751 results about "Highly sensitive" patented technology

The invention relates to the technical field of power system monitoring and fault diagnosis, and particularly discloses a fault diagnosis system of an electrical variable measurement insulator detection device. The system comprises a synchronous acquisition module, a multi-physical field feature extraction module, a self-adaptive fault diagnosis engine and a feedback module. By synchronously acquiring voltage harmonic waves, leakage current and temperature data, extracting feature vectors of coupling electric-thermal influence and performing two-stage collaborative diagnosis by using a dynamic threshold value and a mahalanobis distance, high-sensitivity and self-adaptive accurate identification and early warning of an early latent fault of the insulator are realized. According to the system, by constructing a synchronous data acquisition module and a multi-physical field feature extraction module, two key influence factors, namely an operation voltage harmonic component and an environment temperature, are brought into a diagnosis system in a quantifiable feature form for the first time.

The invention discloses a static database self-adaptive fuzzification desensitization implementation method for highly sensitive data in the power industry. The method comprises the following steps: a data preprocessing module, a sensitivity analysis module, a fuzzification algorithm module, a self-adaptive mechanism module and an effect evaluation and verification module. Compared with the prior art, the method has the advantages that 1, self-adaptive dynamic adjustment is performed: a desensitization strategy is automatically optimized according to data characteristics and use scenes, and privacy protection indexes (such as k-anonymity values) are improved by 30%-50%; 2, multi-dimensional balance: the information loss rate is reduced by 10%-20%, the query accuracy is improved by 5%-10%, and safety and service requirements are considered; 3, system expansibility: a plug-in architecture supports dynamic loading of new algorithms and rules, and a configuration management module realizes strategy real-time adjustment; and 4, compliance guarantee: through a law and regulation rule base and a compliance check algorithm, the compliance is ensured to meet the standard.

The invention relates to the technical field of cosmetics, and discloses an anti-inflammatory soothing cosmetic composition which comprises the following components in percentage by weight: 0.5%-4% of a barrier repairing component, 0.5%-3% of an immunoregulation component, 0.3%-2.5% of a nerve soothing component, 0.3%-3% of an anti-inflammatory plant compound, 1%-10% of a moisturizing soothing enhancer and the balance of auxiliary materials. According to the components, through a five-dimensional synergistic mechanism of barrier repair, immune tolerance, nerve desensitization, anti-inflammatory itching relieving, moisturizing and stability maintaining, anti-inflammatory relieving and barrier repair are remarkably enhanced; ceramide, rare ginsenoside, phytosterol ester and free fatty acid are introduced to synergistically construct a bionic liposome which is closer to the composition of human cuticle lipid, so that the transdermal absorption and stability are enhanced, and the repair efficiency is remarkably improved; the dipotassium glycyrrhizinate, the tamarix chinensis flower extract, the purslane extract, the dinoflaxanthine and the baicalin cooperate to quickly resist inflammation and relieve itching and strengthen barrier repair, so that the composition is suitable for highly sensitive skin.

The invention discloses a digital cultural tourism management system based on multi-source data analysis, and the system comprises a multi-source heterogeneous data collection module which is used for collecting tourist behavior data, environment data, cultural resource data and third-party platform data in real time; the user demand intelligent analysis module is used for constructing a dynamic user portrait through the multi-modal interaction data and identifying dominant and implicit demands; the culture knowledge graph construction module is used for generating a reasonable multi-dimensional knowledge network based on culture resource attributes and historical data; the travel route dynamic generation module is used for generating a personalized touring route in combination with the user portrait, the real-time environment and the resource state; according to the digital cultural tourism management system based on multi-source data analysis disclosed by the invention, the utilization rate of cultural resources is greatly improved, and the decision response speed is increased; the cultural cognition depth of tourists is improved, and the residence time of the tourists is prolonged; the damage rate of high-sensitivity cultural relics is reduced; and the special group culture acquisition efficiency is improved in a breakthrough manner.

This invention is related to the field of biosensors. In particular, devices and methods are described which enable the quantification of biomolecules by virtue of particle speed driven by induced-charge electrophoresis. For example, a range of biomolecules can be simultaneously detected in a highly sensitive manner from a library (e.g., combination) of active particles having different shapes or fluorescent signatures. Such biomolecules are related to conditions including, but not limited to, cancer biomarkers, viral infection biomarkers, toxins, pesticides, and small molecule drugs.

To improve allele discrimination, the inventors adapted the system combining Cas13a detection sensitivity with allele-specific PCR amplification to propose CASPER (Cas13a Allele-Specific PCR Enzyme Recognition) as a new versatile, easy-to-implement, and highly sensitive method to detect low-frequency of sequence variant. CASPER enabled specific and sensitive detection of KRASG12D with low DNA input such as DNA extracted from patient's pancreatic ultrasound-guided fine-needle aspiration fluids. CASPER is easy to implement and a versatile reliable method virtually adaptable to any point mutation.

The invention provides a patient abnormal state identification monitoring early warning method and system, and relates to the technical field of patient state monitoring. The method comprises the following steps: acquiring a facial image sequence of a patient, tracking a texture direction and constructing a variation comparison sequence, sorting linkage differences to generate dynamic clues, extracting non-shielding track mark picture distribution, identifying life fluctuations in a segmented manner, generating a fluctuation list through cross pairing, establishing a mapping chain to mark centralized anomalies, and generating an early warning scheme. According to the method, the recognition precision of facial subtle changes is improved, region credibility is calibrated in combination with a shielding ratio, non-shielding continuous image regions are screened, a physiological signal change sequence is segmented through trend continuity and direction consistency, high-sensitivity fluctuation paragraphs are extracted, and the credible image regions and physiological data are subjected to cross pairing in time and space; and merging intersection signals, identifying continuous high-frequency abnormal segments, and constructing a multi-dimensional mapping chain, thereby realizing high-precision early warning and real-time monitoring of complex state changes.

The invention discloses a preparation method of a recyclable electric sensing coating for defect detection. The preparation method is characterized by comprising the following steps: S1, preparing an electroluminescent unit EL; s2, preparing field coupling microspheres FCM based on an electroluminescent unit EL; and S3, preparing the recoverable electric sensing coating based on the field coupling microsphere FCM. The method has the advantages that electric field sensing and an environmental protection concept are successfully combined, and a low-cost, high-sensitivity and lossless polymer interface defect detection method is created. By visualizing the distribution of the electric field around the defect, the REC not only solves the bottleneck of the traditional technology in the aspects of detection precision, economy and environmental protection (recycling), but also provides a new way for realizing the fine management and maintenance of the material state, and has far-reaching significance for promoting the stable operation in the fields of electrical equipment and electronic equipment.

The invention discloses a high-sensitivity oxygen-resistant anode MFC (Microbial Fuel Cell) sensor for biotoxicity detection and a preparation method thereof. The sensor comprises a positive electrode, a negative electrode, a positive electrode chamber, a negative electrode chamber, a diaphragm, a current loop and a data acquisition system, a double-layer biological membrane is formed on the positive electrode through aerobic domestication, the outer layer is an aerobic bacteria layer, and the inner layer is an anaerobic electroactive bacteria layer. The aerobic bacteria on the outer layer consume dissolved oxygen and provide a stable environment for the anaerobic bacteria on the inner layer, so that the oxygen resistance and the operation stability of the sensor are remarkably improved. When the toxicity of a to-be-detected sample is detected, the biotoxicity of the sample is represented through the electrogenesis inhibition rate of the sensor. Experiments show that the response sensitivity of the sensor to toxic substances (such as Cu (II)) is obviously higher than that of a traditional anaerobic MFC sensor, and the sensor shows excellent anti-interference capability under different dissolved oxygen conditions. The method is suitable for rapid detection of biotoxicity of water and environmental samples, and has the characteristics of high sensitivity, strong oxidation interference resistance and good stability.

The invention provides a sewage biological treatment model prediction control method. According to the method, firstly, parameters are sorted through global sensitivity analysis, and high-sensitivity parameters are emphatically processed; dynamically evaluating the local sensitivity of the parameters based on a multi-scale time window; the DDQN network based on sensitivity guidance is fused with data-driven learning and physical mechanism knowledge; and the model prediction control system realizes multi-objective optimization of stable and up-to-standard effluent quality and energy-saving operation of the system based on the calibrated ASM3 model. According to the method, a complete closed-loop system from sensitivity analysis to parameter correction to prediction control is constructed, and compared with a traditional off-line parameter correction and experience control method, the model prediction precision, the control response speed, the operation stability, the energy efficiency level and the like are remarkably improved; and a complete technical support is provided for intelligent operation of an activated sludge process.

The invention discloses a divalent nucleic acid aptamer fluorescence detection method based on an exonuclease I auxiliary target circulation strategy, and belongs to the field of analysis and detection.The divalent nucleic acid aptamer is a repetitive sequence composed of two monovalent nucleic acid aptamers and can form double-stranded DNA through base complementary pairing with complementary strand cDNA of the divalent nucleic acid aptamer, and the divalent nucleic acid aptamer can be used for fluorescence detection of the divalent nucleic acid aptamer. The method comprises the following steps: taking a bivalent nucleic acid aptamer as a template, forming a DNA silver nanocluster (DNA-AgNCs) under the action of silver nitrate and sodium borohydride, and when the target okadaic acid exists, combining the bivalent nucleic acid aptamer with the target, so that cDNA can be replaced from double-stranded DNA by the target. Exonuclease I is added to perform enzyme digestion on the cDNA and the divalent nucleic acid aptamer, so that the fluorescence intensity of the DNA-AgNCs can be rapidly reduced. The content of the okadaic acid can be judged by detecting the change of the fluorescence intensity. The invention provides a simple, convenient, rapid, high-sensitivity and high-specificity detection method for okadaic acid detection based on a divalent nucleic acid aptamer for the first time.

The invention discloses an alginate-based high-entropy metal oxide as well as a preparation method and application thereof, and belongs to the technical field of gas sensor materials. The preparation method comprises the following steps: mixing a high-entropy metal salt solution of at least five metal sources with an alginate solution to prepare alginate-based high-entropy metal aerogel, and then wrapping the alginate-based high-entropy metal aerogel with carbon cloth and rapidly heating the alginate-based high-entropy metal aerogel by using a Joule thermal method to obtain the nanosheet-shaped alginate-based high-entropy metal oxide. The material can be used for high-sensitivity detection of ethanol gas. The preparation method provided by the invention is green, simple and rapid, has a good sensing response effect in ethanol gas detection, and the sensing material has good stability, can be recycled for multiple times, and has a good application prospect.

The invention discloses a composition, a detection method and a kit for SpaceAS variable splicing space in-situ detection of nucleic acid. The composition comprises a pi-type probe, a U-type probe, an O-type probe, an S-type probe and a signal probe, and the O-type probe adopts an in-vitro cyclization strategy, so that the problem of low hybridization efficiency caused by dependence on enzymatic cyclization reaction in a traditional hybridization method is effectively solved; meanwhile, an efficient probe hybridization system, a high-sensitivity signal amplification mechanism and a multi-round iterative imaging process are combined, so that the detection sensitivity and specificity are remarkably improved. The detection method has the advantages of high efficiency, high sensitivity, high specificity, good repeatability, strong expandability and the like through the synergistic effect of five-step single-stranded DNA probe hybridization reaction and one-step DNA amplification reaction, and can be widely applied to space in-situ detection and analysis of variable splicing events.

The invention discloses a hemiplegia rehabilitation intelligent evaluation method based on dynamic neighborhood firework optimization. The method comprises the following steps: generating a standardized multi-source hemiplegia rehabilitation data set; constructing a to-be-self-supervised training rehabilitation heterogeneous graph based on the standardized multi-source hemiplegia rehabilitation data set; using an improved decoding enhanced mask self-supervised graph learning device to carry out mask coding processing on the rehabilitation heterogeneous graph to be self-supervised and trained and the mask configuration set to obtain a masked rehabilitation heterogeneous graph representation; obtaining an initial rehabilitation score mapping model; forming a multi-target fitness function; generating a candidate spark parameter set; outputting an optimal parameter configuration set; and performing scoring reasoning on the time sequence consistent potential representation set by using the optimized rehabilitation scoring model. According to the method, comprehensive characterization which is highly sensitive to medium and long-term rehabilitation trend change and insensitive to accidental abnormity can be output, and the stability is obviously improved under real clinical multi-modal data.

This invention belongs to the field of biodetection technology, specifically relating to a monoclonal antibody 3C7 for detecting BK virus VP1 protein and its applications. The amino acid sequences of the heavy chain variable regions CDR1, CDR2, and CDR3 of the monoclonal antibody are SEQ ID NO. 1–3, and the amino acid sequences of the light chain variable regions CDR1, CDR2, and CDR3 are SEQ ID NO. 4–6, respectively. This antibody exhibits high specificity and can recognize the BK virus VP1 protein. The test strip includes a backing plate, a sample pad, a colloidal gold pad, a nitrocellulose membrane, and absorbent paper. The detection method provided by this invention is simple to operate, highly sensitive, and requires no instruments, enabling rapid and visual detection of BK virus VP1 in urine or recombinant protein samples. It is suitable for on-site screening and point-of-care testing and can supplement existing detection methods such as nucleic acid testing.

The invention relates to the technical field of environment control, and discloses a time-sharing optimization control method for a cold storage central air conditioner based on a large model, which comprises the following steps of: firstly, acquiring a system cold demand quantity and an environment working condition predicted value at a future moment through a pre-trained deep learning model, and estimating a current cold storage charging and discharging state in combination with a thermal front degradation correction algorithm; then, a non-linear flow resistance model associated with the porosity and the cold storage state is established, and a hydraulic high-energy-consumption interval in which fluid resistance is highly sensitive to flow change is recognized; and on the basis, deducing the energy efficiency trend of the water chilling unit by utilizing the environment temperature to determine load distribution, actively inhibiting cold storage output in a high flow resistance interval through energy efficiency gating logic, and finally generating a time sequence control strategy containing flow and load instructions to realize optimal scheduling of system-level energy efficiency.

The invention discloses a multi-terminal traveling wave fault positioning method, system and device of a branch type power distribution network and a medium, and relates to the technical field of power system protection and control, and the method comprises the steps: constructing a static topological graph of a power grid, and constructing a reference node distance matrix; collecting traveling wave signals of the monitoring points; performing empirical mode decomposition on the acquired traveling wave signal to decompose the traveling wave signal into an intrinsic mode function component; screening all the intrinsic mode function components, selecting an optimal intrinsic mode function component, and executing Hilbert-Huang transform to lock the arrival time of the traveling wave head; calculating the distance difference between the fault point and each monitoring point, and constructing an average fault distance difference matrix; and constructing a fault branch judgment matrix based on the average fault distance difference matrix and the reference node distance matrix. According to the method, the problem that a traditional impedance method is highly sensitive to line parameters and transition resistance is solved, and the defect that a single-end traveling wave method is prone to generating positioning ambiguity in a branch network is overcome.

The invention discloses a privacy enhancement continuous learning method based on lexical-level differential privacy and memory shaping, which is characterized by comprising a lexical-level dynamic differential privacy module and a privacy guide memory shaping module, the lexical element level dynamic differential privacy module adaptively allocates privacy budget according to semantic sensitivity of different lexical elements in a text, and introduces Gaussian noise in an embedded layer; the privacy guide memory shaping module comprises memory regularization and privacy perception forgetting; the memory regularization is used for dynamically constraining model parameters; the privacy aware forgetting is used to selectively impair memory for highly sensitive information. Compared with the prior art, the method has the advantages that higher accuracy and lower forgetting rate are maintained while privacy is guaranteed, forgetting of sensitive information and long-term maintenance of general knowledge are realized, disastrous forgetting in continuous learning is effectively relieved, and the method is applicable to privacy sensitive application scenes such as recommendation systems, medical diagnosis and financial analysis.

This invention provides a CRISPR / Cas12a fluorescence detection method for FocTR4 based on magnetic bead-ssDNA-HRP probes, belonging to the field of nucleic acid detection technology. This invention involves isothermal amplification of the FocTR4 target sequence using RPA primers; then, a magnetic bead-ssDNA-HRP probe is prepared; and the Cas12a / crRNA complex, RPA amplification product, and probe are co-incubated. The activated Cas12a cleaves the probe, releasing HRP. After magnetic separation, a colorimetric solution containing OPD is added, and the presence and concentration of FocTR4 are determined by detecting the fluorescence signal. This invention combines the high efficiency of CRISPR / Cas12a cleavage with the high sensitivity and strong specificity of RPA technology, achieving highly sensitive and specific detection of FocTR4, with a linear detection range of 10. 2 ~10 7 With a detection limit as low as 70 CFU / mL, it can quickly and accurately detect Foc TR4 in soil samples, enabling rapid on-site detection and providing reliable technical support for the early diagnosis and control of banana wilt disease.

This invention discloses a method for detecting *Salmonella typhimurium* based on magnetic covalent organic frameworks and aptamer-modified gold nanoparticles, belonging to the field of food safety technology. The method uses a magnetic covalent organic framework as a capture probe to capture *Salmonella typhimurium* present in the detection system. After incubation, the magnetic covalent organic framework-*Salmonella typhimurium* complex is collected by magnetic separation. Then, aptamer-modified gold nanoparticles are added, and after incubation, the complex formed by the magnetic covalent organic framework-*Salmonella typhimurium* complex and the aptamer-modified gold nanoparticles is collected by magnetic separation. Finally, a TPE-4A / hydrogen peroxide substrate solution is added, and the absorbance and / or fluorescence intensity are measured. Color parameters are extracted using a smartphone. This method is simple to operate, highly sensitive, and highly specific. Three detection modes work together for self-testing, resulting in accurate and reliable results.

This invention belongs to the field of gas sensor technology and discloses a method for preparing europium ferrite nanosensors and their applications. This method utilizes electrospinning technology combined with a specific low-temperature confined-domain calcination process (550℃~700℃) to prepare EuFeO3 nanotubes with a hollow structure and rough surface by taking advantage of the thermal decomposition kinetics differences of poly(p-v) magnets (PVP). This process effectively suppresses excessive repair of lattice oxygen while forming a unique tubular morphology, effectively "freezing" oxygen vacancies (O2) accounting for up to 34.44% on the material surface in situ. v This invention addresses the shortcomings of traditional solid fiber materials by constructing a sensor using a grinding, dispersion, and coating process. Utilizing the dual reactive surfaces of hollow nanotubes and high-concentration oxygen vacancy active sites, it achieves highly sensitive and selective detection of low-concentration ethylene gas released during fruit ripening. This invention solves the problems of small specific surface area and insufficient active sites in traditional solid fiber materials.

This invention discloses a near-infrared long-afterglow nanocomposite, its preparation, and its application in uric acid detection, belonging to the technical field of testing or analyzing materials by measuring their chemical or physical properties. The nanocomposite comprises a near-infrared long-afterglow luminescent center and an outer layer of organosilicon-doped mesoporous silicon containing tetrasulfide bonds. Under near-infrared light excitation, this nanocomposite exhibits excellent afterglow luminescence enhancement response to hydrogen peroxide. Since the product of uric acid decomposition by uricase is hydrogen peroxide, this composite enables highly sensitive and rapid detection of uric acid in blood. The composite provided by this invention has uniform particle size and high stability, effectively eliminating autofluorescence interference from biological components and improving detection accuracy.

This invention discloses a method for fabricating a highly sensitive long-wavelength infrared superconducting single-photon detector. It fully utilizes and amplifies the acoustic mismatch effect between the superconducting thin film and the substrate to achieve phonon localization enhancement on the superconducting nanowires. The effect of this innovative design is to increase the energy utilization rate of long-wavelength infrared photons on the superconducting nanowires, thereby improving the response sensitivity of the superconducting nanowires to long-wavelength infrared photon signals. Compared to existing designs that reduce the cross-sectional size of the superconducting nanowires, create holes beneath the nanowires, or increase the buffer layer, this invention is easier to implement and has higher scalability.

This application discloses a Mycoplasma pneumoniae P1 protein-binding protein, its preparation method, and its uses. The Mycoplasma pneumoniae P1 protein-binding protein includes an immunoglobulin single variable domain, wherein the immunoglobulin single variable domain comprises CDR1, CDR2, and CDR3 contained in VHH as shown in any one of SEQ ID NO: 1-8. The bivalent or multivalent nanobodies prepared using this Mycoplasma pneumoniae P1 protein-binding protein can effectively enhance their binding strength to antigens, providing a superior raw material for developing highly sensitive detection and diagnostic applications.

This invention relates to the field of chemiluminescence immunoassay technology, specifically to a magnetic bead coating method and its application for improving the luminescence level of p-tau 217. This invention optimizes the process of coating p-tau 217 capture antibodies with carboxyl magnetic beads. By gradient adjustment of the input ratio of EDC and NHS, the optimal activator concentration is obtained to enhance the magnetic bead coupling activity, increase the antibody coupling efficiency, and thus improve the signal values ​​of antigen samples and clinical samples. This invention has broad application prospects for the development of highly sensitive detection of low-abundance proteins such as p-tau 217.

This invention relates to a body fluid-based detection device for chronic diseases, which belongs to the field of medical devices. The device includes a body fluid detection fluid channel, a detection probe, a data processing module, and a data display module. The detection probe is disposed within the body fluid detection fluid channel, and the detection probe is connected to the data processing module, which is connected to the data display module. The body fluid detection fluid channels include: a body fluid ion detection channel, a body fluid pH detection channel, a body fluid hydrodynamic characteristic detection channel, a channel for detecting the content of soluble gases such as oxygen, a channel for detecting the physical characteristics of white blood cells and tumor cells, a channel for detecting the electrical characteristics of white blood cells and tumor cells, a channel for detecting the physical characteristics of red blood cells, a channel for detecting the electrical characteristics of red blood cells, a channel for detecting the electrical characteristics of separated body fluids, and a channel for detecting anticoagulation and fibrinolysis functions. This invention enables highly sensitive, non-invasive testing, providing a powerful tool for the early diagnosis of chronic diseases.

This invention discloses a homogeneous microsphere based on singlet oxygen self-circulating aggregation-induced emission, its preparation, and a wash-free detection method, which improves the sensitivity and efficiency of wash-free luminescent oxygen channel immunoassay. This invention successfully prepared AIE receptor microspheres by embedding the photosensitizer AIER dye, ultra-bright AIEG dye, and singlet oxygen-responsive dye SO into carboxyl-modified polystyrene nanoparticles. Simultaneously, an antibody-1-labeled AIE receptor microsphere solution, an antigen-containing test solution, and biotinylated antibody-2 were added to a 96-well plate, followed by streptavidin-modified donor microspheres. After wash-free incubation, the fluorescence signal value was measured, and the concentration of antigen in the test solution was obtained according to a standard curve. This platform completes wash-free, highly sensitive detection within 5 minutes, innovatively combining the aggregation-induced emission characteristics of AIEgens and a singlet oxygen cycling strategy, providing an efficient and universal solution for wash-free diagnostics.

A LiGa-based 0.5 In 0.5 A self-driven X-ray detector based on Se2 crystal and its fabrication method. This detector employs an MSM device structure with c-plane LiGa... 0.5 In 0.5 Using Se2 wafers as semiconductor materials and metal materials as electrodes, self-driven X-ray detection can be achieved under zero bias voltage; the above-mentioned self-driven X-ray detector fabrication method includes the following steps: (1) fabricating LiGa 0.5 In 0.5 (1) Se2 crystal; (2) Directional processing of c-plane LiGa 0.5 In 0.5 Se2 wafer; (3) LiGa 0.5 In 0.5 The upper and lower surfaces of the Se2 wafer are polished and passivated; (4) in LiGa 0.5 In 0.5 Metal electrodes are deposited on the upper and lower surfaces of a Se2 wafer. This invention utilizes LiGa... 0.5 In 0.5 The bulk photovoltaic effect generated by the non-centrosymmetric structure of Se2 enabled the fabrication of a self-driven X-ray detector with a simple structure. Furthermore, due to the LiGa... 0.5 In 0.5 Se2 crystals have high resistivity, and the devices fabricated from them have low dark current, enabling highly sensitive X-ray detection.

Method for manufacturing a flexible strain sensor, comprising: Placing a flexible substrate on a movable stage in a working chamber, in which the movable stage, suitable for a desired movement according to a control signal, and a nozzle, suitable for spraying towards an upper surface of the movable stage, are installed, and with which a first pressure control unit, suitable for controlling an internal pressure of the working chamber, is combined; and preparing a printing material mixture, which comprises metal nanoparticles and carbon nanotubes (CNTs) in a powder form, which is to be introduced into a printing material tank, which is provided with an upper outlet that communicates with a nozzle via a first communication line, and a lower inlet, with which a second pressure control unit, suitable for controlling pressure, is combined. Control, by means of a control unit, a movement of the movable stage by providing a predetermined motion control signal to the movable stage in order to move the flexible substrate at a desired speed along a path which corresponds to a predetermined conductor pattern of a strain sensor, Forming a relatively low-pressure atmosphere within the working chamber by operating the first pressure control unit and simultaneously a relatively high-pressure atmosphere at a lower inlet of the pressure material tank by operating the second pressure control unit, In parallel with controlling the movement of the movable stage, the printing material mixture is forcibly transmitted in an aerosolized state through the first communication line from the printing material tank to be sprayed through the nozzle towards a surface of the flexible substrate by means of a compression wave, which is caused by a pressure difference between the low-pressure atmosphere and the high-pressure atmosphere. Direct printing of a predetermined conductor pattern of the strain sensor onto the flexible substrate by a process in which the printing material mixture, which is extruded through the nozzle, collides with the surface of the flexible substrate to create cracks on the surface, and the CNTs penetrate into the cracks and are mechanically anchored to the flexible substrate, and then the following metal nanoparticles and CNTs of the printing material mixture are deposited in a predetermined width and height on the surface of the flexible substrate. Connecting a first and a second lead wire, which extend electrically to protrude from the flexible substrate, to both ends of the predetermined conductor pattern of the strain sensor and Binding a flexible cover, which has the same size as the flexible substrate, to the surface of the flexible substrate on which the predetermined conductor pattern is printed, so that the predetermined conductor pattern is inserted between the flexible substrate and the flexible cover.

The invention relates to the technical field of virus detection, and discloses a method and a kit for detecting Chinese sacbrood virus. The method comprises: firstly, carrying out isothermal amplification on a CSBV specific target sequence in a sample nucleic acid by using an RPA technology, the RPA technology adopting a specific primer pair, the sequences of which are SEQ ID NO: 4 and SEQ ID NO: 5; then, an amplification product is mixed with a CRISPR / Cas12b detection system, and the system comprises Cas12b protein, specific sgRNA with the sequence shown as SEQ ID NO: 2 and a single-chain DNA reporter probe marked by fluorescence; when a CSBV target exists, the activated Cas12b protein trans-cleavage reporter probe generates a detectable fluorescence signal, so that rapid and high-sensitivity detection of the CSBV is realized. The invention further provides a detection kit suitable for the method. The detection kit comprises the primer pair, sgRNA and Cas12b protein. When the CSBV is detected based on the method and the kit, complex instruments are not needed, the operation is simple and convenient, the detection time is shorter than 1 hour, the sensitivity and the specificity are high, and the method and the kit are suitable for onsite rapid detection in a bee field.