697 results about "Molecular imprinting" patented technology

The invention provides a ratio type molecular imprinting fluorescence sensing detection method based on a metal organic framework and nitrogen-doped carbon dots, the advantages of N-CDs, Eu-MOF and a molecular imprinting layer are fused, in the presence of target molecules, the blue fluorescence (430nm) of the N-CDs is effectively enhanced due to non-radiative recombination caused by the surface defect state of the CDs, and the red fluorescence (660nm) of the Eu-MOF remains unchanged. According to the ratio type molecularly imprinted fluorescent sensing detection method, while a fluorescent response signal is displayed, the color of the imprinted polymer changes along with different concentrations of the target molecules, and sensitive visual detection of the target molecules can be realized, so that specific sensitive detection of the target molecules in a complex sample is realized; the method has the advantages of high sensitivity, simplicity and convenience in operation, good reproducibility, strong anti-interference performance and the like. According to the method, rapid and sensitive detection of organophosphorus pesticides, toxic agents and other imprinted molecules can be realized by preparing imprinted polymers of different target molecules without fluorescence interference, and the method has a wide application prospect.

The invention provides a zeolite imidazate framework fluorescent material and a preparation method and application thereof, and belongs to the technical field of detection material preparation and environment. Quantum dots, a molecular imprinting technology and an MOFs material are combined, firstly, Mg and N co-doped graphene quantum dots and ZIF-8 are compounded, then imprinting is carried out on the surface of ZIF-8, and the zeolite imidazate framework fluorescent material based on MOFs is obtained. In the zeolite imidazate framework fluorescent material, Mg and N co-doped graphene quantum dots not only have good fluorescence performance, but also can generate electron transfer with SDZ to form hydrogen-bond-like acting force, imprinted holes similar to a target object in size and shape are reserved in the surface of the imprinted material, and the target object needing to be detected can be selectively recognized; the zeolite imidazate framework fluorescent material can be used for detecting sulfadiazine in the environment and has good practicability.

The invention relates to the technical field of analysis and detection, in particular to a molecularly imprinted sensor for in-situ detection of epicatechin in plant leaves and a preparation and application method of the molecularly imprinted sensor. The molecular imprinting sensor for detecting epicatechin is prepared by assembling a zeolite imidazate skeleton-amide functionalized single-walled carbon nanotube composite material, amino-formyl imidazole modified reduced graphene and an MIP film on the surface of an electrode layer by layer. The high-selectivity and high-sensitivity detection on the target molecule epicatechin is realized. The technology can effectively reduce the detection cost, simplify the operation process and improve the performance of the sensor. Particularly, due to the introduction of the MIP, the sensor has the capabilities of in-situ detection and high-precision molecular recognition, can realize rapid and reliable quantitative analysis in a complex sample, and has a wide application prospect.

The invention relates to a ratiometric fluorescent molecularly imprinted polymer, a paper-based sensor and preparation and application thereof, and relates to the technical field of food safety detection. The preparation method comprises the following steps: uniformly mixing a functional monomer acrylamide, a template molecule fleroxacin, an initiator, modified SiO2 nanoparticles and a cross-linking agent A to obtain a mixture, then adding a binuclear Eu complex, a boron-nitrogen-doped titanium carbide two-dimensional layered material, a cross-linking agent B and acetic acid, initiating a polymerization reaction, and removing the template molecule to obtain the composite material. The ratio fluorescent molecularly imprinted polymer is obtained. The molecularly imprinted ratiometric fluorescent paper-based sensor can specifically recognize fleroxacin, can be used for detecting fleroxacin residues in meat products, and provides assistance for the development of a food rapid detection technology.

The invention discloses a method for constructing a dual-mode sensor through smartphone-assisted surface molecular imprinting for detecting diphenyl phosphate and application of the dual-mode sensor, and belongs to the technical fields of biochemical analysis, functional nano materials and life science. In synthesis, solubility and selectivity of a target object and photo-initiation efficiency in a polymerization process are optimized, and diphenyl phosphate molecularly imprinted nano-enzyme MIPs is rapidly prepared through a molecular imprinting technology; in the detection, H2O2 and 3, 3, 5, 5-tetramethyl benzidine TMB are catalyzed by peroxidase-like activity of Fe and N-CDs to generate a chromogenic reaction and an inner filter effect of oxTMB is used for inhibiting a fluorescence signal, dual-signal cross validation is realized in cooperation with a smart phone, and the colorimetric-fluorescence sensor for detecting diphenyl phosphate is constructed. The sensor has specific recognition selectivity and binding affinity of diphenyl phosphate, is simple to operate and low in cost, can realize sensitive detection of diphenyl phosphate in a wide linear range, has the detection limit as low as 16 pg / mL, and is suitable for field analysis of trace DPhP in a complex water environment.

The invention discloses a double-template molecularly imprinted composite magnetic covalent organic framework material and a preparation method and application thereof.A layer-by-layer assembly method is adopted, a covalent organic framework is assembled on the surface of aminated ferroferric oxide nanoparticles at the room temperature, then an imprinted layer is formed on the surface of the covalent organic framework through a surface molecular imprinting method, and the double-template molecularly imprinted composite magnetic covalent organic framework material is obtained. And the double-template molecularly imprinted composite magnetic covalent organic framework material MCOF (at) MIP is prepared. The MCOFF-MIP prepared by the preparation method disclosed by the invention has excellent magnetic responsiveness. Experiments show that the MCOFF-MIP has a good adsorption effect and selectivity on four kinds of bisphenol, and has a good application prospect.

The invention constructs a universal visual virus molecularly imprinted sensor modified with an anti-protein coating, the sensor uses magnetic Fe3O4 as an imprinting carrier, HBV as a template and dopamine as a functional monomer for imprinting to obtain a molecularly imprinted polymer with a polydopamine coating (PDA), and before the template is removed, the molecular imprinting polymer with the polydopamine coating (PDA) is separated from the template, so that the molecular imprinting polymer with the polydopamine coating (PDA) is obtained. The surface of the PDA layer is covered with a slightly cross-linked PMEO2MA layer through a water precipitation polymerization method, and the polymer can effectively reduce non-specific protein adsorption; and finally, exposing an imprinting cavity of the PDA layer by eluting the template molecules. On the other hand, potassium permanganate (KMnO4) is used as a signal output molecule, and when no target object exists, the strong reducibility of PDA can enable KMnO4 entering the imprinting cavity to be subjected to a color fading reaction. Based on the principle, high-specificity colorimetric visual detection on the HBV is realized. The sensor can be combined with a smart phone through naked eyes to carry out visual semi-quantitative analysis and carry out quantitative detection through ultraviolet rays, and the universality of the sensor can be confirmed through a template replacement experiment. And meanwhile, the sensor shows an excellent cost advantage. The diagnosis which is high in sensitivity, low in cost and capable of achieving rapid visualization shows huge potential in practical application, and particularly has important application value in infectious disease screening and monitoring work in areas with limited resources.

The invention belongs to the technical field of analytical chemistry detection, and particularly relates to a construction method and application of a tobacco mosaic virus molecular imprinting sensor based on MIL-101 (Fe). The construction method comprises the following steps: by taking a tobacco mosaic virus TMV as a template virus, preparing a molecularly imprinted polymer MIPs with a TMV specific recognition cavity on the surface of MIL-101 (Fe) through a free radical polymerization reaction; the template virus is removed in the preparation process of the molecularly imprinted polymer MIPs, and a TMV specific recognition cavity of the molecularly imprinted polymer MIPs contains MIL-101 (Fe); the molecularly imprinted polymer MIPs is the tobacco mosaic virus molecularly imprinted sensor based on MIL-101 (Fe). Hydrochloric acid is introduced, so that the TMV specific recognition cavity structure of the molecularly imprinted polymer MIPs is cracked to release Fe < 3 + >, then potassium thiocyanate is added, and the Fe < 3 + > and KSCN are subjected to a complexation reaction to generate a red complex. And the complexation reaction of Fe < 3 + > and KSCN is used as a signal probe, the rapid semi-quantitative visual detection of the target virus TMV by naked eyes is realized, and quantitative detection and analysis are carried out through an ultraviolet spectrophotometer.

The invention provides a molecularly imprinted electrochemical sensor for hippuric acid detection, a preparation method and a segmented response technology, and belongs to the technical field of electrochemical sensors. Based on functional monomers aniline and o-phenylenediamine, hippuric acid is taken as a template molecule, and the molecularly imprinted electrochemical sensor for detecting hippuric acid is prepared by an electrochemical polymerization method, and combines the advantages of a molecular imprinting technology and an electrochemical technology; the hippuric acid detection sensor has excellent sensitivity, fast response time and specific recognition capability, has good signal response to hippuric acid detection under different concentrations, generates a good electric signal in a hippuric acid solution of 0-100 nM, forms a better linear relationship between the electric signal generated in the hippuric acid solution with the concentration of 100 nM or above and the concentration, and can be applied to the detection of hippuric acid with the concentration of 100 nM or above. The signal response and detection range of the molecularly imprinted electrochemical sensor under high concentration is widened, and the molecularly imprinted electrochemical sensor is suitable for hippuric acid detection in a wide range.

The invention relates to the technical field of polymer separation and purification, in particular to a preparation method of a molecularly imprinted polymer for separating asiaticoside, and the method comprises the following steps: S1, under an anaerobic condition, sequentially adding asiaticoside and a functional monomer containing unsaturated double bonds and amido bonds into a reactor, and carrying out a reaction for 2-4 hours; carrying out self-assembly reaction on the asiaticoside and the functional monomer to obtain a prepolymer containing an asiaticoside structure and unsaturated double bonds; s2, continuing to add a cross-linking agent and an initiator into the reactor, so that the prepolymer is subjected to a polymerization reaction, and a mixed solution is obtained; s3, separating and filtering to obtain a precipitate, and washing to remove template molecules; and S4, collecting the asiaticoside molecularly imprinted polymer, and carrying out vacuum drying to obtain the asiaticoside-containing molecularly imprinted polymer. According to the present invention, with the molecular imprinting technology, the asiaticoside is specifically recognized by using the functional group so as to embody the specificity and the high selectivity of the method;

The invention discloses a dual-emission molecular imprinting ratiometric fluorescence sensor, a preparation method thereof and application of the dual-emission molecular imprinting ratiometric fluorescence sensor in aureomycin detection, and belongs to the technical field of chemical sensors. The preparation method of the sensor comprises the following steps: preparing silicon dioxide-quantum dot composite nanoparticles, and preparing a molecularly imprinted polymer by taking salicylamide as a template molecule; and mixing the molecularly imprinted polymer and the silicon dioxide-quantum dot composite nanoparticles in an HEPES buffer solution by adopting a post-mixing strategy to construct the dual-emission molecularly imprinted ratiometric fluorescence sensor. The double-emission molecular imprinting ratiometric fluorescence sensor has the beneficial effects that the double-emission molecular imprinting ratiometric fluorescence sensor provided by the invention is rich in fluorescence color change, rapid, high-sensitivity and visual detection of aureomycin can be realized, the whole detection process is simple, convenient and rapid, and the double-emission molecular imprinting ratiometric fluorescence sensor is suitable for being widely popularized in practical application.

The invention belongs to the technical field of organic matter extraction, and particularly relates to a green directional extraction and purification method for haematococcus astaxanthin, which comprises the following steps: (1) mixing dried haematococcus powder and a composite pretreatment agent according to a mass ratio of 1: (8-15), and stirring to obtain a pretreated algae solution; (2) treating the pretreated algae liquid through ultrahigh-pressure microjet equipment to obtain wall-broken slurry; (3) mixing the wall-broken slurry and a composite extraction agent according to a volume ratio of 1: 3-1: 5, and performing extraction separation to obtain an astaxanthin extraction phase; (4) carrying out selective adsorption on the astaxanthin extraction phase through an adsorption column filled with a molecular imprinting adsorbent; (5) collecting eluent by adopting a gradient elution method; and (6) performing vacuum concentration on the eluent, and performing spray drying to obtain an astaxanthin product. According to the method, the astaxanthin extraction efficiency and the product purity are remarkably improved, meanwhile, the energy consumption is reduced to the maximum extent, toxic solvent residues are avoided, and the stability of the astaxanthin is effectively protected.

The present invention discloses the preparation of a fumonisin molecular imprinting polymer and its application in the field of food safety detection. The present invention adopts a double substitution template method to improve the similarity between the substitution template and the target molecule, increase the affinity and selectivity of the molecular imprinting material to fumonisin, and reduce the material preparation cost. Based on the principle of solid phase extraction, a fumonisin molecular imprinting solid phase extraction technology is established to achieve specific recognition, efficient separation and enrichment of fumonisin, and finally realize the detection of fumonisin contamination in agricultural products and feed.

The invention relates to a preparation method and application of an electrochemical sensor for detecting norfloxacin in water, a boron-doped diamond (BDD) electrode is modified by adopting a multi-walled carbon nanotube and gold nanoparticles, and the sensitivity of the electrode is enhanced; and preparing a layer of molecular imprinting film on the surface of a boron-doped diamond (BDD) electrode by taking norfloxacin as an imprinting molecule and adopting an electrochemical in-situ electropolymerization technology, so as to improve the specificity of the electrode and construct an electrochemical sensor for detecting norfloxacin in water. The MIP / AuNPs / MWCNTs / BDD electrode is immersed into norfloxacin buffer solutions with different concentrations for 10 min, and then a differential pulse method DPV is used for testing to obtain a peak current and concentration corresponding relation curve; therefore, the selectivity of the electrode is improved, and the influence of other interfering substances is reduced. Meanwhile, the sensitivity and the stability of the electrode are enhanced, and trace substances are detected.

The invention belongs to the technical field of colorimetric chemical sensors, and particularly relates to a preparation and application method of a smartphone-assisted surface molecular imprinting colorimetric sensor for detecting norfloxacin, magnetic Fe3O4 (at) KH540 nanoparticles are taken as a matrix, magnetic norfloxacin surface molecular imprinting nano-enzyme MMIPs is prepared by adopting a surface molecular imprinting technology, then the MMIPs, H2O2 and 3, 3 ', 5, 6, 7-tetramethyl-3, 5, 6-tetramethyl-3, 5, 6-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3-tetramethyl-3 The colorimetric sensor for detecting norfloxacin is constructed by carrying out a chromogenic reaction on 3, 3 ', 5, 5'-tetramethyl benzidine TMB and cooperating with a smartphone. The sensor has specific recognition selectivity and binding affinity for NOR, is simple to operate, high in detection speed and low in cost, does not need expensive instruments, and can be suitable for field analysis.

The invention discloses a Janus-MOF composite material as well as a preparation method and application thereof. The material is formed by heterogeneously integrating an adsorption module and a signal module through a lattice orientation growth method, wherein the adsorption module adopts a zirconium-oxygen cluster and a tetrafluoroterephthalic acid ligand to construct a molecular imprinting pore channel to specifically capture PFAS; the signal module comprises a spiropyrane modified ligand and an electrochemically deposited gold nano needle tip array, and the SERS detection limit reaches 0.05 ppt through photoresponse aperture regulation and three-mode (electromagnetic-chemical-mechanical) synergistic enhancement; the shell layer is doped with titanium dioxide quantum dots, PFAS can be degraded under ultraviolet irradiation, and the substrate can be recycled for more than or equal to 5 times. The invention further provides a surface enhanced Raman spectroscopy (SERS) and electrochemical impedance spectroscopy (EIS) dual-mode coupling technology, the cross validation detection error is less than or equal to 15%, the problems that the traditional method is poor in selectivity, low in sensitivity and incapable of in-situ regeneration are solved, and the method is suitable for rapid detection of trace PFAS in complex matrixes such as food, surface (undersurface) water and industrial wastewater.

The invention discloses a molecularly imprinted gold nanopore array substrate for single exosome capture-in-situ Raman detection as well as a preparation method and application of the molecularly imprinted gold nanopore array substrate, and belongs to the technical field of biological detection. The molecularly imprinted gold nanopore array substrate structurally comprises a base material and a nanogold layer from bottom to top, the nanogold layer comprises sunken gold nanopores which are distributed in an array mode, the bottoms of the gold nanopores are provided with modified gold nanoparticles, the gold nanopores comprise imprinted cavities matched with exosomes, the size of each gold nanopore is matched with the size of a single exosome, and the size of each imprinted cavity is matched with the size of the corresponding exosome. The gold nanopore channels distributed in the array form a gap between single exosomes, and the gap is greater than or equal to 1 mu m. According to the invention, a gold nanopore channel can be accurately regulated and controlled, and an exosome molecular imprinting structure is modified in the pore channel, so that capture and detection of a single exosome in body fluid are realized; a good adsorption effect on urine exosomes is achieved, and the adsorbed exosomes can be efficiently captured through the nano pore channels; and the nanopore enhancement factor is as high as 7.27 * 10 < 5 >.

The invention discloses a cyclic regeneration treatment method of stainless steel pickling waste liquid, which realizes full-flow recycling of the pickling waste liquid by constructing a closed-loop system of pretreatment, adsorption, desorption, recovery, regeneration and circulation. A molecularly imprinted polymer adsorption technology is adopted, targeted capture of Cr < 3 + >, Ni < 2 + > and Fe < 2 + > / 3 < + > is achieved through specific recognition sites, and the bottleneck that a traditional adsorbent is poor in selectivity is broken through; the supercritical CO fluid extraction technology utilizes the characteristics of high diffusivity and low surface tension, efficient desorption of metal ions is achieved under mild conditions, and secondary pollution caused by a traditional organic solvent is avoided. A technical closed loop is formed among the steps through parameter synergy and substance circulation, and finally the dual purposes of full-amount regeneration of the pickling waste liquid and efficient recovery of metal resources are achieved.

The invention discloses a sialic acid molecular imprinting extraction method, and belongs to the technical field of separation and purification. The invention discloses a sialic acid molecular imprinting extraction method which comprises the following steps: dissolving a sialic acid standard substance and a functional monomer in a binary pore-foaming agent-ionic liquid, adding a cross-linking agent and an initiator, polymerizing under ultraviolet light to prepare a high-molecular polymer, and washing off the sialic acid standard substance to prepare a molecular imprinting polymer; specifically adsorbing sialic acid in the fermentation liquor by using the molecularly imprinted polymer; performing graded elution on sialic acid by using a methanol-water mixed solution and a methanol-acetic acid mixed solution; and performing rotary evaporation concentration, anti-solvent crystallization, separation and drying on the bird's nest acid eluent. Tests show that the sialic acid purity is greater than or equal to 99%, and can meet the requirements of the sialic acid in the fields of food, health care, medicine, cosmetics and the like. According to the method, sialic acid can be efficiently separated and extracted from sialic acid fermentation liquor, the product yield is increased, the extraction procedures are reduced, and the production cost and the discharge amount of three wastes are reduced.

The invention discloses a hydroxyproline rapid detection molecularly imprinted electrochemical sensor as well as preparation and application thereof, and relates to the field of quality safety monitoring of medicinal plants. The preparation method of the molecularly imprinted electrochemical sensor comprises the following steps: preparing the Fc-MWCNTs composite material, preparing the Fc-MWCNTs / GCE, and preparing the molecularly imprinted electrochemical sensor MIP-Fc-MWCNTs (at) GCE. According to the invention, MWCNTs with good conductivity and Fc which has good stability and oxidation-reduction property and is used as an excellent electron transfer medium are used as electrode substrate materials; and the Fc-MWCNTs and the MWCNTs are covalently bonded through an amide reaction to prepare the composite material Fc-MWCNTs which has excellent electron transfer medium characteristics, low potential reproducibility and strong stability and can remarkably improve the electron transfer rate between the electrode surface and an electrolyte. The modified composite material Fc-MWCNTs is modified on the surface of GCE, so that the molecularly imprinted electrochemical sensor MIP-Fc-MWCNTs (at) GCE with high sensitivity, high selective recognition and fast detection is constructed, and the molecularly imprinted electrochemical sensor is used for detecting an index component Hyp of cornu cervi pantotrichum.

The invention discloses a preparation method and application of nano-enzyme molecularly imprinted detection particles, a molecularly imprinted material is developed on the basis of a FeN3P nano-enzyme material, the molecularly imprinted material is fixed on filter paper to realize high-sensitivity and high-selectivity detection of 25-(OH) D3, real-time field detection of the molecularly imprinted material is realized by matching with a self-researched mobile phone APP: Visual Method, and the nano-enzyme molecularly imprinted detection particles have very high practical application value. Accurate data support is provided for early warning and personalized intervention of osteoporosis, community screening and long-term health management lack of 25 (OH) D3 can be promoted, and the method has important scientific value and social significance in reduction of the incidence rate of osteoporosis and optimization of medical resource allocation.

The invention relates to the technical field of quality detection, and provides a method for detecting the quality of an aminobutyric acid extract in peanut sprouts, which comprises the following steps: performing specific recognition on aminobutyric acid by a molecular imprinting sensor, and calculating the impurity contribution by combining a preset peanut sprout interference database and partial least square regression; the interference of complex matrix impurities in the peanut bud extract can be specifically eliminated, and the problem that the specificity is insufficient when a traditional method is used for detecting a complex sample is solved; the molecularly imprinted sensor and the quartz crystal microbalance are connected in series through the microfluidic channel, and resonance frequency detection is triggered by using capacitance variation, so that a coherent process is formed from identification to quality analysis of sample detection, and the detection efficiency is improved; the corrected frequency offset, the phase angle and the temperature are input into the recurrent neural network, accurate output of the concentration of the aminobutyric acid is achieved by integrating multi-dimensional detection information, compared with a single parameter detection method, the real content of the aminobutyric acid in an extract can be reflected more comprehensively, and a reliable basis is provided for quality judgment.

The invention discloses a molecularly imprinted adsorption material taking a metal organic framework as a carrier skeleton as well as a preparation method and application of the molecularly imprinted adsorption material, and belongs to the technical field of adsorption materials. The preparation method comprises the following steps: carrying out hydrothermal reaction on transition metal salt and a phthalic acid ligand to obtain metal organic framework nanoparticles; the preparation method comprises the following steps: adsorbing flavonoid template molecules by metal organic framework nanoparticles, mixing with lysozyme and a disulfide bond reducing agent, performing phase change polymerization, and eluting to remove the flavonoid template molecules after the phase change polymerization is completed, thereby obtaining the molecularly imprinted adsorbing material with abundant imprinting sites and good stability. The adsorbent is good in flavonoid compound adsorption selectivity, high in rate, large in capacity, good in chemical stability, resistant to acid washing, capable of being recycled for multiple times and capable of being widely applied to extraction and separation of flavonoid compounds.

The invention provides a method for detecting chloramphenicol residues in animal food by adopting a molecular imprinting sensor, which comprises the following steps: preparing the molecular imprinting sensor with specific recognition on CAP, and providing an electrochemical detection system of the molecular imprinting sensor for detecting the chloramphenicol residues in the animal food, fitting to obtain a calculation formula of chloramphenicol concentration detected by the molecularly imprinted sensor; treating an animal food sample, and mixing the animal food sample with a standard concentration CAP solution according to a volume ratio of 1: 9 to obtain a to-be-detected sample solution; scanning the to-be-detected sample liquid through the molecular imprinting sensor by adopting a square wave voltammetry method to obtain a peak current value; and obtaining the concentration of chloramphenicol in the sample solution to be detected according to the peak current value and a calculation formula of chloramphenicol concentration detected by the molecular imprinting sensor. The method is sensitive, accurate and practical.

The invention belongs to the field of environment and food pollution detection, and relates to a microcystic toxin-LR (MC-LR) bimodal detection method based on regulation and control of MIP-Cu at CDs laccase activity. Copper chloride dihydrate, citric acid and ethidene diamine are used as synthetic substrates, a fluorescent nano-enzyme with laccase-like activity is synthesized in one step through a hydrothermal method, and the MC-LR can be specifically recognized and the laccase-like activity of MIP-Cu at CDs can be regulated and controlled through wrapping of a molecular imprinting method, so that a colorimetric and fluorescent dual-mode rapid MC-LR determination method is established. The method is short in detection time, high in sensitivity and low in cost. Built-in cross reference correction is provided by dual modes, so that the MC-LR detection reliability and accuracy in a complex environment sample are greatly improved, and false positive output of a detection result is avoided.

The invention relates to a preparation method of a microbial immobilized filter material based on molecularly imprinted biochar. The preparation method comprises the following steps: (1) pyrolysis modification of biochar; (2) molecular imprinting modification and granulation; and (3) carrying out microbial immobilization on the biochar particles. According to the invention, waste straw chippings are used as raw materials, and the molecularly imprinted biochar particles with large specific surface area, rich pore structure, strong selective adsorption, high mechanical strength and excellent impact resistance are prepared through the processes of modification, pyrolysis, molecularly imprinted modification and granulation. Polyvinyl alcohol and sodium alginate are adopted as microbial immobilization materials, and the VOCs degrading bacteria loaded molecularly imprinted biochar particle filter material is formed through chemical crosslinking. The particle size of the filter material is 5-6 mm, the microorganism loading capacity reaches 3-4 mg / g, and the VOCs removal rate reaches 98% or above. The filter material can realize efficient treatment of VOCs, and has the advantages of environmental protection, stable removal effect, strong impact resistance, low operation cost and the like.

The invention discloses a rhizoma polygonati polysaccharide rapid detection electrochemical sensor as well as a preparation method and application thereof, and relates to the field of electrochemical sensors. The method comprises the following steps: (1) preparing an AC-COOH-PANI composite material; (2) preparing an AC-COOH-PANI (at) GCE modified electrode; and (3) preparing the MIP-AC-COOH-PANI (at) GCE. According to the present invention, Glu is adopted as a template molecule, MAA is adopted as a functional monomer, a molecularly imprinted polymer film is constructed on the surface of an AC-COOH-PANI composite material modified GCE electrode through a self-assembly polymerization technology, and the molecularly imprinted electrochemical sensor MIP-AC-COOH-PANI (at) GCE for rapid detection of polygonatum sibiricum polysaccharide is developed, the sensor shows relatively low detection limit, relatively wide linear range, relatively high sensitivity and good selectivity and stability, and has a good application prospect.

The invention relates to the field of biological product preparation, in particular to a preparation method of high-activity oyster-derived small molecule peptide. The method comprises the following steps: selecting fresh meat of pacific oysters or ostrea rivularis as a raw material, degreasing, adding water, crushing, filtering, sequentially carrying out compound protease and neutral protease hydrolysis, implanting a sensor and a probe in hydrolysis, monitoring in real time, and dynamically adjusting reaction conditions; adding a targeting microcapsule wrapping an ascorbic acid glutathione composite protective agent, and releasing the protective agent when a target peptide signal is detected; separating and concentrating through a gradient membrane, and purifying the target peptide by using a molecular imprinting chromatographic column; carrying out alkaline hydrolysis on enzymatic hydrolysis residues to prepare chitosan for cyclic utilization, carrying out enzymatic hydrolysis on residual residues to generate collagen peptide, treating and recycling wastewater, and recovering protease. According to the method, the technical effects that the high-activity and high-purity target peptide is prepared, the resource utilization rate is increased, the production cost is reduced, the target peptide has good oxidation resistance and biological activity, the intestinal enzyme degradation rate is reduced after glycosylation modification, and the blood half-life period is prolonged are achieved.

The invention designs a preparation method of a magnetic molecular imprinting-aptamer sandwich fluorescence sensor for detecting kanamycin. And the magnetic molecular imprinting composite material Fe3O4 (at) UiO-66 (at) MIP with a specific adsorption target object KANA is prepared. When a detection target object KANA exists, the Fe3O4-coated UiO-66-coated MIP can rapidly and specifically capture the KANA in a short time. Then, a DNA single-stranded aptamer marked with FAM is added, after the aptamer and KANA captured on the surface of the Fe3O4 at-UiO-66 at-MIP are subjected to specific recognition and magnetic separation again, an aptamer probe can enter sediment along with the magnetic composite material Fe3O4 at-UiO-66 at-MIP, the fluorescence intensity of supernate is reduced, and quantitative analysis is conducted according to the fluorescence difference value delta F. When the KANA does not exist, the aptamer cannot enter the precipitate, so that the fluorescence signal intensity is not changed. Double recognition is formed through specific binding of an MIP imprinting cavity and the aptamer, matrix interference is reduced by means of fluorescence characteristics and magnetic separation capacity, the method has the advantages of being high in adsorption capacity, specific in recognition, high in sensitivity, wide in detection range, good in selectivity and the like, and rapid and accurate detection of KANA in a complex sample can be achieved.

The invention belongs to the technical field of analytical chemistry, and particularly discloses a magnetic fluorescent gold nanocluster molecularly imprinted polymer and a preparation method and application thereof in sulfadiazine fluorescence detection.The surface of Fe3O4 nanoparticles is coated with a silicon dioxide shell layer, the shell layer is subjected to surface modification, and then the Fe3O4 nanoparticles are combined with gold nanoclusters (AuNCs). On the basis, sulfadiazine (SD) is selected as a template molecule by utilizing a molecular imprinting technology, a mixed type silane coupling agent (APTES) is used as a functional monomer, and the surface of the Fe3O4 (at) SiO2 (at) AuNCs is coated with a molecular imprinting layer, so that the magnetic fluorescent gold nanocluster imprinted polymer Fe3O4 (at) SiO2 (at) AuNCs-MIPs is prepared. By combining a magnetic separation technology, high selectivity of the molecularly imprinted polymer, simplicity, convenience and rapidness and high sensitivity of fluorescence detection, sulfadiazine can be accurately and specifically recognized, and the method can be effectively applied to analysis and detection of sulfadiazine.