281 results about "Incubator" patented technology

This invention discloses a hepatocyte model with silencing or overexpression of LCMT1 and its construction method, comprising: passage and seeding cells in logarithmic growth phase into well plates; diluting plasmid siRNA1 or siRNA2 or an overexpression plasmid to an appropriate concentration, and then mixing it with transfection reagent dilution to obtain a transfection mixture; washing the cells in each well of the plate and adding another culture medium, then gently dripping the transfection mixture into the well plate, shaking well, and culturing in a cell culture incubator for a period of time; replacing the culture medium with complete culture medium without antibiotics to obtain the constructed hepatocyte model with silencing or overexpression of LCMT1. This invention, by constructing a hepatocyte model with silencing or overexpression of LCMT1 in the liver, allows for validation experiments to be conducted to study the development mechanism of liver fibrosis. It is suitable for widespread application in various laboratories and provides an experimental model basis for research on liver fibrosis and drug trials.

1. The name of the design product: egg incubator (16 PRO). 2. The use of the design product: applied to egg incubation. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

1. Name of the product in this design: Incubator (Parrot Incubator). 2. Purpose of this design: For keeping parrots warm. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The utility model relates to the technical field of breeding equipment, concretely to an intelligent gosling incubator, including the casing, controller, waste board, the controller fixed mounting is in the casing, the casing bottom is opened with the chute, the fixed mounting of slide rail is in the chute, the waste board fixed mounting is in the slide rail, be equipped with the drive arrangement for driving the waste board movement on the casing, and the drive arrangement is electrically connected with the controller through the electric wire, and the controller is equipped with the drive program, the heating lamp is still equipped on the inside top wall of casing, and the temperature control program is still equipped in the controller. The utility model through being equipped with the waste board receiving the excrement of young goose, still be equipped with the drive arrangement to the waste board is pushed, and the drive program can be started in time to push the excrement to the outside of casing in time, and the alarm lamp is equipped to prompt the staff to clean the excrement in time, and the waste board is placed with multilayer nylon cloth, and the artificial only needs to take away the one layer nylon cloth with the excrement and can.

The utility model provides a plant soilless culture incubator capable of accurately controlling temperature, which relates to the technical field of plant cultivation equipment and comprises a box body, a revolving door is mounted on one side of the box body, a group of vertical supporting legs are mounted at corners of the bottom of the box body, and a stabilizing plate is arranged on the outer side of the box body. A positioning groove is formed in the inner side of the bottom of the box body, a storage shell is installed in the positioning groove, and the two sides of the inner side of the box body are each provided with a partition plate. The supporting frame is arranged to be matched with a rapid locking and unlocking structure of the locking frame, the cultivation height can be flexibly adjusted according to the plant growth stage, and the requirement for mixed cultivation of multiple types of plants is met; the design of the multi-layer cultivation frame effectively improves the utilization rate of cultivation space, solves the problems that the space of a traditional cultivation box is fixed and plants are crowded after growing up, and also solves the problem that a traditional culture dish is fixed through bolts.

This invention relates to the field of environmental remediation technology, specifically providing a method for the synergistic degradation of chlorinated organic pollutants using anaerobic microorganisms and iron-manganese minerals. The method includes the following steps: S1, after deoxygenation, water containing chlorinated organic pollutants is inoculated into a bacterial culture system, and the reaction system is placed in a constant-temperature shaking incubator for anaerobic reaction; S2, iron-manganese minerals are added to the reaction system, and a secondary reaction is carried out under aerobic conditions, thus completing the degradation of chlorinated organic pollutants. This invention significantly improves the overall purification efficiency of complex pollution systems containing chlorinated organic pollutants through an anaerobic-aerobic coupling and a synergistic biodegradation-chemical oxidation strategy, demonstrating promising application prospects.

This invention provides a method and apparatus for collecting earthworm burrow wall soil. The method includes collecting raw soil, adjusting the particle composition of the raw soil to obtain culture soil. A mesh screen is laid in a culture pot, and the culture soil is placed inside the mesh screen. Earthworms are placed in the culture soil, and the culture pot is placed in an incubator. The environmental parameters in the incubator are adjusted to allow the earthworms to construct burrows within the culture soil. A preset time is checked; if so, the culture soil is removed, and the burrow wall soil is peeled off. The culture soil balances plasticity and permeability to meet the needs of earthworm growth and burrow building during long-term cultivation. By adjusting the temperature, humidity, and light intensity in the incubator, the earthworms can fully construct burrows while maintaining stable burrow wall soil properties. A hard, pointed spoon is used to precisely peel off the burrow wall soil, ensuring that the burrow wall soil sample is free from cross-contamination and structurally intact.

The present invention belongs to the technical field of sensorineural hearing. Provided is the use of SPTBN1 as a target in the treatment of GJB2-related sensorineural hearing loss. The use comprises: the construction of a stably transfected cell line, wherein: HEK293T cells are cultured using a DMEM culture medium supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin in a humidified incubator at 37ºC with 95% air and 5% CO2, and when the cells reach 80%-90% confluence, cell passage is performed; IP-MS analysis, wherein: protein complexes are purified using Protein A / G immunoprecipitation magnetic beads, a portion of the extracted proteins is used as input, and then 2 μg of anti-Cx26 antibody is added to the remaining protein extract, same are gently pipetted and mixed, and incubated on a rotating shaker at 4℃ overnight; immunofluorescence observation of the co-localization of Cx26 and SPTBN1, wherein: a stably transfected cell line expressing WT-Cx26 and Mut-Cx26 is constructed; and co-immunoprecipitation (Co-IP) validation, wherein: a stably transfected cell line expressing WT-Cx26 is constructed. The present invention overcomes the limitations of therapeutic methods, such as the relatively low targeting specificity and short therapeutic time windows associated with full-length protein supplementation via gene therapy, thereby providing new insight into GJB2-related hearing loss, and a new target and a new treatment for same.

The utility model belongs to the test equipment technical field discloses a constant temperature box, including box, cooling mechanism and drive mechanism. The box is equipped with constant temperature cavity, and the lateral wall of box is equipped with the air inlet that links constant temperature cavity and outside. Cooling mechanism includes casing and fan, and the cavity is equipped in casing, and the air inlet and air outlet that link the cavity are equipped on casing, and the fan sets up in the cavity and is located at the air outlet, and the casing is slidably connected in the lateral wall of box that is equipped with air inlet, and the casing has cooling work position and plugging work position, and when the casing is located in cooling work position, the air outlet links the air inlet, and when the casing is located in plugging work position, the lateral wall of casing blocks the air inlet. Drive mechanism sets up on the box and is used for driving the casing to switch between cooling work position and plugging work position. Set up movable cooling mechanism, can guarantee the stability of temperature in constant temperature box, and can carry out the cooling of electronic component in constant temperature box when needing cooling.

The present application relates to a kind of constant temperature shaking incubator for immunodetection kit and its shaking frequency adjusting method, belong to immunological analysis equipment technical field, including box, constant temperature module, shaking platform, drive mechanism and control system, still include: online temperature detection unit, set in the inside of box, for real-time acquisition kit current temperature signal;And optical detection unit, set in the above of shaking platform, for real-time monitoring the turbidity or light transmittance change of reaction liquid in kit, target temperature-time curve and target turbidity-time curve are built in control system, for receiving the feedback signal of online temperature detection unit and optical detection unit.In the present application, it solves the problem that traditional incubation equipment cannot dynamically adjust the shaking frequency according to the reaction process, leading to low reaction efficiency and large batch difference, significantly improves the sensitivity, specificity and result consistency of immunodetection.

The application relates to a temporary culture box and a temporary culture box hoisting system, wherein the temporary culture box is used for temporary culture of fish groups in a fish collecting and transporting process, the temporary culture box comprises a box body, the box body comprises a top and a bottom, the top comprises at least one cross beam and two connecting pieces located at two ends of the cross beam; a photoelectric positioning module is arranged in the middle of the cross beam, the photoelectric positioning module is used for sending box position information of the box body to a crane; the connecting pieces are provided with first connecting lines, a plurality of the first connecting lines converge to the same vertex in a direction away from the bottom, so that a hoisting grab of the crane is automatically lowered to a target position at the vertex according to the box position information of the box body. The application can make the hoisting grab of the crane automatically position the target position needing to be lowered by using the box position information of the photoelectric positioning module, save labor cost, improve fish group transportation efficiency and survival rate.

The utility model relates to the field of seed cultivation box discloses a seed cultivation box of automatic light supplementing, including cultivation box body, the front end of cultivation box body is rotatively connected with the revolving door, the middle part of revolving door is equipped with the observation window, the inside fixedly connected with glass in observation window, the top of observation window is provided with cleaning assembly, the inner wall of cultivation box body left and right two ends all are fixedly connected with fixed slide rail and fixed strip, and fixed strip is located in the front end of fixed slide rail, the fixed strip inside is connected with the sliding block of sliding, the sliding block is connected with the baffle through the dismounting assembly, the front end of baffle is equipped with the moving groove. In the utility model, through the control limit rod of paddle, easily realize the baffle rotation and height adjustment, satisfy the demand of different seed cultivation to the baffle distance, height, simultaneously, when disassembling the baffle, need only to make it separate from the slot by poking the limit strip, can separate the baffle with the sliding block, convenient for staff to disassemble and clean or maintain quickly.

1. The name of the design product: cell culture incubator. 2. The use of the design product: the design product is used to provide a stable constant temperature environment for cell culture. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective drawing.

A method for improving the germination of *Carex lumbricoides* seeds based on nano-zinc oxide initiation belongs to the field of wetland ecological engineering technology. The invention includes the following steps: First, nano-zinc oxide particles with a particle size of 30±10 nm are dispersed in deionized water by ultrasonic oscillation to prepare a suspension of 12.5-200 mg / L; second, collected *Carex lumbricoides* seeds are float-screened, and plump seeds that sink to the bottom are selected and subjected to surface sterilization; subsequently, the sterilized seeds are immersed in the nano-zinc oxide suspension and initiated by agitation under light-proof conditions for 6 or 24 hours, followed by air drying to restore the initial moisture content; finally, the initiated seeds are placed in an artificial climate incubator for germination culture under specific variable temperature and light conditions. This invention utilizes the biocompatibility of nano-zinc oxide and effectively solves the problems of long germination cycles and low germination rates of *Carex lumbricoides* seeds by precisely controlling the initiation concentration and time. This invention has the advantages of simple operation, low cost, and environmental friendliness, and can significantly improve the germination rate and germination percentage, providing high-quality seedlings for the restoration of degraded wetland vegetation.

The utility model relates to the technical field of egg hatching, specifically to an egg hatching device for agricultural breeding, which comprises heating fins, a clamping groove is formed on one side of the heating fins, an electrostatic filter screen is clamped and installed in the clamping groove, and a hatching mechanism is arranged on the outer side of the heating fins through bolts. The utility model realizes the synergic regulation and control of temperature, humidity and oxygen concentration through a closed-loop control system, eliminates temperature fluctuation when oxygen is supplemented in combination with a preheating air inlet module and a circulating air duct, significantly improves the stability of the hatching environment and the survival rate of embryos, maintains the airtightness of the main sealing door when the observation window is independently opened and closed through the driving of a sub-control circuit on a double-layer door structure, realizes pollution-free monitoring and reduces heat energy loss, and meets the requirements of efficient hatching and energy saving.

The utility model discloses a novel mould incubator, including mould incubator, the inner chamber left and right sides of mould incubator from top to bottom all are symmetrical and install the base, the inner chamber outside both sides of base all are symmetrical and are equipped with the clamping hole, the inner chamber of base is inlayed and has the moving seat, the outside central position of moving seat is provided with the ball, the ball is mutually clamped between clamping hole, the upper and lower both ends of moving seat all are installed the gyro wheel, the inner side extension of moving seat is the outer wall of base and is installed the base. The device simple structure, reasonable in design, it adopts the mode of pulling out, when taking and placing the culture dish, can move the tray and operate, not only avoid the space limited, prevent touching the surrounding culture dish, convenient to use, and also facilitate the disassembly of tray, a series of operation such as subsequent maintenance and overhaul, use nimble, strong practicality, satisfy the use demand on the market.

The utility model relates to the field of moisturizing breeding technology, and disclose a kind of special moisturizing breeding box of black-spotted frog tadpole, solve the current special moisturizing breeding box of black-spotted frog tadpole cannot guarantee that every tadpole epidermis is humid, easily affect tadpole growth state Problem, it includes box, the wastewater collection box is connected in the box inside one side, the water guide hole is established in the bottom end of the box inside, the wastewater collection box is located below water guide hole, the fixed hole is established in the box upper end one side, the utility model is set through the setting of moisturizing component, by water pump delivery water flow to spray cover, by spray cover spray small water mist, pull up rotating sleeve upwards, rotate rotating sleeve, under the setting of slider and sliding slot, rotating sleeve rotation does not affect the rotation of support plate, rotating sleeve rotation drives round bar rotation, round bar drives spray cover all-around spray water mist, ensure that every tadpole skin contacts water mist, avoid tadpole epidermis dry, affect growth state.

The application relates to the technical field of incubator control, and discloses a temperature calibration method and device for an incubator and the incubator. The temperature calibration method comprises the following steps: acquiring a real-time temperature detection value in the operation process of the incubator; acquiring a power parameter of a heater of the incubator under the condition that the change of the real-time temperature detection value indicates that the incubator is in a temperature stable state; determining a corresponding calibration correction value according to the power parameter of the heater; determining an actual calibration value according to the calibration correction value and an initial calibration value of the incubator, and calibrating the temperature detection value of the incubator by using the actual calibration value. The calibration accuracy of the temperature detection value of the incubator under different operation conditions can be effectively improved.

The utility model provides a kind of constant-temperature planting box for seed production with temperature regulating effect, belong to agricultural planting technical field, including constant-temperature incubator, the air vent being connected to the top of constant-temperature incubator, the door being hinged to the outside of constant-temperature incubator, and the control panel being adapted to install on the outer surface of constant-temperature incubator;Heat dissipation mechanism, including the drive assembly for sending out the excess heat in constant-temperature incubator.This utility model can drive centrifugal component to produce centrifugal force by drive assembly to produce wind force when the temperature of constant-temperature incubator rises and needs to dissipate heat, automatically drive sealing disc to rotate and open air vent by centrifugal force, and when there is no need to dissipate heat, drive assembly is closed and centrifugal force disappears, centrifugal component resets by its own gravity, drives sealing disc to close air vent, to avoid excessive heat loss or too much external heat from entering, to provide stable and suitable growth temperature environment for seeds.

This invention discloses a fully automated cell culture incubator, comprising an incubator, a conveying mechanism, and a storage rack assembly. The storage rack assembly can store multiple sets of culture plates and is rotatably docked with the conveying mechanism. The conveying mechanism can grasp the culture plates on the storage rack assembly and convey them to the outside of the incubator for docking. The advantages of this invention are that the conveying mechanism can grasp and convey the culture plates on the storage rack assembly to the incubator, and simultaneously convey and dock external culture plates with the storage rack assembly. This device can store and culture batches of organisms through the storage rack assembly, seal the incubator through a sealing door assembly, and filter impurities inside the incubator to ensure a suitable growth environment for the samples.

The utility model relates to biological engineering equipment technical field, concretely relates to a biochemical incubator, including base, is equipped with the box on the base, is fixed in the box incubator, is pasted with the conduction sheet on the incubator, and the semiconductor refrigeration heating module is connected with the conduction sheet, and one side of the box is equipped with air filtration system, and is communicated with the incubator through the circulation pipe, and the ultrasonic humidifier is equipped below the semiconductor refrigeration heating module, and is communicated with the incubator through the pipeline, and the incubator is equipped with the sealed door, and there is the gap between the incubator and the box and forms the heat insulation layer, and the controller is connected with the temperature and humidity sensor group, gas sensor group electric signal, can automatically regulate and control each module according to real -time data.

This utility model discloses a seed growth observation box that can simulate an environment, including a box body with observation windows symmetrically arranged on both sides of the box body surface. Two sealed door panels are installed at one end of the box body surface, and handles are installed on the surface of the two sealed door panels. A base plate is horizontally fixed inside the box body, and a sliding plate is horizontally slidably connected to the top of the base plate. A sliding mechanism for sliding the sliding plate is provided inside the box body. An incubator is rotatably connected to the center of the top of the sliding plate. A rotating mechanism for rotating the incubator is provided inside the box body. A lifting frame is vertically slidably connected inside the box body and is located on the top of the incubator. This utility model, through the cooperation of the incubator and the sliding plate, allows the incubator to be rotated during use, making it easier to observe the simulated seed growth inside the incubator. At the same time, by sliding the sliding plate, the incubator will also slide together, thereby facilitating operation inside the incubator and improving the ease of use of the device.

This invention discloses an incubator, comprising a housing with a power mounting area and a reaction tube incubation chamber inside. A drive motor is installed in the power mounting area, and a magnetic block is eccentrically mounted on the motor shaft of each drive motor. The reaction tube incubation chamber has mounting holes for placing reaction tubes, and a receiving space is formed inside the reaction tubes to hold rotors. The advantages of this invention are: the eccentric arrangement of the drive motor and magnetic block allows the drive motor to rotate the magnetic block, which in turn drives the rotor to rotate and stir. During stirring, the rotor provides tangential forces along the circle of rotation and forces in all directions of normal, subjecting the liquid inside the reaction tube to forces from multiple directions, creating turbulence. This results in more uniform and stable stirring of the liquid inside the reaction tube, improving the accuracy of the detection results.

This invention provides a device and method for real-time monitoring of stem cell exosome therapy based on a cardiomyocyte sensing system, belonging to the field of micro-nano sensing and exosome therapy monitoring technology. The device includes a cell electrophysiological sensing element 1, an electrophysiological signal acquisition module 2, a signal amplification module 3, a signal display module 4, and a cell culture incubator 5. These components are sequentially connected via cables and data lines, forming a complete signal pathway. This device is used to detect the process of embryonic stem cell exosome therapy. Through multi-concentration comparison and continuous monitoring at multiple time points, it realizes the development and utilization of a new method for monitoring embryonic stem cell exosome therapy. The detection process is real-time, non-invasive, high-throughput, and highly sensitive; the detection method is efficient and simple, and has promising applications in clinical diagnosis and treatment.

The utility model discloses a corn breeding frame of quick assembly relates to corn breeding frame technology, and the fixed plate top is equipped with fixed column, and the fixed column inside is equipped with the sliding slot, and the sliding slot inboard slidingly connected with the sliding block, the sliding block outboard is equipped with the rack, and the rack inboard is to the incubator clamping, the device main part inside is equipped with the drive mechanism, and the drive mechanism bottom is connected with the fixed establishment, and the fixed establishment includes the second threaded shaft, the second threaded shaft outboard is equipped with the second threaded bushing, and the second threaded bushing outboard is equipped with the limit support, and the limit support is with the rack fixedly bonded. Through the rack in the rack of multiple groups of incubator arrangement, then through the sliding block and the sliding slot and connect, to make the fixed column support multiple groups of incubator and the rack, and simultaneously start the drive mechanism, make the drive mechanism drive the second threaded shaft rotation, to make the second threaded shaft drive the second threaded bushing and the limit support drop, to make the limit support to the rack bonded fixed.

This utility model discloses an intelligent constant temperature and humidity incubator for edible fungi, belonging to the technical field of edible fungi incubators. It includes a cabinet with elongated air vents on its inner sidewall and a flow equalization mechanism on the outer back of the cabinet. The flow equalization mechanism includes an airflow mixing chamber sealed to the back of the cabinet. A vortex hood is fixed to the side of the airflow mixing chamber that contacts the cabinet, and a centrifugal impeller is rotatably mounted inside the vortex hood via a shaft. By setting an airflow control mechanism on the incubator, the treated airflow is buffered in the airflow mixing chamber. Combined with a flow equalization device consisting of a drive motor, transmission components, vortex hood, and centrifugal impeller, the airflow is further accelerated and distributed, allowing it to enter the shelf layers of the incubator with a wider coverage area and stronger power. This ensures that the airflow flows fully around the edible fungi container, guaranteeing efficient growth of the fungi under constant temperature and humidity conditions.

The utility model belongs to mushroom planting technical field relates to a mushroom planting incubator convenient to spray water, including incubator, the inside of incubator is fixedly connected with two placing seat from top to bottom in proper order, the top of two placing seat all is provided with the culture frame of mushroom, the top of two culture frames all is fixedly provided with fixed shell, and the water inlet assembly is connected on two fixed shell, the bottom of two fixed shell inner chambers all is fixedly connected with the mounting panel, every mounting panel all is provided with a plurality of second through -hole, every mounting panel bottom surface middle part all is rotatably connected with the connecting shaft, the outer surface on the bottom of connecting shaft all is uniformly fixedly connected with a plurality of fan blade, and the fan blade is inclined setting. The utility model has the advantages of: the spray head can spray water to the fan blade, and the impact of water flow on the mushroom can be reduced under the block of the fan blade, and the water will impact the fan blade to rotate, so that the water is evenly sprayed on the mushroom in the culture frame, and the water spraying effect is good.

The automatic analysis device of the present application includes an incubator cover (106) configured to cover a pretreatment area provided on a work surface (22) on a housing, an opening and closing cover (105) configured to be able to open and close freely to cover an opening portion provided on the incubator cover (106) for accessing the inside from the outside of the pretreatment area, and a safety cover (3) configured to cover the work surface (22) and the upper portion of the pretreatment area, in a case where the opening and closing cover (105) is in an open position and the safety cover (3) is in an open position, the opening and closing cover (105) moves to a closed position as the safety cover (3) moves from the open position to the closed position. Thus, the opening and closing cover provided at the opening for accessing the area covered by the cover can be reliably closed.