53 results about "Pcr analysis" patented technology

The invention belongs to the field of molecular biological detection, and provides a joint detection composition which mainly utilizes a multiple fluorescent PCR analysis method to detect related pathogens, so as to simultaneously realize detection of coronavirus, bordetella pertussis, group A streptococcus, parainfluenza virus, streptococcus pneumoniae, haemophilus influenzae and group B streptococcus in a single-tube reaction system. Or detecting and distinguishing at least four of chlamydia pneumoniae. Meanwhile, the detection sensitivity of the composition is higher and reaches 500 copies / mL, and the detection is more accurate.

The invention discloses an EV membrane protein single molecule detection method based on droplet microfluidics and application thereof. The method comprises the following steps: firstly, constructing an Ab-oligo compound which is formed by sequentially connecting an antibody, Linker, Linker connection DNA, RNA capable of being specifically cut by RNA enzyme and template DNA for starting signal amplification; then capturing the extracellular vesicles in the plasma by using the CD9 / CD63 / CD81 functionalized magnetic beads; the method comprises the following steps: adding an Ab-oligo compound and a sealing buffer solution into extracellular vesicles, incubating, removing the uncombined Ab-oligo compound, adding RNA enzyme for incubating and enzyme digestion, collecting template DNA for starting signal amplification, and performing microdroplet digital PCR analysis to realize single molecule analysis of EV membrane protein. According to the invention, the one-to-one correspondence relationship between the antibody and oligonucleotide is established through Linker for the first time, single protein information is converted into single nucleic acid chain information, and EV membrane protein single molecule detection is realized. The method is simple to operate, can qualitatively and quantitatively detect the single EV membrane protein, and is beneficial to further research on the relationship between the EV membrane protein and diseases.

The invention relates to a key gene CbLTP63 for regulating and controlling salt stress response of catalpa bungei and application of the key gene CbLTP63, and belongs to the technical field of plant genetic engineering and biology. The nucleotide sequence of the CbLTP63 gene is as shown in Seq 1, and the coded amino acid sequence is as shown in Seq 2. The invention relates to cloning of a catalpa bungei salt stress response key gene CbLTP63. Identifying that the CbLTP63-GFP subcell is positioned on a cell membrane through an agrobacterium injection method; through qRT-PCR analysis and identification, the gene responds to salt stress on the transcriptional level and is down-regulated by H2O2, ABA and SA; through transgenosis identification, the gene is a catalpa bungei salt stress response negative regulation gene, and the salt tolerance of catalpa bungei calluses after gene silencing is obviously enhanced; the invention also relates to utilization of the gene in regulating stress response of catalpa bungei. The invention discloses a catalpa bungei salt stress response key gene CbLTP63, which has important application value in the fields of catalpa bungei gene engineering and clonal forestry.

Provided herein is a system, a device that includes modules, a cartridge that includes components such as a microfluidic channels, and methods that relate to detecting nucleic acids and other constituents of biological samples. The present disclosure also relates to cartridges, devices, and methods for performing sample analysis, e.g., nucleic acid analysis such as PCR analysis of materials within the cartridges in a rapid manner.

The invention belongs to the field of molecular biology detection, particularly relates to detection of blood flow infection related pathogens, and more particularly relates to detection and distinguishing of at least four pathogens in burkholderia cepacia, streptococcus mirabilis, staphylococcus capitis, staphylococcus coriolis, proteus mirabilis and staphylococcus haemolyticus. According to the combined detection composition provided by the invention, different pathogens are detected by mainly utilizing a multiple fluorescent PCR analysis method and detecting target spots on the different pathogens; therefore, at least four pathogens in burkholderia cepacia, streptococcus mitis, staphylococcus capitis, staphylococcus coriolis, proteus mirabilis and staphylococcus haemolyticus are detected and distinguished in a single-tube reaction system at the same time, and the detection efficiency is improved. The composition disclosed by the invention is high in detection sensitivity, and the detection sensitivity reaches 200 copies / mL; the reagent / kit provided by the invention adopts a UNG enzyme and dUTP anti-pollution system, so that the accuracy of the detection effect can be guaranteed.

The invention discloses application of a protein dBrPRPL1 for regulating and controlling the leaf color of Chinese cabbage and a coding gene to cultivation of a colored-leaf Chinese cabbage variety with golden yellow leaf color, a specific primer is designed, and a gene is cloned from an EMS mutant of the Chinese cabbage with golden yellow leaf color by utilizing a PCR (Polymerase Chain Reaction) technology and is named as dBrPRPL1; qRT-PCR analysis shows that the expression quantity of the gene in a mutant plant in the rosette stage is obviously lower than that of a wild plant, and the expression quantity of dBrPRPL1 in other stages has no obvious difference between the mutant plant and the wild plant; the Chinese cabbage containing the dBrPRPL1 gene shows golden yellow leaf color in the seedling stage, the rosette early stage and the rosette stage, and grows slowly; the BrPRPL1 is silenced, the leaves of the plant are yellow, and the leaves of a control plant are still green. Therefore, the dBrPRPL1 is a key gene for regulating and controlling the golden leaf color of the Chinese cabbage, can be used as a gene resource for improving the leaf color of the plant, can realize precise design and breeding of plant gene editing, and has important theoretical significance and application value in the aspects of creating new germplasm and improving yield and quality.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

The present invention relates to a substrate for nucleic acid amplification, and a method for manufacturing same, the substrate for rapid and accurate PCR analysis comprising: a transparent substrate; a metal layer formed on the transparent substrate; an N-heterocyclic carbene compound having one end annealed to the surface of the metal layer; and a primer immobilized on the other end of the N-heterocyclic carbene compound.

The present invention relates to the structure of an analysis plate applied to a high-speed polymerase chain reaction (PCR), and to a PCR analysis plate used for implementing an analysis of a real-time PCR, a real-time nested PCR and a post-PCR lateral flow hybridization reaction. The present invention is provided with: a check valve for enabling the maintaining of positive pressure when an elastic film expands into a convex form by having a solution pushed therein by the positive pressure; a lateral flow analysis module for analyzing a post-PCR follow-up PCR or lateral flow; and a shut-off valve enabling the controlling of the movement of the solution after each reaction ends.

The utility model relates to the technical field of PCR analyzer assembly detection, in particular to a lower shell assembly detection tool of a PCR analyzer, which comprises a lower shell structure assembly, a detection circuit board and a display screen assembly, a connecting socket is arranged at the top of the detection circuit board, a connecting assembly is arranged at the top of the detection circuit board, and the display screen assembly is arranged on the lower shell structure assembly. By arranging the lower shell structure assembly, the detection circuit board and the display screen assembly, a detection structure for the lower shell element body is formed, the lower shell element body can be visually and rapidly detected, and the lower shell structure assembly, the detection circuit board and the display screen assembly are connected together and electrified. And then whether the display screen can display normally and whether touch control is normal are observed, so that the detection process of the lower shell assembly is achieved, the connection plug is fixed and connected through the arrangement of the connection assembly, the safety in the test process can be improved, the plugging reliability of the pin seat and the pin can be improved, and the situation of abnormal connection is prevented.

This invention presents a portable, battery-operated ultraviolet biofluid and fluid irradiating device, designed for deactivating pathogens in biofluids and non-terrestrial ice slurry or soil using UV LEDs. Ideal for battlefield, emergency scenarios, and low gravity or no gravity scenarios such as space travel and non-terrestrial environments. It features a microcassette for biofluid handling and PCR analysis with or without necessity for adjuvants. The device is compact, lightweight, rugged, and suitable for rapid transfusions, revolutionizing field medical care with its innovative and versatile technology which may further be adapted for non-terrestrial ice / fluid processing.

The present application belongs to the field of molecular biology detection, and particularly relates to detection of pathogenic bacteria related to respiratory tract infection, and more particularly to detection of Bordetella parapertussis, Bordetella hockingense and Bordetella bronchiseptica. The composition for combined detection provided by the present application mainly utilizes a multiplex fluorescence PCR analysis method, and different pathogenic bacteria are detected by detecting target points on different pathogenic bacteria, so that detection and differentiation of Bordetella parapertussis, Bordetella hockingense and Bordetella bronchiseptica are simultaneously achieved in a single tube reaction system. The composition of the present application has higher sensitivity, reaches 500 copies / mL, has good specificity, and is more accurate in detection.

The present invention relates to a recombinant vector for genome editing in rice. The vector comprises of GluB1 promoter of rice along with Cas 12 a gene and a guide RNA. Editing the targeted gene within localized tissue effectively mitigates the pleiotropic effect observed in non- targeted tissues, thus reducing the potential for unintended consequences. Tissue-specific genome editing (TSGE) plays a pivotal role in alleviating the metabolic burden on plants by restricting modifications to specific areas. The designed CRISPR-ESGE binary construct was introduced into Agrobacterium, which was then used to infect rice callus derived from seeds. The regenerated transformed plants were confirmed to contain the Cas12a gene, and the endosperm-specific expression of Cas12a was validated through RT-PCR analysis. Genome editing (GE) utilizing Cas12a offers distinct advantages over Cas9, which is characterized by heightened target specificity and decreased propensity for off-target effect, thereby potentially enhancing the grain starch content.

Various embodiments for a chip for use in a real-time qPCR system are disclosed. The chip can include at least one port for receiving a sample into the chip; at least one channel in fluidic communication with the at least port; a plurality of magnetically active beads disposed within the at least one channel that capture DNA / RNA from the sample as the sample passes through the at least one channel; and an optical inspection region in fluidic communication with the at least one channel for performing an optical analysis of the sample containing the eluted DNA / RNA previously captured on the magnetic beads.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

The invention belongs to the field of molecular biology detection, particularly relates to detection of wound infection related pathogens, and more particularly relates to detection of klebsiella pneumoniae, staphylococcus aureus and providencia stuartii. According to the combined detection composition provided by the invention, different pathogens are detected by mainly utilizing a multiple fluorescent PCR analysis method and detecting target spots on the different pathogens, so that detection and distinguishing of klebsiella pneumoniae, staphylococcus aureus and providencia stuartii are simultaneously realized in a single-tube reaction system; therefore, a treatment strategy can be provided in a targeted manner. The composition disclosed by the invention is higher in detection sensitivity (up to 400 copies / mL), good in specificity and more accurate in detection.

The invention relates to the cartridge elevating mechanism that ensures the necessary movement of the cartridge and maintains the process quality by opening and closing the cover in devices where isolation, PCR and similar analyzes are performed. The invention particularly relates to a cartridge elevating mechanism configuration developed to eliminate the negativities related to the cartridge encountered in the use of devices that perform the isolation process to purify target molecules such as DNA, RNA or protein from biological samples, the setup process for the preparation of PCR reactions, and the real-time PCR analysis for the quantitative determination of target DNA.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

The invention relates to the technical field of gene engineering application, and particularly discloses a key gene LiRabA4a for regulating and controlling the leaf color of crape myrtle and application thereof. The nucleotide sequence of the gene LiRabA4a is as shown in Seq 1, and the coded amino acid sequence is as shown in Seq 2. The invention relates to cloning of a key gene LiRabA4a for regulating and controlling the leaf color of lagerstroemia indica. Through qRT-PCR analysis, it is found that the LiRabA4a gene is remarkably predominantly expressed in crape myrtle leaves, and the transcriptional level of the LiRabA4a gene is related to the leaf color yellowing degree; the LiRabA4a gene is overexpressed through an agrobacterium tumefaciens-mediated genetic transformation method, so that the chlorophyll content of the crape myrtle can be increased, and the leaf color is greener; liRabA4a gene expression is inhibited, so that the chlorophyll content is reduced, and the leaf color is yellow; the invention further relates to heterologous expression of the LiRabA4a gene in catalpa bungei for remarkably promoting growth and development of catalpa bungei. The LiRabA4a gene provided by the invention can be used for directional genetic improvement of the leaf color of the lagerstroemia indica, can be used for cultivating lagerstroemia indica varieties with different leaf colors, and has an important application value in the aspects of gene engineering and molecular design breeding of the lagerstroemia indica.

The utility model relates to a lower shell assembly of a real-time fluorescence PCR analyzer, which comprises a lower shell and a light bar, the lower shell is of a Z-shaped structure, the front side wall of the lower shell is provided with a light strip groove, the light strip groove is horizontally arranged, the length size of the light strip groove is 2 / 3-4 / 5 of the length size of the front side wall of the lower shell, two ends of the inner side of the light strip groove are provided with supporting columns, and the supporting columns are connected with the light strip. A light uniformizing piece is arranged on the outer side of the light emitting face of the lamp strip, the two sides of the light uniformizing piece are inserted into the supporting columns, and the outer surface of the light uniformizing piece protrudes out of the outer side of the lamp strip groove. Compared with traditional PCR equipment, the real-time fluorescent PCR analyzer can obviously remind medical staff that the PCR analyzer is started and runs from the visual aspect, and due to the fact that the light bar is long and the brightness range is large, a certain lighting effect can be provided for a user.

The utility model discloses a PCR (Polymerase Chain Reaction) analyzer base structure, which relates to the technical field of bases and comprises a PCR analyzer body, and a base is arranged at the bottom end of the PCR analyzer body; the base comprises a circular shell, a cooling fan is installed in the shell, and four supporting rods are annularly arranged on the surface of the shell at equal intervals. According to the PCR analyzer disclosed by the utility model, the heat dissipation fan is arranged on the base, so that the bottom end of the PCR analyzer can be cooled, the problem that the bottom end of the PCR analyzer is close to a working table surface and is difficult to cool in a short time under long-time work is avoided, and meanwhile, a movable block can drive a supporting strip and a base plate to move by rotating a screw rod; the height of the base is adjusted conveniently, so that the overall height of the PCR analyzer can be adjusted according to the heights of different workbenches, the convenience and comfort of operation are improved, and the bottom end of the cooling fan can be far away from the workbenches, so that better heat dissipation is facilitated.

The invention provides a reference gene screening method for fluorogenic quantitative PCR (Polymerase Chain Reaction) analysis of peach-necked longhorn beetles and application of the reference gene screening method. The specific scheme is as follows: RPL13 and RPL10 are selected as reference gene combinations in different development stages, and alpha-tubulin and SOD are selected as reference gene combinations in different tissues. The nucleotide sequences are respectively ID 1, ID 2, ID 3 and ID 4. In addition, the invention also provides different primer sequences used when the stably expressed reference gene is used for qRT-PCR (quantitative reverse transcription-polymerase chain reaction) analysis. The method comprises the following steps: selecting 10 candidate genes, applying a qRT-PCR (quantitative reverse transcription-polymerase chain reaction) technology, combining GeNorm, BestKeeper, a delta Ct method, NormFinder and RefFinder software for analysis, and screening out a reference gene combination which is stably expressed under different experimental conditions. The screening system provides important technical support for the gene function research of the peach-necked longhorn beetles, and provides a scientific basis for the research of the peach-necked longhorn beetles by utilizing molecular biology.

The invention is directed to compositions and methods for collecting, transporting, and storing, without refrigeration, biological materials, which may comprise samples of biological, clinical, forensic, and / or environmental origin. These compositions preserve the viability of the collected organisms and / or the RNA / DNA and proteins in the sample composition mixture and permit the long-term storage of samples. Compositions are compatible with subsequent manipulation of the sample, including propagation and culture of the collected microorganisms, or isolation, purification, detection, and characterization of proteins, nucleic acids, and other macromolecules. When the compositions containing microorganisms and any polynucleotides therein are further processed, such as by nucleic acid testing, there is an increased ability to detect, isolate, purify and / or characterize select microbes and their components, such as nucleic acids, when compared to conventional microbial transport media that contain interfering substances and RNA / DNA extraction is not required prior to PCR analysis. In particular, the compositions disclosed allow for the collection, transport and storage of biological samples for extended periods at ambient temperature, while maintaining the integrity of the macromolecules of the sample for subsequent extraction, identification, and quantitation.

The invention belongs to the field of molecular biological detection, and provides a combined detection composition which is mainly used for detecting diarrhea-related pathogen viruses by using a multiple fluorescent PCR (Polymerase Chain Reaction) analysis method. Therefore, at least four of human cystoprotozoa, cyclosporidium, binuclear ameeba fragilis, cryptosporidium, giardia and entamoeba histolytica can be detected and distinguished in a single-tube reaction system at the same time. Meanwhile, the detection sensitivity of the composition is higher and reaches 200 copies / mL, and the detection is more accurate.

The present invention relates to a novel simultaneous multiple all-in-one LOP molecular diagnostic platform structure and a simultaneous multiple molecular diagnostic kit for diagnosing shrimp white spot virus, acute hepatopancreatic necrosis, and microspore infection using same. According to the present invention, shrimp white spot virus, acute hepatopancreatic necrosis, and microspore infection that necessitated PCR analysis can now be easily and simultaneously detected on-site via simultaneous multiple detection.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

Provided herein is a system, a device that includes modules, a cartridge that includes components such as a microfluidic channels, and methods that relate to detecting nucleic acids and other constituents of biological samples. The present disclosure also relates to cartridges, devices, and methods for performing sample analysis, e.g., nucleic acid analysis such as PCR analysis of materials within the cartridges in a rapid manner.

The utility model discloses a calibration device. The calibration device comprises a calibration main body, a position calibration mechanism and a temperature calibration mechanism, the position calibration mechanism comprises a position calibration part, a first position detector and a second position detector, and in the process that the PCR analysis module pushes the position calibration part to move relative to the calibration main body, the first position detector is matched with the position calibration part and detects Y-axis coordinates of the PCR analysis module at an amplification position; in the process that the pipetting extraction module moves relative to the calibration main body, the second position detector can detect Y-axis and Z-axis coordinates of the pipetting extraction module at an extraction position; the temperature calibration mechanism comprises a first temperature detection part and a second temperature detection part, the first temperature detection part is used for detecting the temperature of the PCR analysis module, and the second temperature detection part is used for detecting the heating temperature of the heating module. Position calibration and temperature calibration are integrated on the calibration main body, the working position and temperature of the POCT all-in-one machine are automatically adjusted, measured and calibrated, and the calibration efficiency of the POCT all-in-one machine is improved.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.