A method of 
mass production 
grease tung seed sprout through 
tissue culture, comprising the following steps: 1. inoculating the stem tip into the culture medium added with 
corn starch with 20-40% by weight: MS+6-BA 0.5í½1.5mg / L+NAA 0.2í½2mg / L+
sugar 20-40g / L, solidifying with 
agar of 3-5%, culturing with light, with pH being 5.8 to 6, temperature in the culture room being 26í‚2 Deg C, 
light intensity being 1000-2000
lux, lighting hour being 10-14 hours every day, culturing for 20-28 days to form 
callus. 2. 
Cutting the 
callus and transferring it to the same culture medium for successive culture, and getting the 
tissue culture bud. 3. inoculating the 
tissue culture bud of bred multi-period and in 
mass to the following root-growing culture medium to get the tissue culture sprout: IBA0.3í½0.6mg / L+MS, the root growing out 10-20 days later. 4. Replanting the tissue culture sprout out of 
bottle. The invention is characterized by the solution of problem of fast breeding 
grease tung using tissue culture, short period, low cost, and capability of meeting demand of large-area cultivating 
grease tung, it provides a high effective way for the grease tungartificial breeding, germ-free sprout producing and species of high quality breeding.