3087 results about "Incubator" patented technology

An incubator can include a body, a bed supported by the body, and a canopy defining an interior chamber that includes the bed. The incubator can include any combination of various features configured to reduce sound energy inside the incubator. The incubator can include a passive radiator, a Helmholtz resonator, a meta material absorber, an active noise cancelation system, a vented door latch assembly, an acoustically permeable seal, a pressure relief valve, a lift mechanism that uses a belt drive, double paned canopy walls, a bin with insulating compliant elements, a manual pressure release valve, a pressure differential notification system, non-parallel or curved canopy walls, a mattress with reticulated foam, and / or an acoustic filter.

The invention relates to the technical field of biochemical culture, in particular to a biochemical incubator with multiple bins and a method thereof.The biochemical incubator comprises a case, a cavity is formed in the middle of the case, the cavity is divided into a plurality of culture bins by a plurality of partition plates, treatment cavities are formed in the two sides of the cavity, protective shells are connected to the outer sides of the treatment cavities, and storage assemblies are arranged in the culture bins; the storage assembly comprises a movable bottom plate and three groups of longitudinal partition plates; the longitudinal partition plate divides the movable bottom plate into two storage cavities, storage tables are arranged in the storage cavities, and the movable bottom plate is driven by the pushing assembly to move left and right and used for alternately feeding the two storage tables into the culture bin; two groups of sealing covers are arranged outside the storage table, and are driven to be opened or closed by a turnover assembly; according to the incubator, pollution-free conveying of materials is achieved, and meanwhile the stability of environmental parameters of all the bins is maintained; the culture method ensures that different batches of samples are cultured in an independent environment, and cross contamination between microorganism or cell samples is avoided.

The invention relates to a culture method for improving duckweed protein, which comprises the following steps: purifying duckweed in a Hoagland working solution to obtain purified duckweed; mixing the biogas slurry with the Hoagland working solution to prepare a duckweed culture solution; wherein the concentration of the biogas slurry is 1.5%-5%, and the concentration of the Hoagland working solution is 95%-98.5%; after the purified duckweed is put into the duckweed culture solution, the duckweed culture solution is put into an incubator with preset culture conditions to be cultured, and the cultured duckweed is obtained. By adding the biogas slurry into the Hoagland working solution, the protein content of the cultured duckweed can be remarkably increased, the biogas slurry can be efficiently utilized, water resources are saved, an ideal way is provided for wastewater recycling, meanwhile, nitrogen and phosphorus in the biogas slurry can be utilized for promoting synthesis of duckweed protein, the protein synthesis efficiency is improved, and the yield of duckweed is increased. And a good basis is provided for feed research of duckweed.

The invention provides a control method and system for temperature return of a constant-temperature incubator. Wherein the real-time temperature in the constant-temperature incubator is monitored, external environment data is combined, the real-time temperature and a preset threshold value are compared and analyzed, and a temperature return starting signal is generated; based on the temperature return starting signal, a control algorithm based on self-adaptive dynamic programming is adopted, a temperature gradient equalization technology is applied, and a heating control instruction is generated; based on the heating control instruction, constructing a three-dimensional temperature distribution model by using a temperature field reconstruction algorithm, predicting a temperature change trend through a recurrent neural network, and generating temperature prediction information; and on the basis of the temperature prediction information, after a set temperature is reached, fine temperature control is automatically switched to, environmental influence analysis is carried out, measures are taken to offset adverse factors, and a temperature control result is generated. According to the control method and system for temperature return of the constant-temperature incubator, the temperature control precision and the response speed are remarkably improved, long-term stability is ensured, and a reliable and efficient environment is provided for biological experiments.

The preparation method comprises the following steps: mixing saccharomycetes, lactic acid bacteria and bacillus subtilis according to a ratio of 1: 1: 1 to obtain mixed bacteria; 0.25 g of mixed bacteria is taken and dissolved in 5 mL of sterile water for standby application; slicing and crushing the dried rhizoma polygonati, and sieving with a 10-mesh sieve to obtain rhizoma polygonati powder; the preparation method comprises the following steps: mixing water and rhizoma polygonati powder according to a liquid-material ratio of 10mL: 1g, sterilizing at 121 DEG C for 30min, cooling, adding mixed bacteria according to an inoculum size of 3% of the mass of rhizoma polygonati, culturing in a constant-temperature shaking table incubator at 120r. Min <-1 > for 72h at the culture temperature of 035 DEG C, sterilizing at 100 DEG C for 30min after fermentation, cooling and centrifuging to obtain rhizoma polygonati fermentation liquor, and storing at 4 DEG C. The prepared polygonatum sibiricum fermentation liquor is high in active ingredient content, has good cell safety, anti-oxidation, anti-inflammatory and whitening effects, and has the potential of being used for developing functional raw materials of traditional Chinese medicine cosmetics.

The invention discloses stem cell culture equipment, and relates to the technical field of cell culture equipment, the stem cell culture equipment comprises a culture box, a rotating disc and a culture dish, horizontal plates are arranged in the middle of the culture box at equal intervals, and the middle of each horizontal plate is connected with the rotating disc through a bearing. The stem cell culture equipment is provided with a switching mechanism and an anti-deviation mechanism, a limiting ring and a circular track are separated, a conical block drives a tray to push culture dishes to be rapidly taken and placed, a rotating disc rotating slowly facilitates personnel to take or place the culture dishes one by one at the position close to an opening of a culture box, the limiting ring and the circular track are in matched butt joint, and the operation is convenient. The extrusion part elastically extrudes the culture dish on the tray when reversely moving and overturning, and meanwhile, the traction balance wheel is propped against the upper edge of a sealing end cover of the culture dish, so that the culture equipment can prevent the culture dish which is rotationally placed from shaking and shifting, and can be used for lifting and ejecting all the culture dishes at one time when the culture dish is taken out; and the operation of manually placing the culture dishes can also be reduced.

The present invention relates to the field of Chinese medicine cultivation, and in particular to a cultivation device for Chinese medicine planting with automatic temperature control, including a cultivation box, a placement plate, a cultivation frame and an electric push rod; the front and rear sides of the cultivation box are both provided with box doors; the front and rear sides of the cultivation box are respectively connected with a plurality of placement plates; each placement plate is respectively fixed with a plurality of cultivation frames; the front and rear sides of the cultivation box are respectively fixed with a plurality of electric push rods, and each two corresponding telescopic parts of the electric push rods are respectively fixed with a placement plate. The separation plate is rotated to scrape the inner wall of the cultivation frame, and the soil adhered to the inner wall of the cultivation frame is scraped off and separated, so as to facilitate subsequent transplantation, and avoid part of the soil remaining and adhering to the inner wall of the cultivation container, which cannot be fully removed, resulting in the root system of the seedling being pulled by the soil when being removed and damaged, thereby affecting the survival rate of subsequent transplantation.

The invention belongs to the field of agricultural engineering, and particularly relates to an intelligent edible mushroom incubator based on an artificial intelligence internet of things. Comprising an edible fungus culture box body, and an edible fungus growth frame module, a spray atomization module, a carbon dioxide module, a fresh air circulation module, a visual detection module, a growth light module, a temperature sensing module and an intelligent disinfection module which are arranged on the edible fungus culture box body in an assembling manner, the upper surface, the lower surface, the left surface, the right surface and the rear surface of the edible mushroom incubator body are provided with heat preservation interlayers, and the front side surface is of a double-open cold storage door structure. By means of the Internet of Things technology, the artificial intelligence technology and the like, real-time monitoring of the growth environment of the edible mushrooms and accurate mastering of the growth condition of the edible mushrooms are achieved, so that the production efficiency and yield of the edible mushrooms are improved, and meanwhile the production cost and risk are reduced.

The invention relates to the technical field of microbial culture, and provides a culture device for agricultural microbial agent production and a use method, the culture device comprises a culture box, and the top of the culture box is hermetically and detachably provided with a box cover. According to the device, the air pump is controlled by the controller to be started, so that the air pressure in the closed space is increased, the air passes through the non-woven fabric and the polymer microporous membrane, more tiny bubbles can be generated under the action of the polymer microporous membrane, a nutrient solution flows and is mixed, and the stirring effect on the nutrient solution is achieved; a nutrient solution in the incubator flows into the culture dish through the liquid discharge pipe, so that the stirring of the nutrient solution and the injection of the nutrient solution into the culture dish to be mixed with a microbial inoculum can be completed without using more structural components, the operation is simple, and the condition of pollution caused by improper cleaning is avoided; therefore, the subsequent microbial agent culture effect is improved.

The invention relates to the technical field of laboratory equipment, in particular to an incubator heating and cooling temperature detection system and method.The incubator heating and cooling temperature detection system comprises a temperature detection module, a temperature setting module, a temperature calculation module and a temperature control module; threshold data of the test box can be set through the temperature setting module, the temperature calculation module calculates the temperature data based on the threshold data to obtain calculation data, and the temperature control module controls the temperature of the test box based on the calculation data. According to the method, the temperature of the test box can be effectively detected and controlled through the arrangement of the temperature detection module, the temperature setting module, the temperature calculation module and the temperature control module, so that the problem that the temperature of an existing test box cannot be pre-adjusted is solved.

The invention discloses a portable digital constant-temperature incubator which comprises an incubator body shell, an upper cover and an inner container. A heat preservation module is filled between the box body shell and the inner container; a heating module is arranged on the outer side of the inner container, the incubator is provided with a temperature control module, and the temperature control module comprises a controller, a circuit board, a temperature sensor and a temperature displayer. The temperature sensor is installed on the outer wall of the inner container. The temperature control module realizes temperature monitoring, feedback and temperature control; a culture tray and a detection bag bracket are fixed in the inner container; the temperature control module is electrically connected with a power supply module, and the power supply module provides electric energy for the portable digital constant-temperature incubator. Therefore, the invention provides the portable digital constant-temperature incubator which is light, good in temperature control and capable of continuously working in the field.

The tomato grafted seedling raising device comprises a culture box, a first motor is fixedly installed on the side wall of the culture box, the output end of the first motor is connected with a first driving gear, a sliding rail is fixedly installed on the inner side wall of the culture box, and a first culture frame is distributed on one side of the sliding rail; a connecting strip is fixedly installed on the side wall of the first culture frame, a sliding strip is fixedly installed on the side, away from the first culture frame, of the connecting strip, and a rack is fixedly installed at the bottom of the connecting strip; through the arrangement of a first motor, a first driving gear, a rack, culture frames and other structures, the opposite movement of the two culture frames can be conveniently realized subsequently, so that the tomato seedlings cultured in the culture frames can be conveniently and better subjected to photosynthesis subsequently; the two parallel fixed culture frames are prevented from influencing the normal photosynthesis of the subsequent tomato grafted seedlings, and the culture effect of the tomato grafted seedlings is improved.

The invention discloses a gas recognition and concentration detection method and system based on machine learning, and relates to the technical field of gas concentration detection.The method comprises the following steps that relevant data of a box body is obtained based on a transparent incubator, light intensity loss simulation is conducted through an open-circuit type laser gas detector, and light intensity loss simulation data is obtained; based on a multi-perceptron model, constructing a light intensity loss influence model by using the light intensity loss simulation data; constructing a gas detection light path based on an open-circuit laser gas detector, and dynamically collecting the light intensity of laser passing through the transparent incubator to obtain related gas detection data; comprehensive gas concentration detection analysis is carried out, and gas detection management is carried out on the interiors of the transparent culture boxes; the device and the method are used for solving the problem that when the gas concentration in the transparent incubator is detected by an existing gas concentration detection technology, the gas concentration in the transparent incubator cannot be accurately detected in real time under the condition that the internal environment of the incubator is basically not influenced.

The utility model discloses an edible fungus strain constant temperature incubator, which relates to the technical field of incubators, and comprises an incubator body, a limiting block, a thermal insulation layer, an auxiliary frame, a heating device, a fixed plate, a partition plate and a rotating block, the rotating block is positioned in a sliding block, side plates are arranged on two sides of the sliding block, and a top cover is connected above the incubator body. A ventilation opening is further formed in the upper portion of the top cover, a movable block is further arranged in the limiting block, an ultraviolet lamp is connected to the front side of the movable block, adsorption magnets are connected to the two sides of the movable block, and an elastic belt is arranged above the movable block. The multiple sliding blocks and the rotating blocks are arranged in the cultivation box body, the multiple fixing plates can be taken out by taking out the sliding blocks when the fixing plates need to be taken out, strains above the fixing plates are prevented from being damaged, meanwhile, the multiple rotating blocks can change the height of the fixing plates to adapt to different types of strains, and the practicability is high. And the culture work of the strain is ensured to be carried out.

The invention relates to the technical field of cell culture, and particularly provides an adjusting system and method for a cell incubator, and the system comprises a clean gas supply assembly which comprises a first gas driving assembly and a filtering assembly; a first temperature acquisition assembly; a second temperature acquisition assembly; a first humidity acquisition assembly; the controller is used for controlling the first gas driving assembly to drive external air to flow into the cell culture box when the in-box temperature information is smaller than a preset temperature lower limit value, the environment temperature information is larger than the in-box temperature information and the environment humidity information is smaller than a preset humidity upper limit value; the controller is also used for controlling the first gas driving assembly to drive external air to flow into the cell culture box when the in-box temperature information is greater than a preset temperature upper limit value, the environment temperature information is smaller than the in-box temperature information and the environment humidity information is smaller than a preset humidity upper limit value; according to the system, the energy consumption and the temperature regulation cost of the cell culture box are effectively reduced.

The invention discloses an intelligent control method and system for a constant-temperature incubator based on deep learning. The method comprises the following steps: acquiring an equipment state vector in real time; analyzing the equipment state vector by using an improved model, and predicting an environmental parameter demand trend in a culture process to obtain a prediction result; modeling a control strategy into an MDP Markov decision process through a DRL deep reinforcement learning framework according to the prediction result, and dynamically optimizing power distribution of a heater, a refrigerator, a humidifier and a fan by taking minimization of energy consumption and temperature fluctuation as objective functions to obtain an initial control strategy; and adjusting the power of the actuator according to the initial control strategy by using a fuzzy PID algorithm, monitoring sensor data in real time through an isolated forest model, and adjusting the initial control strategy when abnormality is detected to obtain a target control strategy. The environment demand change is pre-judged in advance, and the hysteresis problem of traditional control is solved.

The invention relates to the technical field of stable isotope tracing, in particular to a simple 13CO2 pulse marking device with adjustable volume and height and a method thereof.The simple 13CO2 pulse marking device comprises an incubator, a preparation structure is arranged on one side of the incubator, a pulse marking structure is arranged above the incubator, and a detection structure is arranged on the side adjacent to the preparation structure; a circulation structure is arranged on the side, away from the preparation structure, of the detection structure, a rising structure is arranged at the bottom of the incubator, a plant culture base is arranged above the rising structure, a volume regulation and control structure is arranged above the plant culture base, and an exhaust structure is arranged on the side, away from the detection structure, of the incubator; through organic integration of all the structures, the whole process from plant culture to pulse marking can be achieved in the same device, all the parts are matched with one another, the convenience and efficiency of experimental operation are greatly improved, errors and inconvenience caused by device conversion are reduced, and the experimental efficiency is improved. And an integrated solution is provided for accurately researching plants in different growth stages and related physiological processes.

The present invention relates to the technical field of biopharmaceutical apparatuses, in particular to a sterile culture method and a sterile culture system. The sterile culture system comprises a cell preparation device, an incubator, and a cell culture device; the cell preparation device comprises a preparation chamber, wherein a first sealing valve is arranged on a side wall of the preparation chamber; the incubator has a culture cavity, wherein a second sealing valve is arranged on a side wall of the culture cavity, and the second sealing valve is configured to work with the first sealing valve such that the incubator is hermetically docked with or separated from the cell preparation device; and the cell culture device is used for accommodating the incubator, supplying power and a gas to the incubator, and controlling a cell growth environment in the incubator. Because cells grow in a sterile environment all the time and the pollution risk is relatively low, no frequent sterilization is required. Normal growth of cells is avoided from being affected by frequent sterilization, and the culture time is saved. Therefore, the cell preparation and culture efficiency is relatively high.

The invention discloses laboratory centralized full-automatic analysis five-day biochemical oxygen demand monitoring equipment, which relates to the technical field of five-day biochemical oxygen demand monitoring equipment, and comprises a laboratory centralized full-automatic analysis system, a sample temporary storage incubator, an incubator, a sample transport vehicle and a pretreatment system, the pretreatment system is arranged at the side part of the laboratory centralized full-automatic analysis system. According to the laboratory centralized full-automatic analysis five-day biochemical oxygen demand monitoring equipment, the sample temporary storage constant temperature box, the culture box, the sample transport vehicle, the pretreatment system and other structures are arranged, so that the defect of a laboratory centralized full-automatic analysis system in the aspect of five-day biochemical oxygen demand monitoring is overcome, and the overall automation level of a laboratory is improved; tedious consumable preparation of a laboratory is simplified, operation of experimenters can be greatly simplified, personal errors in the experiment process are reduced, and result accuracy is guaranteed.

The invention relates to the technical field of medical cell culture, and discloses a medical experiment cell culture device which comprises a culture box, an electric push rod is fixedly connected to the lower surface of the culture box, a supporting plate is fixedly connected to the output end of the electric push rod, and a first connecting column is fixedly connected to the outer wall of the supporting plate; a first partition plate is fixedly connected to the inner wall of the incubator, a sliding groove is formed in the first partition plate, the outer wall of the first connecting column is slidably connected to the inner wall of the sliding groove, and a movable frame is rotatably connected to the outer wall of the first connecting column. The electric push rod is started to drive the supporting plate to move up and down, and then the connecting column drives the moving frame to move synchronously. The movable frame drives the transmission plate to move upwards, the frame plates at the two ends are pulled or pushed to move relatively through the fixing columns, then the closing plate is driven to be opened and closed, meanwhile, the placing frame can be moved into a test box or stored into a culture box, operation convenience and efficiency are improved, and the risk that the culture environment is polluted by manual taking is avoided.

The invention relates to the technical field of periphyton incubators, and discloses a device for culturing periphyton, which comprises an incubator body, a control operation panel connected to the top of the incubator body and a culture container connected in the incubator body. Through mutual cooperation of an arc-shaped box, a chain, a chain wheel and a lamp shaft seat, the visible light simulation illumination lamp with the wavelength of 400-700 nm can move and is similar to rising of the sun from low to high and then gradually reduced, so that the illumination position is dynamically changed, a more natural growth environment is simulated, the illumination intensity and the duration time are synchronously controlled, and the illumination effect is improved. Therefore, the proper growth temperature in the incubator is maintained, the change rule of the illumination intensity and the illumination angle in one day is truly represented, artificial stable growth conditions closer to the natural environment are provided for periphyton, and efficient growth and metabolism of periphyton at the proper temperature are promoted.

The invention discloses a method for detecting tumor killing activity of NK (Natural Killer) cells. The method for detecting the tumor killing activity of the NK cells comprises the following steps: recovering and culturing K562 cells; preparing a target cell K562; preparing effector cells NK; the K562 cell suspension and the NK cell suspension are mixed according to the effect-target ratio to serve as an experimental group, the K562 cell suspension serves as a control group, after the experimental group and the control group are incubated in an incubator for a period of time, the cells are transferred into a centrifugal tube and cleaned with PBS, then PI dye liquor is added for incubation, and the cell suspension is obtained. Detecting the apoptosis rate of the K562 cells in the experimental group and the control group by adopting a flow cytometer, and calculating the tumor killing rate of the NK cells; the killing activity of the NK cells to the K562 tumor cells is evaluated through a CFSE / PI labeling method, operation is relatively simple, consumed time is short, sensitivity is high, repeatability is good, and the method has the advantages of being safe, efficient, economical, stable and the like and has good application value.

The utility model discloses automatic calibration equipment, and relates to the technical field of calibration equipment. The calibration tool is used for installing a product to be calibrated, the transfer trolleys are used for carrying the calibration tool, the incubator is provided with a three-axis movement system and an intelligent control system, the manipulator is used for grabbing the calibration tool on the transfer trolleys into the incubator, and a sliding device for driving the manipulator to move is arranged at the lower end of the manipulator. The device has the advantages of being high in automation degree, accuracy and reliability.

A water culture device for peony is provided. The water culture device for peony includes an incubator. The incubator is internally provided with a sliding rack at a middle position. A servo motor is arranged on a right side of the incubator. Second rotating shaft levers are arranged on left and right sides of the incubator. Since the locating clips on the regulating levers and the planting baskets on the sliding rack are used cooperatively, the peony plants can be blossomed and positioned. Moreover, a downward putting action of the planting basket and a location clamping action of the locating clip are combined together and performed in one time through mechanical transmission, so that the trouble of stepwise operation is omitted, and the culture efficiency of peony plants is effectively promoted.

The utility model provides a livestock cub incubator which comprises a fan-shaped incubator body, a fixing sleeve, an excrement scraper and a liquid passing hose, two heat preservation lamps are installed at the upper end of the interior of the fan-shaped incubator body, and a positive and negative rotation motor is installed on the left front side of the upper end of the fan-shaped incubator body. A rotating shaft is longitudinally installed on the left front side of the interior of the fan-shaped heat preservation box body through a bearing, the lower end of the exterior of the rotating shaft is fixedly sleeved with a fixing sleeve, a swing plate is longitudinally fixed to the rear end of the fixing sleeve, a plurality of rubber push blocks are installed at the right end of the swing plate, and a lateral groove is formed in the left end of the swing plate. In order to solve the problem that when excrement of a cub is cleaned regularly, a livestock worker needs to move the cub out of a heat preservation box firstly and then manually clean the excrement of the cub, which is relatively inconvenient, the utility model designs a novel swing type scraper structure, and the excrement of the cub can be automatically scraped out; the cubs can be pushed out of the device in advance in the swinging process to be disinfected.

The utility model discloses a culture fishpond tail water treatment system, which comprises an algae incubator, an algae liquid putting device and a bacteria-algae symbiotic bed, the algae liquid putting device is used for putting algae liquid in the algae incubator onto the bacteria-algae symbiotic bed, and the bacteria-algae symbiotic bed is positioned in the culture fishpond tail water and is used for purifying the culture fishpond tail water. The system integrates the algae liquid circulating device and the algae liquid adding device, the automation degree is high, and compared with a traditional treatment technology, the treatment technology is easy to operate, high in efficiency, low in cost, environmentally friendly, low in carbon and free of secondary pollution, and is an economical and effective mode for purifying the tail water of the aquaculture fishpond.

The invention discloses a semiconductor refrigeration incubator dehumidification control method and system, a medium and equipment, and relates to the technical field of dehumidification control. The method comprises the steps that the current temperature and the current humidity of the intelligent incubator are acquired, and the humidity deviation and the humidity to be adjusted are determined based on the current temperature and the current humidity; determining a current dehumidification stage based on the humidity deviation; based on the to-be-adjusted humidity, determining a first adjusting parameter of the upper-layer refrigeration sheet and a second adjusting parameter of the lower-layer refrigeration sheet in the current dehumidification stage; calculating the first power of the upper-layer refrigeration sheet based on the first adjustment parameter, and calculating the second power of the lower-layer refrigeration sheet based on the second adjustment parameter; and controlling the upper-layer refrigeration sheet to operate according to the first power, and controlling the lower-layer refrigeration sheet to operate according to the second power. By implementing the technical scheme provided by the invention, the dehumidification efficiency of the incubator can be improved.

The invention discloses a cell culture box convenient for taking and placing cell culture dishes, and relates to the technical field of cell culture, the cell culture box comprises a box body, the inner wall of the box body is fixedly connected with a plurality of placing plates, and the side walls of the top ends of the plurality of placing plates are provided with a plurality of first grooves. The inclination angle of the second clamping plate is the same as that of the outer wall of the corresponding cover plate, so that the cover plate is screwed above the cell culture dish by utilizing the second clamping plate, the primary installation between the cell culture dish and the cover plate is completed, and then a buckle on the outer side of the cover plate can be pressed into a clamping groove in the outer wall of the cell culture dish through a clamping block; when the cell culture dish needs to be taken out, a buckle is pulled out of a clamping groove in the outer wall of the cell culture dish by utilizing a pulling plate, and then the cover plate is taken down from the cell culture dish by utilizing a second clamping plate, so that the culture efficiency and reliability during cell culture are greatly improved.

A sweet potato leaf in-situ instantaneous transformation method comprises the following steps that sweet potato stem segments are sheared and cut into a matrix or water, and the sweet potato stem segments are placed in a special incubator to be cultured till rooting; transforming a plasmid carrying a target gene into agrobacterium through a standard transformation program to obtain agrobacterium for infection, and adding the agrobacterium for infection into an infection solution to obtain an agrobacterium infection working solution; the method comprises the following steps of: pricking holes around veins of new leaves of a cultivated sweet potato plant, inversely putting the sweet potato plant into a vacuum flask filled with an agrobacterium infection working solution, completely immersing the leaves into the agrobacterium infection working solution, and treating for 40-50 seconds under the vacuum condition of 0.6-0.8 pa; and immediately transferring the infected plant into a turnover box for dark culture, and transferring the plant into a professional incubator for continuous culture after dark culture is finished, thereby obtaining the target gene introduced sweet potato transformed plant. The method can simplify the operation, shorten the time and improve the expression effect, and can be used for researching gene functions.

The utility model discloses an edible fungus cultivation box with a humidifying function, and relates to the technical field of edible fungus cultivation, the cultivation box comprises a box body, a box door is hinged to the front end of the box body, a plurality of sliding grooves are symmetrically formed in the box body at equal intervals, sliding blocks are connected in the sliding grooves in a sliding mode, and a cultivation plate is fixedly connected between every two sliding blocks; a humidifying structure is arranged in the box body, so that edible mushrooms cultivated on each cultivation plate in the box body can obtain equal irrigation water sources, the growth of the edible mushrooms can be ensured, the water resources can be fully utilized, the waste of the water resources is reduced, and the production cost of edible mushroom cultivation is reduced; a heat dissipation structure is arranged at the bottom of the rear end of the box body, fan blades in a mounting base flap the interior of the box body, the purposes of accelerating air flowing and reducing the temperature in the box body are achieved, through forming of through holes in the two sides, normal ventilation is conveniently conducted in the cultivation box, and then normal respiratory metabolism of edible mushrooms is ensured.