203 results about "HaCaT" patented technology

The present invention provides a Lactiplantibacillus plantarum ( Lactiplantibacillus plantarum ), strain 24, which is deposited in the China General Microbiological Culture Collection Center, deposit number: CGMCC No. 31278. The Lactiplantibacillus plantarum 24 of the present invention can secrete sphingomyelinase to promote the production of ceramide in HaCaT cells; Lactiplantibacillus plantarum 24 or its metabolite or its lysate can up-regulate the expression of key enzymes in the sphingomyelinase and / or ceramide synthesis pathway of skin cells, repair the skin barrier; down-regulate the expression of pro-inflammatory cytokines, inhibit autophagy, and has a good anti-inflammatory effect; at the same time, it also has good antioxidant and whitening effects. The Lactiplantibacillus plantarum 24 or its metabolite or its cell lysate of the present invention can be used in the preparation of products having the above-mentioned effects or functions.

The invention discloses pediococcus pentosaceus CCFM1402 capable of effectively repairing a skin barrier and a self-fermentation metabolite of the pediococcus pentosaceus CCFM1402, and belongs to the technical field of microorganisms and medicines. The pediococcus pentosaceus CCFM1402 provided by the invention has the effect of improving the skin barrier function in both external use and oral administration; the cell activity of damaged HaCaT cells and the expression of the gene level of skin barrier function protein and tight junction protein can be improved by virtue of a thallus lysate and a fermented supernatant (metagen) of the external pediococcus pentosaceus CCFM1402; and the DNFB-induced mouse skin barrier injury can be relieved in the aspects of appearance, skin barrier protein expression and the like by orally taking viable bacteria and postbiotics of the pediococcus pentosaceus CCFM1402. Therefore, the pediococcus pentosaceus CCFM1402 has a huge application prospect in preparation of external or oral medicines for repairing the skin barrier and preparation of food, health care products or cosmetics.

The invention provides a lactobacillus plantarum 24 which is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms, and the preservation number of the lactobacillus plantarum 24 is CGMCC No.31278. The lactobacillus plantarum 24 has the advantages that the lactobacillus plantarum 24 is prepared from lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum, lactobacillus plantarum and lactobacillus plantarum; the plant lactobacillus 24 disclosed by the invention can secrete sphingomyelinase and promote HaCaT cells to generate ceramide; the plant lactobacillus 24 or the metabolite or lysate thereof can up-regulate expression of key enzymes in a synthetic route of skin cell sphingomyelinase and / or ceramide, and repair a skin barrier; expression of proinflammatory cytokines is lowered, autophagy is inhibited, and a good anti-inflammatory effect is achieved; meanwhile, the anti-oxidation and whitening effects are relatively good. The plant lactobacillus 24 or the metabolite or the cell lysate of the plant lactobacillus 24 can be applied to preparation of products with the effects or the effects of the plant lactobacillus 24 and the metabolite or the cell lysate of the plant lactobacillus 24.

The invention relates to application of sea cucumber-derived nano-vesicles in preparation of a medicine for promoting wound repair. The nano-vesicles (SC-EVs) extracted and prepared from sea cucumbers can effectively promote cell migration, scratch repair and tube forming capabilities of human skin epithelial cells (HACAT) and human skin microvascular endothelial cells (HM DEC) and inhibit cell apoptosis, so that the capability of repairing cells is achieved, and particularly the effect of repairing wound surfaces in a high glucose state or diabetes mellitus is achieved. The invention provides a new direction and thought for the application of the sea cucumber-sourced nano vesicles (SC-EVs), provides a brand new drug research and development and clinical treatment direction for wound repair, especially for solving the problem of difficult healing of wounds of patients with high glucose or diabetes, and has extremely high social value and market application prospect.

The invention belongs to the technical field of biological medicines, and particularly relates to an application of a Pucurbita pepo polypeptide Nacrein-LP20 in preparation of a medicine for promoting wound healing and a preparation method of the Pucurbita pepo polypeptide Nacrein-LP20. The amino acid sequence of the Hepu pearl polypeptide Nacrein-LP20 is as shown in SEQ ID NO: 1 (Sequence Identity Number 1). According to the present invention, the Pucurbita pepo active polypeptide Nacrein-LP20 has characteristics of strong DPPH free radical and hydroxyl free radical removing ability and excellent antioxidant activity, can significantly promote HaCaT cell proliferation in the high glucose environment, and can promote migration and repair of HaCaT cells in the high glucose environment; the traditional Chinese medicine composition has a remarkable healing promoting effect on full-cortical resection wound surfaces of normal rats and diabetic rats.

The invention belongs to the technical field of microorganisms, and particularly relates to an extracellular vesicle of phytobacterium plantarum and application of the extracellular vesicle. Specifically, the plant lactobacillus extracellular vesicles can promote HaCaT cells to generate ceramide, up-regulate expression of key genes in a skin ceramide synthesis route and recover a skin barrier; the expression of proinflammatory factors in a cell inflammation model can be reduced, and a relatively good anti-inflammatory effect is achieved. A mouse skin injury model verifies that the EVs has good skin infiltration capacity and can remarkably reduce the skin injury score, relieve skin itch, improve the skin water content and reduce expression of proinflammatory factors in skin tissue and expression of pruritus-related cell factors.

The invention belongs to the field of biological medicine, and particularly relates to an antibacterial peptide for inhibiting staphylococcus aureus and pseudomonas aeruginosa and application of the antibacterial peptide. The antibacterial peptide CAMP648NC is a novel cationic antibacterial peptide, the minimum inhibitory concentrations of the antibacterial peptide CAMP648NC to pathogenic bacteria staphylococcus aureus and pseudomonas aeruginosa are 16 [mu] g / mL and 32 [mu] g / mL respectively, the antibacterial peptide CAMP648NC has extremely low cytotoxicity, and after human skin keratinocytes HaCat and bronchial epithelial cells BEAS-2B are treated by the antibacterial peptide CAMP648NC with the concentration of 128 [mu] g / mL for 24 hours, the survival rates of 110.4% and 87.3% are still kept. Due to the characteristics, the compound shows extremely high medical drug antibacterial value, and alternative medicinal resources are provided for clinically resisting the two human pathogenic bacteria in the future.

The invention relates to a sodium hyaluronate fermentation filtrate as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. Through a two-stage fermentation mode, the fermentation filtrate containing sodium hyaluronate is prepared by recombining bacillus subtilis, and then residual nutrient substances in a culture medium are consumed by inoculating bacillus velezensis, so that the nutrient substances are metabolized into active substances with repairing and strengthening effects on hair; the prepared sodium hyaluronate fermentation filtrate has the effects of repairing scalp and strengthening hair. The sodium hyaluronate filtrate disclosed by the invention can be used for well reducing the content of NO in macrophages (the NO content of cells is increased due to malassezia lysate), can be used for removing active oxygen ROS of HaCaT cells and increasing the total tensile strength of hair, and has the potential effects of relieving scalp, resisting oxidative stress and enhancing the toughness of the hair.

The invention discloses a strain of Blastococcus atamensis YC-1 and application of the strain of Blastococcus atamensis YC-1, and belongs to the field of microbial strains. The streptococcus sp. YC-1 provided by the invention has high antioxidant activity and also has the capability of producing DHHB (docosahexaenoic acid); meanwhile, the YC-1FLE is prepared by adopting absolute ethyl alcohol as a solvent, has no toxicity to HaCaT cells, is relatively high in reducing capacity, has a scavenging effect on DPPH free radicals, ABTS cation free radicals and hydroxyl free radicals, has a tyrosinase inhibition effect and shows good antioxidant and whitening effects; when the YC-1FLE is applied to the sunscreen lotion, the sunscreen coefficient of the sunscreen lotion containing the YC-1FLE is obviously higher than that of a blank control group, which shows that the YC-1FLE has better ultraviolet resistance. Therefore, the strain and the lysate extract of the fermentation product of the strain have huge application potential in the field of cosmetics.

The invention discloses a plant lactobacillus with light aging resistance and an application thereof. The preservation number of the plant lactobacillus cj001 disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No.35419. The fermentation supernatant of the plant lactobacillus cj001 shows good anti-aging activity and in-vitro antioxidant activity, and the growth ability of HaCaT cells is promoted. After the combined fermentation product of the plant lactobacillus cj001 and the lactobacillus paracasei Grx701 is subjected to ultraviolet radiation, the proliferation of HaCaT cells can be promoted, the endogenous antioxidant capacity of human skin cells can be enhanced, the photooxidation damage can be effectively relieved, and the combined fermentation product has a wide application value in the field of preparation of products with antioxidant, anti-aging, anti-ultraviolet damage, anti-photoaging and the like.

The invention discloses an application of Chinese yam derived exosome-like nanoparticles in preparation of skin injury repairing products, the exosome-like nanoparticles are derived from natural medicinal and edible plants, and have no cytotoxicity to skin keratinocytes HaCaT; scratch cell migration experiments are carried out, and it is found that healing can be rapidly promoted, and the anti-inflammatory effect is achieved. The invention further provides a preparation method of the Chinese yam-derived exosome-like nano-particles, Chinese yam tissue raw stock is separated through centrifugal treatment and sucrose gradient centrifugation, the high-purity exosome-like nano-particles are prepared, and the method is simple in technological process and easy to implement.

The invention belongs to the technical field of biological medicine, and discloses application of cornu cervi degelatinatum extract in strengthening a skin barrier. The cornu cervi degelatinatum extract disclosed by the invention can promote proliferation of HacaT cells and expression of tight junction protein Claudin-1, also has better capabilities of removing hydroxyl free radicals, DPPH free radicals and superoxide anions and an effect of promoting wound healing, and can protect a skin mechanical barrier and promote barrier injury repair. The cornu cervi degelatinatum extract has a good application prospect in preparation of drugs for promoting wound healing and products related to strengthening and toughening skin barriers, and resource development and utilization of cornu cervi degelatinatum are enriched.

Stereoselective synthesis of 3,4-diamino-1,2-diols possessing a long hydrocarbon chain was carried out via olefin cross-metathesis between a commercial tetradec-1-ene and known diastereoisomeric trichloroacetamides prepared in our laboratory. Subsequent modification of the obtained coupling products used an approach based on functional group interconversions, which was accompanied by the rational choice of deprotection protocols. The resulting molecules represent novel isomeric amino analogues of natural D-ribo-phytosphingosine, which on the basis of their structure could have the status of skin sphingomimetics, i.e. substances that can affect the properties of the skin and dermis in terms of improving the quality of the lipid matrix (e.g. change in permeability, hydration), or stimulating the biosynthesis of procollagen in deeper structures. The cytotoxic effect of the prepared aminoisophytosphingosines was examined on two human non-malignant cell lines (BJ and HaCaT) and the screening results confirmed the safety in at least three representatives of the prepared series of compounds. In addition, the ability of the synthesized derivatives to inhibit cancer cell proliferation was also investigated. The results of preliminary anticancer screening showed weaker antiproliferative potential of these unusual sphingoid bases.

A perilla leaf-derived nanovesicle and the use thereof in the preparation of a product with anti-inflammatory efficacy. The nanovesicle can be phagocytosed and internalized by HaCaT cells, reduce the levels of ROS and inflammatory factors in HaCaT cells, and exert an anti-inflammatory effect. Meanwhile, the nanovesicle has excellent transdermal properties, and thus can effectively alleviate and treat psoriatic dermatitis symptoms in mice. The nanovesicle can also be added as an active ingredient to different matrices to form compositions.

The invention belongs to the technical field of biological polypeptide research, and particularly relates to a polypeptide capable of removing active oxygen and increasing SIRT gene expression quantity and application of the polypeptide. The general formula of the sequence of the polypeptide is nicotinic acid-C-C-A-r-B-r-B-r-B-r-A-R-G-D-A-NH2, and the general formula of the sequence of the polypeptide is as follows: Wherein G is glycine, R is arginine, D is aspartic acid, and r is arginine; a is one of tryptophan, tyrosine and phenylalanine, B is one of tryptophan, phenylalanine, tyrosine and 3-cyclohexyl alanine, C is one of tryptophan, tyrosine and phenylalanine, A, B and C are different from one another, r is a D configuration, and other amino acids are L configurations. The polypeptide provided by the invention can significantly improve the expression of longevity protein related genes in HaCaT cells, and can effectively remove ROS in the cells, so that the polypeptide has a wide application prospect in the aspects of aging resistance and oxidation resistance.

The invention discloses a preparation method of prune exosomes and application of the prune exosomes in regulation and control of pigment metabolism and skin cell inflammatory response, and the preparation method comprises the following steps: taking prune, washing, peeling, dicing, juicing, adding a compound enzyme preparation, centrifuging at 2,000 g for 20 min, carrying out trehalose-assisted ultracentrifugation, centrifuging at 12,000 g for 30 min, centrifuging at 40,000 g for 60 min, centrifuging at 120,000 g for 70 min, collecting precipitate PBS, re-suspending, and filtering to obtain the high-purity prune exosomes. It is proved for the first time that the prune exosome dose-dependently inhibits the melanin content and tyrosinase activity in B16F10 melanoma cells, the mRNA expression level of key inflammatory factors TNF-alpha, IL-1beta and IL-6 in TNF-alpha-induced Hacat cells is remarkably reduced, in addition, the inhibition effect of melanin can be amplified through an STAT3 channel by combining the prune exosome with luteolin, and the application of the prune exosome to the preparation of the anti-inflammatory drug is promoted. The polypeptide can be used for preparing a preparation for regulating pigment anabolism and skin-related inflammatory response, and a potential biological application value is exerted.

The invention discloses algin with anti-photoaging activity as well as a preparation method and application of the algin, and belongs to the technical field of deep processing of seaweed. The molecular weight of the algin is 10-350kDa, the total sugar content is 40wt%-80wt%, the reducing sugar content is 3wt%-16wt%, and the uronic acid content is 40wt%-80wt%. The preparation method of the algin specifically comprises the following steps: (1) degrading dielectric barrier discharge plasma; and (2) dialyzing and freeze-drying. The algin can significantly improve the inhibition rate of elastase and the cell survival rate of HaCaT cells after ultraviolet irradiation, has significant anti-photoaging activity, and can be used for developing anti-photoaging functional foods and skin care products. Meanwhile, the preparation method adopting the dielectric barrier discharge plasma is simple and convenient to operate, rapid, efficient, green, environment-friendly and free of addition of exogenous chemical substances, and the molecular weight of the algin can be remarkably reduced, so that the additional value of the algin is improved, the application range of the algin is widened, and the algin has a good application prospect.

The invention discloses TCN1 (Trancobalamin 1, cobalamin 1) and application of an inhibitor of the TCN1 in preparation of products for diagnosing and treating diseases of an autoimmune system. According to the invention, expression in psoriasis tissues is up-regulated and promoter DNA methylation is down-regulated into genes to screen TCN1 genes, an over-expression TCN1 HaCaT cell line is constructed, and it is verified that the over-expression TCN1 HaCaT cell line participates in keratinocyte proliferation and IL-17 activation as an autoantigen, can be used as a diagnostic marker and a therapeutic target of autoimmune skin diseases such as psoriasis and the like, and has broad application prospects. And a new direction is provided for mechanism research and treatment of autoimmune skin diseases such as psoriasis.

This invention provides an anti-aging and repair dressing containing four complex active factors and its preparation method. The dressing comprises 75-90 parts of a matrix material, 5-15 parts of four complex active factors, and 3-8 parts of a moisturizing auxiliary agent. The four complex active factors are composed of recombinant human type III collagen, acetyl hexapeptide-8, ectoine, and dipotassium glycyrrhizate in a ratio of (3-6):(1-3):(2-4):(1-2). The matrix is ​​a hydrophilic nonwoven fabric or bio-cellulose membrane material that has undergone low-temperature plasma surface grafting activation treatment. The preparation process employs nitrogen combined with vacuum pulse freeze-drying technology, and enhances the activity stability through an ectoine complexation step. This dressing can promote HaCaT cell proliferation, increase collagen secretion, and inhibit inflammatory factors, significantly improving skin barrier function. It has good biocompatibility and can be used to prepare products that improve skin aging and repair.

Disclosed is use of danshensu or a pharmaceutically acceptable salt thereof as a PI3K / AKT / mTOR signaling pathway inhibitor in the preparation of a drug for treating psoriasis. Pharmacological experiments reveal that danshensu can inhibit the phosphorylation of the PI3K / AKT / mTOR signaling pathway to significantly ameliorate psoriasis-like skin lesions on mice and reduce the excessive proliferation and abnormal differentiation of epidermal cells and the expression of CCL20mRNA in skin lesions. In vitro experiments show that danshensu can inhibit TNF-α-induced HaCaT cell proliferation and inhibit the generation and secretion of the pro-inflammatory factors IL-6, IL-8, and IFN-γ and the chemokine CCL20. The use of danshensu to prepare a drug for treating psoriasis opens up a new field of application for danshensu.

The invention discloses sargassum fusiforme polysaccharide with a barrier repairing function and a preparation method and application thereof. The molecular weight of the sargassum fusiforme polysaccharide is 39-278 kDa, the total sugar content is 50-55 wt%, and the uronic acid content is 29-35 wt%. The preparation method comprises the following steps: (1) hot water extraction: mixing sargassum fusiforme powder with water, and performing hot water extraction, evaporation concentration, alcohol precipitation and freeze-drying; (2) ultrasonic treatment: mixing with water, and performing ultrasonic degradation; and (3) UV / H2O2 degradation: mixing with a H2O2 solution, carrying out UV degradation, removing H2O2, dialyzing, evaporating and concentrating, and freeze-drying to obtain the product. The sargassum fusiforme polysaccharide prepared by the invention can significantly improve the content and expression of filagarisin (FLG) of nude mice and HaCaT cells after UVB irradiation, reduces the percutaneous moisture loss value (TEWL) in nude mice, has a significant skin barrier repair function, and can be used for development and utilization of functional foods and skin care products.

The invention provides a fusion protein with high repair capacity, a preparation method and application thereof, and belongs to the technical field of hybrid peptides. The amino acid sequence of the fusion protein is shown as SEQ ID NO.1; the fusion protein is obtained by sequentially connecting the following modules in series: a functional domain 1 of fibronectin, a hydrophilic region 1 of elastin, a functional domain 1 of mussel mucin, a hydrophilic region 2 of elastin, a functional domain 2 of fibronectin, a hydrophilic region 3 of elastin, a functional domain 2 of mussel mucin, a hydrophilic region 4 of elastin and an integrin binding region of elastin. The fusion protein of fibronectin, elastin and mussel mucin is constructed, and the nucleotide sequence of the fusion protein is optimized, so that the relative migration rate of keratinocytes can be obviously improved, the expression quantity of repair-related genes of HaCat cells can be improved, the secretion quantity of skin stimulating factors can be reduced, and the inhibition rate on hyaluronidase can be improved; the skin repairing and relieving capability is improved.

The invention discloses staphylococcus warneri CCSM005 separated from human skin and capable of improving skin health and a metagen thereof, and belongs to the technical field of microorganisms and medicines. The staphylococcus warneri CCSM005 provided by the invention can be fermented to produce the L-glycine-valine dipeptide, and a fermentation supernatant of the staphylococcus warneri CCSM005 has a good effect of repairing a skin barrier function, which is specifically shown in that the activity of damaged HaCaT cells is improved in vitro, and the gene level expression of FLG, ZO-1, CLDN and Occludin can be improved at the same time after the damage. Therefore, the staphylococcus warneri CCSM005 has a huge application prospect in preparation of external daily chemical products or medicines for repairing the skin barrier.

The invention relates to the technical field of skin care products, in particular to a turnip extracting solution and a preparation method and application thereof. The preparation method comprises the following steps: mixing turnip with water, decocting to obtain a decoction, decolorizing, filtering and concentrating to obtain the turnip extracting solution. The turnip extracting solution is used for effectively inhibiting B16 cells from generating melanin and inhibiting the activity of tyrosinase in a melanin synthesis path; the increase of the active oxygen level in HaCaT cells is effectively prevented, and the antioxidant effect is obvious; the inflammation of the SDS surfactant on HaCaT cells is effectively prevented, and the generation of an inflammatory factor TNF-alpha is reduced; the expression of cell natural moisturizing factors, ceramide de novo synthesis rate-limiting enzymes and epidermal barrier related genes is up-regulated; the composition can effectively promote the generation of cell collagen and elastin, reduces the synthesis of matrix metalloproteinase 1 for degrading collagen under ultraviolet radiation, and has an anti-aging effect.

The invention discloses an active polypeptide for promoting healing of oral ulcer and application thereof, and belongs to the technical field of biological medicines. The amino acid sequence of the active polypeptide NPTX3 capable of promoting healing of the oral ulcer is DLKPIAEDIPSLEK. An in-vitro experiment result shows that the polypeptide NPTX3 can remarkably promote the scratch closure of the human immortalized keratinocytes at the concentration as low as 500 pM. Transwell migration experiments further prove that the polypeptide NPTX3 can obviously enhance the migration ability of HaCaT cells at the concentrations of 1nM and 10nM, which prompts that the polypeptide NPTX3 may accelerate wound repair by promoting epithelial cell migration. In an SD (Sprague Dawley) rat oral ulcer model, the ulcer healing rate can be obviously improved by locally applying the polypeptide NPTX3 (1 nM, 10 nM). The polypeptide NPTX3 provided by the invention shows excellent activity of promoting cell migration and tissue repair at an extremely low dose, has the advantages of low effective dose, strong biological activity, high safety and the like, and provides an efficient and mild new choice for drug treatment of oral ulcer.

The present application provides a kind of oil composition, containing monounsaturated fatty acid, polyunsaturated fatty acid, monounsaturated fatty acid is omega-9 fatty acid;Polyunsaturated acid is the mixture of omega-6 fatty acid and omega-3 fatty acid.The present application can repair and resist aging, all-purpose skin care, increase epidermal thickness, enhance barrier, anti-inflammatory soothing, anti-wrinkle firming, soothing repair red, nourish skin, and can be applied to sensitive skin, can inhibit LPS-induced HaCaT cell IL-6 secretion, increase the thickness of 3D skin model epidermis layer, and can promote the expression of silk aggregate protein (FLG), ceramide synthase 3 (CerS3), desmoglein core glycoprotein (DSG1) which has important role for skin barrier function, indicating that it can alleviate the inflammatory response produced by LPS stimulation HaCaT cell and has enhanced skin barrier function.

The invention discloses an algae protein peptide with anti-radiation activity and a preparation method and application thereof. The method comprises the following steps: adding pectinase and cellulase into a red algae raw material, and carrying out sol dissolving and cell dissolving to release red algae protein; the preparation method comprises the following steps: by taking a red algae protein peptide as a raw material, cutting a subunit by virtue of enzymatic conversion to identify a disulfide bond and a peptide bond, so as to obtain the red algae protein peptide with a specific functional fragment component, and directionally preparing the algae protein peptide with an anti-radiation function through activity verification, so that the prepared algae protein peptide has an extremely strong protection effect on ultraviolet irradiation HaCat cells, can effectively relieve cell DNA damage caused by UVB, and can be used for preparing the anti-radiation algae protein peptide. The cell survival rate of UVB irradiation is improved, and the death rate is reduced, so that the algae protein peptide can be applied to preparation of products such as food, cosmetics and drinks, and has a good anti-radiation effect.

The invention provides a screening method and application of external traditional Chinese medicine anti-psoriasis active ingredients, and relates to the technical field of modern research of traditional Chinese medicines. According to the screening method disclosed by the invention, components with transdermal ability and anti-psoriasis activity are systematically excavated and screened from a traditional Chinese medicine complex system through preparation of a traditional Chinese medicine transdermal sample solution, UPLC-Q-TOF-MS component identification, network pharmacological analysis, molecular docking prediction and HaCaT psoriasis cell model verification in sequence. The method solves the problems that the traditional Chinese medicine transdermal components are unclear in pharmacodynamic material basis and low in screening efficiency, and has the advantages of being high in systematicness, high in accuracy and good in pertinence. According to the method, a plurality of effective components such as dictamnine and limonin are successfully screened from the cortex dictamni, and a clear pharmacological basis is provided for research and development of the cortex dictamni externally applied anti-psoriasis medicine.

The invention discloses a hyssopus officinalis extract and a preparation method and application thereof.The preparation method of the hyssopus officinalis extract comprises the following steps that a mixed solution of hyssopus officinalis and honey is covered, moistened and stir-fried, and a primary product is obtained; and heating a mixed solution of the head product and polyhydric alcohol at the temperature of 80-100 DEG C for 5-10 hours to prepare the hyssopus officinalis extract. The hyssopus officinalis extract prepared by the preparation method disclosed by the invention can activate endogenous antioxidation of a human body, inhibit expression of MMP-1, promote synthesis of elastin in human true epidermis cells, promote expression of HSP27 in HaCaT cells and down-regulate expression of HMGB1 in the HaCaT cells, and has a better anti-photoaging effect.

The invention provides a ferulate compound as well as a preparation method and application thereof, the ferulate compound is (5-formyl-2-furan) methyl dihydroferulate, the ferulate compound is prepared by the following steps: extracting a dry whole herb water extract of eyebright by using ethyl acetate, recovering a solvent to obtain an ethyl acetate extraction part, and separating by using normal-phase silica gel column chromatography to obtain the (5-formyl-2-furan) methyl dihydroferulate. Petroleum ether-ethyl acetate is used as an eluent for gradient elution, a fraction Fr.2 is obtained and separated by normal-phase silica gel column chromatography to obtain a fraction Fr.2. 2, Sephadex LH-20 gel column chromatography is performed to obtain a fraction Fr.2. 2.1, MC I column chromatography is performed to separate the fraction Fr.2. 2.1, methanol-water is used as an eluent for gradient elution to obtain a fraction Fr.2. 1.1, and then preparative HPLC (High Performance Liquid Chromatography) is performed to obtain the HaCaT cell activity. The composition has a protection effect on HaCaT cell oxidative damage caused by UVB, and can effectively resist skin photoaging.