5467 results about "DNA" patented technology

The present invention relates to the fields of genetic engineering and plant genetic breeding, and specifically to the application of wheat TaPND gene. In the present invention, by taking gene TaPND as a target, designing a sgRNA sequence based on CRISPR / Cas9, connecting a DNA fragment containing the encoding sgRNA sequence to a vector carrying CRISPR / Cas, a plant binary expression vector capable of knocking out the TaPND gene in wheat is constructed; by genetically transforming wheat, offspring with TaPND gene knockout are obtained, and wheat mutants with early flowering time are obtained. Experiments have confirmed that transgenic wheat with TaPND gene knockout achieves the advancement of wheat flowering time. The technical scheme of the present invention provides a practical method for realizing rapid wheat breeding using genetic engineering technology, which has important breeding application value and broad market application prospects.

The invention belongs to the technical field of biology, and provides a porcine epidemic diarrhea virus mRNA vaccine and application. The mRNA molecule comprises a coding region for coding the S protein of the PEDV; the PEDV is of a G2a type, and two proline mutations I1076P and L1077P are introduced into an S protein. The invention further discloses a DNA molecule. An S protein of PEDV; a recombinant plasmid; recombining the host cell; an mRNA vaccine formulation which can be used to prepare an antibody against the S protein of PEDV after immunizing an animal; the invention further discloses application of the mRNA molecule, the DNA molecule, the recombinant plasmid or the host cell to preparation of a medicine for preventing PEDV (Porcine Epidemic Diarrhea Virus) infection. The vaccine prepared by the invention is fast in antibody generation and high in protection rate, can generate high-level neutralizing antibodies in serum and breast milk of sows after the pregnant sows are immunized before parturition, has a remarkable protection effect on piglets, and can effectively prevent the piglets from being infected with PEDV (Porcine Epidemic Diarrhea Virus).

The invention provides an UTR (Untranslated Region) element H2202 P1-G as well as a construction method and application thereof, and relates to the technical field of mRNA (messenger ribonucleic acid). According to the present invention, the ribosome load prediction and the secondary structure optimization are performed on the natural 5 'UTR of the HIV TAT 202 gene through the BaidleHelix platform, and the obtained HTAT 202 P1 sequence avoids the inhibitory hairpin structure so as to significantly improve the luciferase expression quantity compared to the natural UTR; an ncRNA sequence without a secondary structure is introduced on the basis of the HTAT 202 P1, translation inhibition of a 5 'cap region is further relieved, and the protein expression quantity of the constructed H2202 P1-G mutant (the DNA sequence of the H2202 P1-G is as shown in SEQ NO 1, and the RNA sequence is as shown in SEQ NO 2) is further improved.

The invention discloses a genetically engineered algae capable of resisting aquatic pathogenic bacteria as well as a construction method and application thereof, and is characterized in that the genetically engineered algae is phaeodactylum tricornutum into which cell wall energizing plasmids pPha-T1-J48054-eGFP-Linker-Q4 or pPha-T1-J48054-eGFP-Linker-Q6 are introduced, and the preparation method of the genetically engineered algae comprises the following steps: taking genome DNA of the phaeodactylum tricornutum as a template; the method comprises the following steps: constructing a directional expression plasmid pPha-T1-J48054-eGFP by amplifying a Phatr3J48054 coding sequence, inserting Q4 and Q6 coding sequences into the downstream of the eGFP of a directional expression vector, constructing a cell wall energizing plasmid, electrically transforming the cell wall energizing plasmid into phaeodactylum tricornutum, coating an f / 2 plate culture medium with transformed phaeodactylum tricornutum cells for culturing, screening positive algae strains, and screening the positive algae strains. The feed additive has the advantage of inhibiting or killing aquatic pathogenic bacteria.

A system and method for processing genomic data using dynamic latent manifolds that transforms multi-modal genomic datasets into geometric representations within a curved manifold space. The system receives genomic datasets including DNA sequences, genetic variants, and expression data, then extracts biological features and assesses importance using trained neural networks. Manifold curvature values are computed based on biological significance, and genomic data is embedded as geometric structures where semantic relationships are represented through distance and curvature properties. The system generates compression pressure fields that influence processing decisions and computes optimal geodesic paths through the manifold to minimize cognitive action functionals. Adaptive compression rates are determined for different genomic regions based on geometric properties and biological importance. The manifold structure evolves through use, strengthening frequently accessed pathways while applying thermodynamic decay to unused concepts. The system supports hierarchical organization across biological scales, reversible navigation, and federated learning capabilities that enable privacy-preserving collaboration.

Recombinant NK cells, and especially recombinant NK-92 cells express a chimeric antigen receptor (CAR) having an intracellular domain of FcεRIγ. Notably, CAR constructs with an intracellular domain of FcεRIγ had a substantially prolonged duration of expression and significantly extended cytotoxicity over time. The CAR may be expressed from RNA and DNA, preferably as a tricistronic construct that further encodes CD16 and a cytokine to confer autocrine growth support. Advantageously, such constructs also enable high levels of transfection and expression of the recombinant proteins and provide a convenient selection marker to facilitate rapid production of recombinant NK / NK-92 cells.

The invention discloses a self-powered biosensor for double-signal detection of sugarcane pokkah boeng disease, the self-powered biosensor comprises an anode, a cathode and an electrolyte, the anode is a CuCo-MOF / AuNPs / GOD biological anode, the cathode is a CuCo-MOF / AuNPs / DNA chain biosensor, and the electrolyte comprises glucose, MB and a PBS buffer solution. According to the self-powered biosensor, analysis is carried out through two non-interfering modes of electrochemical detection and colorimetric detection, and whether a detection result is a false positive signal or not can be effectively verified. An external power supply is not needed, and real-time outdoor detection can be realized. The material source is wide, the assembly process is simple, and accurate and rapid detection can be realized. The method has the advantages of high sensitivity, good selectivity and real-time detection, and can be used for accurately detecting the sugarcane pokkah boeng rot.

The invention relates to the field of enzyme engineering, in particular to modified xylanase, a DNA (deoxyribonucleic acid) molecule, a recombinant plasmid and a transfected cell. The modified xylanase protected by the application is formed by replacing a deletion segment of a receptor enzyme with a substitution segment, the receptor enzyme is wild type xylanase, the sequence of the receptor enzyme is SEQ ID NO: 1, the deletion segment comprises a carbohydrate binding module of the receptor enzyme, the substitution segment is a carbohydrate binding module of a donor enzyme, the donor enzyme is a carbohydrate active enzyme, and the donor enzyme is a carbohydrate active enzyme. The sequence of the donor enzyme is one of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4 and SEQ ID NO: 5. The carbohydrate binding module of the carbohydrate active enzyme with relatively high activity is replaced into the receptor enzyme, so that the binding capacity of the receptor enzyme and the carbohydrate is improved, and the activity of the receptor enzyme is improved.

The present invention, among other things, provides technologies for detecting and / or quantifying nucleic acids in cells, tissues, organs or organisms. In some embodiments, through sequential barcoding, the present invention provides methods for high-throughput profiling of a large number of targets, such as transcripts and / or DNA loci.

The invention discloses an extraction preparation method and application of algae micromolecule PDRN, and relates to the technical field of biology. The invention establishes a process for extracting PDRN from microalgae, which comprises the following steps: carrying out cell disruption on microalgae liquid, and removing cell debris to obtain filtrate; the filtrate is subjected to DNA dissolution, DNA separation and DNA purification, and a PDRN solution is obtained; wherein the dry weight of the microalgae in the algae liquid is 0.1 to 0.333 g / mL. Through process optimization, the extraction efficiency and purity of the microalgae PDRN are improved, and the extracted PDRN has excellent anti-aging and anti-inflammatory effects.

The invention provides a production strain of an antibiotic-free plasmid, the production strain is a gene editing strain of a PIR strain and is named as PIR1-WN:: 0636 or PIR1-PR: 0636, the production strain contains a nucleotide sequence for coding toxin protein and the antibiotic-free plasmid, and the antibiotic-free plasmid contains a nucleotide sequence for coding antitoxin protein; and preferably, the replicon DNA element of the nonreactive plasmid is R6K-gamma. The toxin protein gene of the production strain disclosed by the invention can be stably passaged, has lethality after being induced and can be used for plasmid screening; according to the invention, the positive rate of transforming the nonreactive plasmid into the PIR1-WN:: 0636 strain is more than 80%, and stable production of the plasmid with a high superhelix ratio can be realized.

The invention relates to the technical field of molecular biology, and particularly provides an InDel molecular marker related to the size of a lychee kernel and application of the InDel molecular marker. The InDel molecular marker is located at a site 25610680 of a fifth chromosome of a reference genome GeneBank: GCA019925255.1 of the litchi, and the InDel molecular marker is used for detecting the DNA of the litchi. The genotype of the marker in a pyrokaryotic litchi material is homozygous' GAAAAA / GAAAAA ', and the genotype of the marker in a macrokaryotic litchi material is heterozygous' G---- / GAAAAA 'or homozygous' G---- / G----'. The invention further provides a primer group for detecting the molecular marker, an effective tool is provided for molecular marker-assisted breeding of the size of the lychee kernel, and the method is expected to assist in shortening the breeding period, improving the breeding efficiency and promoting the improvement work of the lychee variety.

The invention belongs to the technical field of genetic breeding, and relates to an InDel marker for identifying green Chinese onion cytoplasm fertility based on a chloroplast genome and application of the InDel marker. The InDel marker is subjected to amplification detection by adopting a primer group, and the primer group comprises one or more of a first primer group, a second primer group, a third primer group and a fifth primer group; a pair of nucleotide sequences of the first primer group are as shown in SEQ ID NO.1-2; a pair of nucleotide sequences of the second primer group are as shown in SEQ ID NO.3-4; a pair of nucleotide sequences of the third primer group are as shown in SEQ ID NO.5-6; a pair of nucleotide sequences of the fourth primer group are as shown in SEQ ID NO.7-8; and a pair of nucleotide sequences of the fifth primer group are as shown in SEQ ID NO.9 to SEQ ID NO.10. The InDel marker provided by the invention can be used for identifying the cytoplasm fertility of the green Chinese onion, and the tedious procedure and process for extracting DNA can be omitted in the detection process, so that the detection process is simplified.

The invention relates to a nucleic acid molecule of a rice transformation event RN85zN-eH and a detection method. The nucleic acid molecule of the rice RN85zN-eH comprises a sequence shown in SEQ ID NO: 1 or a reverse complementary sequence thereof, or a sequence shown in SEQ ID NO: 2 or a reverse complementary sequence thereof. The rice transformation event RN85zN-eH disclosed by the invention has the characteristics of insect resistance and glufosinate-ammonium herbicide resistance, and the detection method can accurately and quickly identify whether a biological sample contains DNA molecules of the transgenic rice event RN85zN-eH or not.

Rosa chinensis gray mold is a main disease in the cultivation process of rosa chinensis, and seriously threatens the quality and yield of cut-flower rosa chinensis. In order to effectively cope with the disaster stress which influences the yield and quality of the Chinese rose, 192 pairs of SSR primers are used in the research to carry out PCR amplification on DNA of parents' Jinmai '' Beijing red 'and 6 randomly selected offspring. 35 pairs of primers show genetic difference between male and female parents. The 35 pairs of primers are further used for carrying out PCR amplification on filial generation DNA, detection is carried out through PAGE electrophoresis, and an electrophoretic band is converted into a 0, 1 matrix for genetic typing. The method comprises the following steps: selecting an optimal subset through correlation regression analysis, and finally successfully screening out three primers, namely, Geno-BC-1, Geno-BC-2 and Geno-BC-3, which are related to gray mold resistance. The discovery provides an important theoretical basis for molecular marker-assisted Chinese rose disease-resistant breeding, and is expected to promote early identification of disease-resistant plants, so that the breeding period is shortened, and the breeding efficiency is improved.

Compositions and methods for delivering nucleic acids (e.g. DNA) to cells are described herein. In some embodiments, the compositions and methods involve the use of lipid nanoparticles comprising cationic lipids, helper lipids, conjugated (PEG) lipids and structural (sterol) lipids wherein the helper lipid has at least one acyl chain bearing an unsaturation which improves in vivo delivery of the nucleic acid pay load.

The invention relates to the technical field of deep-sea microorganism nucleic acid detection, in particular to a long-term experimental platform for deep-sea microorganism in-situ nucleic acid detection and a detection method. According to the invention, a water sample is subjected to tangential flow filtration and concentration through the filtration and sampling module, so that a concentrated microorganism sample is obtained. The method comprises the following steps of: transferring a sample into an amplification detection module under reduced pressure, transferring into a lysis purification module for cell lysis, injecting the cracked sample into a chromatographic column, filtering impurities and intercepting DNA by the chromatographic column, then washing by using an eluent to obtain a high-purity DNA sample, loading a suction head through a transfer washing liquid gun, mixing the high-purity DNA sample with an enzyme solution, adding into the amplification detection module for reaction, and detecting the DNA in the amplification detection module. And carrying out fluorescence labeling on the amplified gene, obtaining an amplification curve by using a fluorescence detector, and detecting the in-situ nucleic acid of the deep-sea microorganisms according to the change of the amplification curve. An automatic cleaning assembly is designed in the filtering and sampling module, and the cracking and purifying module is used for sampling and cleaning a cracking chamber and switching pipelines, so that cross contamination caused by deep sea in-situ multiple detection is prevented.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to growth traits of litopenaeus vannamei and application of the SNP molecular marker, and belongs to the field of molecular marker-assisted breeding of aquatic animals. The genome DNA of the muscular tissue of the litopenaeus vannamei to be detected is extracted as a template, the gene is subjected to PCR amplification and sequencing, the genotype of the molecular marker SNP1-3 is determined, and the molecular marker SNP1-3 is used as the SNP molecular marker for seed selection, so that the aim of quickly screening litopenaeus vannamei individuals with obvious growth advantages in the early stage is fulfilled. According to the molecular marker related to the growth traits of the litopenaeus vannamei, the breeding period can be greatly shortened, the fine breed breeding process of the litopenaeus vannamei is accelerated, the breeding efficiency is improved, and the breeding cost is reduced.

The invention provides a grass carp sourced U6 promoter. The sequence of the grass carp sourced U6 promoter is shown as one of SEQ ID NO: 1-3. Three grass carp-derived U6 promoters are cloned, the transcriptional activity and cross-species adaptability of the three promoters are analyzed, results show that the three promoters can show transcriptional activity in fish cells and human cells, the transcriptional activity of ciU6-1 is remarkably higher than that of the other two promoters, heterologous DNA can be transcribed in various cells, and the expression of the ciU6-1 in the fish cells and the human cells is improved. And the method can be used for gene editing means based on Cas9.

The invention belongs to the technical field of biological detection, and particularly relates to a disease marker fluorescence instant detection method based on protein scaffold DNA-Cu2 + functional nanospheres, which comprises the following steps: combining an insulin-like growth factor 1 (IGF-1) aptamer or cortisol aptamer, protein-DNA and DNA-metal ions; the protein scaffold DNA-Cu < 2 + > multifunctional integrated nanospheres are synthesized by a one-step method at room temperature. Based on the function integration of the nanosphere, one-step detection of IGF-1 or cortisol is realized, a plurality of analysis modes including fluorescence detection and smartphone-RGB are provided by combining a test strip and smartphone red, green and blue (RGB) analysis technologies, and a rapid and sensitive disease marker instant detection method is constructed. Finally, rapid and convenient detection of multiple markers in hundreds of clinical samples of multiple diseases is realized, and a new solution is provided for paper-based fluorescence POCT.

The invention relates to the technical field of teasel breeding, and discloses a teasel germplasm resource multi-character collaborative screening breeding method, which comprises the following steps: multi-omics data acquisition: extracting teasel germplasm leaf DNA (deoxyribonucleic acid), and capturing exon regions of disease resistance and metabolism related genes by adopting a targeted sequencing technology to obtain an SNP (single nucleotide polymorphism) marker matrix; the method comprises the following steps: collecting root extracts, detecting medicinal components through ultra-high performance liquid chromatography-mass spectrometry, and constructing a metabolite abundance matrix; measuring phenotype data of plant height, root length, root weight, root rot morbidity and survival rate under drought stress; key site and metabolite correlation analysis: constructing a Bayesian network containing SNP markers, metabolite and phenotypes by using a graph database, and carrying out cross validation through a leave-one-out method. The dipsacus asper germplasm resource multi-character collaborative screening breeding method aims at solving the problem that a traditional breeding method lacks systematic integration analysis of genomes and metabolic pathways in dipsacus asper multi-character collaborative improvement.

The invention provides a method for detecting influence of microplastics and antibiotics on a sediment microbial community structure and an ecological function. The method comprises the following steps: acquiring a sediment sample at a sampling point; adding the micro-plastic particles and the antibiotic solution into the sediment sample, and incubating; dNA of the microorganisms is extracted, and 16S rRNA sequencing is carried out; and on the basis of a sequencing result, microbial community diversity and characteristic bacterial class group identification and ecological function evaluation are carried out. According to the invention, the comprehensive effect of micro-plastic and antibiotic combined pollution on the structure and ecological function of the microbial community in the sediment is comprehensively analyzed for the first time, and the detection speed and accuracy of the microbial community in the sediment are improved by applying a 16S rRNA detection technology; and a solid support is provided for formulating an environment protection strategy, optimizing pollution control and implementing an ecological restoration project.

Provided herein are technologies for framing and evaluating biological sequence-based analysis tasks in a unified, natural-language-based, text in and text out format. Among other things, methods and systems of the present disclosure provide machine-learning technologies for combining biological sequence data, representing, for example, DNA, RNA, and protein sequences, with natural language, conversational style prompts that set out particular analysis tasks to be performed on the biological sequence data. This approach, for example, allows complex analysis tasks, including, but not limited to, identification of various sequence modifications, genes, and regulatory elements in DNA sequences, and quantification of properties such as degradation propensity of RNA and protein stability, to be input to a machine learning model in a uniform text-based format and for output to be generated in a same, unified, text-based format.

The present disclosure provides compositions, apparatus and methods for generating a plurality of concatemer template molecules immobilized on a support for conducting massively parallel sequencing runs. In some embodiments, the concatemer template molecules can be generated by conducting rolling circle amplification reactions on a support comprising a mixture of immobilized capture and pinning primers. The rolling circle amplification reaction comprises a plurality of circularized polynucleotide molecules and soluble amplification primers which generates concatemer template molecules that collapse to form compact DNA nanoballs that are stably immobilized to a support.

The invention provides a homogeneous electrochemical analysis method for exosome detection based on DNA-Pb < 2 + > functional nano-hydrogel and application, and relates to the technical field of biomedical diagnostic analysis method.The homogeneous electrochemical analysis method is constructed based on DNA nano-hydrogel prepared through a rolling circle amplification (RCA) method by combining a G-quadruplex structure and a Pb < 2 + > specific recognition mechanism; an epithelial cell adhesion molecule (EpCAM) on the surface of the exosome is selected as a detection target, the DNA nano-hydrogel is triggered to disintegrate by utilizing specific binding between the EpCAM and an aptamer thereof, then a large number of G-quadruplex structures generated by RCA are exposed, the G-quadruplex structures can be selectively bound with Pb < 2 + > and regulate and control electrochemical signal output, and the detection sensitivity is high. According to the method, a one-pot method, a rapid and efficient detection process is realized, and the whole process is completed within 45 minutes.

Disclosed are lipid nanoparticle (LNP) delivery systems that specifically target T cells. The LNP delivery system comprises antibodies conjugated to the surface of the LNP, e.g., via maleimide chemistry, that target at least two T cell surface proteins, e.g., CD3 and CD28. The LNP delivery system can have a single population of LNP conjugated to either a bispecific antiCD3 / antiCD28 antibody, or two monospecific antiCD3 and antiCD28 antibodies, or two populations of LNP wherein each population comprises a monospecific antibody. The payload of the LNP delivery system can be, e.g., mRNA encoding chimeric antigen receptors (CAR), a linear DNA fragment or a plasmid encoding chimeric antigen receptors (CAR) or therapeutic proteins such as antibodies, components of a gene editing systems (e.g., CRISPR-Cas), small molecules, antibody-drug conjugates (ADC), and any combination thereof, either encapsulated in the LNP or attached to its surface (e.g., conjugated). Also provided are lipids, pharmaceutical compositions, kits, and methods of treatment.

The present disclosure relates to compositions and methods for reducing expression of MYC gene in a cell. In some embodiments, an expression repressor comprises a targeting moiety that binds a MYC promoter, anchor sequence, or super-enhancer. In some embodiments, the expression repressor comprises an effector moiety that represses transcription or methylates DNA. Systems comprising two expression repressors are also disclosed. The compositions can be used, for example, to treat cancers such as HCC.

The invention discloses a method for monitoring diversity of large benthic invertebrates in a river based on eDNA. The method comprises the following steps: step 1, selecting a river basin to be measured, and querying basic information of the basin; 2, collection and treatment of eDNA samples: designing sampling points and geographic positions, and collection and pretreatment of water samples; 3, measuring physical and chemical indexes; 4, extraction of eDNA, PCR amplification and high-throughput sequencing are carried out; and 5, performing data analysis on the results, wherein the data analysis comprises bioinformatics analysis, alpha diversity analysis, beta diversity analysis and correlation analysis of large benthic invertebrate communities and environmental factors. The diversity of the large benthic invertebrates is more accurately and efficiently investigated and monitored by analyzing DNA fragments existing in the environment.

The invention relates to the technical field of biosensing, in particular to a method for synergistically activating a CRISPR / Cas12a system based on a T-type structure and application of the method, the CRISPR / Cas12a system can be activated by utilizing two split DNA activation chains and crRNA to form the T-type structure, the activated CRISPR / Cas12a system has trans-cleavage activity, an FQ-ssDNA probe modified with an FAM fluorophore and a BHQ1 quenching group can be subjected to trans-cleavage, a fluorescence signal is recovered, and the fluorescence signal is recovered. Through reasonable design of sources of activation chains corresponding to different targets, detection targets can be expanded from traditional nucleic acid detection to non-nucleic acid targets such as proteins and metal ions, so that biosensing application of multiple targets is realized, in addition, design based on a T-type CRISPR / Cas12a synergistic activation strategy has great flexibility and universality, and the application prospect is wide. The method can be used together with various amplification strategies and detection platforms, and the sensitivity of the analysis method is effectively improved.

The invention relates to the technical field of biology, in particular to an anti-pollution freeze-dried eight-color Mini-STR composite amplification kit. Through design and optimization, the primer group, the reagent and the kit for detecting the eight-color multiple fluorescence labeling Mini-STR capable of identifying 25 STR gene loci are developed; the primer group, the reagent and the kit are used for detecting forensic samples, the detection sites are more, the detection accuracy is higher, the anti-pollution capacity is high, the detection capacity on trace samples is higher, the detection rate of degraded sample DNA can be remarkably increased, and the kit is higher in stability and has wide application value.