1608 results about "Gene knockout" patented technology

The present invention relates to the fields of genetic engineering and plant genetic breeding, and specifically to the application of wheat TaPND gene. In the present invention, by taking gene TaPND as a target, designing a sgRNA sequence based on CRISPR / Cas9, connecting a DNA fragment containing the encoding sgRNA sequence to a vector carrying CRISPR / Cas, a plant binary expression vector capable of knocking out the TaPND gene in wheat is constructed; by genetically transforming wheat, offspring with TaPND gene knockout are obtained, and wheat mutants with early flowering time are obtained. Experiments have confirmed that transgenic wheat with TaPND gene knockout achieves the advancement of wheat flowering time. The technical scheme of the present invention provides a practical method for realizing rapid wheat breeding using genetic engineering technology, which has important breeding application value and broad market application prospects.

The invention discloses a wheat salt-tolerant gene and application thereof. Specifically, the invention discloses a protein TaSGR (SEQ ID NO: 3, 6 and / or 9) and an application of a coding gene of the protein TaSGR in regulation and control of plant salt tolerance and / or plant height. According to the invention, a homozygous gene editing plant of which the TaSGR gene is knocked out is successfully created by utilizing a CRISPR / Cas9 technology. The result shows that after the TaSGR gene is knocked out, the plant height of the wheat is obviously reduced, and the salt tolerance is obviously enhanced. The invention discloses the application of the protein TaSGR and the coding gene thereof for the first time. The salt tolerance of the plant is increased and / or the plant height of the plant is reduced by reducing the content and / or activity of the protein TaSGR (for example, the TaSGR gene is knocked out or silenced). According to the invention, a good gene resource is provided for salt-tolerant breeding of wheat, a new field is developed for application of the TaSGR gene, and the TaSGR gene has important significance for cultivation of novel salt-tolerant wheat.

The invention relates to the field of rice gene engineering, and particularly provides OsSULTR2; the invention also discloses application of the 2 protein and the coding gene thereof in regulating and controlling the salt tolerance of rice. The protein meets the following conditions: B1) a protein with an amino acid sequence of SEQ ID NO.1; and B2) a fusion protein with the same function obtained by connecting a tag to the N end and / or C end of B1). The method is used for detecting OsSULTR2; the salt stress phenotype identification in the seedling stage is carried out on the transgenic rice with the gene knockout 1, 2, and the result shows that when the gene segment is deleted, the salt stress tolerance of the rice is improved, and the function and the application way of the gene are proved. Therefore, the OsSULTR2 of the present invention; the 2 protein and the coding gene thereof can regulate and control the salt tolerance of rice, and have important significance for cultivating salt-tolerant transgenic rice.

The invention discloses a porcine muscle stem cell line capable of being cultured in vitro for a long time and application of the porcine muscle stem cell line, and belongs to the technical field of stem cells and cell culture meat. The porcine muscle stem cell line is prepared by the following steps: performing CDKN2A gene knockout on porcine muscle stem cells by using a CRISPR / Cas9 gene editing technology, performing monoclonal screening and genotype identification to obtain a cell strain with successful CDKN2A knockout, and then performing carrier-free and serum-free suspension culture domestication on the cell strain. And a carrier-free and serum-free porcine muscle stem cell line capable of being subjected to in-vitro long-term suspension culture is obtained. Verification shows that the CDKN2A knockout porcine muscle stem cell line subjected to carrier-free and serum-free suspension culture domestication not only can be subjected to carrier-free and serum-free suspension culture in a shake flask for a long time, but also can maintain a good differentiation characteristic. The porcine muscle stem cell line which can be cultured on a large scale, is derived from edible animals and can be cultured in vitro for a long time is created, and excellent cell resources are provided for commercial production of cultured meat.

The invention belongs to the technical field of plant molecular biology, and particularly relates to an oilseed rape flowering negative regulation transcription factor BnMYB615, a cloning method, a BnMYB615 gene overexpression vector, a BnMYB615 gene knockout vector, a host cell, a flowering phase delay transgenic plant and application of a flowering phase advance gene editing plant. A nucleotide sequence of the rape transcription factor BnMYB615 is shown as SEQ ID NO.1, an amino acid sequence of the rape transcription factor BnMYB615 is shown as SEQ ID NO.2, an overexpression vector sequence of the rape transcription factor BnMYB615 is shown as SEQ ID NO.3, and a gene knockout vector sequence of the rape transcription factor BnMYB615 is shown as SEQ ID NO.4. The overexpressed BnMYB615 can obviously delay the flowering time of plants, and can prolong the growth season and the photosynthetic time of the plants and obtain higher yield when being applied to crops taking nutritive organs as harvest objects; after the BnMYB615 gene is knocked out, the flowering time of the plant is advanced, and when the BnMYB615 gene is applied to crops taking seeds or fruits as harvest objects, the growth period of the crops can be shortened, the multiple cropping index can be improved, meanwhile, the influence of adverse climate can be avoided, and the product quality can be improved.

The invention discloses regulation and control of a GmERECTAs gene (GmERs for short) on a soybean plant height and application of the GmERECTAs gene (GmERs for short). The invention belongs to the technical field of biology. An sgRNA sequence of a GmERs gene is recombined to a knockout vector pGES401 through a Golden Gate cloring technology. According to the method, four homologous genes of GmERs are knocked out by using a CRISPR / Cas9 technology, the four genes comprise GmERa, GmERb, GmERc and GmERd, and offspring screening is performed through herbicide resistance endowed by a Bar marker gene; after a positive result is obtained through screening, the GmERs gene knockout condition is determined through sequencing, and the plant height of a gene knockout soybean plant is remarkably reduced compared with that of a contrast. The invention has important significance for developing and breeding new soybean germplasm resources.

The invention discloses an application of an HERP gene knockout mouse model in obesity and metabolic disorder diseases. The invention finds that compared with a wild type high-fat group, HERP gene knockout can cause phenotypes such as early obesity, more serious obesity degree, increase of fat weight ratio, aggravation of adipose tissue inflammation, dyslipidemia and glucose metabolism disorder of mice during high-fat feeding. And the HERP gene is knocked out, so that the adipocyte differentiation is increased. The HERP gene knockout mouse is more sensitive to nutrition, obesity can occur more quickly during high-fat feeding, symptoms such as fat amplification, fat inflammation and glucose and lipid metabolism disorder are more serious, and the HERP gene knockout mouse can be used for molecular mechanism research and drug screening of obesity, fat tissue and glucose and lipid metabolism disorder. It is further found that cilostazol can significantly relieve mouse obesity and lipid metabolism disorder caused by HERP gene defects, but has no obvious weight losing effect on normal genotype mice, and a new strategy is provided for precise treatment.

The invention discloses a cotton GhSNL1 protein as well as a coding gene and application thereof. The cotton GhSNL1 protein coding gene (GhA01G1757; according to the present invention, the CRISPR-Cas9 gene editing material of the GhSNL1 gene is subjected to phenotypic analysis to discover that the GhSNL1 gene knockout material has the phenotype of fiber start slowing, fiber density shortening and mature plant boll regeneration, such that the GhSNL1 gene participates in the cotton fiber development process, and the cotton fiber development efficiency is significantly improved; the method provides a theoretical basis for cotton variety improvement, and can be used for cultivating new cotton varieties with excellent fiber quality and high yield.

The invention belongs to the technical field of molecular biology, and particularly relates to sphingomonas paucimobilis recombinant engineering bacteria as well as a construction method and application thereof. The engineering strain is obtained by knocking out a metabolic bypass gene from sphingomonas paucimobilis, and the metabolic bypass gene is composed of a crtI gene or a phaC gene. The production amount of gellan gum can be increased by the delta phaC knockout strain and reaches 8.9 g / L. In addition, overexpression of energy metabolism related genes pgi or clpB or ycaJ is carried out, and the yield of a strain with the overexpressed pgi gene is increased to the maximum and reaches 9.5 g / L. Therefore, the pgi gene is overexpressed on the basis of the delta phaC knockout strain, the yield of gellan gum is further increased to 10.0 g / L, and the production efficiency is improved.

The invention relates to the field of microbial genetic transformation, and discloses a preparation method of Ilyonectria fungus protoplast and a construction method of a genetic transformation system.The preparation method comprises the following steps that S11, Ilyonectria fungus hyphae are prepared, Ilyonectria fungi are cultured on a PDA solid culture medium for 14 d, then the Ilyonectria fungi are inoculated to a CMC liquid culture medium to be subjected to shake-flask culture for 4 d and filtered, filtrate is centrifuged for 10 min at the speed of 10000 rpm, and spores are collected; diluting the concentration of spores to 107 pieces / mL, inoculating the spores into a YEPD liquid culture medium, carrying out shake-flask culture for 2 days, and filtering and collecting hyphae; and S12, preparing the Ilyonectria fungus protoplast, namely adding the hypha prepared in the step S11 into the enzymatic hydrolysate, performing enzymolysis in a shaker at the temperature of 30 DEG C at the rotating speed of 150-200rpm for 3-5 hours, filtering, centrifuging filtrate at the temperature of 4 DEG C, discarding supernate, adding 0.7 M NaCl for centrifuging, and discarding the supernate, thereby obtaining the Ilyonectria fungus protoplast. The genetic transformation constructed by the invention can be used for exploring the specific action and mechanism of a specific pathogenic gene in the pathogenic process of Ilyonectria fungi through gene knockout or overexpression experiments.

The invention relates to the technical field of microbial metabolism engineering, in particular to a construction method and application of genetically engineered bacteria for efficiently producing O-acetyl-L-homoserine. The construction method specifically comprises the following steps: (1) taking a strain Escherichia coli W3110 as a chassis, and knocking out a homoserine O-succinyl transferase coding gene metA; (2) knocking out a gamma-cystathionine synthetase encoding gene metB; (3) knocking out a cysteine lyase coding gene metC; (4) carrying out weakening expression on an O-acetyl-L-homoserine thiolase coding gene metYcm from the marine cyclobacterium; (5) overexpressing a ribulose diphosphate carboxylase coding gene rbcLSse from the synechococcus elongate; (6) overexpressing a ribulose phosphate kinase coding gene prkse from the synechococcus slender; and (7) weakening the expression of the citrate synthase coding gene gltA to obtain the genetically engineered bacterium for efficiently producing the O-acetyl-L-homoserine. The strain provided by the invention is used for producing O-acetyl-L-homoserine, and has the advantages of high yield, high yield and high sugar-acid conversion rate.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a wheat magnesium transporter TaNIPA8-6B and application of a coding gene thereof to regulation and control of plant stripe rust resistance. Functional analysis is carried out on the wheat magnesium transporter TaNIPA8-6B by means of gene editing and overexpression, and it is found that the wheat magnesium transporter TaNIPA8-6B induces expression in both affinity and non-affinity system reactions of wheat and puccinia striiformis, so that a mutant plant of the gene editing and overexpression TaNIPA8-6B gene is created; according to the TaNIPA8-6B gene, the gene knockout mutant of the TaNIPA8-6B gene is identified to show susceptibility to stripe rust non-compatible races CYR23, the over-expression mutant plant shows disease resistance to stripe rust compatible races CYR32, the fact that the TaNIPA8-6B gene plays a positive regulation role in the disease resistance of wheat to stripe rust is clear, and a high-quality germplasm resource is provided for stripe rust resistance of wheat.

The invention relates to a gene editing vector of agrobacterium, a gene editing method of the gene editing vector and an agrobacterium editing strain. The agrobacterium gene editing vector comprises the following gene elements: a plasmid skeleton, a Pvan promoter, a spCas9 gene, a green fluorescent protein gene GFP, gRNA, and an upstream homologous arm and a downstream homologous arm of a target gene. According to the agrobacterium gene editing vector, a CRISPR / spCas9-GFP gene editing system is adopted, an efficient, simple, convenient and traceless genome editing system is constructed in agrobacterium, and gene knockout and insertion can be achieved.

The invention relates to an L-valine production strain as well as a construction method and application thereof, the strain is obtained by transformation through a metabolic synthesis path of L-valine, and the construction method comprises the following steps: by taking Cornebacterium glutamicum ATCC 13032 (C.glutamicum) as an original strain, carrying out editing transformation such as gene knockout, gene overexpression, gene heterologous expression, gene double-copy expression and the like on a genome of the original strain by utilizing a metabolic engineering means. The L-valine production strain disclosed by the invention can be used for synthesizing L-valine from the beginning by taking glucose as a carbon source, has the advantages of high production rate, short fermentation period, high strain stability and low cost, and lays a foundation for realizing large-scale production of L-valine. According to the fermentation tank fermentation method, the L-valine is produced in a mechanical stirring type fermentation tank by using the L-valine production strain Va1-09, 86.4 g / L of L-valine is produced by fermenting for 36 hours, and the method has a good industrial prospect.

The invention belongs to the technical field of biology, and particularly relates to an SLC44A1 gene and / or SLC44A5 gene knockout animal model as well as a construction method and application thereof. The construction method of the animal model comprises the following steps: deleting exon regions from the fourth exon region to the thirteenth exon region of the SLC44A1 gene and / or the second exon region of the SLC44A5 gene in mouse brain oligodendroglia cells in a high-efficiency manner by utilizing gene editing and a Cre-loxP recombinase system, so as to realize the knockout of a target gene. The animal model constructed by the invention can be used for researching the regulation mechanism of the choline transporters (SLC44A1 and SLC44A5) on myelin sheath formation, deeply knowing the biological functions of the choline transporters (SLC44A1 and SLC44A5), simulating the occurrence and development processes of human related diseases, revealing the pathogenesis of the related diseases, providing a new thought for prevention and treatment of brain myelin sheath dysplasia, and providing a new foundation for the prevention and treatment of brain myelin sheath dysplasia. And an effective drug or a treatment mode aiming at the disease is screened by utilizing a gene knockout animal model, so that a new choice is provided for clinical treatment of the disease.

The invention belongs to the technical field of gene engineering, and particularly discloses a construction method and application of a toxoplasma gondii gene knockout vaccine strain, a TGGT1202920 gene of toxoplasma gondii is directly knocked out through a gene editing technology to obtain a delta TGGT1202920 strain, and the strain has the characteristics that the strain can be normally cultured in vitro and is almost non-toxic in vivo; after animals are immunized with delta TGGT1202920 strains, good immune protection can be generated, infection of wild type toxoplasma gondii can be resisted, and the strain can be used for preparing toxoplasmosis resistant vaccines to prevent toxoplasma gondii infection.

The invention relates to a recombinant pichia pastoris genetically engineered bacterium for expressing recombinant full-chain-length humanized collagen as well as a preparation method and application of the recombinant pichia pastoris genetically engineered bacterium, and belongs to the technical field of genetic engineering. The recombinant pichia pastoris genetically engineered bacterium disclosed by the invention expresses recombinant full-chain-length humanized collagen, and a ku70 gene of the recombinant pichia pastoris genetically engineered bacterium is knocked out and knocked into a recombinant 4-proline hydroxylase encoding gene. Recombinant full-chain-length humanized collagen is optimized, a 4-proline hydroxylase gene and a collagen gene are co-expressed in pichia pastoris to obtain hydroxylation modified recombinant collagen, and a corresponding recombinant pichia pastoris genetically engineered bacterium is provided, has proteolytic enzyme A defect and hydroxylation modification capability, and can be used for preparing a recombinant collagen product. The stability of the collagen in the fermentation process is improved. The recombinant full-chain-length humanized collagen provided by the invention is 100% homologous with III-type collagen derived from a human body, so that the immune risk is extremely low, and the biological activity is more perfect.

The invention relates to an anti-cryptosporidium micromolecular lead compound of a target cryptosporidium helicase. Comprising the following steps: S1, detecting enzyme activity parameters of a drug target helicase (CpeIF4A) and an inhibition effect of Roc-A on the drug target helicase: expressing and purifying a CpeIF4A recombinant protein containing an MBP tag; detecting the enzyme activity; detecting the inhibition effect of the Roc-A on the enzyme activity; determining the affinity between the Roc-A and the CpeIF4A through a heat transfer experiment; s2, in-vitro drug effect determination: evaluating the direct killing capability of Roc-A on sporozoites; the inhibition effect of Roc-A on cryptosporidium is determined through an in-vitro cell infection model, an EC50 value is obtained, and the intracellular period of the Roc-A mainly playing the inhibition effect is determined; detecting the toxicity of Roc-A to host cells to obtain a TC5 value), and obtaining an in-vitro insect-resistant selectivity index (SI); and S3, in-vivo drug effect test: establishing a C57BL / 6IFN-gamma gene knockout mouse infection model to evaluate the effectiveness and safety of Roc-A. The invention determines that the Roc-A has the potential of being further developed into the cryptosporidium-resistant drug, and verifies that the targeted eIF4A can resist cryptosporidium infection.

The invention relates to the technical field of grass carp (Ctenopharyngodon idella), in particular to a method for knocking out a grass carp SMYD3 gene and application of the grass carp SMYD3 gene. The nucleotide sequence of the gRNA is as shown in SEQ ID NO: 1. The method comprises the following steps: synthesizing gRNA of a target gene through in-vitro transcription, then mixing the gRNA with Cas9 protein, introducing the mixture into an animal pole in a grass carp fertilized egg single-cell period by adopting a microinjection mode, and hatching to obtain the grass carp F0 generation with the target gene mutation. According to the application, the knockout of the grass carp gene is efficiently realized, and an effective technical means is provided for developing grass carp breeding and gene function research.

The invention belongs to the technical field of gene engineering, and relates to improvement of drought resistance of cotton, in particular to application and a method of a transcription factor GhTCP67 in improvement of the drought resistance of the cotton. According to the application, the transcription factor GhTCP67 is used as a drought negative regulation gene in cotton, and the plant height can be inhibited by knocking out the gene, but the drought resistance of the cotton can be enhanced. Therefore, the GhTCP67 can be used as a candidate gene for cultivating a cotton drought-resistant variety, and the gene is mutated from a genome level by utilizing a gene editing technology, so that a new cotton genetic germplasm with reduced plant height and enhanced drought resistance is obtained. According to the application, a new research idea is provided for cotton drought resistance research, and meanwhile, a cotton genetic material with enhanced drought resistance is also created. The invention further provides a recombinant plasmid constructed by using the DNA fragment and an obtained gene editing transgenic plant and seed.

The invention provides a wheat drought-tolerant gene TaTZF1 and application thereof in plant drought tolerance, and belongs to the technical field of gene engineering. The invention provides a wheat drought-enduring gene TaTZF1. The amino acid sequence of a protein encoded by the wheat drought-enduring gene TaTZF1 is as shown in SEQ ID No. 2. The wheat drought-enduring gene TaTZF1 is subjected to induced expression by drought, ABA and salt stress, the wheat drought-enduring gene TaTZF1 is overexpressed and transformed into wheat in an overexpression mode, and it is found that the drought tolerance of a transgenic plant with overexpressed TaTZF1 is remarkably improved compared with that of a wild type plant; after the wheat drought-tolerant gene TaTZF1 in wheat is knocked out, the drought tolerance is obviously reduced. It is proved that the wheat drought-resistant gene TaTZF1 positively regulates the drought resistance of wheat, gene resources are provided for wheat molecular breeding, and the wheat drought-resistant gene TaTZF1 has great significance in improvement of wheat drought-resistant genetic improvement.

Disclosed herein are an engineered immune cell with CD7 gene knock-out and use thereof. According to the present invention, an sgRNA specifically targeting CD7 gene is designed and synthesized, which can accurately target CD7 gene to achieve gene knock-out with high knock-out efficiency. The provided sgRNA can be used for preparing a CD7-targeting engineered immune cell, and can be further used for preparing a CD7-targeting universal CAR-T cell

The invention relates to the technical field of biology, in particular to a recombinant strain for producing nicotinamide mononucleotide and application of the recombinant strain. According to the method, accumulation of the NMN is improved through metabolic flux redirection and gene knockout, a synthesis path of the NMN is successfully established in escherichia coli, and further, the yield of the NMN is effectively improved and reaches 7.0 g / L by optimizing a transport system of NAM-NMN and a PRPP synthesis path. The invention also optimizes the production process of the NMN, realizes the highest yield of the NMN of 28.5 g / L in a fed-batch fermentation system, and obviously improves the yield compared with the traditional batch fermentation.

The invention discloses a pichia pastoris engineering bacterium for synthesizing bakuchiol from de novo by converting methanol as well as construction and application of the pichia pastoris engineering bacterium. A heterologous p-coumaric acid synthesis pathway is introduced to neutral sites of different chromosomes of pichia pastoris, a high-yield p-coumaric acid strain is obtained through gene knockout, overexpression or heterologous expression of tyrosine and phenylalanine synthesis pathway key genes of a shikimic acid pathway, a heterologous bakuchiol biosynthesis pathway is introduced on the basis, and a high-yield p-coumaric acid strain is obtained. After overexpression of bakuchiol synthetase, endogenous overexpression or heterologous expression of MVA pathway key genes and exogenous acetyl coenzyme A supply pathway genes, methanol concentration optimization and bacterial strain His4 gene supplementation, the yield of bakuchiol is effectively increased to 91.2 mg / L and is increased by 59.8 times compared with that of an initial bacterial strain, and the yield of bakuchiol in a 15L fermentation tank reaches 692.8 mg / L. The method has the characteristics of high conversion efficiency, low production cost, convenience in preparation, wide industrial application prospect and the like.

The invention discloses application of LECT2 as a diagnosis and treatment target of hepatic ischemia reperfusion injury. According to the present invention, the Print method operation patient sample is detected to discover that the expression of the LECT2 in the hepatic ischemia reperfusion injury is up-regulated; on the basis, a liver ischemia reperfusion injury model is constructed by knocking out a mouse through the LECT2 gene, and a result shows that the liver ischemia reperfusion injury of the mouse is obviously reduced by knocking out the LECT2 gene and transferring the LECT2-shRNA into the LECT2 gene, and the liver ischemia reperfusion injury of the mouse is aggravated by LECT2 recombinant protein; it is shown that inhibition of expression of LECT2 is beneficial to improvement or treatment of hepatic ischemia injury; furthermore, the invention further finds that liver injury can be relieved by inhibiting the combination of LECT2 and Tie1; the LECT2 / Tie1 signal channel can also be used as a target spot for treating the liver ischemia reperfusion injury.

The invention discloses application of a rice transcription factor OsWRKY39 and a coding gene thereof in rice blast resistance of rice, and belongs to the technical field of gene engineering. According to the invention, an overexpression vector and a gene knockout vector of the rice OsWRKY39 gene are constructed, the vector is transferred into a Kitaake receptor material, an OsWRKY39 overexpression plant and an OsWRKY39 knockout plant are obtained, and the obtained transgenic plants are subjected to disease resistance analysis. Results show that knockout of the OsWRKY39 gene significantly improves the resistance level of rice to rice blast, which indicates that the OsWRKY39 negatively regulates the disease resistance of plants, and the encoding gene OsWRKY39 can be used as a target gene for improving the disease resistance of plants, has a good application prospect in cultivation of disease-resistant rice varieties, and lays an important foundation for rice resistance breeding.

The invention belongs to a molecular biological technology, discloses a construction method of a uterus stromal specific gene editing mouse, and relates to a CRISPR-Cas9-based stromal specific gene editing mouse. The Cre mouse using Vim as a specific promoter activates Cas9, and the Cas9 mouse not only solves the problem of complex breeding of the traditional Cre mouse, but also can realize tissue specific knockout without homozygosis. Cas9 mice can be used for knocking out various sgRNAs, and more possibilities are provided for development of a gene editing model. The obtained Cas9 mouse is mated with an sgMettl3 report mouse, and the Mettl3 gene is successfully knocked out in the uterine stromal cells in a guided manner. The invention provides a valuable gene editing model for endometrial gene research, and overcomes the challenge of difficult knockout of bispecific site mediated genes.

The invention belongs to the field of application of microbial genetic engineering and metabolic engineering, and particularly relates to a method for improving the biosynthesis efficiency of sclareol. Specifically, in a host strain, gene knockout is realized through a CRISPR / Cas9 technology, or / and a DNA fragment is introduced, and a strain metabolic pathway is adjusted to obtain a corresponding engineering strain; carrying out biosynthesis by utilizing the obtained engineering strain to obtain sclareol; the gene knockout is realized by introducing donor DNA (Deoxyribose Nucleic Acid) containing upstream and downstream homologous arms of a coding frame; the DNA fragment sequentially comprises an upstream homologous arm, a promoter, a gene, a terminator and a downstream homologous arm from upstream to downstream, the host strain is selected from any one of saccharomyces cerevisiae. According to the method, the cell life is associated with product synthesis, and meanwhile, metabolic balance is coupled, so that the synthesis efficiency of a target product is improved, and a foundation is laid for industrial fed-batch fermentation.

The invention discloses a method for inducing sugarcane to resist sugarcane smut, and belongs to the technical field of sugarcane disease resistance. The method disclosed by the invention comprises the following steps: (1) obtaining sugarcane smut Pep1 gene knockout mutants PepMAT-1 and PepMAT-2; (2) culture of a sugarcane smut Pep1 gene knockout mutant: activating PepMAT-1 and PepMAT-2, and then inoculating the activated PepMAT-1 and PepMAT-2 to a YePS liquid culture medium for shake cultivation; and (3) inducing sugarcane to resist smut: after shake cultivation, mixing the PepMAT-1 and the PepMAT-2, and inoculating the mixture on sugarcane seedlings. The method can rapidly and efficiently induce sugarcane to resist sugarcane smut, and provides technical support for prevention and treatment of sugarcane smut.

The invention belongs to the technical field of aquatic organism breeding, and discloses a method for creating a novel grass carp germplasm capable of resisting infection of grass carp hemorrhagic disease virus (grass carp reovirus type II GCRV-II) based on a smd3 gene editing technology and application of the novel grass carp germplasm. Gene editing is carried out on wild grass carp by taking the smd3 gene as a target gene, an F0-generation chimera with the smd3 gene knocked out is selected to carry out a GCRV-II challenge experiment, and the experiment result shows that the smd3 knocked-out chimera of the grass carp has high GCRV-II virus infection resistance, so that a method is provided for genetic improvement of virus infection resistance of economic fishes such as the grass carp and the like. The method has important industrial application value.