1147 results about "Cell model" patented technology

The present invention relates to a computer-implemented method and system for optimizing geometric configurations within thermal energy transfer device comprising at least one thermal energy exchange channel to enhance performance. The method involves obtaining target design criteria for a physical object and defining a unit cell dimensioned such that the thermal energy transfer channel is formed by a matrix of N1 x N2 identical unit cells. Shape optimization is performed at the unit cell level by iteratively modifying the boundary surfaces of solid structures within a unit cell based on criteria including performance. Optimized design parameters are outputted and transferred to production equipment for creating an optimized thermal energy transfer device. This device comprises a matrix of structures arrayed in at least one dimension, derived from optimized unit cell models.

The present application discloses a cell culture unit, device, application and culture method, wherein the cell culture unit includes a substrate, a culture chamber, a liquid storage chamber and a flow channel chamber are formed in the substrate, and the culture chamber and the liquid storage chamber have an opening at the top of the substrate, and the two ends of the flow channel chamber are connected to the culture chamber and the liquid storage chamber respectively, and the flow channel chamber includes a first port portion connected to the liquid storage chamber and a second port portion connected to the culture chamber, and the flow channel chamber is also formed with a constriction portion between the first port portion and the second port portion, and the opening area of ​​the constriction portion is smaller than the opening area of ​​the first port portion and the second port portion. The technical solution of the present application can avoid the impact on the biological sample caused by too fast flow rate, resulting in the cells in the liquid gel being dispersed by the culture medium, and increase the time for the culture medium to infiltrate the biological sample to fully infiltrate the biological sample, thereby being able to improve the simulation degree of the cell model finally obtained and improve the success rate of culture.

Techniques are described for using machine learning to extract physics-based cell model parameters from battery charge data provided by the battery module of the battery management system. This data may be processed via machine learning techniques in order to periodically update or adapt a physics-based model that may capture cell electrical dynamics and degradation mechanisms. The techniques may utilize a “digital twin” to adaptively charge the battery while preserving the life of the cells.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine prescription efficacy quantification method and system.The method comprises the steps that chemical component spectrums, pharmacodynamic substance contents and biological activity indexes of traditional Chinese medicines forming a prescription are obtained; constructing a multi-target regulation and control network by combining a traditional Chinese medicine-target interaction database according to the chemical component spectrum, the pharmacodynamic substance content and the biological activity index, identifying a core efficacy pathway cluster by adopting a Louvain algorithm, and calculating a pathway activation intensity value by adopting a pathway target membership weighting model; according to the core efficacy pathway cluster, establishing a mapping relation between the core efficacy pathway cluster and traditional Chinese medicine efficacy terms through ontology and semantic mapping, and according to pathway activation intensity values, generating a prescription efficacy intensity vector through a weighted aggregation model; according to the efficacy intensity vector of the prescription, verifying and correcting the network weight through an in-vitro cell model, and generating the efficacy quantification of the prescription in combination with the clinical syndrome calibration coefficient. Therefore, the problems of single data dimension and insufficient quantification result accuracy in the prior art are solved.

The invention relates to the field of biological imaging, and particularly discloses an in-vitro cell microenvironment dynamic monitoring method for two-photon frequency domain fluorescence lifetime imaging, which comprises the following steps: S1, by using a two-photon frequency domain fluorescence lifetime imaging TP-FD-FLIM technology, losslessly representing microenvironment change through fluorescence lifetime parameters, and monitoring oxidative stress reaction and neutrophil migration behavior in real time, analyzing the effect of the associated compound in combination with an in-vitro cell model; and S2, based on the physical basis of FD-FLIM, establishing a frequency domain fluorescence lifetime signal processing and analysis system under periodic excitation, analyzing the dynamic correlation characteristics of the phase delay phi and the modulation depth M of a fluorescence signal relative to exciting light, and carrying out quantitative backstepping on molecular lifetime parameters. According to the in-vitro cell microenvironment dynamic monitoring method for two-photon frequency domain fluorescence lifetime imaging provided by the invention, neuroinflammatory response can be observed in a lossless and real-time manner on the subcellular scale.

The invention belongs to the technical field of biological medicines, and discloses an angiotensin I converting enzyme inhibitory peptide. According to the present invention, the action mechanisms of two ACE inhibitory peptides H5 (LIIPQH, 719.85 Da) and H6 (LIIPEH, 720.85 Da) extracted, separated and purified from rice wine residue are researched, and the in vitro activity of the ACE inhibitory peptides is researched through an EA.hy926 cell model, such that the two ACE inhibitory peptides can provide the activity effect by promoting the release of NO, the expression of eNOS and SOD, and inhibiting the release secretion of ET-1 and the release of ROS; meanwhile, the cells are subjected to transcriptomics analysis, and the pathway mechanism of polypeptide action is defined. The ACE inhibitory peptide is expected to serve as a potential antihypertensive drug or a functional food ingredient, the high-value utilization of the rice wine residues is improved, and a new application way is provided for the food-borne ACE inhibitory peptide in the aspect of treating hypertension.

The invention belongs to the field of lattice structure design, and particularly discloses a continuous fiber reinforced composite material strength Poisson's ratio adjustable design method and system.The design method comprises the steps that the Poisson's ratio structure type needed by a continuous fiber reinforced composite material component to be prepared is determined, the Poisson's ratio structure type comprises a zero Poisson's ratio structure, a positive Poisson's ratio structure or a negative Poisson's ratio structure; according to the method, regular hexagonal unit cell models and hourglass-shaped hexagonal unit cell models are constructed, and according to the Poisson's ratio structure type, a plurality of regular hexagonal unit cell models and / or a plurality of hourglass-shaped hexagonal unit cells are utilized to construct a corresponding honeycomb porous structure model, so that the Poisson's ratio adjustable design of the continuous fiber reinforced composite material is realized. By means of the method, standardized design of material structures of high-strength, light-weight, vibration-reducing and energy-absorbing parts needed by various parts in different application fields can be achieved.

Provided is the use of a conjugated cholic acid, tryptophan-conjugated bile acids (TrpCA) or a TrpCA-producing strain for preventing and / or treating a metabolic disease. The present disclosure relates to the use of tryptophan-conjugated cholic acid or its producing bacteria strain in the preparation of a composition for preventing and / or treating a metabolic disease, or for promoting GLP-1 and / or insulin production. The inventors have discovered for the first time that the tryptophan-conjugated cholic acid can significantly promote the secretion of GLP-1 in both in vivo animal model and in vitro cell model, thereby reducing weight, lowering blood glucose and relieving symptoms of non-alcoholic fatty liver disease. Moreover, colonization of the selected probiotics can also have the same metabolic improvement effects.

The invention discloses a human-derived ampulla cancer cell line DPC-X3 and application thereof, and belongs to the field of microbial animal cell lines. The human-derived kettle belly cancer cell line is named as a human-derived kettle belly cancer cell line DPC-X3, and the preservation number is CCTCC (China Center For Type Culture Collection): C2024317. The human-derived ampulla cancer cell line DPC-X3 can be applied to establishment of a cell model for occurrence, development or metastasis of ampulla cancer. The human-derived ampulla cancer cell line DPC-X3 can be applied to cell models for researching the differentiation mechanism, cell morphology and dysfunction and tumor infiltration and metastasis mechanism of ampulla cancer and guiding clinical comprehensive diagnosis and treatment. The human-derived ampulla cancer cell line DPC-X3 can be applied to research on the occurrence mechanism of the ampulla cancer and screening of drugs for preventing and treating the ampulla cancer. The ampulla cancer cell line DPC-X3 can be applied to establishment of an ampulla cancer animal model.

The invention discloses a breast adenoma B3 type source cell strain as well as construction and application thereof, the breast adenoma B3 type source cell strain is named as human breast adenoma cell Hthy2301 Homo sapiens and is preserved in China Center for Type Culture Collection on March 14, 2024, and the preservation number is CCTCC NO: C202478. The human thymoma cell line is stable in character, can be passaged in vitro for a long time, and has high proliferation activity. The human thymoma cell line disclosed by the invention can be used as an effective cell model, and a new experimental model can be provided for deeply researching the occurrence, development and transfer mechanisms of thymoma, the drug resistance mechanism, drug screening, drug effect evaluation and the like.

The invention discloses a lubricant adsorption and friction regulation and control method based on molecular dynamics simulation, which comprises the following steps: S1, constructing a lubricant molecule and friction pair metal unit cell model, generating topological structure information based on a minimum structure unit, and constructing a lubrication system model with a sandwich structure; s2, giving force field parameters to the lubrication system model, and performing geometric structure optimization and dynamic relaxation; s3, giving surface charges to the upper and lower friction pairs for targeted adsorption simulation of the lubricant; and S4, applying uniformly distributed contact pressure and constant sliding speed to the upper friction pair to perform friction simulation of the lubrication system. According to the lubricant adsorption and friction regulation and control method based on molecular dynamics simulation, a targeted adsorption strategy of a solid-liquid interface is established, the microcosmic adsorption process of the lubricant is reproduced, controllable regulation of friction characteristics is achieved, the human resource cost in actual experiments is reduced, and manual operation errors are reduced.

The invention discloses a rapid equalizing charging method for a lithium ion battery pack, and belongs to the field of battery charging. The method comprises the following steps: step 1, establishing a first-order enhanced equivalent circuit model, constructing an aging model in combination with an Arrhenius equation, and comprehensively establishing an electric heating aging fusion model; 2, constructing a battery pack model through series connection based on a single battery model of the electrothermal aging fusion model; 3, configuring a deep reinforcement learning environment on the basis of the electrothermal aging coupling model, wherein the deep reinforcement learning environment is used for simulating the real state of interaction between a deep deterministic policy gradient algorithm (DDPG) and the battery pack in deep reinforcement learning; 4, a deep reinforcement learning Actor network, a Critic network and a reward function are constructed, and the target of the reward function is defined as the shortest charging time of the battery pack, SOC (state of charge) balance and minimum aging loss so as to support operation and result evaluation of a DDPG algorithm; and 5, training the model to obtain the optimal fast equalization charging current.

The invention belongs to the technical field of microbial medicine, and provides a primary multidrug resistance human far-end bile duct cancer cell line CBC3T-6 Homo sapiens and application thereof.The human far-end bile duct cancer cell line CBC3T-6 Homo sapiens is separated from differentiated adenocarcinoma in a far-end bile duct, the human far-end bile duct cancer cell line CBC3T-6 Homo sapiens is already subcultured to 50 generations at present, the long-term in-vitro culture character is stable, and on the premise that the biological characteristics of bile duct cancer are reserved, the human far-end bile duct cancer cell line CBC3T-6 Homo sapiens can be used for preparing the primary multidrug resistance human far-end bile duct cancer cell line CBC3T-6 Homo sapiens. The compound has the characteristics of short multiplication time, high mouse subcutaneous tumor formation rate and the like, and has different degrees of drug resistance to gemcitabine, 5-fluorouracil, oxaliplatin and cis-platinum in first-line anti-cancer drugs for bile duct cancer. The method not only enriches an extrahepatic bile duct cancer cell bank, provides a new cell model for basic research and clinical drug research and development of bile duct cancer, but also provides powerful scientific research data for research developed based on the genetic background of Chinese population. Therefore, the primary multidrug resistant cell line has higher scientific research and clinical application values.

The invention discloses a modeling method and a processing device for a lattice structure gear filled with a three-period minimal curved surface, and the method comprises the following steps: writing an implicit function equation of the three-period minimal curved surface by using Matlab numerical analysis software, and generating an stl format model; using Geomagic reverse modeling software to perform reverse modeling on the three-period minimal curved surface cell elements to produce an stp format model; using Matlab numerical analysis software to generate data points, and importing Proe software to generate a gear model; after operations of mirroring, arraying and the like are carried out on the curved surface cell element model by using SolidWorks, a lattice structure is obtained by using a curved surface thickening command; according to the method, SolidWorks is used for dividing the gear into three parts, namely a gear tooth design area, a hub design area and a spoke design area, the spoke design area intersects with a lattice structure Boolean and then is combined with the gear tooth area Boolean and the hub area Boolean, and therefore the lattice structure gear filled with the three-period minimal curved surface is constructed, and a new thought and method are provided for lightweight design of the gear.

The invention relates to application of cembratrienol in preparation of a medicine for preventing and / or treating psoriasis, and belongs to the technical field of biological medicine. The invention provides an application of cembratrienol in preparation of a medicine for preventing / or treating psoriasis. Researches find that cembratrienol can significantly inhibit secretion of inflammatory factors in a psoriasis-like cell model induced by M5; moreover, research finds that cembratrienol can significantly improve the skin lesion condition of an imiquimod-induced psoriasis mouse model, improve the weight loss degree of the psoriasis mouse model, relieve the pruritus condition of the psoriasis mouse model, improve the skin inflammation of the psoriasis mouse model, repair the skin barrier of the psoriasis mouse model, and improve the curative effect of the psoriasis mouse model. The body immune response of the psoriasis mouse model is reduced. Therefore, cembratrienol has a great application prospect in preparation of the medicine for preventing / treating psoriasis.

The invention relates to the technical field of medicines, in particular to a construction method and application of a photothermal conversion platform for treating osteoarthritis by regulating synovial macrophage polarization. According to the present invention, the M2 membrane-structured black phosphorus selenium carbon body (M2-BPSeC) photo-thermal nano-platform is innovatively provided, the triple functions of bionic targeting delivery, precise thermal control intervention and immune microenvironment remodeling are integrated, and the excellent synergistic treatment effect is represented in the in-vitro cell model and the OA animal model. The technology breaks through the technical bottleneck that traditional treatment is insufficient in targeting and single in intervention dimension, precise functional coupling of the physical thermal effect and immunoregulation is achieved for the first time, and a brand new strategy of targeting-temperature control-immunity integration is provided for osteoarthritis treatment. The efficient and controllable preparation process and the remarkable multi-dimensional treatment effect open up a new path for cartilage regeneration and immune balance regulation and control.

The invention provides an application of puerarin in treatment of periodontitis. The molecular mechanism of puerarin (Pue) for treating periodontitis (PDS) is verified through a plurality of in-vitro and in-vivo experimental studies. In PDS mice, Pue treatment relieves periapical tissue inflammation and bone destruction, and promotes osteogenic differentiation and autophagy of periapical tissues. In a PDS cell model, Pue treatment promotes osteogenic differentiation and mitochondrial autophagy, inhibits inflammation, maintains mitochondrial membrane potential and mitochondrial dynamic homeostasis, and activates mitochondrial Mfn2. In addition, the mitochondrial autophagy inhibitor eliminates the influence of Pue on a PDS cell model, and Mfn2 silence reverses mitochondrial autophagy enhancement induced by Pue. In-vivo tests verify that Mfn2 knockout counteracts the therapeutic effect of Pue on PDS mice. The invention provides a sufficient basis and an exact molecular mechanism for application of puerarin in treatment of periodontitis.

The invention discloses an RSV infection immortalized tree shrew aorta vascular endothelial cell model, and particularly relates to a method for separating and culturing primary aorta vascular endothelial cells from tree shrew aorta, further establishing an immortalized tree shrew aorta vascular endothelial cell line, taking the cell line as an RSV virus culture medium, and constructing an RSV infection immortalized tree shrew aorta vascular endothelial cell model. Establishing an RSV infected immortalized tree shrew aortic vascular endothelial cell model, and preparing a high-infection-titer RSV virus; the invention provides key support for preparation and detection of RSV virus and construction of a tree shrew in-vivo infection model, provides a stable virus source for development of comparative medical research or vaccine evaluation, and has far-reaching significance for deep exploration of RSV pathogenic mechanism, drug and vaccine evaluation and the like.

The invention discloses a culture medium and a method for performing in-vitro 3D cell model construction on skin tissues. The invention provides a culture medium for constructing a skin tissue in-vitro 3D (three-dimensional) cell model. The culture medium is prepared from an antibacterial and antifungal agent tri-antibody, HEPES, GlutaMax, MEM non-essential amino acid, human recombinant protein EGF, bFGF, HGF, FGF-10, Wnt-3a, Noggin, R-spondin 1, IL-2, IL-15, IGF-I, CHIR99021, SB202190, A83-01, Primocin, N-acetyl-L-cysteine, nicotine, an N2 additive, B27, ITS-X, Y-27632, dexamethasone, calomel and a basal culture medium. The skin tissue in-vitro 3D cell model constructed by the invention has a wide application prospect in the fields of basic scientific research and clinical diagnosis and treatment of skin diseases.

The present invention relates to a versatile microfluidic apparatus (100, 400) and a cell culture chamber attachment thereof. The present invention discloses a high-throughput and versatile multi-compartment organ-on-chip microfluidic apparatus to culture various 3D cell models and mimics the organ model with specific parenchyma and stroma. The cell culture chamber attachments (11) are replaceable and based on the variant of the 3D cell or tissue models. The apparatus (100, 400) is suitable for a variety of 3D cell models such as spheroid, biopsy tissue, organoid, hydrogel embedded tissue, 3D patterned, and 3D bio-printed culture models.

The invention relates to the field of photovoltaic technology, in particular to a photovoltaic cell model parameter extraction method and related products, and the method comprises the steps: building a photovoltaic cell mathematical model, and determining to-be-optimized model parameters in the mathematical model; constructing a fitness function of the photovoltaic cell mathematical model; constructing a parameter optimization model based on a whale optimization algorithm and a differential evolution operator, and taking a fitness function as a target function; executing the parameter optimization model, and obtaining an optimal parameter set corresponding to model parameters to be optimized in the mathematical model; a final photovoltaic cell mathematical model is obtained through the optimal parameter set, and error verification is carried out on the photovoltaic cell mathematical model; according to the method, firstly, a mathematical model of a photovoltaic cell is constructed, to-be-optimized parameters in the mathematical model are determined, then a fitness function is defined based on multiple error indexes and physical parameter constraints, and then a parameter optimization model adaptive to multi-modal optimization is formed by combining a whale optimization algorithm and a differential evolution operator. And finally, obtaining an optimal parameter set of the photovoltaic cell model.

The invention relates to a design method of a CAR-T cell model targeting human TROP2 and application of the CAR-T cell model in triple negative breast cancer. The method comprises the following steps: obtaining protein sequences of a light chain and a heavy chain by utilizing a sequence of a targeting drug of the human TROP2; comparing the protein sequences of the light chain and the heavy chain with the sequence of the drug protein, and removing the same sequence part to obtain a variable protein sequence; transforming the variable protein sequence into a base sequence to respectively obtain base sequences of a light chain variable region and a heavy chain variable region, and adding a signal peptide and a connecting region to synthesize two base sequences to obtain a human TROP2 antibody sequence fragment; the preparation method comprises the following steps: synthesizing plasmids carrying a human TROP2 antibody sequence fragment by taking an SFG retroviral vector as a skeleton; and enabling the human T cells to express a target gene through transfection. In the embodiment, the nucleic acid sequence of the human TROP2 antibody as shown in SEQ ID NO.1 and SFG are used as carriers, a retrovirus transfection system is used for constructing a TROP2. CAR-T cell model, the functionality of the TROP2. CAR-T cell on the triple negative breast cancer is defined, and a new method is provided for clinical treatment of the triple negative breast cancer.

The invention relates to the technical field of fuel cells, and provides a method and a device for optimizing operation conditions of a proton exchange membrane fuel cell. The method comprises the following steps: simulating the proton exchange membrane fuel cell according to a local dimension reduction single cell model and initial operation conditions of the proton exchange membrane fuel cell to obtain initial effective output power; sequentially increasing each initial parameter value under the initial operation condition to obtain a target operation condition; simulating the proton exchange membrane fuel cell again according to the target operation condition and the local dimensionality reduction single cell model to obtain the target saturation of liquid water of the cathode catalyst layer and the target effective output power; and obtaining a performance evaluation value corresponding to the target operation condition according to the initial effective output power, the target effective output power and the target saturation degree of the liquid water of the cathode catalyst layer, so as to determine the target operation condition corresponding to the performance evaluation value as the optimal operation condition. By adopting the method, the precision of the performance evaluation value can be improved, and the optimization effect of the operation condition is further improved.

The invention relates to the technical field of biology. The invention provides a grass carp brain cell line sensitive to grass carp reovirus type II, which is preserved in China Center for Type Culture Collection on October 19, 2024, and the preservation number is CCTCC NO: C2024101. The constructed grass carp brain cell line shows remarkable stability, and stable passage is successfully achieved for 60 times. And when GCRV-II is inoculated, an atypical cytopathic effect can be presented. In addition, the grass carp brain cell line has the capability of efficiently replicating GCRV-II, and even if blind passage is carried out for three generations, generated cytotoxicity can still cause morbidity and death of the grass carp. Therefore, a stable foundation is built for deeply exploring the pathogenic mechanism of the GCRV-II, developing vaccine development, screening antiviral drugs and the like, research in related fields is promoted to further progress, and a key cell model and a research basis are provided for development of related research and prevention and control strategies of the grass carp reovirus.

The invention provides a traditional Chinese medicine composition. Compared with the prior art, the traditional Chinese medicine composition has the advantages that the traditional Chinese medicines are reasonably matched, so that all the components have the effect of synergistically improving insomnia, and uncomfortable symptoms such as exhaustion, cognitive competence impairment and irritability of insomnia patients are improved; meanwhile, a transcriptome sequencing technology is adopted, the effect of the traditional Chinese medicine composition on signal channels of patients with insomnia and mild and moderate senile dementia is analyzed and studied, and it is found that the traditional Chinese medicine composition can remarkably reverse disease-related channels on disease-related cell models, remarkably reverse insomnia disorder channels in neuronal cells and microglia cells, and remarkably reverse insomnia disorder channels in neuronal cells and microglia cells. Disorder pathways of mild and moderate senile dementia diseases are obviously reversed in neuronal cells and vascular endothelial cells; therefore, the traditional Chinese medicine composition can be used for improving insomnia and preventing and treating mild and moderate senile dementia, and the traditional Chinese medicine composition is prepared from medicinal and edible medicinal materials and is safe and reliable.

The invention provides a micro-fluidic organ chip for three-dimensional cell culture. The micro-fluidic organ chip comprises a cell model loading and culturing unit, a liquid inflow-outflow unit with height difference, and an embedded inverted cone compression ring sealing unit, the cell model loading and culturing unit is constructed by overlapping at least three layers of chips up and down; and the liquid inflow-outflow unit with the height difference is constructed and formed by respectively forming liquid circulation holes which are hermetically connected through the embedded inverted conical compression ring sealing unit on at least three layers of chips. According to the invention, the cell culture space is expanded through the cell model loading and culture unit, high compatibility of cell culture is realized, and the problem of non-uniform concentration of a test substance is eliminated through the liquid inflow-outflow unit with height difference; and high-reliability miniaturized sealing design is realized through the embedded inverted cone pressing ring sealing unit, and the device can be widely applied to culture of cell models and test substance treatment and effect index detection taking toxicological and pharmacological researches as target scenes.

Provided is a mammalian-derived cell model that can be used in anti-aging drugs. The mammalian-derived extraembryonic trophoblast cell model is an early mammalian-derived extraembryonic stage. A change model and an aging evaluation index system for differentiating the cells into the extraembryonic trophoblast cells / organoids can be used for various anti-aging researches and target detection, anti-aging drug detection, screening and identification, research on innovative drugs / natural products, and development of chemicals and / or health foods and additives. Screening and identifying many resistances is helpful. Candidate targets of aging may be further mechanically and functionally validated for future therapeutic use. The screening of anti-aging drugs or natural products will produce huge economic and social benefits.

The invention provides kelp fucoidin with low molecular weight and high anti-anaphylaxis as well as a preparation method and application thereof, and belongs to the technical field of food processing. The preparation method comprises the following steps: mixing kelp fucoidin with water to obtain a kelp fucoidin solution; and mixing the kelp fucoidin solution with H2O2 and Vc, reacting, adjusting the pH value, dialyzing and freeze-drying to obtain the kelp fucoidin with low molecular weight and high anti-allergic property. The H2O2-Vc degradation system is innovatively applied to preparation of the low-molecular-weight kelp fucoidin and improvement of the anti-allergic activity of the low-molecular-weight kelp fucoidin. In the degradation process, the chemical composition of the fucoidin is partially changed, but monosaccharide types and functional groups are not influenced. Through multiple ways of cell model and animal model experiments, the degraded laminaria fucoidin is more prominent in anti-allergic activity. Technical support is provided for development of seaweed-derived anti-allergic functional active substances, and high-valued utilization of kelp resources in the fields of functional food and medicines is further promoted.

The invention provides a sound insulation performance optimization method of a multilayer film type acoustic metamaterial based on an orthogonal experiment. And a new thought is provided for realizing the design of the low-frequency broadband sound insulation material. Firstly, an MAM unit cell model based on a simplified spider topological structure is designed in combination with an existing polymorphic anti-resonance cooperative type MAM, a three-layer structure is established based on the model, and improvement of the sound insulation index and widening of the sound insulation bandwidth are achieved. Secondly, analyzing the influence of the number of resonators on the sound insulation performance in combination with sound transmission loss (STL), and revealing the sound insulation mechanism through intrinsic modes of different numbers of resonators in combination with the STL. Thirdly, analyzing the sound insulation performance of the structure when the number of the resonators in each layer is different, and screening out the structure with the optimal sound insulation performance; and finally, performing multi-factor analysis on the sound insulation performance by using an orthogonal experiment to obtain an optimal combination of a plurality of parameters, verifying the accuracy, and further optimizing the sound insulation performance.

The invention discloses an egg source calcium absorption promoting polypeptide as well as a preparation method and application thereof, and belongs to the technical field of polypeptides. The egg source calcium absorption promoting polypeptide comprises phosvitin source polypeptide or shell membrane source polypeptide; the amino acid sequence of the phosvitin source polypeptide is LSKIWGR, and the amino acid sequence of the shell membrane source polypeptide is GPAGFPGAP. Two egg source polypeptides LSKIWGR and GPAGFPGAP are screened and identified for the first time, a physicochemical activity binding cell model is adopted for evaluating the calcium absorption promoting function of the polypeptides, and it is found that the two polypeptides not only can be efficiently bound with calcium ions, but also have certain capacity of improving the transcellular membrane transport activity of calcium elements, and can promote absorption of the calcium ions. The two egg source polypeptides provided by the invention have the potential of being developed and prepared into related products for supplementing calcium and / or promoting calcium absorption.