539 results about "Highly sensitive" patented technology

To improve allele discrimination, the inventors adapted the system combining Cas13a detection sensitivity with allele-specific PCR amplification to propose CASPER (Cas13a Allele-Specific PCR Enzyme Recognition) as a new versatile, easy-to-implement, and highly sensitive method to detect low-frequency of sequence variant. CASPER enabled specific and sensitive detection of KRASG12D with low DNA input such as DNA extracted from patient's pancreatic ultrasound-guided fine-needle aspiration fluids. CASPER is easy to implement and a versatile reliable method virtually adaptable to any point mutation.

The invention discloses a divalent nucleic acid aptamer fluorescence detection method based on an exonuclease I auxiliary target circulation strategy, and belongs to the field of analysis and detection.The divalent nucleic acid aptamer is a repetitive sequence composed of two monovalent nucleic acid aptamers and can form double-stranded DNA through base complementary pairing with complementary strand cDNA of the divalent nucleic acid aptamer, and the divalent nucleic acid aptamer can be used for fluorescence detection of the divalent nucleic acid aptamer. The method comprises the following steps: taking a bivalent nucleic acid aptamer as a template, forming a DNA silver nanocluster (DNA-AgNCs) under the action of silver nitrate and sodium borohydride, and when the target okadaic acid exists, combining the bivalent nucleic acid aptamer with the target, so that cDNA can be replaced from double-stranded DNA by the target. Exonuclease I is added to perform enzyme digestion on the cDNA and the divalent nucleic acid aptamer, so that the fluorescence intensity of the DNA-AgNCs can be rapidly reduced. The content of the okadaic acid can be judged by detecting the change of the fluorescence intensity. The invention provides a simple, convenient, rapid, high-sensitivity and high-specificity detection method for okadaic acid detection based on a divalent nucleic acid aptamer for the first time.

The invention discloses an alginate-based high-entropy metal oxide as well as a preparation method and application thereof, and belongs to the technical field of gas sensor materials. The preparation method comprises the following steps: mixing a high-entropy metal salt solution of at least five metal sources with an alginate solution to prepare alginate-based high-entropy metal aerogel, and then wrapping the alginate-based high-entropy metal aerogel with carbon cloth and rapidly heating the alginate-based high-entropy metal aerogel by using a Joule thermal method to obtain the nanosheet-shaped alginate-based high-entropy metal oxide. The material can be used for high-sensitivity detection of ethanol gas. The preparation method provided by the invention is green, simple and rapid, has a good sensing response effect in ethanol gas detection, and the sensing material has good stability, can be recycled for multiple times, and has a good application prospect.

The invention discloses a composition, a detection method and a kit for SpaceAS variable splicing space in-situ detection of nucleic acid. The composition comprises a pi-type probe, a U-type probe, an O-type probe, an S-type probe and a signal probe, and the O-type probe adopts an in-vitro cyclization strategy, so that the problem of low hybridization efficiency caused by dependence on enzymatic cyclization reaction in a traditional hybridization method is effectively solved; meanwhile, an efficient probe hybridization system, a high-sensitivity signal amplification mechanism and a multi-round iterative imaging process are combined, so that the detection sensitivity and specificity are remarkably improved. The detection method has the advantages of high efficiency, high sensitivity, high specificity, good repeatability, strong expandability and the like through the synergistic effect of five-step single-stranded DNA probe hybridization reaction and one-step DNA amplification reaction, and can be widely applied to space in-situ detection and analysis of variable splicing events.

The invention relates to the technical field of environment control, and discloses a time-sharing optimization control method for a cold storage central air conditioner based on a large model, which comprises the following steps of: firstly, acquiring a system cold demand quantity and an environment working condition predicted value at a future moment through a pre-trained deep learning model, and estimating a current cold storage charging and discharging state in combination with a thermal front degradation correction algorithm; then, a non-linear flow resistance model associated with the porosity and the cold storage state is established, and a hydraulic high-energy-consumption interval in which fluid resistance is highly sensitive to flow change is recognized; and on the basis, deducing the energy efficiency trend of the water chilling unit by utilizing the environment temperature to determine load distribution, actively inhibiting cold storage output in a high flow resistance interval through energy efficiency gating logic, and finally generating a time sequence control strategy containing flow and load instructions to realize optimal scheduling of system-level energy efficiency.

This invention provides a CRISPR / Cas12a fluorescence detection method for FocTR4 based on magnetic bead-ssDNA-HRP probes, belonging to the field of nucleic acid detection technology. This invention involves isothermal amplification of the FocTR4 target sequence using RPA primers; then, a magnetic bead-ssDNA-HRP probe is prepared; and the Cas12a / crRNA complex, RPA amplification product, and probe are co-incubated. The activated Cas12a cleaves the probe, releasing HRP. After magnetic separation, a colorimetric solution containing OPD is added, and the presence and concentration of FocTR4 are determined by detecting the fluorescence signal. This invention combines the high efficiency of CRISPR / Cas12a cleavage with the high sensitivity and strong specificity of RPA technology, achieving highly sensitive and specific detection of FocTR4, with a linear detection range of 10. 2 ~10 7 With a detection limit as low as 70 CFU / mL, it can quickly and accurately detect Foc TR4 in soil samples, enabling rapid on-site detection and providing reliable technical support for the early diagnosis and control of banana wilt disease.

This invention belongs to the field of gas sensor technology and discloses a method for preparing europium ferrite nanosensors and their applications. This method utilizes electrospinning technology combined with a specific low-temperature confined-domain calcination process (550℃~700℃) to prepare EuFeO3 nanotubes with a hollow structure and rough surface by taking advantage of the thermal decomposition kinetics differences of poly(p-v) magnets (PVP). This process effectively suppresses excessive repair of lattice oxygen while forming a unique tubular morphology, effectively "freezing" oxygen vacancies (O2) accounting for up to 34.44% on the material surface in situ. v This invention addresses the shortcomings of traditional solid fiber materials by constructing a sensor using a grinding, dispersion, and coating process. Utilizing the dual reactive surfaces of hollow nanotubes and high-concentration oxygen vacancy active sites, it achieves highly sensitive and selective detection of low-concentration ethylene gas released during fruit ripening. This invention solves the problems of small specific surface area and insufficient active sites in traditional solid fiber materials.

This invention discloses a near-infrared long-afterglow nanocomposite, its preparation, and its application in uric acid detection, belonging to the technical field of testing or analyzing materials by measuring their chemical or physical properties. The nanocomposite comprises a near-infrared long-afterglow luminescent center and an outer layer of organosilicon-doped mesoporous silicon containing tetrasulfide bonds. Under near-infrared light excitation, this nanocomposite exhibits excellent afterglow luminescence enhancement response to hydrogen peroxide. Since the product of uric acid decomposition by uricase is hydrogen peroxide, this composite enables highly sensitive and rapid detection of uric acid in blood. The composite provided by this invention has uniform particle size and high stability, effectively eliminating autofluorescence interference from biological components and improving detection accuracy.

This invention discloses a method for fabricating a highly sensitive long-wavelength infrared superconducting single-photon detector. It fully utilizes and amplifies the acoustic mismatch effect between the superconducting thin film and the substrate to achieve phonon localization enhancement on the superconducting nanowires. The effect of this innovative design is to increase the energy utilization rate of long-wavelength infrared photons on the superconducting nanowires, thereby improving the response sensitivity of the superconducting nanowires to long-wavelength infrared photon signals. Compared to existing designs that reduce the cross-sectional size of the superconducting nanowires, create holes beneath the nanowires, or increase the buffer layer, this invention is easier to implement and has higher scalability.

This application discloses a Mycoplasma pneumoniae P1 protein-binding protein, its preparation method, and its uses. The Mycoplasma pneumoniae P1 protein-binding protein includes an immunoglobulin single variable domain, wherein the immunoglobulin single variable domain comprises CDR1, CDR2, and CDR3 contained in VHH as shown in any one of SEQ ID NO: 1-8. The bivalent or multivalent nanobodies prepared using this Mycoplasma pneumoniae P1 protein-binding protein can effectively enhance their binding strength to antigens, providing a superior raw material for developing highly sensitive detection and diagnostic applications.

This invention relates to the field of chemiluminescence immunoassay technology, specifically to a magnetic bead coating method and its application for improving the luminescence level of p-tau 217. This invention optimizes the process of coating p-tau 217 capture antibodies with carboxyl magnetic beads. By gradient adjustment of the input ratio of EDC and NHS, the optimal activator concentration is obtained to enhance the magnetic bead coupling activity, increase the antibody coupling efficiency, and thus improve the signal values ​​of antigen samples and clinical samples. This invention has broad application prospects for the development of highly sensitive detection of low-abundance proteins such as p-tau 217.

This invention relates to a body fluid-based detection device for chronic diseases, which belongs to the field of medical devices. The device includes a body fluid detection fluid channel, a detection probe, a data processing module, and a data display module. The detection probe is disposed within the body fluid detection fluid channel, and the detection probe is connected to the data processing module, which is connected to the data display module. The body fluid detection fluid channels include: a body fluid ion detection channel, a body fluid pH detection channel, a body fluid hydrodynamic characteristic detection channel, a channel for detecting the content of soluble gases such as oxygen, a channel for detecting the physical characteristics of white blood cells and tumor cells, a channel for detecting the electrical characteristics of white blood cells and tumor cells, a channel for detecting the physical characteristics of red blood cells, a channel for detecting the electrical characteristics of red blood cells, a channel for detecting the electrical characteristics of separated body fluids, and a channel for detecting anticoagulation and fibrinolysis functions. This invention enables highly sensitive, non-invasive testing, providing a powerful tool for the early diagnosis of chronic diseases.

This invention discloses a homogeneous microsphere based on singlet oxygen self-circulating aggregation-induced emission, its preparation, and a wash-free detection method, which improves the sensitivity and efficiency of wash-free luminescent oxygen channel immunoassay. This invention successfully prepared AIE receptor microspheres by embedding the photosensitizer AIER dye, ultra-bright AIEG dye, and singlet oxygen-responsive dye SO into carboxyl-modified polystyrene nanoparticles. Simultaneously, an antibody-1-labeled AIE receptor microsphere solution, an antigen-containing test solution, and biotinylated antibody-2 were added to a 96-well plate, followed by streptavidin-modified donor microspheres. After wash-free incubation, the fluorescence signal value was measured, and the concentration of antigen in the test solution was obtained according to a standard curve. This platform completes wash-free, highly sensitive detection within 5 minutes, innovatively combining the aggregation-induced emission characteristics of AIEgens and a singlet oxygen cycling strategy, providing an efficient and universal solution for wash-free diagnostics.

A LiGa-based 0.5 In 0.5 A self-driven X-ray detector based on Se2 crystal and its fabrication method. This detector employs an MSM device structure with c-plane LiGa... 0.5 In 0.5 Using Se2 wafers as semiconductor materials and metal materials as electrodes, self-driven X-ray detection can be achieved under zero bias voltage; the above-mentioned self-driven X-ray detector fabrication method includes the following steps: (1) fabricating LiGa 0.5 In 0.5 (1) Se2 crystal; (2) Directional processing of c-plane LiGa 0.5 In 0.5 Se2 wafer; (3) LiGa 0.5 In 0.5 The upper and lower surfaces of the Se2 wafer are polished and passivated; (4) in LiGa 0.5 In 0.5 Metal electrodes are deposited on the upper and lower surfaces of a Se2 wafer. This invention utilizes LiGa... 0.5 In 0.5 The bulk photovoltaic effect generated by the non-centrosymmetric structure of Se2 enabled the fabrication of a self-driven X-ray detector with a simple structure. Furthermore, due to the LiGa... 0.5 In 0.5 Se2 crystals have high resistivity, and the devices fabricated from them have low dark current, enabling highly sensitive X-ray detection.

Method for manufacturing a flexible strain sensor, comprising: Placing a flexible substrate on a movable stage in a working chamber, in which the movable stage, suitable for a desired movement according to a control signal, and a nozzle, suitable for spraying towards an upper surface of the movable stage, are installed, and with which a first pressure control unit, suitable for controlling an internal pressure of the working chamber, is combined; and preparing a printing material mixture, which comprises metal nanoparticles and carbon nanotubes (CNTs) in a powder form, which is to be introduced into a printing material tank, which is provided with an upper outlet that communicates with a nozzle via a first communication line, and a lower inlet, with which a second pressure control unit, suitable for controlling pressure, is combined. Control, by means of a control unit, a movement of the movable stage by providing a predetermined motion control signal to the movable stage in order to move the flexible substrate at a desired speed along a path which corresponds to a predetermined conductor pattern of a strain sensor, Forming a relatively low-pressure atmosphere within the working chamber by operating the first pressure control unit and simultaneously a relatively high-pressure atmosphere at a lower inlet of the pressure material tank by operating the second pressure control unit, In parallel with controlling the movement of the movable stage, the printing material mixture is forcibly transmitted in an aerosolized state through the first communication line from the printing material tank to be sprayed through the nozzle towards a surface of the flexible substrate by means of a compression wave, which is caused by a pressure difference between the low-pressure atmosphere and the high-pressure atmosphere. Direct printing of a predetermined conductor pattern of the strain sensor onto the flexible substrate by a process in which the printing material mixture, which is extruded through the nozzle, collides with the surface of the flexible substrate to create cracks on the surface, and the CNTs penetrate into the cracks and are mechanically anchored to the flexible substrate, and then the following metal nanoparticles and CNTs of the printing material mixture are deposited in a predetermined width and height on the surface of the flexible substrate. Connecting a first and a second lead wire, which extend electrically to protrude from the flexible substrate, to both ends of the predetermined conductor pattern of the strain sensor and Binding a flexible cover, which has the same size as the flexible substrate, to the surface of the flexible substrate on which the predetermined conductor pattern is printed, so that the predetermined conductor pattern is inserted between the flexible substrate and the flexible cover.

The invention relates to the technical field of virus detection, and discloses a method and a kit for detecting Chinese sacbrood virus. The method comprises: firstly, carrying out isothermal amplification on a CSBV specific target sequence in a sample nucleic acid by using an RPA technology, the RPA technology adopting a specific primer pair, the sequences of which are SEQ ID NO: 4 and SEQ ID NO: 5; then, an amplification product is mixed with a CRISPR / Cas12b detection system, and the system comprises Cas12b protein, specific sgRNA with the sequence shown as SEQ ID NO: 2 and a single-chain DNA reporter probe marked by fluorescence; when a CSBV target exists, the activated Cas12b protein trans-cleavage reporter probe generates a detectable fluorescence signal, so that rapid and high-sensitivity detection of the CSBV is realized. The invention further provides a detection kit suitable for the method. The detection kit comprises the primer pair, sgRNA and Cas12b protein. When the CSBV is detected based on the method and the kit, complex instruments are not needed, the operation is simple and convenient, the detection time is shorter than 1 hour, the sensitivity and the specificity are high, and the method and the kit are suitable for onsite rapid detection in a bee field.

The invention discloses a semiconductor resistivity nondestructive testing method and system based on modulation light reflection, relates to the technical field of semiconductor resistivity detection, and aims to accurately measure a modulation component delta R / R of light reflectivity through a light reflection modulation signal so as to calculate the semiconductor resistivity and further realize quantitative measurement of the semiconductor resistivity. The sensor has ultrahigh sensitivity, can detect tiny carrier concentration change, and is very sensitive to electrical properties of surface and near-surface areas; meanwhile, the spatial resolution is high, and the micron-scale light spot at the sample is suitable for micro-area measurement; a complete optical method does not cause any damage to the sample; various types of samples can be measured; the measuring speed is fast, and the system can be easily integrated in a production line for real-time monitoring.

This invention discloses an analytical method for detecting the content of 2,5-dihydroxybenzenesulfonic acid-1-hydroxypropyl ester in pharmaceutical creams using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS). The detection steps are as follows: A certain amount of pharmaceutical cream is transferred to a volumetric flask, a certain amount of methanol is added, and the mixture is heated to disperse it evenly. After cooling to room temperature, the mixture is brought to a final volume, shaken, and centrifuged. The supernatant is then used for detection, and the results are calculated using the external standard method. The detection ions used in this method are 247.1>189.2 and 247.1>139.9. This method is rapid, simple, accurate, reliable, and highly sensitive, and can be used to detect the content of 2,5-dihydroxybenzenesulfonic acid-1-hydroxypropyl ester in creams.

This invention discloses a search and rescue system and method for unmanned aerial vehicles (UAVs) equipped with odor sensors, relating to the field of gas detection technology. The system includes the following steps: installing and fixing the UAV-equipped odor sensor search and rescue device in a mountainous search and rescue area; establishing a power connection; setting the antenna orientation and air inlet layout; creating an installation list based on terrain undulations and wind direction distribution; and generating a UAV operating time reference. This invention achieves stable airflow and humidity balance through highly sensitive odor collection and stable flow and humidity control, ensuring continuous and accurate odor signal detection. Simultaneously, by combining the dynamic correlation between odor concentration and spatial location, the ground terminal generates trajectory adjustment commands to control the UAV to correct its flight attitude and sampling path in real time, forming a continuous odor tracking mechanism. This enables rapid target location and efficient search and rescue in complex mountainous environments.

This invention discloses a real-time data anomaly monitoring and repair method based on machine learning, comprising the following steps: S1, constructing data fragments and generating a consistent constraint set; S2, performing self-consistent representation construction; S3, performing anomaly detection and generating anomaly label set; S4, performing masking processing to generate masked data fragments, inputting them into an improved UniTS model, introducing a structural perturbation suppression mechanism, and generating a repair candidate set; S5, performing structural consistency evaluation and screening target repair data that meets the minimum structural perturbation condition; S6, performing data repair and generating repaired data fragments; S7, feeding back the updated consistent constraint set to the improved UniTS model. This invention achieves highly sensitive real-time identification of anomalous data items, effectively reducing the anomaly false judgment rate and repair distortion rate, and improving the data security, stability, continuity, and reliability in high-concurrency data stream environments.

This invention relates to the field of magnetic field detection technology and discloses a magnetic field detection method using a CrTe / ZnTe semi-metallic heterojunction grating structure. The method involves establishing a CrTe / ZnTe semi-metallic heterojunction grating structure through material preparation processes and detector operating frequency bands. A magnetic field is then applied to the CrTe / ZnTe grating structure, establishing a theoretical model that correlates the magnetic field strength with the response spectrum characteristics. The designed structure is fabricated using an etching process, and the sample is then packaged and tested. The invention proposes designing a CrTe / ZnTe semi-metallic heterojunction grating structure and utilizing changes in the response spectrum of the CrTe / ZnTe grating structure to detect the magnitude of the external magnetic field. Even slight changes in the magnetic field can affect the magnetoresistance and other properties of the CrTe / ZnTe material, thereby further altering the resonant frequency of the response spectrum of the CrTe / ZnTe grating structure. This method effectively improves the sensitivity, resonant mechanical properties, and temperature stability of magnetic field detection, providing a novel solution for highly sensitive weak magnetic field detection.

This invention proposes a speech anti-spoofing detection method and apparatus based on enhanced temporal and channel modeling. The method includes: inputting a speech signal into a pre-trained self-supervised speech model to obtain the hidden state features of K intermediate and output layers in the self-supervised speech model; performing nested feature enhancement processing on the state features of each layer to obtain the sentence-level representation vector corresponding to that layer; inputting the sentence-level representation vectors of all layers, calculating the weights of each layer, and generating the final fused embedding vector; inputting the final fused embedding vector into a classifier to output the discrimination result of whether the corresponding speech is real speech or synthesized speech. In the temporal dimension, this invention effectively captures the long-range temporal dependencies of speech by utilizing a local window multi-head self-attention mechanism, and is highly sensitive to inter-frame inconsistencies in synthesized speech; in the channel dimension, through channel partitioning and gating mechanisms, it fully explores the discriminativeness of the feature channels of each layer of the SSL model, and can also improve the model's stability and sensitivity.

This invention relates to an IL5 / Bi2MoO6 / MXene photoactive composite material, a low-background photoelectrochemical aptamer sensor, its preparation method, and its applications. The sensor is constructed by sequentially modifying chitosan, glutaraldehyde, complementary DNA strands, and a gold nanoparticle-labeled aptamer. A fixed capture probe and APT-Au synergistically achieve dual signal quenching, constructing an extremely low detection baseline. When the target analyte MUC1 is present, its specific binding to the aptamer causes APT-Au to detach, dissolving the dual quenching and significantly restoring the photocurrent, thereby achieving quantitative detection. This invention solves the problems of low efficiency, heterojunction instability, and high baseline in single quenching modes of existing photoelectric materials, achieving highly sensitive, highly specific, rapid, and low-cost detection of the breast cancer biomarker MUC1 in human serum samples, providing a novel, industrially feasible technical solution for large-scale early screening of breast cancer.

This invention provides a method, system, and electronic device for protecting the operational mode of model inference, relating to the field of artificial intelligence security technology. The method includes: generating a public sample set based on the original model; randomly selecting sensitive samples and copying the query sample to be inferred multiple times, mixing them, and then randomly shuffling them; encrypting the mixed dataset using a homomorphic encryption algorithm and sending it to a remote system for encrypted inference; finally, decrypting and reconstructing the returned encrypted inference result locally, and using double verification to determine whether the operational mode of the remote system is complete. This invention, by generating highly sensitive samples and combining sample copying, random shuffling, and homomorphic encryption algorithms, achieves efficient and reliable detection of tampering with the operational mode of the remote model's inference process, while simultaneously ensuring data privacy.

A fully integrated micro search coil magnetometer (μSCM) with a low-noise instrumentation amplifier is provided for low-frequency magnetic field measurements in the range of 1Hz to 1kHz. A micro search coil formed by stacking planar spiral inductors and serially connecting them with an in-phase configuration is used for magnetic field measurements. The critical line width and inner diameter of a planar spiral inductor are theoretically calculated and experimentally verified, enabling the development of a unique CMOS layout. This layout incorporates the calculated critical inner diameter, significantly optimizing the area utilization on the CMOS die. Additionally, the CMOS die includes a polysilicon-layer inductor for on-chip calibration, a Hall effect sensor for DC magnetic field measurement, and a temperature sensor for enhanced functionality. The μSCM delivers a compact, energy-efficient, and highly sensitive solution for low-frequency magnetic field measurements, surpassing the performance of most commercially available Hall effect sensors.

This invention discloses the application of platelet-reactive protein-4 (THBS4) in the diagnosis and treatment of endometriosis. Through bioinformatics integration analysis, clinical sample validation, and in vitro and in vivo experiments, this invention confirms that THBS4 is significantly highly expressed in both ectopic lesions and peripheral blood of patients with endometriosis, and its expression level is positively correlated with disease severity. The area under the receiver operating characteristic (AUC) curve for THBS4 alone in diagnosing endometriosis is 0.930, significantly superior to cancer antigen 125 (CA125); the AUC for the combined diagnosis of THBS4 and CA125 reaches 0.968. Simultaneously, silencing the THBS4 gene effectively inhibits the proliferation, migration, and invasion of human endometrial stromal cells, and significantly reduces the volume and fibrosis area of ​​ectopic lesions in a mouse model of endometriosis. This invention provides a highly sensitive and specific new biomarker for the non-invasive or minimally invasive early diagnosis of endometriosis, and provides new targets and candidate drugs for non-hormone-dependent targeted therapy.

This invention discloses a highly sensitive detection method for carbendazim based on terahertz metamaterial resonance enhancement. The invention proposes a terahertz metamaterial sensor with a "+" shaped composite double-peak structure. Transmission spectra were acquired using terahertz spectroscopy, and the differences in terahertz spectra at different concentrations were investigated. Analysis revealed that the amplitude of the transmission peak in the terahertz transmission spectrum of this structure decreases with increasing carbendazim solution concentration. Data preprocessing was performed using multivariate scattering correction (MSC), feature extraction was conducted using the iterative information-preserving variable (IRIV) method, and a quantitative detection model for carbendazim terahertz transmission spectra was established based on LS-SVM. This provides a new approach for the quantitative detection of carbendazim. Experimental results show that the model has a high R-value. P The assay yielded a value of 0.9825, an RMSEP of 0.2001, and a detection limit (LOD) of 0.672 μg / ml. This invention provides a novel method for the rapid, non-destructive, and highly sensitive detection of carbendazim in food.

This invention discloses a high-performance tin-based perovskite photodetector, its fabrication method, and its application in covert optical information transmission. The photodetector sequentially comprises a conductive substrate, an electron transport layer, a tin-based perovskite layer, a hole transport layer, and a metal electrode layer. The tin-based perovskite layer is FASnI3, and 1,4-butanediamine hydroiodate is introduced as a molecular additive to suppress Sn. 2+ Oxidation and reduction of defect state density optimize film quality. Based on this, FASnI3 perovskite films with an inverse opal structure were successfully prepared. The slow photon effect significantly enhances perovskite light absorption and promotes carrier transport, and the "lotus leaf effect" introduced by the photonic crystal significantly improves the stability of Sn-based perovskites. The photodetector prepared by this invention achieves highly sensitive, self-powered near-infrared detection under zero bias conditions, exhibiting high responsivity, high detectivity, low dark current, and excellent stability. It can be used in optical communication, information transmission, sensing, and security optoelectronic systems.

The invention belongs to the technical field of advanced materials and glucose detection, and relates to a 2D-MOF and MXene composite material, a printing type enzyme-free glucose sensor as well as a preparation method and application of the printing type enzyme-free glucose sensor. The preparation method of the composite material comprises the following steps: carrying out amination modification treatment on the MXene material; the MXene material subjected to amination modification treatment, nickel salt, BPY, TCPP and PVP are added into a mixed solvent of ethyl alcohol and DMF to be evenly mixed, and then a solvothermal reaction is conducted. The composite material has high catalytic activity, excellent conductivity and good dispersity, can be firmly attached to a flexible substrate, is high in stability, and can realize rapid and repetitive detection of glucose. The material can be prepared into conductive ink with good rheological property, is suitable for a printing process, can be used for preparing a wearable enzyme-free glucose sensor with high sensitivity and interference resistance, and has large-scale low-cost production potential.

The application belongs to the technical field of biological detection, and particularly relates to a visual detection kit for specifically diagnosing Theileria annulata under constant temperature conditions; the kit comprises RPA reaction products, crRNA, Cas13a protein, RNase inhibitor, reporter molecule, T7 RNA polymerase, NTP and NTT, the RPA reaction products are obtained by amplification of a primer pair shown in SEQ ID NO. 4 and 5, and the sequence of the crRNA is shown in SEQ ID NO. 1; the method is that the RPA reaction products, the crRNA, the Cas13a protein, the RNase inhibitor, the reporter molecule, the T7 RNA polymerase, the NTP and the NTT are mixed and incubated at an isothermal temperature; the incubation product is transferred into a detection buffer, inserted into a test strip, and the result is observed after incubation. The detection method has high sensitivity and high specificity, and can be used for specific detection of Theileria annulata.