109 results about "Single-cell protein" patented technology

This disclosure describes methods to separate solids from liquids in a production facility. A process separates components in the process stream by applying non-condensable media to create density differences and then using a mechanical device to separate the solids from the liquids based on the density difference. The process produces the liquids and solids, which may be further processed to create valuable animal feed products.

The invention discloses kluyveromyces marxianus and application of the kluyveromyces marxianus in production of single-cell protein by taking straw as a raw material. The Kluyveromyces marxianus is a ZH-2 bacterial strain which is CGMCC (China General Microbiological Culture Collection Center) No.32295 The strain is obtained from a wild strain in a laboratory adaptive evolution mode, wheat straw and an inorganic nitrogen source can be used as raw materials for efficiently producing single-cell protein, compared with a parent strain, the utilization speed and the utilization rate of biomass, glucose and xylose are remarkably increased, the yield of ZH-2 SCP can reach 0.047 g.g <-1 >, and the yield of ZH-2 SCP can reach 0.045 g.g <-1 >. And the essential amino acid mode basically accords with adult and infant amino acid modes, and can be used as high-quality protein nutrition supply. The Kluyveromyces marxianus ZH-2 strain capable of obtaining single-cell protein by fermenting wheat straw as a raw material is provided from the perspective of utilizing cheap waste resources, so that the production cost of SCP is greatly reduced, and the problem of protein resource shortage is effectively solved.

The invention discloses candida utilis engineering bacteria and a method for producing single-cell protein by fermenting corn thin pulp, and belongs to the technical field of biology. According to the candida utilis engineering bacteria disclosed by the invention, the candida utilis is subjected to gene modification, a chitin synthase gene in the candida utilis is knocked out, and an exogenous keratinase gene is integrated, so that the candida utilis engineering bacteria can utilize corn thin pulp as a substrate for fermentation. In the fermentation process, carbon and nitrogen sources in the thin corn pulp can be effectively utilized for growth, macromolecular protein in the thin corn pulp can be hydrolyzed and converted into single-cell protein, the content of the single-cell protein in final fermentation liquor is increased, the protein content of the final fermentation liquor can reach 60% or above, and the proportion of the single-cell protein can reach 30% or above; the corn thin pulp can be efficiently utilized, and meanwhile, single-cell protein in the fermentation liquor can be used for adding animal feed so as to meet the requirement for animal protein feed in the market.

The invention relates to the technical field of microbial fermentation, and provides a method for producing single-cell protein, which comprises the following steps: inoculating a crop straw matrix with a first microbial seed for first fermentation; the first microbial seed comprises trametes versicolor; sterilizing a fermentation product obtained by the first fermentation; inoculating second microbial seeds into the sterilized fermentation product for second fermentation; the second microbial seed comprises yarrowia lipolytica / candida utilis. According to the scheme, the combination of the selected first microbial seed solution and the second microbial seed solution has a remarkable synergistic effect, so that the crude protein content, the lignin removal rate, the cellulose retention rate and the yeast biomass are improved, and the hemicellulose loss rate is reduced.

The present application relates to the field of feed raw material crushing device, specifically to a single-cell protein feed raw material processing crushing device, including the crushing tank, the top of the crushing tank is fixedly installed with the driving motor, the lower part of the inner wall of the crushing tank is fixedly connected with the filter plate, the top of the filter plate is rotatably connected with the transmission rod, and the top of the transmission rod is fixedly connected with the rotating end of the driving motor, further including: fiber collection mechanism. The single-cell protein feed raw material processing crushing device adjusts the cooperation of the adjusting ring and the expansion plate, periodically drives the movable blade disc to approach the fixed blade disc, in this process, on the one hand, the fiber wound on the fiber collection rod is pushed into the cutting area, on the other hand, the sharp blades arranged in a staggered manner are used for accurately and efficiently secondary crushing of the fiber, which makes the waste originally affecting production be converted into fine powder and re-mixed into the feed product, thus eliminating the hidden trouble and improving the comprehensive utilization rate of the raw material.

A Pichia pastoris strain and its application in the fermentation production of single-cell protein belong to the field of microbial fermentation technology. The Pichia pastoris strain... Komagataella phaffii GG7 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 39081, on December 2, 2025. This Pichia pastoris strain GG7 was isolated from soil in a methanol production plant area. It maintains good growth even in high concentrations of methanol (up to 8% (w / v)) and mixed carbon sources such as methanol, acetic acid, and ethanol, achieving a single-cell protein content of approximately 62 wt%. This strain is suitable for aerobic fermentation using methanol, ethanol, and / or acetic acid as carbon sources, exhibiting strong carbon source tolerance and high protein yield, making it suitable for industrial production of single-cell protein.

The present invention relates to an improved method for culturing microorganisms capable of utilizing ethanol as a starting material using continuous culture. The present invention relates to an improved process for the production of biomass, in particular single cell proteins, using continuous culture, wherein the yeast single cell protein product comprises yeast cells of the Saccharomyces cerevisiae; and an animal feed comprising such biomass.

The present application belongs to the field of environmental biotechnology and resource recycling technology, and provides a high-ammonia-nitrogen-tolerant yeast strain and its application in biogas slurry resource treatment. The high-ammonia-nitrogen-tolerant yeast strain is named Torulaspora delbrueckii L12345, which was preserved in the China General Microbiological Culture Collection Center (CGMCC) on January 13, 2026, and the address is No. 1, Yitian West Road, Chaoyang District, Beijing, China Institute of Microbiology, Chinese Academy of Sciences, and the preservation number is CGMCC No. 39123. The present application realizes efficient treatment of biogas slurry and simultaneous production of single-cell protein, reduces the treatment cost, improves the economic benefit, and provides a new technology for clean and high-value utilization of biogas slurry.

A control method and apparatus are provided for the safe operation of aerobic gas fermentation by a C1-fixing microorganism to produce various products such as single cell protein or cultured protein. The aerobic fermentation comprises feeding specific gases (one of which is oxygen) into a bioreactor where they're fermented to products such as single cell protein or cultured protein. Specifically, the control apparatus and method involve measuring the O2 concentration in the headspace above a liquid fermentation broth (in a bioreactor) comprising a C1-fixing microorganism and determining if the concentration is at or below a predetermined set point. Using various sensors, flow controllers and transmitters plus a control system, the flow rate of the O2 comprising gas stream into the bioreactor is adjusted or shut off to maintain the O2 concentration in the headspace below the set point.

The present application relates to the technical field of animal feed, more particularly, to a methanol-based single-cell protein feed composition and a preparation method thereof.The feed composition comprises the following raw material components by weight: 30-70 parts of methanol-based single-cell protein powder, 10-40 parts of plant protein source, 10-30 parts of energy source, 1-10 parts of functional additive, and 5-20 parts of carrier.The present application uses the methanol-based single-cell protein, a non-grain protein source with high protein and good amino acid composition, to significantly improve the palatability, digestibility and nutritional value of the feed by compounding the methanol-based single-cell protein with conventional feed components through a scientific formula and a physical wall-breaking and mixing process, and to partially or completely replace traditional protein sources such as soybean meal, thereby reducing the cost of feed, alleviating the problem of food competition between humans and animals, and providing a sustainable new protein feed solution for the animal husbandry.

Food products are provided in which microbial protein sources, such as single cell protein or protein hydrolysis products, are incorporated, and which contain proteins that are recombinantly expressed in chemoautotrophic microorganisms. Meat, dairy, and egg substitute products are provided that include microbial protein and proteins that are recombinantly produced in chemoautotrophic microorganisms in place of animal protein.

The present disclosure is directed to methods for measuring proteomes of single cells. In one aspect, methods comprise disposing cells on a surface, synthesizing hydrogel chambers around each of the cells, lysing the cells to release intracellular proteins for adsorption onto the surface bounded by the hydrogel chambers, depolymerizing the hydrogel chambers, and quantifying the adsorbed proteins with detection antibodies to determine the single cell proteomes.

The application discloses a single-cell immunoblotting method based on quenchable aptamer probes and relates to the technical field of single-cell protein detection. The method comprises the following steps: S1, a single-cell suspension sample is settled in a microwell array on a composite hydrogel chip; S2, the composite hydrogel chip is placed in an electrophoresis tank, then a lysis solution is slowly poured into the tank, and incubation lysis is carried out; S3, after cell lysis, an electric field is applied to both ends of the chip, and proteins enter a gel coating layer on the surface of the chip under the action of the electric field and start electrophoretic separation; S4, after gel electrophoresis is completed, the chip is placed under an ultraviolet lamp for exposure, and the gel is taken out after exposure is completed; S5, the gel is placed in an aptamer probe solution which is targeted to target proteins, and is soaked and dyed, and the fluorescence signal intensity of the target protein molecules is measured under a laser confocal fluorescence microscope. The application can improve the detection sensitivity of target proteins and has fast imaging speed.

Provided is a method for nucleic acid reduction in a bacterium to less than about 5 wt % of a total dry weight of the bacterium, the method comprising culturing the cells of the at least one bacterium in a fermentation medium to obtain a fermentation medium comprising a biomass; heating the biomass to a temperature of at least 70° C. for at least 10 minutes; and washing the biomass. Also provided are food components, food ingredients and foods or beverages comprising single cell proteins produced from such bacteria.

The present application provides a base model processing method and device for single-cell proteome detection data, which comprises: obtaining single-cell proteome detection data, uniformly processing protein identification information, and standardizing protein abundance data according to detection technology types to obtain uniformly distributed protein expression data; mapping relevant information into identification and abundance embedding vectors respectively and fusing them, adding a special marker vector to construct cell sequence data; inputting the cell sequence data into a base model for pre-training, optimizing parameters through a mask reconstruction and global prediction task to obtain a pre-training model; and fine-tuning the model for a target analysis task, analyzing and processing to-be-processed data to obtain downstream results. The present application can integrate single-cell proteome data of different detection technologies and batches, effectively remove batch effects, extract stable cell low-dimensional representations, support multi-downstream task fine-tuning, realize undetected protein inference and cell trajectory analysis, and improve data utilization efficiency and task adaptability.

The invention belongs to the technical field of synthetic biology and fermentation engineering, and particularly relates to a coupling fermentation method for synthesizing single-cell protein from methanol and carbon dioxide. The coupling fermentation method comprises the following steps: mixing and stirring a fermentation medium and methanol according to a weight part ratio of (97-99): (1-3) to form a fermentation substrate mixed solution; mixing the pre-cultured saccharomycetes liquid, the pre-cultured photosynthetic bacteria liquid and the fermentation substrate mixed liquid according to a weight part ratio of (2-4): (3-6): (90-95), introducing a mixed gas source, and carrying out illumination fermentation to obtain fermentation liquid; and sequentially carrying out centrifugal separation and vacuum drying on the fermentation liquor to obtain single-cell protein. According to the method, the pre-cultured yeast liquid, the pre-cultured photosynthetic bacterium liquid and the fermentation substrate mixed liquid are mixed and then are subjected to aerated fermentation, so that the fermentation capacity of microorganisms at a relatively high fermentation temperature is improved, and the yield of single-cell protein is increased.

This invention provides a method for producing single-cell protein using a mixed bacterial culture, comprising the following steps: performing primary seed culture on methanogenic bacteria, *Tricholoma materata*, and *Glucosinolate methyl-eating bacteria* to obtain primary seed solutions; mixing the primary seed culture solutions evenly to obtain a mixed bacterial solution; inoculating the obtained mixed bacterial solution into a secondary seed culture medium for cultivation to obtain a secondary seed solution; inoculating the obtained secondary seed solution into a fermentation medium for scale-up cultivation to obtain a fermentation broth; separating the solid and liquid components of the obtained fermentation broth, harvesting the bacterial cells, and obtaining single-cell protein. This method for producing single-cell protein using a mixed bacterial culture improves the utilization rate of the carbon source methane and the yield of single-cell protein.

The invention discloses an integrated feeding control method for acetic acid group single-cell protein fermentation and application of the integrated feeding control method, and belongs to the technical field of microbial fermentation. By monitoring the change of the pH value of the fermentation liquid in real time, the composite material supplementing system containing the carbon and nitrogen sources is automatically triggered, pH signals are converted into the supplementing amount of the carbon sources and the nitrogen sources, the stable carbon-nitrogen ratio of supplemented materials in the fermentation process is maintained, and meanwhile the pH value is maintained in an appropriate range. The thallus yield and the protein content of the single-cell protein are obviously improved; the problems of poor thallus growth, low protein content and large substrate residual quantity caused by mutual independence of a carbon source, a nitrogen source and pH adjustment, tedious control of a fermentation process and imbalance of a carbon-nitrogen ratio in an existing material supplementing mode are solved.

The present invention relates to a method for pasteurizing biomass, such as fungal biomass, in which the biomass is pasteurized using specific time and temperature parameters to obtain pasteurized biomass. The pasteurized biomass is suitable for use as a source of single cell proteins in food products. The invention also relates to pasteurized products, and to food products comprising same.

The invention relates to a method for producing single-cell protein by fermenting kitchen waste, which comprises the following steps: 1) performing acid dissolution and alkali dissolution treatment on the kitchen waste, and then filtering to obtain a homogeneous suspension of the kitchen waste; 2) sterilizing the kitchen waste homogeneous suspension at high temperature, cooling and inoculating strains; and (3) fermenting the homogeneous suspension of the kitchen waste inoculated with the strain, and centrifuging and drying the fermentation liquor when a certain thallus concentration is reached, so as to obtain a single-cell protein product. According to the method, microorganisms are utilized to grow and ferment nutritional ingredients such as grease and carbohydrates in the kitchen waste as substrates, and single-cell protein rich in crude protein is produced. According to the method, the utilization rate of the kitchen waste is remarkably increased, high-quality single-cell protein rich in protein can be produced, the protein can be used as an animal feed product, and a circular, economical and sustainable method is provided for solving the problem of the kitchen waste.

The application discloses a Candida utilis strain for efficiently converting ethanol / acetic acid and application thereof, and belongs to the technical field of single-cell protein production. In view of the technical problems of limited biomass accumulation, low carbon source conversion efficiency and low protein yield of a wild-type Candida utilis strain when the mixed carbon source of ethanol and acetic acid is used, the application provides the Candida utilis TU546 strain with the preservation number of CGMCC NO. 39030, and the strain is cultured in a fermentation medium containing the mixed carbon source of ethanol and acetic acid, so that the fermentation strain body with high protein content is obtained, and efficient conversion from the mixed carbon source of ethanol and acetic acid to single-cell protein is realized. The strain and the method thereof are mainly used for producing single-cell protein products, and can be used as feed or food additives, and have important application value in the microbial fermentation industry.

The invention provides a system and a method for co-producing single-cell protein through electrodialysis treatment of organic wastewater and hydrogen oxidizing bacteria fermentation, the system and the method are divided into an anode chamber, a middle chamber and a cathode chamber through a cation exchange membrane and an anion exchange membrane, and the anode chamber and the cathode chamber are filled with organic wastewater; and the middle chamber is filled with a hydrogen oxidizing bacteria fermentation culture medium. Under the driving of an electric field, ammonium ions and acetate ions in the wastewater are selectively migrated to the middle chamber through the anion and cation exchange membrane respectively, and are efficiently utilized by hydrogen oxidizing bacteria as a nitrogen source and a carbon source to produce single-cell protein. According to the system, collaborative integration of an electrodialysis technology and a microbial fermentation process is successfully realized, nutrient substances in the organic wastewater are recovered and converted into single-cell protein with high additional value while the organic wastewater is purified, the treatment burden is effectively converted into protein resources, and the system has environmental benefits, economical efficiency and sustainability.

The application provides a vinegar residue-based single-cell protein feed and a preparation method and application thereof, and belongs to the technical field of feed preparation.The application uses vinegar residue as raw material, adopts solid-state fermentation of Aspergillus, Candida utilis and Saccharomyces cerevisiae in combination with composite enzymes, utilizes the synergistic effect among the strains and the enzymolysis effect of the exogenous enzymes, efficiently degrades the fibrous substances in the vinegar residue, and converts the fibrous substances into microbial proteins, so that the crude protein content of the prepared vinegar residue-based single-cell protein feed is increased from 5% to 8% of the raw material to 20% to 25%, and the total fibrous substance degradation rate is greater than or equal to 60%. The vinegar residue-based single-cell protein feed produced by the application has good feeding value, can effectively replace part of the protein raw materials such as soybean meal, improves the production performance of beef cattle, reduces the breeding cost, realizes high-value utilization of vinegar residue resources, and has good popularization and application prospect.

The invention discloses pichia pastoris and application thereof in fermentation production of single-cell protein, and belongs to the technical field of microbial fermentation. The pichia pastoris is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number of the pichia pastoris is CGMCC No.39081, and the preservation time of the pichia pastoris is December 2, 2025. The pichia pastoris GG7 is separated from soil of a methanol production plant and can keep good growth in high-concentration methanol (the highest concentration is 8% (w / v)) and a mixed carbon source of methanol, acetic acid, ethanol and the like, and the content of single-cell protein can reach about 62 wt%. The strain is suitable for aerobic fermentation with methanol, ethanol and / or acetic acid as a carbon source, has the advantages of high carbon source tolerance, high protein yield and the like, and is suitable for industrial production of single-cell protein.

The present invention relates to a method for culturing a microorganism, preferably a yeast of the Saccharomyces cerevisiae. The present invention relates to a method for increasing the protein content, in particular single-cell proteins, in biomass production, wherein a yeast single-cell protein product comprises yeast cells of the Saccharomyces cerevisiae; and an animal feed comprising such biomass.

The application is suitable for the technical field of single-cell protein production, and provides a fermentation preparation device for single-cell protein, which comprises a support frame with a first platform, a second platform, a third platform and a fourth platform arranged in sequence from bottom to top; a first-stage seed tank, a second-stage expansion tank, a main fermentation tank and a centrifuge installed on the fourth platform, the third platform, the second platform and the first platform from top to bottom respectively, and used for strain activation, expansion culture for improving biomass of bacterial cells, biological fermentation and protein separation; the first-stage seed tank, the second-stage expansion tank and the main fermentation tank are all provided with a discharge pipe at the bottom for communication between adjacent tanks, and the discharge pipe is provided with an electromagnetic valve. The fermentation preparation device for single-cell protein provided by the application has a vertical layout, small floor area, and a single motor can selectively drive any or multiple stirring shafts, so that the energy consumption and maintenance cost of the equipment are significantly reduced.

The present invention is generally concerned with the field of microbial fermentation of dairy or dairy alterative industry waste material and microbial protein production such as single cell protein (SCP). In particular the present invention is directed to microbial fermentation of dairy derived or dairy alternative derived whey for production of single cell proteins (SCP) or microbial biomass. More in particular, the present invention relates to a method of producing a single cell protein or biomass, comprising propagating a microbial culture in a large scale setting under suitable conditions and growth medium comprising dairy-derived whey to produce a single cell protein, where the microbial culture comprises microalgae, yeast and bacterial species (Figure 1). The present invention also relates to method of identifying a strain of microorganism, or mixture of strains of microorganisms, capable of efficiently using dairy-derived whey waste as a food source to produce biomass as a source of single cell protein. The present invention also relates to a propagated microbial culture or a composition and use of the culture in microbial fermentation. The present invention further provides a product or supplement comprising the composition of the present invention.

The invention discloses a method for enhancing the synthetic ability of yeast total protein and dipeptide tripeptide. Relates to the technical field of synthetic biology and metabolic engineering. According to the invention, the saccharomyces cerevisiae engineering strain with high protein synthesis capability and high endogenous dipeptide and tripeptide accumulation level is successfully constructed. The metabonomics analysis reveals the unique peptide fragment accumulation spectrum, especially the generation of a large amount of lysine-rich tripeptide at the C terminal, and proposes a potential mechanism of metabolic pathway'bottleneck 'caused by peptidase activity down-regulation in combination with proteome data. The recombinant strain has important application potential in the fields of single-cell protein, functional peptide products and the like.

The present invention relates to a novel fermentation process using a particular strain of Kluyveromyces marxianus, referred to as KMPROTEO 1, to obtain a single-cell protein useful in the food industry.In the present description, the invention discloses a process for fermenting dextrose and other consumables to obtain a single-cell biomass. The disclosed process enables a high-protein product to be obtained that is in the range of 50 to 60% for use in the food industry. The disclosed process uses dextrose as a propagation medium for use by Kluyveromyces marxianus to obtain a single-cell biomass and, subsequently, a single-cell protein.

A bacterial strain of the genus Xanthobacter and continuous culture processes for the production of protein or biomass using bacteria of the genus Xanthobacter, said process including supply of gases and minerals to the cells. The present disclosure also relates to the products of these processes and use of these products in e.g. food or feed. Reference is made to the Identification of the Microorganism, having the Identification reference given by the DEPOSITOR of SoF1 and with the Accession number given by the INTERNATIONAL DEPOSITORY AUTHORITY of VTT E-193585. The date of the original deposit is Jun. 11, 2019.