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Molecularly imprinted magnetic microsphere, preparation method and application thereof

A molecular imprinting and magnetic microsphere technology, applied in the fields of organic chemistry and analytical chemistry, can solve the problems of trace analysis errors, slow elution, and incomplete elution of template molecules in environmental samples, and achieve excellent superparamagnetic properties and excellent selection. sexual effect

Active Publication Date: 2013-03-20
WENZHOU MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] With the continuous expansion of the application of molecular imprinting technology, the disadvantages of the traditional preparation method of MIP have also emerged: part of the imprinted sites are embedded in the polymer body, resulting in incomplete elution of template molecules
These residual template molecules will elute slowly during the experiment, bringing unacceptable errors to the trace analysis of environmental samples

Method used

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  • Molecularly imprinted magnetic microsphere, preparation method and application thereof
  • Molecularly imprinted magnetic microsphere, preparation method and application thereof
  • Molecularly imprinted magnetic microsphere, preparation method and application thereof

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preparation example Construction

[0029] According to a typical embodiment of the present invention, such as figure 2 As shown, the preparation method of molecularly imprinted magnetic microspheres based on alternative templates includes the following steps: S1, the preparation of Fe by co-precipitation 3 o 4 nanoparticles; S2, utilizing Fe by microemulsion polymerization 3 o 4 Nanoparticles to prepare magnetic composite microspheres; S3, using sol-gel method to grow SiO on the surface of magnetic composite microspheres 2 , to obtain core-shell magnetic spheres; S4, grafting C=C double bonds on the surface of core-shell magnetic spheres; S5, performing molecular imprinting operations on the surface of core-shell magnetic spheres grafted with C=C double bonds , to obtain molecularly imprinted magnetic microspheres.

[0030] The present invention prepares Fe by adopting coprecipitation method 3 o 4 nanoparticles, using Fe 3 o 4 Magnetic composite microspheres (nFe 3 o 4 ), and using the sol-gel method...

Embodiment approach

[0031] According to a preferred embodiment of the present invention, utilize coprecipitation method to prepare Fe 3 o 4 The steps of the nanoparticles include: mixing a salt solution containing ferrous ions and ferric ions, heating, and stirring to obtain a mixed solution of iron ions; adding ammonia water and a surfactant to the mixed solution of iron ions, and stirring to obtain Fe 3 o 4 Nanoparticles. Adding a surfactant during the preparation process is to make the prepared nano-scale Fe 3 o 4 Particles are well dispersed and agglomeration is reduced. In the present invention, oleic acid is preferred as the surfactant, but not limited thereto. Further preferably, the salt containing ferrous ions is FeCl 2 4H 2 O, the salt containing ferric ion is FeCl 3 ·6H 2 O.

[0032] Utilization of Fe by microemulsion polymerization 3 o 4 The steps for preparing magnetic composite microspheres from nanoparticles include: Fe 3 o 4 Ultrasonic dispersion of nanoparticles, add...

Embodiment 1

[0043] 1) Synthesis of Fe 3 o 4 Nanoparticles

[0044] Take 6mM (1.24g) FeCl 2 4H 2 O and 12mM (3.24g) FeCl 3 ·6H 2 O was added to 150mL of ultrapure water degassed by ultrasound, vacuumed to remove oxygen with N2, heated to 50°C, and 15mL of NH was quickly added under the condition of stirring at 300rpm and nitrogen. 3 ·H 2 O, add 0.5 mL of oleic acid after reacting for 5 min, and react for 2.5 hours under the conditions of nitrogen flow and stirring. The obtained black oily precipitate was washed three times with absolute ethanol and dispersed in cyclohexane.

[0045] 2) Preparation of nFe 3 o 4

[0046] 1.2g oleic acid modified Fe 3 o 4 Ultrasonic dispersion of nanoparticles in 2mL cyclohexane, adding 4mL styrene and MPS mixture (styrene / MPS=7 / 3, v / v) to form a stable oil phase, ultrasonic 10min; 0.320g dodecyl Sodium sulfonate (SDS), 0.150g Na 2 HPO 4 , 0.096g NaH 2 PO 4 ·H 2 O was dissolved in 120mL of water, and the pH of the aqueous solution was adjust...

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Abstract

The invention discloses a molecularly imprinted magnetic microsphere, a preparation method and an application thereof. The preparation method comprises the following steps: S1, adopting a coprecipitation method to prepare Fe3O4 nanometer particles; S2, adopting a microemulsion polymerization method to prepare a magnetic composite microsphere by virtue of the Fe3O4 nanometer particles; S3, adopting a sol-gel method to grow SiO2 on the surface of the magnetic composite microsphere to obtain a core-shell microsphere; S4, grafting C=C double bond on the surface of the core-shell magnetic microsphere; and S5, adopting a substitution template method on the surface, which is grafted with the C=C double bond, of the core-shell microsphere to carry out molecular imprinting operation to obtain the molecularly imprinted magnetic microsphere. DMIP (Dimethyl Isophthalate) prepared by the method provided by the invention has excellent superparamagnetism and excellent selectivity for target molecules.

Description

technical field [0001] The invention relates to the fields of organic chemistry and analytical chemistry, in particular to a molecularly imprinted magnetic microsphere (DMIP for short) and its preparation method and its application as an adsorbent to selectively identify bisphenol A. Background technique [0002] Molecular imprinting technology is an interdisciplinary technology derived from polymer chemistry, material science, biochemistry and other disciplines. Molecular imprinting technology, also known as molecular template technology, refers to the process of using a specific target molecule as a template to prepare a polymer with specific selectivity for the molecule (ie, molecularly imprinted polymer, MIP). Therefore, it is vividly called "Artificial lock" technology for preparing and identifying "molecular keys". In the 1940s, Nobel laureate Pauling proposed the theory of using antigens as templates to synthesize antibodies, which laid a theoretical foundation for t...

Claims

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Application Information

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IPC IPC(8): C08F290/04C08F226/06C08F222/14C08K3/36C08K3/22C08J9/26B01J20/26B01J20/30B01J20/28C02F1/28
Inventor 林振坤黄长江程文静赫俏雅王力拖李艳艳陈元红戎凯娜
Owner WENZHOU MEDICAL UNIV
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