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2033 results about "Incubator" patented technology

Incubator is a device used to grow and maintain microbiological cultures or cell cultures. The incubator maintains optimal temperature, humidity and other conditions such as the CO (CO₂) and oxygen content of the atmosphere inside. Incubators are essential for a lot of experimental work in cell biology, microbiology and molecular biology and are used to culture both bacterial as well as eukaryotic cells.

Environmental control incubator with removable drawer and robot

InactiveUS20060177922A1Ultimate compactnessStable and reliable controlBioreactor/fermenter combinationsHeating or cooling apparatusRobot positionMicro plate
An incubator for storing micro-plates or micro-tubes comprises a handling robot positioned between shelves or drawers containing micro-titer plates or other containers useful for biological based reactions. The advantages of this configuration are the ultimate compactness of the system and increased speed or reliability achieved with more than one robot being able to access the same plate or tube. Alternative embodiments standardize the spacing and configuration of a robot track and a shelf track such that a shelf and a robot are interchangeable in a track.
Owner:AGILENT TECH INC

Neonate's incubator and MRI docking-station

A docking-station useful for providing a neonate predefined, continuous, stabilized and non-interrupted life-support environmental conditions, comprising of: (i) a neonate incubator, having at least one first opening and a life support system, and (ii) an imaging-device, having a scanning chamber with at least one second opening. The docking-station is configured such that said neonate incubator first opening and said scanning chamber second opening are juxtapose-able so as to reversibly hermetically communicate; thereby providing said neonate predefined, continuous, stabilized, non-interrupted life-support environmental conditions during the entire process of scanning.
Owner:ASPECT IMAGING

System for in-vitro fertilization with spermatozoa separated into X-chromosome and Y-chromosome bearing populations

An IVF system for successfully utilizing spermatozoa separated into X-chromosome bearing and into Y-chromosome bearing population for insemination. The IVF system includes fertilization medium that can shorten the time from insemination to cleavage and a portable incubator for the transportation of maturing oocytes and inseminated oocytes comrprising a straw (19) and an incubation element (20) that can be sealed with a cap (22).
Owner:XY

Incubator with double glazed wall and methods thereof

An incubator with a double glazed wall that promotes regulation of sound pressure levels and temperature levels the incubator, using an insulated glass unit. The canopy of the incubator has at least one insulated glass unit. This unit includes at least one first inner glass and at least one second external glass. The inner and external glasses are spaced by a spacer, and are characterized by an inner atmosphere confined within the canopy and an external atmosphere.
Owner:ASPECT IMAGING

Mrd assembly of scanner and cart

InactiveUS20160081582A1Limit excess handlingEliminate needBaby-incubatorsOperating tablesResonanceEngineering
A magnetic resonance system (MRS), including a magnetic resonance device (MRD), comprising an open bore, the MRD at least partially contained in an envelope comprising in its circumference at least one recess; and, a cart made of MRI-safe material, comprising a base and at least one incubator above the base. The MRS is operative in a method of magnetic resonance imaging of neonates, comprising the steps of obtaining the MRS, the incubator is accommodated by a neonate; and inserting at least a portion of the cart into the MRD such that at least a portion of the incubator is inserted into the open bore and at least a portion of the base into at least one recess.
Owner:ASPECT IMAGING

Automated apparatus and method of cell culture

The invention provides an automated apparatus of cell culture having tanks of culture medium, of growth factors and of cells to be cultured, an incubator having a thermostated enclosure which houses a cell culture vessel, and control computer system. A supporting and agitation device of the culture vessel is provided in the enclosure, and the culture vessel is formed by a bag having at least one inlet port connected to the tanks and one outlet port connected devices for harvesting and storage of the cells after culture, these harvesting and storage devices and tanks being located outside the enclosure and being connected to the cell expansion bag ports by conduits which together with the cell expansion bag form a preassembled module passing through a wall of the enclosure.
Owner:CELLPROTHERA

Livestock incubator

A portable livestock incubating device is provided formed of a rectangular container having and incubating volume forming an incubating chamber defined by the zone within the incubating volume. Each each end wall further includes an access door hingedly attached to an upper portion above a clean out door for providing ingress and egress of livestock. Each access door has an exhaust vent located at an upper portion whereby heated air, generated via an electric heater within said the incubating chamber exhausts therethrough. A base with wheels mounted thereon for facilitating transportation of the portable livestock incubating device.
Owner:BOGNER DANIEL G +1

Artificial breeding method and incubator for shatang carp

The present invention is artificial breeding method and incubator for Shatang carp. The artificial breeding method includes the steps of parent fish breeding, spawning and fertilization, hatching and carp fry culturing. After intensified parent fish breeding, temperature control, flow water stimulation and other method are taken to promote the synchronous spawning and fertilization of parent fishes, the fertilized eggs on tiles are sterilized and hatched in a special hatching device, and the hatched carp fries are cultured in a special cement pond. The artificial hatching device is one rectangular hatching tank with shield, ventilating water circulating pump, water level and water flow controlling mechanism. The technology of the present invention is suitable for large scale production of Shatang carp fry.
Owner:ZHEJIANG INST OF FRESH WATER FISHERIES

In-Vitro fertilization systems with spermatozoa separated into X-chromosome and Y-chromosome bearing populations

An IVF system for successfully utilizing spermatozoa separated into X-chromosome bearing and into Y-chromosome bearing population for insemination. The IVF system includes fertilization medium that can shorten the time from insemination to cleavage and a portable incubator for the transportation of maturing oocytes and inseminated oocytes comprising a straw (19) and an incubation element (20) that can be sealed with a cap (22).
Owner:XY

Incubator comprising a shaking device

An incubator (1) with an incubation chamber (2), a shaking device (4) which can be driven by a drive unit and which is used to shake receptacles (5) that can be placed in the incubation chamber (2) and that contain cell cultures, and a device chamber (3) which adjoins the incubation chamber (2) and which accommodates at least parts of the shaking device (4) protruding into the incubation chamber (2), wherein the shaking device (4) has a base plate (8) which seals off the incubation chamber (2) relative to the device chamber (4) and which, on its inner face (9) directed toward the incubation chamber (2), has a shaking table (11) that can be moved in a horizontal plane by a drive arm (10), wherein a motor (34) is arranged on the outer face (37) of the base plate (8) directed away from the inner face (9), which motor (34) drives a drive shaft (14) that is rotatably mounted in the base plate (8) and that is operatively connected to the drive arm (10).
Owner:SARTORIUS STEDIM BIOTECH GMBH

Incubation system with low temperature enclosure

An incubator is fitted with an enclosure which surrounds the incubator. A cold air generation system supplies relatively cold air to a space formed between enclosure and the incubator. The system supplying the cold air can be embodied in a cart or base upon which the incubator sits. The enclosure is also placed on the base in a manner such that it substantially envelops the incubator. The top of the cart has openings for egress of cold air and return of air so that air may circulate in a closed loop. The incubator, cold air supply system and enclosure present a solution for incubating samples in a nominal 20-25° C. temperature environment. The enclosure, which is preferably insulated, may include a void or opening which exposes doors or other access device of the incubator, allowing direct access to the samples in the incubator while the rest of the incubator is enveloped by the enclosure.
Owner:BIOMERIEUX INC

A cold storage transport incubator

The invention discloses a cold-storage transport incubator, which comprises a box body and a box cover matched with the box body, the inner walls of the box body and the box cover are provided with a polyurethane foam plastic layer, and the polyurethane foam plastic layer There is a layer of vacuum heat insulation board inside the layer, and the polyurethane foam plastic layer and the vacuum heat insulation board form a composite heat insulation structure. The thickness of the polyurethane foam plastic layer is 6-30 mm, and the thickness of the vacuum insulation panel is 5-10 mm. The surface of the polyurethane foam layer or the vacuum insulation board located on the inner wall of the incubator is covered with a protective layer. Adopting the cold storage type transportation incubator of the present invention, since the vacuum insulation board with excellent heat insulation effect is provided in the polyurethane foam plastic layer, the thickness of the polyurethane foam plastic layer can be greatly reduced, so that the heat preservation box with the same shape can The effective volume of the box is greatly improved, the weight of the incubator is also reduced to a certain extent, and the heat preservation effect is also better.
Owner:BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES +1

Coal rock porosity, permeability and electroacoustic stress-strain combined measuring device under overburden pressure and heating

The invention relates to a coal rock porosity, permeability and electroacoustic stress-strain combined measuring device under overburden pressure and heating. The coal rock porosity, permeability and electroacoustic stress-strain combined measuring device is provided with an incubator, wherein a supporting rod, a comprehensive gripper, a pushing platform, a cushion block and a coal sample are arranged in the incubator, the upper part of the comprehensive gripper is provided with an experimental oil pipe, the experimental oil pipe is inserted into the coal sample, the upper part of the experimental oil pipe is sequentially connected with an electromagnetic valve e, a standard chamber, a pressure gauge b, an electromagnetic valve c, a barometric regulator and a gas booster pump, an inlet of the gas booster pump is communicated with a gas cylinder through a lead wire, and the upper part of the experimental oil pipe is sequentially connected with a pressure gauge c, an electromagnetic valve d, a water pressure regulator, a liquid booster pump and a water tank. The coal rock porosity, permeability, and electroacoustic stress-strain combined measuring device can be used for effectively simulating the high temperature and high pressure geological environment under deep complicated formation conditions to obtain the porosity, the gas-water relative permeability, a stress-strain curve, the resistivity and the acoustic velocity of the coal rock sample under the same experimental conditions, thus effectively saving the sample, improving the accuracy and the comparability of experimental data, and bringing the great convenience for scientific research.
Owner:CHINA UNIV OF MINING & TECH

Device and method for hatching embryos of Chinese softshell turtles

ActiveCN102986590AOvercoming mildewOvercome the disadvantages of breeding bacteriaAnimal husbandryAnimal scienceEmbryo
The invention relates to a device and method for hatching the embryos of Chinese softshell turtles. The device comprises an incubator, an incubation plate and a collecting box and is characterized by further comprising a temperature sensor, a humidity sensor, a temperature control device, a humidifying device and a ventilation device. After the device is adopted, fertile eggs can be automatically hatched in a completely naked state; the device is high in hatching efficiency; and the defects that the fertile eggs are mildewed, the embryos die and the like caused by medium incubation in the prior art can be avoided.
Owner:ZHEJIANG WANLI UNIV

Incubator deployable multi-functional panel

An incubator including a plurality of panels. At least one of the panels, or portion thereof, is a multi functional panel that is reversibly connected to at least one of the plurality of panels. The incubator can be opened and closed. In a closed configuration, the incubator sealingly encloses an internal environment. In an open configuration, the multi-functional panel is deployable as a countertop.
Owner:ASPECT IMAGING

Single cell separation method based on droplet micro-fluidic chips

The invention provides a single cell separation method based on droplet micro-fluidic chips. The method concretely comprises the following steps: A, a single cell suspension flows into a dispersed phase inlet channel from a dispersed phase inlet; B, an oil phase liquid flows into a continuous phase inlet channel from a continuous phase inlet; C, the above two phases merge to form droplets enclosing single cells, the droplets flow over a droplet capturing unit with the generation of a large number of droplets in a liquid storage pool, and stand for 2-5 min, and the superfluous droplets are sucked out when the droplets slowly settle into the droplet capture unit; and D, the droplet chips which capture the single cells are cultured in a 37 DEG C incubator, then DAPI is added to carry out nuclear staining, and the single cell capture rate is detected. The method has the advantages of simplicity and rapidity in operation, small use amounts of the cells and reagents, low experiment cost, high integration and wide application range.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI

Method for feeding lepidopteron

The invention discloses a method for feeding lepidopteron. In the feeding process, a tray type net cage is used as a main feeding tool. The method comprises steps of collecting eggs, sterilizing, hatching larvae from the eggs, feeding the larvae, feeding pupas, feeding imagoes and the like, wherein gauze or plastic ropes are used as an oviposition substrate for collecting the eggs; the eggs are sterilized by using a formaldehyde solution with the volume concentration of 3-4%; the eggs are hatched and fed in an illumination incubator; the larvae are sterilized by using a sodium hypochlorite solution with the volume concentration of 8-12%; pupas feather in the tray type net cage; the feathering imagoes are continuously fed in the net cage and the eggs are collected and sterilized. The tray type net cage is simple and convenient to operate, the insects are effectively prevented from escaping and the cleaning can be carried out at any time; in the feeding process, eggs and larvae are disinfected by using different disinfectants, the disinfection effect is thorough and gentle, the bacteria are effectively prevented from mass propagation, and the hatchability of the eggs, the feathering rate of pupa are also improved, the hatchability of the eggs can be 95-96%, and the feathering rate of pupa can be 98%.
Owner:QINGDAO AGRI UNIV

Method for identifying rice blast resistance of rice

A method for identifying rice blast resistance of rice comprises the steps of scissoring three rice stems in a large bract period from a rice growing field, bringing the rice stems back to a laboratory, scissoring 2cm from the upper portion of each stem section and 5-6cm from the lower portion of each stem section to obtain one rice stem with the whole length of approximate 7-8cm, putting the three rice stems into culture dishes with two layers of filter paper, adding sterile water of 1mL into each culture dish, washing cultivated rice blast fungus spores with the sterile water, obtaining concentration of 106 pcs / ml, dropping spore suspending liquid of 8 muL to stem section sites of each rice stem through a liquid moving gun, putting the culture dishes into a illuminating incubator at the temperature of 26 DEG C, wrapping the culture dishes with transparent films to keep humidity in the culture dishes, cultivating for ten days in light and dark alternating modes to survey disease conditions, dividing survey disease levels into three levels, regarding the first level as disease-resistant cultivars, regarding the second level as susceptible cultivars, and regarding the third level as highly susceptible cultivars. The method optimizes the concentration and inoculation amount of inoculated rice blast fungus spores and establishes simple, convenient and accurate grading standards.
Owner:INST OF PLANT PROTECTION JIANGXI ACAD OF AGRI SCI

Morchella esculenta cultivating strain, and inducting method and cultivating method therefor

ActiveCN106544279AMeet the requirements of factory cultivationAchieve commercial productionFungiMagnesium fertilisersPhacusHypha
The invention discloses a morchella esculenta cultivating strain and an inducting method and a cultivating method therefor. An inducting culture medium of a morchella esculenta cultivating strain mother strain is used to induce morchella esculenta; a cultivating culture medium of a morchella esculenta cultivating strain is used to cultivate the morchella esculenta; the inducing method comprises the steps of taking out a preserved test tube strain; inoculating the test tube strain to an inducting culture medium flat plate; culturing in a culture box at a temperature of 18-22 DEG C; after hyphae grow on the whole plate, taking the hyphae as inoculating hyphae to be placed in the cultivating culture medium for use; and the cultivating method comprises the steps of sterilizing, inoculating and performing management on two stages of a mycelium growth period and a sporocarp growth period. The invention, through long-term test, searching and discussion, provides a morchella esculenta artificial cultivating technological method which has breakthrough at home and abroad; the morchella esculenta cultivating strain is an optimized and induced morchella esculenta strain; an integrated technology capable of maintaining stable production and continuous production is achieved; and commercial production is realized.
Owner:秦小波

Method for testing critical freeze damage temperature of plant, and system thereof

The invention discloses a method for testing the critical freeze damage temperature of a plant, and a system thereof, and belongs to the agricultural meteorological disaster monitoring and control field. The method comprises the following steps: putting a plant to be tested in a low-temperature constant-temperature incubator, stimulating natural frost conditions by using the low-temperature constant-temperature incubator, a speed adjustable fan and a humidifier to make the plant to be tested frozen, allowing one end of a working electrode to contact with the plant to be tested, connecting a high precision LCR tester by the other end of the working electrode, applying an AC excitation voltage signal to the working electrode, acquiring the electric signal of the plant by the working electrode, transmitting the electric signal to the high precision LCR tester, converting the electric signal into a digital signal, displaying, storing and processing in a computer for data analysis, and analyzing the change characteristics of the electric signal of the plant in order to obtain the freeze damage temperature of plant. The method and the system can be used for accurately measuring the critical freeze damage temperature of the plant, can be used in airflow disturbance frost prevention control, and provides important control parameters for the airflow disturbance frost prevention control.
Owner:JIANGSU UNIV

Separation, purification and identification methods of human amnion mesenchymal stem cells

The invention discloses separation, purification and identification methods of human amnion mesenchymal stem cells. The separation method of hAMSCs (human amnion mesenchymal stem cells) comprises the following steps: fragmentating human amnion; and carrying out two-step rotating digestion with trypsin of EDTA (ethylene diamine tetra-acetic acid) and collagenase of DNaseI, filtering with a steel mesh and collecting cell filtrate namely separated original hAMSCs. The purification method of hAMSCs comprises the following steps: incubating original hAMSCs with an LG (low glucose)-DMEM (dulbecco modified eagle medium) culture medium in a CO2 incubator; removing amnion epithelial cells which do not perform complete adherence growth under an inverted microscope; replacing a new culture medium on the third day; digesting with a trypsin-EDTA solution after cell converge degree reaches 80-90%; and collecting cells so as to obtain high-purity hAMSCs. The identification method of hAMSCs comprises the following steps: identifying hAMSCs and the amnion epithelial cells by adopting immunocytochemical staining vimentin and CK19; and detecting expressions of CD29, CD44, CD166, CD34 and CD45 by adopting a flow cytometry. The separation and purification methods disclosed by the invention have the advantages of high yield, high activity and high purity of hAMSCs; and the identification method is simple, convenient and precise.
Owner:AFFILIATED HOSPITAL OF ZUNYI MEDICAL COLLEGE

Incubator's canopy with sensor dependent variably transparent walls and methods for dimming lights thereof

InactiveUS20150073204A1Reduce light transmittanceAdversely affecting rest-activity patternBaby-incubatorsStatic indicating devicesPower flowEngineering
An incubator with a canopy that selectively controls an amount of light that is transmitted into it. The incubator includes: at least one sensor configured to detect light transmitted into the incubator's capacity and to further convert the transmitted light into electrical current. The incubator also includes a canopy characterized by at least one portion, the portion comprises one or more smart incubator's glass (SIG); where the SIG is adapted to at least partially reduce or increase the light transparency via the portion in accordance with the sensor's electrical current.
Owner:ASPECT IMAGING

Hospital Equipment and Incubator

InactiveUS20120215054A1No loss of irradianceConsiderable loss in valueBaby-incubatorsAmbulance serviceEngineeringAnti-fog
The present invention refers to hospital equipment, comprising at least an incubator of the closed kind having at least a side wall and at least a lid or dome, thereby defining a cabin inside of which there is provided at least a bed to receive at least a patient, at least one of the walls and / or dome being transparent, operatively associable to, at least, phototherapy equipment, and additionally comprising a film with anti-fog properties associated to at least one of the side walls and / or the dome.
Owner:FANEM

HUVEC (human umbilical vein endothelial cell) separation, culture and subculture method

The invention relates to an HUVEC (human umbilical vein endothelial cell) separation, culture and subculture method belonging to the technical field of biology. The method comprises the following steps: (1) separation of HUVEC: taking a fresh umbilical cord, cleaning with a sterile PBS (phosphate buffer solution) containing penicillin-streptomycin, adding collagenase to perform water bath digestion at 37 DEG C, centrifuging the digestion solution, then adding an M199 culture medium, transferring into a gelatin-coated culture bottle, and culturing in a CO2 incubator at 37 DEG C; (2) culture of HUVEC: after the HUVEC is cultured at 37 DEG C for 24 hours, pouring out the culture medium, cleaning with the PBS, adding a fresh M199 culture medium, and afterwards, changing the culture medium once every two days, wherein the HUVEC can be subcultured generally after being cultured for 5-7 days; and (3) subculture of HUVEC: pouring out the culture medium, cleaning with the PBS, adding the digestion solution to digest the cell, adding a DMEM (dulbecco's modified eagle medium) containing serum to terminate the reaction once the cell is rounded, centrifuging the digested cell, and adding a fresh culture medium, wherein the HUVEC subcultured for 2-3 generations is used for experiments. The separated HUVEC is economical and practical, is simple and easy to use, and is beneficial to obtaining required in-vitro experimental model cells.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES

Rapid cultivating method of sand moss crust

The invention discloses a rapid cultivating method of sand moss crust, Including the steps that a whole piece of the moss crust in a sand natural environment of a dry area is collected, a silver leaf bryum is used as a dominant species, the impurities of sand and dead leaves are sieved to be removed after soaking; the moss crust is crushed after air drying in a shade place or oven drying for 48 hours under 30 degrees centigrade; the underlying sand of the moss crust is collected as substrate and is filled in a cultivating container with the diameter being 18cm, the height being 2cm and the thickness being 1.5cm, the moss pieces are evenly spread on the substrate surface inside the cultivating container, water is added until the water content is 13.9%; the moss pieces are put into a PGX multi-stage serial intelligent illuminating incubator to constantly cultivate for 40 days, and the moss crust can be formed. The cultivating condition requirement of the moss crust is relatively simple, the technology is convenient, the cost is low, and the method can be well popularized in production application.
Owner:NORTHWEST A & F UNIV

Automated sample analyzer and cuvette

An automated sample analyzer (10) has a sample store (20) and a sample dispenser (90) which is controlled by a controller (170). Samples of a substance to be analyzed are dispensed into a cuvette (60), along with a reagent from a reagent storage compartment (40), after which incubation occurs in an incubator (70). The incubated analyte is then passed to a sample analysis module (140). This has a photometric analysis arrangement (150) and a twin photon excitation (TPX) analysis arrangement (160). The incubated analyte is analyzed using one or other of the arrangements (150, 160). By parallel processing of samples in the analysers (150), (160), a rapid throughput of samples is possible but with the possibility of both photometric and TPX analysis. The cuvette (60) is of a tapered design which is particularly suitable for samples to be analysed by either arrangement (150, 160).
Owner:THERMO ELECTRONICS OY

Riemerella anatipestifer indirect coagulation antibody detection kit as well as application thereof

The invention discloses a riemerella anatipestifer indirect coagulation antibody detection kit and application thereof. The kit comprises a riemerella anatipestifer indirect coagulation antigen, standard positive serum, standard negative serum and a diluent. The riemerella anatipestifer indirect coagulation antibody detection kit and the application thereof have the beneficial effects that the kit is simple and convenient to operate, can be widely popularized and used in related grassroots veterinarian departments and farms; the kit only needs a few simple consumable materials and incubators when being used detecting samples without any other device, and can be used even in duck farms with relatively simple and rough conditions; the result can be determined intuitively, and can be observed by eyes; professional knowledge is not required for operators, special training is not required, and the detection can be performed only referring to a specification; the kit is low in cost, is economical and practical, and can be accepted by most of users.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI

Method for detaching cultured cells, cell detachment device used in said method for detaching cultured cells, and incubator

Disclosed is a method for detaching cultured cells that can selectively detach cultured adhered cells. The scaffold to which the cells adhere is configured from at least a cell adhesion factor containing carbon nanotubes, and by means of radiating laser light in a spot shape on the scaffold in a region where cells are adhered, a shock wave is generated by the heat arising by means of the photothermal conversion of the carbon nanotubes, and by means of this shock wave, the cells are caused to be in a non-adhered state.
Owner:JAPAN SCI & TECH CORP

Guinea fowl ecologically breeding method

InactiveCN103766276AFull muscle trainingReasonable ratioAnimal feeding stuffBiotechnologyGrazing
The invention discloses a guinea fowl ecologically breeding method. The method comprises the following steps of a, egg selection: keeping hatching eggs under the conditions of a temperature of 16-19 DEG C and a relative humidity of 60-70% for less than or equal to 10 days, wherein the weight of the eggs is 45-50 grams; b, incubation: incubating the eggs in an incubator at a temperature of 38.5 DEG C and a humidity of 56% in the first 23 days, and then hatching the eggs in a hatching machine at a temperature of 37.2 DEG C and a humidity of 70%; c, chick breeding; d, ground free-ranging of commercial guinea fowl. The guinea fowl ecologically breeding method achieves combination of free-ranging and cage-rearing; the bred guinea fowl is fed in a grazing and artificial feedstuff supplement integrated mode and accordingly obtains sufficient muscle exercising during the free-ranging process; artificially-supplemented feedstuff does not contain antibiotics and is reasonable in proportioning; compared with feedstuff-fed chickens ion the market, the guinea fowl bred through the method is richer in meat nutrients, higher in quality, delicious in flavor, capable of meeting the requirements of green food and applicable to wide popularization and application.
Owner:陈丹

Strawberry virus elimination method

InactiveCN1799341AGuaranteed detoxification efficiencyPlant phenotype modificationHorticulture methodsBudCell budding
The invention discloses a method for strawberry virus-removing, comprising the following steps: 1) putting the potted strawberry with virus into the bio-chemical incubator, culturing in the lethal temperature for virus for 2-3 days; 2) stripping the stem tip nascent tissue of smaller than 0.5mm, inoculating which in the culture medium with concentration of MS half-reduced for its growing; 3) transferring the stem tip nascent tissue when its sprout grows to 0.5-1.5cm in height to induced enrichment culture medium for growing; transferring it to biochemical incubator when generates adventitious bud, culturing under the lethal temperature for 30 minutes; 4) putting the sprout mentioned above into tissue culture room for culturing under 28 Deg C for 30 minutes; 5) repeating the steps 2) to 4) for 1-2 times. The method in this invention does not strip large quantity of stem tip and the virus-removing rate is as high as 100%.
Owner:ZHEJIANG UNIV
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