The invention discloses an all-optical three-dimensional scanning
confocal fluorescent
microscopic imaging device and an implementation method thereof. According to the invention, through a
deep learning driven adaptive regulation and control method, CNN is adopted to process
spatial distribution data and dynamically regulate and control a phase hologram and the
light intensity and
phase compensation of a
laser, so that the focal point of exciting light is subjected to aberration-free
axial displacement, a bidirectional parallel
optical scanning track of a two-dimensional scanning
system is optimized, and all-optical three-dimensional scanning is realized; lSTM and TCN are combined to obtain a
time sequence dependency relationship, a
laser light source, an adjustable diaphragm, an electric focus-adjustable lens and a photoelectric
detector are integrally controlled, efficient synchronization and automatic operation and high-speed axial focusing adjustment are realized, errors and
time sequence mismatch are eliminated, optical characteristics of different samples and environmental interference are automatically adapted, and high-quality imaging is kept. The robustness and the applicable scene range of the
system are improved; the method is used for model biological
embryo real-time tracking,
intracellular signal molecule
dynamic visualization,
cell membrane protein migration and aggregation observation and
intracellular organelle interaction tracking.