164results about How to "Short cycle" patented technology

The invention discloses a primer used for gene knockout, which comprises five groups of primer, and can be respectively shown in a SEQ NO:1, a SEQ NO:2, a SEQ NO:3, a SEQ NO:4, a SEQ NO:5, a SEQ NO:6, a SEQ NO:7, a SEQ NO:8, SEQ NO:9 and a SEQ NO:10. The invention also discloses an application of the primer in gene knockout aspect. The invention also discloses a conditional gene knockout method based on a CRISPR/Cas9 technology. The method can performing condition specificity, space-time specificity, and medicine-induced type gene modification; harm due to other cells can be reduced, function of constitutive expression gene in a specific tissue can be researched; tissue and space specificity gene knockout or induction type gene knockout can be realized by only using Cas9 tool mice; test period is short, and time and cost are saved.

The invention discloses a gene polymorphic marker of C-type lectin of portunus trituberculatus. The gene polymorphic marker comprises four SNP (Single Nucleotide Polymorphism) sites of a gene sequence of the C-type lectin of portunus trituberculatus and deficiency of a triplet-base. SNP at 205bp of a fourth exon is associated with susceptibility/resistance of vibrio alginolyticus of portunus trituberculatus, and is named as T/C E4-205. The invention correspondingly discloses a method for obtaining a molecular marker of the C-type lectin associated with anti-vibrio alginolyticus of portunus trituberculatus and a method for association analysis. According to the invention, the molecular markers associated with diseases are obtained through modern molecular biological techniques and a method for quickly identifying SNP is established. The method has the advantages of high throughput, low cost, sensitivity and high specificity, truly realizes closed tube operation, can be used for selecting advantageous genotype individuals as parents by marker assisted selection (MAS), and lays an important foundation for culturing disease-resistant strains of portunus trituberculatus.

The invention discloses near-full-spectrum fluorescent nanocarbon dots and a preparation method thereof and belongs to the field of carbon nano-luminescence materials. The wavelength of an emission peak of the carbon point synthesized by the method is 470nm-700nm; the fluorescent emission peak of the carbon point almost has no change along with the change of the emission wavelength; the carbon dots have the characteristics of high fluorescence intensity and stable quantum yield. The synthesis and preparation method comprises the following steps: adding carboxyl-containing organic acid and organic amine into a certain amount of deionized water according to a mole ratio of (1 to 1)-(1 to 0), uniformly stirring and then transferring the deionized water to a hydrothermal reactor, carrying out hydrothermal reaction, then naturally cooling the hydrothermal reactor to room temperature and preparing a carbon dot aqueous solution. According to the near-full-spectrum fluorescent nanocarbon dots and the preparation method of the carbon dots, the raw materials used for synthesizing the carbon dot are wide in sources and low in cost, and are easily available and almost non-toxic; the carbon dot synthesizing period is short; the carbon dot synthesizing equipment is simple.

The invention discloses a method for fast producing methane by utilizing excess sludge, specifically comprising the steps of: (1) pretreating excess sludge; (2) alkaline hydrolysis: releasing organic matters in the excess sludge into a water phase through adopting a single-stage alkaline hydrolysis or a two-stage alkaline hydrolysis, wherein the SCOD (Soluble Chemical Oxygen Demand) of the water phase achieves 2000-10000mg/L. (3) production of methane through anaerobic digestion: inoculating 10%-60% by volume of anaerobic granular sludge in an anaerobic reactor, controlling the volume load at 0.30-0.75kg COD/(m<3>.d), performing methane production reaction within 72h, wherein the methane production capacity achieves 200-400mLCH4/gCOD. The method has the advantages that the processes of firstly performing alkaline hydrolysis on the excess sludge so as to enable the organic matters to be liquidified and then performing liquid-state digestion to produce methane are fast to start, short in reaction period, stable in methane productivity, not only can energy be recovered, but also the stabilized treatment of the sludge can be realized, and remarkable and environment benefits can be both achieved.

The invention discloses a device for automatic voice synthesis. The device comprises an extracting module, an enhanced detection module, a comprehensive labeling module, a verification screening module and a voice synthesis module. The extracting module is used for automatically extracting voice data out of voice communication; the enhanced detection module is used for automatically distinguishing voices, in the voice data, of more than one person, classifying the distinguished voice data, and dynamically updating the classified voice data; the comprehensive labeling module is used for automatically labeling the classified voice data, and generating labeled information; the verification screening module is used for screening out the voice data which accord with predetermined criteria, and generating corresponding synthetic models through model transformation; the voice synthesis module is used for outputting voices of corresponding users according to the synthetic models. The device for automatic voice synthesis has the advantages of being short in period, low in cost and capable of achieving automatic accumulation.

The invention discloses a preparation method for a graphene-ceramic composite material, which comprises the following steps: magnesium powder and ceramic powder are uniformly mixed, so that mixed powder is obtained, the mixed powder is burned in carbon dioxide gas to react, so that graphene-ceramic composite powder is obtained, and the graphene-ceramic composite powder is shaped and sintered, so that the graphene-ceramic composite material is obtained. The preparation method disclosed by the invention has the characteristics of simple process, short preparation period, low cost and the like, and the prepared graphene-ceramic composite material has the characteristics of uniform component structure, excellent properties and the like. The preparation method utilizes the characteristic of in-situ deposition combination in the gas-solid reaction between magnesium and carbon dioxide to realize the uniform mixing and dispersion of graphene and the ceramic powder on a microscale.

A method of mass production grease tung seed sprout through tissue culture, comprising the following steps: 1. inoculating the stem tip into the culture medium added with corn starch with 20-40% by weight: MS+6-BA 0.5í½1.5mg/L+NAA 0.2í½2mg/L+sugar 20-40g/L, solidifying with agar of 3-5%, culturing with light, with pH being 5.8 to 6, temperature in the culture room being 26í‚2 Deg C, light intensity being 1000-2000lux, lighting hour being 10-14 hours every day, culturing for 20-28 days to form callus. 2. Cutting the callus and transferring it to the same culture medium for successive culture, and getting the tissue culture bud. 3. inoculating the tissue culture bud of bred multi-period and in mass to the following root-growing culture medium to get the tissue culture sprout: IBA0.3í½0.6mg/L+MS, the root growing out 10-20 days later. 4. Replanting the tissue culture sprout out of bottle. The invention is characterized by the solution of problem of fast breeding grease tung using tissue culture, short period, low cost, and capability of meeting demand of large-area cultivating grease tung, it provides a high effective way for the grease tungartificial breeding, germ-free sprout producing and species of high quality breeding.

The invention provides a simulation test device for a pneumatic load of a vehicle body. The simulation test device is used for a simulation test of the pneumatic load of the vehicle body. The simulation test device consists of a test bed, a fixing mechanism for fixing two ends of the vehicle body to be tested onto the test bed as well as a transverse pressurizing mechanism for applying the pressure on two sides of the vehicle body to be tested, wherein a track for allowing the vehicle body to be tested to move is arranged on the test bed; the transverse pressurizing mechanism comprises a plurality of pressurizing assemblies; each pressurizing assembly consists of an upright post, a wind pressure plate and a hydraulic actuator; the bottom of each upright post is fixedly arranged on the test bed; one end of the hydraulic actuator is hinged with each upright post; and the other end of the hydraulic actuator is hinged with one lateral surface of the wind pressure plate. According to the simulation test device disclosed by the invention, an air pressure load test carried out in the wind pressure plate is replaced by a mechanical device in normal-temperature and normal-pressure environments, and the technical problems that the cost is high and no generality is obtained due to the fact that a pressure container with ultralarge volume is required to be manufactured in the prior art are solved.

The invention discloses an intelligent transformer station secondary equipment state data warning displaying method which comprises the following steps: parsing an SCD file; extracting GOOSE subnet configuration information and MMS subnet configuration information related to warning messages; parsing GOOSE messages and MMS messages matched with the configuration information; extracting APPID, goID, DatSet and the like in the GOOSE messages; extracting DatSet, DataRef and Value in the MMS messages; taking the APPID, goID, DatSet extracted from the GOOSE messages and the DatSet and DataRef extracted from the MMS messages as input for analysis; judging whether the GOOSE messages or the MMS messages are abnormal and require warning or not; conducting grading warning displaying according to a pre-arranged warning grading rule if the GOOSE messages or the MMS messages are abnormal and require warning. According to the intelligent transformer station secondary equipment state data warning displaying method, the warning messages can be graded for displaying, so that on-site operating personnel can visually and accurately know the warning information, and quickly locate and handle failures according to the serious degree of the warning; the operation efficiency is improved; the joint debugging period of a project is shortened.

The invention provides a process for producing semisweet or sweet yellow wine with short production period, high production efficiency, stable quality and unique flavor. The production process can conveniently and alternately generate semisweet and sweet yellow wine as required. The method comprises the following steps of: 1) glutinous rice impregnation; 2) rice steaming; 3) tank filling: filling steamed glutinous rice and purebred raw wheat yeast in an amount which is 5 to 15 percent of the weight of the glutinous rice, block yeast in an amount which is 4 to 5 percent of the weight of the glutinous rice, rice wine in an amount which is 50 to 200 percent of the weight of the glutinous rice and yeast wine in an amount which is 10 to 20 percent of the weight of the glutinous rice into a tank, and starting fermentation at the temperature of between 28 and 30 DEG C; 4) pre-fermentation: raking the materials for the first time in 13 to 16 hours of fermentation, and controlling the fermentation temperature to be below 36 DEG C; 5) wine adding sizing: fermenting for 24 hours, adding the rice wine in an amount which is 300 to 1,300 percent of the weight of the glutinous rice, and stirring uniformly; and 6) post fermentation.

The invention discloses a method for producing phosphocreatine by converting creatine and triphosadenine by a microbial enzyme method. According to the method, a genetic engineering method is adopted; recombinant escherichia coli BL21(DE3)-pET21a(+)-CPK which expresses creatine kinase efficiently is constructed; and the reaction of the creatine and the triphosadenine is catalyzed to produce the phosphocreatine by taking fermentation bacteria or lysate as an enzyme source. The invention provides a new green production process method for the phosphocreatine, which has the advantages of mild conditions, short period, few impurities in an enzymatic system, convenience in separation and extraction of products, simple in process steps, safety in operation and the like; and the method has a very good industrial application prospect.

The invention relates to a preparation method of a graphene/polyimide composite adhesive, which comprises the following steps: adding graphite oxide into an organic solvent, carrying out ultrasonic stripping for 0.5-3 hours to obtain a graphene oxide suspension, adding a silane coupling agent dilute solution into the suspension, and stirring in a 60-80 DEG C water bath for 0.5-1 hour to obtain modified graphene oxide; and adding polyamic acid into the modified graphene oxide, stirring, carrying out ultrasonic blending in an ice bath for 0.5-2 hours to obtain a graphene/polyimide composite material precursor, and curing in a vacuum drying oven at different procedure temperatures. The composite adhesive prepared by the method has the advantages of short period, high speed, high efficiency, simple technique and energy saving, is easy to operate, and can be popularized.

The invention discloses a medlar sterile seedling and a method for obtaining the induced healing of the medlar sterile seedling, belonging to the technical field of plant tissue culture. The method comprises the following steps of drying fruits; disinfecting seeds: carrying out the seed disinfection by using sterile distilled water which contains 70 percent by weight of ethanol and 0.1 percent by weight of TritonX-100 (polyethylene glycol octylphenol ether); germinating seeds: firstly arranging the seeds into a refrigerator at 4 DEG C, standing for 2 days, and then removing the seeds to a culture room at the temperature of 25+/-2 DEG C with the daily photoperiod of 14 hours to culture for 20+/-2 days; healing subculture: arranging the seed on a subculture medium, culturing under at 25+/-2 DEG C in the culture room with the daily photoperiod of 16 hours, and replacing the culture medium every two weeks. According to the method disclosed by the invention, both the medlar sterile seedling and the medlar induced callus are obtained, the step of carrying out physical trauma on the medlar callus in the inducing process of the medlar callus is simplified and the period of obtaining the medlar callus is shortened.

The invention relates to a bulk-shaped hydrophobic flexible silicon dioxide aerogel and a preparation method thereof. The preparation method includes the steps of: 1) mixing trialkoxyl silane containing a vinyl group and mercaptan according to the stoichiometric ratio of an addition reaction, and heating the mixture, adding an initiator, or ultraviolet-irradiating the mixture to prepare trialkoxyl silane containing a C-S bond; 2) mixing the trialkoxyl silane containing the C-S bond with an alcohol solvent according to certain ratio, adding an acidic catalyst with stirring for 1-4 h, adding an alkaline catalyst, uniformly stirring the mixture, and allowing the mixture to stand for 0.1-20 h at 15-90 DEG C for gelatinizing the mixture to prepare silicon dioxide wet gel; and 3) naturally-drying or heat-drying the silicon dioxide wet gel under normal pressure to prepare the bulk-shaped hydrophobic flexible silicon dioxide aerogel. The silicon dioxide aerogel has good bulking performance, is high in porosity, has excellent hydrophobicity and flexibility, is low in heat conductivity, and can be applied in the fields of energy saving for buildings, petroleum chemical engineering, sewage treatment and the like.

The invention discloses a method for preparing nano mullite powder from coal-based kaolin, which comprises the following steps: calcining to activate the raw material coal-based kaolin, and carrying out alkali fusion reaction to prepare the nano mullite powder. Compared with the existing preparation method, the product prepared by the method disclosed by the invention is single-phase nano mullite powder, the raw material is natural mineral which is cheap and accessible, and the invention has the advantages of simple equipment and short period, and is suitable for industrial production.

The invention discloses a process for industrial production of hydrolyzed wheat protein. The hydrolyzed wheat protein is prepared by the following steps of: a, adding water into a reaction kettle, adding alkaline protease into the reaction kettle in a proportion of 20-40U/g wheat gluten protein powder, stirring to be uniform, adding the wheat gluten protein powder into the reaction kettle while alkaline is added to a reaction system to regulate pH value of the system to be 9-10.0, and stopping addition of the alkaline after reaction is carried out for 5-25min; b, when the pH value of the system is 8.5-9.0, adding trypsin into the system, and hydrolyzing for 30-80min at the temperature of 40-65 DEG C, wherein the added amount of the trypsin is 35-60U/g wheat gluten protein powder; c, regulating the pH value to be 7.5-8.0, adding complex enzyme preparation accounting for 0.08-0.5% of the wheat gluten protein powder, and hydrolyzing 1-6h at the temperature of 25-60 DEG C; and d, after hydrolyzing is completed, deactivating enzymatic hydrolysate, then filtering, and finally carrying out spray drying on filtrate. The process for industrial production of the hydrolyzed wheat protein disclosed by the invention has the advantages that protein digestibility is high, the hydrolyzed wheat protein is easy to absorb, the production process is simple and the production cycle is short.

The invention discloses a novel production process of a paraffin wax continuous forming machine, which solves the problems by rationalizing and automating a process flow, shortening a cycle and improving the product quality. The particularly-adopted novel process comprises the following processes: a precooling process, a wax injecting process, a transmitting process, a condensing process, and automatic packaging; wherein the precooling process comprises a warm water closed circulation cooling process, a constant-temperature water closed circulation heat preservation process and a hot air blowing process; the wax injecting process comprises the steps of keeping the precooled liquid wax in a constant temperature and pressure, automatically weighing the wax and injecting the wax into wax discs periodically; the transmitting process comprises the steps of controlling an active power output by a main motor and predicatively controlling an operation state of a main chain; and the condensing process comprises the steps of setting and controlling the area of an evaporator, the evaporating temperature of a refrigerant, the air volume conveyed by an air cooler and the cold energy of a cold chamber. The novel production process of the paraffin wax continuous forming machine has the advantages of: reasonable process flow, optimized parameters, short process cycle, high automation degree, obvious effects of saving energy and reducing emission and high wax injection accuracy.

The invention discloses fertilizer application formulas for different growth stages of garlic. The different fertilizer application formulas for six stages including a planting stage, a seedling stage, a flower bud and bulb differentiation stage, a pedicel elongating stage, a bulbus expansion stage and a quiescent stage are respectively provided according to characteristics of demands of plants to fertilizer in different stages during a garlic growing process, application proportions of azophoska in each stage is controlled, on one hand, the growth of garlic in each stage is promoted, limitation to plant growth caused by less application of a fertilizer can be avoided, and cost increase caused by fertilizer waste due to excess application of a fertilizer can also be prevented, and on the other hand, the garlic grown-up period is shortened, garlic can enter into market in advance and has a stronger superiority of price competitiveness; garlic yield and quality are enhanced due to reasonable fertilizer application and allocation in different stages, the ripening period is shortened, fertilizer cost for the process of garlic planting is reduced, the selling price is higher, and a strong practical signification is achieved.

The invention relates to a method for automatically generating a dual line network by a GIS single line network. The method comprises the following steps: 1) obtaining GIS road data of single line network model, including node layer and road network layer data, and judging the intersection type and road type according to the intersection location and road location; 2) determining the offset direction of the road network node and calculating the offset, forming a road boundary of the dual line network, and generating an offset node of the road network node; 3) performing curve interpolation onthe offset node to indicate the driving path of the vehicle at the road network node, and finally generating a dual line network. Compared with the prior art, the invention has the advantages of low cost, short cycle, and suitable for road maps with low precision requirements.

The invention discloses an auxiliary detection device and a method for an ultraviolet laser interference fringe. In the auxiliary detection device, a visible laser light path is additionally arranged in an ultraviolet laser holographic lithography system; and after the relation between visible laser wavelength and ultraviolet laser wavelength is determined, the period of the ultraviolet laser interference fringe is detected through observing a visible laser interference fringe, therefore, the process of detecting the ultraviolet laser interference fringe in the ultraviolet laser holographic lithography system is simplified, the period of the holographic lithography process is shortened, and the cost of the lithography process is reduced.

The invention discloses an orthogonality determination method for a direct writing system motion platform. The method comprises the following steps: (1) designing a photolithography file, and forming positioning mark points at four corners; (2) fixing a substrate on the motion platform; (3) carrying out direct writing exposure on the substrate according to the photolithography file; (4) recording XY directions, corresponding to the motion platform, of various sides of the substrate and then carrying out developing, cleaning and drying; (5) rotating the substrate 90 degrees and then fixing the substrate on the motion platform of a direct writing system; (6) moving the motion platform, locating the four positioning mark points at the center positions of a CCD respectively, and recording the corresponding position coordinates; and (7) forming a parallelogram by connection lines of the position coordinates and calculating the orthogonality of the motion platform. Compared with the prior art, third-party equipment or tool is not needed, so that the method disclosed by the invention is short in period, simple, convenient, low in cost and suitable for correction of large-width platforms.

The invention discloses a method for efficiently acquiring inductive pluripotent stem cells (iPSCs). Adipose-derived stem cells (ADSCs) are induced and reprogrammed to form the iPSCs by using the method. The method concretely comprises the following steps: (1) inducing cDNA (Complementary Desoxvribose Nucleic Acid) of a multifunctional factor to the ADSCs; (2) culturing the ADSCs by using a serum-free culture medium in a feed-layer-free system, wherein the ADSCs is obtained in the step (1); (3) after cloning sample cells of embryonic stem cells (ESCs), further culturing by using a culture medium with an MEK (Methyl Ethyl Ketone) and GSK3 (Glaxo Smith Klein) signal channel inhibitor, selecting cells to clone, and enlarging culture; and (4) authenticating the cell cloning pluripotency. In the method, the ADSCs are low in cost and easily available on a large scale, and the reprogramming speed and efficiency are very high; the feed-layer-free system can be used for increasing the cloning purity of positive iPSCs; the serum-free culture medium can be used for avoiding unstable factors brought by undefined components in serum and batch difference; the signal channel inhibitor can be used for promoting cells to be up to the sufficient reprogramming state and improving the cloning quality of the positive iPSCs.

The invention discloses a method for preparing a nano mesoporous carbon microsphere-graphene sandwich composite material, graphene oxide is dissolved in a solvent A to obtain a graphene oxide dispersion; a structure directing agent is dissolved in a solution B, then a carbon source is added for full reaction to obtain a mesoporous carbon precursor mixed liquid; the graphene oxide dispersion is added into the mesoporous carbon precursor mixed liquid for reaction to obtain a complex; and the complex is successively aged and carbonized. The nano mesoporous carbon microsphere-graphene sandwich composite material with a high specific surface area, uniform morphology and a controllable pore structure can be prepared by the method at low temperature, according to actual needs, the amount of the structure directing agent and the carbon source can be adjusted for control of the pore structure. Equipment needed in the method is simple, and the method is short in cycle, simple in process and low in cost.

The invention provides a method for manufacturing a low-melting-point alloy mold. The method comprises the following steps of: (1) designing a computer aided design (CAD) model according to a material object, and manufacturing a laminated object prototype; (2) mixing and stirring silicon rubber components according to a ratio, and manufacturing a silicon-rubber mold in a vacuum injection molding machine to serve as an intermediate conversion mold; (3) weighing gypsum mixture and water according to a ratio, and manufacturing a gypsum mold core on gypsum type vacuum grouting equipment; (4) manufacturing an upper ceramic mold and a lower ceramic mold; and (5) closing the mold and pouring, pouring alloy melt, solidifying and cooling the alloy to obtain the alloy mold. The indirect manufacturing method is suitable for manufacturing the low-melting-point metal mold which is complex in shape, fine in surface pattern and high in precision requirement and has wide application prospect and market prospect.

The invention provides a method and apparatus for realizing an MTTCP in an intelligent terminal device. The method comprises the following steps: obtaining an Android operating system source code used by the intelligent terminal device, and transplanting the source code in a VirtualBox virtual machine, so that tan Android operating system can run in the VirtualBox virtual machine; running the Android operating system integrated with the MPTCP on the VirtualBox virtual machine, and constructing a multipath transmission control protocol MPTCP network environment to realize the function test of the MPTCP; and after the test is successful, copying an MPTCP source code file to the Android operating system source code used by the intelligent terminal device, and performing a test on the intelligent terminal device. By adoption of the method of integrating the MPTCP code in the Android kernel, the correctly tested MPTCP code in the VirtualBox virtual machine environment can be directly applied to the intelligent terminal device adopting an Android platform, thereby shortening the period of applying the multipath transmission control protocol to the actual network environment, and accelerating the popularization and application progress of the MPTCP in the mobile internet can be greatly improved.

The invention relates to a brewing technology of strawberry wine and the strawberry wine prepared through the brewing technology. The brewing technology includes the steps that 1, strawberry syrup is prepared; 2, the sugar degree of the strawberry syrup is adjusted at the earlier stage; 3, active dry yeast is treated; 4, the strawberry syrup is subjected to natural fermentation; 5, strawberry fermentation stock is subjected to low-temperature temperature control fermentation; 6, strawberry base wine is subjected to stability treatment; 7, blending of the strawberry base wine and aftertreatment of the strawberry wine are performed. The brewing technology has the advantages that the period of the technological treatment process is shortened as far as possible so that the loss of the strawberry flavor can be reduced; natural substances are used for fermentation, the two-stage fermentation technology with natural fermentation and low-temperature temperature control fermentation combined is adopted, neither additives nor industrial components are used in the whole process, the original nutritional ingredients, fruity aroma and color and luster of strawberries are maintained, and the wine has a mellow aroma; the strawberry wine is more outstanding in color, aroma and taste due to the low-temperature temperature control fermentation technology and is good in stability after later-stage blending treatment.

The invention discloses a cellular array chip based on adjustable micromagnetic field, belonging to the technical field of biomedical engineering. The chip is formed by sequentially laminating a substrate layer, a grounding layer, a first insulating layer, a microcoil array layer, a second insulating layer, a ferromagnet layer and a third insulating layer, wherein a conducting window is respectively etched on the first insulating layer, second insulating layer, ferromagnet layer and third insulating layer; the microcoil array layer is composed of a microcoil array and a positive pole bonding pad; and the ferromagnet layer is provided with a through hole array corresponding to microcoils on the microcoil array layer. In the chip disclosed by the invention, the patterned microcoil array is utilized to form a gradient magnetic field and some columns of intangible magnetic particle cages; and when the magnetic particles are attracted to the center, the magnetic particles entering the inside of the magnetic field can not depart from the restriction of the magnetic field if not having sufficient power, thereby enhancing the cell patterning efficiency, shortening the cell patterning period and simplifying the unicell patterning operation steps.

The invention relates to a method of quickly biodegrading a lignocellulose source inhibiting substance. The method particularly includes the steps of: 1) pre-treating a lignocellulose raw material; 2) quickly biodegrading the lignocellulose raw material in a reactor to remove the compounds which are generated during the pretreatment and have inhibition effects on subsequent enzymolysis and fermentation; and 3) performing the enzymolysis and subsequent processes to the material treated in the step 2). The method reduces treatment period to 6-36 h on the basis of ensuring biodegrading efficiency on the inhibiting substances, so that efficiency and practicability of the process are greatly improved. The biodegrading process is amplified into reactor level and is integrated with a subsequent conversion process in the same reactor, so that the integration degree of a bio-refining process is increased. The method lays the foundation of application of biodegradation of the inhibiting substances in the lignocellulose bio-refining process.

The invention discloses a method for establishing a hyperlipidaemia rat model in a short term. Animals are male Sprague Dawley rats, the body weight of the rats is 150+/-20 grams. A high-fat diet formula includes 23.31% of casein, 0.35% of L-cystine, 8.48% of corn starch, 11.65% of maltodextrin, 20.17% of saccharose, 5.83% of cellulose, 2.91% of soybean oil, 20.68% of lard oil, 5.23% of compound mineral substances, 1.16% of compound vitamin, and 0.23% of Choline bitartrate. A fat emulsion formula includes 20% of Tween 80, 20% of lard oil, 10% of cholesterol, 2% of sodium cholate, 20% of propylene glycol, 20% of glucose, and 1% of propylthiouracil. The operation method includes: constinuously feeding rats with the high-fat diet for 21 days, and performing self-feeding; feeding the rats with the fat emulsion prepared according to the formula through the gavage method, wherein the dosage is 2 ml/kg. day. The method is short in establishment cycle of the rat hyperlipidaemia animal model, is low in cost, is good in reproducibility, can effectively simulate the human incidence state. Accordingly, the method can be used for research on functional foods capable of preventing hyperlipidaemia and screening of lipid-lowering drugs.

The invention discloses a site contaminated soil distribution determination method based on geological conditions. The method includes the following steps: 1) collecting the geological parameters of a to-be-tested site, comprising: soil geologic stratification, soil layer permeability, soil granular size, soil organic substance content, and water level, flow direction and flow speed of underground water, and determining the background values of the parameters; 2) collecting the environmental data of the site to determine the types of the pollutants; 3) determining investigation depth according to the types of the pollutants and the soil layer permeability coefficient; and 4) determining investigation zones according to the soil layer permeability coefficient, the soil organic substance content, and the water level, the flow direction and the flow speed of underground water. The method, on the basis of the character analysis of geological conditions, can be used for macroscopically determining and investigating the characters of distribution and migration of local soil pollutants with combination of the environmental data through various test methods. The method is low in workload, is short in period and is low in cost. A diagnosis result can effectively guide arrangement of sampling points and determination of sampling depth in site environment investigation in later period.