149 results about "Bioanalysis" patented technology

This disclosure provides methods for spatial profiling of biological analytes present in a biological sample. Methods include generating feature arrays using a master / copy format using recessed arrays, and methods for using such arrays. For example spatially-tagged analyte capture analytes can be used in spatial detection in methods to determine the location of analytes (e.g., proteins) in biological samples.

The invention belongs to the technical field of biological analysis and evaluation, and particularly relates to a stem cell differentiation process multi-modal image fusion and intelligent evaluation method, which comprises a stem cell differentiation process multi-modal image fusion method: on the basis of constructing a multi-modal near-physiological mechanics microenvironment, carrying out image analysis by adopting the multi-modal image fusion method; an intelligent evaluation method for the stem cell differentiation process: developing an intelligent evaluation method based on deep learning driving on the basis of constructing a multi-modal near-physiological mechanical microenvironment, and revealing a mechanical-epigenetic regulation mechanism; the synergistic application of the two solves the core observation and regulation problems in stem cell differentiation research, and provides an industrialized intelligent platform for regenerative medicine. According to the method, the data integrity can be improved, the resolution limitation is broken through, the experiment process is accelerated, accurate mechanism analysis can be realized, automatic decision making is promoted, and the research and development cost is reduced.

The invention relates to the technical field of environmental and biological analysis and detection, in particular to a method for extracting perfluorinated / polyfluorinated compounds based on confinement fluid. The method comprises the following steps: providing a multi-dimensional reticular nanopore material; growing mutually staggered carbon nanofibers on the surface of a substrate through a chemical vapor deposition method; loading a confinement fluid in a pore channel of the multi-dimensional reticular nano-pore channel material; contacting the material with a to-be-detected sample, and extracting; desorbing the extracted material by using a desorption solvent to obtain a desorption solution; and detecting the perfluorinated / polyfluorinated compounds in the desorption solution. A multi-dimensional net-shaped nano pore channel formed by mutual staggering of the carbon nanofibers provides a stable multi-dimensional micro-nano confinement space for confinement fluid. According to the structure, mass transfer and distribution of a target analyte from a complex matrix to a confinement fluid are greatly promoted, so that efficient enrichment of various perfluoro / polyfluoro compounds is realized, and the recovery rate and the detection sensitivity of the method are improved.

A microneedle device comprises a device structure, insertable to a living body and being formed with an opened niche at least partially surrounded by walls of micrometric heights above a base of the niche for allowing the niche to be filled with a blood sample upon the insertion. The device also comprises a biosensor configured to sense a bioanalyte in the blood sample and having a sensing element formed on the base. The thickness of the sensing element is less than the micrometric heights of the walls.

The invention belongs to the technical field of biological analysis and detection, and particularly relates to a photoelectrochemical-colorimetric dual-mode biosensor and application thereof in miRNA detection. According to the biosensor prepared by the invention, the bifunctional CuO-Cu2O nanoflower and the three-dimensional network g-C3N4 / AuNPs electrode are integrated, and an enzyme-assisted strand displacement signal amplification mechanism is combined, so that dual-mode collaborative detection of a target miRNA-21 is realized: in a photoelectrochemical mode, the detection limit reaches 0.35 fM (the linear range is 1 fM to 10 fM), and response is performed based on photocurrent polarity reversal; the colorimetric mode is as follows: the detection limit is 3.9 fM (the linear range is 10 fM to 10 nM), and TMB (Tetramethylbenzidine) color development visualization analysis is carried out. Single-mode interference is effectively eliminated through double-signal independent output and cross validation, and the method is suitable for high-reliability detection of trace miRNA (such as a breast cancer marker miRNA-21) in complex samples such as serum. According to the invention, the defects of insufficient sensitivity (pM level), poor anti-interference performance and high platform complexity in the prior art are overcome, and an efficient tool is provided for early diagnosis of cancers.

The invention relates to the technical field of oral medicine and biological information analysis, in particular to an evaluation system for oral microbiological analysis and decayed tooth risk prediction. The system comprises a sample collection module, a microorganism detection module, a data preprocessing module, a microorganism population dynamic analysis module, a multi-dimensional feature extraction module, a decayed tooth risk assessment module and a personalized suggestion generation module. By constructing microbial population evolution analysis based on a Lotka-Volterra competitive model, diversity dynamic change analysis based on Shannon entropy and evolution trend prediction based on a Lyapunov stability theory, the system deeply reveals a dynamic evolution law of an oral micro-ecosystem, and a multi-dimensional feature adaptive weighting mechanism based on mutual information is adopted, so that the dynamic evolution law of the oral micro-ecosystem is improved. Personalized accurate risk assessment is realized, a causal reasoning theory is introduced to generate personalized intervention suggestions with causal support, and the accuracy and the prevention effect of dental caries risk prediction are remarkably improved.

This invention provides a simple synthesis method for Fe-Ag / CMP nanopolymers with catalase-like activity and their application in hydrogen peroxide detection, belonging to the field of bioanalytical technology. The method of this invention includes the following steps: (1) preparing a 4-hydroxyethylpiperazine thiosulfonic acid (HEPES) standard buffer solution; (2) adding cytidine monophosphate (CMP) to the solution obtained in step (1) and stirring until the CMP is fully dissolved. Then, under vigorous stirring, ferric nitrate aqueous solution and silver nitrate aqueous solution are added sequentially, at which point the colorless solution turns pale yellow; (3) transferring the mixed solution obtained in step (2) to a centrifuge tube, centrifuging at 12000 rpm for 8 min to collect the precipitate, washing it three times with distilled water, and finally adding 4 mL of distilled water to obtain a 1 mg / mL Fe-Ag / CMP suspension. This invention utilizes CMP as a biological ligand and Ag... + Fe 3+ Simultaneous coordination, and the reducing amino groups on CMP can directly transfer Ag... + In situ reduction to silver nanoparticles (AgNPs) led to the one-step synthesis of a bimetallic Fe-Ag / CMP nanopolymer with hydrogen peroxide-like nanozyme activity, enabling the detection of hydrogen peroxide.

The invention discloses a cascade seven-core optical fiber sensor applied to folic acid detection and a preparation method of the cascade seven-core optical fiber sensor, and belongs to the technical field of optical fiber sensing and biological analysis detection. The sensor comprises a first single-mode optical fiber, a seven-core optical fiber section and a second single-mode optical fiber, a double-microsphere-cavity structure is arranged at the optical fiber connecting position to form a cascade interference optical path, and tapering is conducted on the middle portion of the seven-core optical fiber section to form a sensing area. According to the invention, mode matching is improved through the microsphere cavity, an evanescent field is enhanced through tapering, and high-sensitivity specific detection of folic acid is realized in cooperation with the folic acid binding protein biological functional layer fixed on the surface; the sensor obtains stable linear response to a folic acid standard solution in the range of 0-50 ng / mL, a clinical serum sample verifies that the detection result of the sensor is highly consistent with that of a chemiluminescence method, the sensor has good reusability, and the sensor has the advantages of being easy to prepare in structure, rapid in response, high in sensitivity, capable of being matched with a complex matrix and the like, and has good application prospects. The method has huge potential in clinical folic acid quantitative detection.

The invention relates to the technical field of biological analysis and detection, and particularly discloses a streptococcus mutans detection kit based on an anchored CRISPR-Cas12a enhanced paper-based micro-fluidic chip and a detection method. The detection kit comprises an RAA solution or RAA freeze-dried powder, magnesium acetate or a magnesium acetate solution, TMB or a TMB solution, an RAA forward primer, an RAA reverse primer, a cell lysis buffer, a buffer system and a paper-based micro-fluidic chip. The detection method comprises the following steps: establishing a standard curve and detecting a sample. The detection kit is used for detecting the content of streptococcus mutans and has the characteristics of high efficiency, rapidness, specificity, sensitivity, portability and the like, and the method is simple and convenient to operate, low in detection cost and capable of being applied to on-site detection of streptococcus mutans.

The invention concerns a remote medical-diagnosis system comprising 1) a self-sampling medical device, 2) at least one reception device, 3) at least one analysis device; and 4) a server configured to register a subject and his biological analysis request, to record the data related to the self-sampling medical device and its associated biological sample, to select at least one biological test to be realized on the self-sampling medical device, to request the at least one analysis device for performing the at least one selected biological test; to receive the data corresponding to the results of the at least one selected biological test performed by the at least one analysis device on the self-sampling medical device and produce and transmit to the subject a report comprising these results and / or eventually request a medical appointment for the subject.

The application discloses a kind of based on digital droplet microfluidic multi-sample flux multi-index detection device and application thereof, belong to electromechanical device, microfluidic chip and immunoprotein analysis field.The device includes droplet microfluidic equipment and immunoprotein analysis chip component, droplet microfluidic equipment is used to drive multiple droplets, mainly divided into three major systems: power supply system, computer operating system and chip clamp system, immunoprotein analysis chip component includes biological analysis substrate and microarray chip.The present application drives micro liquid sample by digital droplet microfluidic technology, realizes the multi-index detection of automated immunoprotein analysis chip.The detection device of the present application has a series of advantages such as rapidity, independence, low consumption, automation, function integration and multi-index detection, and is expected to become the most fruitful rapid diagnostic device for improving tumor diagnosis positive rate.

The application belongs to the field of biological analysis, and provides a method for precise metabolic flow analysis of TCA cycle based on isotope tracing technology combined with high-resolution mass spectrometry. By optimizing mass spectrometry parameters and liquid chromatography method, one injection within 6 min is realized, and high-throughput, high-sensitivity and high-resolution detection is realized on 11 biomarkers related to TCA cycle metabolic pathways. At the same time, the application innovatively uses LC-MS / MS to decipher 13 C the stepwise position-specific transfer from glucose to subsequent metabolites by glycolysis and TCA cycle, thereby calculating 13 C the integrated steady-state analysis of key metabolic rates of position-specific transfer from sequential precursors to their products (pyruvate dehydrogenase, beta-oxidation, pyruvate carboxylase, isocitrate dehydrogenase and pyruvate cycle). This technology has wide applicability and can potentially characterize mitochondrial metabolism of any tissue or cell.

The invention discloses construction and application of a biosensor for triggering controlled release based on adjacent induced DNA strand displacement, and belongs to the technical fields of electrochemical luminescence, biological analysis and nano science. According to the invention, by integrating adjacent induced DNA strand displacement and controlled release strategies, the ultra-sensitive self-closing dual-mode biosensor taking the Au-Ag nanocluster as a luminous body is developed, and the ultra-sensitive self-closing dual-mode biosensor is used for trace detection of neuron-specific enolase. Due to the synergistic characteristic between the double metals, the Au-Ag nanocluster has high electrochemical luminescence (ECL) efficiency. Meanwhile, the reduction of S2O8 < 2-> is catalyzed by cyclic conversion between Fe < 2 + > / Mn < 3 + > and Fe < 3 + > / Mn < 4 + >, and a large amount of SO4 <-> is generated for ECL emission. The specific binding between the target antigen and the antibody can drive DNA strand displacement to open a blocked hole, so that the electrochemical probe falls off and the probe is quenched to release, the self-closing of an electrochemical signal and an ECL signal is realized, the detection sensitivity and accuracy of the sensor are effectively improved, and the sensor is suitable for immunoassay of various biomarkers.

The invention relates to the technical field of bioanalytical chemistry, in particular to a gold nanoparticle detection method for realizing multi-microorganism detection. The preparation method comprises the following steps: synthesizing gold nanostars with rich tip structures by adopting a surfactant-free reduction method, taking the gold nanostars as a high-activity SERS substrate, coding by utilizing different Raman reporter molecules, and coupling a specific antibody through a heterobifunctional PEG (Polyethylene Glycol) connecting arm to prepare the multiple SERS immune nano-label; meanwhile, functional immunomagnetic nanoparticles are constructed and are used for capturing and enriching target bacteria from a complex sample. The detection process adopts a magnetic enrichment-compounding-SERS spectrum reading mode to form a magnetic bead-bacteria-SERS label compound, and a portable Raman spectrometer is used for collecting signals and combining a deconvolution algorithm to realize synchronous qualitative and quantitative analysis of various pathogens. The method disclosed by the invention has the advantages of high detection sensitivity, high detection speed, strong matrix interference resistance, no spectral crosstalk due to multiple detection and the like.

The application belongs to the technical field of nanomaterials, and provides ascorbic acid oxidation mimetic enzyme and preparation thereof. The application adopts a methanol solution of zinc nitrate, copper acetylacetonate and 2-methyl imidazole to perform a hydrothermal reaction, and obtains the ascorbic acid oxidation mimetic enzyme by oxygen isolation calcination of a hydrothermal product. The copper-nitrogen-carbon nanomaterial obtained by the application has a rhombohedron structure, can specifically oxidize ascorbic acid, and can be applied to ascorbic acid content detection, oxidized ascorbic acid preparation and dehydroascorbic acid preparation as ascorbic acid oxidation enzyme mimetic enzyme. The nanomaterial can maintain ascorbic acid oxidation mimetic enzyme activity under acid, alkaline and high temperature conditions; the steady-state kinetic parameter (Michaelis constant is 0.012 mM) is better than that of natural ascorbic acid oxidation enzyme, and the ascorbic acid oxidation mimetic enzyme activity of the material has a broad application prospect in the fields of nanozyme, nanotechnology and biological analysis detection.

The invention discloses an amino acid L / D type enantiomer separation and quantitative detection method based on LC-MS, and belongs to the technical field of biological analysis. Aiming at the problems of difficulty in separation of amino acid L / D type enantiomers in human serum, low detection sensitivity, complex analysis process and the like, the method disclosed by the invention is simple in pretreatment, short in instrument collection time, high in chiral enantiomer separation degree, small in matrix effect, high in sensitivity, low in cost and convenient for large-scale clinical sample detection in a laboratory; the method not only can make up the limitation that only the L type is detected at present, but also can provide a key analysis method for early warning of diseases, drug effect and toxicokinetics research and precise medical evaluation.

The invention discloses a probe BBM for anchoring OPA1 protein and application of the probe BBM, and belongs to the technical field of biological analysis. Based on the characteristic that the OPA1 protein is rich in hydrophobic amino acid residues and polar amino acids, the probe BBM capable of generating multiple interaction forces with the OPA1 protein is designed by reasonably introducing hydrophobic groups and polar groups. Theoretical calculation and experimental results (including OPA1 knock-down and overexpression) show that the probe realizes efficient enrichment in a mitochondrial inner membrane region. By using the unique probe, the hydrogen peroxide induced mitochondrial inner membrane viscosity change is systematically researched, and the myocardial cell oxidative damage is further confirmed to be realized by a p53 protein mediated apoptosis pathway. The method provides a reliable means for accurately evaluating the oxidative stress of the mitochondrial inner membrane, and has important promotion significance for the development of the cardiovascular research field.

This document describes medical systems for detecting biological analytes. For example, this document describes sensors for the continuous monitoring of biological analytes, such as glucose and / or lactate, in aqueous solutions and body fluids (e.g., blood) based on a readout of fluorescence or luminescence signals.WO

This document discloses an apparatus comprising: an X-ray source (101); an X-ray detector (100); wherein the X-ray source (101) is configured to guide an X-ray beam (102) at a grazing angle (108) to a surface (104), at which the X-ray beam (102) is totally externally reflected by the surface (104); wherein a first bioanalyte (910) is fixed to the surface (104), and a first element (911) is attached to the first bioanalyte (910); wherein the X-ray beam (102) is capable of eliciting the emission of characteristic X-rays (109) of the first element (911); wherein the X-ray detector (100) is configured to receive the characteristic X-rays (109) of the first element (911), but not to receive the X-ray beam (102) reflected by the surface (104).

The invention provides a fluorescent probe as well as a preparation method and application thereof, and belongs to the technical field of biological analysis and detection. The fluorescent probe disclosed by the invention has a structure as shown in a formula I; the invention also provides a preparation method of the fluorescent probe and application of the fluorescent probe in cell activity detection or preparation of a cell activity detection kit. The fluorescent probe provided by the invention can reflect the cell state by migrating organelles, realizes dual-channel imaging, and can meet the actual requirements of cell activity detection.

The invention relates to a method for quantitatively determining biological analytes in an aqueous solution in the presence of one or more functionalised surfaces, wherein the aqueous solution comprises at least one type of biological analyte and at least one type of fluorescene marker, characterised in that the quantity and / or concentration of the biological analyte or analytes is determined by measuring the florescence emission of the unbound fluorescence markers, as well as to a device for carrying out said method.

The present invention relates to a method for bioanalysis, in particular, to a method for generating electrochemiluminescence signal at low voltage to achieve high signal-to-noise ratio and improved concentration-signal response relationship, thereby improving electrochemiluminescence (ECL) immunoassay performance.

The invention belongs to the technical field of biological analysis, and relates to a method for performing mass spectrum imaging analysis on lung tissues and application. In order to solve the problem that the structural integrity is difficult to maintain in the lung tissue freezing and slicing process, a liquid shaping agent is adopted to conduct internal perfusion on fresh lung tissue, the airway and the pulmonary alveoli are filled, and the shaping agent supports the lung tissue to maintain the natural state in the subsequent operation after being solidified at low temperature. The prepared lung tissue frozen section can be used for mass spectrum imaging analysis and research.

The invention discloses a testing device of a biological analyzer and a biological analysis kit. Wherein the biological analyzer is provided with a measuring end and a first communication circuit, and the testing device comprises a device body, and a main control circuit, a second communication circuit and a biological parameter equivalent circuit which are arranged in the device body; the second communication circuit is in communication connection with the first communication circuit and is electrically connected with the main control circuit; the main control circuit is used for adjusting circuit parameters of the biological parameter equivalent circuit for multiple times under the condition that the second communication circuit is in communication connection with the first communication circuit; after each adjustment, the biological analyzer is controlled to perform a measurement action based on the circuit parameters of the biological parameter equivalent circuit through the second communication circuit and the first communication circuit; and the counting module is also used for counting the number of times of measurement until the biological analyzer does not transmit the measurement completion signal when receiving the measurement completion signal transmitted by the biological analyzer. The invention aims to improve the efficiency of testing the upper limit of the storage times of the test result of the biological analyzer.

The application belongs to the field of sensing detection, and relates to a method for rapidly detecting sulfur ions in a double channel, which uses tin telluride nanometer enzyme as a double channel colorimetric probe, detects sulfur ions through two channels, one channel is a specific brown change caused by the binding energy between tin ions and sulfur ions, and is used for detecting sulfur ions with a concentration of greater than or equal to 100 muM; the other channel is a TMB colorimetric method for detecting sulfur ions with a concentration of less than 100 muM; it is found for the first time that the double channel colorimetric probe tin telluride nanometer enzyme can be used for detecting sulfur ions, has high selectivity for sulfur ions, has super-high sensitivity, and is not easily disturbed by other ions; specific brown changes caused by the binding between tin ions and sulfur ions can realize the visualization of sulfur ions in a solution and the rapid, accurate and wide-range concentration determination of photoelectrochemistry; therefore, the method provides a new idea for a 'one platform and multiple channels' detection method of chemical and biological analysis.

A biological analyzer's ability to report mean corpuscular volume (MCV) values may be tested using a method which includes providing a control sample, capturing measurements of particles, and reporting a set of MCV values based on the measurements. In support of such a method a sample may be provided which comprises different subsets of control particles which each have their own corresponding size range. These size ranges may be set so that no more than one of the subsets would be occluded by red blood cells (RBCs) that may also be present in the control sample, so that measurements of the remaining subsets may be used in determining the MCV value(s) to be reported.

The application discloses an immunological analysis method based on high-molecular amplification signal amplification and belongs to the technical field of biological medicines. The application combines protein-high-molecular conjugates with biological analysis together, and successfully develops a method for protein quantitative analysis. On the basis of taking silica microspheres as a solid-phase carrier material, target analysis antigen protein molecules are coated in advance, specific recognition reaction of antigen and antibody is utilized, antibody IgG-I macromolecules grafted with a photo initiator in advance are indirectly fixed on the surface of the microspheres, and a "competition" immunological detection method is constructed. The method is a rapid, simple, inexpensive and enzyme-free cascade amplification detection technology, and has a wide application prospect.

According to the light-induced photoelectrochemical integrated device provided by the invention, electrochemical luminescence and PEC signal simultaneous output are realized in a single sensing system for the first time, and dual-mode simultaneous quantification of markers in serum is realized. The preparation method comprises the following steps: preparing and screening an ECL / PEC material pair with matched wavelength: luminol-Au / Bi2WO6, and successively assembling Bi2WO6 and luminol-Au labeled probes on an indium tin oxide electrode by utilizing a layer-by-layer self-assembly mode; when a voltage is applied, the luminol-Au emits a strong ECL signal and triggers the Bi2WO6 to generate an obvious light current. A marker miRNA-21 is taken as a model, recognition molecules with a hairpin structure are introduced into a system, and sensitive and accurate dual-mode analysis of the miRNA-21 is realized by adopting an enzyme-assisted amplification method. The detection method provided by the invention not only can realize ECL-PEC dual-mode simultaneous detection of the target object, but also has relatively high universality, and a new example can be provided for precise biological analysis by replacing and recognizing molecules through the sensor.

The invention pertains to the technical fields of bioanalytics and agriculture, and relates to a method and to an assembly for in-ovo sexing of bird eggs. The aim of the present invention is to provide a method and an assembly by means of which in-ovo sexing of bird eggs is achieved with a stable predictive accuracy of the sex and which are simple and cost-effective. The aim is achieved using a method and an assembly in which a fertilised bird egg incubated for 3 to 9 days is irradiated with at least two light-emitting laser beam sources with different excitation waves after the shell is opened, the captured and processed fluorescence scattering spectra and Raman scattering spectra being used, as complementary spectra associated with the different excitation wavelengths, to determine a classification value, and the sex being determined by comparing the determined classification value with an intrinsically determined threshold value. The method and the assembly can be used for sexing farm poultry.