138 results about "Single-cell protein" patented technology

The invention provides a pichia pastoris strain for efficiently converting methanol to produce high-methionine-content single-cell protein and application of the pichia pastoris strain, and belongs to the technical field of biology. According to the invention, a strain of pichia pastoris (named as Pichiapastoris CN-SCP 00 with a preservation number of CCTCC (China Center For Type Culture Collection) NO: M2025657) is obtained through separation, and is subjected to multiple rounds of ARTP iterative mutation breeding, so that a strain Pichiapastoris CN-SCPYE421 (with a preservation number of CCTCC NO: M2025658) for efficiently converting methanol to produce single-cell protein containing high methionine is obtained. Compared with a commercial strain, the Pichiapastoris CN-SCPYE421 has higher methanol tolerance and utilization capacity, the fermentation time is remarkably shortened, and in addition, the Pichiapastoris CN-SCPYE421 can well grow at the temperature of 35 DEG C. The Pichiapastoris CN-SCPYE421 is subjected to fermentation culture in a 5L fermentation tank, the dry weight of thalli reaches 120-150g / L, the protein content reaches 65-75% of the dry weight of the thalli, the Pichiapastoris CN-SCPYE421 is rich in various essential amino acids, and the methionine content reaches 2.3%. The invention provides an excellent strain for industrial development of single-cell protein, and has great social significance and economic value for the feed protein industry.

The invention relates to a Kluyveromyces marxianus strain with high protein yield and application of the Kluyveromyces marxianus strain, which is characterized in that the Kluyveromyces marxianus strain is firstly cultured on a slant, then is transferred into a seed culture medium for seed preparation, and then is inoculated into a fermentation tank, and yeast protein is obtained by adopting index feeding; the bacterial strain is a dominant mutant strain Kluyveromyces marxianus YWX-1, the protein content in a dry thallus reaches 55% or above and is increased by 26% or above compared with that of an original bacterial strain, the bacterial strain can be used for production of single-cell protein, the essential amino acid index (EAAI) value in the protein reaches 1.2, and the bacterial strain is rich in arginine, threonine and lysine and can be applied to production of single-cell protein. These amino acids play a key role in immune regulation, intestinal mucosal barrier function and muscle protein synthesis of young animals. The YWX-1 kluyveromyces marxianus strain has a wide application prospect in the production of neoplasm protein.

The application provides a multi-gas control device, a use method and an application, and relates to the technical field of biological gas fermentation. The device comprises a gas supply module, the gas supply module comprises multiple groups of gas sources and connecting pipelines, each gas source is connected to an air inlet pipeline through a respective connecting pipeline, a secondary pressure reducing valve, a T-shaped filter, a mass flow meter, a stop valve, a regulating valve and a check valve are sequentially arranged on the connecting pipeline, the air inlet pipeline is connected to a liquid circulation pipeline, gas is discharged from a spraying mechanism connected to the liquid circulation pipeline, the spraying mechanism is arranged in a fermentation tank, the spraying mechanism is connected to a circulating pump through the liquid circulation pipeline, the circulating pump is connected to the bottom of the fermentation tank through a pipeline, the top of the fermentation tank is connected to a detection pipeline, and a precision filter, a vacuum pump, a drying device, a pre-analyzer and a gas analyzer are sequentially arranged on the detection pipeline. The application realizes efficient absorption and utilization of multiple gases, and simultaneously obtains high yield and high quality single-cell protein.

The invention discloses a double-carbon-source mixed fermentation metabolism regulation system for single-cell protein production, and belongs to the technical field of fermentation engineering. The method solves the problems that in the prior art, the independent utilization efficiency of methanol and methane is low, the metabolic pathways of microorganisms to the two carbon sources are different, and high-efficiency single-cell protein production is difficult to realize, and by comprehensively analyzing various data related to historical parameters in the historical parameters, the production efficiency of the single-cell protein is improved. According to the method, key metabolic nodes having significant influence on the yield and quality of the single-cell protein can be accurately identified, and reasonable weights are distributed for the key metabolic nodes, so that the production quality of the single-cell protein in fermentation engineering is optimized, and the realization of a production target is ensured; meanwhile, according to the production target of the actual fermentation engineering, the optimization cost of the key metabolism nodes is evaluated, excessive optimization of high-cost nodes is avoided, the economic benefit of single-cell protein production is improved, the cost budget of the fermentation engineering is reduced, and the flexibility of the fermentation engineering and the scientificity of metabolism regulation decision are enhanced.

This disclosure describes methods to separate solids from liquids in a production facility. A process separates components in the process stream by applying non-condensable media to create density differences and then using a mechanical device to separate the solids from the liquids based on the density difference. The process produces the liquids and solids, which may be further processed to create valuable animal feed products.

The invention discloses kluyveromyces marxianus and application of the kluyveromyces marxianus in production of single-cell protein by taking straw as a raw material. The Kluyveromyces marxianus is a ZH-2 bacterial strain which is CGMCC (China General Microbiological Culture Collection Center) No.32295 The strain is obtained from a wild strain in a laboratory adaptive evolution mode, wheat straw and an inorganic nitrogen source can be used as raw materials for efficiently producing single-cell protein, compared with a parent strain, the utilization speed and the utilization rate of biomass, glucose and xylose are remarkably increased, the yield of ZH-2 SCP can reach 0.047 g.g <-1 >, and the yield of ZH-2 SCP can reach 0.045 g.g <-1 >. And the essential amino acid mode basically accords with adult and infant amino acid modes, and can be used as high-quality protein nutrition supply. The Kluyveromyces marxianus ZH-2 strain capable of obtaining single-cell protein by fermenting wheat straw as a raw material is provided from the perspective of utilizing cheap waste resources, so that the production cost of SCP is greatly reduced, and the problem of protein resource shortage is effectively solved.

The present invention relates to a method for producing recombinant proteins, in which a larger cellular morphology is produced by, for example, degrading tags (SsrA16 and SsrA21) by SsrA, regulating morphology-related genes (including but not limited to mreBCD, ftsABEIQWXYZ, sulA, minCDE), thereby enabling more intracellular proteins to be accommodated, thereby improving the production of recombinant proteins (such as MTG and Vip) and single cell proteins.

Food products are provided in which microbial protein sources, such as single cell protein or protein hydrolysis products, are incorporated. Artificial meat products are provided that include microbial protein in place of animal protein.

The present invention discloses a Schizochytrium strain that efficiently converts food waste to produce single-cell protein and its application, belonging to the field of bioengineering technology. The Schizochytrium spLOX‑2 deposit number is: CGMCC No.41623. The present invention also discloses a method for efficiently converting food waste to produce single-cell protein, fermenting food waste to produce volatile fatty acids; and using it as a carbon source to culture Schizochytrium to obtain single-cell protein. The recombinant strain spLOX‑2 in the present invention has improved utilization of lactic acid, and its biomass and protein yields are increased by 14.19% and 12% respectively compared with the wild-type strain, and the oil content is significantly increased by 107.1%. The present invention provides a new path for a sustainable bioeconomic model, in which waste is converted into valuable biochemicals, laying a solid foundation for expanding the scale of the process and the economic feasibility in industrial applications.

The invention discloses candida utilis engineering bacteria and a method for producing single-cell protein by fermenting corn thin pulp, and belongs to the technical field of biology. According to the candida utilis engineering bacteria disclosed by the invention, the candida utilis is subjected to gene modification, a chitin synthase gene in the candida utilis is knocked out, and an exogenous keratinase gene is integrated, so that the candida utilis engineering bacteria can utilize corn thin pulp as a substrate for fermentation. In the fermentation process, carbon and nitrogen sources in the thin corn pulp can be effectively utilized for growth, macromolecular protein in the thin corn pulp can be hydrolyzed and converted into single-cell protein, the content of the single-cell protein in final fermentation liquor is increased, the protein content of the final fermentation liquor can reach 60% or above, and the proportion of the single-cell protein can reach 30% or above; the corn thin pulp can be efficiently utilized, and meanwhile, single-cell protein in the fermentation liquor can be used for adding animal feed so as to meet the requirement for animal protein feed in the market.

The invention relates to the technical field of microbial fermentation, and provides a method for producing single-cell protein, which comprises the following steps: inoculating a crop straw matrix with a first microbial seed for first fermentation; the first microbial seed comprises trametes versicolor; sterilizing a fermentation product obtained by the first fermentation; inoculating second microbial seeds into the sterilized fermentation product for second fermentation; the second microbial seed comprises yarrowia lipolytica / candida utilis. According to the scheme, the combination of the selected first microbial seed solution and the second microbial seed solution has a remarkable synergistic effect, so that the crude protein content, the lignin removal rate, the cellulose retention rate and the yeast biomass are improved, and the hemicellulose loss rate is reduced.

The present application relates to protein derived from microorganisms, and more specifically derived from yeasts, that possess low RNA content and high gelling capacity. This application also relates to an industrial process and industrial uses of such single cell proteins for human and animal nutrition, health and well-being.

The present invention relates to a novel fermentation, extraction and purification process for obtaining a single-cell protein for use in the food industry, using a particular strain of Kluyveromyces marxianus referred to as KMPROTEO 1, which results in a product consisting of a single-cell protein powder with a protein content equal to or greater than 60%, that is highly bioavailable, which, since it is soluble, can be included in different food matrices, without changing the formulation or the profile of the product to which it is added, which we have named "PRO60". The process disclosed in this specification includes the description of the aerobic fermentation of the strain of Kluyveromyces marxianus referred to as KMPROTEO 1, as well as the use of the ingredient or final product obtained, by adding same to some preferred food matrices.

The present application relates to the field of feed raw material crushing device, specifically to a single-cell protein feed raw material processing crushing device, including the crushing tank, the top of the crushing tank is fixedly installed with the driving motor, the lower part of the inner wall of the crushing tank is fixedly connected with the filter plate, the top of the filter plate is rotatably connected with the transmission rod, and the top of the transmission rod is fixedly connected with the rotating end of the driving motor, further including: fiber collection mechanism. The single-cell protein feed raw material processing crushing device adjusts the cooperation of the adjusting ring and the expansion plate, periodically drives the movable blade disc to approach the fixed blade disc, in this process, on the one hand, the fiber wound on the fiber collection rod is pushed into the cutting area, on the other hand, the sharp blades arranged in a staggered manner are used for accurately and efficiently secondary crushing of the fiber, which makes the waste originally affecting production be converted into fine powder and re-mixed into the feed product, thus eliminating the hidden trouble and improving the comprehensive utilization rate of the raw material.

The invention relates to the technical field of waste resource utilization, in particular to preparation of a biogas slurry nanofiltration permeate culture medium and a method for applying the biogas slurry nanofiltration permeate culture medium to continuous production of single-cell protein. The method comprises the following steps: carrying out matrix filtration on biogas slurry obtained by carrying out anaerobic fermentation on livestock and poultry manure, and carrying out membrane filtration on filtrate of the biogas slurry after the matrix filtration; the matrix filtration is enhanced by adopting a coagulant, the membrane filtration is carried out step by step by adopting ultrafiltration and nanofiltration, and the obtained permeate is subjected to C / N and pH regulation. The biogas slurry nanofiltration permeate culture medium provided by the invention is suitable for growth and metabolism of candida utilis, the protein synthesis efficiency is remarkably improved, the nanofiltration permeate is high in purity, interference of infectious microbes and impurities is reduced, the nutritional value and safety of a product are improved, and efficient production of single-cell protein and resource utilization of biogas slurry can be realized.

A Pichia pastoris strain and its application in the fermentation production of single-cell protein belong to the field of microbial fermentation technology. The Pichia pastoris strain... Komagataella phaffii GG7 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 39081, on December 2, 2025. This Pichia pastoris strain GG7 was isolated from soil in a methanol production plant area. It maintains good growth even in high concentrations of methanol (up to 8% (w / v)) and mixed carbon sources such as methanol, acetic acid, and ethanol, achieving a single-cell protein content of approximately 62 wt%. This strain is suitable for aerobic fermentation using methanol, ethanol, and / or acetic acid as carbon sources, exhibiting strong carbon source tolerance and high protein yield, making it suitable for industrial production of single-cell protein.

The present invention relates to an improved method for culturing microorganisms capable of utilizing ethanol as a starting material using continuous culture. The present invention relates to an improved process for the production of biomass, in particular single cell proteins, using continuous culture, wherein the yeast single cell protein product comprises yeast cells of the Saccharomyces cerevisiae; and an animal feed comprising such biomass.

The present application belongs to the field of environmental biotechnology and resource recycling technology, and provides a high-ammonia-nitrogen-tolerant yeast strain and its application in biogas slurry resource treatment. The high-ammonia-nitrogen-tolerant yeast strain is named Torulaspora delbrueckii L12345, which was preserved in the China General Microbiological Culture Collection Center (CGMCC) on January 13, 2026, and the address is No. 1, Yitian West Road, Chaoyang District, Beijing, China Institute of Microbiology, Chinese Academy of Sciences, and the preservation number is CGMCC No. 39123. The present application realizes efficient treatment of biogas slurry and simultaneous production of single-cell protein, reduces the treatment cost, improves the economic benefit, and provides a new technology for clean and high-value utilization of biogas slurry.

A control method and apparatus are provided for the safe operation of aerobic gas fermentation by a C1-fixing microorganism to produce various products such as single cell protein or cultured protein. The aerobic fermentation comprises feeding specific gases (one of which is oxygen) into a bioreactor where they're fermented to products such as single cell protein or cultured protein. Specifically, the control apparatus and method involve measuring the O2 concentration in the headspace above a liquid fermentation broth (in a bioreactor) comprising a C1-fixing microorganism and determining if the concentration is at or below a predetermined set point. Using various sensors, flow controllers and transmitters plus a control system, the flow rate of the O2 comprising gas stream into the bioreactor is adjusted or shut off to maintain the O2 concentration in the headspace below the set point.

The present application relates to the technical field of animal feed, more particularly, to a methanol-based single-cell protein feed composition and a preparation method thereof.The feed composition comprises the following raw material components by weight: 30-70 parts of methanol-based single-cell protein powder, 10-40 parts of plant protein source, 10-30 parts of energy source, 1-10 parts of functional additive, and 5-20 parts of carrier.The present application uses the methanol-based single-cell protein, a non-grain protein source with high protein and good amino acid composition, to significantly improve the palatability, digestibility and nutritional value of the feed by compounding the methanol-based single-cell protein with conventional feed components through a scientific formula and a physical wall-breaking and mixing process, and to partially or completely replace traditional protein sources such as soybean meal, thereby reducing the cost of feed, alleviating the problem of food competition between humans and animals, and providing a sustainable new protein feed solution for the animal husbandry.

Food products are provided in which microbial protein sources, such as single cell protein or protein hydrolysis products, are incorporated, and which contain proteins that are recombinantly expressed in chemoautotrophic microorganisms. Meat, dairy, and egg substitute products are provided that include microbial protein and proteins that are recombinantly produced in chemoautotrophic microorganisms in place of animal protein.

The present disclosure is directed to methods for measuring proteomes of single cells. In one aspect, methods comprise disposing cells on a surface, synthesizing hydrogel chambers around each of the cells, lysing the cells to release intracellular proteins for adsorption onto the surface bounded by the hydrogel chambers, depolymerizing the hydrogel chambers, and quantifying the adsorbed proteins with detection antibodies to determine the single cell proteomes.

The application discloses a single-cell immunoblotting method based on quenchable aptamer probes and relates to the technical field of single-cell protein detection. The method comprises the following steps: S1, a single-cell suspension sample is settled in a microwell array on a composite hydrogel chip; S2, the composite hydrogel chip is placed in an electrophoresis tank, then a lysis solution is slowly poured into the tank, and incubation lysis is carried out; S3, after cell lysis, an electric field is applied to both ends of the chip, and proteins enter a gel coating layer on the surface of the chip under the action of the electric field and start electrophoretic separation; S4, after gel electrophoresis is completed, the chip is placed under an ultraviolet lamp for exposure, and the gel is taken out after exposure is completed; S5, the gel is placed in an aptamer probe solution which is targeted to target proteins, and is soaked and dyed, and the fluorescence signal intensity of the target protein molecules is measured under a laser confocal fluorescence microscope. The application can improve the detection sensitivity of target proteins and has fast imaging speed.

The application provides a method for improving the yield of Cupriavidus necator protein and application, and relates to the technical field of microbial fermentation, and the method comprises the following steps: step 1, sugar fermentation; step 2, gas culture; and step 3, collecting and filtering the bacterial liquid after a set fermentation time. The obtained single-cell protein is used as a zebrafish feed additive, and it is found that the single-cell protein has no side effect on the growth of zebrafish, and can promote the growth of zebrafish and improve the weight gain rate. The single-cell protein has a wide application prospect in the fields of food and feed.

Provided is a method for nucleic acid reduction in a bacterium to less than about 5 wt % of a total dry weight of the bacterium, the method comprising culturing the cells of the at least one bacterium in a fermentation medium to obtain a fermentation medium comprising a biomass; heating the biomass to a temperature of at least 70° C. for at least 10 minutes; and washing the biomass. Also provided are food components, food ingredients and foods or beverages comprising single cell proteins produced from such bacteria.

The present application provides a base model processing method and device for single-cell proteome detection data, which comprises: obtaining single-cell proteome detection data, uniformly processing protein identification information, and standardizing protein abundance data according to detection technology types to obtain uniformly distributed protein expression data; mapping relevant information into identification and abundance embedding vectors respectively and fusing them, adding a special marker vector to construct cell sequence data; inputting the cell sequence data into a base model for pre-training, optimizing parameters through a mask reconstruction and global prediction task to obtain a pre-training model; and fine-tuning the model for a target analysis task, analyzing and processing to-be-processed data to obtain downstream results. The present application can integrate single-cell proteome data of different detection technologies and batches, effectively remove batch effects, extract stable cell low-dimensional representations, support multi-downstream task fine-tuning, realize undetected protein inference and cell trajectory analysis, and improve data utilization efficiency and task adaptability.

The invention belongs to the technical field of synthetic biology and fermentation engineering, and particularly relates to a coupling fermentation method for synthesizing single-cell protein from methanol and carbon dioxide. The coupling fermentation method comprises the following steps: mixing and stirring a fermentation medium and methanol according to a weight part ratio of (97-99): (1-3) to form a fermentation substrate mixed solution; mixing the pre-cultured saccharomycetes liquid, the pre-cultured photosynthetic bacteria liquid and the fermentation substrate mixed liquid according to a weight part ratio of (2-4): (3-6): (90-95), introducing a mixed gas source, and carrying out illumination fermentation to obtain fermentation liquid; and sequentially carrying out centrifugal separation and vacuum drying on the fermentation liquor to obtain single-cell protein. According to the method, the pre-cultured yeast liquid, the pre-cultured photosynthetic bacterium liquid and the fermentation substrate mixed liquid are mixed and then are subjected to aerated fermentation, so that the fermentation capacity of microorganisms at a relatively high fermentation temperature is improved, and the yield of single-cell protein is increased.

This invention provides a method for producing single-cell protein using a mixed bacterial culture, comprising the following steps: performing primary seed culture on methanogenic bacteria, *Tricholoma materata*, and *Glucosinolate methyl-eating bacteria* to obtain primary seed solutions; mixing the primary seed culture solutions evenly to obtain a mixed bacterial solution; inoculating the obtained mixed bacterial solution into a secondary seed culture medium for cultivation to obtain a secondary seed solution; inoculating the obtained secondary seed solution into a fermentation medium for scale-up cultivation to obtain a fermentation broth; separating the solid and liquid components of the obtained fermentation broth, harvesting the bacterial cells, and obtaining single-cell protein. This method for producing single-cell protein using a mixed bacterial culture improves the utilization rate of the carbon source methane and the yield of single-cell protein.

The invention relates to an aeration chamber (2) provided with means (3) for mixing the culture broth contained therein, at least a first gas supply chamber (4) and a second gas supply chamber (5) suitable for containing a first gas and a second gas, respectively, and at least a first gas supply chamber (5) adapted to connect an outlet terminal (7) of the aeration chamber (2) to at least one respective inlet (4a, 4b, 5a, 5b) of each of the first chamber (4) and the second chamber (5), and to introduce the culture broth coming from the aeration chamber (2) into the first gas supply chamber (4) and the second gas supply chamber (5), resulting in the culture broth being mixed with the first gas and the second gas, respectively. The present invention also relates to a process for producing one or more of a dry cell mass or single-cell proteins from CO2.

The invention discloses an integrated feeding control method for acetic acid group single-cell protein fermentation and application of the integrated feeding control method, and belongs to the technical field of microbial fermentation. By monitoring the change of the pH value of the fermentation liquid in real time, the composite material supplementing system containing the carbon and nitrogen sources is automatically triggered, pH signals are converted into the supplementing amount of the carbon sources and the nitrogen sources, the stable carbon-nitrogen ratio of supplemented materials in the fermentation process is maintained, and meanwhile the pH value is maintained in an appropriate range. The thallus yield and the protein content of the single-cell protein are obviously improved; the problems of poor thallus growth, low protein content and large substrate residual quantity caused by mutual independence of a carbon source, a nitrogen source and pH adjustment, tedious control of a fermentation process and imbalance of a carbon-nitrogen ratio in an existing material supplementing mode are solved.