21 results about "Pcr analysis" patented technology

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

The utility model relates to the technical field of PCR analyzer assembly detection, in particular to a lower shell assembly detection tool of a PCR analyzer, which comprises a lower shell structure assembly, a detection circuit board and a display screen assembly, a connecting socket is arranged at the top of the detection circuit board, a connecting assembly is arranged at the top of the detection circuit board, and the display screen assembly is arranged on the lower shell structure assembly. By arranging the lower shell structure assembly, the detection circuit board and the display screen assembly, a detection structure for the lower shell element body is formed, the lower shell element body can be visually and rapidly detected, and the lower shell structure assembly, the detection circuit board and the display screen assembly are connected together and electrified. And then whether the display screen can display normally and whether touch control is normal are observed, so that the detection process of the lower shell assembly is achieved, the connection plug is fixed and connected through the arrangement of the connection assembly, the safety in the test process can be improved, the plugging reliability of the pin seat and the pin can be improved, and the situation of abnormal connection is prevented.

The present application belongs to the field of molecular biology detection, and particularly relates to detection of pathogenic bacteria related to respiratory tract infection, and more particularly to detection of Bordetella parapertussis, Bordetella hockingense and Bordetella bronchiseptica. The composition for combined detection provided by the present application mainly utilizes a multiplex fluorescence PCR analysis method, and different pathogenic bacteria are detected by detecting target points on different pathogenic bacteria, so that detection and differentiation of Bordetella parapertussis, Bordetella hockingense and Bordetella bronchiseptica are simultaneously achieved in a single tube reaction system. The composition of the present application has higher sensitivity, reaches 500 copies / mL, has good specificity, and is more accurate in detection.

The present invention relates to a recombinant vector for genome editing in rice. The vector comprises of GluB1 promoter of rice along with Cas 12 a gene and a guide RNA. Editing the targeted gene within localized tissue effectively mitigates the pleiotropic effect observed in non- targeted tissues, thus reducing the potential for unintended consequences. Tissue-specific genome editing (TSGE) plays a pivotal role in alleviating the metabolic burden on plants by restricting modifications to specific areas. The designed CRISPR-ESGE binary construct was introduced into Agrobacterium, which was then used to infect rice callus derived from seeds. The regenerated transformed plants were confirmed to contain the Cas12a gene, and the endosperm-specific expression of Cas12a was validated through RT-PCR analysis. Genome editing (GE) utilizing Cas12a offers distinct advantages over Cas9, which is characterized by heightened target specificity and decreased propensity for off-target effect, thereby potentially enhancing the grain starch content.

Various embodiments for a chip for use in a real-time qPCR system are disclosed. The chip can include at least one port for receiving a sample into the chip; at least one channel in fluidic communication with the at least port; a plurality of magnetically active beads disposed within the at least one channel that capture DNA / RNA from the sample as the sample passes through the at least one channel; and an optical inspection region in fluidic communication with the at least one channel for performing an optical analysis of the sample containing the eluted DNA / RNA previously captured on the magnetic beads.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

The invention relates to the technical field of gene engineering application, and particularly discloses a key gene LiRabA4a for regulating and controlling the leaf color of crape myrtle and application thereof. The nucleotide sequence of the gene LiRabA4a is as shown in Seq 1, and the coded amino acid sequence is as shown in Seq 2. The invention relates to cloning of a key gene LiRabA4a for regulating and controlling the leaf color of lagerstroemia indica. Through qRT-PCR analysis, it is found that the LiRabA4a gene is remarkably predominantly expressed in crape myrtle leaves, and the transcriptional level of the LiRabA4a gene is related to the leaf color yellowing degree; the LiRabA4a gene is overexpressed through an agrobacterium tumefaciens-mediated genetic transformation method, so that the chlorophyll content of the crape myrtle can be increased, and the leaf color is greener; liRabA4a gene expression is inhibited, so that the chlorophyll content is reduced, and the leaf color is yellow; the invention further relates to heterologous expression of the LiRabA4a gene in catalpa bungei for remarkably promoting growth and development of catalpa bungei. The LiRabA4a gene provided by the invention can be used for directional genetic improvement of the leaf color of the lagerstroemia indica, can be used for cultivating lagerstroemia indica varieties with different leaf colors, and has an important application value in the aspects of gene engineering and molecular design breeding of the lagerstroemia indica.

The utility model relates to a lower shell assembly of a real-time fluorescence PCR analyzer, which comprises a lower shell and a light bar, the lower shell is of a Z-shaped structure, the front side wall of the lower shell is provided with a light strip groove, the light strip groove is horizontally arranged, the length size of the light strip groove is 2 / 3-4 / 5 of the length size of the front side wall of the lower shell, two ends of the inner side of the light strip groove are provided with supporting columns, and the supporting columns are connected with the light strip. A light uniformizing piece is arranged on the outer side of the light emitting face of the lamp strip, the two sides of the light uniformizing piece are inserted into the supporting columns, and the outer surface of the light uniformizing piece protrudes out of the outer side of the lamp strip groove. Compared with traditional PCR equipment, the real-time fluorescent PCR analyzer can obviously remind medical staff that the PCR analyzer is started and runs from the visual aspect, and due to the fact that the light bar is long and the brightness range is large, a certain lighting effect can be provided for a user.

The utility model discloses a PCR (Polymerase Chain Reaction) analyzer base structure, which relates to the technical field of bases and comprises a PCR analyzer body, and a base is arranged at the bottom end of the PCR analyzer body; the base comprises a circular shell, a cooling fan is installed in the shell, and four supporting rods are annularly arranged on the surface of the shell at equal intervals. According to the PCR analyzer disclosed by the utility model, the heat dissipation fan is arranged on the base, so that the bottom end of the PCR analyzer can be cooled, the problem that the bottom end of the PCR analyzer is close to a working table surface and is difficult to cool in a short time under long-time work is avoided, and meanwhile, a movable block can drive a supporting strip and a base plate to move by rotating a screw rod; the height of the base is adjusted conveniently, so that the overall height of the PCR analyzer can be adjusted according to the heights of different workbenches, the convenience and comfort of operation are improved, and the bottom end of the cooling fan can be far away from the workbenches, so that better heat dissipation is facilitated.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

An automated polymerase chain reaction (PCR) analyzer (110) for analyzing at least one sample (112) and a method of automated PCR analysis is disclosed. The automated PCR analyzer (110) comprises: - at least one controller (114); - at least one thermocycling apparatus (116) configured for subjecting the sample (112) to at least one temperature modulation controlled by the controller (114); and - at least one photometer (118) configured for detecting at least one optically detectable property of the sample (112), the photometer (118) comprising at least one excitation light source (120) configured for illuminating the sample (112) with excitation light (122), the photometer (118) further comprising at least one photodetector unit (124) configured for detecting light (123) emitted from the sample (112), the photometer (118) further comprising a movable filter cell (126) controllable by the controller (114), the movable filter cell (126) comprising a plurality of filter cell units (128), each filter cell unit (128) comprising at least one optical excitation filter element (130), at least one dichroic filter (132), at least one optical detection filter element (134) and at least one the aperture stop (174), wherein the photometer (118) comprises a working position for the filter cell units (128), wherein the movable filter cell (126) is configured for selectively positioning a selected filter cell unit (128) selected from the plurality of filter cell units (128) in the working position, wherein, in the working position, the selected filter cell unit (128) is positioned such that the excitation light (122) interacts with the optical excitation filter element (130), the dichroic filter (132) and the aperture stop (174) of the selected filter cell unit (128) and that the light (123) emitted from the sample (112) interacts with the dichroic filter (132), the aperture stop (174) and the optical detection filter element (134) of the selected filter cell unit (128).

The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.

The present application relates to a kind of regulation Catalpa bungei salt stress response key gene CbLTP63 and its application, belong to plant genetic engineering and biotechnology field.CbLTP63 gene nucleotide sequence is as described in Seq 1, coding amino acid sequence is as described in Seq 2.The present application relates to the cloning of Catalpa bungei salt stress response key gene CbLTP63;CbLTP63-GFP subcellular localization is positioned on cell membrane by identifying agrobacterium injection method;By qRT-PCR analysis, it is identified that the gene responds to salt stress at transcription level, is down-regulated by H2O2, ABA, SA;By transgenic identification, the gene is Catalpa bungei salt stress response negative regulation gene, and the salt tolerance of Catalpa bungei callus after gene silencing is significantly enhanced;Also related to the use of the gene regulation Catalpa bungei stress response.The present application discloses a kind of Catalpa bungei salt stress response key gene CbLTP63, and has important application value in the field of Catalpa bungei genetic engineering and clone forestry.

A device (1) for PCR analysis comprises a loading unit (2) having a first movement mechanism to move a sample holder receiving one or more samples from one zone to another between an entry zone and a loading zone, the device (1) comprising a second movement mechanism (5) to move a sample holder receiving one or more samples from one zone to another between an entry zone and a loading zone, the second movement mechanism is configured to move at least one sample between two zones selected from a list comprising the loading zone, a thermal and / or pressure zone of the thermal and / or pressure unit (3) and a scanning zone of the scanning unit (4). A method for controlling such an apparatus (1), and a corresponding computer program and computer-readable storage medium.

The application discloses a large white pig genome prediction method based on improved genome feature principal component regression, which is defined as GF_PCR. The method divides the traditional PCR method into two parts, performs simple regression on the genome feature part, and performs PCR analysis on the remaining SNPs. When the genome feature contains more causal mutations, the model can obtain higher genome prediction accuracy. At the same time, the genome prediction accuracy of the GF_PCR model for the 100Kg body weight day age trait is higher than that of the PCR model, and the reason is that the proportion of the causal mutation in the preselected genome feature is larger, and the GF_PCR further improves the genome prediction accuracy.

This invention provides a method for determining the minimum number of droplets required for droplet-based digital PCR analysis, relating to the field of PCR detection technology. The method includes: obtaining target parameters, including gene copy number, theoretical droplet number, target confidence level, and target error; and determining the minimum number of droplets corresponding to the target parameters based on a pre-established theoretical model relating droplet number to confidence level and error. The theoretical model relating droplet number to confidence level and error is based on the n-fold Bernoulli distribution theory and Chebyshev's inequality. This method theoretically determines the minimum number of droplets required for droplet-based digital PCR analysis, alleviating the problem of currently determining the minimum droplet number only empirically. It is easy to understand and implement, thereby improving the consistency and reliability of the analytical results.

This utility model relates to the field of medical device technology, specifically disclosing a cap-switching device and a PCR analysis device, including a carrier plate, a clamping mechanism, and a vertical moving mechanism. The clamping mechanism includes a support bracket, a first driving component, a pressing component, and a snap-fit ​​component. The support bracket is movably mounted on the carrier plate along the Z-direction, and the first driving component and the snap-fit ​​component are both mounted on the support bracket. The driving end of the first driving component is connected to the pressing component. The vertical moving mechanism is mounted on the carrier plate, and its driving end is connected to the support bracket. The cap-switching device of this utility model enables the opening and closing of reaction consumables, avoiding the difficulty of opening and closing caps caused by tilting of the tube caps. It is not only highly efficient but also prevents deformation of the tube body or the tube caps, thereby reducing the probability of errors and improving the accuracy and reliability of the tube caps being mounted on the tube body. It also reduces labor costs and the risk of contamination.

The present invention relates to a digital real-time PCR analysis method, and the purpose of the present invention is to provide a digital real-time PCR analysis method capable of digital real-time PCR analysis of a low-concentration or a high-concentration sample which is beyond an existing measurement limit, i.e., samples of a wide concentration range.