1665 results about "High flux" patented technology

The invention belongs to the technical field of data transmission and processing, and discloses a zero-copy-based multi-thread high-throughput data stream channel separation method and system, and the method comprises the steps: directly writing a mixed data stream into a front-end buffer region configured as an annular structure through a data receiving module by adopting direct memory access; then, a multi-thread processing module dynamically allocates a plurality of processing threads from a thread pool to separate channel data in parallel, each thread adopts a zero copy algorithm based on pointer offset, positions the channel data in a memory, creates pointer reference and associates the channel data to a corresponding rear-end buffer area, and logic separation is achieved without physical copy; and finally, the data storage module efficiently writes the separated data into persistent storage in an asynchronous I / O mode. According to the method, zero-copy, multi-thread parallel and two-stage dynamic buffering strategies are combined, the data separation efficiency is remarkably improved, CPU occupation and memory bandwidth are greatly reduced, and the real-time performance and stability of high-throughput data processing are guaranteed.

The invention relates to a poplar property high-throughput accurate quantitative identification method, system, equipment and medium, the innovation of the method lies in that spectral data analysis and automatic script processing are combined, efficient data acquisition, import, matching and storage are realized, abnormal point detection is carried out by introducing principal component analysis and anti-noise mahalanobis distance, a data set is optimized, and the identification accuracy of the poplar property is improved. Building and training a model to realize high-flux accurate prediction of the material property and the density; a sample control module, an acquisition test module, an automatic processing module, a data set determination module and a result output module are integrated on the system, so that full-process automatic and intelligent management is realized; the equipment comprises a database and a memory, and the method is cooperatively executed through the database and the memory to ensure efficient operation of the method; a computer readable medium stores executable instructions, which is convenient for wide deployment and application. The method is excellent in high-throughput analysis, cost saving, environmental protection and data accuracy, and development of the poplar property identification technology is effectively promoted.

The invention relates to the technical field of metabolite detection, in particular to a high-flux metabolite detection method and application thereof. According to the detection method, metabolites in a biological sample to be detected are detected by using a liquid chromatography-mass spectrometry technology, wherein mass spectrometry is quadrupole electrostatic field orbitrap high-resolution and triple quadrupole mass spectrometry; the mass spectrum conditions of the MRM ion pair are as follows: acquiring data in an NFSWI-DDA, NFSWI-DIA or DIA mode in a positive and negative ion mode, and acquiring and annotating the MRM ion pair; the MRM list is transited to triple quadrupole mass spectrometry, and the mass spectrometry conditions of the triple quadrupole mass spectrometry include verification and detection of MRM ion pairs in positive and negative ion modes. The detection method can meet the requirements of precise medical research on metabolite coverage rate, quantitative stability and detection flux, can be used for semi-quantitative and quantitative combined detection of metabolites in various biological samples, has applicability, and can provide technical support for disease diagnosis, drug development and biomarker discovery.

The invention discloses a high-flux testing device for accurately testing the dynamic erosion corrosion performance of a material, which comprises a testing kettle, a rotating system, a liquid circulating system and a liquid collecting tank, the rotating system comprises a motor and a rotating blade, and the rotating blade is provided with a sample; the invention further discloses a testing method for high-flux accurate testing of the dynamic erosion corrosion performance of the material. The method comprises the step of injecting the corrosion solution to scour a sample. The rotation system is arranged to realize rotation of the sample, the test kettle and the liquid circulation system matched with the test kettle are arranged to realize erosion of the corrosion solution to the sample, the flow rate is controllable, the corrosion solution can be reused, and a plurality of samples can be subjected to long-time erosion corrosion test in the same environment and under different test conditions. The corrosion performance of the material under the same corrosion environment, different operation states and different temperatures is accurately obtained, so that the function of accurately testing the dynamic erosion corrosion performance at high flux is realized, and the testing device has the characteristics of high safety coefficient, convenience in operation and the like.

The invention belongs to the field of astronomical telescopes, and particularly relates to a large-aperture spliced telescope adjusting device and method, and the method comprises the steps: starting a light source module, providing a far-field illumination light beam for a spliced primary mirror, and carrying out the confocal adjustment of each sub-mirror through an adjusting mechanism according to the far-field illumination light beam; the light source switching module is used for providing a near-field illumination light beam for the spliced primary mirror, and the near-field illumination light beam is split into two separated sub light beams by the pyramid mirror after penetrating through a splicing seam of the spliced primary mirror; performing dispersion on all the sub-beams through a dispersion prism, and converging the dispersed sub-beams through an achromatic lens to form dispersion stripes; the phase difference of the dispersion fringes is extracted through the camera, and co-phase adjustment is carried out on the sub-mirrors through the adjusting mechanism according to the phase difference of the dispersion fringes. According to the invention, high-precision and high-flux spectral response calibration and co-phase detection of the large-aperture spliced telescope can be realized, so that reliable technical support is provided for construction and operation of a next-generation giant astronomical telescope.

The invention discloses a preparation method and device of a low molecular weight cutoff ultrafiltration membrane, and belongs to the technical field of ultrafiltration membrane preparation. A thermally induced phase separation method and a non-solvent induced phase separation method are combined to prepare the membrane, the relationship between the flux and the retention rate of the ultrafiltration membrane is balanced, high flux is considered while high retention of low-molecular-weight substances is provided, an upper-layer membrane casting solution and a lower-layer membrane casting solution are used for double-layer simultaneous coating, a substrate is coated with the membrane casting solutions, and the membrane is obtained through a dry process section. Phase separation is carried out after entering a coagulating bath, a finished membrane is obtained through cleaning, the obtained membrane is of an integrated structure, the coagulating bath used in the membrane casting process is pure water, and recycling and aftertreatment of the ultrafiltration membrane are facilitated.

An x-ray source includes an x-ray transmissive window having an x-ray transmittance greater than or equal to 20% for at least some x-rays having an x-ray energy less than 1 keV. The x-ray source further includes an electron source configured to generate at least one electron beam and an anode assembly configured to generate x-rays in response to electron bombardment by at least some of the electrons of the at least one electron beam from the electron source. The x-ray source further includes at least one x-ray optic is configured to receive at least some of the x-rays from the anode assembly and to direct at least some of the received x-rays to the window to form an x-ray beam.

The invention relates to an eVTOL (electric vertical take-off and landing aircraft)-oriented multipath transmission optimization and dynamic path selection method, and aims to solve the problems of bandwidth, delay and packet loss of an eVTOL aircraft in high-throughput video data transmission. According to the method, key performance indexes such as bandwidth, delay and packet loss rate of a path are monitored in real time, and path selection is optimized in combination with flight state data, so that low-delay and high-bandwidth transmission of a panoramic video stream is ensured. An improved multi-path load balancing strategy is adopted, the flow is distributed according to the path score and the bandwidth margin, network congestion is avoided, and the data transmission stability is improved. By enhancing an MPQUIC protocol and optimizing multi-path cooperative transmission, a low-delay path is preferentially selected to transmit high-priority data. A redundant transmission mechanism ensures that data can still be reliably transmitted through other paths even if a part of paths lose packets. The method provides technical support for the construction of an immersive eVTOL driving experience platform, meets the requirements of low time delay, high bandwidth and high sense of reality, and has a wide application prospect.

The invention provides an immersed laser direct-writing photoetching method and device. The immersed laser direct-writing photoetching device comprises a laser light source, a laser beam splitter, an acousto-optic modulator, a dichroscope-spectroscope module, an acousto-optic deflector, an objective lens, an immersion head and a motion platform, the motion platform is used for placing a wafer to be photoetched; the laser light source, the laser beam splitter, the acousto-optic modulator and the dichroscope-spectroscope module are sequentially arranged along a first straight line, the dichroscope-spectroscope module is arranged in a deflection mode relative to the first straight line, and the acousto-optic deflector, the objective lens, the immersion head and the motion platform are sequentially arranged along a second straight line; laser generated by the laser light source sequentially passes through the laser beam splitter and the acousto-optic modulator and is emitted to the dichroscope-spectroscope module, the dichroscope-spectroscope module selectively reflects laser with specific wavelength, and the reflected laser sequentially passes through the acousto-optic deflector, the objective lens and the immersion head and is emitted to a wafer to be photoetched. And high-speed and high-flux parallel laser scanning direct writing photoetching can be realized.

The utility model provides a high-flux mold for spark plasma sintering and sintering equipment thereof, and relates to the technical field of sintering, the high-flux mold comprises at least one pressure head and a porous mold; the multi-hole die comprises one or two hole opening faces, and a plurality of closed holes are formed in the hole opening faces. The closed holes are formed in the vertical direction of the porous mold, the hole diameters of the multiple closed holes are the same, and the closed holes are used for containing sintering raw materials; a plurality of sub-pressure heads are arranged on the pressure head, and the sub-pressure heads are in one-to-one correspondence with the plurality of closed holes; and the pressure head comprises a large pressure head. By adopting the high-throughput mold provided by the embodiment of the utility model, samples can be prepared on a large scale through one-time spark plasma sintering, so that the efficiency and sustainability of a spark plasma sintering process are improved, and the component screening and optimization of the samples are further accelerated.

The invention relates to a high-flux selective diffusion agent and application thereof. The high-flux selective diffusion agent is obtained by mixing heavy rare earth-containing spherical powder and a solid organic binder and then melting and processing; the particle size of the heavy rare earth-containing spherical powder is 10-60 [mu] m, and the sphericity is greater than 85%. According to the method, the heavy rare earth-containing spherical powder with high sphericity degree and good fluidity and the raw material suitable for additive manufacturing are adopted, and the comprehensive magnetic performance of the subsequently prepared magnet can be remarkably improved by strictly controlling the morphology of the heavy rare earth-containing spherical powder. According to the method, the high-flux selected area dispersion technology is combined with the additive manufacturing means, selected area diffusion is conducted on the surface of the neodymium-iron-boron magnet, and the coercive force and high-temperature stability of the neodymium-iron-boron magnet can be greatly improved under the condition that the use amount of heavy rare earth is small. The method is suitable for multi-batch and small-batch production exploration and has the advantages of being high in technological process automation process and short in research and development period.

The invention discloses a plant root system image and moisture high-flux in-situ automatic scanning system, and relates to the field of plant root system detection, the system comprises a transparent pipeline buried in soil and a pipeline robot moving in the transparent pipeline; the pipeline robot comprises a chassis, a lifting platform and a data acquisition unit; the chassis is used for driving the lifting platform and the data acquisition unit to move in the transparent pipeline; the lifting platform is used for driving the data acquisition unit to move up and down; the data acquisition unit is arranged on the lifting platform and used for acquiring root system images of plants outside the transparent pipeline and moisture information of soil where the plants are located. The system can automatically monitor the growth and development conditions of the profile root system and the corresponding moisture information according to the manually set measurement time interval, realizes the lossless, in-situ and high-throughput observation of the profile root system, reduces the consumption of manpower and material resources, and ensures the integrity of the plant root system.

The invention discloses a water quality comprehensive toxicity high-throughput determination method, which comprises the following steps: performing exposure contamination on a tested organism by using a sewage sample to obtain phenotypic characteristic data of the tested organism; constructing a toxicity matrix; establishing a machine learning model, and determining the water quality comprehensive toxicity of the sewage sample in combination with the toxicity matrix. The water quality comprehensive toxicity determination method provided by the invention does not need sample concentration and enrichment, ensures the comprehensiveness and accuracy of the detection result, and can be applied to the comprehensive toxicity high-flux determination of the sewage in the whole process of a sewage treatment plant.

The invention discloses a fast thermal neutron and gamma ray multi-mode detector, and relates to the technical field of nuclear radiation detection, and the detector comprises a detector housing, and the interior of the detector housing is connected with a fast neutron detection unit, a thermal neutron detection unit and a gamma ray detection unit. The gamma ray detection unit is located between the fast neutron detection unit and the thermal neutron detection unit; and an insulating separation unit is connected between two adjacent detection units. The detector can detect fast neutrons, thermal neutrons and gamma rays in the same equipment at the same time, structures such as a cadmium thermal neutron shielding layer and a fast neutron moderation layer do not need to be added, the size of the detector can be greatly reduced, and the detector has the advantages of being compact in structure, stable in performance, wide in energy measurement range, wide in fluence rate measurement range and the like. The ultrahigh-energy high-flux neutron measurement with the neutron energy at least covering # imgabs0 # and the neutron fluence rate at least covering # imgabs1 # can be realized.

The invention belongs to the technical field of optical fiber communication, and relates to an eight-mode mode division multiplexer based on a multi-core optical fiber, which comprises a substrate material and a central few-mode fiber core, a high-refractive-index ring is arranged in the central few-mode fiber core; the peripheral single-mode fiber core comprises a first single-mode fiber core used for carrying out LP11a mode conversion on the central few-mode fiber core, a second single-mode fiber core used for carrying out LP31 mode conversion on the central few-mode fiber core, a third single-mode fiber core used for carrying out LP11b mode conversion on the central few-mode fiber core, and a fourth single-mode fiber core used for carrying out LP21b mode conversion on the central few-mode fiber core; the fifth single-mode fiber core is used for carrying out LP12 mode conversion on the central few-mode fiber core; the sixth single-mode fiber core is used for carrying out LP21a mode conversion on the central few-mode fiber core; and the seventh single-mode fiber core is used for carrying out LP02 mode conversion on the central few-mode fiber core. The invention has the characteristics of large number of conversion modes, high space utilization rate, low insertion loss, high flux and the like.

Holographic volume gratings in waveguide cells can be recorded using many different methods and systems in accordance with various embodiments of the invention. One embodiment includes a holographic recording system including at least one laser source configured to emit recording beams and a movable platform configured to move between a first position and a second position, wherein when the movable platform is in the first position, the at least one laser source is configured to emit a first set of one or more recording beams toward a first set of one or more stations and when the movable platform is in the second position, the at least one laser source is configured to emit a second set of one or more recording beams toward a second set of one or more stations.

The invention discloses a method for accurately deducting Sr double-charge interference in in-situ Ca isotope determination, and relates to the technical field of isotope analysis. According to the method, the Sr double-charge interference in the in-situ Ca isotope measurement is accurately deducted through an iteration method, the Ca isotope measurement result can be simply and quickly corrected, and high-resolution, low-detection-limit and high-flux Ca isotope measurement is realized; ca single-charge fractionation factors are calculated through an iterative method, and instrument quality discrimination correction and accurate deduction of Sr double-charge interference are simultaneously realized through formulas 1-5 in combination with the Sr isotope ratio; the method breaks through the application limitation of in-situ Ca isotope measurement, is suitable for high-Sr and low-Sr samples, solves the technical problems of in-situ Ca isotope analysis accuracy and analysis precision, fills the technical blank of Ca isotope measurement in the Sr-rich sample in laser in-situ detection, and provides a firm technical support for application in the field of geoscience.

The utility model relates to the technical field of optical detection, and discloses a turntable high-flux Raman testing device which comprises a base, a detector is fixedly connected to one side of the top of the base, a Raman probe is fixedly connected to one side in the detector, a first motor is fixedly connected to one side in the base, and a second motor is fixedly connected to one side in the base. And the output end of the motor I is fixedly connected with a solid double-layer rotating disc. According to the utility model, the motor I drives the rotating disc to rotate, so that the transparent sample bottles are moved to rotate along the rotating disc, and the transparent sample bottles pass through the Raman probes one by one for chemical component analysis. The rotation of the turntable is controlled through a control program, and the probe is controlled to collect Raman signals of samples, so that full-automatic component analysis of multiple samples is realized. The problem that a measurement result is inaccurate due to the fact that a probe needs to be moved due to the fact that the surface of liquid is uneven when a perforated plate is used for collecting a sample for testing is solved, and the measurement accuracy and efficiency are improved.

The invention discloses a high-throughput multi-row parallel high-speed scanning high-resolution microscopic imaging device and method based on adaptive optics. A spatial light modulator is used for carrying out pixel-level regulation and control on a light beam phase to generate a strip-shaped light spot, and a special arrangement mode of a detector array is used for realizing a one-to-many relationship between a scanning line and an imaging line and realizing improvement of an imaging speed, a signal-to-noise ratio of an imaging result and a resolution ratio; a full-digital area array wavefront regulation and control method is utilized, a spatial light modulator is used for rapidly and dynamically compensating a laser wave surface according to imaging result feedback, the laser wave surface is free of distortion, and the influence of aberration on an imaging result is reduced; designing a double telecentric Fourier transform structure capable of simultaneously keeping rotational symmetry of object plane and image plane light beams, and realizing distortionless mapping of scanning light beam quality and a linear signal system; a switchable design of two scanning modes is developed, high-flux, high-resolution and high-speed light field scanning is realized, and the advantages of large visual field, high resolution and high frame rate imaging are achieved.

The invention discloses a DLD chip and method based on high-flow-speed composite flow channel multi-target sorting. The chip comprises a sample inlet, a sheath fluid inlet, a rectification module and a two-stage deterministic lateral displacement DLD sorting module, the rectification module adopts a circular microcolumn array to optimize fluid distribution, and the first-stage module realizes primary sorting through a microfluid channel and is connected with the second-stage module; and the second-stage module is communicated with three sample outlets after being accurately sorted through a microfluid channel II. The chip is made of a PDMS material through a soft photoetching process, and the two stages of sorting modules are designed to be of a rectangular structure connected end to end and are respectively composed of elliptical micro-columns with opposite deflection angles. Under the high-flow-rate working condition, stable separation of cells can be kept, blockage is not prone to occurring, the good sorting effect on various cells of different sizes is achieved, the flow channel layout is compact, the chip manufacturing process is simplified, and the chip is suitable for the sorting scene with the high-flux requirement.

The invention relates to the technical field of microfluidics, in particular to a high-throughput and high-efficiency cell extrusion microfluidic system and a particle introduction method. And a closed-loop control system integrating real-time cell deformation sensing, dynamic parameter decision and physical extrusion and alternating electric field cooperative application is constructed. The chip is provided with an inlet shunting device, a micro-channel and a channel structure of a microelectrode. The method comprises the steps of real-time deformation image acquisition and analysis and dynamic extrusion parameter decision and cooperative application. The invention provides a high-flux and high-efficiency cell extrusion microfluidic system and a particle introduction method, and aims to solve the problems that the drug loading efficiency and the survival rate are difficult to consider due to heterogeneity in the existing cell extrusion technology and a physical extrusion and electroporation coordinated regulation strategy is lacked, so that personalized precise treatment of cells is realized, and the cell quality is improved. The drug loading efficiency, the cell survival rate and the high throughput are obviously improved.

According to the efficient water treatment system based on the reverse osmosis membrane and the control method provided by the invention, gradient anti-pollution membrane arrangement and reconfigurable intelligent waterway design are creatively combined, so that a foundation is laid for high-flux and low-pollution operation from a physical structure; meanwhile, a full-closed-loop intelligent control system is constructed, and by fusing fuzzy logic, an optimization algorithm and a neural network prediction model, the system can integrate multi-dimensional information such as inlet water quality, membrane pressure difference and water yield, judge pollution risks in real time and adaptively and seamlessly switch among a high recovery mode, a segmented flushing mode and a prediction cleaning mode, so that the system is high in recovery efficiency and high in reliability. Meanwhile, parameters such as pump frequency and valves are dynamically optimized to find an optimal balance point of recovery rate and energy consumption; according to the invention, the recovery rate can be stably improved, the membrane pollution rate is greatly reduced, the chemical cleaning period and the service life of the membrane element are prolonged, and finally, the comprehensive operation and maintenance cost of the system is remarkably reduced while the water treatment efficiency and the water quality safety are improved.

The invention discloses a high-heat-flux heat dissipation system for an on-orbit computing power cluster, and belongs to the technical field of spacecraft heat control. The system is independent of a satellite conventional thermal control platform and is specially used for discharging waste heat of an AI calculation array. The system comprises a heat capture unit, a pumpless heat transfer unit and a foldable heat dissipation unit. The heat transmission unit comprises a micro-channel flexible pulsating heat pipe (such as a diamond film material), and the heat pipe spans a folding area and has the functions of structural connection and heat transmission, so that a thermal low-impedance independent channel relative to a satellite structure is formed. Through the design of removing a mechanical pump and removing a mechanical hinge, the problems of large weight, micro-vibration interference, high contact thermal resistance and the like of a heat dissipation system of the computing power satellite are solved, and preferential directional heat dissipation of high-flux heat is realized.

The invention discloses a monitoring and lifting integrated device for a high-throughput biological culture system. The monitoring and lifting integrated device comprises a central cantilever, a rotating platform mechanism and a lifting mechanism, wherein the central cantilever is coaxially connected with the rotating platform mechanism in a rotating manner; the lifting mechanism is arranged at one end of the central cantilever and used for lifting the pore plate to move in the Z-axis direction; the monitoring mechanism is arranged at the other end of the central cantilever and is used for illuminating and observing the cell state of the pore plate. Compared with an existing cell or organoid culture and analysis mainstream scheme, the system has the advantages that the integration degree is higher, the observation and analysis speed is higher, strict environmental conditions can be kept in the culture and analysis process and the like, the brand-new requirements of a high-flux biological culture system under the industrial and automatic background can be met, and the application prospect is wide. Technical and theoretical possibilities are provided for successful cell or organoid culture and observation of an intelligent automatic production line.

The invention relates to the technical field of biological tissue engineering, and discloses a man-machine interaction type automatic manufacturing system for organoid or other biological tissues, the man-machine interaction type automatic manufacturing system comprises a workbench, a mechanical arm, an industrial camera and a control module, a first covered stent of an array manufacturing area on the workbench is matched with a supporting assembly to form a containing cavity, and inert gel is arranged in the containing cavity; pressing the inert gel into a concave hole array by using an array mold to form a first cell carrier; the cell inoculation area is provided with a dehydration platform and a dehydration module, and the first covered stent is detachably connected with the dehydration module; the industrial camera is used for acquiring a concave hole array position image; the control module controls the mechanical arm to drive the pipette to implant cells into the concave hole array; a second cell carrier is arranged in the second covered stent, and the second cell carrier enters the concave hole array and wraps the cells to form a cell trap array. According to the human-computer interaction automatic manufacturing system for the organoid or other biological tissues, the high-flux manufacturing process of the organoid can be simplified, and the controllability and the consistency of the regeneration process of the organoid are improved.

The invention relates to the technical field of semiconductor packaging and cooling, and particularly discloses a diamond-copper composite micro-channel-based cooling and packaging structure which comprises a packaging shell and a diamond-copper heat conduction plate with the bottom in floating lap joint through an elastic sealing ring. A plurality of heat exchange stand columns are integrally formed on the upper surface of the heat conduction plate, and the heat exchange stand columns are inserted into the containing counter bores of the packaging shell to form micro-channel annular gaps. A piezoelectric vibration plate dividing the medium cavity into a storage cavity and a jet flow cavity is integrated in the packaging shell, and the jet flow cavity communicates with a flexible connecting pipe section arranged over the heat exchange stand column in a suspended mode. Spiral fins are arranged on the surfaces of the heat exchange stand columns, horn-shaped flow guide pieces are arranged at orifices of the containing counter bores, and C-shaped groove flow guide fins which are arranged in a staggered mode are arranged on the bottom face of the heat conduction plate. According to the invention, low-thermal-resistance, high-flux and high-reliability direct contact type active heat dissipation for a high-power-density device is realized.

The invention provides a high-flux multi-scale integrated non-adhesive flexible riser mechanical property simulation method, which comprises the following steps: establishing RVE of riser models with different layers based on ABAQUS, and applying complex periodic boundary conditions by using MPC to obtain a hysteresis loop of a riser; a self-proposing elastic-plastic constitutive model is used, an FORTRAN language is used for writing a running program, and by adjusting parameters of the constitutive model, the response of the elastic-plastic constitutive model is made to be consistent with a hysteresis loop of a riser RVE; establishing a UEL suitable for the ABAQUS according to the elastic-plastic constitutive model, establishing a large-scale homogenized riser model by using the UEL, and performing analysis and research; and the function of researching the mechanical response of the large-scale non-adhesive flexible riser under stretching, bending, torsion and internal and external pressure loads is realized. According to the method, the mechanical behaviors of the non-cohesive riser model under the complex working conditions are more comprehensively understood, and more accurate guidance is provided for engineering design, material selection and performance optimization.

The invention provides a method for detecting an external vesicle marker through high-flux nano plasma exciting light immune color development, and belongs to the technical field of human extracellular vesicles. After a serum sample is subjected to centrifugal treatment, the serum sample and a CD81 capture antibody substrate are incubated, and the particle size distribution of the vesicles is monitored in real time; a zwitterionic polymer modified gold nanoparticle LAM detection probe and a polyethylene glycol modified silver nanoparticle LprG detection probe are prepared to be specifically combined with a vesicle surface antigen, a chromogenic enhancement solution is adopted to induce a plasma resonance signal, and full-hole scanning imaging is carried out; and constructing a double-layer game optimization model to cooperatively optimize the detection sensitivity and the signal stability, carrying out weighted summation on normalized signals of the particle size subgroups to obtain comprehensive detection signal intensity, comparing the comprehensive detection signal intensity with a threshold value, and outputting a final judgment result. The technical problem that quantitative accuracy is affected by signal intensity deviation caused by vesicle particle size difference in outer vesicle marker detection is solved.

The invention provides a continuous flow magnetic separation chip and a preparation method thereof.The chip comprises an upper pressing plate, a lower pressing plate, a chip bottom plate and a flow channel cover plate, the flow channel cover plate is arranged on the chip bottom plate in an attached mode, a flow channel is formed between the flow channel cover plate and the chip bottom plate, and the flow channel cover plate is further provided with a flow channel inlet and a flow channel outlet; the chip bottom plate and the runner cover plate are fixedly clamped between the upper pressing plate and the lower pressing plate, soft magnetic strip materials are packaged in the chip bottom plate and the runner cover plate, and the soft magnetic strip materials are located on one side of the runner. The soft magnetic strip material patterned array is used, and the soft magnetic strip materials are packaged in the chip bottom plate and the flow channel cover plate, so that double-layer arrangement is formed in the vertical direction, magnetic force distribution with the inner side direction of the flow channel as the main direction and the longitudinal direction as the auxiliary direction is achieved, the cell wall attaching effect is effectively weakened, high-flux cell magnetic control screening can be achieved, and the cell magnetic control screening efficiency is improved. And the magnetic field screening efficiency is improved. In addition, during cell sorting, gradient grading regulation and control can be achieved in the aspect of magnetic field force.

The invention provides a high-throughput multi-view automatic positioning light sheet microscopic imaging method. The method comprises the following steps: automatically identifying the size of a sample, presetting parameters, and carrying out preliminary scanning; splicing imaging data in real time; globally and automatically positioning a sample area; planning a shooting path; and multi-target area high-throughput shooting parameters are optimized. A microscope large-view high-flux rapid imaging method based on a real-time image automatic splicing and positioning technology is combined with a real-time splicing function of shot imaging data and an automatic positioning function of a sample position in an image to rapidly search specific position area information of a sample; and shooting imaging and data storage of the experimental sample are realized by automatically optimizing shooting configuration parameters. The invention further provides a high-throughput multi-view automatic positioning light sheet microscopic imaging system adopting the method, and shooting imaging and data processing can be rapidly and accurately carried out on a target large sample.