497 results about "Protein content" patented technology

The invention relates to the technical field of plant breeding, in particular to a molecular marker related to oil content and protein content of soybean and application of the molecular marker. The molecular marker is constructed on the basis of SNP sites; based on a genome version number Wm82. A2. V1, the SNP site is located at the 8605814th site of the No.8 chromosome of the soybean, and the polymorphism is A / G. The application comprises the following steps: (1) predicting or detecting the oil content or protein content of soybeans; (2) identifying or cultivating soybean varieties with high oil content or protein content; (3) soybean molecular marker assisted breeding; (4) soybean variety improvement related to oil content or protein content; and (5) soybean germplasm resource improvement. The molecular marker related to the soybean oil content and the protein content is obtained through research and screening, and the molecular marker can be applied to cultivation of soybean varieties with high oil content and high protein content and has important value in the field of soybean breeding.

The invention discloses a culture method for increasing the protein content of aspergillus niger mycelia by using corn steep liquor as a culture medium, and belongs to the technical field of biological fermentation. Aiming at insufficient resource utilization of corn steep liquor and substitute protein production requirements, a corn processing by-product corn steep liquor is creatively adopted to directly replace a traditional PDB culture medium, and the mycelium protein content is remarkably increased by optimizing a fermentation system. Results show that the corn steep liquor is used as a culture medium, aspergillus niger with the preservation number of CCTCC M 2013703 is inoculated, and aspergillus niger mycelium protein can be obtained through fermentation. The protein content of the mycelium obtained by the process disclosed by the invention reaches 50.62-53.56%, and the total amino acid content is increased by 64-76%. According to the method, high-value conversion of agricultural corn byproducts is realized, and an efficient and low-cost industrial solution is provided for alternative protein production.

The invention relates to the technical field of medicine analysis and detection, and particularly discloses a method for detecting the content of protein in a 3D-MLA adjuvant, which comprises the following steps: (1) preparing a test solution: firstly dissolving the 3D-MLA adjuvant by using a hydrochloric acid solution, performing 300-600W ultrasonic treatment for 2-5min, centrifuging 10000-13000g for 4-7min, and taking supernate; adding a compound enzyme into the supernate for enzymolysis; then adding concentrated hydrochloric acid, uniformly mixing, hydrolyzing for 20 + / -2 hours in a nitrogen atmosphere at 105-115 DEG C, blow-drying, and dissolving with a hydrochloric acid solution to obtain a test sample stock solution; adding a derivatization reagent into the test sample stock solution, and carrying out derivatization treatment, extraction and dilution to obtain a test sample solution; and (2) high performance liquid chromatography determination. According to the detection method provided by the invention, the residual quantity of the host cell protein in the 3D-MLA can be rapidly and accurately determined, and the quality control of the 3D-MLA is realized.

The invention relates to a wheat protein content prediction method and device and a medium, and relates to the technical field of protein content prediction.The wheat protein content prediction method comprises the following steps that near infrared spectrum data of wheat and the corresponding protein content are obtained, and global feature vectors and partition feature vectors of the near infrared spectrum data are extracted; adopting a multi-layer perceptron MLP as a base to construct a wheat protein prediction model; fusing the global feature vector and the partition feature vector to construct a wheat spectral feature vector, and inputting the wheat spectral feature vector and the protein content into a wheat protein prediction model for training to obtain a trained wheat protein prediction model; collecting near infrared spectrum data of wheat to be predicted; and inputting near infrared spectrum data of to-be-predicted wheat into the trained wheat protein prediction model to obtain a protein prediction value of the to-be-predicted wheat. According to the invention, rapid and lossless prediction of the wheat protein content is realized.

The invention discloses a cross-platform soybean seed protein content estimation method based on a spectral time sequence. The method comprises the following steps: step 1, acquiring high spectral data of a soybean canopy; 2, collecting a soybean multispectral image; step 3, acquiring soybean physiological parameters and grain protein content data; step 4, obtaining soybean photosynthetic parameter data; step 5, calculating a vegetation index for estimation; step 6, constructing a vegetation index time sequence; 7, screening an optimal time window; 8, extracting dynamic characteristics; step 9, establishing a model, estimating the soybean protein content, and performing precision verification; and step 10, carrying out mobility analysis on the model from a near-ground canopy to a satellite platform. According to the method, a theoretical basis and a practical path are provided for realizing remote sensing-based soybean GPC accurate monitoring, wide application of the method on a satellite remote sensing platform can be promoted in the future, and high-precision, high-time-efficiency and large-scale soybean GPC estimation is realized.

The invention discloses bacillus subtilis, a method for preparing yellow corn silage feed under a strong alkali condition and a product thereof, and belongs to the technical field of microorganisms. The technical problem to be solved is that the survival rate of the existing bacillus subtilis is lower than 30% under the strong alkali condition of pH 9-11, and the existing bacillus subtilis cannot normally grow and propagate. The preparation of the yellow corn silage feed under a strong alkali condition needs additional pH adjustment, the process is complex and the cost is high; the crude protein content of the existing yellow corn silage feed is only 3-5%, and the requirements of livestock cannot be met. According to the technical scheme, the strong-alkali-resistant bacillus subtilis is provided, the preservation number is CGMCC No.36677, the survival rate of the strain is larger than or equal to 80% under the condition that the pH is 9-11, the straw degradation rate is larger than or equal to 25%, no waste water is generated when the strain is used for preparing the yellow silage feed, the cost is reduced compared with an existing technology, the protein content is increased by 50%-80%, palatability is good, and the method is suitable for large-scale straw resource utilization and feed protein production.

The invention discloses an Indel marker related to a corn kernel protein content character and application of the Indel marker, and belongs to the technical field of plant molecular genetics and crop molecular design breeding. The Indel marker is located on a gdh2 gene of a chromosome 10 of a corn genome, and insertion or deletion of a nucleotide sequence as shown in SEQ ID NO.5 occurs in an intron region between a second exon and a third exon. By applying the molecular marker-assisted selection technology, high-protein new germplasms S3317 (the crude protein content is 17.0%) and JS231148 (the crude protein content is 15.6%) have been successfully cultivated, the increase amplitudes of the high-protein new germplasms and JS231148 are both more than 15% compared with those of the conventional control, and the lysine content reaches 0.41% and reaches the national high-quality protein corn standard.

The invention relates to the field of plant growth regulators, in particular to a conditioner group for improving the grain quality and the rice quality of direct seeding rice and a preparation and use method thereof.The preparation method of a conditioner in the initial heading stage comprises the following steps that 1, 6-benayl aminopurine is dissolved in a diluted hydrochloric acid solution in a assisted mode, and a 6-benayl aminopurine solution is obtained; step 2, adding choline chloride into the solution obtained in the step 1, stirring to obtain a mixed solution, transferring the mixed solution into a volumetric flask, and adjusting the pH value; 3, abscisic acid powder is dissolved with absolute ethyl alcohol, volume metering is carried out after dissolution to form a stock solution, and then PH adjustment is carried out. Two conditioners are included and sprayed in the initial heading stage and the full heading stage respectively, glumous flower degeneration can be reduced, the photosynthetic production capacity of the upper three leaves after flowering can be improved, assimilation substances are promoted to be transferred to grains, especially the filling state of vulnerable grains is improved, and the yield of the seedlings is increased. Finally, the grain number, the maturing rate, the grain weight and the yield are improved, meanwhile, the head rice rate of rice can be improved, and the protein content is reduced, so that the palatability of rice is improved.

The invention provides a method for improving forage grass production performance and silage quality and application of the method, and belongs to the technical field of silage production. The method comprises the following steps that after silage corn is sown, lacroton beans are sown between two rows of corn immediately; first topdressing is carried out 18-22 days after sowing; carrying out secondary topdressing 60-68 days after sowing; harvesting is conducted on the 105th-110th day after sowing is conducted; and carrying out silage on the harvested silage corn and the harvested lacroton beans. Through mixed sowing of the corn and the lab beans, the dry matter yield and the crude protein content of the mixed forage grass are improved, supply of protein raw materials in concentrated feed is reduced, and the feed cost is reduced. The abundance of leuconostoc can be remarkably reduced during the ensiling period, and the silage obtained after ensiling is excellent in sensory evaluation. The content of nutritional ingredients in the silage is changed, protein and carbohydrate components are changed, and the nutritional value of the silage is improved.

The invention relates to the technical field of food quality detection, and discloses a meat product comprehensive scoring method based on principal component analysis, and the method comprises the steps: firstly obtaining physicochemical index (such as protein and fat content) and sensory attribute (such as color saturation and elasticity index) data of multiple batches of samples, and carrying out the structural arrangement of the two types of data according to batches and index dimensions; respectively carrying out Z-score standardization, range normalization and other processing, and extracting a physicochemical index feature vector and a sensory attribute feature vector; constructing a dynamic weight strategy through a variance contribution rate and a feature root ratio, and performing weighted fusion on the two types of feature vectors to obtain a fused feature vector; and finally, inputting the data into a scoring model, and after centesimal system conversion and distribution calibration, dividing quality grades in combination with an industry standard threshold to generate a comprehensive scoring result containing alphanumeric identifiers. The method realizes multi-dimensional data fusion and dynamic empowerment, improves the scoring accuracy, and is suitable for meat product quality evaluation.

The invention discloses a biological energy feed fermentation yeast based on enzyme-mineral synergistic activation and a preparation method thereof. According to the scheme, dual functions of mineral activation and microbial conversion are focused, clinoptilolite, sodium bentonite and kaolin are taken as mineral carriers, lignite humic acid is taken as a nutrient synergist, brown sugar, rice bran and bran organic matrixes are matched, and mineral lattice structures are broken through mechanical crushing and biological enzymolysis pretreatment, so that the mineral active substances are obtained. And through a double-stage fermentation process of'aerobic fermentation to activate flora and anaerobic aging to enrich metabolites' of microorganisms, mineral ions, humic acid active ingredients and probiotic metabolites are released to the maximum extent, and finally, the high-quality biological energy feed fermentation yeast with nutrition supply, digestion promotion and immune regulation functions is formed. According to the bioenergy feed prepared by conversion of the fermentation yeast, the feed crude protein improvement rate is greater than or equal to 20%, the crude fiber degradation rate is greater than or equal to 40%, the feed conversion rate is improved by 10-15%, the intestinal disease incidence rate is reduced by 60-70%, and the bioenergy feed is suitable for various livestock and poultry and aquaculture scenes.

The invention discloses an enzyme-linked immuno sorbent assay (ELISA) technology-based detection optimization method for A2 protein content in milk, and relates to the technical field of protein content detection.The method comprises the following steps: collecting a milk matrix interference detection data set, and establishing a milk matrix interference corrector; generating an ELISA dynamic detection module; preparing a milk sample to be detected, and controlling the ELISA dynamic detection module to perform protein optimization detection on the milk sample to be detected to obtain milk absorbance detection data; and establishing an absorbance-concentration standard curve, and determining the detection result of the A2 protein content of the milk. The technical problems that in the prior art, when the A2 protein in milk is detected through ELISA, the detection accuracy and reliability are poor due to the fact that the A2 protein in the milk is easily interfered by components and the adaptability to different types of milk is insufficient are solved, the A2 protein content in the milk can be detected more accurately, the anti-interference capacity is higher, and the detection accuracy and reliability are improved. The technical effect of improving the accuracy and the reliability of the ELISA technology for detecting the A2 protein of the milk is achieved.

The invention discloses application of GmABHD6 protein in controlling soybean oil content or soybean protein content, and belongs to the technical field of soybean quality. The invention aims to improve the quality of soybeans and increase the oil content and protein content of the soybeans. The invention discloses application of GmABHD6 protein in controlling soybean oil content or soybean protein content or increasing soybean yield. The amino acid sequence of the GmABHD6 protein is shown as SEQ ID NO.2. The invention further discloses application of the GmABHD6 protein in controlling soybean oil content or soybean protein content or increasing soybean yield. GmABHD6 negatively regulates and controls the oil content of soybean seeds, and a theoretical basis is provided for cultivation of high-quality soybeans.

The invention discloses a tenuilia microspora NB1-3, a leavening agent and application of the tenuilia microspora NB1-3 in preparation of caragana microspora and moringa oleifera fermented protein feed. The strain is classified and named as nicotiana microspora, and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 20251872. The core of the method is that crude fibers and antinutritional factors (tannin and phytic acid) in moringa oleifera and caragana microspora are efficiently degraded by utilizing a strong lignocellulose degrading enzyme system of the tenuilia microspora NB1-3, and meanwhile, the crude protein content of the feed is increased. The prepared fermented protein feed is high in crude protein content and low in crude fiber content and meets the protein feed standard, efficient and high-value utilization of woody plant resources such as caragana microphylla and moringa oleifera is achieved, and a new high-quality and economical protein feed source is provided for animal husbandry.

The application discloses a transcription factor for regulating protein content of rice grains GPC6 and application, the amino acid sequence of the transcription factor is shown as SEQ ID NO. 1, the transcription factor comprises a genomic region and a promoter, the nucleotide sequence of the genomic region is shown as SEQ ID NO. 2, the nucleotide sequence of the promoter is shown as SEQ ID NO. 3, the coding sequence (CDS) is shown as SEQ ID NO. 4, and all alleles thereof are contained. The application identifies a repeatedly detectable locus on chromosome 6 by performing whole genome association analysis on gluten content and total protein content GPC6 . Gene cloning is carried out on the locus, so that the genetic resources for improving rice quality are enriched, and help is provided for improving rice quality, in particular, protein content.

The present invention relates to a Single Nucleotide Polymorphism (SNP) marker for determining the degree of protein content of rice seeds and a method for determining the degree of protein content of rice seeds using said marker. Specifically, the present invention relates to an SNP marker for determining protein content and a method for determining protein content using said marker, which constructs a machine learning-based rice protein content prediction model by utilizing SNP markers associated with high and low protein content, thereby enabling the prediction of rice seeds with high protein content in the early stages of growth.

The objective of the present invention is to provide a method for manufacturing powdered cultured meat that can increase cell differentiation efficiency and protein content with economic feasibility, and to provide powdered cultured meat with high physicochemical stability and transportability, as well as a food composition containing the same. The powdered cultured meat has a flavor similar to real meat and, as a high-protein food, has excellent utility in various food compositions or processed foods.

The invention discloses a method for reducing the purine content of phaseolus calcaratus powder, and belongs to the technical field of edible bean processing. The method comprises the processes of acid-assisted hydrothermal treatment and isoelectric precipitation. The acid-assisted hydrothermal process comprises the following steps: mixing 0.1-1 mol / L of hydrochloric acid, 0.5-1 mol / L of phosphoric acid or 0.8-1 mol / L of citric acid with the phaseolus calcaratus powder, and stirring and heating at 60-70 DEG C; and the isoelectric precipitation is to precipitate and recover the phaseolus calcaratus protein under the condition that the pH value of the mixed system is 3-5. And freeze-drying the precipitate, and grinding to obtain the low-purine phaseolus calcaratus powder. The processing conditions can effectively reduce the purine content in the phaseolus calcaratus, so that the processing loss can be effectively controlled, and the higher protein content can be kept; the problem that the purine content in phaseolus calcaratus is high at present can be solved, and a new method is provided for research and development of low-purine phaseolus calcaratus products.

The invention provides high-whiteness and low-isoflavone-content soybean protein isolate for infant food, and belongs to the technical field of infant complementary food. Comprising the following components in percentage by mass: 92.5%-6% of soybean protein isolate, 6.0% of maltodextrin, 0.8% of sodium carboxymethyl cellulose, 0.1% of sucralose and 0.6% of ethyl vanillin, the risk of residual chemical bleaching agents (hydrogen peroxide and sodium hypochlorite) is avoided, the content of isoflavone is reduced to be less than or equal to 10mg / kg, the hidden danger of endocrine interference is reduced, the damage of high temperature and strong acid / alkali to a protein structure is reduced, and the quality of the protein is improved. The energy consumption is reduced through low-temperature sterilization and vacuum freezing dehydration, the whiteness is greater than or equal to 85%, the sensory requirements are met, the protein content is greater than or equal to 90%, the purity is high, and the protein is suitable for being used as a high-quality protein source of infant food; the green solvent and physical / biological decoloration reduce chemical pollution, and the process is more environment-friendly.

The invention relates to the technical field of information, and discloses a method for predicting the optimal ratio of arginine to lysine in daily ration of lactating sows, and the method comprises the following steps: 1, obtaining historical production data and feed formula data; 2, performing data preprocessing on the historical production data and the feed formula data to obtain historical feature vectors and formula sample data; 3, selecting a preset model with the best performance as a prediction model according to the performance indexes; 4, inputting the to-be-detected feature vector and the crude protein content into the prediction model, and outputting a proportion prediction value of arginine and lysine by the prediction model; 5, performing a field dose response test and acquiring field data; and step 6, inputting the field data into the prediction model, detecting whether data drift occurs in the field data, performing incremental learning when no significant drift occurs in the field data, and performing full-amount learning when significant drift occurs in the field data. The invention further discloses a device for predicting the optimal arginine and lysine ratio of the daily ration of the lactating sows.

The invention belongs to the technical field of biology, and particularly relates to Meyerozyma caribba XX2120, and the preservation number of the Meyerozyma caribba XX2120 is GDMCC No: 67777. The invention further relates to a preparation method of the Meyerozyma caribba XX2120. The strain is obtained by taking saccharomycetes X2120 as a starting strain, carrying out ARTP mutagenesis treatment and screening, has relatively strong acid resistance, relatively high salt stress resistance and certain alcohol resistance and aldehyde resistance, can effectively utilize monosaccharide, disaccharide and trisaccharide, has a wide utilization range on carbohydrate and has a relatively good utilization rate on inorganic nitrogen. Moreover, the strain can be used for producing mycoprotein through liquid fermentation by taking Luzhou-flavor distillers' grains as a raw material, and also can be used for performing solid fermentation on the Luzhou-flavor distillers' grains, so that the true protein content of the distillers' grains is increased, the nutritional value of the distillers' grains is improved, and the recycling of distillers' grain resources is effectively promoted.

The application discloses application of TCTP protein in preparation of noninvasive premature birth diagnosis products and relates to the technical field of biological medicine. The application can estimate the premature birth risk by detecting the TCTP protein content or the TCTP gene expression quantity in peripheral blood of a pregnant woman, the method only needs 0.5ml peripheral blood of the mother, is safe for the mother and infant, and has the advantages of simplicity, high sensitivity, low cost and simple operation. In 126 sample examples in the embodiment of the application, the premature birth detection rate of the method is 91%, and in the control group, the false positive rate is 8.6% (5 examples / 58 examples), so the method has high accuracy.

The invention discloses a method and a device for stimulating growth of oat seeds by steam jet negative corona discharge, after steam passes through an electric field, the oat seeds are stimulated by the steam jet negative corona discharge, the discharge intensity is controlled by selecting different discharge time, the oat seeds are treated, and the treated seeds are planted. The germination, growth and harvesting processes of the oat seeds are studied, and changes of components, structures and functional characteristics of mature oat powder are discussed; results show that the low-intensity (5 min) treatment significantly improves the crude protein content of the oats, reduces the total starch content, promotes the photosynthesis and plant growth of oat plants, increases the number of grains, increases the ordering degree and stability of the oat powder, and is beneficial to forming a colloid system which is rich in water and stable in structure. The invention proves that an oat product with high protein and low starch content can be obtained through proper steam jet negative corona discharge treatment, the microstructure is more ordered, and the gelatinization performance of the oat can be obviously improved.

The invention discloses candida utilis engineering bacteria and a method for producing single-cell protein by fermenting corn thin pulp, and belongs to the technical field of biology. According to the candida utilis engineering bacteria disclosed by the invention, the candida utilis is subjected to gene modification, a chitin synthase gene in the candida utilis is knocked out, and an exogenous keratinase gene is integrated, so that the candida utilis engineering bacteria can utilize corn thin pulp as a substrate for fermentation. In the fermentation process, carbon and nitrogen sources in the thin corn pulp can be effectively utilized for growth, macromolecular protein in the thin corn pulp can be hydrolyzed and converted into single-cell protein, the content of the single-cell protein in final fermentation liquor is increased, the protein content of the final fermentation liquor can reach 60% or above, and the proportion of the single-cell protein can reach 30% or above; the corn thin pulp can be efficiently utilized, and meanwhile, single-cell protein in the fermentation liquor can be used for adding animal feed so as to meet the requirement for animal protein feed in the market.

The invention provides a preparation method of silphium perfoliatum beef cattle breeding feed, and relates to the field of beef cattle feed. The silphium perfoliatum beef cattle breeding feed mainly consists of the following raw materials in parts by weight: 40-60 parts of silphium perfoliatum dry powder; 20 to 40 parts of crop straw powder; 5 to 15 parts of an energy supplement; 1-5 parts of a mineral premix; 0.1 to 1 part of a vitamin premix; the protein content of the silphium perfoliatum dry powder is not lower than 24%, and the crude fiber content of the silphium perfoliatum dry powder is not higher than 18%. The feed is prepared by taking silphium perfoliatum planted without chemical fertilizers and pesticides as a core, performing pretreatment of kneading, drying and crushing, matching straw fine powder in proportion, adding energy supplements, mineral substances and the like for compounding, stirring and granulating. Application shows that the feed is balanced in nutrition, can improve the daily average weight gain of beef cattle, reduce the morbidity, optimize the beef quality and reduce the breeding cost, and is suitable for large-scale beef cattle breeding.

According to the method for making the walnut meal noodles, the protein content of the noodles is increased by adding the walnut meal, meanwhile, the problem of processing performance degradation is solved by utilizing the synergistic effect of curdlan, edible salt and water, the nutritional ingredients in the walnut meal are reserved through a low-temperature drying technology, and balance of nutrition and quality is achieved. According to the method, the nutritional quality of the noodles can be remarkably improved, the protein content of the prepared walnut meal noodles reaches 13.34%, the high-protein dietary standard (larger than or equal to 13%) is met, the cooking loss rate is smaller than or equal to 7.5%, the elasticity is larger than or equal to 0.95 mm, the cost can be reduced, walnut meal waste recycling is achieved, the technology compatibility is high, and the practical popularization value is achieved.

The application belongs to the technical field of cottonseed meal processing and animal feed, and particularly relates to application of enzymatic cottonseed meal in preparation of low-protein daily ration, promotion of animal intestinal development, improvement of animal production performance and improvement of carcass quality, wherein the small peptide content, free amino acid content and antioxidant activity of the enzymatic cottonseed meal are all significantly improved compared with cottonseed meal, the change of protein secondary structure and the significant reduction of relative molecular weight are more conducive to promotion of animal absorption of small molecules, are beneficial to animal intestinal health development, and can effectively improve animal growth performance. The feed prepared by replacing part of the conventional feed with the enzymatic cottonseed meal can still achieve the effects of promoting animal intestinal development and improving animal growth performance under the condition that the crude protein content is reduced by 1% to 2% compared with the basic daily ration or the conventional feed, and the feeding cost is significantly reduced compared with the use of the conventional feed.