A method of mass production grease tung seed sprout through tissue culture, comprising the following steps: 1. inoculating the stem tip into the culture medium added with corn starch with 20-40% by weight: MS+6-BA 0.5í½1.5mg/L+NAA 0.2í½2mg/L+sugar 20-40g/L, solidifying with agar of 3-5%, culturing with light, with pH being 5.8 to 6, temperature in the culture room being 26í‚2 Deg C, light intensity being 1000-2000lux, lighting hour being 10-14 hours every day, culturing for 20-28 days to form callus. 2. Cutting the callus and transferring it to the same culture medium for successive culture, and getting the tissue culture bud. 3. inoculating the tissue culture bud of bred multi-period and in mass to the following root-growing culture medium to get the tissue culture sprout: IBA0.3í½0.6mg/L+MS, the root growing out 10-20 days later. 4. Replanting the tissue culture sprout out of bottle. The invention is characterized by the solution of problem of fast breeding grease tung using tissue culture, short period, low cost, and capability of meeting demand of large-area cultivating grease tung, it provides a high effective way for the grease tungartificial breeding, germ-free sprout producing and species of high quality breeding.