832 results about "Cell model" patented technology

The present invention relates to a computer-implemented method and system for optimizing geometric configurations within thermal energy transfer device comprising at least one thermal energy exchange channel to enhance performance. The method involves obtaining target design criteria for a physical object and defining a unit cell dimensioned such that the thermal energy transfer channel is formed by a matrix of N1 x N2 identical unit cells. Shape optimization is performed at the unit cell level by iteratively modifying the boundary surfaces of solid structures within a unit cell based on criteria including performance. Optimized design parameters are outputted and transferred to production equipment for creating an optimized thermal energy transfer device. This device comprises a matrix of structures arrayed in at least one dimension, derived from optimized unit cell models.

Techniques are described for using machine learning to extract physics-based cell model parameters from battery charge data provided by the battery module of the battery management system. This data may be processed via machine learning techniques in order to periodically update or adapt a physics-based model that may capture cell electrical dynamics and degradation mechanisms. The techniques may utilize a “digital twin” to adaptively charge the battery while preserving the life of the cells.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine prescription efficacy quantification method and system.The method comprises the steps that chemical component spectrums, pharmacodynamic substance contents and biological activity indexes of traditional Chinese medicines forming a prescription are obtained; constructing a multi-target regulation and control network by combining a traditional Chinese medicine-target interaction database according to the chemical component spectrum, the pharmacodynamic substance content and the biological activity index, identifying a core efficacy pathway cluster by adopting a Louvain algorithm, and calculating a pathway activation intensity value by adopting a pathway target membership weighting model; according to the core efficacy pathway cluster, establishing a mapping relation between the core efficacy pathway cluster and traditional Chinese medicine efficacy terms through ontology and semantic mapping, and according to pathway activation intensity values, generating a prescription efficacy intensity vector through a weighted aggregation model; according to the efficacy intensity vector of the prescription, verifying and correcting the network weight through an in-vitro cell model, and generating the efficacy quantification of the prescription in combination with the clinical syndrome calibration coefficient. Therefore, the problems of single data dimension and insufficient quantification result accuracy in the prior art are solved.

The invention relates to the field of biological imaging, and particularly discloses an in-vitro cell microenvironment dynamic monitoring method for two-photon frequency domain fluorescence lifetime imaging, which comprises the following steps: S1, by using a two-photon frequency domain fluorescence lifetime imaging TP-FD-FLIM technology, losslessly representing microenvironment change through fluorescence lifetime parameters, and monitoring oxidative stress reaction and neutrophil migration behavior in real time, analyzing the effect of the associated compound in combination with an in-vitro cell model; and S2, based on the physical basis of FD-FLIM, establishing a frequency domain fluorescence lifetime signal processing and analysis system under periodic excitation, analyzing the dynamic correlation characteristics of the phase delay phi and the modulation depth M of a fluorescence signal relative to exciting light, and carrying out quantitative backstepping on molecular lifetime parameters. According to the in-vitro cell microenvironment dynamic monitoring method for two-photon frequency domain fluorescence lifetime imaging provided by the invention, neuroinflammatory response can be observed in a lossless and real-time manner on the subcellular scale.

The invention belongs to the field of lattice structure design, and particularly discloses a continuous fiber reinforced composite material strength Poisson's ratio adjustable design method and system.The design method comprises the steps that the Poisson's ratio structure type needed by a continuous fiber reinforced composite material component to be prepared is determined, the Poisson's ratio structure type comprises a zero Poisson's ratio structure, a positive Poisson's ratio structure or a negative Poisson's ratio structure; according to the method, regular hexagonal unit cell models and hourglass-shaped hexagonal unit cell models are constructed, and according to the Poisson's ratio structure type, a plurality of regular hexagonal unit cell models and / or a plurality of hourglass-shaped hexagonal unit cells are utilized to construct a corresponding honeycomb porous structure model, so that the Poisson's ratio adjustable design of the continuous fiber reinforced composite material is realized. By means of the method, standardized design of material structures of high-strength, light-weight, vibration-reducing and energy-absorbing parts needed by various parts in different application fields can be achieved.

The invention discloses a breast adenoma B3 type source cell strain as well as construction and application thereof, the breast adenoma B3 type source cell strain is named as human breast adenoma cell Hthy2301 Homo sapiens and is preserved in China Center for Type Culture Collection on March 14, 2024, and the preservation number is CCTCC NO: C202478. The human thymoma cell line is stable in character, can be passaged in vitro for a long time, and has high proliferation activity. The human thymoma cell line disclosed by the invention can be used as an effective cell model, and a new experimental model can be provided for deeply researching the occurrence, development and transfer mechanisms of thymoma, the drug resistance mechanism, drug screening, drug effect evaluation and the like.

The invention discloses a lubricant adsorption and friction regulation and control method based on molecular dynamics simulation, which comprises the following steps: S1, constructing a lubricant molecule and friction pair metal unit cell model, generating topological structure information based on a minimum structure unit, and constructing a lubrication system model with a sandwich structure; s2, giving force field parameters to the lubrication system model, and performing geometric structure optimization and dynamic relaxation; s3, giving surface charges to the upper and lower friction pairs for targeted adsorption simulation of the lubricant; and S4, applying uniformly distributed contact pressure and constant sliding speed to the upper friction pair to perform friction simulation of the lubrication system. According to the lubricant adsorption and friction regulation and control method based on molecular dynamics simulation, a targeted adsorption strategy of a solid-liquid interface is established, the microcosmic adsorption process of the lubricant is reproduced, controllable regulation of friction characteristics is achieved, the human resource cost in actual experiments is reduced, and manual operation errors are reduced.

The invention discloses a rapid equalizing charging method for a lithium ion battery pack, and belongs to the field of battery charging. The method comprises the following steps: step 1, establishing a first-order enhanced equivalent circuit model, constructing an aging model in combination with an Arrhenius equation, and comprehensively establishing an electric heating aging fusion model; 2, constructing a battery pack model through series connection based on a single battery model of the electrothermal aging fusion model; 3, configuring a deep reinforcement learning environment on the basis of the electrothermal aging coupling model, wherein the deep reinforcement learning environment is used for simulating the real state of interaction between a deep deterministic policy gradient algorithm (DDPG) and the battery pack in deep reinforcement learning; 4, a deep reinforcement learning Actor network, a Critic network and a reward function are constructed, and the target of the reward function is defined as the shortest charging time of the battery pack, SOC (state of charge) balance and minimum aging loss so as to support operation and result evaluation of a DDPG algorithm; and 5, training the model to obtain the optimal fast equalization charging current.

The invention discloses a modeling method and a processing device for a lattice structure gear filled with a three-period minimal curved surface, and the method comprises the following steps: writing an implicit function equation of the three-period minimal curved surface by using Matlab numerical analysis software, and generating an stl format model; using Geomagic reverse modeling software to perform reverse modeling on the three-period minimal curved surface cell elements to produce an stp format model; using Matlab numerical analysis software to generate data points, and importing Proe software to generate a gear model; after operations of mirroring, arraying and the like are carried out on the curved surface cell element model by using SolidWorks, a lattice structure is obtained by using a curved surface thickening command; according to the method, SolidWorks is used for dividing the gear into three parts, namely a gear tooth design area, a hub design area and a spoke design area, the spoke design area intersects with a lattice structure Boolean and then is combined with the gear tooth area Boolean and the hub area Boolean, and therefore the lattice structure gear filled with the three-period minimal curved surface is constructed, and a new thought and method are provided for lightweight design of the gear.

The invention relates to application of cembratrienol in preparation of a medicine for preventing and / or treating psoriasis, and belongs to the technical field of biological medicine. The invention provides an application of cembratrienol in preparation of a medicine for preventing / or treating psoriasis. Researches find that cembratrienol can significantly inhibit secretion of inflammatory factors in a psoriasis-like cell model induced by M5; moreover, research finds that cembratrienol can significantly improve the skin lesion condition of an imiquimod-induced psoriasis mouse model, improve the weight loss degree of the psoriasis mouse model, relieve the pruritus condition of the psoriasis mouse model, improve the skin inflammation of the psoriasis mouse model, repair the skin barrier of the psoriasis mouse model, and improve the curative effect of the psoriasis mouse model. The body immune response of the psoriasis mouse model is reduced. Therefore, cembratrienol has a great application prospect in preparation of the medicine for preventing / treating psoriasis.

The invention relates to the technical field of medicines, in particular to a construction method and application of a photothermal conversion platform for treating osteoarthritis by regulating synovial macrophage polarization. According to the present invention, the M2 membrane-structured black phosphorus selenium carbon body (M2-BPSeC) photo-thermal nano-platform is innovatively provided, the triple functions of bionic targeting delivery, precise thermal control intervention and immune microenvironment remodeling are integrated, and the excellent synergistic treatment effect is represented in the in-vitro cell model and the OA animal model. The technology breaks through the technical bottleneck that traditional treatment is insufficient in targeting and single in intervention dimension, precise functional coupling of the physical thermal effect and immunoregulation is achieved for the first time, and a brand new strategy of targeting-temperature control-immunity integration is provided for osteoarthritis treatment. The efficient and controllable preparation process and the remarkable multi-dimensional treatment effect open up a new path for cartilage regeneration and immune balance regulation and control.

The invention relates to the field of photovoltaic technology, in particular to a photovoltaic cell model parameter extraction method and related products, and the method comprises the steps: building a photovoltaic cell mathematical model, and determining to-be-optimized model parameters in the mathematical model; constructing a fitness function of the photovoltaic cell mathematical model; constructing a parameter optimization model based on a whale optimization algorithm and a differential evolution operator, and taking a fitness function as a target function; executing the parameter optimization model, and obtaining an optimal parameter set corresponding to model parameters to be optimized in the mathematical model; a final photovoltaic cell mathematical model is obtained through the optimal parameter set, and error verification is carried out on the photovoltaic cell mathematical model; according to the method, firstly, a mathematical model of a photovoltaic cell is constructed, to-be-optimized parameters in the mathematical model are determined, then a fitness function is defined based on multiple error indexes and physical parameter constraints, and then a parameter optimization model adaptive to multi-modal optimization is formed by combining a whale optimization algorithm and a differential evolution operator. And finally, obtaining an optimal parameter set of the photovoltaic cell model.

The invention relates to the technical field of fuel cells, and provides a method and a device for optimizing operation conditions of a proton exchange membrane fuel cell. The method comprises the following steps: simulating the proton exchange membrane fuel cell according to a local dimension reduction single cell model and initial operation conditions of the proton exchange membrane fuel cell to obtain initial effective output power; sequentially increasing each initial parameter value under the initial operation condition to obtain a target operation condition; simulating the proton exchange membrane fuel cell again according to the target operation condition and the local dimensionality reduction single cell model to obtain the target saturation of liquid water of the cathode catalyst layer and the target effective output power; and obtaining a performance evaluation value corresponding to the target operation condition according to the initial effective output power, the target effective output power and the target saturation degree of the liquid water of the cathode catalyst layer, so as to determine the target operation condition corresponding to the performance evaluation value as the optimal operation condition. By adopting the method, the precision of the performance evaluation value can be improved, and the optimization effect of the operation condition is further improved.

The invention relates to the technical field of biology. The invention provides a grass carp brain cell line sensitive to grass carp reovirus type II, which is preserved in China Center for Type Culture Collection on October 19, 2024, and the preservation number is CCTCC NO: C2024101. The constructed grass carp brain cell line shows remarkable stability, and stable passage is successfully achieved for 60 times. And when GCRV-II is inoculated, an atypical cytopathic effect can be presented. In addition, the grass carp brain cell line has the capability of efficiently replicating GCRV-II, and even if blind passage is carried out for three generations, generated cytotoxicity can still cause morbidity and death of the grass carp. Therefore, a stable foundation is built for deeply exploring the pathogenic mechanism of the GCRV-II, developing vaccine development, screening antiviral drugs and the like, research in related fields is promoted to further progress, and a key cell model and a research basis are provided for development of related research and prevention and control strategies of the grass carp reovirus.

The invention provides a micro-fluidic organ chip for three-dimensional cell culture. The micro-fluidic organ chip comprises a cell model loading and culturing unit, a liquid inflow-outflow unit with height difference, and an embedded inverted cone compression ring sealing unit, the cell model loading and culturing unit is constructed by overlapping at least three layers of chips up and down; and the liquid inflow-outflow unit with the height difference is constructed and formed by respectively forming liquid circulation holes which are hermetically connected through the embedded inverted conical compression ring sealing unit on at least three layers of chips. According to the invention, the cell culture space is expanded through the cell model loading and culture unit, high compatibility of cell culture is realized, and the problem of non-uniform concentration of a test substance is eliminated through the liquid inflow-outflow unit with height difference; and high-reliability miniaturized sealing design is realized through the embedded inverted cone pressing ring sealing unit, and the device can be widely applied to culture of cell models and test substance treatment and effect index detection taking toxicological and pharmacological researches as target scenes.

Provided is a mammalian-derived cell model that can be used in anti-aging drugs. The mammalian-derived extraembryonic trophoblast cell model is an early mammalian-derived extraembryonic stage. A change model and an aging evaluation index system for differentiating the cells into the extraembryonic trophoblast cells / organoids can be used for various anti-aging researches and target detection, anti-aging drug detection, screening and identification, research on innovative drugs / natural products, and development of chemicals and / or health foods and additives. Screening and identifying many resistances is helpful. Candidate targets of aging may be further mechanically and functionally validated for future therapeutic use. The screening of anti-aging drugs or natural products will produce huge economic and social benefits.

The invention provides kelp fucoidin with low molecular weight and high anti-anaphylaxis as well as a preparation method and application thereof, and belongs to the technical field of food processing. The preparation method comprises the following steps: mixing kelp fucoidin with water to obtain a kelp fucoidin solution; and mixing the kelp fucoidin solution with H2O2 and Vc, reacting, adjusting the pH value, dialyzing and freeze-drying to obtain the kelp fucoidin with low molecular weight and high anti-allergic property. The H2O2-Vc degradation system is innovatively applied to preparation of the low-molecular-weight kelp fucoidin and improvement of the anti-allergic activity of the low-molecular-weight kelp fucoidin. In the degradation process, the chemical composition of the fucoidin is partially changed, but monosaccharide types and functional groups are not influenced. Through multiple ways of cell model and animal model experiments, the degraded laminaria fucoidin is more prominent in anti-allergic activity. Technical support is provided for development of seaweed-derived anti-allergic functional active substances, and high-valued utilization of kelp resources in the fields of functional food and medicines is further promoted.

The invention discloses an egg source calcium absorption promoting polypeptide as well as a preparation method and application thereof, and belongs to the technical field of polypeptides. The egg source calcium absorption promoting polypeptide comprises phosvitin source polypeptide or shell membrane source polypeptide; the amino acid sequence of the phosvitin source polypeptide is LSKIWGR, and the amino acid sequence of the shell membrane source polypeptide is GPAGFPGAP. Two egg source polypeptides LSKIWGR and GPAGFPGAP are screened and identified for the first time, a physicochemical activity binding cell model is adopted for evaluating the calcium absorption promoting function of the polypeptides, and it is found that the two polypeptides not only can be efficiently bound with calcium ions, but also have certain capacity of improving the transcellular membrane transport activity of calcium elements, and can promote absorption of the calcium ions. The two egg source polypeptides provided by the invention have the potential of being developed and prepared into related products for supplementing calcium and / or promoting calcium absorption.

The invention discloses a method for treating chicken chronic respiratory diseases by using MG-sEV-miRNA-200, and belongs to the technical field of exosomes, and the method comprises the following steps: step 1, constructing an MG infected cell model, and carrying out fluorescence quantitative analysis on gga-miR-200a-3p in cells / exosomes in different time periods of infection; step 2, carrying out overexpression on the miR-200 in the ACE II cells, and extracting an exosome (MG-sEV-miRNA-200) rich in the miR-200; step 3, constructing a cell model for exosome treatment, and carrying out RNA-seq sequencing to identify an exosome treatment target and a pathway rich in gga-miR-200a-3p; step 4, constructing a chick embryo model infected by MG, and injecting MG-sEV-miRNA-200 to intervene in respiratory diseases caused by MG; 5, evaluating the curative effect; mG-sEV is taken as a medium, miRNA can be protected from being influenced by RNA enzyme activity, the gga-miR-200a-3p-rich MG-sEV has the advantages of low immunogenicity, low cytotoxicity, relatively high stability, relatively high biocompatibility and the like, and the MG-sEV rich in gga-miR-200a-3p can be used for remarkably relieving lung inflammation injury caused by MG infection and reducing the bacterium carrying amount of MG in lung tissues; the method has a wide application prospect in the treatment of chicken chronic respiratory diseases.

The invention discloses a three-dimensional woven composite material reverse design method based on a generative adversarial neural network. The method comprises the following steps: selecting three-dimensional woven composite material samples of different process structures through super-Latin sampling; a Python script is combined with TexGen textile modeling software to generate a three-dimensional woven composite material unit cell model, and Abaqus is imported to calculate the mechanical property of the material; normalized coding is carried out on the process structure of the three-dimensional woven composite material, mechanical properties of the material are combined to serve as a data set, a generative adversarial neural network is trained, and hyper-parameters are optimized through Bayesian. According to the method, mapping between the technological parameters and the mechanical properties of the three-dimensional woven composite material is established through the generative adversarial neural network model, the design, research and development efficiency of the three-dimensional woven composite material can be improved by more than 50%, and the structural design accuracy and the performance prediction accuracy of the three-dimensional woven composite material are improved.

The invention discloses a piezoelectric ceramic fatigue life analysis method based on macro and micro multi-scale. The method comprises the following steps: firstly, automatically establishing a microcosmic unit cell model based on a Python script, realizing automatic assignment of material parameters and application of periodic boundary conditions, and calculating microcosmic unit cell homogenization mechanical parameters; then constructing a macroscopic driver model, inputting microscopic parameters as material attributes, and performing macroscopic fatigue life prediction and damage distribution simulation in combination with a Miner linear damage accumulation theory; and finally, through macro-micro coupling analysis, transmitting the strain of the macroscopic maximum stress unit to the micro model, and researching the intergranular stress distribution characteristics and the interface damage evolution law of the piezoelectric ceramic. According to the method, a multi-scale modeling strategy is adopted, cross-scale fatigue performance prediction from the micro grain scale to the macro structure scale is achieved, and an effective means is provided for reliability design and service life evaluation of the piezoelectric ceramic driver.

The invention belongs to the technical field of biochemistry, and particularly relates to a DR8 cyclic peptide analogue and application thereof. The structure of the DR8 cyclopeptide analogue is R1-dAsp-His-Asn-Cys-Pro-Gln-dAla-Cys-CONH2, in the structural formula, R1 is a chain alkyl group with 1 to 30 carbon atoms, and a fourth amino acid and an eighth amino acid are subjected to cyclization through the chain alkyl group. The biological activity of the DR8 cyclic peptide analogue is researched, and an in-vitro cell model and an in-vivo animal model of renal fibrosis are constructed to evaluate indexes such as in-vitro anti-fibrosis activity, inhibition effect and in-vivo treatment effect of the DR8 cyclic peptide analogue. And the cytotoxicity, serum stability and pharmacokinetics of the DR8 cyclopeptide analogue are researched. Results show that compared with the polypeptide DR3penA, the DR8 cyclic peptide analogue obtained by the invention has better activity indexes and stability in all aspects, and can be used for preparing medicines for preventing renal fibrosis diseases.

The invention belongs to the technical field of biology, and particularly relates to a porcine epidemic diarrhea virus broad-spectrum neutralizing antibody and application thereof. A porcine epidemic diarrhea virus broad-spectrum neutralizing antibody 6G2 is screened, the virus neutralizing activity of the antibody is evaluated through a cell model, and it is found that the antibody can effectively block infection of G1a, G1b, G2a, G2b, G2c and S-INDEL (porcine epidemic diarrhea virus) gene subtype strains with high epidemic rate on cells. Further, animal infection experiments of pigs prove that the neutralizing antibody provided by the invention has good prevention and treatment effects on the porcine epidemic diarrhea virus, and has important significance on improving the capability of preventing and controlling porcine epidemic diarrhea in a pig farm.

The invention belongs to the technical field of microorganisms, and particularly relates to an oligopeptide from lactobacillus plantarum extracellular vesicles and application of the oligopeptide. Specifically, the lactobacillus plantarum extracellular vesicles can recover the skin barrier; the expression of proinflammatory factors in a cell inflammation model can be reduced, and a relatively good anti-inflammatory effect is achieved. Metabolome analysis shows that effective substances having effects on skin barrier injury comprise oligopeptide KLQ; kLQ can regulate skin barrier related gene expression in the SDS-induced damaged cell model; and the expression of skin inflammation-related genes in the SDS-induced damaged cell model is regulated, so that the SDS-induced SDS-induced damaged cell model can be used as a medicine for treating skin barrier injury.

The invention aims to provide a chlorella pyrenoidosa peptide with an effect of preventing alcoholic liver injury, which is prepared by the following steps: crushing chlorella pyrenoidosa, mixing with water, freezing and thawing, and performing ultrasonic treatment to prepare homogenate; alkaline protease is added for enzymolysis, and then flavourzyme is added for enzymolysis; and after the enzymolysis product is adsorbed by activated carbon, the supernate is adsorbed by using an SP Sephadex C25 cation exchange medium. The amino acid sequence of the chlorella pyrenoidosa peptide is PSEQ ID NO: 1-5. The chlorella pyrenoidosa peptide provided by the invention is obtained by enzymolysis, adsorption and cation exchange chromatography technologies, and is verified by a HepG2 cell model to obviously relieve alcoholic liver injury. The raw materials are natural and safe, the process is accurate and efficient, and industrialization potential is achieved. Meanwhile, the peptide is suitable for development of functional food, health care products and medicines, and a low-toxicity and sustainable liver protection solution is provided.

The invention belongs to the technical field of biology, and particularly relates to a human metapneumovirus mouse adapted strain and application thereof. Compared with a wild strain, the strain has higher susceptibility to animals; the strain can be used for treating BALB / c mice and can cause typical clinical symptoms and even death, for example, after the strain infects the BALB / c mice, the disease symptoms are obvious, the weight of 83% infected mice is severely reduced to 75% or below and reaches the humanity killing standard, and the death rate reaches 50% or above 4 days after the strain infects the BALB / c mice; the strain can be used for constructing a cell model or an animal model of human metapneumovirus infection or diseases caused by the human metapneumovirus infection, and is further used for screening drugs for preventing and / or treating the human metapneumovirus infection or the diseases caused by the human metapneumovirus infection. In addition, the strain can also be used for preparing medicines for preventing and / or treating human metapneumovirus infection or diseases caused by the human metapneumovirus infection, preparing antibodies or antiserum for resisting the human metapneumovirus and the like.

The invention belongs to the technical field of medicines, and discloses application of hyperforin in preparation of a medicine for preventing or treating amyotrophic lateral sclerosis. The hyperforin can improve the survival rate of stable transfection cell lines of amyotrophic lateral sclerosis related cell models (SOD1G93A / TDP43M337V), and the hyperforin is prompted to play a protective role in the two cell models. After the hyperforin is given, the expression levels of HO-1 and Drp1 proteins in SOD1G93A cells are increased, which indicates that the hyperforin has certain antioxidant activity; and mitochondrial division in cells can be inhibited, so that cell apoptosis is inhibited and retarded, and a protection effect is achieved. Hypericin can help to improve the SOD1G93A cell morphology and promote synaptic generation, and it is prompted that hypericin has a positive effect on maintaining normal functions of cells. In conclusion, the hyperforin plays a protective role in an SOD1G93A cell model and a TDP43M337V cell model, and the mechanism of the hyperforin is possibly related to the antioxidant activity, the mitochondrial function improvement and the like of the hyperforin.

The invention discloses a construction method of a trophoblast organ-like model, the trophoblast organ-like model and application thereof, and relates to the technical field of cell model.The construction method comprises the steps that placenta villus tissue is aseptically separated, gradient separation and purification are conducted to obtain extraembryonic early cells, the extraembryonic early cells are mixed with matrigel, and the trophoblast organ-like model is obtained; and carrying out 3D culture by using a culture medium containing early-stage and later-stage differentiation compositions to obtain the organ-like model with various trophoblast cell lineages. Based on the model, placenta toxicity research is carried out on pregnancy drugs, and the research specifically comprises the degree of 3D organoid form destruction after drug treatment, organoid activity and the expression condition of trophoblast marker genes. Compared with a traditional two-dimensional cell model, the placental toxicity detection method has the advantages that the placental toxicity of the medicine is detected by taking the trophoblast-like organ as the model, the accuracy is higher, the predictability is higher, and the influence of the medicine on human placental tissues is accurately reflected.

The invention relates to the technical field of biology, and aims to provide a zinc ion-containing influenza A virus culture medium and a preparation method thereof. The culture medium contains a serum-free cell culture medium, water, a PBS (Phosphate Buffer Solution), bovine serum albumin with the final concentration of 20g / L, TPCK-Trypsin with the final concentration of 2mu g / mL and zinc ions with the final concentration of 0.009-0.015 mmol / L. According to the invention, zinc ions which are originally considered to generate a virus inhibition effect under a relatively low concentration condition can be used for promoting virus replication after the use concentration range is adjusted; therefore, a brand-new culture medium which can be applied to research on common cell models and virus in-vitro culture models of the influenza A virus is provided, and a new solution is provided for optimizing a vaccine production process. The culture medium is simple in formula and convenient to prepare; all the raw material components are common consumables in laboratories, and the required liquid components can be prefabricated in advance and refrigerated for later use; and large-scale vaccine production can be supported.

The invention provides a TF and Her2 targeting bispecific antibody coupling drug as well as a preparation method and application thereof, and belongs to the technical field of biological drug preparation. The bispecific antibody provided by the invention can target TF and / or Her2 antigens in tumor cells, and has the advantages of high stability, easiness in expression, purification and coupling and the like. The bispecific antibody can be specifically combined with a tumor surface antigen and internalized into tumor cells, so that the tumor cells can be specifically killed. The bispecific antibody coupling drug prepared on the basis of the bispecific antibody has a good tumor inhibition effect in a cell model and an animal model, is nontoxic and harmless to animals, and has an excellent cancer treatment potential.