1054 results about "Cryopreservation" patented technology

The invention discloses a biological sample low-temperature preservation box, and particularly relates to the technical field of DNA sample preservation, the biological sample low-temperature preservation box comprises a box body and a first box door, the first box door is movably connected with the box body through a hinge, a touch screen, a first camera and a fingerprint lock are fixedly installed at the top end of the box body, and a second camera is fixedly installed on the front side of the box body; a semiconductor refrigerator is fixedly installed in the box body, a second box door is movably arranged on the rear side of the first box door, and the second box door is movably connected with the front side of the box body through a hinge. The threaded ice storage barrel is rotated to be far away from the box body, then ice blocks are placed in the threaded ice storage barrel, finally the threaded ice storage barrel is installed and fixed, auxiliary refrigeration is conducted on the interior of the box body, and meanwhile, along with gradual melting of the ice blocks, generated water enters the water storage barrel through the guide pipe; the one-way valve can prevent water in the water storage barrel from flowing back into the threaded ice storage barrel, the structure is simple, and auxiliary refrigeration can be conveniently carried out on the interior of the box body.

The utility model relates to the technical field of picking and moving of biological sample cryopreservation tubes, and discloses a cryopreservation tube anti-falling structure which comprises a tube picking assembly, an anti-falling movement assembly, a fixing assembly and a control module, and the tube picking assembly and the anti-falling movement assembly are both installed on the fixing assembly. The pipe picking assembly is composed of a cryopreservation pipe, a suction head, a suction head spring, a linear bearing and an air pipe connector. The cryopreservation tube picking assembly is arranged, the cryopreservation tube is sucked through negative pressure, the cryopreservation tube is blown away through positive pressure, the cryopreservation tube picking device is simple in structure, few in component parts, small in size, higher in adaptability, low in manufacturing cost and stable and reliable in operation, the anti-falling movement assembly is arranged, two clamping jaws are pressed through a hinge pin spring to clamp the cryopreservation tube, and the cryopreservation tube is prevented from falling accidentally; the cryopreservation tube clamping device is simple in structure, low in manufacturing cost and stable and reliable in operation, the cryopreservation tube can be clamped to be prevented from falling off even if power failure or air leakage occurs due to the fact that the spring is used for pressing the two clamping jaws to clamp the cryopreservation tube, and maintenance is convenient.

The invention provides a preparation method of Al18F tracer integrated freeze-dried powder, a kit and application thereof, and the preparation method comprises the following steps: dissolving 1-10 mg of a precursor by using deionized water, adding a pH buffer solution with the concentration of 0.2-0.5 M and an AlCl3 solution with the concentration of 10 mM, with the ratio of the amount of substance of AlCl3 to the amount of substance of the precursor being 0.8-1.0, then continuously adding a stabilizer solution with the concentration of 0.5 M and 1-4 mg of an excipient, and uniformly stirring to obtain the Al18F tracer integrated freeze-dried powder. And finally, adding deionized water to enable the concentration of the precursor to be 0.5-1.0 mM, uniformly mixing the solution, sub-packaging the solution into 1.5 mL cryopreservation tubes or 2 mL penicillin bottles according to a certain volume, and then putting the cryopreservation tubes or the penicillin bottles into a vacuum freeze dryer to remove water at a low temperature to obtain powder or freeze-dried cakes, and the container is a freeze-dried powder / cake storage container and is used for storing the freeze-dried powder / cakes. Meanwhile, the reaction container is also used during radioactive labeling.

The utility model discloses a biological sample low-temperature preservation device. A freezing storage set and a rotating assembly are placed in a low-temperature storage cavity; the bottom of the lifting part is arranged at the top, located in the transition area, of the heat preservation tank body, a lifting assembly is arranged at the top of the lifting part, and a lifting part of the lifting assembly can sequentially penetrate through the heat preservation taking and placing part and the transition part and extend into the heat preservation part; the first liquid level sensor, the liquid inlet pipeline, the first temperature sensor and the second liquid level sensor are all located in the heat preservation tank body, the first liquid level sensor and the liquid inlet pipeline are both close to the lower surface of the rotating disc, the first temperature sensor is located on the peripheral wall face of the rotating disc, and the second liquid level sensor is located on the inner top of the heat preservation tank body. The liquid inlet valve is communicated with the liquid supplementing valve, the liquid supplementing valve is communicated with the liquid inlet pipeline, the exhaust valve is communicated between the liquid supplementing valve and the liquid inlet valve, and the exhaust valve is communicated with the vertical channel; the second temperature sensor is located in the cylinder. When the cryopreservation box is taken and placed, the cryopreservation box is in a low-temperature area of-80 DEG C to-170 DEG C, so that the biological sample is always in a protection state.

The invention discloses a ready-to-use activated human umbilical cord mesenchymal stem cell as well as a preparation method and application thereof. The preparation method comprises the following steps: carrying out passage on P0-generation cells for three times to obtain P3-generation cells; cryopreserving P3 generation cells, and establishing a cell seed bank; resuscitating the cells in the seed bank for three times of passage to obtain P6-generation cells; and carrying out activation culture on the P6-generation cells, and resuspending the activated P6-generation cells in a cell protective agent to obtain the human umbilical cord mesenchymal stem cell original suspension. The invention also discloses an umbilical cord mesenchymal stem cell cryopreservation preparation. The cryopreservation preparation is obtained by mixing the human umbilical cord mesenchymal stem cell original suspension with a cryopreservation protective agent, sub-packaging and cryopreserving. Experiments prove that the human umbilical cord mesenchymal stem cell cryopreserved preparation prepared by the invention can be stored for a long time, is convenient to store, transport and use clinically, can complete effectiveness and safety index detection within the shelf life, and has the same activity as a fresh preparation.

The invention provides a preparation method of a skull for autologous replantation, and relates to the technical field of skull treatment. The method comprises the following steps that the taken-out autologous skull is subjected to ultraviolet radiation and then conveyed into a clean room to be subjected to photographing treatment; sequentially carrying out iodophor soaking, alcohol soaking and normal saline flushing on the skull, and then carrying out surface tissue impurity removal and cleaning on the skull; putting the treated skull into a packaging bag, adding a cryopreservation solution to wrap the skull, and then performing vacuumizing and sealing treatment; and sterilizing and cooling the skull subjected to the sealing treatment, and finally feeding the skull into a bone library for cryopreservation. According to the method, the skull of a patient is taken out after an operation to serve as an autologous bone raw material, so that the skull has bone activity within a certain period of time, finally, the skull is replanted to the skull of the patient, the body burden and conflict psychology of the patient are reduced, the exclusive transportation, treatment and storage modes are adopted, the rejection of the patient is reduced, the risk of bone resorption is reduced, and the bone activity is guaranteed; the replanting rate is increased.

The utility model relates to the technical field of sample preservation, and discloses a quick storing and taking device for a liquid nitrogen tank cryopreservation box, which comprises a machine body, support frames are fixedly connected to the periphery of the outer surface of the machine body, a mounting frame is fixedly connected to one side in the machine body, and a movable door motor is fixedly mounted on one side of the mounting frame through bolts. According to the utility model, the whole equipment is arranged, the access of the cryopreservation box is controlled through the moving door motor, the operation can be quickly and accurately completed, the device is particularly suitable for processing a large number of samples, the working efficiency is obviously improved, the labor intensity is reduced through the electric operation mode, the physical output of personnel when the samples are frequently accessed is reduced, and the labor intensity is reduced. Through cooperation of the jacking motor and the moving door lead screw, the cryopreservation box can be adjusted in a self-adaptive mode, it is ensured that the cryopreservation box is safely and stably stored, the cryopreservation boxes of different layers can be conveniently stored and taken out, the cryopreservation box can be rapidly moved into or out of the liquid nitrogen tank through combination of the conveying motor, the transmission shaft and the conveying belt, and the working efficiency is improved.

The invention belongs to the technical field of biological culture, and particularly relates to a chlorella extracellular fluid and a preparation method and application thereof.The preparation method of the chlorella extracellular fluid comprises the following steps that a BG11 culture medium is inoculated with a chlorella solution cultured to the logarithmic phase, oxcarbazepine is added, and the chlorella extracellular fluid is obtained; the preparation method comprises the following steps: placing chlorella in an intermittent illumination environment with the temperature of 20-30 DEG C and the rotating speed of 100-200 rpm for growth, centrifuging the chlorella to obtain supernate after the chlorella grows to the 10th-16th day, filtering, sub-packaging, and performing cryopreservation to obtain the chlorella extracellular fluid. By adding oxcarbazepine, the chlorella extracellular fluid prepared in a specific culture medium and a specific growth environment has high oxidation activity, excellent stability and excellent degradation capacity, and a new thought is provided for solving the pollution treatment problem of current environmental pollutants such as pharmaceutical products, polycyclic aromatic hydrocarbons and dyes.

The present invention provides an extracellular vesicle protectant and its application. The extracellular vesicle protectant has a simple composition, including a buffer system, a saccharide substance, and a surfactant. It can be used as a cryopreservation solution or a lyophilization protectant, and has a wide applicable temperature range. The protectant can reduce the storage and transportation cost of exosomes and expand the applicable range of exosomes. The exosome lyophilized powder prepared by using the above protectant and exosomes can be stably stored for a long time. The lyophilized powder can be realized on a large-scale lyophilization production device, making it possible to industrially produce exosomes on a large scale.

The utility model relates to the field of experimental consumables, in particular to a cryopreservation tube protective cover and a cryopreservation tube. The cryopreservation tube protection cover is matched with a tube body and a cover body of a cryopreservation tube and comprises a shell and a separation membrane. The housing has a first section and a second section opposite the first section. The first section of the shell is matched with the pipe body and connected with the pipe body in a sealed mode. And the second section of the shell is matched with the cover body and is detachably connected with the cover body. The edge of the separation film is connected to the inner wall of the shell and used for closing the opening of the pipe body. The cryopreservation tube comprises the tube body, a cover body and a protective cover. Whether the cryopreservation tube is opened for the first time or not is judged by judging whether the separation membrane is punctured and damaged or not, and the uniqueness function of a biological sample can be achieved easily. A sample is protected by the separation membrane after the cover body is opened and before sampling, so that the cleanliness of the sample is improved.

The invention discloses a method for establishing a cell line by serum-free culture of PGCs (Primordial Germ Cells) of breeding poultry, the cell line is prepared by blending calcium-free DMEM (Dulbecco Modified Eagle Medium), water and Knockout-DMEM, the cell line is more suitable for osmotic pressure and calcium ion concentration required by proliferation of the PGCs of the breeding poultry, various components of serum-free culture are perfected by using fresh egg white, and the cell line is more suitable for proliferation of the PGCs of the breeding poultry. The combination of growth factors required by primordial germ cells is optimized by adding the FGF-1, so that 100% of cell line establishment efficiency and rapid growth of cells from reproduction crescent, blood and gonad tissues of chicken embryos are realized, and a technical basis is provided for poultry frozen breed conservation, stem cell propagation and gene editing operation.

The invention discloses a method for extracting mesenchymal stem cells from urine. The method comprises the following steps: (1) sampling and inoculating urine; (2) amplification and passage of mesenchymal stem cells; (3) identifying the urine stem cells; and (4) a cryopreservation and resuscitation method of the urine stem cells. And performing biological phenotype identification according to the standard formulated by the International Cell Therapy Association to confirm the mesenchymal stem cells. The method is low in cost, simple and rapid, and the used culture solutions are all commercialized and are very convenient to purchase and use. The culture solution special for the stem cells is used in the first two weeks of the extraction period, after passage, when enough stem cells are obtained, the culture medium can be replaced with a common DMEM culture medium, and the manufacturing cost is greatly reduced. And secondly, the materials are simple, rapid and non-invasive, and the sample of the method for extracting the mesenchymal stem cells from urine comes from a patient, so that the problem of heterogeneity of cell sources is reduced. And a foundation is laid for stem cell treatment and gene editing treatment of genetic diseases and major difficult diseases.

The invention discloses a construction method of a human epidermal organoid, and belongs to the technical field of organoid cell culture. The method is mainly used for optimizing a culture method of human epidermal organoid, and particularly relates to an efficient epidermal organoid culture technology based on a specific culture medium and an enzyme digestion system. According to the method, a # imgabs0 # human epidermal organ complete culture medium is adopted for preparation, and a basic culture medium and an additive are mixed to form the complete culture medium suitable for cell growth. The primary specimens are treated, and the steps of cleaning, digestion, cell collection and the like are included, so that the activity and functions of cells are ensured. Through accurate inoculation and culture conditions, the formation and growth of the organoid are promoted, and the culture in a proper environment is ensured, so that the formation efficiency of the organoid is improved. The organoid is subjected to passage and cryopreservation treatment, so that the activity and functional stability of the organoid in the long-term storage and use process are ensured.

The invention discloses hydrogel bio-ink, a preparation method of the hydrogel bio-ink, a 3D printing biodegradable airway stent and application of the 3D printing biodegradable airway stent. The preparation method comprises the following steps that S1, lung tissue is subjected to freeze thawing, slicing, sequential decellularization of SDS and TritonX-100, air drying, smashing and cryopreservation, and a lung extracellular matrix is obtained; s2, performing sterilization and pepsin treatment on the lung extracellular matrix prepared in S1 to obtain a pretreated lung extracellular matrix; and S3, mixing methyl propionylated gelatin and an alginate solution, pouring the mixture into solid gel, gelatinizing the solid gel, and adding the pretreated lung extracellular matrix to prepare the hydrogel bio-ink. The prepared pig lung acellular extracellular matrix does not have an obvious nuclear structure, and DNA (Deoxyribonucleic Acid) determination shows that the concentration of an acellular group is obviously reduced (P is less than 0.01); along with the increase of the dECM concentration, the porosity is reduced, and the degradation time is prolonged; the airway stent is printed in a 3D biological printing mode, no obvious cytotoxicity (P is larger than 0.05) exists in a cytotoxicity experiment, and the mechanical property part of the airway stent is similar to that of a silicone stent.

The present invention discloses an application of highly active human umbilical cord mesenchymal stem cells. HA-MSCs were isolated from human umbilical cord tissue using TeSR-E8 containing 5% CloneR and LN521-Coated culture flasks, and a technical system for HA-MSC isolation, expansion, and cryopreservation was established. Compared with conventionally cultured MSCs, the HA-MSCs prepared by the present invention have higher proliferation activity, smaller size, a higher nuclear-cytoplasmic ratio, and highly express embryonic stem cell-associated marker antigens SOX2, Nanog, and OCT4. They have the potential to differentiate into neural, myocardial, and hepato-intestinal progenitor cells derived from the three germ layers and possess excellent anti-aging activity.

The invention discloses a culture medium composition for culturing GDT cells and a culture method, and belongs to the field of biological medicine. Aiming at the problems of low in-vitro amplification efficiency and poor function of the existing GDT cells, the invention provides the culture medium composition for culturing the GDT cells, the culture medium composition comprises an induction culture medium and a proliferation culture medium, the induction culture medium comprises a basic culture medium and an activating agent, and the activating agent comprises IL-7 and paclitaxel; the proliferation culture medium comprises a basic culture medium, IL-18 and a metabolism regulator. An STAT5 pathway is activated through IL-7, and amplification of the Vdelta2 subgroup is specifically promoted; on the other hand, through the cooperation of the IL-7 and the IL-18, the IL-18 activates the expression of the NKG2D, and the IL-18 and the IL-7 jointly enhance the cytotoxicity; efficient amplification and function enhancement of the GDT cells are achieved; the killing rate of PD-L1 + tumor cells is larger than 80%, the motility rate after cryopreservation recovery is larger than 90%, the function maintenance rate is larger than 85%, and the cell line is suitable for tumor immunotherapy, infectious disease treatment and cell drug development.

The present disclosure provides, among other things, a method for cryopreserving Natural Killer cells (NK cells) comprising washing cultured NK cells in a medium comprising Minimal Essential Medium and resuspending in a cryoformulation medium comprising MEM, albumin and one or more cryoprotective agent, and compositions thereof. In some aspects, provided herein is a method of culturing natural killer (NK) cells in a culture medium comprising an AMPK activator, nicotinamide, arginine supplement, optionally comprising a lipid supplement, and / or cultured in between 1-21% oxygen.

The invention discloses an anti-freezing solution for improving the survival rate of sperms of Chinese prawns and a cryopreservation method of the anti-freezing solution, and belongs to the technical field of low-temperature biology and reproductive science. The invention provides an anti-freezing solution for cryopreservation of sperms of Chinese prawns, which comprises a basic solution and an anti-freezing agent, and the anti-freezing agent is dimethyl sulfoxide with the volume fraction of 10% or glycerol with the volume fraction of 10%; the basic liquid contains 23.69 g of sodium chloride, 1.1 g of potassium chloride, 14.8 g of magnesium chloride hexahydrate, 4.3 g of sodium sulfate, 0.23 g of sodium bicarbonate, 1.12 g of calcium chloride and distilled water with the constant volume of 1000 mL. According to the cryopreservation method provided by the invention, 10% dimethyl sulfoxide or 10% glycerol is adopted as an antifreeze agent, the sperm density is controlled to be 1 * 10 < 7 > / mL, and step-by-step cooling is carried out, so that the survival rate of unfrozen sperms reaches 90% or above. The long-term preservation effect of the Chinese prawn sperms is remarkably improved, and the method is suitable for germplasm resource protection and efficient breeding.

The invention belongs to the field of organoid culture, and particularly relates to a mixed type gastric cancer organoid culture medium and a culture method and application thereof. Wherein the culture medium comprises a basic culture medium and a specific additive factor; the culture method comprises the following specific steps: treating mixed gastric cancer tissues with tissue digestive juice to obtain mixed gastric cancer primary cells, and culturing with a mixed gastric cancer organ culture medium to obtain mixed gastric cancer organs. The mixed type organ-like form corresponding to the mixed type gastric cancer tissue can be cultured by using the mixed type gastric cancer organ-like culture medium disclosed by the invention. By means of methods such as organoid passage and cryopreservation technologies, the culture success rate of the mixed type gastric cancer organoid is high, sufficient mixed type organoids can be obtained within limited time, an accurate tumor model is provided for individualized tumor therapy, and valuable samples are preserved for scientific research.

The invention belongs to the technical field of cell cryopreservation, and particularly relates to a cell cryopreservation solution and a cell cryopreservation method. The cell freezing medium comprises the following components in percentage by volume: 95% of fetal calf serum and 5% of dimethyl sulfoxide. The temperature conditions for cryopreserving the cells by adopting the cell cryopreservation liquid are as follows: standing for 90 minutes at 4 DEG C, then standing for more than 12 hours or overnight at-70 DEG C, and then immersing into liquid nitrogen for long-term storage. According to the cell cryopreservation liquid and the cell cryopreservation method provided by the invention, the problems that a traditional cell cryopreservation technology and an improved cell cryopreservation box are tedious in operation and low in cell resuscitation success rate, and the improved cell cryopreservation liquid is relatively high in cost are solved, and the cell cryopreservation liquid is simple in ingredient, simple in operation, low in cost and high in cell survival rate.

Methods, systems, and compositions are provided for extracting bone marrow cells from bone obtained from deceased donors, for preparing the bone marrow for cryopreservation, and for obtaining desired cells from cryopreserved and fresh bone marrow. In some embodiments, the methods provide cryopreserved compositions having increased viability of bone marrow cells.

The invention discloses a rectal cancer organoid culture kit and a culture method thereof, and particularly relates to the technical field of genetic engineering.The kit comprises a culture medium with specific components, a sample treatment reagent, a passage reagent and a preservation reagent, and the culture medium contains Advanced DMEM / F-12 and multiple factors; the sample treatment reagent can effectively treat tissues; a passage reagent guarantees passage and cryopreservation; and the preservation reagent ensures the cell cryopreservation and resuscitation effects. The culture method covers scaffold and scaffold-free culture processes and comprises the steps of primary culture, subculture, recovery and the like, and the steps are operated finely. The method has the advantages of obvious advantages, fast tissue dissociation, small damage, good cell suspension dispersion, no DMSO toxicity, simple culture, high amplification efficiency, stable passage, wide applicability and high cost performance, and can provide a powerful tool for rectal cancer research, drug screening and the like.

A cryopreservation box transfer device comprises a main body plate; the tray is installed on the main body plate, a first containing groove and a second containing groove are defined by the tray, and the first cryopreservation box and the second cryopreservation box are placed on the tray so that the cryopreservation tubes can be transferred between the first cryopreservation box and the second cryopreservation box through the tube transferring mechanism; the box moving plate is movably arranged on the main body plate along the horizontal plane, the box moving plate and the tray can vertically move relative to each other, and the box moving plate has a box taking state and a box storing state. According to the cryopreservation box transfer device, the structure of the tray is improved, and the box moving plate is arranged, so that the cryopreservation box and the tray can be mounted and separated by moving the box moving plate, the action is few, and the storing and taking speed is high.

The invention provides a porous gel microsphere capable of being cryopreserved as well as a preparation method and application thereof. The method comprises the following steps: preparing a water-phase solution and an oil-phase solution into gel-state micro-droplets, wherein the water-phase solution contains methacrylic acid gelatin synthesized by low-jelly-strength pigskin gelatin; soaking the gel-state micro-droplets in a liquid containing a photoinitiator, and freezing after full permeation, so that water in the gel-state micro-droplets forms ice crystals; and performing ultraviolet irradiation to form gel-state micro-droplets of ice crystals, and performing cross-linking curing to obtain the porous gel microspheres capable of being cryopreserved. The porous gel microspheres support preloading cells, get rid of the time urgency of current preparation and current use, and can be taken in different time and space for cell printing and injection manufacturing; for a patient, one-time extraction, long-term cryopreservation and taking as required can be realized, so that repeated trauma is avoided.

The invention discloses a special-shaped probe multi-point refrigerating system for bronchoscope cryopreservation, and relates to the technical field of medical instruments, the special-shaped probe multi-point refrigerating system mainly comprises a probe, an air pressure sensing execution sleeve and a wireless computer control system, the special-shaped probe on the probe is provided with four dome cylindrical protruding parts, the middle of the special-shaped probe is sunken, and five capillary tubes are arranged in the probe; the five point refrigeration areas correspond to the convex part and the concave part of the special-shaped probe respectively, so that the five point refrigeration areas are independent; an initial pressure value required by pressure reduction and heat absorption of high-pressure gas is calculated, a wireless signal is transmitted by a computer control system, and then the five air pressure sensing execution sleeves release corresponding pressure, so that the temperature of different point positions on the special-shaped probe is different. Meanwhile, the provided multi-point refrigeration system achieves independent adjustment of the temperatures of different point positions in the probe, prevents the probe from touching and frostbite surrounding healthy tissues by mistake, and has a good clinical application prospect.

The invention discloses a construction method of a somatic cell bank, which belongs to the technical field of somatic cell banks, and comprises the following steps: collection and identification of cell strains, culture and passage of the cell strains, cryopreservation and preservation of the cell strains, cell quality control and detection, updating and recovery of the cell bank, and safety management of the cell bank. The construction efficiency of the cell bank is improved by optimizing the steps of cell culture, passage, cryopreservation and the like, the stability and the activity of cells in the long-term storage process are ensured by adopting an advanced freezing technology and a preservation system, a perfect cell bank information management system is established, detailed information of each cell strain is recorded and managed, and the cell bank management efficiency is improved. The traceability reliability of the cells is ensured, a cell bank safety management system is established, and a plurality of measures are adopted to protect cell resources in the cell bank from being lost and damaged.

The utility model provides a cryopreservation device of a seminal fluid cryopreservation tube, which comprises a freezing box used for containing liquid nitrogen; the box cover is used for sealing the opening of the freezing box; the automatic overturning component is arranged in the freezing box and comprises two supports which are oppositely arranged left and right, inclined frames are formed on the supports respectively, and the inclined frames on the left side and the right side define a containing groove used for containing a freezing tube; rollers are respectively arranged on the inclined frames, and the rolling surfaces of the rollers protrude into the placing grooves so as to be in contact with the outer walls of the cryopreservation tubes in the placing grooves; the rollers are rotatably arranged and connected with a driving device, and the rotation directions of the rollers are the same so as to synchronously drive the cryopreservation tube to turn over during rotation, so that the cryopreservation tube can be continuously turned over by 360 degrees in all directions, liquid nitrogen steam evaporated in the freezing box can uniformly fumigate each surface of the semen cryopreservation tube, and the semen cryopreservation effect is improved. The semen in the cryopreservation tube is precooled more uniformly, the precooling effect is better, and the cryopreservation effect of the semen is more ideal.

The utility model discloses a wild animal fresh excrement sample collection and storage device, which comprises a box body, a collection tool, a sampling label device and a controller, the box body is provided with a storage chamber and a refrigeration chamber, the storage chamber is used for placing the collection tool, and the refrigeration chamber is internally provided with a refrigeration system and a temperature sensor; the collection tool comprises a disposable glove, a collection spoon, a disposable collection sleeve and a cryopreservation tube; the sampling label device comprises a mobile device and a label printer. The controller is provided with an interface module, a data storage module, a data management module, a label generation module, a printing control module, a GPS module and a temperature control module. The device is convenient to carry and operate, the freshness and stability of the sample are kept through low-temperature preservation of the sample, the sample quality is ensured, the sample information is accurately recorded through the sampling label system, follow-up analysis and statistics are facilitated, the fresh faeces sample of the wild animals is conveniently collected and stored, and the field research efficiency and the data quality are improved.

The invention relates to the technical field of electronics, in particular to a scanning equipment control process management method based on a custom protocol. According to the method provided by the invention, a secure communication bridge between a Web environment and local hardware is established through interaction between a custom protocol and an operating system registry; a driver and an API interface matched with a configuration file are dynamically loaded by using a local plug-in, so that multi-model scanning equipment is compatible, and the hardware adaptation and maintenance complexity is reduced; original data is converted into standardized data, an atomized transactional data processing pipeline is constructed, and the integrity and traceability of cryopreservation tube information are ensured; finally, a visual interface is dynamically analyzed and rendered based on a Web client, real-time mapping of cryopreservation tube layout is achieved, and operation intuition, sample searching efficiency and user experience are improved.

The invention discloses an RNA fragment extraction method, and relates to the technical field of molecular biology. Comprising the following steps: S1, preparing materials; s2, cell culture: S21, cell resuscitation; s22, performing cell cryopreservation; s23, carrying out cell passage; s3, co-immunoprecipitation, specifically comprising the following steps: S31, binding an antibody to a protein A / G magnetic bead; s32, carrying out cross-linking combination on the antibody; s33, carrying out cell lysis treatment; s34, carrying out manual antigen immunoprecipitation; s4, carrying out RNA extraction on the basis of a TRIZOL method; and S5, carrying out reverse transcription and real-time fluorescent quantitative PCR. By improving the components of the lysis solution and the washing solution, the extraction efficiency and the yield of RNA are improved, and meanwhile, the pollution of impurities is reduced; the usage amount of reagents and experiment steps are reduced, and the experiment cost is reduced.