220 results about "Single-cell protein" patented technology

Provided herein are methods identifying protein-protein interactions in a single cell context. In some cases, the methods may be used to measure the abundance of protein-protein interactions in a single cell context. In some cases, the methods may be used to map relationships (e.g., spatial relationships) between proteins in a single cell context. Generally, the methods employ the use of proximity probes coupled with splint oligonucleotides to link information about the relationship of proteins within a single cell context, which may then be read in a downstream process (e.g., a sequencing reaction).

The invention belongs to the technical field of biology, and particularly relates to kluyveromyces marxianus with high beta-glucan content and application of the kluyveromyces marxianus. Specifically, the invention provides a Kluyveromyces marxianus, the classification name of the Kluyveromyces marxianus is Kluyveromyces marxianus CJA0020, and the preservation number of the Kluyveromyces marxianus is CCTCC (China Center For Type Culture Collection) No.M 20241673. According to the Kluyveromyces marxianus screened by the invention, the strain growth speed is high, a strain fermentation product has higher biomass and beta-glucan content, and the beta-glucan content can reach 1.23 + / -0.06 g / L; the biomass can reach 400 g / Kg (wet weight) and the protein content can reach 45% when the strain is cultured for 32 hours during fed-batch fermentation, and the strain can also be used for producing yeast single-cell protein.

The present disclosure relates to single cell protein (SCP) products obtained from Cupriavidus necator that has been engineered to produce digestive enzymes or otherwise enhanced. The biomass produced by the C. necator and the SCP products that include it contain digestive enzymes that are not naturally produced by C. necator.

The invention provides a pichia pastoris strain for efficiently converting methanol to produce high-methionine-content single-cell protein and application of the pichia pastoris strain, and belongs to the technical field of biology. According to the invention, a strain of pichia pastoris (named as Pichiapastoris CN-SCP 00 with a preservation number of CCTCC (China Center For Type Culture Collection) NO: M2025657) is obtained through separation, and is subjected to multiple rounds of ARTP iterative mutation breeding, so that a strain Pichiapastoris CN-SCPYE421 (with a preservation number of CCTCC NO: M2025658) for efficiently converting methanol to produce single-cell protein containing high methionine is obtained. Compared with a commercial strain, the Pichiapastoris CN-SCPYE421 has higher methanol tolerance and utilization capacity, the fermentation time is remarkably shortened, and in addition, the Pichiapastoris CN-SCPYE421 can well grow at the temperature of 35 DEG C. The Pichiapastoris CN-SCPYE421 is subjected to fermentation culture in a 5L fermentation tank, the dry weight of thalli reaches 120-150g / L, the protein content reaches 65-75% of the dry weight of the thalli, the Pichiapastoris CN-SCPYE421 is rich in various essential amino acids, and the methionine content reaches 2.3%. The invention provides an excellent strain for industrial development of single-cell protein, and has great social significance and economic value for the feed protein industry.

The invention relates to a Kluyveromyces marxianus strain with high protein yield and application of the Kluyveromyces marxianus strain, which is characterized in that the Kluyveromyces marxianus strain is firstly cultured on a slant, then is transferred into a seed culture medium for seed preparation, and then is inoculated into a fermentation tank, and yeast protein is obtained by adopting index feeding; the bacterial strain is a dominant mutant strain Kluyveromyces marxianus YWX-1, the protein content in a dry thallus reaches 55% or above and is increased by 26% or above compared with that of an original bacterial strain, the bacterial strain can be used for production of single-cell protein, the essential amino acid index (EAAI) value in the protein reaches 1.2, and the bacterial strain is rich in arginine, threonine and lysine and can be applied to production of single-cell protein. These amino acids play a key role in immune regulation, intestinal mucosal barrier function and muscle protein synthesis of young animals. The YWX-1 kluyveromyces marxianus strain has a wide application prospect in the production of neoplasm protein.

The invention relates to a cell lysis solution, which is prepared from the following active components: 0.08 to 0.2 percent of DDM (Dichloromethane), 35 to 45 mM of CAA (Ceamic Acid Acetic Acid), 5 to 15 mM of TCEP (Trichloroethylene Phosphate), 85 to 100 mM of TEAB (Tetraethyl Ammonium Bromide), 0 to 0.25 percent of Protease MAX and 0 to 0.25 percent of RapiGest SF (SF). The invention further provides a single-cell protein pretreatment method based on the digital microfluidic system, the cell lysis solution is used for lysis, the number of detectable proteins and the number of detectable peptide fragments can be high, and the method can be well used for single-cell proteomics analysis.

A method and apparatus for inoculating a bioreactor with a C1-fixing strain is disclosed. In particular, the present disclosure relates to the cost-efficient methods and apparatus for inoculating a bioreactor with a C1-fixing strain for production of protein, such as single cell protein or cultured protein, by microbial fermentation of a gaseous substrate.

The application provides a multi-gas control device, a use method and an application, and relates to the technical field of biological gas fermentation. The device comprises a gas supply module, the gas supply module comprises multiple groups of gas sources and connecting pipelines, each gas source is connected to an air inlet pipeline through a respective connecting pipeline, a secondary pressure reducing valve, a T-shaped filter, a mass flow meter, a stop valve, a regulating valve and a check valve are sequentially arranged on the connecting pipeline, the air inlet pipeline is connected to a liquid circulation pipeline, gas is discharged from a spraying mechanism connected to the liquid circulation pipeline, the spraying mechanism is arranged in a fermentation tank, the spraying mechanism is connected to a circulating pump through the liquid circulation pipeline, the circulating pump is connected to the bottom of the fermentation tank through a pipeline, the top of the fermentation tank is connected to a detection pipeline, and a precision filter, a vacuum pump, a drying device, a pre-analyzer and a gas analyzer are sequentially arranged on the detection pipeline. The application realizes efficient absorption and utilization of multiple gases, and simultaneously obtains high yield and high quality single-cell protein.

The invention discloses a double-carbon-source mixed fermentation metabolism regulation system for single-cell protein production, and belongs to the technical field of fermentation engineering. The method solves the problems that in the prior art, the independent utilization efficiency of methanol and methane is low, the metabolic pathways of microorganisms to the two carbon sources are different, and high-efficiency single-cell protein production is difficult to realize, and by comprehensively analyzing various data related to historical parameters in the historical parameters, the production efficiency of the single-cell protein is improved. According to the method, key metabolic nodes having significant influence on the yield and quality of the single-cell protein can be accurately identified, and reasonable weights are distributed for the key metabolic nodes, so that the production quality of the single-cell protein in fermentation engineering is optimized, and the realization of a production target is ensured; meanwhile, according to the production target of the actual fermentation engineering, the optimization cost of the key metabolism nodes is evaluated, excessive optimization of high-cost nodes is avoided, the economic benefit of single-cell protein production is improved, the cost budget of the fermentation engineering is reduced, and the flexibility of the fermentation engineering and the scientificity of metabolism regulation decision are enhanced.

The invention provides a fermentation method for producing single-cell protein from corn straw. The fermentation method comprises the following steps: (1) inoculating a microbial seed solution into a crushed corn straw solid culture medium; (2) after the solid-state fermentation is completed, adding water, and reacting at 65-85 DEG C for 4-8 hours; and (3) cooling the liquid culture medium, and then inoculating a microbial seed solution for fermentation. By adopting the method, the yield of single-cell protein can be up to 142.68 g / L, and the yield of crude protein can be up to 44.8%. Compared with direct fermentation of the corn straw, the yield of single-cell protein and the content of crude protein can be remarkably increased, and the fermentation method for achieving efficient utilization of the corn straw is provided.

This disclosure describes methods to separate solids from liquids in a production facility. A process separates components in the process stream by applying non-condensable media to create density differences and then using a mechanical device to separate the solids from the liquids based on the density difference. The process produces the liquids and solids, which may be further processed to create valuable animal feed products.

The invention discloses kluyveromyces marxianus and application of the kluyveromyces marxianus in production of single-cell protein by taking straw as a raw material. The Kluyveromyces marxianus is a ZH-2 bacterial strain which is CGMCC (China General Microbiological Culture Collection Center) No.32295 The strain is obtained from a wild strain in a laboratory adaptive evolution mode, wheat straw and an inorganic nitrogen source can be used as raw materials for efficiently producing single-cell protein, compared with a parent strain, the utilization speed and the utilization rate of biomass, glucose and xylose are remarkably increased, the yield of ZH-2 SCP can reach 0.047 g.g <-1 >, and the yield of ZH-2 SCP can reach 0.045 g.g <-1 >. And the essential amino acid mode basically accords with adult and infant amino acid modes, and can be used as high-quality protein nutrition supply. The Kluyveromyces marxianus ZH-2 strain capable of obtaining single-cell protein by fermenting wheat straw as a raw material is provided from the perspective of utilizing cheap waste resources, so that the production cost of SCP is greatly reduced, and the problem of protein resource shortage is effectively solved.

The invention discloses Candida tropicalis, and the preservation number of the Candida tropicalis in the Guangdong Microbial Culture Collection Center is GDMCC No: 64869. The Candida tropicalis has the advantages that the preservation number of the Candida tropicalis is CGMCC No: 64869; the invention further provides application of the candida tropicalis in fermentation production of single-cell protein, a culture medium suitable for the candida tropicalis and a method for fermentation production of the single-cell protein. The candida tropicalis is a high-stress-resistance strain of vinegar residue hydrolysate, is suitable for large-scale production, can effectively promote recycling of vinegar residue waste biomass resources, and relieves the current situation of protein feed shortage in the breeding industry to a certain extent.

The invention provides a post-treatment method of single-cell protein fermentation liquor and a fermentation method of single-cell protein thalli. Nuclease and cell wall lytic enzyme are respectively added into fermentation liquor containing single-cell protein thalli to treat the thalli, so that nucleic acid and cell wall residues can be reduced, and the problems that in a traditional fermentation process, the application of the thalli in food is limited due to the high nucleic acid content of the thalli, and the protein extraction efficiency is reduced due to the existence of the cell walls are solved. In addition, by optimizing fermentation conditions, the protein content of the single-cell protein is remarkably increased. The process is simple, low in cost, suitable for industrial production and capable of being widely applied to the fields of feed, food and medicine.

The invention provides a methylobacterium strain JC03 for producing single-cell protein and application of the methylobacterium strain JC03 in the field of aquaculture, and belongs to the technical field of microorganisms. The methylobacterium strain JC03 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.32616. The strain is high in growth efficiency, high in thallus crude protein content, rich in amino acid spectrum, high in essential amino acid content and rich in functional components for improving the immunocompetence of aquatic animals; the single-cell protein produced by the strain can completely or partially replace fish meal protein in feeds of marine fishes such as turbot, pseudosciaena crocea, grouper and rainbow trout; vegetable protein in feed of freshwater fish such as grass carp can be completely replaced; animal and plant proteins in feeds for shrimps and crabs, such as penaeus vannamei, can be completely replaced, and animal and plant proteins in stichopus japonicus feeds can be completely replaced; the immunocompetence of fed aquatic animals can be obviously improved. The invention provides a microbial strain for efficiently converting methanol to produce single-cell protein, and the microbial strain has a wide application prospect in the field of aquaculture.

The invention relates to the technical field of feed mixing devices, in particular to a mixing device for Leymus chinensis single-cell protein feed processing, which comprises a stirrer, a stirring mechanism mounted on the stirrer, a dust removal mechanism, a spraying mechanism and a discharging mechanism, a premixing mechanism is mounted on the side surface of the stirrer, and a transmission mechanism is mounted on the dust removal mechanism; when forage is added into the stirrer and in the earlier stage of stirring, large feed dust can be raised, an axial flow fan is connected with an external power supply to suck out the feed dust on the stirrer, the feed dust is collected by a filter screen, clean air is discharged from the tail end of a connecting shell, and a first motor is started to drive a stirring shaft to rotate; the stirring shaft can drive the filter screen to rotate on the connecting shell after rotating, the filter screen can abut against the inner bottom of the connecting shell when rotating anticlockwise, the collected feed dust falls into the connecting hopper along with rotation of the filter screen, collection and treatment of the feed dust are facilitated, and air pollution and feed waste are avoided.

The invention discloses a schizochytrium limacinum strain for efficiently converting kitchen waste to produce single-cell protein and application of the schizochytrium limacinum strain, and belongs to the technical field of biological engineering. The preservation number of the schizochytrium sp LOX-2 is CGMCC (China General Microbiological Culture Collection Center) No. 41623. The invention also discloses a method for producing single-cell protein by efficiently converting kitchen waste. The method comprises the following steps: fermenting food waste to produce volatile fatty acid; and culturing schizochytrium limacinum by using the strain as a carbon source to obtain single-cell protein. According to the recombinant strain spLOX-2, the utilization of lactic acid is improved, the biomass and the protein yield of the recombinant strain spLOX-2 are respectively improved by 14.19% and 12% compared with those of a wild strain, and the grease content is obviously improved by 107.1%. According to the invention, a new way is provided for a sustainable biological economic mode, wastes are converted into valuable biological chemicals, and a solid foundation is laid for expanding the scale of the process and the economic feasibility of the process in industrial application.

The present invention relates to a method for producing recombinant proteins, in which a larger cellular morphology is produced by, for example, degrading tags (SsrA16 and SsrA21) by SsrA, regulating morphology-related genes (including but not limited to mreBCD, ftsABEIQWXYZ, sulA, minCDE), thereby enabling more intracellular proteins to be accommodated, thereby improving the production of recombinant proteins (such as MTG and Vip) and single cell proteins.

Food products are provided in which microbial protein sources, such as single cell protein or protein hydrolysis products, are incorporated. Artificial meat products are provided that include microbial protein in place of animal protein.

The invention discloses a separation method of single-cell protein fermentation liquor. The separation method comprises the following steps: 1) adding a filter aid into fermentation liquor containing single-cell protein thalli to form mixed liquor; 2) filtering the mixed solution through a plate frame to form a filter cake; 3) introducing nitrogen to purge the filter cake to remove moisture in the filter cake; and 4) carrying out flash evaporation drying on the dewatered filter cake to obtain a single-cell protein product. According to the process, filter cakes are formed through plate and frame filtering and squeezing, and then single-cell protein thalli are obtained through a flash evaporation drying technology. The process not only improves the extraction efficiency of the single-cell protein, but also reduces the production cost, and is suitable for large-scale production.

The present invention discloses a Schizochytrium strain that efficiently converts food waste to produce single-cell protein and its application, belonging to the field of bioengineering technology. The Schizochytrium spLOX‑2 deposit number is: CGMCC No.41623. The present invention also discloses a method for efficiently converting food waste to produce single-cell protein, fermenting food waste to produce volatile fatty acids; and using it as a carbon source to culture Schizochytrium to obtain single-cell protein. The recombinant strain spLOX‑2 in the present invention has improved utilization of lactic acid, and its biomass and protein yields are increased by 14.19% and 12% respectively compared with the wild-type strain, and the oil content is significantly increased by 107.1%. The present invention provides a new path for a sustainable bioeconomic model, in which waste is converted into valuable biochemicals, laying a solid foundation for expanding the scale of the process and the economic feasibility in industrial applications.

The present invention relates to a method to prepare a high-protein (microbial) biomass or SCP from an alcohol feedstock. In particular, it is an object to provide a method for preparing such a high-protein (microbial) biomass wherein the oxygen transfer capacity is improved. The invention is directed to a method for the production of biomass, in particular single cell protein, wherein the yeast single cell protein product comprises Saccharomycetales yeast cells, as well as to an animal feed comprising such biomass.

The invention discloses candida utilis engineering bacteria and a method for producing single-cell protein by fermenting corn thin pulp, and belongs to the technical field of biology. According to the candida utilis engineering bacteria disclosed by the invention, the candida utilis is subjected to gene modification, a chitin synthase gene in the candida utilis is knocked out, and an exogenous keratinase gene is integrated, so that the candida utilis engineering bacteria can utilize corn thin pulp as a substrate for fermentation. In the fermentation process, carbon and nitrogen sources in the thin corn pulp can be effectively utilized for growth, macromolecular protein in the thin corn pulp can be hydrolyzed and converted into single-cell protein, the content of the single-cell protein in final fermentation liquor is increased, the protein content of the final fermentation liquor can reach 60% or above, and the proportion of the single-cell protein can reach 30% or above; the corn thin pulp can be efficiently utilized, and meanwhile, single-cell protein in the fermentation liquor can be used for adding animal feed so as to meet the requirement for animal protein feed in the market.

The present invention relates to a composition comprising yeast single cell protein (SCP) product comprising at least about 14% (w / w) essential amino acids per gram of dry yeast SCP product. Preferably, the yeast single cell protein product is produced utilizing ethanol or molasses as a feedstock. The yeast single cell protein product contains advantageously a high ratio of unsaturated versus saturated fatty acids.

The invention relates to the technical field of biology, in particular to a method for increasing the growth rate of a Cupriavidus hookworm strain with carbon dioxide as a carbon source and an engineering strain. Glucokinase genes participating in glucose metabolism in the Cupriavidus hookworm strain are knocked out, alkaline phosphatase genes and haloid acid dehalogenase family (HAD) phosphatase genes are knocked out on the basis, and the growth rate of the Cupriavidus hookworm strain under the condition that carbon dioxide serves as a carbon source is increased through the operation. The bacterial strain with the increased proliferation rate can be used for producing single-cell protein by taking carbon dioxide as a raw material and is beneficial to fixation of greenhouse gas carbon dioxide. Meanwhile, the production efficiency can be remarkably improved by utilizing the chassis cell to construct a metabolic engineering-based product synthesis strain.

The invention relates to the technical field of microbial fermentation, and provides a method for producing single-cell protein, which comprises the following steps: inoculating a crop straw matrix with a first microbial seed for first fermentation; the first microbial seed comprises trametes versicolor; sterilizing a fermentation product obtained by the first fermentation; inoculating second microbial seeds into the sterilized fermentation product for second fermentation; the second microbial seed comprises yarrowia lipolytica / candida utilis. According to the scheme, the combination of the selected first microbial seed solution and the second microbial seed solution has a remarkable synergistic effect, so that the crude protein content, the lignin removal rate, the cellulose retention rate and the yeast biomass are improved, and the hemicellulose loss rate is reduced.

The present invention belongs to the technical field of environmental protection, and specifically relates to a system and method for the co-metabolism of methane-oxidizing bacteria and microalgae to produce microbial protein from biogas, including a methane-oxidizing bacteria culture system, a microalgae culture system, and a first-stage microbial protein cultivation system, a second-stage microbial protein cultivation system, a microbial protein nanofilm filtration system, and a microbial protein vacuum drying system that are sequentially connected. The methane-oxidizing bacteria culture system is connected to the first-stage microbial protein cultivation system through the first methane-oxidizing bacteria liquid discharging system and to the second-stage microbial protein cultivation system through the second methane-oxidizing bacteria liquid discharging system. The microalgae culture system is connected to the first-stage microbial protein cultivation system through the first microalgae culture liquid discharging system and to the second-stage microbial protein cultivation system through the second microalgae culture liquid discharging system. In the present invention, methane and carbon dioxide in the biogas generated by the anaerobic fermentation of food waste are metabolized by methane-oxidizing bacteria and microalgae, thereby producing microbial protein.

The present application relates to protein derived from microorganisms, and more specifically derived from yeasts, that possess low RNA content and high gelling capacity. This application also relates to an industrial process and industrial uses of such single cell proteins for human and animal nutrition, health and well-being.

The present invention is applicable to the field of fermentation engineering technology, and provides a biological reaction device and process for producing single-cell protein by methanotrophs. The biological reaction device for producing single-cell protein by methanotrophs includes: a tank for methanotrophs to ferment methane to produce single-cell protein, and a tank cover is installed on the top of the tank; a stirring assembly for stirring the mixture in the tank, and the stirring assembly is arranged in the tank; a ventilation assembly installed on the tank cover for transporting methane and air into the tank; and a steam disinfection and sterilization assembly for sterilizing the inside of the tank with steam before production. The biological reaction device for producing single-cell protein by methanotrophs provided by this solution combines gas transportation and stirring, simplifies the equipment, and has a steam disinfection and sterilization function, which can comprehensively disinfect the inside of the tank and is relatively convenient to use.

The present invention relates to a novel fermentation, extraction and purification process for obtaining a single-cell protein for use in the food industry, using a particular strain of Kluyveromyces marxianus referred to as KMPROTEO 1, which results in a product consisting of a single-cell protein powder with a protein content equal to or greater than 60%, that is highly bioavailable, which, since it is soluble, can be included in different food matrices, without changing the formulation or the profile of the product to which it is added, which we have named "PRO60". The process disclosed in this specification includes the description of the aerobic fermentation of the strain of Kluyveromyces marxianus referred to as KMPROTEO 1, as well as the use of the ingredient or final product obtained, by adding same to some preferred food matrices.