1027 results about "Protein content" patented technology

The invention discloses a method and a system for efficiently and rapidly detecting protein content and moisture content in a plant-based product based on near infrared spectrum and deep learning, and the method comprises the following steps: obtaining spectral data of a plant-based sample, and carrying out experimental determination on the protein content and the moisture content; in combination with multi-step data preprocessing and adaptive dimensionality reduction, feature signals highly related to target detection are extracted, and a deep learning model based on a convolutional neural network and a self-attention mechanism is utilized to construct a high-precision detection model; a final stable detection model is obtained through multiple times of verification and optimization of the model, and hardware integration is carried out on the final stable detection model and a portable spectrometer to form a detection system. The detection system comprises a 3D printing shell, a spectrum sensor, a conversion line and a data processing unit, the protein content and the water content of a sample can be rapidly and nondestructively detected through one-key operation, and the whole process does not need chemical reagent intervention. The method has the advantages of being high in detection speed, high in portability, easy and convenient to operate, wide in application scene and the like, and is particularly suitable for application occasions needing efficient and on-site real-time detection.

The invention relates to a culture method for improving duckweed protein, which comprises the following steps: purifying duckweed in a Hoagland working solution to obtain purified duckweed; mixing the biogas slurry with the Hoagland working solution to prepare a duckweed culture solution; wherein the concentration of the biogas slurry is 1.5%-5%, and the concentration of the Hoagland working solution is 95%-98.5%; after the purified duckweed is put into the duckweed culture solution, the duckweed culture solution is put into an incubator with preset culture conditions to be cultured, and the cultured duckweed is obtained. By adding the biogas slurry into the Hoagland working solution, the protein content of the cultured duckweed can be remarkably increased, the biogas slurry can be efficiently utilized, water resources are saved, an ideal way is provided for wastewater recycling, meanwhile, nitrogen and phosphorus in the biogas slurry can be utilized for promoting synthesis of duckweed protein, the protein synthesis efficiency is improved, and the yield of duckweed is increased. And a good basis is provided for feed research of duckweed.

The invention discloses a Trichotoma sp. ZSL-NS2 strain capable of producing protein and application of the Trichotoma sp. ZSL-NS2 strain in wastewater treatment of a dairy farm. The Trichotoma sp. ZSL-NS2 strain named as the Trichotoma sp. ZSL-NS2 strain is preserved in the China Center for Type Culture Collection on November 7, 2024, and the preservation number is CCTCC NO.M20242469. The Trichotoma sp. ZSL-NS2 strain is named as the Trichotoma sp. ZSL-NS2 strain. The algal strain is separated from soil beside a wastewater storage pool of a dairy farm, is purified and enriched under a high-salt condition (the salinity is 2.0-5.0%), has relatively strong wastewater tolerance and pollutant purification capacity, has the removal rates of more than 70% of COD, 85% of ammonia nitrogen, 75% of TN and 95% of conventional pollutants respectively, and particularly has the most obvious removal effect on ammonia nitrogen and total phosphorus. In addition, the protein content of the conneronea is as high as 52%, and the conneronea can be used as a protein raw material for high-value application.

The invention relates to the technical field of plant breeding, in particular to a molecular marker related to oil content and protein content of soybean and application of the molecular marker. The molecular marker is constructed on the basis of SNP sites; based on a genome version number Wm82. A2. V1, the SNP site is located at the 8605814th site of the No.8 chromosome of the soybean, and the polymorphism is A / G. The application comprises the following steps: (1) predicting or detecting the oil content or protein content of soybeans; (2) identifying or cultivating soybean varieties with high oil content or protein content; (3) soybean molecular marker assisted breeding; (4) soybean variety improvement related to oil content or protein content; and (5) soybean germplasm resource improvement. The molecular marker related to the soybean oil content and the protein content is obtained through research and screening, and the molecular marker can be applied to cultivation of soybean varieties with high oil content and high protein content and has important value in the field of soybean breeding.

The invention relates to the technical field of genetic engineering, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker related to the protein content of wheat grains and application. The SNP molecular marker contains a nucleotide sequence of which the polymorphism is T or C at the 167440648 bp position of a wheat 7B chromosome. According to the present invention, the research determines that the site is the SNP site closely related to the protein content, and the specific KASP primer combination designed based on the SNP site can rapidly and accurately identify the wheat protein content. According to the technical scheme provided by the invention, the selection efficiency can be improved, the breeding period can be shortened, and wheat varieties with ideal protein content can be quickly identified and cultivated. The KASP primer combination provided by the invention provides an effective tool for molecular marker-assisted selection of wheat nutritional quality related traits.

The invention provides a near infrared spectrum rapid evaluation method for the protein content of brown rice. The method comprises the following steps: acquiring optical signals of different varieties of brown rice under a near-infrared band and physicochemical indexes measured according to a traditional method, preprocessing the acquired optical signals by utilizing a smoothing (Savitzky-Golay) method, and constructing a brown rice protein content evaluation model based on machine learning and a characteristic band selection algorithm; effective prediction of the protein content of the brown rice is realized only through optical characteristic parameters. According to the method, the protein content of the brown rice can be predicted only by collecting the near infrared spectrum of the brown rice, and in food processing enterprises, the technology can remarkably improve the efficiency and stability of product quality monitoring, support real-time online detection and provide an efficient and rapid quality control scheme for the enterprises, so that the product consistency is improved, the waste of raw materials is reduced, and the production cost is reduced. The production benefit is improved.

The invention discloses a chicken manure crude protein content detection model construction method, a chicken manure crude protein content detection system and a detection method, relates to the technical field of bioinformatics, solves the problems of high cost, serious pollution and low efficiency of an existing chicken manure crude protein content detection technology, and improves the accuracy of chicken manure resource application. The obtained sample set comprises visible-near infrared reflection spectrum and crude protein content of the chicken manure; carrying out pretreatment on the visible-near infrared reflection spectrum by adopting a plurality of pretreatment methods; screening out an optimal preprocessing method and a corresponding preprocessing spectrum by using a partial least square regression model; obtaining a characteristic wave band by using a plurality of characteristic selection methods; and based on the crude protein content and the characteristic wave band, constructing and screening an optimal chicken manure crude protein content detection model. The method provided by the invention is suitable for detecting the content of crude proteins in chicken manure by using manure resources.

The invention discloses a chicken manure crude protein content detection model construction method, a chicken manure crude protein content detection system and a detection method, relates to the technical field of bioinformatics, and solves the problems that an existing chicken manure crude protein content detection technology is serious in pollution in the detection process, and a spectral analysis technology is serious in information redundancy during detection, high in cost and poor in model stability. The sample set comprises visible-near infrared reflection spectrum and crude protein content of chicken manure; obtaining three spectral indexes corresponding to the visible-near infrared reflection spectrum, the MSC pretreatment spectrum and the SNV pretreatment spectrum, and screening out the optimal wavelength position and the corresponding three spectral indexes; constructing a data set by using the three spectral indexes and the crude protein content, and performing fitting through multiple fitting modes; evaluating the plurality of fitting equations, and screening out an optimal fitting equation and a corresponding pretreatment method and wavelength position as a chicken manure crude protein content detection model. The method provided by the invention is suitable for detecting the content of crude proteins in chicken manure by using manure resources.

The present disclosure provides a method of purifying or preparing microbial protein concentrate from microbial cell mass. More particularly, the present disclosure provides a method of preparing a microbial protein concentrate, comprising: subjecting a microbial cell mass to a temperature of about 100°C to 210°C and a pressure of about 0.1 bar to 20 bar to obtain a treated microbial cell mass; and separating protein from the treated microbial cell mass to obtain microbial protein concentrate with reduced nucleic acid content and color & odor removed. Additionally, the microbial protein concentrate obtained by the present method has high crude protein content and improved functional properties.

The invention provides a fish collagen peptide with skin brightening and freckle removing effects as well as a preparation method and application thereof, and belongs to the technical field of protein peptides. The preparation method comprises the following steps: by taking fish skin and / or fish scales as raw materials, performing degreasing and fishy smell removal treatment, and then sequentially performing heat treatment, homogenization treatment, enzymolysis treatment and enzyme deactivation refining to obtain the fish collagen peptide. In the fish collagen peptide prepared by the invention, the proportion of polypeptide with the molecular weight of 189-3000Da is more than 90%, and the protein content is more than 99.23%. Experiments show that the fish collagen peptide can effectively reduce the skin color spot area proportion of a subject and improve the skin L value and ITA value of the subject at the same time. Therefore, the fish collagen peptide prepared by the invention has good skin brightening and freckle removing effects, and a new way is provided for preparing related products.

The invention discloses enterobacter hormaechei XW-01 and a microbial preparation thereof, the strain (preservation number is CCTCC NO: M 20242595) can efficiently degrade protein, total sugar and nicotine in tobacco leaves, sensory indexes such as aroma and aroma quantity are remarkably improved, and protease activity is remarkably improved when the enterobacter hormaechei XW-01 and wheat bran act synergistically. The microbial preparation is obtained through the processes of strain activation, fermentation culture and microbial agent preparation, and when the microbial preparation is applied to tobacco leaf treatment, through the steps of water content adjustment, preparation spraying, sealed fermentation, low-temperature drying and the like, the technical breakthrough that the protein content is reduced, the tar release amount is reduced, and the comprehensive quality grade is improved is achieved. Compared with a traditional method, the technology has the advantages that the treatment period is shortened, the cost is greatly reduced, the COD value of wastewater is greatly reduced, the cigarette quality and the production environmental protection property are remarkably improved, and the method has a wide industrial application prospect.

The invention discloses a method for removing fishy smell of minced fillet and improving gel characteristics based on an electric field effect and application of the method, and relates to the technical field of production of minced fillet products. Comprising the following steps: treating surimi rinsing wastewater by utilizing a low-voltage direct-current electric field to obtain rinsing recovery liquid; rinsing the fresh surimi by using the rinsing recovery liquid, and introducing nitrogen microbubbles in the rinsing process to make full contact with the surimi; and rinsing with clear water, and dehydrating to finish the treatment. According to the method disclosed by the invention, the rinsing recovery liquid treated by the electric field is selected and has a synergistic effect with the introduced nitrogen microbubbles, so that the myofibrillar protein content and the moisture content of the rinsed minced fillet are relatively high, and the fat content and fishy smell substances are obviously reduced; the gel strength of the final gel product reaches 493.03 g.cm and meets the 3A-grade surimi standard (the gel strength is greater than or equal to 400 g.cm), and the quality, color and flavor of the surimi gel are effectively improved; the method is simple to operate, high in efficiency and suitable for industrial production.

The invention discloses a culture method for increasing the protein content of aspergillus niger mycelia by using corn steep liquor as a culture medium, and belongs to the technical field of biological fermentation. Aiming at insufficient resource utilization of corn steep liquor and substitute protein production requirements, a corn processing by-product corn steep liquor is creatively adopted to directly replace a traditional PDB culture medium, and the mycelium protein content is remarkably increased by optimizing a fermentation system. Results show that the corn steep liquor is used as a culture medium, aspergillus niger with the preservation number of CCTCC M 2013703 is inoculated, and aspergillus niger mycelium protein can be obtained through fermentation. The protein content of the mycelium obtained by the process disclosed by the invention reaches 50.62-53.56%, and the total amino acid content is increased by 64-76%. According to the method, high-value conversion of agricultural corn byproducts is realized, and an efficient and low-cost industrial solution is provided for alternative protein production.

The invention discloses saccharomyces cerevisiae MBPSc21 and application thereof, and belongs to the technical field of microorganisms. On one hand, the invention provides a saccharomyces cerevisiae engineering strain MBPSc21 for expressing immune nutrient-beta-carotene, and the preservation number of the saccharomyces cerevisiae engineering strain MBPSc21 is CGMCC No.32663; on the other hand, the invention provides application of the saccharomyces cerevisiae, the strain takes soybean meal as a solid-state fermentation substrate, the crude protein content of the obtained product fermented soybean meal is remarkably increased, anti-nutritional factors such as oligosaccharide and urease are greatly reduced, more importantly, the fermented soybean meal is rich in beta-carotene, and the content of the beta-carotene is reduced. The soybean meal protein is endowed with new functional immune nutrition, so that the overall nutritional value of the soybean meal is improved, and a leading level is achieved in the field of developing functional fermented soybean meal by utilizing saccharomyces cerevisiae.

The invention relates to the technical field of medicine analysis and detection, and particularly discloses a method for detecting the content of protein in a 3D-MLA adjuvant, which comprises the following steps: (1) preparing a test solution: firstly dissolving the 3D-MLA adjuvant by using a hydrochloric acid solution, performing 300-600W ultrasonic treatment for 2-5min, centrifuging 10000-13000g for 4-7min, and taking supernate; adding a compound enzyme into the supernate for enzymolysis; then adding concentrated hydrochloric acid, uniformly mixing, hydrolyzing for 20 + / -2 hours in a nitrogen atmosphere at 105-115 DEG C, blow-drying, and dissolving with a hydrochloric acid solution to obtain a test sample stock solution; adding a derivatization reagent into the test sample stock solution, and carrying out derivatization treatment, extraction and dilution to obtain a test sample solution; and (2) high performance liquid chromatography determination. According to the detection method provided by the invention, the residual quantity of the host cell protein in the 3D-MLA can be rapidly and accurately determined, and the quality control of the 3D-MLA is realized.

The invention provides a high-throughput fruit fly food intake measuring method and application thereof, and belongs to the field of animal food intake research. The method comprises the following steps: correspondingly transferring each of a plurality of fruit flies to be detected into each hole of a feeding plate of a high-throughput fruit fly feeding platform; respectively adding liquid food with set chemical components into each hole of a food bottom plate of the high-flux fruit fly feeding platform; enabling a plurality of to-be-detected fruit flies to freely ingest at a set time; measuring the content of protein in the residual liquid food after free ingestion to obtain the content of residual protein in each hole; and obtaining the food intake of each corresponding fruit fly to be detected according to the residual protein content in each hole. Based on the combination of a high-throughput fruit fly feeding platform and protein concentration measurement, the change of the liquid volume is calculated by tracking the change of the content of protein in liquid food, so that the fruit fly food intake measurement efficiency and accuracy are improved while the fruit fly food intake measurement is performed in a high-throughput manner.

The invention relates to the technical field of agricultural waste recycling, and discloses a formula and a preparation method of an amaranthus hypochondriacus and moso bamboo powder microbial fermentation feed, and the materials comprise amaranthus hypochondriacus straw, moso bamboo powder, corn flour, a mineral premix, salt and a microbial fermentation agent. After fermentation, the content of crude fibers is obviously reduced, the content of crude proteins is increased, the effect is optimal according to the proportion of the amaranthus hypochondriacus straws to the moso bamboo powder (5: 5), lignin in the moso bamboo powder is partially degraded, polysaccharide substances are released, the palatability of the feed is improved, the pH value of the amaranthus hypochondriacus straws to the moso bamboo powder (5: 5) in the optimal fermentation group is reduced to 4.2, the content of lactic acid reaches 2.3 g / kg, the total amount of VFA is increased, and the reproduction of harmful bacteria is inhibited; the reasonable proportion of the amaranthus hypochondriacus and the moso bamboo powder can balance carbon and nitrogen sources and promote synergistic fermentation of microorganisms, degradation of crude fibers in the fermentation process is related to acid production of lactic acid bacteria and cellulase secretion of bacillus, and the fermented feed can be used as a coarse feed substitute for ruminants or used in pig feed to improve intestinal health.

The invention provides a pichia pastoris strain for efficiently converting methanol to produce high-methionine-content single-cell protein and application of the pichia pastoris strain, and belongs to the technical field of biology. According to the invention, a strain of pichia pastoris (named as Pichiapastoris CN-SCP 00 with a preservation number of CCTCC (China Center For Type Culture Collection) NO: M2025657) is obtained through separation, and is subjected to multiple rounds of ARTP iterative mutation breeding, so that a strain Pichiapastoris CN-SCPYE421 (with a preservation number of CCTCC NO: M2025658) for efficiently converting methanol to produce single-cell protein containing high methionine is obtained. Compared with a commercial strain, the Pichiapastoris CN-SCPYE421 has higher methanol tolerance and utilization capacity, the fermentation time is remarkably shortened, and in addition, the Pichiapastoris CN-SCPYE421 can well grow at the temperature of 35 DEG C. The Pichiapastoris CN-SCPYE421 is subjected to fermentation culture in a 5L fermentation tank, the dry weight of thalli reaches 120-150g / L, the protein content reaches 65-75% of the dry weight of the thalli, the Pichiapastoris CN-SCPYE421 is rich in various essential amino acids, and the methionine content reaches 2.3%. The invention provides an excellent strain for industrial development of single-cell protein, and has great social significance and economic value for the feed protein industry.

The invention relates to a wheat protein content prediction method and device and a medium, and relates to the technical field of protein content prediction.The wheat protein content prediction method comprises the following steps that near infrared spectrum data of wheat and the corresponding protein content are obtained, and global feature vectors and partition feature vectors of the near infrared spectrum data are extracted; adopting a multi-layer perceptron MLP as a base to construct a wheat protein prediction model; fusing the global feature vector and the partition feature vector to construct a wheat spectral feature vector, and inputting the wheat spectral feature vector and the protein content into a wheat protein prediction model for training to obtain a trained wheat protein prediction model; collecting near infrared spectrum data of wheat to be predicted; and inputting near infrared spectrum data of to-be-predicted wheat into the trained wheat protein prediction model to obtain a protein prediction value of the to-be-predicted wheat. According to the invention, rapid and lossless prediction of the wheat protein content is realized.

The invention discloses a lightweight model-based rapeseed protein and grease content online detection method, device and system, and belongs to the technical field of analysis and detection. A data set covering images of rapeseeds in Zhong China, South China and East China is constructed, and a rapeseed protein and grease content prediction model is developed based on a convolutional neural network. According to the method, a FasterNet model is selected and used for predicting the protein content and the grease content of rapeseeds, and the FasterNet model is deployed in a mobile terminal. According to the method, nondestructive and rapid prediction of the protein and the oil content of the rapeseed is realized, the mobile terminal supports the user to upload the rapeseed image anytime and anywhere, and the prediction result is displayed in a visual manner, so that real-time prediction and evaluation of the protein and the oil content of the rapeseed are realized.

The invention discloses a cross-platform soybean seed protein content estimation method based on a spectral time sequence. The method comprises the following steps: step 1, acquiring high spectral data of a soybean canopy; 2, collecting a soybean multispectral image; step 3, acquiring soybean physiological parameters and grain protein content data; step 4, obtaining soybean photosynthetic parameter data; step 5, calculating a vegetation index for estimation; step 6, constructing a vegetation index time sequence; 7, screening an optimal time window; 8, extracting dynamic characteristics; step 9, establishing a model, estimating the soybean protein content, and performing precision verification; and step 10, carrying out mobility analysis on the model from a near-ground canopy to a satellite platform. According to the method, a theoretical basis and a practical path are provided for realizing remote sensing-based soybean GPC accurate monitoring, wide application of the method on a satellite remote sensing platform can be promoted in the future, and high-precision, high-time-efficiency and large-scale soybean GPC estimation is realized.

The invention relates to the technical field of quality control, in particular to a raw material quality intelligent screening optimization system, which comprises a preliminary feature screening module, a raw material detection database, a sample humidity, protein content and fat proportion data extraction module, and a mean value and a standard deviation of each item of data calculation module. According to the method, the humidity, the protein content and the fat proportion of the raw materials are finely screened and analyzed, the key features influencing the product quality are identified, the feature weight is dynamically adjusted, and real-time optimization is performed according to the performance and the error of the features in prediction, so that the reaction speed and the accuracy of the model are improved; abnormal data points in a data set are cleared, data quality is optimized, processing efficiency and prediction accuracy are further improved, features are adjusted and recombined through real-time production data, feature configuration is made to better meet actual production requirements, prediction stability and accuracy are enhanced through a multi-model fusion technology, and prediction efficiency is improved. Therefore, the overall production efficiency and the product quality are improved.

The invention belongs to the technical field of molecular biology, and particularly relates to a molecular marker related to the content of crude protein of medicago sativa and application of the molecular marker. The invention solves the technical problems that the traditional high-protein high-quality alfalfa new variety breeding is single plant selection according to the crude protein content of breeding offspring, and the method is time-consuming, labor-consuming and low in accuracy. The technical scheme of the invention is a molecular marker related to the content of crude protein of medicago sativa. The specific information is as follows: chromosome: chr3; the position is 25995949; sNP (Single Nucleotide Polymorphism) typing: C / A. The invention also designs a primer combination aiming at the molecular marker, and the primer combination can be used for directly and specifically distinguishing and detecting A or C basic groups of SNP mutation sites.

The invention provides zero-fat low-sugar yoghourt and a preparation method thereof, the zero-fat low-sugar yoghourt comprises the following raw materials: skimmed milk, a fermentation inoculant, a sweetening agent and a protein product, the fat content of the skimmed milk is less than or equal to 0.3 g / 100mL, the protein content of the skimmed milk is greater than or equal to 6.0 g / 100mL, and the lactose content of the skimmed milk is less than or equal to 4.0 g / 100mL; the fermentation inoculant is composed of pichia kudriavzevii, lactobacillus helveticus and streptococcus thermophilus, and the ratio of the three strains is 2: 1: 5. According to the zero-fat low-sugar yoghourt disclosed by the invention, through selection of specific strains, good stability can be realized under the condition that a thickening agent and a stabilizing agent are not added, the product can be endowed with better oxidation resistance, human body metabolism improving efficacy and immunity regulating efficacy, and a green, clean and healthy yoghourt product is really obtained.

The invention discloses a method for producing feed by using corn sugar residues, and belongs to the technical field of fermentation. The method comprises the following steps: pretreating, proportioning, adding an antibacterial agent, fermenting, drying and crushing, coating, cooling and the like, mixing corn sugar residues, corn germ meal and threonine mother liquor according to a specific ratio, adding zinc oxide and a chitosan antibacterial agent, and inoculating Saccharomyces cerevisiae ATCC 9763 for fermenting. And performing multi-stage drying and crushing on the fermentation product, spraying a coating emulsion prepared from a defatted soybean meal extract, curcumin, vitamin E, lecithin and sophorolipid, and performing air cooling to prepare the feed. According to the method, by-products are fully utilized, the crude protein content of the feed is 32-34.5%, the diameter of an inhibition zone is 18.1-20.2 mm, the moisture absorption rate is 2.7-3.4%, the palatability score is 8.0-8.6, and the fermentation yield is 92-94%. The innovation point lies in specific strain fermentation and functional coating, and is suitable for industrial production.

The invention provides a spleen peptide for promoting growth and tonifying spleen as well as a preparation method and application thereof, and belongs to the technical field of biochemistry. The invention provides a preparation method of spleen peptide capable of promoting growth and tonifying spleen. The preparation method comprises the following steps: degreasing spleen particles of healthy animals, fermenting after degreasing, performing subcritical water extraction, performing protease enzymolysis, fermenting an enzymolysis product, clarifying fermentation liquor, and then performing filtration, nanofiltration and spray drying treatment. According to the preparation method, the preparation process of the spleen peptide is reasonably set, so that the prepared spleen peptide is high in protein content, low in average molecular weight, easy to absorb and utilize, free of fat detection, capable of tonifying the spleen and promoting growth, capable of being dissolved into water to form transparent liquid, capable of being processed into tablets, powder and oral liquid, also capable of being used in common food, high in yield and suitable for popularization and application. The method is suitable for industrial popularization.

The invention discloses a preparation technology of chickpea plant-based milk, and relates to the technical field of food processing. Comprising the following steps of cooking, crushing, homogenizing, first-time enzymolysis, pH adjustment, second-time enzymolysis, enzyme deactivation, filtering, ingredient blending, high-speed shearing, homogenizing, sterilizing and canning. According to the chickpea plant-based milk with the low glycemic index prepared on the basis of the enzymolysis technology, the protein content reaches 1.86 + / -0.05 g / 100g, the protein utilization rate is increased by 38.4%, and the fat content is 0.85 + / -0.13 g / 100g; the content of dietary fibers is 1.51 + / -0.29 g / 100g; the eGI value is 57.27 + / -1.35; the average particle size of the chickpea plant milk is 124.49 microns; the absolute value of the Zeta potential is 55.67 + / -2.16 mV; 11 flavor substances contributing to sense organs are detected in the plant milk, the content of beany flavor substances such as hexanal is reduced, other flavor substances are enhanced, and the plant milk is endowed with mellow fragrance, light fruit fragrance and more distinct layering sense.

The invention discloses a beef cattle straw feed preparation method based on compound strain synergistic fermentation, and relates to the technical field of feed processing, and the beef cattle straw feed preparation method comprises the following steps: screening bacillus, lactic acid bacteria and the like to construct an initial compound strain, and screening a high-degradation-ability strain combination through a directed evolution system (30-45 DEG C, pH 4.0-7.0, and lignocellulose 5-15g / L); the straw lignocellulose structure is destroyed through microwave-assisted pretreatment (the power is 200-800 W, and the time is 1-5 min); high-efficiency fermentation is realized through double-stage fermentation (the pH is reduced at 30-35 DEG C for 12-24 hours in the first stage, and stubborn components are degraded at 35-40 DEG C for 36-60 hours in the second stage) and intelligent regulation and control. According to the method, the content of crude protein in the straw is increased to 15.2%, the content of crude fibers is reduced to 19.8%, the degradation rate of lignocellulose reaches 62%, the quality of the feed is remarkably improved, and a new path is provided for recycling of agricultural wastes.

The invention discloses bacillus subtilis, a method for preparing yellow corn silage feed under a strong alkali condition and a product thereof, and belongs to the technical field of microorganisms. The technical problem to be solved is that the survival rate of the existing bacillus subtilis is lower than 30% under the strong alkali condition of pH 9-11, and the existing bacillus subtilis cannot normally grow and propagate. The preparation of the yellow corn silage feed under a strong alkali condition needs additional pH adjustment, the process is complex and the cost is high; the crude protein content of the existing yellow corn silage feed is only 3-5%, and the requirements of livestock cannot be met. According to the technical scheme, the strong-alkali-resistant bacillus subtilis is provided, the preservation number is CGMCC No.36677, the survival rate of the strain is larger than or equal to 80% under the condition that the pH is 9-11, the straw degradation rate is larger than or equal to 25%, no waste water is generated when the strain is used for preparing the yellow silage feed, the cost is reduced compared with an existing technology, the protein content is increased by 50%-80%, palatability is good, and the method is suitable for large-scale straw resource utilization and feed protein production.

The invention provides a fermentation method for producing single-cell protein from corn straw. The fermentation method comprises the following steps: (1) inoculating a microbial seed solution into a crushed corn straw solid culture medium; (2) after the solid-state fermentation is completed, adding water, and reacting at 65-85 DEG C for 4-8 hours; and (3) cooling the liquid culture medium, and then inoculating a microbial seed solution for fermentation. By adopting the method, the yield of single-cell protein can be up to 142.68 g / L, and the yield of crude protein can be up to 44.8%. Compared with direct fermentation of the corn straw, the yield of single-cell protein and the content of crude protein can be remarkably increased, and the fermentation method for achieving efficient utilization of the corn straw is provided.