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Method for preparing magnetic gene-loaded lipid ultrasonic microbubble contrast medium

A technology of ultrasonic microbubbles and contrast agents, applied in echo/ultrasound imaging agents, magnetic therapy, gene therapy, etc., can solve the problem of low imaging, achieve image enhancement, good targeting, and promote apoptosis

Inactive Publication Date: 2011-03-23
SOUTHEAST UNIV
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  • Application Information

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Problems solved by technology

Hepatic metastatic cancer is affected by the pathological type and blood supply of the primary cancer, and its enhancement performance is also different. It can be ring enhancement or rapid mass perfusion enhancement, but the enhanced image usually disappears immediately in the portal phase, and its image is low in liver parenchyma

Method used

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  • Method for preparing magnetic gene-loaded lipid ultrasonic microbubble contrast medium
  • Method for preparing magnetic gene-loaded lipid ultrasonic microbubble contrast medium
  • Method for preparing magnetic gene-loaded lipid ultrasonic microbubble contrast medium

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Embodiment Construction

[0027] (1) Use analytically pure FeCl 3 ·6H 2 O and FeCl 2 4H 2 O was prepared into 0.1mol / L FeCl 3 solution and FeCl 2 solution, by Fe 3+ :Fe 2+ =5:3 add 100ml 0.1mol / L FeCl respectively 3 and 60ml of 0.1mol / L FeCl 2 solution in a flask under N 2 Add dropwise 1.5mol / L of NH under protection and magnetic stirring 4 OH, precipitates appear rapidly in the solution, continue to drop NH 4 OH until the pH of the reaction solution was 9, the precipitate gradually changed from reddish brown to black, and the stirring was continued for 30 minutes before stopping. Mature in a water bath at 80°C for 30 minutes, then hold the bottom of the flask with a strong magnet, pour off the upper layer of waste liquid, rinse the product repeatedly with deionized water until the pH of the solution is 7, and dry the product in vacuum to obtain Fe 3 o 4 magnetic nanoparticles.

[0028] (2) Fe 3 o 4 The dry powder was dissolved in 5g / L oxalic acid solution to make a magnetic fluid with a...

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Abstract

The invention discloses a method for preparing magnetic lipid ultrasonic microbubble contrast medium. The method comprises the following steps: preparing FeCl3 and FeCl2 solution and adding the solutions into a flask; dripping NH4OH under stirring condition so as to generate precipitation and curing; taking out and flushing the generated product until the pH value is 7; drying generated product to obtain the Fe3O4 magnetic nano particles; preparing the Fe3O4 dry powder into magnetic fluid; discarding the supernatant, suspending precipitation again in phosphate buffer and adding PEI to form PEI / Fe3O4 nano particles; mixing the PEI / Fe3O4 nano particles with pEGFP-C1 plasmid DNA for standing to obtain gene-loaded Fe3O4 magnetic nano particles; dissolving DSPC, DPPE and DPPA in an organic solvent in which the ratio of chloroform to isopropanol is 2:1; evaporating the solvent to leave a lipid film; preparing hydration solution from glucose and propylene glycol in a ratio of 4:1 and adding the hydration solution into the flask, and dispersing the hydration solution to obtain lipid suspension; adding 40 mg of PEG4000, heating the mixed suspension for dissolving; adding DNA / PEI / Fe3O4 and gelatin into the lipid suspension; adding 800.01 ml of Tween; replacing air above a penicillin bottle; and shaking the suspension to obtain the gene-loaded magnetic lipid ultrasonic microbubbles.

Description

technical field [0001] The invention relates to a preparation method of a magnetic gene-carrying lipid ultrasonic microbubble contrast agent capable of enhancing images and treating tumors. Background technique [0002] The present invention relates to novel lipid ultrasonic microbubbles and a preparation method thereof. Nanotechnology is introduced into the preparation of ultrasonic microbubbles, and Fe is prepared by nanotechnology. 3 o 4 Magnetic nanoparticles and surface modification of PEI, the modified Fe 3 o 4 Magnetic nanoparticles can be effectively combined with eukaryotic expression plasmids, and then DNA / PEI / Fe 3 o 4 The complex is encapsulated into lipid microbubbles to form gene-loaded magnetic lipid microbubbles. So that it not only has the function of image enhancement but also has the dual anti-tumor cell efficacy of gene therapy and hyperthermia. [0003] Tumor hyperthermia is a method of treating malignant tumors by using physical methods to heat tis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/22A61K48/00A61N2/04A61P35/00
Inventor 张东生李华梅
Owner SOUTHEAST UNIV
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