244 results about "Biological cell" patented technology

The invention discloses a biological cell image classification method and system and a storage medium, and relates to the field of biomedical analysis, and the method comprises the steps: obtaining a to-be-classified biological cell image, employing a pre-trained AlexNet network as a basic feature extractor, and obtaining a one-dimensional feature vector; key feature information is obtained through feature selection and dimension reduction, one-dimensional feature vectors after dimension reduction are mapped to different feature spaces, and different feature representations are generated through reconstruction; constructing a multi-modal feature, converting the multi-modal feature into a feature map by adopting an attention mechanism, and generating a biological cell portrait; a classification model is constructed based on a graph convolutional network, pre-training is carried out by using transfer learning, a biological cell portrait to be recognized is used as input, and the category probability of each biological cell image is acquired. According to the method, deep learning and multi-modal fusion technologies are combined, the biological cell image classification precision is improved through feature reconstruction and transfer learning, and reliable technical support is provided for biomedicine and clinical diagnosis.

A system includes a flow cell and a fluidics system under pressure causing sheath and sample biological fluids to flow. The fluidics system includes a gas bubble remover to remove and eliminate gas bubbles in the sheath fluid. The flow cell receives sheath fluid from the fluidics system, wherein the sample biological fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid. A deflection chamber under the flow cell receives drops of the sample biological fluid and sheath fluid out of the flow cell to selectively deflect one or more of the drops along one or more deflection paths. A droplet deposition unit system in communication with the deflection chamber receives the selectively deflected drops in the stream of the sample biological fluid with the one or more biological cells or particles into one or more containers.

The utility model discloses a biological cell sampler, which relates to the technical field of cell sampling and comprises a fixing plate, one side of the upper surface of the fixing plate is fixedly connected with the lower surface of a motor, the output end of the motor is fixedly connected with one end of a transmission shaft, and one side of the outer circumferential surface of the transmission shaft is fixedly connected with one side of the inner surface of a transmission mechanism. One side of the outer circumferential surface of the transmission mechanism is rotationally connected with one side of the inner surface of the push rod, the other side of the inner surface of the push rod is rotationally connected with one side of the inner surface of the connecting block through a rotating shaft, the lower surface of the connecting block is fixedly connected with one side of the upper surface of the moving rod, and one side of the lower surface of the moving rod is slidably connected with one side of the upper surface of the fixing plate. The position of the fixing ring is fixed through the transmission plate, the swing rod is driven by external force to rotate with the second supporting block as the center, the telescopic rod is driven by the swing rod to horizontally slide on the inner surface of the fixing ring, the effect of moving according to the operation position of the sampler is achieved, and the problems that a traditional sampler needs to be held by hand, and fatigue is easily caused after long-time operation are solved. And hand shaking is avoided.

A photocatalytic sterilization technology is a technology for killing microorganisms such as bacteria, viruses and the like by utilizing active substances generated by a specific material under an illumination condition. The technology mainly depends on the performance of photocatalytic materials, especially semiconductor materials such as titanium dioxide (TiO2). When the photocatalyst is irradiated by light with a specific wavelength, electrons in the material are transited from a valence band to a conduction band to form electron-hole pairs. The electrons and holes have very high chemical activity, can react with water or oxygen to generate free radicals with strong oxidizing capacity, and can destroy cell walls or cell membranes of microorganisms to cause leakage of substances in cells, so that the sterilization effect is achieved. The invention provides a TiO2 (at) Ag / Ti-MOF functional material aiming at the problems of low light utilization rate and insufficient reaction active sites of a TiO2 photocatalytic material. According to the TiO2 (at) Ag / Ti-MOF functional material, Ag-MOF is constructed on the basis of a Ti-MOF framework, controllable slow release of Ag ions is achieved, and the long-time effective sterilization effect is achieved.

The utility model relates to the technical field of biology, and discloses a biological cell staining device, which comprises a base, a protective shell and a plurality of liquid injectors, the upper end of the base is fixedly connected with a support plate, the upper part of the front end of the support plate is fixedly connected with a fixed plate, the lower end of the fixed plate is rotatably connected with a plurality of movable screw rods, and the movable screw rods are fixedly connected with the protective shell. The outer walls of the multiple movable lead screws are sleeved with movable sleeves in a threaded mode, the lower ends of the multiple movable sleeves are fixedly connected with extrusion plates, the positions, close to the multiple movable sleeves, of the lower end of the fixing plate are fixedly connected with limiting sleeves, and the multiple movable sleeves are slidably arranged in the corresponding limiting sleeves. According to the device, when liquid dropping and dyeing are carried out, the movable lead screw can be rotated by rotating the hand wheel, the movable sleeve and the extrusion plate are pressed downwards through the arranged limiting sleeve, then the liquid injector starts to carry out stable liquid dropping on the glass slide, and accurate quantitative liquid dropping is achieved in cooperation with the arranged scale marks.

The invention relates to the technical field of biological cell culture sampling, and provides a biological cell culture sampling device which comprises a device shell, a supporting frame is fixedly welded to the top end face of the device shell, and a servo motor and a fixed handle are fixedly welded to the bottom end face of the interior of the supporting frame. An output shaft of the servo motor is rotationally connected with the bottom of the device shell and the bottom of the supporting frame, a first circular gear is welded and fixed to the output shaft of the servo motor, a second circular gear is connected to the first circular gear in a meshed mode, a threaded rod is welded and fixed to the second circular gear, and the threaded rod is fixedly connected with the supporting frame in a meshed mode. The top end of the threaded rod is rotationally connected to the top end face of the interior of the device shell. By means of the technical scheme, the problems that in the prior art, a sampling device for biological cell culture cannot conveniently and accurately adjust the sampling amount according to the actual situation, and meanwhile, the sampling position of each sampling assembly cannot be conveniently adjusted according to the size of a culture dish are solved.

The invention discloses a preparation method of quick-frozen sterilized onion dices soaked in saline water, and relates to the field of food processing. The making method of the quick-frozen sterilized onion dices soaked in saline water comprises the steps of primary treatment, blanching, machine cutting, saline water disinfection and quick freezing. According to the preparation method of the quick-frozen salt-water-soaked sterilized diced onions, microorganisms on the surfaces of the diced onions can be effectively killed in a salt-water-soaking sterilization manner, so that the sanitation and safety of the product are ensured. Sodium chloride in the saline water can destroy cell wall structures of microorganisms and inhibit growth and reproduction of the microorganisms, so that the sterilization effect is achieved. By adopting a four-stage gradient freezing mode, fine ice crystals can be formed, damage to cell structures of the diced onions is reduced, and the nutritional ingredients and taste of the diced onions are kept to the maximum extent.

The invention relates to a device (10) for manipulating biological cells, the device (10) comprising: at least one container (22) for cultivating biological cells, the container (22) having an interior space (11), and at least one electrode (14) for manipulating the biological cells, wherein the device (10) comprises at least one separator layer (20), the at least one separator layer (20) being arranged at least between the at least one electrode (14) and the interior space (11) such that the at least one electrode (14) is arranged outside of the interior space (11). The invention further relates to a method (300) for manipulating biological cells. The invention provides a device (10) and a method (300) for manipulating biological cells that avoids the degradation of the cells in containers (22) during the manipulation of the cells with electric fields.

Disclosed herein are devices for encapsulating biological cells and are suitable to be implanted into a subject. In different aspects of the disclosure, the devices may comprise a plurality of polymer layers. In one aspect, a device comprises a first polymer layer and a second polymer layer. In some cases, the first polymer layer may be a nanoporous polymer layer. In some cases, the second polymer layer may be a macroporous polymer layer. The first and second polymer layers may define a lumen for enclosing a population of cells. The devices may be used to transplant cells producing therapeutic agents into a subject (e.g., for the treatment of a disease).

Provided herein are devices and methods for improved oxygenation of biological cells transplanted into a biological subject. The devices and methods may be used to promote vascularization to a transplantation site of the biological subject, and / or may be used to improve survival and functionality of cells contained within an encapsulation device.

The invention discloses a method for generating an enhanced image of a bright-field microscope, and the method comprises the steps: simulating an optical imaging model of optical Zernike phase contrast microscope imaging through a computer system based on the optical imaging principle of a conventional optical Zernike phase contrast microscope; extra hardware such as an annular diaphragm and an annular phase plate does not need to be added in an illumination light path and an imaging light path of the bright-field microscope, enhancement of the phase contrast of a bright-field digital microscopic image is achieved, energy loss of an illumination optical system is avoided, and the design and manufacturing cost of the microscopic optical system is greatly reduced; the optical Zernike phase contrast microscope can be universally used for different bright field microscopes, halo artifacts caused by interference effects in a traditional optical Zernike phase contrast microscope do not exist, finer structural features in biological cells can be distinguished, the resolution of the optical Zernike phase contrast microscope can be close to the resolution limit of objective lens design, and the technical requirements for users are greatly reduced.

The invention relates to the technical field of test tube cleaning and disinfection, and discloses a device for cleaning and disinfecting test tubes after biological cell engineering cultivation, the device comprises a bottom frame, through a designed driving motor and a threaded rod, a placing table for placing test tube bodies can be driven to transversely slide; the placing table drives the test tube body to move into the disinfection bin, the flushing bin, the scrubbing bin and the drying bin, and finally returns to the initial position to be irradiated and disinfected by the disinfection lamp, multiple functions of disinfection, flushing, scrubbing, drying and the like are integrated, the cleaning and disinfection process of the test tube can be completed in a one-stop mode, time and space are saved, meanwhile, manual operation is effectively reduced, and the working efficiency is improved. The cleaning and disinfecting efficiency and consistency are improved, the time-consuming and labor-consuming operation that an operator needs to soak, brush, wash and disinfect the test tubes one by one is avoided, and the problem that the cleaning and disinfecting effects are uneven due to the difference between different operators is solved.

The utility model provides a biological cell dyeing device and relates to the field of biological cells. Comprising a base, a fixing device is arranged on the upper surface of the base and comprises a sliding plate, the upper surface of the sliding plate is fixedly connected with the fixed end of a first electric telescopic rod, the telescopic end of the first electric telescopic rod is fixedly connected with the middle of the lower surface of a concave frame, and the interior of the upper surface of the concave frame is fixedly connected with one end of a spring; the other end of the spring is fixedly connected with the lower surface of the fixing frame. According to the biological cell dyeing device, through the synergistic effect of a sliding plate, a first electric telescopic rod, a concave frame, a spring and a fixing frame, biological cells can be conveniently dyed, when the biological cells need to be dyed, a cell slide is placed on the fixing frame, the cell slide is fixed through the fixing frame, and the spring plays a buffering role; a first electric telescopic rod is convenient for a worker to quickly take the slide, so that the cell slide is fixed.

The invention discloses a mandarin fish medullary tissue cell line and a construction method and application thereof. The invention belongs to the technical field of aquatic organism cells, and discloses a mandarin fish medulla oblongata cell line MFSB, which is preserved in China Center for Type Culture Collection on December 4, 2024, has a preservation number of CCTCC NO: C2024376, and is named as mandarin fish medulla oblongata cell line MFSB. The construction method comprises the steps of primary culture of mandarin fish medulla oblongata tissue cells, subculture, cryopreservation and resuscitation of the cells and the like, and the mandarin fish medulla oblongata cell line MFSB has the advantages of stable subculture, strong cell line division ability, fast growth speed and good stability, can be used as a cell model or an experimental tool to be applied to scientific research and animal genetic breeding, and has wide application prospects. The probe has broad-spectrum sensitivity to mandarin fish infectious spleen and kidney necrosis virus, mandarin fish double-segment RNA virus, mandarin fish frog iridovirus and mandarin fish rhabdovirus, and can be well applied to separation, culture and detection of mandarin fish virus and research and development of vaccines.

Photocatalytic sterilization technology is a technology that uses active substances generated by specific materials under light conditions to kill bacteria, viruses and other microorganisms. This technology mainly relies on the performance of photocatalytic materials, especially semiconductor materials such as titanium dioxide (TiO2). When the photocatalyst is irradiated by light of a specific wavelength, the electrons in the material transition from the valence band to the conduction band, forming electron-hole pairs. These electrons and holes have high chemical activity and can react with water or oxygen to generate free radicals with strong oxidizing ability, which can destroy the cell wall or cell membrane of microorganisms, causing the leakage of intracellular substances, thereby achieving the effect of sterilization. The present application aims to solve the problems of low light utilization rate and insufficient active sites of TiO2 photocatalytic materials, and proposes a TiO2@Ag / Ti-MOF functional material. The TiO2@Ag / Ti-MOF functional material is based on the Ti-MOF skeleton to construct Ag-MOF, realizing the controlled slow release of Ag ions and having a long-term effective sterilization effect.

Systems and methods for encapsulating cells in hydrogel tubes for cell growth within hydrogel tubes are described. In aspects, a system includes a plurality of peristaltic pumps and a plurality of pulse dampeners to provide oscillatory-controlled flow rates of each of a cell solution, a hydrogel solution, and a cross-linking solution to an extruder configured to form hollow hydrogel tubes having suspended biological cells in an interior

The utility model relates to the field of microscopes, in particular to a microscope and a microscope objective. The microscope objective comprises a first lens, a second lens, a third lens and a fourth lens which are sequentially and coaxially arranged from the image space to the object space. The first lens is a negative lens, a second lens, a third lens and a fourth lens; the focal length of the microscope objective is f, and the focal lengths of the first lens, the second lens, the third lens and the fourth lens are f1, f2, f3 and f4 in sequence; wherein the result is-3.0 lt; f1 / flt; -2.5,-2.5; 2.0 lt; f2 / flt; 2.8); 101t; 101t; f3 / flt; 50; 1.2 1t; f4 / flt; and 1.5. The microscope objective can meet the requirements of low conjugate distance, low processing cost and long working distance while obtaining high-resolution imaging performance, and is suitable for being matched with a thick cover glass to complete automatic biological cell detection.

The invention discloses a multilayer imaging method for terahertz near-field system tomography, and belongs to the technical field of terahertz near-field imaging. Aiming at the problem that internal depth quantitative calibration is difficult to realize in existing terahertz near-field three-dimensional imaging, the method proposes that a signal with deepest internal imaging is determined through primary scanning, then multiple data processing is carried out through secondary or multiple scanning, bias voltages of different acquired signals are respectively adjusted under the same magnification times, and the internal depth quantitative calibration is realized in the terahertz near-field three-dimensional imaging. The offset values are arranged in an arithmetic progression, thereby obtaining imaging data representing different linear depths. According to the method, multilayer imaging of internal depth calibration can be realized, a three-dimensional tomographic image with quantitative depth information is formed, and the method is suitable for observation of internal structures of biological cells and nondestructive testing of internal defects of materials.

The present invention relates to a method of preparing a microbial cell product, the method comprising: providing an aqueous suspension comprising microbial cells; subjecting said suspension to mechanical cell dissociation at a temperature in the range of 15 DEG C to 35 DEG C, preferably at a pH in the range of 9 to 11, to obtain an aqueous suspension comprising dissociated microbial cells; separating the suspension to provide an extract rich in small cell debris and an extract rich in large cell debris; and combining at least a portion of the extracts to provide the microbial cell product. The invention further relates to a microbial cell product obtained or obtainable by said method. The invention further relates to the use of said products in food products, for example as a replacement for egg white; in animal food; and / or use in cosmetic formulations.

The present invention discloses a multifunctional oscillating culture integrated shaker and method based on cell culture, which relates to the technical field of enzyme biological cells. A split oscillating platform for placing containers is configured inside the body. The oscillating platform is composed of multiple oscillating plates, and clamping members are configured on the upper part of the oscillating plates to clamp the containers. A driving module connected to the oscillating platform is configured inside the body. The driving module includes an oscillating unit and a connecting unit. The connecting unit connects the oscillating unit and the oscillating plates. The connecting unit is configured with two transmission states for adjacent oscillating plates through a differential, and the differential is configured to drive multiple oscillating plates to oscillate at the same speed or at different speeds by the driving module. In this application, the oscillating platform is split into multiple oscillating plates. When oscillating enzyme biological cells, the enzyme biological cells that require different oscillating parameters are placed on different oscillating plates, and multiple enzyme biological cells can be oscillated in the same shaker through oscillating plates with different oscillating frequencies.

The present invention belongs to the technical field of photothermal materials, and specifically relates to a thorn-shaped photothermal anode material and its application in sterilization in an electro-Fenton system. The thorn-shaped photothermal anode material provided by the present invention has a nano-thorn-like structure, which has a capture and puncture effect on cells, and can assist the free radicals generated by the electro-Fenton sterilization system to enter the microbial cells and react with cellular substances, thereby improving the utilization efficiency of hydroxyl radicals and enhancing the sterilization and disinfection effect; further, the thorn-shaped photothermal anode material has oxygen vacancies, which can serve as active centers to activate hydrogen peroxide to produce hydroxyl radicals, reduce the amount of reagent added, reduce iron sludge formation, and improve the operating efficiency of the system. The application of the thorn-shaped photothermal anode material to the construction of an electro-Fenton sterilization system can significantly improve the sterilization efficiency.

The invention discloses a mouse primary cardiac endothelial cell extraction method, and belongs to the technical field of biological cell extraction. The method comprises the following steps: step 1, obtaining pure myocardial tissues; 2, the treated heart tissue is transferred into an intelligent tissue grinding system to be ground; step 3, transferring the ground heart tissue into a temperature-controlled enzymolysis reactor, automatically monitoring the digestion process, and terminating the reaction when a preset digestion degree is reached; step 4, carrying out primary cell separation, carrying out magnetic separation by using anti-CD31 magnetic beads, and specifically enriching heart endothelial cells; 5, transferring the purified endothelial cells to an intelligent microenvironment culture system, and automatically optimizing a culture medium replacement period to adapt to a cell metabolism state; and step 6, performing multi-dimensional quality evaluation on the endothelial cells, wherein the multi-dimensional quality evaluation comprises marker expression analysis, in-vitro lumen formation experiment, AI-assisted morphological analysis and digital quality scoring.

The application relates to the field of sewage treatment, and discloses a sulfur autotrophic denitrification biofilter composite filler and a preparation method thereof. The method comprises the following steps: carrying out a silane hydrolysis reaction on a silane coupling agent containing a mercaptan group to prepare a hydrolysis solution, and then adding hydroxylated polystyrene microspheres into the hydrolysis solution to carry out a grafting reaction and prepare the sulfur autotrophic denitrification biofilter composite filler. The method grafts mercaptan groups to the surface of the polystyrene microspheres by using the silane coupling agent containing the mercaptan group. The mercaptan groups can specifically combine with sulfur transport proteins on the cell membrane of sulfur autotrophic denitrification microorganisms, so that the sulfur autotrophic denitrification microorganisms can selectively colonize on the surface of the polystyrene microspheres, competition between other microorganisms and the sulfur autotrophic microorganisms can be avoided, the colonization stability of the sulfur autotrophic microorganisms is improved, and the biofilm formation efficiency is improved.

The invention provides a microbial desalination system and device for saline-alkali soil improvement, and relates to the technical field of microbial washing and dewatering treatment.The microbial desalination system comprises a washing and dewatering treatment system which comprises a mechanical grid and a flocculation reaction tank and is used for removing suspended solids (silt and organic particles), reducing turbidity and improving subsequent microbial activity; and the microbial water purification system comprises a sequencing batch reactor and a microbial agent adding device, is equipped with an aeration system, and adsorbs or converts salt by using salt-tolerant microorganisms. According to the scheme, when the washing water is desalted through microorganisms, a PH regulator and glycerin are added into the washing water in the reaction tank firstly, the washing water with the high PH value is prevented from damaging a lipid bilayer structure of a microbial cell membrane by adjusting the PH value of the washing water, and therefore the extracellular environment is stabilized; and the compound can be actively absorbed into cells by microorganisms, the osmotic pressure in a high-salt environment is balanced, and cell dehydration is prevented.

A self-organizing swarm robot system and control method based on cellular soft and hard mechanisms are disclosed. The system includes several individual robots. Each robot includes an upper shell, a middle support, a lower support chassis, electrically driven legs, a magnetically connected actuator, a PCB control board, and light-emitting elements. The PCB control board is equipped with an ESP-NOW wireless communication module, a light sensor, and a multi-pose sensor. The magnetically connected actuator includes static and dynamic actuators alternately distributed along the circumference. This invention can simulate the adhesion force regulation and morphological expansion and contraction mechanism between biological cells, achieving dynamic control between swarm robots under mechanical action. It exhibits high flexibility and fast response speed, enabling multi-degree-of-freedom movement and posture adjustment in complex environments. By using ESP-NOW wireless communication technology in conjunction with light sensors and multi-pose sensors to construct a high-precision distributed sensing and control system, it supports the self-organization, collaboration, and task execution capabilities of swarm robots in weak information environments.

The invention discloses a tyrosinase near-infrared fluorescent probe as well as a preparation method and application thereof, and belongs to the technical field of analytical chemistry. According to the technical scheme, the tyrosinase near-infrared fluorescent probe is characterized in that the structural formula of the tyrosinase near-infrared fluorescent probe is shown in the specification. The invention further specifically discloses a preparation method of the tyrosinase near-infrared fluorescent probe and application of the tyrosinase near-infrared fluorescent probe to selective detection of tyrosinase in a water environment, a biological cell system and living organisms. The near-infrared fluorescent probe disclosed by the invention has the advantages of fluorescence emission in a near-infrared region, good selectivity, high sensitivity and the like.

The application discloses a system and method for controlling liposome drug directional delivery based on a spectrum space phase gradient light field, and belongs to the technical field of light field regulation of biological cells; in the application, quasi-plane waves are irradiated onto a spatial light modulator, the amplitude of a target light field is embedded into a phase by using Fourier expansion, and then a holographic template of the reconstructed light field is generated; the holographic template is loaded onto the spatial light modulator, and after filtering through a 4f system, the light beam is projected to an objective lens; on the focal plane of the objective lens, with the help of the existence of the phase gradient, the drug-loaded liposome is transported along a preset track in a directional manner, and is captured by an intensity gradient optical trap at the end of the track; the application can design any drug delivery track according to requirements, and without moving the equipment platform, the two manipulations of any track delivery and target capture can be realized through a single light beam, and the convenience and efficiency of drug directional delivery are improved.

The invention discloses a novel molecular chaperone-mediated autophagy (CMA) activating agent, relates to the technical field of biological cells, and is characterized in that the novel molecular chaperone-mediated autophagy activating agent is a novel anti-tumor drug chidamide, and the novel molecular chaperone-mediated autophagy activating agent is a novel anti-tumor drug chidamide. The chidamide can obviously up-regulate the mRNA and protein level of the key protein LAMP2A in the CMA process. According to the present invention, by constructing a human THP1-LAMP2luciferase reporter gene system, the compound capable of activating the LAMP2 promoter is screened from the bulk drug; through cell level primary screening, secondary screening and animal level verification, an effective CMA activator chidamide is finally determined. A good tool is provided for basic research of CMA, and novel drugs are further researched and developed by taking CMA as a target spot.

A photoacoustic flow cytometry (PAFC) device for the in vivo detection of cells circulating in blood or lymphatic vessels is described. Ultrasound transducers attached to the skin of an organism detect the photoacoustic ultrasound waves emitted by target objects in response to their illumination by at least one pulse of laser energy delivered using at least one wavelength. The wavelengths of the laser light pulse may be varied to optimize the absorption of the laser energy by the target object. Target objects detected by the device may be unlabelled biological cells or cell products, contrast agents, or biological cells labeled with one or more contrast agents.

The utility model discloses a biological cell storage cabinet, which comprises a cabinet body, a baffle plate, a storage frame, a lifting spoon and a cell culture plate, a support block is arranged at the upper end in the cabinet body, the baffle plate is placed in the cabinet body, and the bottom of the baffle plate is attached to the top of the support block. The lifting spoon and the connecting rod are additionally arranged, liquid nitrogen can be conveniently added and transferred by a worker, direct contact between the liquid nitrogen and the storage plate is avoided, the opening used for adding the liquid nitrogen is additionally formed in the baffle, liquid nitrogen can be conveniently added into the lifting spoon, liquid nitrogen splashing is avoided, and the liquid nitrogen at the opening is isolated through the cover plate when the liquid nitrogen storage device is not used. The observation hole for placing the wooden stick is additionally formed in the cover plate, the liquid level height is measured by observing the frosting length of the wooden stick, liquid nitrogen in the observation hole is isolated through the end cover when the cell culture plate is not used, leakage of the liquid nitrogen is further reduced, a plurality of transversely-arranged insertion rods are additionally arranged, when a single cell culture plate is taken and placed, the other cell culture plates cannot be inclined and fall off, and the cell culture plate is convenient to use. The cells are convenient to take and place.