A method of
mass production
grease tung seed sprout through
tissue culture, comprising the following steps: 1. inoculating the stem tip into the culture medium added with
corn starch with 20-40% by weight: MS+6-BA 0.5í½1.5mg / L+NAA 0.2í½2mg / L+
sugar 20-40g / L, solidifying with
agar of 3-5%, culturing with light, with pH being 5.8 to 6, temperature in the culture room being 26í‚2 Deg C,
light intensity being 1000-2000
lux, lighting hour being 10-14 hours every day, culturing for 20-28 days to form
callus. 2.
Cutting the
callus and transferring it to the same culture medium for successive culture, and getting the
tissue culture bud. 3. inoculating the
tissue culture bud of bred multi-period and in
mass to the following root-growing culture medium to get the tissue culture sprout: IBA0.3í½0.6mg / L+MS, the root growing out 10-20 days later. 4. Replanting the tissue culture sprout out of
bottle. The invention is characterized by the solution of problem of fast breeding
grease tung using tissue culture, short period, low cost, and capability of meeting demand of large-area cultivating
grease tung, it provides a high effective way for the grease tungartificial breeding, germ-free sprout producing and species of high quality breeding.