The invention relates to the technical field of cell automation augmentation and culture instrument, in particular relates to an automation augmentation and culture system of induced pluripotent stem cells, which comprises a cell automation augmentation and culture system, an operation room and a control system which are mutually connected, wherein, the cell automation augmentation and culture system comprises a culture box and a culture box control room connected with the culture box; the culture box comprises at least one culture device for fixing a culture container fixed block of a culture container, a culture container cover automatic opening and closing system, a culture container automatic popping and closing system, a digital temperature system and at least one sensor; the culture box control room comprises an air flow purification and induction system and a culture box digital power system; and the operation room comprises a bar code automatic entry system, an liquid automatic replace system and a cell on-line observing system. By utilizing the system provided by the invention, a somatic cell can be automatically induced into a pluripotent stem cell, and the system can be used for stem cell research and clinical stem cell treatment.