46 results about "Molecular imprinting" patented technology

The application provides a composite imprinting polymer and a preparation method and application thereof, and belongs to the field of compound extraction.The composite imprinting polymer comprises a core, a shell and a shell;the core comprises Fe3O4 and SiO2 coated on the Fe3O4;the shell comprises MOF material and imprinting polymer coated on the MOF;raw materials for preparing the imprinting polymer comprise a template molecule, a functional monomer, a crosslinking agent and an initiator;the template molecule is quercetin;the functional monomer comprises two of methyl methacrylate, styrene, 4-vinylpyridine and acrylamide; and the crosslinking agent comprises ethylene glycol dimethacrylate.The material has the triple advantages of magnetic rapid separation, high specific surface area of MOF and specific recognition of molecular imprinting, and exhibits high adsorption capacity and excellent selectivity for quercetin.

The application discloses a temperature-sensitive molecular imprinting sensitive film, a preparation method thereof and application thereof in a glyphosate detection sensor, and belongs to the field of optical sensing technology and environmental monitoring. The application forms an imprinting cavity which is highly complementary to a template molecule in size and spatial configuration in a polymer network, realizes specific identification of a target molecule, and solves the problems of complex sample pretreatment and long detection period of the existing glyphosate detection method. The application introduces a temperature-sensitive material PNIPAM, realizes in-situ, non-destructive and reversible release of the template molecule by temperature control, discards the traditional destructive elution mode, avoids causing serious secondary environmental pollution, avoids the problems of swelling and corrosion of the imprinting cavity caused by strong acid or organic solvent, and causes irreversible damage to the structure of the imprinting cavity, which seriously affects the reuse frequency of the sensor and the detection stability.

The present application belongs to the technical field of adsorbing materials, and particularly relates to a titanium dioxide molecular imprinting polymer as well as a preparation method and application thereof. The present application uses Sudan red as a template molecule, acrylamide as a functional monomer, ethylene glycol dimethacrylate as a crosslinking agent, and azobisisobutyronitrile as an initiator to construct a cavity structure matching the Sudan red molecule on the surface of the amino-modified titanium dioxide ultrafine particles, and successfully prepares the titanium dioxide molecular imprinting polymer. The material combines the photocatalytic characteristics of the titanium dioxide and the selective recognition ability of the molecular imprinting, can degrade the Sudan red under light, and has a higher photocatalytic efficiency than that of the pure titanium dioxide. The material synthesized through the surface imprinting technology has a three-dimensional cavity complementary to the template molecule, and has a larger adsorption capacity than that of the non-imprinted material. The material realizes the integration of the selective recognition, adsorption and degradation of the Sudan red, and is suitable for complex industrial environments.

The application discloses a capillary open-tube chromatographic column preparation method based on a defect engineering metal organic framework-molecular imprinting composite nanomaterial and application, and particularly relates to the field of chemical synthesis and analysis technology. A GLYMO-IDA-silane solution is used to flush a pretreated capillary, so that a carboxyl-modified capillary is obtained. Then, an N,N-dimethylformamide solution containing zirconyl chloride octahydrate, terephthalic acid, L-proline and hydrochloric acid is injected into the cysteine-modified capillary, and under a heating condition, a metal organic framework UiO-66-L-Pro is in-situ grown on the inner wall of the capillary. D-tryptophan and norepinephrine are dissolved in a neutral buffer solution, and after magnetic stirring for 30 min, ferrous sulfate is added and rapidly injected into the UiO-66-L-Pro-modified capillary, and then both ends are sealed, and reaction is carried out at room temperature for 24 h. An acetic acid / SDS solution mixture is used to flush the capillary for 1.5 h to elute the template molecules, and then nitrogen is used to blow dry, so that an MIP@UiO-66-L-Pro@capillary is prepared.

This invention discloses a dual-emission molecularly imprinted ratiometric fluorescence sensor, its preparation method, and its application in the detection of chlortetracycline, belonging to the field of chemical sensor technology. The preparation method of this sensor includes the following steps: preparing silica-quantum dot composite nanoparticles; preparing a molecularly imprinted polymer using salicylamide as a template molecule; and using a post-mixing strategy to mix the molecularly imprinted polymer and silica-quantum dot composite nanoparticles in HEPES buffer to construct the dual-emission molecularly imprinted ratiometric fluorescence sensor. The advantages of this invention are: the dual-emission molecularly imprinted ratiometric fluorescence sensor provided by this invention exhibits rich fluorescence color changes, enabling rapid, highly sensitive, and visual detection of chlortetracycline; and the entire detection process is simple, convenient, and fast, making it suitable for widespread application in practical applications.

This invention relates to the field of infrared spectroscopy detection technology, specifically an in-situ online infrared spectroscopy detection system and method. The system includes: a biomimetic dynamic response material module, used to enhance probe contact pressure through temperature-responsive deformation and specifically capture target molecules through a molecularly imprinted cavity; a quantum-enhanced detection module, composed of a quantum dot infrared enhancement array and an optical phased array spectral scanning module, used to complete spectral acquisition of the molecularly imprinted cavity capture region; an AI-driven closed-loop control module, used to predict reaction paths in real time, optimize temperature / pressure / flow rate parameters, and trigger millisecond-level emergency quenching in case of anomalies; and a self-powered wireless sensor network, used to collect vibrational energy and be self-powered by a supercapacitor, combined with terahertz wireless communication for data transmission. This invention solves the three major bottlenecks of traditional technologies—low sensitivity, high latency, and the need for perforated wiring—through a biomimetic interface quantum-enhanced detection → cableless power transmission → AI millisecond-level decision-making closed loop.

This invention discloses a method for preparing and applying a wearable formaldehyde gas sensor based on molecularly imprinted polymers. The preparation method includes the following steps: forming a heart-shaped electrode stepwise on a flexible substrate using screen printing technology; synthesizing a thin layer of polypyrrole-based molecularly imprinted polymer on the working electrode of the heart-shaped electrode using electropolymerization technology; immersing the synthesized electrode in a mixed solution of acetic acid and methanol; and drying to obtain the wearable formaldehyde gas sensor. This method and application of the wearable formaldehyde gas sensor, through a combination of molecular imprinting technology, screen printing, and electropolymerization, prepares a layered structure of "carbon paste-molecularly imprinted polymer" on a flexible substrate. It can rapidly respond to formaldehyde gas at ppm levels at room temperature, maintains good working condition during multiple bending tests, and exhibits extremely strong selectivity for formaldehyde in complex working environments. The preparation process is simple, low-cost, and highly practical.

The application discloses a preparation method of an antibiotic selective adsorbent, first, C-BN nanosheet balls are prepared by a simple catalytic thermal evaporation method; second, the C-BN nanosheet balls are used as a main body to limit and load magnetic Fe3O4 nanoparticles; and then mesoporous SiO2 and imprinted polymers are coated on the surface of the C-BN nanosheet balls in sequence, and the tetracycline antibiotic selective adsorbent is obtained after template molecules are eluted. The tetracycline antibiotic selective composite adsorbent with high adsorption capacity is prepared, the high specific surface area of the carrier material and the enrichment antibiotic capacity of the radial structure and the selectivity of the surface molecular imprinting material are fully utilized, the selective adsorption of specific antibiotics in a complex solution is effectively realized, and the adsorption efficiency is effectively improved. The carbon-doped BN and the limited loading magnetic particles enhance the adsorption capacity, and the adsorbent can be quickly recovered and separated under an external magnetic field. Meanwhile, the SiO2 layer and the imprinted polymers enable the antibiotics to be quickly desorbed, and the cycle number is more, so that the antibiotic selective adsorbent has application prospects in the fields of sewage treatment and food antibiotic detection.

The application discloses a method for dynamically monitoring microcystin-LR degradation based on a molecular imprinting-carbon nanotube field effect transistor biosensor, which in-situ polymerizes a polydopamine imprinting layer on a CNT-FET channel surface, fully utilizes the significant affinity between polydopamine and MC-LR and the high specific recognition of the molecular imprinting cavity to generate a synergistic effect, and converts the real-time fluctuation of the MC-LR concentration in the degradation process into a channel current signal instantaneously and without damage, so as to realize high-sensitivity, in-situ and bidirectional dynamic monitoring of the whole process of MC-LR degradation kinetics in a complex system.

This invention discloses a molecularly imprinted paper-based microfluidic colorimetric sensor for detecting histamine, its preparation, and its application. The colorimetric sensor (MIP@MIL) is polymerized from a metal-organic framework (MIL-101) using surface molecular imprinting and dual-monomer imprinting techniques. MIP@MIL exhibits a histamine detection limit of 0.77 μM and a detection time of 12.5 min, demonstrating good selectivity. The mechanism involves histamine blocking the imprinted pores of MIP@MIL and acting on MIL-101, thereby weakening the catalytic oxidation process of the chromogenic agent 2,2'-azido-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS). Furthermore, histamine increases the repulsive force of MIP@MIL against ABTS, thus enabling colorimetric detection of histamine. To further improve practical applicability, MIP@MIL was loaded onto qualitative filter paper and further modified onto microfluidic paper-based chips (μPADs). Using a smartphone, rapid and accurate visual detection of histamine in food was achieved, demonstrating its potential for food freshness detection.

The application discloses a kind of molecularly imprinted electrochemical sensor capable of detecting glutamic acid and serotonin simultaneously.The molecularly imprinted electrochemical sensor includes glassy carbon electrode, and (1) amino-functionalized reduced graphene oxide and (2) double-template molecularly imprinted polymer with glutamic acid and serotonin as template molecules and o-phenylenediamine as monomer successively modified on the surface of glassy carbon electrode.Compared with prior art, the molecularly imprinted electrochemical sensor of the application can realize simultaneous detection of non-electrically active glutamic acid and electrically active 5-hydroxytryptamine, thereby saving detection operation and cost, showing good linear relationship and low detection limit for Glu and 5-HT in the concentration range of 1-100 μM, and having good precision, accuracy, selectivity and stability.

The present application relates to the technical field of medical preparations or cosmetics, in particular to the technical field of preparation of active ingredients of plant origin in medical preparations or cosmetics, and discloses a preparation process of centella asiatica extract, which comprises the following steps: adopting enzyme-assisted hot ethanol extraction method, combining with resin purification technology to extract and obtain a centella asiatica concentrated product from centella asiatica; preparing a centella asiatica extract molecular imprinting polymer resin and filling and loading the resin into a solid-phase extraction empty column to prepare a resin column, and purifying the centella asiatica concentrated product through the resin column to prepare the centella asiatica extract with a purity of up to 99%. The prepared centella asiatica extract has the effects of repair and soothing, can be used as an active ingredient in medical preparations or cosmetics, and is used for preparing a gel preparation for treating skin damage, or is added into a repair skin care product to promote skin barrier repair and soothe damaged parts, so that the medical-grade skin care effect and daily care are combined.

This invention provides a method for preparing and applying a LIG molecularly imprinted electrochemical sensor based on the synergistic enhancement of hydrophobic comonomers N-MCz-PABA and AuNPs. Using LIG as the substrate electrode, the invention copolymerizes the hydrophobic monomer N-MCz, the electropolymerizable monomer PABA, and AuNPs precursors onto the electrode surface via a one-step electropolymerization method, forming an N-MCz-PABA@Au molecularly imprinted composite film with both high-affinity recognition sites and high conductivity. This sensor utilizes the enhanced hydrophobic interaction provided by N-MCz to dominate the specific recognition of cortisol, and leverages the in-situ embedded AuNPs to improve interfacial electronic conductivity, combined with the exogenous redox probe K3[Fe(CN)6] to achieve signal transduction. Its detection linear range for cortisol is 0.01–100 μM, with a detection limit of 4.6 nM. This invention features a simple preparation process, low cost, high sensitivity, and good selectivity, providing an effective platform for the immediate and rapid detection of hydrophobic small molecules in complex biological matrices such as sweat.

The invention discloses a titanium-doped zirconium MOF molecularly imprinted electrochemical luminescence sensor for high-sensitivity detection of HER2, and a preparation and detection method thereof, and belongs to the technical field of detection. A titanium-doped zirconium-based metal organic framework (Ti-Zr-TPE MOF) is dispensed on the surface of a working electrode to serve as a luminescent material, Ti-Zr-TPE realizes luminous intensity multiplication in an aggregation state compared with Zr-TPE by further enhancing an intra-molecular motion limited (RIM) mechanism, and a luminous body preparation process, co-reactant screening and detection parameters (such as potential scanning rate and pH value) are systematically optimized, so that the luminous intensity of Ti-Zr-TPE is improved. A set of stable Ti (at) Zr-TPE luminescent system is formed. Meanwhile, dopamine (DA) is used as a functional monomer, and HER2 is subjected to molecular imprinting through a molecular imprinting technology, so that the electrochemical luminescence sensor of a quenching system is constructed. The luminescent material based on the novel tetraphenyl ethylene (TPE) derivative has the advantages of high luminescent intensity, low background signal, high stability and specificity, simplicity and convenience in operation and the like, and has obvious advantages compared with a previously developed sensor for detecting HER2. According to the invention, quantitative detection of HER2 can be realized, and an innovative solution is provided for clinical early warning.

The application discloses a preparation method of a molecular imprinting electrochemical sensor for detecting sodium pentachlorophenol and detection application thereof, and is characterized by comprising the following steps: after ultrasonic dispersion of a sodium pentachlorophenol molecular imprinting polymer suspension, the suspension is drop-coated on the surface of a treated glassy carbon electrode, and the molecular imprinting electrochemical sensor is obtained by standing and drying at room temperature; and a sodium pentachlorophenol detection method is also provided and comprises the following steps: the sensor is placed in a phosphate buffer solution containing Ru(bpy)3 2+ TPrA is polarized, and an initial ECL signal intensity I is recorded; after a to-be-detected PCP-Na solution is added, the ECL intensity I0 is determined by standing and reacting, the ECL signal change value ΔI=I-I0 is taken as a response index, the PCP-Na concentration in the to-be-detected PCP-Na solution is calculated according to the linear relationship between the PCP-Na solution concentration and ΔI, and the method has the advantages of good selectivity and stability, high sensitivity and accuracy.

The present application relates to the technical field of Mongolian medicine external preparation, in particular to a Mongolian medicine Sendeng-4 cream. The cream is prepared from Sendeng, Terminalia chebula, Gardenia jasminoides and Chinese Hopea as raw materials, and effective components are enriched by composite enzymolysis, supercritical CO2 two-phase extraction, gradient membrane separation and molecular imprinting purification. Then, the cream is prepared by adding composite penetration enhancer and plant source preservative after the pH-ROS response carrier targets drug loading and the temperature-sensitive composite matrix is microfluidic adapted. The present application solves the defects of traditional preparation, such as insufficient dissolution, poor stability and weak targeting, and has high cream purity, good skin permeability, excellent safety, stable long-term storage performance, and is suitable for skin inflammation, eczema, itching and other diseases. The cream has the advantages of traditional Mongolian medicine and modern preparation, and has a wide clinical application prospect.

The application provides a molecule imprinting biochar for targeted adsorption of aflatoxin B1 and a preparation method and application thereof, and belongs to the technical field of functional biochar.The application takes oil tea pomace residue after solvent extraction as raw material, first prepares biochar COBC through pyrolysis, then obtains magnetic biochar F-COBC through magnetic modification, then obtains SF-COBC through SiO2 coating, finally takes SF-COBC as a carrier, 5,7-dimethyl coumarin as an aflatoxin B1 simulation template molecule, and performs polymerization reaction on the surface of SF-COBC with the aid of a functional monomer, a crosslinking agent and an initiator, elutes the template molecule, and obtains the molecule imprinting biochar.The molecule imprinting biochar has specific recognition sites for aflatoxin B1, has excellent targeted adsorption performance, and the adsorption amount of the molecule imprinting biochar for aflatoxin B1 can reach 200.02 ng / mg in 80 min, the molecule imprinting biochar is convenient to recover, the adsorption rate can still reach 76% after 5 cycles, and the molecule imprinting biochar can be effectively applied to the adsorption and removal of aflatoxin B1 in agricultural and forestry products, thereby providing a new scheme for the treatment of fungal toxins in the field of food safety.

This paper presents a molecularly imprinted electrochemical sensor for aspartic acid (Asp) and its preparation and detection methods. Belonging to the field of electrochemical analysis and detection, the method involves physically mixing polyethyleneimine (PEI) with multi-walled carbon nanotubes (MXenes) to form a single-layer tubular fiber structure. The MXenes are then bridged by polyethyleneimine to disperse and load the MXenes onto the surface and between the pores of layered MXenes, resulting in a highly conductive MWCNTs-PEI-MXene nanocomposite material that serves as the substrate, enhancing the signal response of the molecularly imprinted sensor. Using o-phenylenediamine as the functional monomer and aspartic acid as the template molecule, a molecularly imprinted electrochemical sensor is constructed, improving the specificity of the electrochemical sensor for Aspartic acid. This preparation method is simple, and the resulting molecularly imprinted electrochemical sensor exhibits good selectivity, reproducibility, and long-term stability, maintaining 92.8% of its initial value even after 7 days of continuous monitoring. This achieves sensitive and specific analysis of Aspartic acid in real samples.

The application relates to the technical field of materials, and provides a molecular imprinting polymer, a preparation method thereof and a method for targeting and enriching phenolic compounds in coal tar creosote. The preparation method of the molecular imprinting polymer comprises the following steps: mixing a template molecule, a main functional monomer, an auxiliary functional monomer, a crosslinking agent, an initiator, a carrier and a pore-forming agent, and performing first stirring; introducing nitrogen into the system, and sequentially performing a pre-polymerization reaction, a main polymerization reaction and post-treatment to obtain a white granular polymer; performing Soxhlet extraction on the white granular polymer by using a mixture of methanol and acetic acid, and then performing vacuum drying to obtain the molecular imprinting polymer. The molecular imprinting polymer provided by the application has a high selective coefficient for phenolic compounds.

This invention provides a flexible multi-gate single-channel OECT molecularly imprinted sensor and its fabrication method, belonging to the field of flexible chemical sensor technology. This sensor employs a unique multi-gate single-channel integrated structure with gates symmetrically arranged at equal intervals around the source and drain electrodes and synergistically controlled within the same channel. Combined with MIP and OECT technologies, it possesses both high-sensitivity amplification capability and highly specific recognition of three non-electroactive BCAAs (leucine, isoleucine, and valine), enabling the detection of multiple substances with a single device.

This invention discloses a dual-recognition electrochemical sensor, its preparation method, and its application. The sensor comprises: (1) a working electrode modified with a molecularly imprinted polymer membrane; and (2) a vancomycin-modified copper-based metal-organic framework nanoenzyme signal probe. This signal probe specifically binds to the cell wall of Gram-positive bacteria and catalyzes the oxidation of o-phenylenediamine by peroxide to generate an electrochemical signal. During detection, the target bacteria are first captured by the molecularly imprinted layer, and the signal probe binds to the bacterial cell via vancomycin, forming a "sandwich" structure. The change in current signal is detected by differential pulse voltammetry to achieve quantitative analysis. The sensor of this invention has a detection limit as low as 1.1 CFU / mL and a linear range of 4 × 10⁻⁶. 1 -4×10 8 The CFU / mL concentration exhibits good selectivity and stability in complex samples such as cerebrospinal fluid. The preparation method of this invention is simple, low-cost, and highly sensitive, and can be used for ultrasensitive detection of Listeria monocytogenes in fields such as central nervous system infections and food safety monitoring.

This invention discloses a 25-(OH)VD3 molecularly imprinted electrochemical sensor based on a three-dimensional dendritic gold nanoassembly, its preparation method, and its application. The preparation method includes: modifying an electrode surface with a three-dimensional dendritic gold nanoassembly to obtain an electrode modified with the three-dimensional dendritic gold nanoassembly; and, using p-phenylenediamine and resorcinol as bifunctional monomers and 25-(OH)VD3 as a template molecule, electropolymerizing the electrode surface modified with the three-dimensional dendritic gold nanoassembly using cyclic voltammetry to form a molecularly imprinted film, followed by removing the template molecule to obtain the 25-(OH)VD3 molecularly imprinted electrochemical sensor. The method for detecting 25-(OH)VD3 using the 25-(OH)VD3 molecularly imprinted electrochemical sensor based on a three-dimensional dendritic gold nanoassembly prepared by this invention has advantages over existing methods such as high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS) and fluorescence methods, including lower cost, easier preparation, convenient operation, and higher sensitivity. Furthermore, this invention provides a new approach and method for detecting abnormal vitamin D metabolism in humans, and has strong practical application.

The application discloses a separation and purification method for preparing pericarpium citri reticulatae essential oil, and relates to the technical field of pericarpium citri reticulatae essential oil preparation.The system comprises the following steps: S1, filtering pericarpium citri reticulatae crude essential oil through microporous filtration to remove solid suspensions and obtain pretreated essential oil; the application first completes impurity removal, dewaxing and preliminary classification of terpenes through three-stage dynamic molecular distillation, reduces the interference of impurities on subsequent adsorption, then realizes specific adsorption of target components through a D-limonene template molecular imprinting column, and combines supercritical CO2 green desorption to realize accurate separation and directional enrichment of pericarpium citri reticulatae essential oil, effectively solves the problems of non-selectivity of traditional distillation, low adsorption efficiency and difficulty in separating structure-similar terpenes, significantly improves the purity of target components, completely removes wax, pigments and isomer impurities, retains the natural activity and aromatic quality of essential oil, the desorption process has no organic solvent residue, the process has strong adaptability, and can meet the differentiated needs of high-end food, medicine and daily chemicals.

This invention belongs to the field of pharmaceutical chemistry and separation analysis, and provides a method for controlling and removing ciprofloxacin hydrochloride dimer impurities. The invention employs a combined design of synergistic control of synthesis and purification process parameters, selective adsorption using molecularly imprinted nanocomposite materials, and reversed-phase chromatographic separation and purification. This achieves a 60-95% enrichment rate of dimer impurity B1 in the crystallization mother liquor, a reference standard purity of 98-99.9%, an online removal rate of 80-99.5%, and a drug loss of only 0.5-5%, while controlling the impurity content in the finished product to no more than 0.05 wt%. This invention solves the problems of existing processes in high-throughput online purification where it is difficult to balance removal rate and bed stability, where there is a conflict between accessibility to recognition sites and structural durability, and where it is difficult to simultaneously optimize deep removal and drug loss control. It has broad application value.

The embodiment of the specification provides an aromatic molecule imprinting electrochemical sensor array, an aromatic molecule detection device, an electronic nose system and application thereof in detection of dangerous solid waste aromatic molecules. A composite film gas sensor array modified by a molecular imprinting polymer and a semiconductor material is used as a detection device, and imprinting cavities capable of selectively adsorbing aromatic molecules exist in the surface film of the device. When different target molecules reach the surface of the molecular imprinting material, the response values of the sensors composed of different materials to the target gas are different, so that the conductive properties of the semiconductor material are changed, and the resistance of the sensor is changed differently. Compared with the current aromatic molecule detection method which is complex to prepare and needs high-temperature conditions, the gas sensor array prepared by the application is small in size, low in manufacturing cost, simple and efficient in detection method and wide in detection range, is a new high-selectivity detection method and has a wide application prospect.

The application relates to an activated sludge-based antibacterial peptide efficient extraction and purification integrated equipment and belongs to the technical field of activated sludge resource recovery. The application provides an activated sludge-based antibacterial peptide efficient extraction and purification integrated equipment, which comprises a pretreatment and impurity removal unit, a low-temperature alkali hydrolysis unit, a temperature control enzyme hydrolysis unit, a primary purification unit, a secondary fractional purification unit, an antibacterial circle online screening unit and a magnetic molecular imprinting nanometer material enrichment and elution unit which are sequentially connected. The application provides an activated sludge-based antibacterial peptide efficient extraction and purification integrated equipment which is stable in process, safe in preservation, suitable for nanometer Fe3O4@MIPs, and capable of continuous operation.

The application discloses a kind of circulating water aquaculture organic matter and antibiotic targeted adsorption degradation method, belong to aquaculture pond water treatment technical field, steps are as follows: the water outlet of mandarin fish culture pond is introduced into microfilter and retained solid phase pollutants;Water outlet enters raw water pool after buffer tank;Raw water pool water outlet enters biological filter and degrades organic matter;Water outlet flows into molecular imprinting targeted adsorption unit, and antibiotic, hormone are captured by double-targeted molecular imprinting polymer adsorbent;Water outlet enters heterogeneous electrofenton catalytic oxidation unit, and electrolysis is synergized with composite catalyst to catalyze and mineralize pollutant;After water outlet is neutralized pool, into air-lift culture tower reoxygenation and culture beneficial bacteria;After sensor monitoring, water quality reaches standard and then backflow aquaculture pond, otherwise backflow to targeted adsorption unit secondary treatment.The application effectively solves the problems that existing mandarin fish culture circulating water treatment process is difficult to target removal terramycin and other pollutants, which causes accumulation, low adsorption and oxidation efficiency, material easy to inactivate, lack of intelligent control, poor water quality adaptability of effluent.

This invention discloses a purification system and method based on a combination of molecularly imprinted microspheres and tubular membranes. The system includes an adsorption reaction unit, a membrane separation and adsorption unit, and an elution and regeneration unit. The method involves the following steps: first, mixing the feed solution with molecularly imprinted microspheres for initial adsorption; then, pumping the slurry into a tubular molecularly imprinted membrane module, where the microspheres are retained, and the membrane simultaneously adsorbs residual target molecules for a second time; after washing, the target product is eluted and collected. This invention achieves nearly 100% adsorption yield through the synergistic effect of microspheres and membranes on the same target molecule, solving the problems of easy clogging of traditional chromatography columns and low capacity of single membranes. It is particularly suitable for the efficient purification of high-value components of natural products.