31 results about "Pcr analysis" patented technology

The invention discloses an EV membrane protein single molecule detection method based on droplet microfluidics and application thereof. The method comprises the following steps: firstly, constructing an Ab-oligo compound which is formed by sequentially connecting an antibody, Linker, Linker connection DNA, RNA capable of being specifically cut by RNA enzyme and template DNA for starting signal amplification; then capturing the extracellular vesicles in the plasma by using the CD9 / CD63 / CD81 functionalized magnetic beads; the method comprises the following steps: adding an Ab-oligo compound and a sealing buffer solution into extracellular vesicles, incubating, removing the uncombined Ab-oligo compound, adding RNA enzyme for incubating and enzyme digestion, collecting template DNA for starting signal amplification, and performing microdroplet digital PCR analysis to realize single molecule analysis of EV membrane protein. According to the invention, the one-to-one correspondence relationship between the antibody and oligonucleotide is established through Linker for the first time, single protein information is converted into single nucleic acid chain information, and EV membrane protein single molecule detection is realized. The method is simple to operate, can qualitatively and quantitatively detect the single EV membrane protein, and is beneficial to further research on the relationship between the EV membrane protein and diseases.

The invention relates to a key gene CbLTP63 for regulating and controlling salt stress response of catalpa bungei and application of the key gene CbLTP63, and belongs to the technical field of plant genetic engineering and biology. The nucleotide sequence of the CbLTP63 gene is as shown in Seq 1, and the coded amino acid sequence is as shown in Seq 2. The invention relates to cloning of a catalpa bungei salt stress response key gene CbLTP63. Identifying that the CbLTP63-GFP subcell is positioned on a cell membrane through an agrobacterium injection method; through qRT-PCR analysis and identification, the gene responds to salt stress on the transcriptional level and is down-regulated by H2O2, ABA and SA; through transgenosis identification, the gene is a catalpa bungei salt stress response negative regulation gene, and the salt tolerance of catalpa bungei calluses after gene silencing is obviously enhanced; the invention also relates to utilization of the gene in regulating stress response of catalpa bungei. The invention discloses a catalpa bungei salt stress response key gene CbLTP63, which has important application value in the fields of catalpa bungei gene engineering and clonal forestry.

The invention discloses application of a protein dBrPRPL1 for regulating and controlling the leaf color of Chinese cabbage and a coding gene to cultivation of a colored-leaf Chinese cabbage variety with golden yellow leaf color, a specific primer is designed, and a gene is cloned from an EMS mutant of the Chinese cabbage with golden yellow leaf color by utilizing a PCR (Polymerase Chain Reaction) technology and is named as dBrPRPL1; qRT-PCR analysis shows that the expression quantity of the gene in a mutant plant in the rosette stage is obviously lower than that of a wild plant, and the expression quantity of dBrPRPL1 in other stages has no obvious difference between the mutant plant and the wild plant; the Chinese cabbage containing the dBrPRPL1 gene shows golden yellow leaf color in the seedling stage, the rosette early stage and the rosette stage, and grows slowly; the BrPRPL1 is silenced, the leaves of the plant are yellow, and the leaves of a control plant are still green. Therefore, the dBrPRPL1 is a key gene for regulating and controlling the golden leaf color of the Chinese cabbage, can be used as a gene resource for improving the leaf color of the plant, can realize precise design and breeding of plant gene editing, and has important theoretical significance and application value in the aspects of creating new germplasm and improving yield and quality.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

The utility model relates to the technical field of PCR analyzer assembly detection, in particular to a lower shell assembly detection tool of a PCR analyzer, which comprises a lower shell structure assembly, a detection circuit board and a display screen assembly, a connecting socket is arranged at the top of the detection circuit board, a connecting assembly is arranged at the top of the detection circuit board, and the display screen assembly is arranged on the lower shell structure assembly. By arranging the lower shell structure assembly, the detection circuit board and the display screen assembly, a detection structure for the lower shell element body is formed, the lower shell element body can be visually and rapidly detected, and the lower shell structure assembly, the detection circuit board and the display screen assembly are connected together and electrified. And then whether the display screen can display normally and whether touch control is normal are observed, so that the detection process of the lower shell assembly is achieved, the connection plug is fixed and connected through the arrangement of the connection assembly, the safety in the test process can be improved, the plugging reliability of the pin seat and the pin can be improved, and the situation of abnormal connection is prevented.

This invention presents a portable, battery-operated ultraviolet biofluid and fluid irradiating device, designed for deactivating pathogens in biofluids and non-terrestrial ice slurry or soil using UV LEDs. Ideal for battlefield, emergency scenarios, and low gravity or no gravity scenarios such as space travel and non-terrestrial environments. It features a microcassette for biofluid handling and PCR analysis with or without necessity for adjuvants. The device is compact, lightweight, rugged, and suitable for rapid transfusions, revolutionizing field medical care with its innovative and versatile technology which may further be adapted for non-terrestrial ice / fluid processing.

The present application belongs to the field of molecular biology detection, and particularly relates to detection of pathogenic bacteria related to respiratory tract infection, and more particularly to detection of Bordetella parapertussis, Bordetella hockingense and Bordetella bronchiseptica. The composition for combined detection provided by the present application mainly utilizes a multiplex fluorescence PCR analysis method, and different pathogenic bacteria are detected by detecting target points on different pathogenic bacteria, so that detection and differentiation of Bordetella parapertussis, Bordetella hockingense and Bordetella bronchiseptica are simultaneously achieved in a single tube reaction system. The composition of the present application has higher sensitivity, reaches 500 copies / mL, has good specificity, and is more accurate in detection.

The present invention relates to a recombinant vector for genome editing in rice. The vector comprises of GluB1 promoter of rice along with Cas 12 a gene and a guide RNA. Editing the targeted gene within localized tissue effectively mitigates the pleiotropic effect observed in non- targeted tissues, thus reducing the potential for unintended consequences. Tissue-specific genome editing (TSGE) plays a pivotal role in alleviating the metabolic burden on plants by restricting modifications to specific areas. The designed CRISPR-ESGE binary construct was introduced into Agrobacterium, which was then used to infect rice callus derived from seeds. The regenerated transformed plants were confirmed to contain the Cas12a gene, and the endosperm-specific expression of Cas12a was validated through RT-PCR analysis. Genome editing (GE) utilizing Cas12a offers distinct advantages over Cas9, which is characterized by heightened target specificity and decreased propensity for off-target effect, thereby potentially enhancing the grain starch content.

Various embodiments for a chip for use in a real-time qPCR system are disclosed. The chip can include at least one port for receiving a sample into the chip; at least one channel in fluidic communication with the at least port; a plurality of magnetically active beads disposed within the at least one channel that capture DNA / RNA from the sample as the sample passes through the at least one channel; and an optical inspection region in fluidic communication with the at least one channel for performing an optical analysis of the sample containing the eluted DNA / RNA previously captured on the magnetic beads.

The invention relates to the cartridge elevating mechanism that ensures the necessary movement of the cartridge and maintains the process quality by opening and closing the cover in devices where isolation, PCR and similar analyzes are performed. The invention particularly relates to a cartridge elevating mechanism configuration developed to eliminate the negativities related to the cartridge encountered in the use of devices that perform the isolation process to purify target molecules such as DNA, RNA or protein from biological samples, the setup process for the preparation of PCR reactions, and the real-time PCR analysis for the quantitative determination of target DNA.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

The invention relates to the technical field of gene engineering application, and particularly discloses a key gene LiRabA4a for regulating and controlling the leaf color of crape myrtle and application thereof. The nucleotide sequence of the gene LiRabA4a is as shown in Seq 1, and the coded amino acid sequence is as shown in Seq 2. The invention relates to cloning of a key gene LiRabA4a for regulating and controlling the leaf color of lagerstroemia indica. Through qRT-PCR analysis, it is found that the LiRabA4a gene is remarkably predominantly expressed in crape myrtle leaves, and the transcriptional level of the LiRabA4a gene is related to the leaf color yellowing degree; the LiRabA4a gene is overexpressed through an agrobacterium tumefaciens-mediated genetic transformation method, so that the chlorophyll content of the crape myrtle can be increased, and the leaf color is greener; liRabA4a gene expression is inhibited, so that the chlorophyll content is reduced, and the leaf color is yellow; the invention further relates to heterologous expression of the LiRabA4a gene in catalpa bungei for remarkably promoting growth and development of catalpa bungei. The LiRabA4a gene provided by the invention can be used for directional genetic improvement of the leaf color of the lagerstroemia indica, can be used for cultivating lagerstroemia indica varieties with different leaf colors, and has an important application value in the aspects of gene engineering and molecular design breeding of the lagerstroemia indica.

The utility model relates to a lower shell assembly of a real-time fluorescence PCR analyzer, which comprises a lower shell and a light bar, the lower shell is of a Z-shaped structure, the front side wall of the lower shell is provided with a light strip groove, the light strip groove is horizontally arranged, the length size of the light strip groove is 2 / 3-4 / 5 of the length size of the front side wall of the lower shell, two ends of the inner side of the light strip groove are provided with supporting columns, and the supporting columns are connected with the light strip. A light uniformizing piece is arranged on the outer side of the light emitting face of the lamp strip, the two sides of the light uniformizing piece are inserted into the supporting columns, and the outer surface of the light uniformizing piece protrudes out of the outer side of the lamp strip groove. Compared with traditional PCR equipment, the real-time fluorescent PCR analyzer can obviously remind medical staff that the PCR analyzer is started and runs from the visual aspect, and due to the fact that the light bar is long and the brightness range is large, a certain lighting effect can be provided for a user.

The utility model discloses a PCR (Polymerase Chain Reaction) analyzer base structure, which relates to the technical field of bases and comprises a PCR analyzer body, and a base is arranged at the bottom end of the PCR analyzer body; the base comprises a circular shell, a cooling fan is installed in the shell, and four supporting rods are annularly arranged on the surface of the shell at equal intervals. According to the PCR analyzer disclosed by the utility model, the heat dissipation fan is arranged on the base, so that the bottom end of the PCR analyzer can be cooled, the problem that the bottom end of the PCR analyzer is close to a working table surface and is difficult to cool in a short time under long-time work is avoided, and meanwhile, a movable block can drive a supporting strip and a base plate to move by rotating a screw rod; the height of the base is adjusted conveniently, so that the overall height of the PCR analyzer can be adjusted according to the heights of different workbenches, the convenience and comfort of operation are improved, and the bottom end of the cooling fan can be far away from the workbenches, so that better heat dissipation is facilitated.

The invention is directed to compositions and methods for collecting, transporting, and storing, without refrigeration, biological materials, which may comprise samples of biological, clinical, forensic, and / or environmental origin. These compositions preserve the viability of the collected organisms and / or the RNA / DNA and proteins in the sample composition mixture and permit the long-term storage of samples. Compositions are compatible with subsequent manipulation of the sample, including propagation and culture of the collected microorganisms, or isolation, purification, detection, and characterization of proteins, nucleic acids, and other macromolecules. When the compositions containing microorganisms and any polynucleotides therein are further processed, such as by nucleic acid testing, there is an increased ability to detect, isolate, purify and / or characterize select microbes and their components, such as nucleic acids, when compared to conventional microbial transport media that contain interfering substances and RNA / DNA extraction is not required prior to PCR analysis. In particular, the compositions disclosed allow for the collection, transport and storage of biological samples for extended periods at ambient temperature, while maintaining the integrity of the macromolecules of the sample for subsequent extraction, identification, and quantitation.

The present invention relates to a novel simultaneous multiple all-in-one LOP molecular diagnostic platform structure and a simultaneous multiple molecular diagnostic kit for diagnosing shrimp white spot virus, acute hepatopancreatic necrosis, and microspore infection using same. According to the present invention, shrimp white spot virus, acute hepatopancreatic necrosis, and microspore infection that necessitated PCR analysis can now be easily and simultaneously detected on-site via simultaneous multiple detection.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

An automated polymerase chain reaction (PCR) analyzer (110) for analyzing at least one sample (112) and a method of automated PCR analysis is disclosed. The automated PCR analyzer (110) comprises: - at least one controller (114); - at least one thermocycling apparatus (116) configured for subjecting the sample (112) to at least one temperature modulation controlled by the controller (114); and - at least one photometer (118) configured for detecting at least one optically detectable property of the sample (112), the photometer (118) comprising at least one excitation light source (120) configured for illuminating the sample (112) with excitation light (122), the photometer (118) further comprising at least one photodetector unit (124) configured for detecting light (123) emitted from the sample (112), the photometer (118) further comprising a movable filter cell (126) controllable by the controller (114), the movable filter cell (126) comprising a plurality of filter cell units (128), each filter cell unit (128) comprising at least one optical excitation filter element (130), at least one dichroic filter (132), at least one optical detection filter element (134) and at least one the aperture stop (174), wherein the photometer (118) comprises a working position for the filter cell units (128), wherein the movable filter cell (126) is configured for selectively positioning a selected filter cell unit (128) selected from the plurality of filter cell units (128) in the working position, wherein, in the working position, the selected filter cell unit (128) is positioned such that the excitation light (122) interacts with the optical excitation filter element (130), the dichroic filter (132) and the aperture stop (174) of the selected filter cell unit (128) and that the light (123) emitted from the sample (112) interacts with the dichroic filter (132), the aperture stop (174) and the optical detection filter element (134) of the selected filter cell unit (128).

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

The invention relates to a free fetal DNA enrichment and quantitative analysis method based on a nano magnetic bead. The method comprises the following steps: preparing the nano magnetic bead of which the surface is modified with a polyethylene glycol (PEG) chain brush, regulating and controlling the density and spatial arrangement of the PEG chain, forming a structure screening layer adaptive to free DNA with the length of 140-160bp on the surface of the magnetic bead, and capturing short fragment DNA to finish selective enrichment; adding the functionalized nano magnetic beads into a pregnant woman plasma reaction system, and applying a directional electric field to guide negatively charged DNA to migrate to the surfaces of the magnetic beads to form a local focusing area of free fetal DNA; after adsorption and enrichment are completed, the pH value or temperature of a reaction system is adjusted, the structural tension gradient of a PEG chain brush area is excited, target fetal DNA with medium binding force is released from the surfaces of the magnetic beads, and specific desorption is completed; and introducing the released DNA solution into a microcavity structure provided with a micro-resistance flow channel, and only collecting DNA components which are relatively high in diffusion speed and high in leading edge centrality to carry out subsequent fluorescent quantitative PCR or digital PCR analysis so as to finish high-purity quantitative detection of the target fetal DNA.

The present application relates to a kind of regulation Catalpa bungei salt stress response key gene CbLTP63 and its application, belong to plant genetic engineering and biotechnology field.CbLTP63 gene nucleotide sequence is as described in Seq 1, coding amino acid sequence is as described in Seq 2.The present application relates to the cloning of Catalpa bungei salt stress response key gene CbLTP63;CbLTP63-GFP subcellular localization is positioned on cell membrane by identifying agrobacterium injection method;By qRT-PCR analysis, it is identified that the gene responds to salt stress at transcription level, is down-regulated by H2O2, ABA, SA;By transgenic identification, the gene is Catalpa bungei salt stress response negative regulation gene, and the salt tolerance of Catalpa bungei callus after gene silencing is significantly enhanced;Also related to the use of the gene regulation Catalpa bungei stress response.The present application discloses a kind of Catalpa bungei salt stress response key gene CbLTP63, and has important application value in the field of Catalpa bungei genetic engineering and clone forestry.

A device (1) for PCR analysis comprises a loading unit (2) having a first movement mechanism to move a sample holder receiving one or more samples from one zone to another between an entry zone and a loading zone, the device (1) comprising a second movement mechanism (5) to move a sample holder receiving one or more samples from one zone to another between an entry zone and a loading zone, the second movement mechanism is configured to move at least one sample between two zones selected from a list comprising the loading zone, a thermal and / or pressure zone of the thermal and / or pressure unit (3) and a scanning zone of the scanning unit (4). A method for controlling such an apparatus (1), and a corresponding computer program and computer-readable storage medium.

The present invention relates to the technical field of digital PCR analysis, and in particular to a digital PCR analysis device and an analysis method thereof. The present invention comprises a droplet generation module, a PCR amplification module, a feature extraction module, a feature determination module, a ventilation module, and a fluorescence detection module. The droplet generation module is used to obtain a plurality of droplet units. The PCR amplification module is used to denature, anneal, and extend each droplet unit. The feature extraction module is used to obtain a bubble floating tendency characterization value. The feature determination module is used to screen droplet feature areas, select an annealing adjustment method, and determine whether to continue PCR amplification. The ventilation module is used to adjust the wind speed and temperature of the inlet air. The adjustment and verification unit is used to adjust and verify the droplet units. The output unit outputs the analysis results. Thus, during the denaturation process of PCR amplification, annealing can be adaptively adjusted according to the actual state of the droplet unit, thereby improving the accuracy of the digital PCR analysis results.

The application discloses a large white pig genome prediction method based on improved genome feature principal component regression, which is defined as GF_PCR. The method divides the traditional PCR method into two parts, performs simple regression on the genome feature part, and performs PCR analysis on the remaining SNPs. When the genome feature contains more causal mutations, the model can obtain higher genome prediction accuracy. At the same time, the genome prediction accuracy of the GF_PCR model for the 100Kg body weight day age trait is higher than that of the PCR model, and the reason is that the proportion of the causal mutation in the preselected genome feature is larger, and the GF_PCR further improves the genome prediction accuracy.

This invention provides a method for determining the minimum number of droplets required for droplet-based digital PCR analysis, relating to the field of PCR detection technology. The method includes: obtaining target parameters, including gene copy number, theoretical droplet number, target confidence level, and target error; and determining the minimum number of droplets corresponding to the target parameters based on a pre-established theoretical model relating droplet number to confidence level and error. The theoretical model relating droplet number to confidence level and error is based on the n-fold Bernoulli distribution theory and Chebyshev's inequality. This method theoretically determines the minimum number of droplets required for droplet-based digital PCR analysis, alleviating the problem of currently determining the minimum droplet number only empirically. It is easy to understand and implement, thereby improving the consistency and reliability of the analytical results.

The invention discloses a molecular breeding technology for realizing regulation and control of the flower diameter of chrysanthemum through single-gene silencing and application of the molecular breeding technology. On the basis of a CmRAD6 gene identified in chrysanthemum'mirama ', a specific artificial amiRNA interference element is designed, a plant expression vector is constructed, a chrysanthemum explant is transformed by adopting an agrobacterium tumefaciens-mediated leaf disc transformation method, and a transgenic plant is obtained through kanamycin resistance screening. Molecular verification shows that exogenous genes are successfully integrated, and qRT-PCR (quantitative reverse transcription-polymerase chain reaction) analysis shows that the expression quantity of CmRAD6 in transgenic plants is obviously reduced compared with that of wild plants. Phenotypic observation shows that the longitudinal diameter of the ligulate flower of the transgenic plant is shortened, the diameter of the corolla is reduced, and the character is stably inherited for more than two years. The new function of the CmRAD6 gene for regulating and controlling the development of the floral organs of the compositae plants is disclosed for the first time, the bottleneck of the traditional breeding technology is broken through through single-gene accurate regulation and control, the energy consumption of facility cultivation can be reduced, and an innovative technical scheme is provided for molecular design and breeding of flowers.

This utility model relates to the field of medical device technology, specifically disclosing a cap-switching device and a PCR analysis device, including a carrier plate, a clamping mechanism, and a vertical moving mechanism. The clamping mechanism includes a support bracket, a first driving component, a pressing component, and a snap-fit ​​component. The support bracket is movably mounted on the carrier plate along the Z-direction, and the first driving component and the snap-fit ​​component are both mounted on the support bracket. The driving end of the first driving component is connected to the pressing component. The vertical moving mechanism is mounted on the carrier plate, and its driving end is connected to the support bracket. The cap-switching device of this utility model enables the opening and closing of reaction consumables, avoiding the difficulty of opening and closing caps caused by tilting of the tube caps. It is not only highly efficient but also prevents deformation of the tube body or the tube caps, thereby reducing the probability of errors and improving the accuracy and reliability of the tube caps being mounted on the tube body. It also reduces labor costs and the risk of contamination.