443 results about "Molecular imprinting" patented technology

The invention discloses a method for constructing a dual-mode sensor through smartphone-assisted surface molecular imprinting for detecting diphenyl phosphate and application of the dual-mode sensor, and belongs to the technical fields of biochemical analysis, functional nano materials and life science. In synthesis, solubility and selectivity of a target object and photo-initiation efficiency in a polymerization process are optimized, and diphenyl phosphate molecularly imprinted nano-enzyme MIPs is rapidly prepared through a molecular imprinting technology; in the detection, H2O2 and 3, 3, 5, 5-tetramethyl benzidine TMB are catalyzed by peroxidase-like activity of Fe and N-CDs to generate a chromogenic reaction and an inner filter effect of oxTMB is used for inhibiting a fluorescence signal, dual-signal cross validation is realized in cooperation with a smart phone, and the colorimetric-fluorescence sensor for detecting diphenyl phosphate is constructed. The sensor has specific recognition selectivity and binding affinity of diphenyl phosphate, is simple to operate and low in cost, can realize sensitive detection of diphenyl phosphate in a wide linear range, has the detection limit as low as 16 pg / mL, and is suitable for field analysis of trace DPhP in a complex water environment.

The invention constructs a universal visual virus molecularly imprinted sensor modified with an anti-protein coating, the sensor uses magnetic Fe3O4 as an imprinting carrier, HBV as a template and dopamine as a functional monomer for imprinting to obtain a molecularly imprinted polymer with a polydopamine coating (PDA), and before the template is removed, the molecular imprinting polymer with the polydopamine coating (PDA) is separated from the template, so that the molecular imprinting polymer with the polydopamine coating (PDA) is obtained. The surface of the PDA layer is covered with a slightly cross-linked PMEO2MA layer through a water precipitation polymerization method, and the polymer can effectively reduce non-specific protein adsorption; and finally, exposing an imprinting cavity of the PDA layer by eluting the template molecules. On the other hand, potassium permanganate (KMnO4) is used as a signal output molecule, and when no target object exists, the strong reducibility of PDA can enable KMnO4 entering the imprinting cavity to be subjected to a color fading reaction. Based on the principle, high-specificity colorimetric visual detection on the HBV is realized. The sensor can be combined with a smart phone through naked eyes to carry out visual semi-quantitative analysis and carry out quantitative detection through ultraviolet rays, and the universality of the sensor can be confirmed through a template replacement experiment. And meanwhile, the sensor shows an excellent cost advantage. The diagnosis which is high in sensitivity, low in cost and capable of achieving rapid visualization shows huge potential in practical application, and particularly has important application value in infectious disease screening and monitoring work in areas with limited resources.

The invention belongs to the technical field of analytical chemistry detection, and particularly relates to a construction method and application of a tobacco mosaic virus molecular imprinting sensor based on MIL-101 (Fe). The construction method comprises the following steps: by taking a tobacco mosaic virus TMV as a template virus, preparing a molecularly imprinted polymer MIPs with a TMV specific recognition cavity on the surface of MIL-101 (Fe) through a free radical polymerization reaction; the template virus is removed in the preparation process of the molecularly imprinted polymer MIPs, and a TMV specific recognition cavity of the molecularly imprinted polymer MIPs contains MIL-101 (Fe); the molecularly imprinted polymer MIPs is the tobacco mosaic virus molecularly imprinted sensor based on MIL-101 (Fe). Hydrochloric acid is introduced, so that the TMV specific recognition cavity structure of the molecularly imprinted polymer MIPs is cracked to release Fe < 3 + >, then potassium thiocyanate is added, and the Fe < 3 + > and KSCN are subjected to a complexation reaction to generate a red complex. And the complexation reaction of Fe < 3 + > and KSCN is used as a signal probe, the rapid semi-quantitative visual detection of the target virus TMV by naked eyes is realized, and quantitative detection and analysis are carried out through an ultraviolet spectrophotometer.

The invention discloses a dual-emission molecular imprinting ratiometric fluorescence sensor, a preparation method thereof and application of the dual-emission molecular imprinting ratiometric fluorescence sensor in aureomycin detection, and belongs to the technical field of chemical sensors. The preparation method of the sensor comprises the following steps: preparing silicon dioxide-quantum dot composite nanoparticles, and preparing a molecularly imprinted polymer by taking salicylamide as a template molecule; and mixing the molecularly imprinted polymer and the silicon dioxide-quantum dot composite nanoparticles in an HEPES buffer solution by adopting a post-mixing strategy to construct the dual-emission molecularly imprinted ratiometric fluorescence sensor. The double-emission molecular imprinting ratiometric fluorescence sensor has the beneficial effects that the double-emission molecular imprinting ratiometric fluorescence sensor provided by the invention is rich in fluorescence color change, rapid, high-sensitivity and visual detection of aureomycin can be realized, the whole detection process is simple, convenient and rapid, and the double-emission molecular imprinting ratiometric fluorescence sensor is suitable for being widely popularized in practical application.

The invention belongs to the technical field of organic matter extraction, and particularly relates to a green directional extraction and purification method for haematococcus astaxanthin, which comprises the following steps: (1) mixing dried haematococcus powder and a composite pretreatment agent according to a mass ratio of 1: (8-15), and stirring to obtain a pretreated algae solution; (2) treating the pretreated algae liquid through ultrahigh-pressure microjet equipment to obtain wall-broken slurry; (3) mixing the wall-broken slurry and a composite extraction agent according to a volume ratio of 1: 3-1: 5, and performing extraction separation to obtain an astaxanthin extraction phase; (4) carrying out selective adsorption on the astaxanthin extraction phase through an adsorption column filled with a molecular imprinting adsorbent; (5) collecting eluent by adopting a gradient elution method; and (6) performing vacuum concentration on the eluent, and performing spray drying to obtain an astaxanthin product. According to the method, the astaxanthin extraction efficiency and the product purity are remarkably improved, meanwhile, the energy consumption is reduced to the maximum extent, toxic solvent residues are avoided, and the stability of the astaxanthin is effectively protected.

The invention discloses a cyclic regeneration treatment method of stainless steel pickling waste liquid, which realizes full-flow recycling of the pickling waste liquid by constructing a closed-loop system of pretreatment, adsorption, desorption, recovery, regeneration and circulation. A molecularly imprinted polymer adsorption technology is adopted, targeted capture of Cr < 3 + >, Ni < 2 + > and Fe < 2 + > / 3 < + > is achieved through specific recognition sites, and the bottleneck that a traditional adsorbent is poor in selectivity is broken through; the supercritical CO fluid extraction technology utilizes the characteristics of high diffusivity and low surface tension, efficient desorption of metal ions is achieved under mild conditions, and secondary pollution caused by a traditional organic solvent is avoided. A technical closed loop is formed among the steps through parameter synergy and substance circulation, and finally the dual purposes of full-amount regeneration of the pickling waste liquid and efficient recovery of metal resources are achieved.

The invention discloses a sialic acid molecular imprinting extraction method, and belongs to the technical field of separation and purification. The invention discloses a sialic acid molecular imprinting extraction method which comprises the following steps: dissolving a sialic acid standard substance and a functional monomer in a binary pore-foaming agent-ionic liquid, adding a cross-linking agent and an initiator, polymerizing under ultraviolet light to prepare a high-molecular polymer, and washing off the sialic acid standard substance to prepare a molecular imprinting polymer; specifically adsorbing sialic acid in the fermentation liquor by using the molecularly imprinted polymer; performing graded elution on sialic acid by using a methanol-water mixed solution and a methanol-acetic acid mixed solution; and performing rotary evaporation concentration, anti-solvent crystallization, separation and drying on the bird's nest acid eluent. Tests show that the sialic acid purity is greater than or equal to 99%, and can meet the requirements of the sialic acid in the fields of food, health care, medicine, cosmetics and the like. According to the method, sialic acid can be efficiently separated and extracted from sialic acid fermentation liquor, the product yield is increased, the extraction procedures are reduced, and the production cost and the discharge amount of three wastes are reduced.

The invention discloses a preparation method and application of nano-enzyme molecularly imprinted detection particles, a molecularly imprinted material is developed on the basis of a FeN3P nano-enzyme material, the molecularly imprinted material is fixed on filter paper to realize high-sensitivity and high-selectivity detection of 25-(OH) D3, real-time field detection of the molecularly imprinted material is realized by matching with a self-researched mobile phone APP: Visual Method, and the nano-enzyme molecularly imprinted detection particles have very high practical application value. Accurate data support is provided for early warning and personalized intervention of osteoporosis, community screening and long-term health management lack of 25 (OH) D3 can be promoted, and the method has important scientific value and social significance in reduction of the incidence rate of osteoporosis and optimization of medical resource allocation.

The invention relates to the technical field of quality detection, and provides a method for detecting the quality of an aminobutyric acid extract in peanut sprouts, which comprises the following steps: performing specific recognition on aminobutyric acid by a molecular imprinting sensor, and calculating the impurity contribution by combining a preset peanut sprout interference database and partial least square regression; the interference of complex matrix impurities in the peanut bud extract can be specifically eliminated, and the problem that the specificity is insufficient when a traditional method is used for detecting a complex sample is solved; the molecularly imprinted sensor and the quartz crystal microbalance are connected in series through the microfluidic channel, and resonance frequency detection is triggered by using capacitance variation, so that a coherent process is formed from identification to quality analysis of sample detection, and the detection efficiency is improved; the corrected frequency offset, the phase angle and the temperature are input into the recurrent neural network, accurate output of the concentration of the aminobutyric acid is achieved by integrating multi-dimensional detection information, compared with a single parameter detection method, the real content of the aminobutyric acid in an extract can be reflected more comprehensively, and a reliable basis is provided for quality judgment.

The invention discloses a molecularly imprinted adsorption material taking a metal organic framework as a carrier skeleton as well as a preparation method and application of the molecularly imprinted adsorption material, and belongs to the technical field of adsorption materials. The preparation method comprises the following steps: carrying out hydrothermal reaction on transition metal salt and a phthalic acid ligand to obtain metal organic framework nanoparticles; the preparation method comprises the following steps: adsorbing flavonoid template molecules by metal organic framework nanoparticles, mixing with lysozyme and a disulfide bond reducing agent, performing phase change polymerization, and eluting to remove the flavonoid template molecules after the phase change polymerization is completed, thereby obtaining the molecularly imprinted adsorbing material with abundant imprinting sites and good stability. The adsorbent is good in flavonoid compound adsorption selectivity, high in rate, large in capacity, good in chemical stability, resistant to acid washing, capable of being recycled for multiple times and capable of being widely applied to extraction and separation of flavonoid compounds.

The invention provides a method for detecting chloramphenicol residues in animal food by adopting a molecular imprinting sensor, which comprises the following steps: preparing the molecular imprinting sensor with specific recognition on CAP, and providing an electrochemical detection system of the molecular imprinting sensor for detecting the chloramphenicol residues in the animal food, fitting to obtain a calculation formula of chloramphenicol concentration detected by the molecularly imprinted sensor; treating an animal food sample, and mixing the animal food sample with a standard concentration CAP solution according to a volume ratio of 1: 9 to obtain a to-be-detected sample solution; scanning the to-be-detected sample liquid through the molecular imprinting sensor by adopting a square wave voltammetry method to obtain a peak current value; and obtaining the concentration of chloramphenicol in the sample solution to be detected according to the peak current value and a calculation formula of chloramphenicol concentration detected by the molecular imprinting sensor. The method is sensitive, accurate and practical.

The invention discloses a rhizoma polygonati polysaccharide rapid detection electrochemical sensor as well as a preparation method and application thereof, and relates to the field of electrochemical sensors. The method comprises the following steps: (1) preparing an AC-COOH-PANI composite material; (2) preparing an AC-COOH-PANI (at) GCE modified electrode; and (3) preparing the MIP-AC-COOH-PANI (at) GCE. According to the present invention, Glu is adopted as a template molecule, MAA is adopted as a functional monomer, a molecularly imprinted polymer film is constructed on the surface of an AC-COOH-PANI composite material modified GCE electrode through a self-assembly polymerization technology, and the molecularly imprinted electrochemical sensor MIP-AC-COOH-PANI (at) GCE for rapid detection of polygonatum sibiricum polysaccharide is developed, the sensor shows relatively low detection limit, relatively wide linear range, relatively high sensitivity and good selectivity and stability, and has a good application prospect.

The invention relates to the field of biological product preparation, in particular to a preparation method of high-activity oyster-derived small molecule peptide. The method comprises the following steps: selecting fresh meat of pacific oysters or ostrea rivularis as a raw material, degreasing, adding water, crushing, filtering, sequentially carrying out compound protease and neutral protease hydrolysis, implanting a sensor and a probe in hydrolysis, monitoring in real time, and dynamically adjusting reaction conditions; adding a targeting microcapsule wrapping an ascorbic acid glutathione composite protective agent, and releasing the protective agent when a target peptide signal is detected; separating and concentrating through a gradient membrane, and purifying the target peptide by using a molecular imprinting chromatographic column; carrying out alkaline hydrolysis on enzymatic hydrolysis residues to prepare chitosan for cyclic utilization, carrying out enzymatic hydrolysis on residual residues to generate collagen peptide, treating and recycling wastewater, and recovering protease. According to the method, the technical effects that the high-activity and high-purity target peptide is prepared, the resource utilization rate is increased, the production cost is reduced, the target peptide has good oxidation resistance and biological activity, the intestinal enzyme degradation rate is reduced after glycosylation modification, and the blood half-life period is prolonged are achieved.

The invention designs a preparation method of a magnetic molecular imprinting-aptamer sandwich fluorescence sensor for detecting kanamycin. And the magnetic molecular imprinting composite material Fe3O4 (at) UiO-66 (at) MIP with a specific adsorption target object KANA is prepared. When a detection target object KANA exists, the Fe3O4-coated UiO-66-coated MIP can rapidly and specifically capture the KANA in a short time. Then, a DNA single-stranded aptamer marked with FAM is added, after the aptamer and KANA captured on the surface of the Fe3O4 at-UiO-66 at-MIP are subjected to specific recognition and magnetic separation again, an aptamer probe can enter sediment along with the magnetic composite material Fe3O4 at-UiO-66 at-MIP, the fluorescence intensity of supernate is reduced, and quantitative analysis is conducted according to the fluorescence difference value delta F. When the KANA does not exist, the aptamer cannot enter the precipitate, so that the fluorescence signal intensity is not changed. Double recognition is formed through specific binding of an MIP imprinting cavity and the aptamer, matrix interference is reduced by means of fluorescence characteristics and magnetic separation capacity, the method has the advantages of being high in adsorption capacity, specific in recognition, high in sensitivity, wide in detection range, good in selectivity and the like, and rapid and accurate detection of KANA in a complex sample can be achieved.

The invention relates to a calcium-based desulfurizer and a preparation method and application thereof, and relates to the technical field of desulfurizers, the calcium-based desulfurizer comprises a calcium-based carrier with a micropore-mesopore-macropore three-level pore structure, and a gamma-aminopropyltriethoxysilane modifier dispersed in pore channels, the molecular imprinting layer is modified by an inactive region on the inner wall of the mesoporous; the preparation method comprises the steps of calcium-based carrier pretreatment, composite pore forming, pre-roasting, hydroxyl strengthening and the like. The efficient mass transfer system is constructed through a three-stage pore structure, the modifier and the molecular imprinting layer synergistically improve the structural stability and the sulfur dioxide adsorption selectivity, the calcium base utilization rate and the desulfurization efficiency are improved, and the industrial flue gas desulfurization requirement is met.

The invention belongs to the field of electroanalytical chemistry / electrochemical sensors, and discloses preparation and application of a molecularly imprinted sensitive membrane modified electrode for perfluorooctane sulfonic acid detection. According to the method, aniline and o-fluoroaniline are selected as bifunctional monomers, PFOS is taken as a template molecule, in-situ electropolymerization is carried out through cyclic voltammetry, and the molecularly imprinted sensitive membrane capable of specifically recognizing PFOS is obtained. According to the invention, o-fluoroaniline is introduced and is combined with a fluorocarbon chain of PFOS through F-F interaction, and due to the existence of fluorine atoms, non-specific combination can be reduced, so that the anti-interference capability of the molecularly imprinted sensitive membrane is stronger, and the selective limitation on a perfluorinated compound composed of a sulfonic acid group and the fluorocarbon chain is reduced; according to the invention, aniline is used as another functional monomer, so that the conductivity can be improved, and the aniline and o-fluoroaniline can be doped and electrically polymerized to form fluorinated polyaniline. And the chemical property of the fluorinated polyaniline is relatively stable, so that the stability of the eluted molecularly imprinted cavity is relatively high.

The invention provides a method for diagnosing early lung cancer based on a terahertz sensor array and an artificial intelligence technology. 16 terahertz sensors with the same structure and high-quality factors (Q value) are adopted to form a 4 * 4 array, and the 16 lung cancer markers are specifically detected through modification by a molecular imprinting technology. During detection, the modified terahertz sensor array is placed in a closed gas chamber made of polytetrafluoroethylene, nitrogen is firstly introduced to obtain a reference spectrum, then gas exhaled by a subject is introduced to obtain a test spectrum, and the concentration of the marker is reflected through the harmonic peak frequency shift amount. An initial diagnosis model is established in combination with individual information of a subject, and the model is trained and optimized by using an artificial intelligence technology and a large sample. According to the method, the problems of insufficient specificity, low sensitivity, high misdiagnosis rate of a single marker and the like in a traditional method are solved, and non-invasive, rapid and accurate diagnosis of the early lung cancer is realized.

The invention relates to the technical field of health wine preparation, and discloses a multi-effect synergistic enhanced bear gall body-building wine compound preparation process which comprises the following steps: taking sorghum, corn and fructus lycii as fermentation base materials, and carrying out double-strain synergistic fermentation by adopting saccharomyces cerevisiae and lactobacillus plantarum; adding fresh ursodesoxycholic acid during fermentation, carrying out in-situ enzymatic debinding conversion by using a compound enzyme system generated by a fermentation system, and converting the bound bile acid into free ursodesoxycholic acid; after fermentation, distilling to obtain raw wine; a molecularly imprinted polymer prepared by taking a wolfberry polyphenol key monomer as a template molecule is adopted to carry out targeted anchoring refining on raw wine, selective capture and enrichment of wolfberry polyphenol are carried out, and finally aging is carried out to obtain a finished product. According to the method disclosed by the invention, upstream double-strain fermentation in-situ enzymatic conversion and downstream molecular imprinting targeted refining are systematically integrated, so that the technical contradictions that the conversion rate of combined bile acid is low and a large amount of polyphenol functional components are synchronously lost during purification and impurity removal in a traditional process are solved.

The application discloses a kind of molecular imprinting cerium oxide / biochar composite material and its preparation method and application, comprising: cerium oxide / biochar composite material and solvent are mixed, ultrasonic, obtain first solution;At room temperature, first solution and imprinting polymer are mixed, stirring, obtain first mixture, imprinting polymer is the mixture of template molecule and functional monomer, template molecule is p-chlorophenol, and functional monomer is methyl methacrylic acid;First mixture is purged with N2, under sealed, ultraviolet light radiation polymerization 12-24h, obtain second mixture, second mixture is centrifuged, washes to detect no template molecule, clean, dry, obtain molecular imprinting cerium oxide / biochar composite material.Molecular imprinting cerium oxide / biochar composite material as catalyst in the application of photocatalytic removal of p-chlorophenol in water body shows higher specific selectivity and catalytic activity, and the removal efficiency of p-chlorophenol reaches 90%, with good anti-interference in complex water environment.

The application provides a composite imprinting polymer and a preparation method and application thereof, and belongs to the field of compound extraction.The composite imprinting polymer comprises a core, a shell and a shell;the core comprises Fe3O4 and SiO2 coated on the Fe3O4;the shell comprises MOF material and imprinting polymer coated on the MOF;raw materials for preparing the imprinting polymer comprise a template molecule, a functional monomer, a crosslinking agent and an initiator;the template molecule is quercetin;the functional monomer comprises two of methyl methacrylate, styrene, 4-vinylpyridine and acrylamide; and the crosslinking agent comprises ethylene glycol dimethacrylate.The material has the triple advantages of magnetic rapid separation, high specific surface area of MOF and specific recognition of molecular imprinting, and exhibits high adsorption capacity and excellent selectivity for quercetin.

The invention relates to the technical field of detection and identification of fentanyl substances, and discloses a selective quantitative detection method of fentanyl. According to the method, a differential pulse voltammetry (DPV) is used for making a linear relation diagram of a potassium ferricyanide peak current and fentanyl solutions with different concentrations to obtain a standard working curve, a linear equation is obtained through the standard curve, and the detection limit is 1.0 * 10 <-12 > mol.L <-1 >; the molecularly imprinted membrane modified electrode prepared by the invention is used for measuring the peak current of a potassium ferricyanide solution, so that fentanyl molecules can be indirectly and quantitatively analyzed and measured. The method is characterized in that a fentanyl molecularly imprinted membrane is electrically polymerized on the surface of a glassy carbon electrode, m-phenylenediamine is used as a functional monomer, and the selectivity and sensitivity of an electrochemical method for detecting fentanyl are improved. Meanwhile, by taking an adsorption pulse stripping voltammetry (DPASV) as a detection technology, a method capable of rapidly detecting fentanyl molecules in the presence of various drugs is established. The molecularly imprinted membrane electrode disclosed by the invention is simple to prepare, quick in response and good in selectivity and reproducibility, and can be repeatedly used for more than five times.

The invention discloses a Hedyotis diffusa extract, comprising a hedyotis diffusa glycoside content of 2500-4000 ppm, deacetylated hedyotis diffusa methyl ester, scutellariae glycoside methyl ester, and hedyotis diffusa glycoside content of 4500-6500 ppm, a total flavonoid content of less than 100 ppm, no characteristic color reaction for anthraquinone identification, hedyotis diffusa acid content of less than 100 ppm, deacetylated hedyotis diffusa acid content of less than 100 ppm, and geniposide acid content of less than 100 ppm, and a pH value of 6.0-7.0. The extract is obtained by vacuum ultrasound, centrifugation, and vacuum concentration, followed by acid adjustment to a pH of 2.5-3.0, followed by aging, membrane filtration, and purification by ion exchange column chromatography to obtain a permeate A and a Hedyotis diffusa alkaloid eluate J1. The permeate A is filtered through a membrane, separated by molecular imprinting polymerization adsorption, vacuum concentrated, and ultrafiltered. Finally, the pH is adjusted to 6-7 with J1, and the extract is sterilized by heat preservation.

The invention relates to a preparation method of a molecularly imprinted composite filler, and relates to the technical field of analytical chemistry and environmental monitoring technologies, the preparation method of the molecularly imprinted composite filler comprises the following steps: firstly dissolving template molecules and a first monomer, then adding a cross-linking agent and an initiator, carrying out deoxidation and water bath, and then centrifuging to obtain a template molecule solution; and eluting the precipitate with a flushing fluid, and drying to obtain the microspheres. And dissolving the ionic liquid, uniformly stirring with the obtained microspheres, and carrying out centrifugal separation, washing and vacuum drying to finally prepare the molecularly imprinted composite filler. Finally, the hydrophobic property of the molecularly imprinted composite filler is improved through modification of the ionic liquid and the second monomer. The method provided by the invention has excellent hydrophobic effect and enrichment capability on a target object.

A trimodal detection method for ethyl carbamate based on molecular imprinting regulation of Co@MOF-MIP enzyme activity switch, comprising the following steps: Step 1: Determine the absorbance value A of the mixed reaction solution of ethyl carbamate at 650nm n Step 2: Under the excitation wavelength of 365nm, the fluorescence intensity value F of the mixed reaction solution of ethyl carbamate at 440nm is measured. n Step 3: Determine the temperature T of the mixed reaction solution of ethyl carbamate under 660nm laser irradiation n :Step 4: Construct a linear regression equation for the concentration of ethyl carbamate; Step 5: Take the sample to be tested and repeat steps 1, 2 and 3 to obtain the absorbance signal of the colorimetric mode, the fluorescence intensity signal of the fluorescence mode and the temperature signal T of the photothermal mode n The synthesis process of Co@MOF‑MIP nanozyme is relatively simple, the raw materials are readily available and low-cost, and it does not require complex processes and expensive equipment. It has high stability and high peroxidase activity and fluorescence properties, which meet the needs of subsequent multimodal detection of EC.

The application discloses a temperature-sensitive molecular imprinting sensitive film, a preparation method thereof and application thereof in a glyphosate detection sensor, and belongs to the field of optical sensing technology and environmental monitoring. The application forms an imprinting cavity which is highly complementary to a template molecule in size and spatial configuration in a polymer network, realizes specific identification of a target molecule, and solves the problems of complex sample pretreatment and long detection period of the existing glyphosate detection method. The application introduces a temperature-sensitive material PNIPAM, realizes in-situ, non-destructive and reversible release of the template molecule by temperature control, discards the traditional destructive elution mode, avoids causing serious secondary environmental pollution, avoids the problems of swelling and corrosion of the imprinting cavity caused by strong acid or organic solvent, and causes irreversible damage to the structure of the imprinting cavity, which seriously affects the reuse frequency of the sensor and the detection stability.

The present application belongs to the technical field of adsorbing materials, and particularly relates to a titanium dioxide molecular imprinting polymer as well as a preparation method and application thereof. The present application uses Sudan red as a template molecule, acrylamide as a functional monomer, ethylene glycol dimethacrylate as a crosslinking agent, and azobisisobutyronitrile as an initiator to construct a cavity structure matching the Sudan red molecule on the surface of the amino-modified titanium dioxide ultrafine particles, and successfully prepares the titanium dioxide molecular imprinting polymer. The material combines the photocatalytic characteristics of the titanium dioxide and the selective recognition ability of the molecular imprinting, can degrade the Sudan red under light, and has a higher photocatalytic efficiency than that of the pure titanium dioxide. The material synthesized through the surface imprinting technology has a three-dimensional cavity complementary to the template molecule, and has a larger adsorption capacity than that of the non-imprinted material. The material realizes the integration of the selective recognition, adsorption and degradation of the Sudan red, and is suitable for complex industrial environments.

The invention discloses an interface molecularly imprinted polymer microsphere as well as a preparation method and application thereof, and relates to the technical field of functional polymer materials. The preparation method comprises the following steps: firstly, enabling template molecules to react with a silane coupling agent to form a template molecule compound; and then anchoring the template molecule compound on the surface of SiO2 particles, so that the template molecule compound simultaneously plays the dual roles of a stabilizer and an imprinting template in Pickering emulsion polymerization. And after the polymerization is completed, removing the template molecule modified SiO2 particles to obtain the polymer microspheres of which the imprinting sites are completely exposed on the surfaces of the microspheres. Molecular imprinting sites of the microspheres are uniformly distributed on the surface and are in an open and highly accessible state, so that the recognition rate and the binding capacity of target molecules are remarkably improved, and the microspheres have excellent selective enrichment and separation performance on target analytes; a reliable technical path is provided for practical application of the molecularly imprinted material in the fields of solid-phase extraction, sensing detection, medicine purification and the like.

The invention discloses a preparation method of a molecularly imprinted membrane assembly material, the molecularly imprinted membrane assembly material and a nanowire / nanosheet heterojunction, and belongs to the technical field of water pollution control. According to the preparation method, an Eu / CuO / Bi2O2Se nanowire / nanosheet heterojunction is constructed by taking an Eu / CuO nanowire as a precursor, finally, the molecularly imprinted membrane assembly material for specifically recognizing PFASs is prepared by taking PFASs as a template molecule through cross-linking polymerization and template desorption, efficient and selective removal of PFASs is realized through a specific capture effect, and the prepared molecularly imprinted membrane assembly material has a good application prospect. The problems that a traditional material is low in adsorption selectivity and insufficient in degradation efficiency are solved, and the composite material is suitable for degrading perfluoroalkyl pollutants in a complex environment, has the advantages of being high in stability, environmentally friendly, capable of being repeatedly used and the like, and has wide market prospects.

The invention relates to the technical field of drug detection, in particular to a molecularly imprinted sensor for quantitatively detecting olanzapine in human blood as well as a preparation method and application of the molecularly imprinted sensor. Au-Pt NSF is deposited on the surface of a substrate electrode through an HBDT method, then a molecularly imprinted polyaniline layer is prepared through electro-polymerization, and the molecularly imprinted sensor is formed. A classical redox probe potassium hexacyanoferrate is used as a tool, and DPV is used for monitoring current change (delta I) caused by olanzapine addition, so that sensitive quantitative detection of olanzapine is realized. The sensor prepared by the invention shows a good linear relationship to olanzapine in the concentration ranges of 0.005-0.4 mu M and 0.4-10 mu M, the detection limit is 0.94 nM, and the sensor has high reproducibility and stability (relative standard deviation RSDlt;

The invention discloses a hydrophilic molecularly imprinted carbon nitride photocatalyst, a preparation method and application of the hydrophilic molecularly imprinted carbon nitride photocatalyst to selective removal of ofloxacin (OFL), and belongs to the technical field of environmental pollution removal. Resorcinol and melamine are used as double hydrophilic monomers, formaldehyde is used as a hydrophilic cross-linking agent, and the hydrophilic molecularly imprinted carbon nitride photocatalyst is prepared. The hydrophilic molecularly imprinted carbon nitride photocatalyst with high selectivity and high activity is prepared by taking carbon nitride prepared by doping p-aminopyridine borate hydrochloride and combining with auxiliary calcination of molten salt as a matrix and OFL as a template and adopting a strategy of cooperation of surface imprinting and a two-step template immobilization method. The OFL in the water environment is stably, efficiently and selectively removed at low cost.