395 results about "Cryopreservation" patented technology

The utility model relates to the field of experimental consumables, in particular to a cryopreservation tube protective cover and a cryopreservation tube. The cryopreservation tube protection cover is matched with a tube body and a cover body of a cryopreservation tube and comprises a shell and a separation membrane. The housing has a first section and a second section opposite the first section. The first section of the shell is matched with the pipe body and connected with the pipe body in a sealed mode. And the second section of the shell is matched with the cover body and is detachably connected with the cover body. The edge of the separation film is connected to the inner wall of the shell and used for closing the opening of the pipe body. The cryopreservation tube comprises the tube body, a cover body and a protective cover. Whether the cryopreservation tube is opened for the first time or not is judged by judging whether the separation membrane is punctured and damaged or not, and the uniqueness function of a biological sample can be achieved easily. A sample is protected by the separation membrane after the cover body is opened and before sampling, so that the cleanliness of the sample is improved.

The present invention provides preparations of MSCs with important therapeutic potential. The MSC cells are non-primary cells with an antigen profile comprising less than about 1.25% CD45+ cells (or less than about 0.75% CD45+), at least about 95% CD105+ cells, and at least about 95% CD166+ cells. Optionally, MSCs of the present preparations are isogenic and can be expanded ex vivo and cryo-preserved and thawed, yet maintain a stable and uniform phenotype. Methods are taught here of expanding these MSCs to produce a clinical scale therapeutic preparations and medical uses thereof.

Methods, systems, and compositions are provided for extracting bone marrow cells from bone obtained from deceased donors, for preparing the bone marrow for cryopreservation, and for obtaining desired cells from cryopreserved and fresh bone marrow

Some embodiments relate to improved cryopreservative formulations and improved methods for cryopreserving and thawing cells. These formulations facilitate high density, low volume cell cryopreservation strategies which overcome the need for washing of cells post-thaw, and therefore result in greater cell recovery and viability. Pharmaceutical compositions comprising a cell population and a cryopreservative formulation as provided herein are also provided. Methods for treating a subject having a disease or condition that benefits from transplantation of a cryopreserved and thawed cell population are also provided.

The invention relates to the field of cells, in particular to a cell culture method which comprises the following steps: S1, taking out a cryopreservation tube from liquid nitrogen; s2, quickly putting the mixture into a water bath, and shaking the mixture from time to time to quickly melt the mixture; s3, wiping and disinfecting a cryopreservation tube with alcohol, opening a cover, injecting the cell suspension into a centrifugal tube by using a suction tube, dropwise adding a culture solution, and carrying out low-speed centrifugation; s4, pouring out the supernate, and adding a culture solution to suspend the cells; s5, sucking the cells into a culture dish filled with a culture solution, and slightly shaking all around, so that the cells in the culture dish are uniformly distributed; s6, placing the culture dish in an incubator at 37 DEG C for culturing; and S7, during culture, replacing a culture solution in the culture dish through a solution replacement mechanism arranged on the culture box in a closed state of the culture box. According to the invention, the culture solution of the culture dish can be replaced when the culture box is closed.

The utility model relates to the technical field of cell cryopreservation, in particular to a cell cryopreservation box matched with straw, which comprises a cell cryopreservation box, the cell cryopreservation box comprises a bottom box and a box cover, the bottom box and the box cover are arranged in a rectangular structure, and the box cover is matched and buckled on the bottom box. A plurality of spacing columns used for spacing straws are uniformly arranged in the bottom box, a plurality of liquid nitrogen inlet grooves used for liquid nitrogen to enter are formed in the middle of the interior of the bottom box in a penetrating mode, protruding blocks are arranged on the outer walls, close to the top corners of the periphery, of the bottom box, the protruding blocks are of an L-shaped structure, and when the box cover is buckled on the bottom box, the bottom of the box cover abuts against the tops of the protruding blocks; compared with the prior art, the cell cryopreservation box can store a large number of straws, the sealed straws can be placed in the cell cryopreservation box, the operation process is simplified, additional materials such as circular thumb tubes, low-temperature storage aluminum strips and special liquid nitrogen tanks are not needed, the operation difficulty and cost are reduced, and the purposes that the straws are convenient to place, observe and operate are achieved.

The present invention provides means with which bile acids, phenols, indoles and organic acids contained in a sample can be stably preserved without cryopreservation of the sample. A sample preservation solution of the present invention is a sample preservation solution used for analyzing at least one component selected from the group consisting of bile acids, phenols, indoles and organic acids contained in a biological or environmental sample, containing at least the following (A) and (B): (A) condensed phosphate or polyoxyethylene sorbitan alkylate; and (B) guanidinium thiocyanate, Tris-HCl (pH 7 to 9), and EDTA.

The utility model belongs to the field of cryopreservation tubes, and discloses a biological sample cryopreservation tube with an RFID (Radio Frequency Identification Device) electronic label, which comprises a tube body, the tube body is of a hollow structure with two open ends, and an interlayer is fixedly connected inside the tube body and divides the internal space of the tube body into two drawing cavities; the drawer box is detachably inserted into the drawing cavity, one end of the drawer box is fixedly connected with a connecting sleeve, a sliding groove is formed in the side wall of the connecting sleeve, a pushing rod is slidably inserted into the connecting sleeve, the inner side end of the pushing rod is fixedly connected with a connecting rod, and the other end of the connecting rod penetrates through the sliding groove to be fixedly connected with the sliding rod; the outer side end of the push rod is fixedly connected with a pressing plate, and the peripheral side of the push rod is sleeved with a spring. And the tube cover is rotationally connected to the connecting sleeve, and the tube cover is detachably connected with the end part of the tube body. The internal space of the tube body is divided into the two drawing cavities by the interlayer, the drawer box can be mounted in each drawing cavity, and the tube cover is connected with the end part of the tube body, so that the utilization rate of the internal space of the tube body is greatly improved.

The utility model discloses a multi-stage dehydration device for stem cell vitrification cryopreservation, which comprises a base, the top of the base is provided with a containing groove, the containing groove is internally provided with a storage pipe for storing stem cell suspension and a [-shaped frame, the top of the [-shaped frame is provided with a lifting assembly, and the lifting assembly is provided with a water inlet and a water outlet. The movable end of the lifting assembly is fixedly provided with an injection pipe, the outer wall of the injection pipe is fixedly communicated with a peristaltic pump through a pipeline, through linkage control of the lifting assembly and the peristaltic pump, automatic conveying of a dehydrating agent and accurate lifting of the injection pipe are achieved, manual pipetting or container replacement is not needed, the operation efficiency is remarkably improved, and the labor intensity of workers is reduced. The quick plugging design of the quick-plugging male connector and the quick-plugging female connector supports switching of multiple groups of dehydrating agent storage tanks as required, ensures continuous gradient progression of dehydrating agents with different concentrations, realizes gradual dehydration of stem cell suspension in a gradient concentration environment, realizes smooth transition of the concentration of the dehydrating agent, and is beneficial to further improvement of the survival rate of stem cells.

This invention provides a dry ice transport insulated box for cell products, belonging to the technical field of transport boxes. It includes a box body and lid secured by latches. Inside the box, partitions separate storage compartments for cryopreservation bags, cryopreservation tubes, and dry ice. The dry ice storage compartments are located around the cryopreservation bag and cryopreservation tube storage compartments. Two thermometers are fixed to the side of the box body, with their probes extending into the cryopreservation bag and cryopreservation tube storage compartments respectively, allowing real-time monitoring of the box's internal temperature. The cryopreservation bag storage compartment is used to store strip-shaped cryopreservation boxes containing cryopreservation bags, while the cryopreservation tube storage compartment is used to store square cryopreservation boxes containing cryopreservation tubes. This invention isolates the storage compartments for each cell, preventing collisions and impacts on cell quality stability during transport.

Methods for cryopreservation of biological samples are provided. The biological samples are sub-millimeter or millimeter scale biological materials. The cryopreservation system includes the use of a conduction-dominated cryomesh (CondD-C). The methods include loading the CondD-C with biological samples and submerging the CondD-C with the samples in a manner, e.g., by vertical plunging, that removes the vapor bubbles from the cooling surfaces. Methods using the CondD-C and vertical plunging can enhance the cooling rate and warming rates and are scalable for high throughput processing of large numbers of vitrified and / or rewarmed biological samples.

The invention provides a biological cell freezing visualization system and method based on a metal woven belt, and relates to the technical field of biologication.The biological cell freezing visualization system comprises a bottom plate, a support platform, a profound hypothermia liquid tank and an observation assembly are fixedly connected to the upper surface of the bottom plate, a lifting sliding table is fixedly connected to the inner bottom face of the support platform, and a constant hypothermia copper table is fixedly connected to the upper end of the lifting sliding table; the constant hypothermia copper table is communicated with the deep hypothermia liquid tank through a liquid conveying hose, the inner top surface of the support platform is fixedly connected with a woven belt fixing support and a sample placing platform, one end of a metal woven belt is fixedly connected to the lower surface of the woven belt fixing support, and the other end of the metal woven belt is fixedly connected to the lower surface of the sample placing platform; a cover plate covers the sample placing platform, and the observation assembly is used for shooting an image of the sample placing platform. Cold is conducted through the metal woven belt, the requirement for the movement precision of the device is low, the durability of the device is improved, the position of the sample containing platform is fixed, and it is guaranteed that imaging of the observation assembly is clear.

The utility model discloses a cryopreservation tube sorting device, and belongs to the technical field of biological experiment equipment. A cryogenic vial sorting device comprises a bottom frame, a sorting box is installed on the bottom frame, a plurality of sorting holes for sorting cryogenic vials are formed in the bottom wall of the sorting box, a push plate is arranged between the bottom frame and the sorting box, sliding grooves are formed in the two sides of the bottom frame respectively, and the push plate is arranged in the sliding grooves. Thrust seats located in the pair of sliding grooves respectively are installed at the two ends of the push plate, and locking pieces are arranged between the fixing seat and the thrust seats. According to the cryopreservation tube sorting device, by means of the ingeniously-designed sorting holes and push plate structures, a user can easily and accurately sort cryopreservation tubes, the special design of the sorting holes ensures that only the cryopreservation tubes meeting specific conditions (for example, the cryopreservation tubes are upright but not inverted) can pass through, the sorting accuracy is greatly improved, and meanwhile the sorting efficiency is greatly improved. And due to the design of the push plate, the cryopreservation tubes can be quickly pushed upwards after sorting is completed, the sorting process is simplified, and the overall efficiency is improved.

The utility model provides a cell cryopreservation bag. The cell cryopreservation bag comprises a bag body, a sample introduction part and a sampling part, the bag body comprises a plurality of communicated cavities, and a channel is formed between every two adjacent cavities so that the adjacent cavities can be communicated. The sample introduction part comprises a sample introduction port and an air sample introduction pipe connected with the sample introduction port, and the air sample introduction pipe is used for being connected with the external atmosphere so that the air pressure in each cavity can be kept consistent with the external atmospheric pressure. The sampling part is arranged on the bag body and corresponds to the cavity. According to the cell cryopreservation bag, the air sample injection pipe is arranged on the sample injection part, and the channels are formed between the cavities, so that the cavities can be communicated with the outside atmosphere after sample injection is completed, cell suspension samples can be automatically and uniformly subpackaged into the cavities, the subpackaging uniformity of the cell suspension samples is improved, and the subpackaging efficiency of the cell suspension samples is improved. Manual extrusion of an operator is not needed, so that the operation is convenient, the time is shortened, and the cell cryopreservation efficiency is improved.

The invention relates to the technical field of sheep semen storage, and discloses a sheep fresh semen low-temperature long-acting preservation diluent containing indole-3-pyruvic acid, the sheep fresh semen low-temperature long-acting preservation diluent containing indole-3-pyruvic acid comprises a basic diluent and an additive, the additive is indole-3-pyruvic acid, and the concentration of the additive in the diluent is 15 mg / L-35 mg / L. The basic diluent comprises the following components: 3.1 g of glucose; 1.4 g of sodium citrate; 20 mL of yolk; 100 [mu] L of lincomycin hydrochloride; 0.50 g of bovine serum albumin; and 0.50 g of vitamin C. The preparation method of the basic diluent comprises the following steps: dissolving glucose, sodium citrate, bovine serum albumin and vitamin C in distilled water, oscillating, filtering and sterilizing, adding yolk and lincomycin hydrochloride, uniformly mixing to obtain the basic diluent, and sealing and storing at 4 DEG C. According to the sheep fresh sperm low-temperature preservation diluent containing indole-3-pyruvic acid provided by the invention, 25 mg / L of indole-3-pyruvic acid is added into the diluent, so that the preservation quality and time efficiency of sheep sperms under the low-temperature condition of 4 DEG C can be improved.

The utility model relates to the technical field of sample storage, and provides a sample storage device which comprises a storage shelf, a cryopreservation box bracket and a manipulator, and an access opening is formed in one side of the storage shelf; the multiple cryopreservation box brackets are arranged in the storage shelf at intervals in the height direction of the storage shelf, and dragging grooves are formed in the sides, close to the storing and taking openings, of the cryopreservation box brackets; the mechanical arm comprises a towing hook capable of moving on the side, close to the storing and taking opening, of the storage goods shelf, and the towing hook is used for being embedded into the dragging groove so as to push and pull the corresponding cryopreservation box bracket. The storage density of the sample storage device can be improved, and meanwhile, the convenience of sample access is kept.

The invention belongs to the technical field of biology, and particularly discloses a chicken semen cryopreservation solution and a preparation method thereof. The chicken semen cryopreservation liquid comprises a cryoprotection liquid I and a cryoprotection liquid II. The freezing protection liquid I is prepared from the following components: glucose, sodium glutamate, potassium acetate, dipotassium phosphate, magnesium acetate, N, N-bis (2-ethoxyl)-2-aminoethanesulfonic acid and an antibiotic solution. And the freezing protection liquid II comprises dimethylformamide and nano zinc oxide. The invention discloses a chicken semen cryopreservation solution and a preparation method thereof, by applying the chicken semen cryopreservation solution, the fertilization and incubation performance of frozen semen is remarkably improved, centrifugation-free and direct semen deposition of the frozen semen are realized, and physical damage and activity reduction caused by the centrifugation process to sperms are avoided.

The utility model discloses a low-temperature preservation device for mixed solution, which comprises a device body and a box door, a square plate is embedded in the device body, a frame body is arranged on the surface of the square plate, a filter screen is embedded on the surface of the frame body, absorbent cotton is embedded in the frame body, and a fixing block on the surface of the frame body is connected with a fixing rod. The fixing rods are symmetrically distributed, and buckles are embedded in the device body. According to the low-temperature storage device for the mixed solution, a square plate is embedded in the device body, before the solution needs to be placed, a frame body is firstly installed on the surface of the square plate, the frame body is installed on the square plate in a sliding mode, at the moment, a fixing rod is clamped into a buckle, and then the frame body can be fixed; at the moment, the container filled with the mixed solution can be easily placed on the filter screen on the surface of the frame body, and the fused solution attached to the outer wall of the container can directly drop on the absorbent cotton through the filter screen, so that the liquid is effectively prevented from flowing around, and the container is relatively convenient to use.

The invention provides an injection for inducing mesenchymal stem cells. The induced mesenchymal stem cell injection is prepared from induced mesenchymal stem cells modified by hepatocyte growth factors with the cell density being 1.0 * 10 < 5 >-2.0 * 10 < 7 > / ml, HSA with the mass concentration being 0.25%-6%, DMSO with the mass concentration being 5%-10%, glucose with the mass concentration being larger than 0 and smaller than 1%, compound amino acid with the concentration being 0.5-5 mg / mL and the balance compound electrolyte injection. And the induced mesenchymal stem cell injection has a pH value of 6.8 to 7.5. The induced mesenchymal stem cell injection is good in homogeneity, small in batch difference and stable in curative effect, can effectively promote damaged tissue repair, can be directly intravenously injected after cryopreservation and recovery, keeps high cell viability and drug activity, achieves an excellent treatment effect, has good room-temperature stability, is convenient to transport and store, and is suitable for clinical application. The method is suitable for different clinical application occasions, and the clinical application range of the mesenchymal stem cells is expanded.

The present disclosure provides a cell patch cryopreservation solution, which includes: a cryoprotectant, a buffer, a reducing agent, a calcium channel inhibitor and an apoptosis inhibitor. The present disclosure also provides a method of cryopreserving a cell patch by using the aforementioned cell patch cryopreservation solution. The cell patch cryopreservation solution and the method of cryopreserving a cell patch provided by the present disclosure can maintain the structural integrity and the cell activity of the cell patch.

1. Name of the product in this design: Packaging Box (Cryopreservation Solution 1). 2. Application of this design: This design is used for packaging boxes of cryopreservation solutions. 3. The key design features of this product are the combination of shape and pattern. 4. The image or photograph that best illustrates the design highlights: 3D view of component 1. 5. The right view of component 1 is a standard design, so the right view of component 1 is omitted; the top view of component 2 is a standard design, so the top view of component 2 is omitted; the bottom surface of this product is a part that is not easily seen or cannot be seen during use, so the bottom view of component 2 is omitted; the rear view of component 2 is the same as the front view of component 2, so the rear view of component 2 is omitted; the right view of component 2 is the same as the left view of component 2, so the right view of component 2 is omitted.

The utility model provides a cell freezing storage device. The cell freezing storage device comprises a storage tank and a tank cover, an upper cover plate is arranged at the upper end in the storage tank; a supporting column is rotationally arranged in the middle of the inner bottom of the storage tank, multiple sets of test tube fixing pieces are evenly arranged on the outer side wall of the supporting column in the circumferential direction, and each set of test tube fixing piece comprises an upper fixing ring fixed to the outer wall of the supporting column and a lower fixing support slidably connected with the outer wall of the supporting column; pull rings are evenly arranged on the surface of the supporting column, the lower end of each pull ring is connected with a traction piece, and the traction pieces are slidably arranged in the supporting column in a penetrating mode and can drive the second supporting rods at the lower ends of the traction pieces to slide up and down. According to the utility model, an operator can conveniently take a single test tube without influencing the cryopreservation state of other test tubes; test tubes can be taken and placed on the upper cover plate, cells are convenient to take and place, and an operator does not directly contact a cell storage area, so that the operator is effectively prevented from being frostbitten; when an operator takes and places the cells, the operator does not directly contact with the cell storage area, so that bacteria can be prevented from being generated in the cell storage area.

The application relates to a preparation method and application method of a mesenchymal stem cell protective agent composition, and relates to the technical field of stem cell treatment, in particular to a protective agent composition, a preparation technology of the protective agent composition and an application technology of the protective agent composition in mesenchymal stem cell treatment. The application solves the problems of insufficient cell stemness maintenance, complex formula or cell toxicity in the existing mesenchymal stem cell cryopreservation. The protective agent composition prepared by the preparation method comprises a deferoxamine pretreatment protective agent and a sodium lactate Ringer injection storage solution. The application method inhibits low-temperature-induced ferroptosis through DFO pretreatment, so that the mesenchymal stem cells can still maintain the multi-directional differentiation potential, the immunoregulatory function and the proliferation ability after being treated at 4 DEG C for 24-48 hours, the serum or complex nutritional ingredients do not need to be added, and the protective agent composition has the advantages of clear composition, high safety, simple operation and the like. The application is suitable for the fields of stem cell treatment, regenerative medicine, pet medical treatment and the like.

The utility model discloses a multi-layer type exosome cryopreservation device which comprises a tank type shell and a nozzle sleeve arranged at the top end of the tank type shell, a tank nozzle is fixed in the nozzle sleeve, an inner container is arranged in the center of the tank type shell, and the bottom end of the inner container is fixedly connected with the inner wall of the bottom end of the tank type shell through a plurality of heat insulation fixing supports. The multiple heat insulation fixing supports can enable the inner container to be fixed in the tank type shell in a suspended mode, the novel lifting and protection device is additionally arranged outside the tank type shell, the lifting and protection device can achieve the protection effect through the multiple arc-shaped metal protection plates surrounding the tank type shell, and during the transportation period of the multi-layer exosome cryopreservation device, the multiple arc-shaped metal protection plates surround the tank type shell. A reliable anti-collision barrier can be formed, the risk that the device is damaged due to collision is greatly reduced, the safety of the device is effectively protected, a heat insulation pad on the inner wall of the device can remarkably reduce interference of external temperature, a stable low-temperature environment in the device can be maintained, and the cryopreservation effect of exosomes is guaranteed.

The utility model provides a cryopreservation box which comprises a shell and a baffle, and the baffle is arranged in the shell and divides the interior of the shell into different layers from bottom to top. A notch is formed in the baffle plate; the gaps are used for communicating different layers to form a flow channel; a low-temperature cooling object is placed in the flow channel and can flow in the flow channel; cryopreservation tube placing holes are formed in the shell and the baffle, and the cryopreservation tube placing holes in the shell correspond to the cryopreservation tube placing holes in the baffle in a one-to-one mode. According to the utility model, the interior of the shell is designed into different layers and is matched with the notches to form a flow channel, so that a low-temperature cooling object in the shell can fully flow and circulate, even if dry ice in contact with the bottom of the tube sublimates, the dry ice at other positions can flow to the position, and the continuous low-temperature state of stem cells is ensured.

Provided are a T cell culture medium, a cell culture, a culture method therefor, and an application. The culture medium comprises IL7 and TGFβ. A method for obtaining T cells comprises contacting immune cells with a culture medium, and resulting CD4 / CD8 positive mixed T cells and universal T cell cultures have high cell proliferation capacity, viability and in vivo efficacy after cryopreservation and thawing.

The application relates to the technical field of storage equipment, and particularly provides a filling device for cryopreservation tubes, which aims to solve the technical problem of low filling efficiency in the prior art when the cryopreservation tubes are filled into a cryopreservation tube sleeve. To this end, the filling device comprises a fixing member, a tube rotating device, a tube pushing device and a limiting device which are mounted on the fixing member, the fixing member is provided with a containing cavity, a lower tube cavity and a tube pushing cavity which are communicated with the containing cavity, the tube rotating device is provided with a tube rotating cavity, the tube rotating device is located in the containing cavity and can move in the containing cavity so that the tube rotating device can be switched between a first working position and a second working position, when the tube rotating device is located in the first working position, the tube rotating cavity is communicated with the lower tube cavity, when the tube rotating device is located in the second working position, the tube rotating cavity is communicated with the tube pushing cavity, the limiting device can fix the cryopreservation tube sleeve at a first end of the tube pushing cavity, and the tube pushing device can push the cryopreservation tube transported into the tube pushing cavity into the cryopreservation tube sleeve from a second end of the tube pushing cavity.

The utility model discloses a one-time code scanning mechanism for the inside and outside of a cryogenic vial storage box, which is characterized in that a cryogenic vial storage box placing station is formed on the upper end face of an outer shell, and a code scanning light-transmitting plate is arranged at the cryogenic vial storage box placing station; a first light reflecting assembly is arranged at the position, close to the side portion of the cryopreservation tube storage box placing station, of the rear side of the outer shell. A first code scanning device is arranged at the position, below the first light reflecting assembly, in the outer shell. A second light reflecting assembly is arranged at the position, below the code scanning light-transmitting plate, in the outer shell, and a second code scanning device is arranged at the position, located on one side of the second light reflecting assembly, in the outer shell; and the first code scanning equipment and the second code scanning equipment are used for scanning codes of the cryogenic vial storage boxes at the cryogenic vial storage box placing station together. According to the utility model, the two-dimensional bar code at the side part of the cryogenic vial storage box and the two-dimensional bar code at the bottom of the cryogenic vial can be scanned simultaneously, the structure is simple, the first reflective assembly and the second reflective assembly can reduce the depth-of-field distance of code reading, and the occupied space is small.

1. The name of the design product: cryopreservation bag cell recovery device. 2. The use of the design product: device for recovering cells in a cryopreservation bag. 3. The design features of the design product: in shape. 4. The picture or photo that best indicates the design features: perspective view.