The invention discloses a
rare cell detection
chip and an application thereof. The
chip comprises a matrix and a substrate, wherein the matrix is divided into an upper layer and a lower layer; a
rare cell screening membrane is integrated between the upper layer and the lower layer; a sample inlet, a sample outlet and a sample channel are formed in each of the upper layer and the lower layer, which are suitable for the control of micro fluid such as blood samples, buffer solutions,
cell culture solutions, other biochemical reagents and the like. When a sample is input from the sample inlet in the upper layer, and is output from the sample outlet in the lower layer, rare cells in the sample can be selectively blocked at the upper end of the
cell screening membrane. According to the need, the buffer solutions, the
cell culture solutions or other biochemical reagents are input from the sample inlet in the upper layer or the sample inlet in the lower layer, and are output from the sample outlet in the upper layer or the sample outlet in the lower layer so as to realize various cell operations and analysis and corresponding
molecular biology detection. By adopting the
rare cell detection
chip disclosed by the invention, the problem of poor selectivity and sensitivity in capture of rare cells is solved; and the rare cell detection chip is suitable for efficient capture, culture and analysis of circulating
tumor cells in the
peripheral blood of the
human body and fetal circulating cells.