586 results about "Reproduction" patented technology

The invention provides a digital twinborn monitoring, regulating and controlling method for a copepod bait biological domestication process. The method comprises the following steps: establishing a micro-ecology and population dynamics coupled twinborn model, and defining a monitoring parameter and control object unified term system; collecting multi-source sensing data at an edge end, and performing clock synchronization, signal denoising and state estimation to obtain a micro-ecology, group and water body state joint estimation result; executing parameter online identification based on state estimation, and constructing a safety constraint set of ammonia nitrogen, dissolved oxygen, death rate, environment change rate and the like; the breeding efficiency improvement and the system stability balance are realized through a multi-objective optimization function; and generating a micro-ecological pulse and environment gradual change cooperative control action set according to a solving result, and when a set condition is met, outputting a domestication prescription and terminating the process. Intelligent, low-stress and high-stability regulation and control of a copepod domestication process are realized through digital twin modeling, rolling predictive control and a self-evolution updating mechanism.

The present invention belongs to the ecological field and provides a reservoir ecological regulation method that meets the requirements of fish spawning occurrence and fish egg density, including: obtaining the natural reproduction characteristic information and habitat information of fish species that produce drifting eggs in the regulated target river section, and determining the regulated target fish species; obtaining the first key habitat information required when the spawning occurrence probability of all fish species that produce drifting eggs and the regulated target fish species is not less than a first preset threshold; obtaining the second key habitat information required when the fish egg density of all fish species that produce drifting eggs and the regulated target fish species is not less than a second preset threshold; determining a reservoir ecological regulation plan according to the first key habitat information and the second key habitat information of the river section; and carrying out reservoir ecological regulation according to the reservoir ecological regulation plan. Through the above technical solution, the adverse impact of reservoir regulation operation on the spawning of fish species that produce drifting eggs can be alleviated, which is beneficial to promoting the restoration of fish resources and the protection of biodiversity.

The present invention relates to ARP2 of plant stigma cells; the invention relates to application of Ca < 2 + > transport capacity damage of homologous genes 1 and 1 in breaking through reproduction isolation of plants. According to the method for breaking through the plant reproductive isolation, a foundation is laid for further research on the relationship of small peptides for regulating and controlling reproductive isolation calcium signals through co-receptors, and a simple and effective target is provided for gene modification for breaking through the reproductive isolation.

A female insertion system is provided. The system includes a conformable insert shaped for insertion into one or more structures of a female human that are associated with at least one of reproduction and one or more erogenous zones; one or more sensors, at least one of the sensors included in the insert, and configured to measure and to relay data, wherein the data is acquired on one of a one-time basis and a multiple-time basis included one of a non-continuous period, a continuous period, and an intermittent temporal period; and a processor configured to: control the insert; generate a treatment for one of the structures of the female human; store the data; and based on the data, automatically affect one or more of the structures via the insert.

The invention provides a captive panda oestrus starting prediction method and system based on machine learning, and relates to the technical field of panda breeding, and the method comprises the steps: obtaining original data; isomerous data stream synchronization is carried out according to the original data, and a synchronized spatio-temporal data matrix is obtained; performing nonlinear interaction relation modeling according to the synchronized spatio-temporal data matrix to construct a spatio-temporal dependency relation graph; performing environment constraint feature dimension reduction according to the space-time dependency graph to obtain a low-dimensional state representation vector; performing oestrus state transition probability calculation according to the low-dimensional state representation vector to generate a probability evolution trajectory; and according to the probability evolution trajectory, turning point identification is carried out, and an estrus starting moment prediction value is output. Through collaborative inflection point detection of progesterone gradient characteristics and an ingestion mutation mode in the probability evolution trajectory, an estrus starting moment prediction value is accurately output, and finally the biological interpretability of estrus critical state evolution of captive pandas is improved.

The present invention relates to the fields of biotechnology and plant breeding, specifically providing a method for fixing heterosis in rice using the DMP gene. To address the low fruit set rate and complex operation issues of existing apomictic reproduction systems, the present invention verifies for the first time the haploid-inducing function of the rice genes OsDMP8 and OsDMP13. Furthermore, the CRISPR / Cas9 technology is used to simultaneously knock out the OsPAIR1, OsREC8, OsOSD1, and OsDMP genes, constructing a "MiMe-OsDMP" four-gene editing system. This system provides new ideas for constructing apomictic reproduction systems in rice and offers a new solution for fixing heterosis in rice apomictic reproduction. This system can significantly improve the efficiency and quality of agricultural production, and has significant economic value and broad application prospects.

The invention belongs to the technical field of gene detection and gene molecular breeding, and particularly relates to a meat duck whole genome molecular probe combination based on molecular phenotype screening, a 50K gene chip and application thereof. The molecular probe combination and the gene chip of the marker site combination for meat duck whole genome breeding simultaneously cover 7 representative meat duck varieties and 71 economic characters, have richer polymorphism and higher pertinence in meat duck groups, and are lower in cost and higher in speed compared with high-throughput sequencing detection; the breeding chip is designed according to the growth, feed efficiency, slaughtering, breeding, egg quality and various molecular phenotypes of the meat ducks, and compared with a high-throughput sequencing technology, the breeding chip is higher in seed selection accuracy, has higher breeding value, can be widely applied to breeding genotype detection of the meat ducks, and can be used for detecting the breeding genotypes of the meat ducks. And the method has creative significance in the aspect of meat duck genome selective breeding.

The invention relates to the field of plant breeding, and particularly provides a method for fixing rice heterosis by using an OsZFPP gene. The method comprises the following steps: firstly, constructing an OsZFPP1 gene or OsZFPP2 gene expression cassette driven by an OsECA1 promoter; then, an expression box of rice with three target points of OsPAIR1, OsREC8 and OsOSD1 being knocked out of CRISPR / Cas9 is constructed; integrating the expression cassette into the same vector to transform hybrid rice; screening three-gene homozygous mutation and OsZFPP positive plants, and identifying diploid cloned offspring through flow cytometry and genome sequencing. According to the method, the rice OsZFPP gene is combined with MiMe to provide a novel method for fixing the heterosis of the rice through apomixis, the heterosis of the rice can be successfully fixed through the method, an apomixis system with the high maturing rate can be obtained, and a novel solution is provided for fixing the heterosis of the rice through the apomixis.

The invention provides application of a sperm tRNA (transfer ribonucleic acid) derived fragment 5 'tRF-Glu-CTC in prediction of development potential of assisted reproductive embryos. The expression of a tRNA derived fragment 5 'tRF-Glu-CTC in a sperm sample is detected, the abnormal expression of the sperm specific tRNA derived fragment 5' tRF-Glu-CTC in a DFI increase patient is verified, and the analysis on the correlation with clinical data and reproductive outcome of the patient prompts that the 5 'tRF-Glu-CTC molecular marker has significant correlation with the reproductive outcome, so that the 5' tRF-Glu-CTC molecular marker can be used for detecting the expression of the sperm specific tRNA derived fragment 5 'tRF-Glu-CTC in the DFI increase patient. The embryonic development potential can be effectively predicted, and a biomarker is provided for assisted reproduction treatment. In different experimental groups, the 5 'tRF-Glu-CTC can significantly improve the prediction precision, and shows good prediction performance in different clinical data. The detection method based on the fragment is expected to become a new tool for evaluating the assisted reproduction curative effect, the reproduction treatment scheme is further optimized, and the success rate is improved.

The invention discloses a chilo suppressalis indoor artificial feeding feed and a feeding and reproduction method, and relates to the technical field of insect feeding. By optimizing the proportion of the indoor artificial breeding feed for the chilo suppressalis and the breeding method, the method has the following technical effects that (1) the survival rate of larvae within 15 days reaches 100%, and the survival rate of the larvae is increased; (2) the optimized feed formula can meet the nutritional requirements of larvae in each stage, the taste is good, the feed attraction is strong, and the synchronous development is promoted; (3) the breeding efficiency is improved; (4) the disease and pest risk is reduced, and the disease occurrence rate is lower than 5%; and (5) ensuring the quality of the insect source: the obtained chilo suppressalis insect source is consistent in physiological state and neat in development, the insect body conversion rate reaches 69.6%, and the insect use requirements of large-scale experiments are met.

The invention discloses a green prevention and control method for invasive compositae plants, and particularly relates to the technical field of ecological management, and the method comprises the following steps: S1, sample collection, S2, preparation of a microbial agent, S3, application of the microbial agent, S4, putting of special-feeding insects, S5, planting of mutual restriction plants, and S6, ecological restoration and detection. According to the green prevention and control method for the invasive compositae plants, the physiological structures of the invasive compositae plants are destroyed through the microbial agent, so that the invasive compositae plants are more sensitive to allelochemicals; the growth of the plants is inhibited through the allelopathy of the mutually-restrained plants, meanwhile, the soil environment is improved, microorganism breeding is facilitated, double-inhibition and growth-promoting circulation is formed, and in addition, after the number of invasive plants is reduced through special-feeding insects, a living space is provided for local plants; local plants planted for ecological restoration can provide inhabiting and breeding places for insects, so that insect population stability is maintained, and long-acting biological control is realized.

The invention provides an efficient breeding method of cremastra appendiculata, and relates to the technical field of cremastra appendiculata breeding. The efficient breeding method comprises the steps of soil preparation and land selection, strain laying, wood laying, gap filling, seed dressing, sowing, shading and management. According to the method, the specific method is adjusted, humus soil containing a compound microbial agent is adopted to fill gaps among wood, then sowing is conducted, the germination rate of seeds is increased, sawdust, perlite, coco coir, decomposed organic fertilizer, bamboo charcoal powder and pyroligneous liquor are used for seed dressing before sowing, the air permeability of a matrix is improved, seeds are prevented from being rotten due to accumulated water, and breeding of pathogenic bacteria is inhibited; the water-retaining property of the substrate is increased; and symbiotic bacteria planting and seed metabolism are promoted. The foliar fertilizer containing the garlic extracting solution, the onion leaching solution, the monopotassium phosphate, the boric acid and the urea is adopted, elements needed by growth of the rhododendron simsii are provided, the strong insecticidal and bactericidal effects are achieved, and therefore the incidence rate of diseases and pests is reduced, and the seedling survival rate and the breeding efficiency of the rhododendron simsii are improved.

The invention discloses a sex attractant for bactrocera dorsalis and application of the sex attractant. The invention provides a sex attractant for bactrocera dorsalis. The sex attractant for bactrocera dorsalis comprises ethyl laurate, ethyl myristate, cis-9-hexadecenoic acid ethyl ester and ethyl palmitate. The sex attractant provided by the invention can effectively reduce the population density of male adult bactrocera dorsalis in the field, reduce successful mating of female adult bactrocera dorsalis, and reduce the reproduction rate of bactrocera dorsalis. The sex attractant developed by the invention is a novel bactrocera dorsalis attractant, can enrich compound resources for biological prevention and control of bactrocera pests and promote the development of a pest comprehensive prevention and control strategy towards the directions of high efficiency, economy, greenness and the like, and has very high application value.

The invention belongs to the technical field of aquaculture, and particularly relates to a pearl grouper fry rearing method. According to the method, firstly, after the roes are hatched, the fries are soaked with a bactericide, harmful bacteria on the surfaces of the roes and in a hatching pond can be effectively killed, and a bacteria solution of lactobacillus brevis serves as the bactericide, so that the use of antibiotics is reduced while the growth of pathogenic bacteria is inhibited; the culture pond is divided into 1m * 1m spaces by the partition plates, so that the phenomenon that the fries kill each other or snatch for food due to insufficient spaces is avoided, and uniform growth of the fries is facilitated; culture is carried out in stages according to nutritional requirements of different growth stages, and the Weissella microbial inoculum and the lactococcus lactis microbial inoculum are added to the feed in each stage, so that the intestinal microecological balance of the fry is adjusted, the growth and reproduction of harmful bacteria are inhibited, the intestinal digestion and absorption functions of the fry are enhanced, and the utilization rate of the feed is increased.

The utility model relates to the technical field of ecological devices, and discloses a symbiotic ecological system with balanced microorganism reproduction, filtration and purification, which comprises a culture bed, a purification box arranged on the right side of the culture bed, a first fixed box arranged on the right side of the purification box, and a second fixed box arranged on the right side of the first fixed box, a water treatment mechanism is arranged in the purification box, an ecological expansion mechanism is arranged in the first fixed box, an internal circulation mechanism is arranged in the second fixed box, and the water treatment mechanism comprises a drainage assembly and a treatment assembly. According to the symbiotic ecological system with the microorganism balanced reproduction, filtration and purification functions, in the using process, the ecological core principle of natural balanced reproduction of microorganisms is followed, water in the breeding area can be precipitated, filtered and purified through the arranged water treatment mechanism, the situation that water is smelly is avoided, water does not need to be changed during fish culture, and the water treatment efficiency is improved. The later operation and maintenance cost of the fish tank is reduced, and an ecological scene with continuous ornamental value is realized.

The invention belongs to the field of ecology, and provides a reservoir ecological scheduling method capable of meeting fish spawning occurrence and roe density requirements, which comprises the following steps: acquiring natural reproduction characteristic information and habitat information of fishes laying drifting roes in a scheduling target river reach, and determining the scheduling target fishes; acquiring first key habitat information required when the spawning occurrence probability of all the fishes laying the drifting eggs and the scheduling target fishes is not less than a first preset threshold value; acquiring second key habitat information required when the roe density of all the fishes laying the drifting roes and the scheduling target type of roes is not less than a second preset threshold value; according to the river reach first key habitat information and the river reach second key habitat information, determining a reservoir ecological scheduling scheme; and performing reservoir ecological scheduling according to the reservoir ecological scheduling scheme. By means of the technical scheme, the adverse effect of reservoir dispatching operation on spawning of the fishes laying the drifting eggs can be relieved, and fish resource recovery and biodiversity protection are facilitated.

The invention provides a method for overspeed preparation of gene edited goldfish. The method comprises the following steps: S1, obtaining gobiocypris rarus without primordial germ cells as receptor fish; s2, designing a target spot for the goldfish amh gene, introducing a Cas9 / gRNA complex into a goldfish fertilized egg, performing sequencing verification to obtain a mutant individual, and feeding the mutant individual as a donor fish; s3, taking gonads of donor fish, cutting into pieces, performing enzymolysis digestion, filtering, and performing density gradient centrifugation to enrich donor germline stem cells; s4, transplanting the germline stem cells of the donor fish into the receptor fish body, and feeding to obtain the gene edited goldfish sperms and ova. S5, hybridizing and feeding goldfish amh mutant sperms and ova produced by gobiocypris rarus to obtain gene edited goldfish individuals. According to the invention, gobiocypris gobiocypris is used as a receptor, the cross-subfamily gene edited goldfish is used as a donor, and an efficient, stable and easy-to-popularize method for preparing the gene edited goldfish is provided by utilizing a belly borrowing reproduction technology.

The invention discloses a method for constructing an Asiatic corn borer GBP gene homozygous mutant, which comprises the following steps: amplifying and cloning an Asiatic corn borer GBP gene sequence by using RACE technology, and the nucleotide sequence of the Asiatic corn borer GBP gene is as shown in SEQ ID No.1; two sgRNA target sequences are designed on a second exon of a GBP gene sequence of the ostrinia furnacalis, target sgRNA is synthesized through in-vitro transcription, the target sgRNA and Cas9 protein are fused in vitro to form an RNP mixture, and the RNP mixture is injected into ovum of the ostrinia furnacalis; the method comprises the following steps: carrying out PCR (Polymerase Chain Reaction) amplification on a target spot by using a target spot detection primer, sequencing to confirm a mutant individual, hybridizing and selfing to obtain a GBP homozygous mutant population. The Asiatic corn borer GBP gene homozygous mutant is constructed, so that the reproduction and immune functions of the mutant are remarkably reduced, and reference and research basis are provided for later development and formation of efficient pest biopesticide.

The invention relates to the technical field of biology, in particular to a molecular marker for identifying Holstein cattle with high fecundity and application of the molecular marker. According to the invention, an RBX1 gene is analyzed in cattle 150K genotype chip data, and SNP loci related to first and last hybridization interval characters are found; the method comprises the following steps: establishing a 30x re-sequencing database of high and low reproduction populations for the first time, and autonomously identifying the SNP sites of the high-fecundity Holstein cattle; an important molecular target is provided for the creation of a new gene editing breeding material in the later period, the creation of an autonomous breeding chip in China and the breeding of cattle.

The invention discloses a method for separating, purifying and transplanting spermatogonial stem cells of lateolabrax japonicus, the spermatogonial stem cells of lateolabrax japonicus are separated and purified, marine fish larvae or freshwater fish larvae are used as receptor larvae, and then the spermatogonial stem cells of lateolabrax japonicus are transplanted to abdominal cavities of the receptor larvae. The spermatogonial stem cells obtained through separation are complete in number, structure and function and low in cell clustering rate. After the transplanted spermatogonial stem cells are subjected to fluorescence labeling, the migration and proliferation processes of donor germ cells can be observed in receptor fishes of marine fish fries or freshwater fish fries, so that the receptor fishes with the chimeric germline are obtained. According to the method, through xenotransplantation of the spermatogonial stem cells, a'belly borrowing reproduction 'breeding mode is provided for the marine fishes, a new way for gamete preservation and rapid propagation is provided for the marine fishes with the long reproductive cycle, and a new method and a new thought are provided for genetic improvement and germplasm resource preservation.

The invention relates to the technical field of new silkworm variety breeding, and discloses a method for assisting silkworm variety breeding by parthenogenesis, which comprises the following steps: introducing a parthenogenesis high-incidence material into a target variety, screening individual hybridization recurrent parents retaining target characters or genes, and backcrossing with recurrent parent varieties, excellent individuals with target characters are selected for parthenogenesis homozygous immobilization and bisexual reproduction homozygous immobilization; according to the characteristic that a parthenogenesis high-incidence material is not easy to lose in filial generations due to the parthenogenesis high incidence, the parthenogenesis high-incidence material is hybridized, backcrossed and selfed with a target character variety and a conventional excellent variety, and after backcrossing is completed, parthenogenesis homozygous target character is carried out; target character homozygosis can be realized in a few generations, stable homozygous offspring can be obtained, and the breeding process is expected to be shortened.

The invention is applicable to the technical field of vaccines, and provides a haemaphysalis unguiculata salivary gland multi-component subunit vaccine as well as a preparation method and application thereof, the effective components of the vaccine comprise rHlPDI-2 and rHlPrx soluble recombinant proteins, and the amino acid sequences of the rHlPDI-2 and rHlPrx soluble recombinant proteins are shown as SEQ ID NO.1-2. Starting from salivary gland secretory protein related to tick activity in a host biting process of haemaphysalis unguiculata, special protein of salivary glands of tick species is researched, the recombinant protein vaccine is prepared, survival and reproduction of haemaphysalis unguiculata can be affected, and the prepared multi-component subunit vaccine has an interference effect on biting and passage of haemaphysalis unguiculata and can be used for preparing the multi-component subunit vaccine for preventing and treating haemaphysalis unguiculata. The anti-tick vaccine has a good anti-tick effect, contributes to prevention and treatment of tick-borne diseases, and lays a foundation for development of other tick related vaccines.

According to the method, the pollination problem of an endangered plant, namely the cymbidium serotina, is researched, and in order to improve the pollination rate of the cymbidium serotina and optimally select the pollen collection time, the pollination tool and the pollination time, the invention provides a method for adopting cactus thorns as the pollination tool for the first time, so that the pollination of the cymbidium serotina is facilitated, and the pollination efficiency of the cymbidium serotina is improved. The pollen utilization rate is greatly improved, the breeding number of the precious relict plants is increased, and an efficient pollination method is provided for breeding of the rare and endangered plants.

The invention discloses a method for separating and transplanting spermatogonial stem cells of Chinese sturgeons, and belongs to the technical field of separation of germline stem cells of fishes. The separation method comprises the following steps: washing Chinese sturgeon testis tissues with an L-15 culture medium containing penicillin / streptomycin double antibodies, and carrying out physical shearing, digestive enzyme enzymolysis and screen filtration to obtain a Chinese sturgeon testis cell suspension; performing percoll density gradient centrifugation to obtain high-purity spermatogonial stem cells of the Chinese sturgeons; and dyeing, washing, resuspending and carrying out micro-injection transplantation to enter the gonad primordium reproductive ridge part of the juvenile acceptor fish. According to the physical characteristics of the spermatogonial stem cells of the Chinese sturgeons, the spermatogonial stem cells with high purity are obtained by adopting percoll density gradient centrifugation, and the purpose of abdomen borrowing reproduction is achieved by adopting a microinjection mode, so that the preservation of germ cells of endangered fishes and the reconstruction of artificial populations are realized; and a new technical approach is provided for protecting genetic resources of the Chinese sturgeons and breaking through artificial propagation bottlenecks.

The invention relates to the technical field of microorganisms, in particular to bacillus licheniformis JSF-9 and a complex microbial inoculant and application thereof.The strain JSF-9 is obtained by being separated from healthy cattle rectum contents by a research group, and it is detected that the strain has the good beta-1, 4-glucanase producing capacity, and the beta-1, 4-glucanase producing capacity is high. The antibacterial effect on staphylococcus aureus, salmonella typhimurium and shigella flexneri is achieved; the strain has a good bagasse fermentation capability, for example, after the strain, bacillus altitudinis MLF-1 and bacillus safensis MLL-5 are prepared into a mixed microbial inoculum, the bagasse fermentation capability is better, and verification shows that the complex microbial inoculum can effectively inhibit the reproduction of harmful bacteria, so that the preservation period of fermented feed is prolonged, and the yield of the fermented feed is improved. Animal feeding experiments find that the nutritional value and the disease resistance of the complex microbial inoculant fermented bagasse are remarkably improved, 10-15% of the complex microbial inoculant fermented bagasse is added into basic ration, and the cost-reducing and effect-increasing effects are achieved for feeding Jersey cattle.

The invention provides acinetobacter for culturing prawns in saline-alkali water and application of the acinetobacter. The preservation number of the provided acinetobacter is CGMCC (China General Microbiological Culture Collection Center) No.36066. The acinetobacter soilis strain provided by the invention has remarkable comprehensive application advantages, can still keep higher growth activity and metabolic capability in a low-temperature environment, and continuously plays a water body purification function. Ammonia nitrogen and nitrite nitrogen in a water body can be efficiently degraded, the environment of the aquatic water body is remarkably improved by promoting the nitrogen circulation process, and harm caused by nitrogen pollution is reduced. In addition, the strain can effectively inhibit and prevent reproduction of pathogenic vibrios and reduce the occurrence risk of bacterial diseases of aquatic animals. The strain is high in safety, does not cause poison or stress reaction to bred animals even under the condition of high-concentration use, and has good ecological safety and environmental friendliness.

The invention discloses a real-time monitoring integrated system for health and reproduction conditions of dairy cows, and relates to the field of intelligent animal husbandry, and the system comprises a model construction module, a health abnormity early warning module, a fusion synchronization module, a file establishment module and an interactive feedback module. According to the method, the digital twin of each dairy cow is constructed, so that the whole-process and full-dimension dynamic mapping of the physiological state, the behavior track and the reproduction cycle of the dairy cow is realized, and the management precision and the response efficiency are improved. According to the method, multi-modal behavior characteristics such as tail vibration amplitude, step number change rate and sound frequency are combined, and a deep learning model is fused for intelligent reasoning, so that the misjudgment rate and the omission ratio are effectively reduced, and the disease risk and the estrus window period are accurately identified. Based on physiological, behavioral and historical health data, quantifiable health scores are generated for each dairy cow, and a trend chart is formed to assist in finding potential diseases, monitoring a recovery process or identifying superior and inferior breeding individuals.

The invention discloses application of cordyceps javanica Bd01 and a dispersible oil suspending agent thereof to prevention and control of spodoptera frugiperda, and relates to the technical field of biology. The research finds that the cordyceps javanica Bd01 has a strong infection lethal effect on larvae of spodoptera frugiperda at all ages, the pupation rate and eclosion rate of the larvae can be remarkably reduced, and the breeding cycle of pests can be effectively blocked. The invention further develops a dispersible oil suspending agent, and the suspending rate is effectively improved through formula optimization. The dosage form takes soybean oil as a dispersion medium, does not contain harmful solvents such as methylbenzene, is free of dust pollution and is friendly to operators and the environment, and ascorbic acid is added to improve the ultraviolet resistance of spores and prolong the effective period of the pesticide effect. In addition, the preparation also has a certain control effect on caffeinidae, is wide in application scene, provides a new scheme for green control of spodoptera frugiperda, solves the problems of chemical pesticide resistance and residue, and accords with the development requirements of green agriculture.

The invention relates to the technical field of pollutant assessment methods, in particular to an ecological risk assessment method for the effect of pollutants on aquatic organism populations. According to the ecological risk assessment method for the effect of the pollutants on the aquatic organism population, breeding and survival data of the aquatic organisms are obtained on the basis of individual multi-generation toxicity exposure experiments, the matrix model in the population ecological risk assessment model is utilized to predict the toxicity effect of the population level, and the ecological risk of the pollutants on the aquatic organism population effect is evaluated. Further predicting the toxic effect of pollutants on the individual level and population level of organisms under a long-term exposure condition, comprehensively evaluating and determining the percentage of decrease of the intrinsic growth rate of the population in combination with an individual toxic effect threshold value, deducing the toxicity threshold value of the population level, and performing risk evaluation on the population level. Meanwhile, the ecological risk of the population level is quantitatively represented by using the loss expectation of the population intrinsic growth rate. The risk evaluation method provided by the invention can provide data support for further formulating a corresponding environmental protection benchmark.

The invention discloses a method for regulating out-of-season breeding of large yellow croakers bred in autumn. The method is characterized in that spawning in spring and summer is achieved through a three-stage technical system of biological safety prevention and control, precise environment regulation and control and reproductive physiological intervention. The method specifically comprises the following steps: 1) detecting trypanosomes; 2) parent fish screening; (3) parent fish rearing; 4) artificial propagation; 5) artificial incubation; and 6) seedling cultivation. According to the method, the out-of-season spring and summer breeding technology breakthrough of breeding the large yellow croaker in autumn is successfully achieved through nutrient enrichment, accurate temperature parameter regulation and control, gradual illumination regulation and reasonable hormone treatment. According to the technology, an adjustable reproduction synchronization scheme is provided for cross breeding between a spring reproduction group and an autumn reproduction group, and the technology has important application value for germplasm innovation of the large yellow croaker.