparation thereof, the strain (preservation number is CCTCC NO: M 20242595) can efficiently degrade protein, total sugar and nicotine in tobacco leaves, sensory indexes such as aroma and aroma quantity are remarkably improved, and protease activity is remarkably improved when the enterobacter hormaechei XW-01 and wheat bran act synergistically. The microbial preparation is obtained through the processes of strain activation, fermentation culture and microbial agent preparation, and when the microbial preparation is applied to tobacco leaf treatment, through the steps of water content adjustment, preparation spraying, sealed fermentation, low-temperature drying and the like, the technical breakthrough that the protein content is reduced, the tar release amount is reduced, and the comprehensive quality grade is improved is achieved. Compared with a traditional method, the technology has the advantages that the treatment period is shortened, the cost is greatly reduced, the COD value of wastewater is greatly reduced, the cigarette quality and the production environmental protection property are remarkably improved, and the method has a wide industrial application prospect.

The invention discloses a culture method for increasing the protein content of aspergillus niger mycelia by using corn steep liquor as a culture medium, and belongs to the technical field of biological fermentation. Aiming at insufficient resource utilization of corn steep liquor and substitute protein production requirements, a corn processing by-product corn steep liquor is creatively adopted to directly replace a traditional PDB culture medium, and the mycelium protein content is remarkably increased by optimizing a fermentation system. Results show that the corn steep liquor is used as a culture medium, aspergillus niger with the preservation number of CCTCC M 2013703 is inoculated, and aspergillus niger mycelium protein can be obtained through fermentation. The protein content of the mycelium obtained by the process disclosed by the invention reaches 50.62-53.56%, and the total amino acid content is increased by 64-76%. According to the method, high-value conversion of agricultural corn byproducts is realized, and an efficient and low-cost industrial solution is provided for alternative protein production.

The invention discloses saccharomyces cerevisiae MBPSc21 and application thereof, and belongs to the technical field of microorganisms. On one hand, the invention provides a saccharomyces cerevisiae engineering strain MBPSc21 for expressing immune nutrient-beta-carotene, and the preservation number of the saccharomyces cerevisiae engineering strain MBPSc21 is CGMCC No.32663; on the other hand, the invention provides application of the saccharomyces cerevisiae, the strain takes soybean meal as a solid-state fermentation substrate, the crude protein content of the obtained product fermented soybean meal is remarkably increased, anti-nutritional factors such as oligosaccharide and urease are greatly reduced, more importantly, the fermented soybean meal is rich in beta-carotene, and the content of the beta-carotene is reduced. The soybean meal protein is endowed with new functional immune nutrition, so that the overall nutritional value of the soybean meal is improved, and a leading level is achieved in the field of developing functional fermented soybean meal by utilizing saccharomyces cerevisiae.

The invention relates to the technical field of medicine analysis and detection, and particularly discloses a method for detecting the content of protein in a 3D-MLA adjuvant, which comprises the following steps: (1) preparing a test solution: firstly dissolving the 3D-MLA adjuvant by using a hydrochloric acid solution, performing 300-600W ultrasonic treatment for 2-5min, centrifuging 10000-13000g for 4-7min, and taking supernate; adding a compound enzyme into the supernate for enzymolysis; then adding concentrated hydrochloric acid, uniformly mixing, hydrolyzing for 20 + / -2 hours in a nitrogen atmosphere at 105-115 DEG C, blow-drying, and dissolving with a hydrochloric acid solution to obtain a test sample stock solution; adding a derivatization reagent into the test sample stock solution, and carrying out derivatization treatment, extraction and dilution to obtain a test sample solution; and (2) high performance liquid chromatography determination. According to the detection method provided by the invention, the residual quantity of the host cell protein in the 3D-MLA can be rapidly and accurately determined, and the quality control of the 3D-MLA is realized.

The invention relates to the technical field of agricultural waste recycling, and discloses a formula and a preparation method of an amaranthus hypochondriacus and moso bamboo powder microbial fermentation feed, and the materials comprise amaranthus hypochondriacus straw, moso bamboo powder, corn flour, a mineral premix, salt and a microbial fermentation agent. After fermentation, the content of crude fibers is obviously reduced, the content of crude proteins is increased, the effect is optimal according to the proportion of the amaranthus hypochondriacus straws to the moso bamboo powder (5: 5), lignin in the moso bamboo powder is partially degraded, polysaccharide substances are released, the palatability of the feed is improved, the pH value of the amaranthus hypochondriacus straws to the moso bamboo powder (5: 5) in the optimal fermentation group is reduced to 4.2, the content of lactic acid reaches 2.3 g / kg, the total amount of VFA is increased, and the reproduction of harmful bacteria is inhibited; the reasonable proportion of the amaranthus hypochondriacus and the moso bamboo powder can balance carbon and nitrogen sources and promote synergistic fermentation of microorganisms, degradation of crude fibers in the fermentation process is related to acid production of lactic acid bacteria and cellulase secretion of bacillus, and the fermented feed can be used as a coarse feed substitute for ruminants or used in pig feed to improve intestinal health.

The invention provides a pichia pastoris strain for efficiently converting methanol to produce high-methionine-content single-cell protein and application of the pichia pastoris strain, and belongs to the technical field of biology. According to the invention, a strain of pichia pastoris (named as Pichiapastoris CN-SCP 00 with a preservation number of CCTCC (China Center For Type Culture Collection) NO: M2025657) is obtained through separation, and is subjected to multiple rounds of ARTP iterative mutation breeding, so that a strain Pichiapastoris CN-SCPYE421 (with a preservation number of CCTCC NO: M2025658) for efficiently converting methanol to produce single-cell protein containing high methionine is obtained. Compared with a commercial strain, the Pichiapastoris CN-SCPYE421 has higher methanol tolerance and utilization capacity, the fermentation time is remarkably shortened, and in addition, the Pichiapastoris CN-SCPYE421 can well grow at the temperature of 35 DEG C. The Pichiapastoris CN-SCPYE421 is subjected to fermentation culture in a 5L fermentation tank, the dry weight of thalli reaches 120-150g / L, the protein content reaches 65-75% of the dry weight of the thalli, the Pichiapastoris CN-SCPYE421 is rich in various essential amino acids, and the methionine content reaches 2.3%. The invention provides an excellent strain for industrial development of single-cell protein, and has great social significance and economic value for the feed protein industry.

The invention relates to a wheat protein content prediction method and device and a medium, and relates to the technical field of protein content prediction.The wheat protein content prediction method comprises the following steps that near infrared spectrum data of wheat and the corresponding protein content are obtained, and global feature vectors and partition feature vectors of the near infrared spectrum data are extracted; adopting a multi-layer perceptron MLP as a base to construct a wheat protein prediction model; fusing the global feature vector and the partition feature vector to construct a wheat spectral feature vector, and inputting the wheat spectral feature vector and the protein content into a wheat protein prediction model for training to obtain a trained wheat protein prediction model; collecting near infrared spectrum data of wheat to be predicted; and inputting near infrared spectrum data of to-be-predicted wheat into the trained wheat protein prediction model to obtain a protein prediction value of the to-be-predicted wheat. According to the invention, rapid and lossless prediction of the wheat protein content is realized.

The invention discloses a cross-platform soybean seed protein content estimation method based on a spectral time sequence. The method comprises the following steps: step 1, acquiring high spectral data of a soybean canopy; 2, collecting a soybean multispectral image; step 3, acquiring soybean physiological parameters and grain protein content data; step 4, obtaining soybean photosynthetic parameter data; step 5, calculating a vegetation index for estimation; step 6, constructing a vegetation index time sequence; 7, screening an optimal time window; 8, extracting dynamic characteristics; step 9, establishing a model, estimating the soybean protein content, and performing precision verification; and step 10, carrying out mobility analysis on the model from a near-ground canopy to a satellite platform. According to the method, a theoretical basis and a practical path are provided for realizing remote sensing-based soybean GPC accurate monitoring, wide application of the method on a satellite remote sensing platform can be promoted in the future, and high-precision, high-time-efficiency and large-scale soybean GPC estimation is realized.

The invention provides zero-fat low-sugar yoghourt and a preparation method thereof, the zero-fat low-sugar yoghourt comprises the following raw materials: skimmed milk, a fermentation inoculant, a sweetening agent and a protein product, the fat content of the skimmed milk is less than or equal to 0.3 g / 100mL, the protein content of the skimmed milk is greater than or equal to 6.0 g / 100mL, and the lactose content of the skimmed milk is less than or equal to 4.0 g / 100mL; the fermentation inoculant is composed of pichia kudriavzevii, lactobacillus helveticus and streptococcus thermophilus, and the ratio of the three strains is 2: 1: 5. According to the zero-fat low-sugar yoghourt disclosed by the invention, through selection of specific strains, good stability can be realized under the condition that a thickening agent and a stabilizing agent are not added, the product can be endowed with better oxidation resistance, human body metabolism improving efficacy and immunity regulating efficacy, and a green, clean and healthy yoghourt product is really obtained.

The invention discloses a method for producing feed by using corn sugar residues, and belongs to the technical field of fermentation. The method comprises the following steps: pretreating, proportioning, adding an antibacterial agent, fermenting, drying and crushing, coating, cooling and the like, mixing corn sugar residues, corn germ meal and threonine mother liquor according to a specific ratio, adding zinc oxide and a chitosan antibacterial agent, and inoculating Saccharomyces cerevisiae ATCC 9763 for fermenting. And performing multi-stage drying and crushing on the fermentation product, spraying a coating emulsion prepared from a defatted soybean meal extract, curcumin, vitamin E, lecithin and sophorolipid, and performing air cooling to prepare the feed. According to the method, by-products are fully utilized, the crude protein content of the feed is 32-34.5%, the diameter of an inhibition zone is 18.1-20.2 mm, the moisture absorption rate is 2.7-3.4%, the palatability score is 8.0-8.6, and the fermentation yield is 92-94%. The innovation point lies in specific strain fermentation and functional coating, and is suitable for industrial production.

The invention provides a spleen peptide for promoting growth and tonifying spleen as well as a preparation method and application thereof, and belongs to the technical field of biochemistry. The invention provides a preparation method of spleen peptide capable of promoting growth and tonifying spleen. The preparation method comprises the following steps: degreasing spleen particles of healthy animals, fermenting after degreasing, performing subcritical water extraction, performing protease enzymolysis, fermenting an enzymolysis product, clarifying fermentation liquor, and then performing filtration, nanofiltration and spray drying treatment. According to the preparation method, the preparation process of the spleen peptide is reasonably set, so that the prepared spleen peptide is high in protein content, low in average molecular weight, easy to absorb and utilize, free of fat detection, capable of tonifying the spleen and promoting growth, capable of being dissolved into water to form transparent liquid, capable of being processed into tablets, powder and oral liquid, also capable of being used in common food, high in yield and suitable for popularization and application. The method is suitable for industrial popularization.

The invention discloses a beef cattle straw feed preparation method based on compound strain synergistic fermentation, and relates to the technical field of feed processing, and the beef cattle straw feed preparation method comprises the following steps: screening bacillus, lactic acid bacteria and the like to construct an initial compound strain, and screening a high-degradation-ability strain combination through a directed evolution system (30-45 DEG C, pH 4.0-7.0, and lignocellulose 5-15g / L); the straw lignocellulose structure is destroyed through microwave-assisted pretreatment (the power is 200-800 W, and the time is 1-5 min); high-efficiency fermentation is realized through double-stage fermentation (the pH is reduced at 30-35 DEG C for 12-24 hours in the first stage, and stubborn components are degraded at 35-40 DEG C for 36-60 hours in the second stage) and intelligent regulation and control. According to the method, the content of crude protein in the straw is increased to 15.2%, the content of crude fibers is reduced to 19.8%, the degradation rate of lignocellulose reaches 62%, the quality of the feed is remarkably improved, and a new path is provided for recycling of agricultural wastes.

The invention discloses bacillus subtilis, a method for preparing yellow corn silage feed under a strong alkali condition and a product thereof, and belongs to the technical field of microorganisms. The technical problem to be solved is that the survival rate of the existing bacillus subtilis is lower than 30% under the strong alkali condition of pH 9-11, and the existing bacillus subtilis cannot normally grow and propagate. The preparation of the yellow corn silage feed under a strong alkali condition needs additional pH adjustment, the process is complex and the cost is high; the crude protein content of the existing yellow corn silage feed is only 3-5%, and the requirements of livestock cannot be met. According to the technical scheme, the strong-alkali-resistant bacillus subtilis is provided, the preservation number is CGMCC No.36677, the survival rate of the strain is larger than or equal to 80% under the condition that the pH is 9-11, the straw degradation rate is larger than or equal to 25%, no waste water is generated when the strain is used for preparing the yellow silage feed, the cost is reduced compared with an existing technology, the protein content is increased by 50%-80%, palatability is good, and the method is suitable for large-scale straw resource utilization and feed protein production.

The invention relates to the technical field of animal nutrition information modeling, in particular to a chicken nutrition modeling and visual analysis system which comprises a nutrition requirement evaluation module, a proportion optimization calculation module, a nutrition trend analysis module, a proportion adjustment module and a nutrition diagnosis decision module. According to the method, accurate description of a nutritional demand structure is realized through the weight growth rate and the change trend of the ingestion behavior, efficient matching of feed energy and protein content is assisted by extracting a comparison result of absorption fluctuation and demand intensity, a high-frequency demand stage is identified according to the time sequence characteristics of the weight fluctuation, and dynamic tracking of a nutritional demand period is realized. According to the method, the feed matching value is revised in a segmented mode according to the ingestion time delay and the absorption difference, so that the matching response is close to the growth rhythm, the nutrition attribution path is recognized through the absorption difference and the fluctuation rule, the nutrition supply adaptability and the ingredient pertinence are improved, the feed waste rate and the nutrition imbalance probability are reduced, and the feeding benefit and the management intelligent level are improved.

The invention relates to a waste vinasse drying treatment method. The method comprises the following steps: carrying out mechanical pressure filtration on original waste vinasse provided in batches in a solid-liquid separation manner; dehydrating the waste distiller's grains in a manner of removing moisture in the waste distiller's grains and gas influencing the caking rate; and screening the high-fiber waste vinasse and the heated and dehydrated waste vinasse separated from the organic matter-enriched waste vinasse based on a protein content differential separation mode to obtain oversize products for pyrolysis and undersize products for feed. According to the method, more moisture is reserved in the waste distiller's grains with high caking rate in mechanical filter pressing, the water in the waste distiller's grains can enable the waste distiller's grains to be agglomerated more easily, meanwhile, the high heat capacity characteristic of water can provide more uniform heat conduction for the waste distiller's grains, and the situation that the internal and external dehydration uniformity is not uniform due to local overheating of the waste distiller's grains is avoided.

The present application provides a kind of beverage with chickpea as plant protein source and a preparation method thereof.The protein in the prepared chickpea protein beverage is 100% or mainly derived from natural chickpea seeds.No stabilizer, thickening agent and sweetener is added in the product, and the above-mentioned purposes are achieved only by physical processing, enzyme hydrolysis and pH adjustment.The chickpea protein and starch components can be fully utilized, the fullness of the plant protein beverage is improved, and the chickpea protein beverage can still meet the commercial sterile requirements even under the condition of higher protein content.

The invention discloses high-protein instant noodles and a preparation method thereof, the high-protein instant noodles comprise soybean protein isolate, and the content of the soybean protein isolate accounts for 5wt%-30wt% of the total mass of raw material powder. According to the high-protein instant noodles provided by the invention, the formula of the instant noodles is optimized, the soybean protein isolate which accounts for 5-30wt% of the total mass of the raw material powder is added, and the soybean protein isolate is modified, so that the soybean protein isolate is more suitable for being applied to the instant noodles, the protein content of the instant noodles is increased, and the instant noodles are better in mouth feel; the problems that existing instant noodles are limited in nutritional value, relatively low in protein content, prone to collapsing after being cooked and the like are solved.

The invention discloses an Indel marker related to a corn kernel protein content character and application of the Indel marker, and belongs to the technical field of plant molecular genetics and crop molecular design breeding. The Indel marker is located on a gdh2 gene of a chromosome 10 of a corn genome, and insertion or deletion of a nucleotide sequence as shown in SEQ ID NO.5 occurs in an intron region between a second exon and a third exon. By applying the molecular marker-assisted selection technology, high-protein new germplasms S3317 (the crude protein content is 17.0%) and JS231148 (the crude protein content is 15.6%) have been successfully cultivated, the increase amplitudes of the high-protein new germplasms and JS231148 are both more than 15% compared with those of the conventional control, and the lysine content reaches 0.41% and reaches the national high-quality protein corn standard.

The invention relates to the field of plant growth regulators, in particular to a conditioner group for improving the grain quality and the rice quality of direct seeding rice and a preparation and use method thereof.The preparation method of a conditioner in the initial heading stage comprises the following steps that 1, 6-benayl aminopurine is dissolved in a diluted hydrochloric acid solution in a assisted mode, and a 6-benayl aminopurine solution is obtained; step 2, adding choline chloride into the solution obtained in the step 1, stirring to obtain a mixed solution, transferring the mixed solution into a volumetric flask, and adjusting the pH value; 3, abscisic acid powder is dissolved with absolute ethyl alcohol, volume metering is carried out after dissolution to form a stock solution, and then PH adjustment is carried out. Two conditioners are included and sprayed in the initial heading stage and the full heading stage respectively, glumous flower degeneration can be reduced, the photosynthetic production capacity of the upper three leaves after flowering can be improved, assimilation substances are promoted to be transferred to grains, especially the filling state of vulnerable grains is improved, and the yield of the seedlings is increased. Finally, the grain number, the maturing rate, the grain weight and the yield are improved, meanwhile, the head rice rate of rice can be improved, and the protein content is reduced, so that the palatability of rice is improved.

The present invention relates to the technical field of bacterial enzyme fermentation, and provides a bacterial enzyme synergistic fermentation method for removing antinutritional factors in soybean meal, the method comprises the following steps: S1, soybean meal pretreatment: soybean meal, water and an auxiliary agent are mixed and then subjected to steam explosion treatment, and the auxiliary agent comprises citric acid, a hydrogen peroxide solution and polyphenol; s2, bacterial enzyme fermentation: mixing the pretreated soybean meal with molasses and water to obtain a raw material, adding a complex microbial inoculant and a complex enzyme, uniformly stirring, and fermenting; the complex microbial inoculants comprise saccharomycetes, bacillus subtilis, lactobacillus rhamnosus and pediococcus pentosaceus; the compound enzyme comprises acid protease, pectinase, phytase, glucanase, alpha-galactosidase and keratinase; and S3, after fermentation is finished, drying the materials to obtain the fermented soybean meal. According to the fermentation method, the glycinin degradation rate can reach 79% or above, the acid soluble protein content is increased to 26% or above, the anti-nutritional factor content is remarkably reduced, and the nutritional value is greatly increased.

The invention discloses a processing method and application of edible fungus chaff. The processing method comprises the following steps: crushing the edible fungus chaff to obtain fungus chaff particles; carrying out extrusion puffing treatment on the mushroom bran particles by adopting a twin-screw extruder to obtain a puffed material; drying and crushing the puffed material to obtain puffed mushroom bran powder; mixing puffed mushroom bran powder, bean dregs and cellulase to serve as a fermentation substrate, and adding the mixed bacterial liquid into the fermentation substrate to form fermentation mixed liquid; the mixed bacterial liquid is formed by mixing a candida utilis bacterial liquid, a lactobacillus plantarum bacterial liquid and a bacillus subtilis bacterial liquid; and fermenting the fermentation mixed solution under a ventilation condition to obtain a final edible mushroom bran product. Compared with a traditional processing method, the content of crude protein, the content of acid-soluble protein and the protein dispersion index of the edible fungus chaff processed through the processing method are all remarkably improved, and the content of crude fiber, neutral detergent fiber and acid detergent fiber is all remarkably reduced.

The invention discloses instant high-protein food formed by compounding and extruding soybean protein and cereals and a preparation process of the instant high-protein food, and relates to the field of instant food, the instant high-protein food is prepared by adopting soybean protein powder, cereal powder and water as raw materials and performing extrusion puffing, and the raw materials comprise, by weight, 30-60 parts of soybean protein powder, 20-30 parts of cereal powder and 20-30 parts of water. 40 to 70 parts of cereal powder; the cereal flour is selected from at least one of oat flour, quinoa flour, coarse rice flour and whole wheat flour; the water accounts for 15-35% of the total dry weight of the raw materials; wherein the protein content (dry basis) is greater than or equal to 30%; the puffing density is 0.3-0.5 g / cm < 3 >; according to the invention, the raw materials are accurately selected, the high-quality vegetable protein is adopted, the cereal powder is reasonably matched, and the characteristic that the content of beta-glucan in the oat powder is not less than 4% is utilized, so that a continuous gel network structure is formed in the extrusion process; and the inhibition effect of a compact network formed by a vegetable protein molecular chain during extrusion expansion on gelatinization expansion of cereal starch is effectively relieved.

The invention provides a method for improving forage grass production performance and silage quality and application of the method, and belongs to the technical field of silage production. The method comprises the following steps that after silage corn is sown, lacroton beans are sown between two rows of corn immediately; first topdressing is carried out 18-22 days after sowing; carrying out secondary topdressing 60-68 days after sowing; harvesting is conducted on the 105th-110th day after sowing is conducted; and carrying out silage on the harvested silage corn and the harvested lacroton beans. Through mixed sowing of the corn and the lab beans, the dry matter yield and the crude protein content of the mixed forage grass are improved, supply of protein raw materials in concentrated feed is reduced, and the feed cost is reduced. The abundance of leuconostoc can be remarkably reduced during the ensiling period, and the silage obtained after ensiling is excellent in sensory evaluation. The content of nutritional ingredients in the silage is changed, protein and carbohydrate components are changed, and the nutritional value of the silage is improved.

The invention relates to the technical field of food quality detection, and discloses a meat product comprehensive scoring method based on principal component analysis, and the method comprises the steps: firstly obtaining physicochemical index (such as protein and fat content) and sensory attribute (such as color saturation and elasticity index) data of multiple batches of samples, and carrying out the structural arrangement of the two types of data according to batches and index dimensions; respectively carrying out Z-score standardization, range normalization and other processing, and extracting a physicochemical index feature vector and a sensory attribute feature vector; constructing a dynamic weight strategy through a variance contribution rate and a feature root ratio, and performing weighted fusion on the two types of feature vectors to obtain a fused feature vector; and finally, inputting the data into a scoring model, and after centesimal system conversion and distribution calibration, dividing quality grades in combination with an industry standard threshold to generate a comprehensive scoring result containing alphanumeric identifiers. The method realizes multi-dimensional data fusion and dynamic empowerment, improves the scoring accuracy, and is suitable for meat product quality evaluation.

The invention discloses a biological energy feed fermentation yeast based on enzyme-mineral synergistic activation and a preparation method thereof. According to the scheme, dual functions of mineral activation and microbial conversion are focused, clinoptilolite, sodium bentonite and kaolin are taken as mineral carriers, lignite humic acid is taken as a nutrient synergist, brown sugar, rice bran and bran organic matrixes are matched, and mineral lattice structures are broken through mechanical crushing and biological enzymolysis pretreatment, so that the mineral active substances are obtained. And through a double-stage fermentation process of'aerobic fermentation to activate flora and anaerobic aging to enrich metabolites' of microorganisms, mineral ions, humic acid active ingredients and probiotic metabolites are released to the maximum extent, and finally, the high-quality biological energy feed fermentation yeast with nutrition supply, digestion promotion and immune regulation functions is formed. According to the bioenergy feed prepared by conversion of the fermentation yeast, the feed crude protein improvement rate is greater than or equal to 20%, the crude fiber degradation rate is greater than or equal to 40%, the feed conversion rate is improved by 10-15%, the intestinal disease incidence rate is reduced by 60-70%, and the bioenergy feed is suitable for various livestock and poultry and aquaculture scenes.

The invention discloses an enzyme-linked immuno sorbent assay (ELISA) technology-based detection optimization method for A2 protein content in milk, and relates to the technical field of protein content detection.The method comprises the following steps: collecting a milk matrix interference detection data set, and establishing a milk matrix interference corrector; generating an ELISA dynamic detection module; preparing a milk sample to be detected, and controlling the ELISA dynamic detection module to perform protein optimization detection on the milk sample to be detected to obtain milk absorbance detection data; and establishing an absorbance-concentration standard curve, and determining the detection result of the A2 protein content of the milk. The technical problems that in the prior art, when the A2 protein in milk is detected through ELISA, the detection accuracy and reliability are poor due to the fact that the A2 protein in the milk is easily interfered by components and the adaptability to different types of milk is insufficient are solved, the A2 protein content in the milk can be detected more accurately, the anti-interference capacity is higher, and the detection accuracy and reliability are improved. The technical effect of improving the accuracy and the reliability of the ELISA technology for detecting the A2 protein of the milk is achieved.