655 results about "Cryopreservation" patented technology

The utility model relates to the field of experimental consumables, in particular to a cryopreservation tube protective cover and a cryopreservation tube. The cryopreservation tube protection cover is matched with a tube body and a cover body of a cryopreservation tube and comprises a shell and a separation membrane. The housing has a first section and a second section opposite the first section. The first section of the shell is matched with the pipe body and connected with the pipe body in a sealed mode. And the second section of the shell is matched with the cover body and is detachably connected with the cover body. The edge of the separation film is connected to the inner wall of the shell and used for closing the opening of the pipe body. The cryopreservation tube comprises the tube body, a cover body and a protective cover. Whether the cryopreservation tube is opened for the first time or not is judged by judging whether the separation membrane is punctured and damaged or not, and the uniqueness function of a biological sample can be achieved easily. A sample is protected by the separation membrane after the cover body is opened and before sampling, so that the cleanliness of the sample is improved.

The invention discloses hydrogel bio-ink, a preparation method of the hydrogel bio-ink, a 3D printing biodegradable airway stent and application of the 3D printing biodegradable airway stent. The preparation method comprises the following steps that S1, lung tissue is subjected to freeze thawing, slicing, sequential decellularization of SDS and TritonX-100, air drying, smashing and cryopreservation, and a lung extracellular matrix is obtained; s2, performing sterilization and pepsin treatment on the lung extracellular matrix prepared in S1 to obtain a pretreated lung extracellular matrix; and S3, mixing methyl propionylated gelatin and an alginate solution, pouring the mixture into solid gel, gelatinizing the solid gel, and adding the pretreated lung extracellular matrix to prepare the hydrogel bio-ink. The prepared pig lung acellular extracellular matrix does not have an obvious nuclear structure, and DNA (Deoxyribonucleic Acid) determination shows that the concentration of an acellular group is obviously reduced (P is less than 0.01); along with the increase of the dECM concentration, the porosity is reduced, and the degradation time is prolonged; the airway stent is printed in a 3D biological printing mode, no obvious cytotoxicity (P is larger than 0.05) exists in a cytotoxicity experiment, and the mechanical property part of the airway stent is similar to that of a silicone stent.

The invention relates to the technical field of electronics, in particular to a scanning equipment control process management method based on a custom protocol. According to the method provided by the invention, a secure communication bridge between a Web environment and local hardware is established through interaction between a custom protocol and an operating system registry; a driver and an API interface matched with a configuration file are dynamically loaded by using a local plug-in, so that multi-model scanning equipment is compatible, and the hardware adaptation and maintenance complexity is reduced; original data is converted into standardized data, an atomized transactional data processing pipeline is constructed, and the integrity and traceability of cryopreservation tube information are ensured; finally, a visual interface is dynamically analyzed and rendered based on a Web client, real-time mapping of cryopreservation tube layout is achieved, and operation intuition, sample searching efficiency and user experience are improved.

The present invention provides preparations of MSCs with important therapeutic potential. The MSC cells are non-primary cells with an antigen profile comprising less than about 1.25% CD45+ cells (or less than about 0.75% CD45+), at least about 95% CD105+ cells, and at least about 95% CD166+ cells. Optionally, MSCs of the present preparations are isogenic and can be expanded ex vivo and cryo-preserved and thawed, yet maintain a stable and uniform phenotype. Methods are taught here of expanding these MSCs to produce a clinical scale therapeutic preparations and medical uses thereof.

Methods, systems, and compositions are provided for extracting bone marrow cells from bone obtained from deceased donors, for preparing the bone marrow for cryopreservation, and for obtaining desired cells from cryopreserved and fresh bone marrow

Some embodiments relate to improved cryopreservative formulations and improved methods for cryopreserving and thawing cells. These formulations facilitate high density, low volume cell cryopreservation strategies which overcome the need for washing of cells post-thaw, and therefore result in greater cell recovery and viability. Pharmaceutical compositions comprising a cell population and a cryopreservative formulation as provided herein are also provided. Methods for treating a subject having a disease or condition that benefits from transplantation of a cryopreserved and thawed cell population are also provided.

The utility model discloses a kind of multiple non-matrix automatic cap screwing workstations for cryopreservation tube, including bottom installation component, support component is provided on bottom installation component, support component top end is provided with top installation component, top installation component is provided with outer transmission component, first moving nut, second moving nut are provided on outer transmission component, first moving nut is provided with first moving plate, first moving plate is provided with a plurality of first installation hole, inner screwing transmission component is provided in first installation hole, second moving nut is provided with second moving plate, second moving plate is provided with a plurality of second installation hole, moving guide rail is provided on bottom installation component, feeding moving plate is provided on moving guide rail, feeding moving plate is provided with cryopreservation tube storage component, use this automatic cap screwing workstation, realize the automatic screwing cover and unscrewing cover operation to batch cryopreservation tube, effectively improve the operation efficiency that cover is screwed or unscrewed.

The invention relates to the field of cells, in particular to a cell culture method which comprises the following steps: S1, taking out a cryopreservation tube from liquid nitrogen; s2, quickly putting the mixture into a water bath, and shaking the mixture from time to time to quickly melt the mixture; s3, wiping and disinfecting a cryopreservation tube with alcohol, opening a cover, injecting the cell suspension into a centrifugal tube by using a suction tube, dropwise adding a culture solution, and carrying out low-speed centrifugation; s4, pouring out the supernate, and adding a culture solution to suspend the cells; s5, sucking the cells into a culture dish filled with a culture solution, and slightly shaking all around, so that the cells in the culture dish are uniformly distributed; s6, placing the culture dish in an incubator at 37 DEG C for culturing; and S7, during culture, replacing a culture solution in the culture dish through a solution replacement mechanism arranged on the culture box in a closed state of the culture box. According to the invention, the culture solution of the culture dish can be replaced when the culture box is closed.

The utility model discloses a stem cell storage tank convenient to take and place in the technical field of stem cell storage, which comprises a storage tank body used for storing stem cells and a placing frame used for placing the stem cells, the storage tank body comprises a belly part used for storing liquid nitrogen and a neck part used for preventing the liquid nitrogen from overflowing, a sealing cover is arranged on the neck part, and the storage tank body is provided with an opening. A supporting frame is arranged on the upper middle portion of the neck part, a plurality of openings separated through partition plates are formed in the supporting frame, two first shafts are arranged at the tops of the openings, first doors are symmetrically arranged on the two first shafts respectively, two second shafts are arranged at the bottoms of the openings, and the two second shafts are connected with the partition plates. The stem cell cryopreservation device solves the problems that existing stem cells are stored in a liquid nitrogen storage tank through a cryopreservation tube, however, the liquid nitrogen tank needs to be opened in the storing and taking process, part of liquid nitrogen escapes from the storage tank due to convection between the liquid nitrogen and the atmosphere in the opening process, and when the escaped liquid nitrogen is too much, the stem cells cannot be stored in the liquid nitrogen storage tank. And workers are easily injured.

The utility model relates to the technical field of cell cryopreservation, in particular to a cell cryopreservation box matched with straw, which comprises a cell cryopreservation box, the cell cryopreservation box comprises a bottom box and a box cover, the bottom box and the box cover are arranged in a rectangular structure, and the box cover is matched and buckled on the bottom box. A plurality of spacing columns used for spacing straws are uniformly arranged in the bottom box, a plurality of liquid nitrogen inlet grooves used for liquid nitrogen to enter are formed in the middle of the interior of the bottom box in a penetrating mode, protruding blocks are arranged on the outer walls, close to the top corners of the periphery, of the bottom box, the protruding blocks are of an L-shaped structure, and when the box cover is buckled on the bottom box, the bottom of the box cover abuts against the tops of the protruding blocks; compared with the prior art, the cell cryopreservation box can store a large number of straws, the sealed straws can be placed in the cell cryopreservation box, the operation process is simplified, additional materials such as circular thumb tubes, low-temperature storage aluminum strips and special liquid nitrogen tanks are not needed, the operation difficulty and cost are reduced, and the purposes that the straws are convenient to place, observe and operate are achieved.

The invention discloses a preparation method of metal organic framework armored red blood cells and an application of the red blood cells in cryopreservation, an MOF armor coated on the surfaces of the red blood cells in situ in one step is prepared in a physiological environment, the red blood cells can be effectively prevented from being damaged by freezing induction, and meanwhile, the natural oxygen carrying function of the red blood cells is kept. The unique ice control capacity of the MOF armored red blood cells is explored, including inhibition of recrystallization of ice, induction of smoothness of the ice front, reduction of formation of sodium chloride eutectic and acceleration of ice melting, a novel design strategy is provided for an MOF-based ice control material, and the future prospect of the MOF-based ice control material in the field of efficient ice inhibition is possibly widened.

The present invention provides means with which bile acids, phenols, indoles and organic acids contained in a sample can be stably preserved without cryopreservation of the sample. A sample preservation solution of the present invention is a sample preservation solution used for analyzing at least one component selected from the group consisting of bile acids, phenols, indoles and organic acids contained in a biological or environmental sample, containing at least the following (A) and (B): (A) condensed phosphate or polyoxyethylene sorbitan alkylate; and (B) guanidinium thiocyanate, Tris-HCl (pH 7 to 9), and EDTA.

1. The name of the design product: cryopreservation rack. 2. The use of the design product: the design product is used for the cryopreservation rack of the specimen in the ultra-low temperature refrigerator. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The utility model belongs to the field of cryopreservation tubes, and discloses a biological sample cryopreservation tube with an RFID (Radio Frequency Identification Device) electronic label, which comprises a tube body, the tube body is of a hollow structure with two open ends, and an interlayer is fixedly connected inside the tube body and divides the internal space of the tube body into two drawing cavities; the drawer box is detachably inserted into the drawing cavity, one end of the drawer box is fixedly connected with a connecting sleeve, a sliding groove is formed in the side wall of the connecting sleeve, a pushing rod is slidably inserted into the connecting sleeve, the inner side end of the pushing rod is fixedly connected with a connecting rod, and the other end of the connecting rod penetrates through the sliding groove to be fixedly connected with the sliding rod; the outer side end of the push rod is fixedly connected with a pressing plate, and the peripheral side of the push rod is sleeved with a spring. And the tube cover is rotationally connected to the connecting sleeve, and the tube cover is detachably connected with the end part of the tube body. The internal space of the tube body is divided into the two drawing cavities by the interlayer, the drawer box can be mounted in each drawing cavity, and the tube cover is connected with the end part of the tube body, so that the utilization rate of the internal space of the tube body is greatly improved.

The utility model discloses a multi-stage dehydration device for stem cell vitrification cryopreservation, which comprises a base, the top of the base is provided with a containing groove, the containing groove is internally provided with a storage pipe for storing stem cell suspension and a [-shaped frame, the top of the [-shaped frame is provided with a lifting assembly, and the lifting assembly is provided with a water inlet and a water outlet. The movable end of the lifting assembly is fixedly provided with an injection pipe, the outer wall of the injection pipe is fixedly communicated with a peristaltic pump through a pipeline, through linkage control of the lifting assembly and the peristaltic pump, automatic conveying of a dehydrating agent and accurate lifting of the injection pipe are achieved, manual pipetting or container replacement is not needed, the operation efficiency is remarkably improved, and the labor intensity of workers is reduced. The quick plugging design of the quick-plugging male connector and the quick-plugging female connector supports switching of multiple groups of dehydrating agent storage tanks as required, ensures continuous gradient progression of dehydrating agents with different concentrations, realizes gradual dehydration of stem cell suspension in a gradient concentration environment, realizes smooth transition of the concentration of the dehydrating agent, and is beneficial to further improvement of the survival rate of stem cells.

This invention provides a dry ice transport insulated box for cell products, belonging to the technical field of transport boxes. It includes a box body and lid secured by latches. Inside the box, partitions separate storage compartments for cryopreservation bags, cryopreservation tubes, and dry ice. The dry ice storage compartments are located around the cryopreservation bag and cryopreservation tube storage compartments. Two thermometers are fixed to the side of the box body, with their probes extending into the cryopreservation bag and cryopreservation tube storage compartments respectively, allowing real-time monitoring of the box's internal temperature. The cryopreservation bag storage compartment is used to store strip-shaped cryopreservation boxes containing cryopreservation bags, while the cryopreservation tube storage compartment is used to store square cryopreservation boxes containing cryopreservation tubes. This invention isolates the storage compartments for each cell, preventing collisions and impacts on cell quality stability during transport.

Methods for cryopreservation of biological samples are provided. The biological samples are sub-millimeter or millimeter scale biological materials. The cryopreservation system includes the use of a conduction-dominated cryomesh (CondD-C). The methods include loading the CondD-C with biological samples and submerging the CondD-C with the samples in a manner, e.g., by vertical plunging, that removes the vapor bubbles from the cooling surfaces. Methods using the CondD-C and vertical plunging can enhance the cooling rate and warming rates and are scalable for high throughput processing of large numbers of vitrified and / or rewarmed biological samples.

The invention provides a biological cell freezing visualization system and method based on a metal woven belt, and relates to the technical field of biologication.The biological cell freezing visualization system comprises a bottom plate, a support platform, a profound hypothermia liquid tank and an observation assembly are fixedly connected to the upper surface of the bottom plate, a lifting sliding table is fixedly connected to the inner bottom face of the support platform, and a constant hypothermia copper table is fixedly connected to the upper end of the lifting sliding table; the constant hypothermia copper table is communicated with the deep hypothermia liquid tank through a liquid conveying hose, the inner top surface of the support platform is fixedly connected with a woven belt fixing support and a sample placing platform, one end of a metal woven belt is fixedly connected to the lower surface of the woven belt fixing support, and the other end of the metal woven belt is fixedly connected to the lower surface of the sample placing platform; a cover plate covers the sample placing platform, and the observation assembly is used for shooting an image of the sample placing platform. Cold is conducted through the metal woven belt, the requirement for the movement precision of the device is low, the durability of the device is improved, the position of the sample containing platform is fixed, and it is guaranteed that imaging of the observation assembly is clear.

The invention provides an automatic urine sample preprocessing method and system for pharmacokinetic parameter calculation. The method is realized through an integrated automatic process as follows: identifying a urine cup with an identity label, transferring a urine sample to a measuring cylinder, accurately measuring the volume of urine by using a sensor, adding a stabilizing solution according to a preset proportion, then performing standardized mixing to form a mixed solution, and finally sub-packaging the mixed solution into a cryopreservation tube in a high-flux manner. The technical problem that in the prior art, due to manual operation, urine volume measurement is not accurate, medicine components are degraded after being in vitro, data tracing is difficult and the like is solved, and finally the technical pain point that key pharmacokinetic parameter calculation results are seriously inaccurate is caused. By providing a high-precision and high-reproducibility qualified sample, the quality of subsequent analysis data is guaranteed from the source, and the accuracy, research efficiency and data compliance of PK parameter calculation are remarkably improved.

The utility model discloses a cryopreservation tube sorting device, and belongs to the technical field of biological experiment equipment. A cryogenic vial sorting device comprises a bottom frame, a sorting box is installed on the bottom frame, a plurality of sorting holes for sorting cryogenic vials are formed in the bottom wall of the sorting box, a push plate is arranged between the bottom frame and the sorting box, sliding grooves are formed in the two sides of the bottom frame respectively, and the push plate is arranged in the sliding grooves. Thrust seats located in the pair of sliding grooves respectively are installed at the two ends of the push plate, and locking pieces are arranged between the fixing seat and the thrust seats. According to the cryopreservation tube sorting device, by means of the ingeniously-designed sorting holes and push plate structures, a user can easily and accurately sort cryopreservation tubes, the special design of the sorting holes ensures that only the cryopreservation tubes meeting specific conditions (for example, the cryopreservation tubes are upright but not inverted) can pass through, the sorting accuracy is greatly improved, and meanwhile the sorting efficiency is greatly improved. And due to the design of the push plate, the cryopreservation tubes can be quickly pushed upwards after sorting is completed, the sorting process is simplified, and the overall efficiency is improved.

The utility model provides a cell cryopreservation bag. The cell cryopreservation bag comprises a bag body, a sample introduction part and a sampling part, the bag body comprises a plurality of communicated cavities, and a channel is formed between every two adjacent cavities so that the adjacent cavities can be communicated. The sample introduction part comprises a sample introduction port and an air sample introduction pipe connected with the sample introduction port, and the air sample introduction pipe is used for being connected with the external atmosphere so that the air pressure in each cavity can be kept consistent with the external atmospheric pressure. The sampling part is arranged on the bag body and corresponds to the cavity. According to the cell cryopreservation bag, the air sample injection pipe is arranged on the sample injection part, and the channels are formed between the cavities, so that the cavities can be communicated with the outside atmosphere after sample injection is completed, cell suspension samples can be automatically and uniformly subpackaged into the cavities, the subpackaging uniformity of the cell suspension samples is improved, and the subpackaging efficiency of the cell suspension samples is improved. Manual extrusion of an operator is not needed, so that the operation is convenient, the time is shortened, and the cell cryopreservation efficiency is improved.

The invention relates to the technical field of sheep semen storage, and discloses a sheep fresh semen low-temperature long-acting preservation diluent containing indole-3-pyruvic acid, the sheep fresh semen low-temperature long-acting preservation diluent containing indole-3-pyruvic acid comprises a basic diluent and an additive, the additive is indole-3-pyruvic acid, and the concentration of the additive in the diluent is 15 mg / L-35 mg / L. The basic diluent comprises the following components: 3.1 g of glucose; 1.4 g of sodium citrate; 20 mL of yolk; 100 [mu] L of lincomycin hydrochloride; 0.50 g of bovine serum albumin; and 0.50 g of vitamin C. The preparation method of the basic diluent comprises the following steps: dissolving glucose, sodium citrate, bovine serum albumin and vitamin C in distilled water, oscillating, filtering and sterilizing, adding yolk and lincomycin hydrochloride, uniformly mixing to obtain the basic diluent, and sealing and storing at 4 DEG C. According to the sheep fresh sperm low-temperature preservation diluent containing indole-3-pyruvic acid provided by the invention, 25 mg / L of indole-3-pyruvic acid is added into the diluent, so that the preservation quality and time efficiency of sheep sperms under the low-temperature condition of 4 DEG C can be improved.

The embodiment of the present application provides a turned-over liquid nitrogen tank cover and liquid nitrogen tank, and relates to the technical field of cryopreservation equipment. It aims to improve the problem that the liquid nitrogen tank cover is inconvenient to open and close and inconvenient to observe. It comprises an outer cover, an inner cover and a sensor. The outer cover comprises a cover body, an exhaust fan and a pressing plate. A communication hole is arranged between the inner wall and the outer wall of the cover body, and an exhaust passage is communicated with the outside. The pressing plate is used to move downward under the action of external pressure to make the exhaust passage conductive, or the pressing plate is used to move upward after reset to block the exhaust passage. The inner cover is rotatably arranged in the middle of the cover body. The inner cover is used to close or open the hole in the process of rotation, and to separate or press the pressing plate. The opening and closing of the hole in the cover body are controlled by the sensor. The rotation of the inner cover realizes the conduction or blocking of the exhaust passage, so as to realize the removal of white mist and the closing of the hole. The degree of automation and efficiency are high, and the use efficiency of the liquid nitrogen tank can be improved.

The invention discloses a vitrification protective agent for retina organoid and application of the vitrification protective agent, and belongs to the technical field of organoid cryopreservation. The vitrification protective agent provided by the invention consists of 22wt% of dimethyl sulfoxide, 8wt% of ethylene glycol, 0.5 M sucrose and the balance of a culture medium. The invention also provides an application of the vitrification protective agent in retina organ cryopreservation. According to the invention, by improving the components of the protective agent, optimizing the loading and removing mode of the protective agent and selecting the cryopreservation carrier, the high-quality preservation of the retinal organs is realized. Compared with the prior art, the method has the advantages that the vitrification concentration is low, the retina organoid is fast to recover, the structural function is completely preserved, freezing and resuscitation of the retina organoid can be efficiently achieved in a high-throughput and simple-operation mode, and application requirements can be met.

The invention relates to the technical field of storage devices, in particular to a three-dimensional automatic cryopreservation box storage and transfer system which comprises a vertical cabinet and a conveying rail arranged in the vertical cabinet, a cryopreservation box is clamped to the top of the conveying rail, a propelling part is arranged in the cabinet, a transfer plate is arranged on the outer side of the propelling part, and a driving part is arranged at the bottom of the transfer plate. The outer side of the driving part is in transmission connection with a connecting piece and a movable plate, a suction system is fixed to the top of the transfer plate, a threaded rod is rotationally connected to the inner side of the cryopreservation box, a positioning plate is connected to the outer side of the threaded rod, an auxiliary piece is rotationally connected to the inner side of the positioning plate, and a valve piece is fixed to the bottom of the positioning plate. The problems of pollution and frosting caused by manual operation or mechanical arm operation are solved.

The utility model relates to the technical field of medical experiment tools, in particular to an automatic cover opening tool which comprises a lower shell, an upper shell, a battery, a mounting plate, a motor, a connecting rotating shaft, a profiling head, a control circuit board, a cover pushing mechanism, an electric quantity indicator lamp, a charging port, a starting switch, a cover opening button and a cover screwing button. By means of the structural design that the profiling heads are detachable and replaceable, automatic uncapping and uncapping of freezing tubes of various specifications can be achieved by replacing different profiling heads, good compatibility is achieved, product assembling consistency is kept, automatic uncapping and uncapping of a plurality of bottles can be achieved at a time through the arrangement of the multiple profiling heads, and the production efficiency is improved. Meanwhile, manual contact with the cryopreservation tube is avoided, cleanliness and no pollution of the environment in the uncovering process are guaranteed, manual work is reduced, the uncovering efficiency is improved, the experiment efficiency is improved, and good practicability is achieved.

The utility model relates to the technical field of sample storage, and provides a sample storage device which comprises a storage shelf, a cryopreservation box bracket and a manipulator, and an access opening is formed in one side of the storage shelf; the multiple cryopreservation box brackets are arranged in the storage shelf at intervals in the height direction of the storage shelf, and dragging grooves are formed in the sides, close to the storing and taking openings, of the cryopreservation box brackets; the mechanical arm comprises a towing hook capable of moving on the side, close to the storing and taking opening, of the storage goods shelf, and the towing hook is used for being embedded into the dragging groove so as to push and pull the corresponding cryopreservation box bracket. The storage density of the sample storage device can be improved, and meanwhile, the convenience of sample access is kept.

The invention belongs to the technical field of biology, and particularly discloses a chicken semen cryopreservation solution and a preparation method thereof. The chicken semen cryopreservation liquid comprises a cryoprotection liquid I and a cryoprotection liquid II. The freezing protection liquid I is prepared from the following components: glucose, sodium glutamate, potassium acetate, dipotassium phosphate, magnesium acetate, N, N-bis (2-ethoxyl)-2-aminoethanesulfonic acid and an antibiotic solution. And the freezing protection liquid II comprises dimethylformamide and nano zinc oxide. The invention discloses a chicken semen cryopreservation solution and a preparation method thereof, by applying the chicken semen cryopreservation solution, the fertilization and incubation performance of frozen semen is remarkably improved, centrifugation-free and direct semen deposition of the frozen semen are realized, and physical damage and activity reduction caused by the centrifugation process to sperms are avoided.

The utility model discloses a low-temperature preservation device for mixed solution, which comprises a device body and a box door, a square plate is embedded in the device body, a frame body is arranged on the surface of the square plate, a filter screen is embedded on the surface of the frame body, absorbent cotton is embedded in the frame body, and a fixing block on the surface of the frame body is connected with a fixing rod. The fixing rods are symmetrically distributed, and buckles are embedded in the device body. According to the low-temperature storage device for the mixed solution, a square plate is embedded in the device body, before the solution needs to be placed, a frame body is firstly installed on the surface of the square plate, the frame body is installed on the square plate in a sliding mode, at the moment, a fixing rod is clamped into a buckle, and then the frame body can be fixed; at the moment, the container filled with the mixed solution can be easily placed on the filter screen on the surface of the frame body, and the fused solution attached to the outer wall of the container can directly drop on the absorbent cotton through the filter screen, so that the liquid is effectively prevented from flowing around, and the container is relatively convenient to use.