3221 results about "DNA" patented technology

The invention provides an UTR (Untranslated Region) element H2202 P1-G as well as a construction method and application thereof, and relates to the technical field of mRNA (messenger ribonucleic acid). According to the present invention, the ribosome load prediction and the secondary structure optimization are performed on the natural 5 'UTR of the HIV TAT 202 gene through the BaidleHelix platform, and the obtained HTAT 202 P1 sequence avoids the inhibitory hairpin structure so as to significantly improve the luciferase expression quantity compared to the natural UTR; an ncRNA sequence without a secondary structure is introduced on the basis of the HTAT 202 P1, translation inhibition of a 5 'cap region is further relieved, and the protein expression quantity of the constructed H2202 P1-G mutant (the DNA sequence of the H2202 P1-G is as shown in SEQ NO 1, and the RNA sequence is as shown in SEQ NO 2) is further improved.

A system and method for processing genomic data using dynamic latent manifolds that transforms multi-modal genomic datasets into geometric representations within a curved manifold space. The system receives genomic datasets including DNA sequences, genetic variants, and expression data, then extracts biological features and assesses importance using trained neural networks. Manifold curvature values are computed based on biological significance, and genomic data is embedded as geometric structures where semantic relationships are represented through distance and curvature properties. The system generates compression pressure fields that influence processing decisions and computes optimal geodesic paths through the manifold to minimize cognitive action functionals. Adaptive compression rates are determined for different genomic regions based on geometric properties and biological importance. The manifold structure evolves through use, strengthening frequently accessed pathways while applying thermodynamic decay to unused concepts. The system supports hierarchical organization across biological scales, reversible navigation, and federated learning capabilities that enable privacy-preserving collaboration.

Recombinant NK cells, and especially recombinant NK-92 cells express a chimeric antigen receptor (CAR) having an intracellular domain of FcεRIγ. Notably, CAR constructs with an intracellular domain of FcεRIγ had a substantially prolonged duration of expression and significantly extended cytotoxicity over time. The CAR may be expressed from RNA and DNA, preferably as a tricistronic construct that further encodes CD16 and a cytokine to confer autocrine growth support. Advantageously, such constructs also enable high levels of transfection and expression of the recombinant proteins and provide a convenient selection marker to facilitate rapid production of recombinant NK / NK-92 cells.

The invention discloses an extraction preparation method and application of algae micromolecule PDRN, and relates to the technical field of biology. The invention establishes a process for extracting PDRN from microalgae, which comprises the following steps: carrying out cell disruption on microalgae liquid, and removing cell debris to obtain filtrate; the filtrate is subjected to DNA dissolution, DNA separation and DNA purification, and a PDRN solution is obtained; wherein the dry weight of the microalgae in the algae liquid is 0.1 to 0.333 g / mL. Through process optimization, the extraction efficiency and purity of the microalgae PDRN are improved, and the extracted PDRN has excellent anti-aging and anti-inflammatory effects.

The invention provides a production strain of an antibiotic-free plasmid, the production strain is a gene editing strain of a PIR strain and is named as PIR1-WN:: 0636 or PIR1-PR: 0636, the production strain contains a nucleotide sequence for coding toxin protein and the antibiotic-free plasmid, and the antibiotic-free plasmid contains a nucleotide sequence for coding antitoxin protein; and preferably, the replicon DNA element of the nonreactive plasmid is R6K-gamma. The toxin protein gene of the production strain disclosed by the invention can be stably passaged, has lethality after being induced and can be used for plasmid screening; according to the invention, the positive rate of transforming the nonreactive plasmid into the PIR1-WN:: 0636 strain is more than 80%, and stable production of the plasmid with a high superhelix ratio can be realized.

The present disclosure provides compositions, apparatus and methods for generating a plurality of concatemer template molecules immobilized on a support for conducting massively parallel sequencing runs. In some embodiments, the concatemer template molecules can be generated by conducting rolling circle amplification reactions on a support comprising a mixture of immobilized capture and pinning primers. The rolling circle amplification reaction comprises a plurality of circularized polynucleotide molecules and soluble amplification primers which generates concatemer template molecules that collapse to form compact DNA nanoballs that are stably immobilized to a support.

The present disclosure relates to compositions and methods for reducing expression of MYC gene in a cell. In some embodiments, an expression repressor comprises a targeting moiety that binds a MYC promoter, anchor sequence, or super-enhancer. In some embodiments, the expression repressor comprises an effector moiety that represses transcription or methylates DNA. Systems comprising two expression repressors are also disclosed. The compositions can be used, for example, to treat cancers such as HCC.

Described herein are programmable DNA proteolytic target chimera complexes that can be used both for the direct treatment of cancer by inhibiting biochemical pathways that are overexpressed in cancer cells, and for the indirect treatment of cancer by recruiting the E3 ligase complex to engage with a protein of interest or a mutant thereof and initiating proteolysis. Also described herein are methods of using the complexes in the treatment of cancer, as well as compositions comprising the complexes.

The invention discloses application of a gene marker in early screening of esophagus, stomach and intestine multiple cancer species, an early screening model construction method and a detection device, and belongs to the technical field of early noninvasive detection of digestive tract tumors. By analyzing the whole genome characteristics of circulating free DNA in peripheral blood, a novel multi-cancer-species screening system is established. On the basis of low-depth whole genome sequencing data, molecular markers in three dimensions, namely a genome copy number variation mode, a DNA fragment distribution characteristic with a specific length and an epigenetics signal of a transcription initiation region, are emphatically detected. An advanced converter neural network architecture is adopted, and the model can efficiently capture complex feature association in a whole genome range through a specific self-attention mechanism. The model design particularly considers the particularity of genome data, introduces an adaptive position coding system, and accurately reflects the spatial distribution relationship of DNA fragments on chromosomes. Therefore, the system can still maintain excellent detection performance under extremely low sequencing depth.

Disclosed in the present invention are an HLA-A*11-targeted liver cancer mRNA vaccine, and a preparation method therefor and a use thereof. The mRNA vaccine of the present invention is an mRNA vaccine designed on the basis of the HLA-A*11:01 typing of a patient and having high-coverage and high-immunogenic tumor neoantigens, and is formed by transcribing DNA having a nucleotide sequence shown in SEQ ID NO: 32 to form an mRNA, and encapsulating the mRNA in lipid nanoparticles.

The invention relates to the technical field of molecular markers, in particular to an Indel molecular marker related to yellow leaves of a melon seedling growing point and application of the Indel molecular marker. The invention explores a key candidate gene MELO3C016136 for controlling the leaf color of the growth point of the muskmelon seedling, and develops an Indel molecular marker ygp2 for identifying the leaf color change of the growth point of the muskmelon seedling based on the key candidate gene MELO3C016136. The molecular marker ygp2 can be used for identifying the leaf color change character of the growth point of the muskmelon seedling, only one-time PCR amplification and polyacrylamide gel electrophoresis need to be carried out on the DNA of the plant to be detected, the identification result is reliable, the genotype of the target plant can be simply and conveniently identified, and the genotype identification result is completely consistent with the leaf color phenotype of the growth point of the seedling; important technical support is provided for creating new muskmelon germplasm with yellow seedling growing point leaves.

Provided herein are certain fused ring compounds, such as a compound of Formula (A), as Werner Syndrome RecQ DNA helicase (WRN) inhibitors, pharmaceutical compositions comprising the compounds, and method of use of the compounds or pharmaceutical compositions in the treatment of diseases or disorders.

The invention relates to the technical field of biological medicines, and particularly discloses a preparation method and application of intestinal tract targeted DNA (deoxyribonucleic acid) nano origame.The preparation method is technically characterized by comprising the following steps: S1, synthesizing planar DNA nano origametes (DONs); s2, assembling the DONs and the siRNA (small interfering ribonucleic acid); and S3, synthesizing the tubular DNA nano origami loaded with siRNA: uniformly mixing the purified DONs-siRNA origami with 10 times of excessive APE1 chain and cross-linked chain, annealing from 45 DEG C to 25 DEG C, and synthesizing the tubular DNA nano origami. According to the method disclosed by the invention, a nano delivery carrier which has intestinal targeting, is accurately loaded and can release siRNA as required is developed by utilizing a DNA (Deoxyribonucleic Acid) nano technology, so that efficient treatment of IBD (Infectious Bursal Disease) by siRNA is realized.

The invention belongs to the technical field of essential gene prediction, and particularly relates to an essential gene prediction method based on DNA large model and time-frequency domain deep learning fusion, and the method comprises the steps: taking a domain DNA large model as a core representation layer, and obtaining special gene representation through cross-species corpus pre-training and task fine tuning; a T-Block and F-Block dual-channel time-frequency fusion structure is adopted, and the local dependence and long-range regulation relation of a gene sequence is synchronously captured by expanding DFT (Discrete Fourier Transform), complex value attention and iDFT (Initial Discrete Fourier Transform) conversion; designing an efficient modeling reasoning scheme of sliding window slices and gene-level aggregation aiming at an ultra-long sequence; in combination with class imbalance and a noise robust training strategy, cross-cell line / cross-platform transferable threshold output is realized through temperature scaling calibration, an uncertainty quantization and structured interface is matched, and drug target screening and experimental design decision are supported. The system supports the realization of multiple programming languages, and can complete low-delay end-to-end reasoning in a conventional hardware environment.

Disclosed are a genomic mating method for Huaxi cattle based on whole genome single nucleotide polymorphism (SNP) information and an application thereof. The method includes the following specific steps: step 1, extracting deoxyribonucleic acid (DNA) from to-be-hybridized Huaxi cattle individuals for genotyping; step 2, performing genotype data imputation to obtain high-density chip data; step 3, calculating an additive genetic relationship matrix, utilizing genomic best linear unbiased prediction (GBLUP) to obtain genomic estimated breeding values of five important economic traits of a to-be-hybridized Huaxi cattle population, and calculating a comprehensive selection index of the individuals; and step 4, using a genetic algorithm to construct a population optimal mating combination list. In the present invention, the breeding cost is greatly saved and an inbreeding level of offspring populations is reduced.

This disclosure generally relates to methods and compositions for eliciting broad and robust immune responses to a protein of interest. The methods employ both DNA and RNA-based vaccines that encode at least a portion of the protein of interest.

The invention relates to the technical field of microbiological detection and molecular biology, in particular to a specific detection primer for bifidobacterium longum subsp. Longum BBMN68 and application of the specific detection primer. The specific primer for detecting the bifidobacterium longum subsp.longum BBMN68, provided by the invention, comprises an upstream primer and a downstream primer, and nucleotide sequences are shown as SEQ ID NO.7-8. The invention also provides a kit for detecting the bifidobacterium longum subsp.longum BBMN68. The specific DNA fragment provided by the invention is a specific molecular marker of BBMN68, and the specific primer is designed aiming at the fragment sequence, so that the specific amplification of the strain can be realized, no cross reaction is caused to other related strains, the detection sensitivity is higher, and the detection requirements of samples with different concentrations can be met; the method can be used for specific detection and accurate quantification of the BBMN68 strain in yoghourt and other products, and has good practical application value.

The invention relates to a regenerated decellularized adipose derivative as well as a preparation method and application thereof. The preparation raw material of the regenerated decellularized adipose derivative is prepared from a vascular matrix component (SVF) separated from human adipose tissue through decellularization treatment and freeze drying; the prepared regenerated decellularized adipose derivative is a porous material, the porosity is greater than 90%, and the aperture is 50-200 [mu] m; the composition comprises the following components in percentage by weight: 60-70% of collagen, 15-20% of elastin, 1 * 10 < 10 >-5 * 10 < 10 > particles / g of exosome, less than or equal to 5 ng / mg of DNA (deoxyribonucleic acid) residue, more than or equal to 30 ng / g of VEGF (vascular endothelial growth factor) and more than or equal to 15 ng / g of FGF-2 (fibroblast growth factor). The prepared regenerated decellularized adipose derivative is low in DNA residual quantity, rich in growth factors and capable of meeting the requirement for deep tissue filling due to the fact that the collagen content and the elastin content are high.

The invention relates to the technical field of electromagnetic metasurfaces and bionic computing, and discloses a bionic information processing method and system based on an electromagnetic metasurface. The method comprises the following steps: S1, mapping DNA sequence information into a multi-dimensional coding state of the bionic metasurface information processing unit in an electromagnetic adjustable parameter space; s2, realizing co-evolution of the multi-dimensional coding state in space, frequency, phase and amplitude dimensions by dynamically regulating and controlling electromagnetic response parameters of the bionic metasurface information processing unit; and S3, using propagation and interference of electromagnetic waves in the diffraction neural network and a near-field coupling effect between the bionic metasurface information processing units to complete copying, logical operation and information read-write operation of the DNA sequence information. The functions of DNA coding, copying, logic calculation, information reading and writing and the like are uniformly mapped into the programmable electromagnetic metasurface platform, and cooperative processing of information in the aspects of space, electromagnetism and algorithm is achieved.

A method for performing local alignment based on a query sequence of DNA and a reference sequence of DNA includes: obtaining a bit matrix H; determining at least one diagonal based on the bit matrix H; for each of the at least one diagonal, calculating an initial score for the diagonal, determining at least one trace region, determining a sub-alignment for each of the at least one trace region, consolidating the diagonal and the sub-alignment respectively of the at least one trace region to obtain an alignment, and obtaining an alignment score based on the initial score and the partial score respectively of the sub-alignment respectively of the at least one trace region; and among each of the at least one alignment thus determined respectively for each of the at least one diagonal, reserving one of the at least one alignment that has the highest alignment score therefrom.

The invention provides a nucleotide mutation site prediction model construction and disease-related point mutation identification method. Specifically, the invention provides a deep learning model-fused nucleotide mutation site prediction model construction method and a disease-related point mutation identification method. According to the method, DNA point mutation and RNA point mutation can be recognized from transcriptome sequencing data in a high-sensitivity and high-specificity mode, and basic data is provided for explaining mutation generation mechanisms and functions on the whole transcriptome and genome level.

The application provides an in-vitro cell-free protein synthesis system and kit containing exogenous magnesium ions and application thereof, including a D2P system (DNA-to-Protein system) and an mR2P system (mRNA-to-Protein system), and belongs to the technical field of protein synthesis. The in-vitro cell-free protein synthesis system containing exogenous magnesium ions adopts magnesium aspartate as a novel magnesium ion source, and especially in a eukaryotic cell-free system, compared with a traditional magnesium ion source, can significantly improve the protein synthesis efficiency and protein expression amount. A more efficient and higher-throughput in-vitro protein synthesis kit and a synthesis method of exogenous proteins are also provided, and the kit has the advantages of simplicity, convenience and low cost.

The utility model provides a combined reagent kit for DNA (deoxyribonucleic acid) purification experiment, which comprises a box body, a damping rotating shaft is arranged on the bottom wall of the box body, the bottom wall of the box body is rotatably connected with a classified storage disc through the damping rotating shaft, and a driving component for driving the classified storage disc to rotate is arranged at the bottom of the box body. A storage battery used for supplying power to the driving assembly is arranged in the box body. An upper cover is rotationally connected to an upper opening of the box body, a fan-shaped hole is formed in the top of the upper cover in a penetrating mode, a fan-shaped cover plate used for closing the fan-shaped hole is rotationally connected into the upper cover, and a rotating assembly used for fixing the opening and closing position of the upper cover is arranged on the side wall of the upper cover; according to the utility model, by arranging the classified storage trays, different types of reagents can be stored in a classified manner; by arranging the driving assembly, automatic rotation of the classified storage disc is achieved, and a user can easily select needed reagents; by arranging the rotating assembly, the upper cover can be opened and closed, and the reagent can be integrally replaced conveniently.

The invention discloses application of a KASP molecular marker in identification of sweet melon peel color traits, and belongs to the technical field of molecular markers. The invention aims to promote gene localization and molecular-assisted breeding processes by utilizing molecular marker-assisted selective breeding on sweet melon pericarp color characters. The KASP molecular marker is a KASP marker RW2; the KASP marker RW2 is designed according to an SNP (Single Nucleotide Polymorphism) site at the position of Chr04: 526653. According to the application, the KASP molecular marker RW2 is used for performing genetic typing on the red-peel muskmelon and the white-peel muskmelon, the DNA of a target plant can be identified only through one-time PCR amplification, the whole detection process is simple and convenient, and complex steps such as enzyme digestion, electrophoresis and sequencing are not needed. Through the high-throughput genotyping system, a genotyping graph and a genotyping value can be rapidly obtained, so that rapid identification of the genotype of a target plant is realized. The KASP molecular marker RW2 provided by the invention can be more efficiently applied to the breeding work of sweet melon peel color germplasm.

The invention discloses a production process for culturing nuclear polyhedrosis virus by using an S2 or SF9 cell line. The preparation method comprises the following steps: connecting a cell-penetrating peptide C105Y with DSPE-PEG2000-Mal on the surface of a lipid nanoparticle, then preparing a lipid nanoparticle LNP-C105Y by using the DSPE-PEG2000-Mal, and entrapping nuclear polyhedrosis virus DNA to obtain DNAHcNPV LNP-C105Y; the DNAHcNPV coated LNP-C105Y is inoculated into an SF9 grassland spodoptera frugiperda cell line or an S2 fruit fly cell line to be cultured, and the nuclear polyhedrosis virus is produced. On the basis of the fall webworm nuclear polyhedrosis virus, DNAHcNPV-LNP-C105Y lipid nanoparticles are synthesized and prepared by adopting a film dispersion-extrusion method to wrap the fall webworm nuclear polyhedrosis virus, and the fall webworm nuclear polyhedrosis virus is produced by inoculating the fall webworm nuclear polyhedrosis virus to SF9 and S2 cell lines. The hyphantria cunea nuclear polyhedrosis virus production process is improved, and the defect that the hyphantria cunea nuclear polyhedrosis virus production process in the prior art cannot be separated from hyphantria cunea living body feeding is overcome.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related to a sheep body type as well as a KASP primer and application thereof, and belongs to the technical field of molecular markers. The SNP molecular marker related to the sheep body type is characterized in that a G / A polymorphic site exists at the 101st site on a DNA (Deoxyribose Nucleic Acid) sequence as shown in SEQ ID NO: 1. A KASP primer is developed on the basis of the SNP molecular marker, the KASP primer is used for detecting the genotype of the SNP molecular marker in sheep, sheep individuals with AA genotype or AA genotype and GA genotype are removed, and sheep individuals with GG genotype are reserved. According to the method, an effective means is provided for breeding of sheep body type dominant varieties, the sheep body type can be enlarged by screening the sheep carrying the G type, breeding work is simplified, meanwhile, economic benefits of sheep breeding are increased, and the method has important significance.

The invention relates to a high-throughput cis-acting element screening carrier and a screening method. Specifically, the invention provides a plasmid vector system containing bar codes, each bar code in the system is in one-to-one correspondence with a candidate cis-acting element, and the activation multiple of the candidate cis-acting element can be obtained by measuring the abundance of the bar codes; in order to eliminate the influence of a bar code on the vector on a detection result, an exogenous intron which can be cut off during transcription is inserted into a coding region in the vector and is used for distinguishing vector DNA and RNA obtained by transcription. The screening method of the biological cis-acting element has high efficiency, wide applicability and high throughput, and has outstanding application value in biological research and breeding.

The invention relates to application of tsRNA-3025a as a prognostic marker of acute myocardial infarction and a treatment target spot of myocardial ischemia reperfusion injury. A DNA (Deoxyribonucleic Acid) sequence corresponding to the tsRNA-3025a is shown as SEQ ID NO: 1: 5 '-ATCCTGCCGACTACGCCA-3'. The tsRNA-3025a can be used for treating acute myocardial infarction and myocardial ischemia reperfusion injury. In the aspect of prognosis, a detection kit is provided, and the risk of heart failure and short-term adverse events of a patient is evaluated by quantitatively detecting the expression level of the tsRNA. In the aspect of treatment, the invention provides the application of the anti-tagomir for inhibiting the function or expression of tsRNA-3025a in the preparation of the medicine for treating the myocardial ischemia reperfusion injury, and the anti-tagomir is subjected to specific chemical modification. A novel biomarker is provided for prognosis risk stratification of acute myocardial infarction, and an effective treatment strategy is provided for prevention and treatment of myocardial ischemia-reperfusion injury.

The invention belongs to the technical field of cosmetics, and particularly relates to an EGCG-DNA sodium composition with anti-oxidation and anti-aging effects and application of the EGCG-DNA sodium composition. The EGCG-DNA sodium composition is prepared from the following components in parts by weight: 0.5 to 2 parts of EGCG-DNA inclusion, and 0.5 to 2 parts of PDRN (Protein Deoxyribose Nucleic Acid) from rose. An EGCG-DNA sodium inclusion is wrapped by adopting a specific nano wrapping technology, the problems that DNA sodium is large in molecular weight and impermeable and EGCG is unstable are solved, then the rose-sourced DNA sodium is matched with one another and has a synergistic effect in a preferable dosage range, free radicals are effectively resisted, and the skin aging problem is solved.

The present disclosure, among other things, provides machine-learning technologies for identifying and localizing particular genomic elements (e.g., gene elements and / or regulatory elements) within nucleotide sequences, such as DNA and / or RNA sequences. In certain embodiments, similar to the manner in which image processing methods can be used to localize particular objects in images at pixel level resolution, referred to as “segmentation,” systems and methods of the present disclosure predict presence and locations of certain genomic elements within nucleotide sequences, thereby “segmenting” nucleotide sequences. Accordingly, genomic element segmentation technologies described herein may be used to generate annotations that identify and label portions of nucleotide sequences according to their predicted (e.g., via machine learning models described herein) function—e.g., as protein-coding genes, untranslated regions, splice sites, promotors, enhancers, etc. Among other things, these genomic annotations may be used to inform underlying biological processes driving diseases and facilitate development of new therapies.