Preparation method of long carbon chain dibasic acid

A technology of long-chain dibasic acid and production method, applied in the field of bioengineering, can solve the problems of slow development of downstream products of long-chain dibasic acid, backward production technology and equipment, and inability to meet customer needs, etc. The effect of cultivation process, shortened production cycle and low production cost

Active Publication Date: 2011-05-18
CATHAY R&D CENT CO LTD +2
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] 2. The production technology and equipment are backward, and the product grade is low
[0011] 4. The product is single, and the production of long-chain dibasic acid series products has not been formed, which cannot meet the needs of the market
[0012] 5. Insufficient investment in research and development, slow development of downstream products of long-chain dibasic acids
[0013] 6. The detection technology is backward and cannot meet the needs of customers

Method used

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  • Preparation method of long carbon chain dibasic acid
  • Preparation method of long carbon chain dibasic acid
  • Preparation method of long carbon chain dibasic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Fermentation control conditions:

[0048]Inoculum size: 8%, tank temperature: 25°C, ventilation ratio: 1:0.3vvm, tank pressure: 0.08Mpa, pH: 4.0 before adding alkanes, 6.5 after adding alkanes, culture time: 120 hours.

[0049] Feeding control parameters:

[0050] Alkanes: when the cell growth concentration is greater than (OD 620 ) 0.8, start to add cell regulator and alkane, add cell regulator once and add, control concentration of alkane in the fermentation broth is 3%, stop feeding 20 hours before the end of fermentation.

[0051] Extraction process of fermentation broth:

[0052] The fermented liquid is heated to 70°C by a steam heater and put into the fermented liquid storage tank. Afterwards, add liquid alkali to adjust the pH of the fermented liquid to 8.5 ~, separate the bacteria by membrane filtration, add 0.1% activated carbon to the dibasic acid clear liquid, decolorize at 50°C for 40 minutes, and then filter to obtain the decolorized clear liquid and aci...

Embodiment 2

[0056] Fermentation control conditions:

[0057] Inoculum size: 10%, tank temperature: 30°C, ventilation ratio: 1:0.5vvm, tank pressure: 0.10Mpa, pH: 5.0 before adding alkane, 7.5 after adding alkane, culture time: 140 hours.

[0058] Feeding control parameters:

[0059] Alkanes: when the cell growth concentration is greater than (OD 620 ) 0.8, start to add cell regulator and alkane, add cell regulator once and add, control concentration of alkane in the fermentation broth is 7%, stop feeding 20 hours before the end of fermentation.

[0060] Extraction process of fermentation broth:

[0061] The fermented liquid is heated to 70-90°C by a steam heater and put into the fermented liquid storage tank. Afterwards, add liquid alkali to adjust the pH of the fermentation broth to 9.5, separate the bacteria by membrane filtration, add 0.5% activated carbon to the dibasic acid clear liquid, decolorize at 65°C for 80 minutes, and then filter to obtain the decolorized clear liquid and ...

Embodiment 3

[0065] Fermentation control conditions:

[0066] Inoculum size: 20%, tank temperature: 35°C, ventilation ratio: 1:0.7vvm, tank pressure: 0.12Mpa, pH: 6.0 before adding alkane, 8.5 after adding alkane, culture time: 160 hours.

[0067] Feeding control parameters:

[0068] Alkanes: when the cell growth concentration is greater than (OD 620 ) 0.8, start to add cell regulator and alkane, add cell regulator once and add, control concentration of alkane in the fermentation broth is 10%, stop feeding 20 hours before the end of fermentation.

[0069] Extraction process of fermentation broth:

[0070] The fermented liquid is heated to 70-90°C by a steam heater and put into the fermented liquid storage tank. Afterwards, add liquid alkali to adjust the pH of the fermentation broth to 10.5, separate the bacteria by membrane filtration, add 1.0% activated carbon to the dibasic acid clear liquid, decolorize at 80°C for 120 minutes, and then filter to obtain the decolorized clear liquid a...

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Abstract

The invention relates to a preparation method of a long carbon chain dibasic acid. C11 and upper alkane is used as a substrate to produce a corresponding long carbon chain dibasic acid product through the conversion of the substrate into the long carbon chain dibasic acid by using a microbial fermentation method as well as the extraction and the separation of fermentation liquid and the refining processes of a crude product of dibasic acid. Through technological innovation and process innovation, the invention researches a new preparation method of the long carbon chain dibasic acid, greatly decreases the production cost of the long carbon chain dibasic acid, improves the yield and the product quality of the long carbon chain dibasic acid, can produce C11 and upper long carbon chain dibasic acid, finally solve the bottleneck problem restricting the rapid development of the long carbon chain dibasic acid, and form the industrialized scale and the technological predominance. The long carbon chain dibasic acid produced by using the bioanalysis provided by the invention has the advantages of high acid generation level, low production cost, good product quality, complete variety, and the like, the prepared long carbon chain dibasic acid product has high single acid content, good light transmission and high thermal stability, can meet the requirements of different clients, and can be used for producing high-grade spices, high-performance engineering plastics, high-temperature dielectric medium, high-grade hot-melt adhesive, coldness-resistant plasticizer, high-grade lubricating oil, high-grade paint, coating, and the like. The invention greatly widens the development space of the downstream products of the long carbon chain dibasic acid.

Description

technical field [0001] The invention belongs to the field of bioengineering, and relates to a method for producing long-carbon-chain dibasic acid series products by using a biological method. Background technique [0002] Long chain dicarboxylic acids (Long chain dicarboxy acids) refer to aliphatic dicarboxylic acids (DC for short) containing more than 10 carbon atoms in the carbon chain. n ), including saturated and unsaturated dicarboxylic acids, is a class of fine chemical products with important and extensive industrial uses. , high-grade lubricants, high-grade paints and coatings and other important raw materials. [0003] The production methods of long carbon chain dibasic acids include chemical synthesis and bio-fermentation methods. Foreign countries mainly use chemical synthesis methods. This method mainly uses butadiene as raw material and is produced by chemical synthesis methods. The process is complicated and the synthesis conditions are harsh. It takes 200 Hi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/74
Inventor 曹务波王志洲陈远童葛明华傅深展
Owner CATHAY R&D CENT CO LTD
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