The invention discloses a preparation method of a near-
infrared two-region
BODIPY dye for marking a
biological membrane, and relates to the technical field of biological medicines. The invention aims to solve the technical problems of difficult synthesis, more steps and slower
cell membrane labeling speed of the existing
cell membrane labeling fluorescent molecules. The method comprises the following steps: by taking a near-
infrared two-region aza-
BODIPY dye as a main body structure, introducing an
alkyl chain and hydrophilic
sulfonate or
quaternary ammonium salt on a molecular structure, and modifying and
coupling to obtain BMP1, BMP2 and BMP3. According to the near-
infrared two-region aza-
Bodipy dye and the preparation method thereof, a series of BMP1, BMP2 and BMP3 are synthesized, and the near-infrared two-region aza-
Bodipy dye is excellent in water
solubility. The
fluorescence brightness of the three materials before and after being combined with the lipidosome reaches more than 10 times, and the BMP3 material has more excellent hydrophilic performance, does not need DMSO to assist
dissolution, can be better combined with the lipidosome, has larger change in
fluorescence intensity before and after, and can better become a marking dye of a
biological membrane structure material. The near-infrared two-region
BODIPY dye prepared by the preparation method disclosed by the invention is used for marking a
biomass film.