82 results about "HaCaT" patented technology

The invention belongs to the technical field of microorganisms, and particularly relates to an extracellular vesicle of phytobacterium plantarum and application of the extracellular vesicle. Specifically, the plant lactobacillus extracellular vesicles can promote HaCaT cells to generate ceramide, up-regulate expression of key genes in a skin ceramide synthesis route and recover a skin barrier; the expression of proinflammatory factors in a cell inflammation model can be reduced, and a relatively good anti-inflammatory effect is achieved. A mouse skin injury model verifies that the EVs has good skin infiltration capacity and can remarkably reduce the skin injury score, relieve skin itch, improve the skin water content and reduce expression of proinflammatory factors in skin tissue and expression of pruritus-related cell factors.

The invention relates to a sodium hyaluronate fermentation filtrate as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. Through a two-stage fermentation mode, the fermentation filtrate containing sodium hyaluronate is prepared by recombining bacillus subtilis, and then residual nutrient substances in a culture medium are consumed by inoculating bacillus velezensis, so that the nutrient substances are metabolized into active substances with repairing and strengthening effects on hair; the prepared sodium hyaluronate fermentation filtrate has the effects of repairing scalp and strengthening hair. The sodium hyaluronate filtrate disclosed by the invention can be used for well reducing the content of NO in macrophages (the NO content of cells is increased due to malassezia lysate), can be used for removing active oxygen ROS of HaCaT cells and increasing the total tensile strength of hair, and has the potential effects of relieving scalp, resisting oxidative stress and enhancing the toughness of the hair.

The invention discloses a strain of Blastococcus atamensis YC-1 and application of the strain of Blastococcus atamensis YC-1, and belongs to the field of microbial strains. The streptococcus sp. YC-1 provided by the invention has high antioxidant activity and also has the capability of producing DHHB (docosahexaenoic acid); meanwhile, the YC-1FLE is prepared by adopting absolute ethyl alcohol as a solvent, has no toxicity to HaCaT cells, is relatively high in reducing capacity, has a scavenging effect on DPPH free radicals, ABTS cation free radicals and hydroxyl free radicals, has a tyrosinase inhibition effect and shows good antioxidant and whitening effects; when the YC-1FLE is applied to the sunscreen lotion, the sunscreen coefficient of the sunscreen lotion containing the YC-1FLE is obviously higher than that of a blank control group, which shows that the YC-1FLE has better ultraviolet resistance. Therefore, the strain and the lysate extract of the fermentation product of the strain have huge application potential in the field of cosmetics.

The invention discloses a plant lactobacillus with light aging resistance and an application thereof. The preservation number of the plant lactobacillus cj001 disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No.35419. The fermentation supernatant of the plant lactobacillus cj001 shows good anti-aging activity and in-vitro antioxidant activity, and the growth ability of HaCaT cells is promoted. After the combined fermentation product of the plant lactobacillus cj001 and the lactobacillus paracasei Grx701 is subjected to ultraviolet radiation, the proliferation of HaCaT cells can be promoted, the endogenous antioxidant capacity of human skin cells can be enhanced, the photooxidation damage can be effectively relieved, and the combined fermentation product has a wide application value in the field of preparation of products with antioxidant, anti-aging, anti-ultraviolet damage, anti-photoaging and the like.

A perilla leaf-derived nanovesicle and the use thereof in the preparation of a product with anti-inflammatory efficacy. The nanovesicle can be phagocytosed and internalized by HaCaT cells, reduce the levels of ROS and inflammatory factors in HaCaT cells, and exert an anti-inflammatory effect. Meanwhile, the nanovesicle has excellent transdermal properties, and thus can effectively alleviate and treat psoriatic dermatitis symptoms in mice. The nanovesicle can also be added as an active ingredient to different matrices to form compositions.

The invention discloses a preparation method of prune exosomes and application of the prune exosomes in regulation and control of pigment metabolism and skin cell inflammatory response, and the preparation method comprises the following steps: taking prune, washing, peeling, dicing, juicing, adding a compound enzyme preparation, centrifuging at 2,000 g for 20 min, carrying out trehalose-assisted ultracentrifugation, centrifuging at 12,000 g for 30 min, centrifuging at 40,000 g for 60 min, centrifuging at 120,000 g for 70 min, collecting precipitate PBS, re-suspending, and filtering to obtain the high-purity prune exosomes. It is proved for the first time that the prune exosome dose-dependently inhibits the melanin content and tyrosinase activity in B16F10 melanoma cells, the mRNA expression level of key inflammatory factors TNF-alpha, IL-1beta and IL-6 in TNF-alpha-induced Hacat cells is remarkably reduced, in addition, the inhibition effect of melanin can be amplified through an STAT3 channel by combining the prune exosome with luteolin, and the application of the prune exosome to the preparation of the anti-inflammatory drug is promoted. The polypeptide can be used for preparing a preparation for regulating pigment anabolism and skin-related inflammatory response, and a potential biological application value is exerted.

The invention discloses TCN1 (Trancobalamin 1, cobalamin 1) and application of an inhibitor of the TCN1 in preparation of products for diagnosing and treating diseases of an autoimmune system. According to the invention, expression in psoriasis tissues is up-regulated and promoter DNA methylation is down-regulated into genes to screen TCN1 genes, an over-expression TCN1 HaCaT cell line is constructed, and it is verified that the over-expression TCN1 HaCaT cell line participates in keratinocyte proliferation and IL-17 activation as an autoantigen, can be used as a diagnostic marker and a therapeutic target of autoimmune skin diseases such as psoriasis and the like, and has broad application prospects. And a new direction is provided for mechanism research and treatment of autoimmune skin diseases such as psoriasis.

This invention provides an anti-aging and repair dressing containing four complex active factors and its preparation method. The dressing comprises 75-90 parts of a matrix material, 5-15 parts of four complex active factors, and 3-8 parts of a moisturizing auxiliary agent. The four complex active factors are composed of recombinant human type III collagen, acetyl hexapeptide-8, ectoine, and dipotassium glycyrrhizate in a ratio of (3-6):(1-3):(2-4):(1-2). The matrix is ​​a hydrophilic nonwoven fabric or bio-cellulose membrane material that has undergone low-temperature plasma surface grafting activation treatment. The preparation process employs nitrogen combined with vacuum pulse freeze-drying technology, and enhances the activity stability through an ectoine complexation step. This dressing can promote HaCaT cell proliferation, increase collagen secretion, and inhibit inflammatory factors, significantly improving skin barrier function. It has good biocompatibility and can be used to prepare products that improve skin aging and repair.

Disclosed is use of danshensu or a pharmaceutically acceptable salt thereof as a PI3K / AKT / mTOR signaling pathway inhibitor in the preparation of a drug for treating psoriasis. Pharmacological experiments reveal that danshensu can inhibit the phosphorylation of the PI3K / AKT / mTOR signaling pathway to significantly ameliorate psoriasis-like skin lesions on mice and reduce the excessive proliferation and abnormal differentiation of epidermal cells and the expression of CCL20mRNA in skin lesions. In vitro experiments show that danshensu can inhibit TNF-α-induced HaCaT cell proliferation and inhibit the generation and secretion of the pro-inflammatory factors IL-6, IL-8, and IFN-γ and the chemokine CCL20. The use of danshensu to prepare a drug for treating psoriasis opens up a new field of application for danshensu.

The invention discloses staphylococcus warneri CCSM005 separated from human skin and capable of improving skin health and a metagen thereof, and belongs to the technical field of microorganisms and medicines. The staphylococcus warneri CCSM005 provided by the invention can be fermented to produce the L-glycine-valine dipeptide, and a fermentation supernatant of the staphylococcus warneri CCSM005 has a good effect of repairing a skin barrier function, which is specifically shown in that the activity of damaged HaCaT cells is improved in vitro, and the gene level expression of FLG, ZO-1, CLDN and Occludin can be improved at the same time after the damage. Therefore, the staphylococcus warneri CCSM005 has a huge application prospect in preparation of external daily chemical products or medicines for repairing the skin barrier.

The invention discloses an active polypeptide for promoting healing of oral ulcer and application thereof, and belongs to the technical field of biological medicines. The amino acid sequence of the active polypeptide NPTX3 capable of promoting healing of the oral ulcer is DLKPIAEDIPSLEK. An in-vitro experiment result shows that the polypeptide NPTX3 can remarkably promote the scratch closure of the human immortalized keratinocytes at the concentration as low as 500 pM. Transwell migration experiments further prove that the polypeptide NPTX3 can obviously enhance the migration ability of HaCaT cells at the concentrations of 1nM and 10nM, which prompts that the polypeptide NPTX3 may accelerate wound repair by promoting epithelial cell migration. In an SD (Sprague Dawley) rat oral ulcer model, the ulcer healing rate can be obviously improved by locally applying the polypeptide NPTX3 (1 nM, 10 nM). The polypeptide NPTX3 provided by the invention shows excellent activity of promoting cell migration and tissue repair at an extremely low dose, has the advantages of low effective dose, strong biological activity, high safety and the like, and provides an efficient and mild new choice for drug treatment of oral ulcer.

The present application provides a kind of oil composition, containing monounsaturated fatty acid, polyunsaturated fatty acid, monounsaturated fatty acid is omega-9 fatty acid;Polyunsaturated acid is the mixture of omega-6 fatty acid and omega-3 fatty acid.The present application can repair and resist aging, all-purpose skin care, increase epidermal thickness, enhance barrier, anti-inflammatory soothing, anti-wrinkle firming, soothing repair red, nourish skin, and can be applied to sensitive skin, can inhibit LPS-induced HaCaT cell IL-6 secretion, increase the thickness of 3D skin model epidermis layer, and can promote the expression of silk aggregate protein (FLG), ceramide synthase 3 (CerS3), desmoglein core glycoprotein (DSG1) which has important role for skin barrier function, indicating that it can alleviate the inflammatory response produced by LPS stimulation HaCaT cell and has enhanced skin barrier function.

The invention provides a screening method and application of external traditional Chinese medicine anti-psoriasis active ingredients, and relates to the technical field of modern research of traditional Chinese medicines. According to the screening method disclosed by the invention, components with transdermal ability and anti-psoriasis activity are systematically excavated and screened from a traditional Chinese medicine complex system through preparation of a traditional Chinese medicine transdermal sample solution, UPLC-Q-TOF-MS component identification, network pharmacological analysis, molecular docking prediction and HaCaT psoriasis cell model verification in sequence. The method solves the problems that the traditional Chinese medicine transdermal components are unclear in pharmacodynamic material basis and low in screening efficiency, and has the advantages of being high in systematicness, high in accuracy and good in pertinence. According to the method, a plurality of effective components such as dictamnine and limonin are successfully screened from the cortex dictamni, and a clear pharmacological basis is provided for research and development of the cortex dictamni externally applied anti-psoriasis medicine.

The invention discloses a screening method for verifying photoaging resistance effectiveness of bioactive components. Comprising the following steps: by adopting a H2O2 damage model of HaCaT cells, detecting the cell migration rate of active ingredients, and / or detecting the expression level of senescence-related genes p21, p16 and p53, and / or detecting the expression level of senescence-related protein SIRT1; the photoaging resistance effectiveness of the active ingredients is further evaluated by detecting the expression and positioning of autophagy related proteins LC3 and p62, and compared with a traditional model and method, the photoaging resistance effectiveness of the active ingredients is more stable, accurate and efficient.

The invention discloses a metagen which is prepared from bifidobacterium longum CCFM1508 and has the effects of regulating skin ceramide de novo synthesis and improving skin health, and belongs to the technical field of microorganisms and medicines. The bifidobacterium longum CCFM1508 and the metagen prepared from the bifidobacterium longum CCFM1508, provided by the invention, have the effects of promoting ceramide synthesis and improving skin health by external use and oral administration, and are specifically shown as follows: a thallus lysate and a fermented supernatant of the bifidobacterium longum CCFM1508 can promote HaCaT cells to secrete ceramide in the aspect of external use, and the thallus lysate can remarkably improve the activity of the HaCaT cells. And the viable bacteria and the postbiotics of the bifidobacterium longum CCFM1508 which are orally taken have obvious effects in skin apparent indexes, the content of cerayl and proinflammatory factors. Therefore, the bifidobacterium longum has huge application prospects in preparation of external or oral medicines for promoting synthesis of skin ceramide and improving skin health and preparation of food, health care products or cosmetics.

An induction medium and induction culture method for lavender suspension cells having high antioxidant activity. Suspension cells having a high ABTS free radical scavenging rate can be cultured by optimizing the formulation of a medium, and the contents of total phenols, total flavonoids and total triterpenoids are relatively high. Additionally, lavender suspension cells induced by using the medium grow rapidly and have a stable system, and extracts thereof can significantly reduce redundant ROS generated by oxidative stress of HaCaT cells, exhibit good biological antioxidant activity, and provide a reference for the development of lavender secondary metabolite resources and the like.

The application discloses a new use of androsta-4,6,8(9),13(14)-tetraen-3,11,16-trione, namely, application of the androsta-4,6,8(9),13(14)-tetraen-3,11,16-trione in preparation of a medicine for treating lymphoma. 50 The IC value of the androsta-4,6,8(9),13(14)-tetraen-3,11,16-trione in the SR cell line is detected by a CCK-8 method, and the cytotoxicity of the androsta-4,6,8(9),13(14)-tetraen-3,11,16-trione on human umbilical vein endothelial cells Huvce and human immortalized keratinocytes Hacat is detected, in the body, the androsta-4,6,8(9),13(14)-tetraen-3,11,16-trione significantly inhibits tumor growth and shows good biological safety and no systemic toxicity; TUNEL staining, gamma-H2AX staining and neutral comet experiment prove that the compound of the application inhibits the growth of SR by causing serious damage to DNA, the application not only adds the diversity of the biological activity of the androsta-4,6,8(9),13(14)-tetraen-3,11,16-trione, but also provides a new direction for developing a new medicine for treating lymphoma.

This invention discloses a 2-hydroxynicotinic acid-2-hydroxybenzyl ester, its preparation method, and its application. The structural formula of the 2-hydroxynicotinic acid-2-hydroxybenzyl ester is shown below. This invention utilizes 2-hydroxynicotinic acid as a raw material and obtains a 2-hydroxynicotinic acid-2-hydroxybenzyl ester with a novel structure through esterification. The 2-hydroxynicotinic acid-2-hydroxybenzyl ester has low cytotoxicity, can scavenge reactive oxygen species, and has good anti-UVB-induced oxidative damage activity in HaCaT cells. It can be used in products for anti-skin oxidative damage.

The invention discloses a method for preparing rare saponin based on compound lactobacillus fermentation of black ginseng, a product, a preparation and application, and belongs to the technical field of traditional Chinese medicinal material fermentation. The technical problem to be solved is to provide the preparation method of the rare saponin (Rg3, Rg5, Rk1 and Rh4) compound, the content of the rare saponin is obviously increased after compound lactic acid bacteria treatment, the SOD activity of UVB-induced HaCat cells is obviously improved, the MDA content is obviously reduced, and the melanin content and tyrosinase activity in alpha-MSH-induced B16 cells and zebra fish are reduced. The key point of the technical scheme is as follows: the compound lactic acid bacteria consist of lactobacillus plantarum ACCC11095, lactobacillus casei CGMCC 1.29, lactobacillus rhamnosus ATCC7596 and lactobacillus acidophilus ATCC4356.

The invention discloses a bone-derived collagen peptide with a joint protection effect and a preparation method thereof, and belongs to the technical field of collagen peptide preparation, the preparation method comprises the following steps: S1, cleaning and crushing fresh fish bones, and performing impurity protein removal treatment on fish bone powder by using a 0.1 M NaOH solution to obtain pretreated bone powder; s2, mixing the pretreated bone meal with 0.5-1.5 mol / L hydrochloric acid according to a solid-to-liquid ratio of 1: 5, and carrying out a decalcification reaction for 2-4 hours to obtain decalcified bone collagen; s3, performing sequential enzymolysis on the decalcified ossein by adopting pepsin and trypsin to obtain enzymatic hydrolysate; and carrying out inactivation, purification, vacuum concentration and freeze drying on the enzymatic hydrolysate to obtain the collagen peptide. According to the collagen peptide and the preparation method, the characteristic amino acid content of the prepared collagen peptide is increased by 40% or above compared with that of a traditional process, it is ensured that the collagen peptide is combined with a cartilage cell receptor in a targeted mode and inhibits secretion of inflammatory factors, and the inhibition rate reaches 58-68%; at a concentration of 500 [mu] g / mL, there is no toxicity to HaCaT cells (IC50gt; and the traditional Chinese medicine composition has no toxic or side effect and no skin irritation.

The invention provides champignon luteo-virens flavone, an extraction method thereof, a whitening and skin care composition and a mask, and belongs to the technical field of cosmetics. The method for extracting the flavonoids of the desmodium luteo-virens comprises the following steps: (1) preparing the desmodium luteo-virens into powder, and carrying out enzymolysis pretreatment to obtain enzymatic hydrolysate; (2) adding an ethanol solution into the enzymatic hydrolysate, carrying out ultrasonic extraction, and collecting an extracting solution; and (3) purifying the extracting solution by adopting macroporous resin to obtain a champignon luteo-virens flavone purification product. According to the method disclosed by the invention, the flavone component is successfully extracted from the fruiting body of the Litsea luteo-virens, and the Litsea luteo-virens flavone product with the purity of 88.6% is obtained through macroporous resin purification. Experimental groups prove that the Lentinus luteo-virens flavone has higher antioxidant activity, has the capability of inhibiting tyrosinase activity, has better antioxidant and anti-inflammatory effects on HaCaT cells, and can be used for preparing cosmetics with antioxidant and anti-inflammatory effects.

The invention discloses sphingomonas CCSM001 separated from human skin and capable of improving skin health and a metagen thereof, and belongs to the field of microbial technology and medicine. The sphingomonas CCSM001 provided by the invention has a good barrier function repairing effect and application prospect in the aspect of external use, and is specifically shown in that the activity of damaged HaCaT cells is improved in vitro, the gene level expression of FLG, IVL, AQP3, ZO-1, Occludin and CLDN is improved in the aspects of functional protein and structural protein after damage. The metagen prepared from the sphingomonas can be used for preparing cosmetics or medicines for repairing skin barriers, and has a huge application prospect.

The application discloses a tripeptide with the effects of promoting HaCaT cell migration and barrier repair and application of the tripeptide, and the amino acid sequence of the tripeptide is glycine-methionine-hydroxyproline. The tripeptide has the effects of promoting HaCaT cell migration and barrier repair, and can be applied to the preparation of cosmetics with the effects of skin tissue repair and / or skin barrier repair.

This invention belongs to the field of psoriasis diagnosis and treatment technology, specifically involving the application of circNCAPH and diagnostic / therapeutic agents for psoriasis. Abnormally high expression of circNCAPH in psoriatic lesions was detected by qRT-PCR, suggesting that circNCAPH holds promise as a diagnostic marker for psoriasis. Due to the excellent silencing effect of siRNA, after ensuring that circNCAPH was suppressed, CCK8 and apoptosis experiments were performed on HaCaT keratinocyte cell lines with silenced circNCAPH. Compared to the NC group, the proliferation rate of cells in the siRNA group was significantly slower, and the proportion of late-stage apoptotic cells was significantly increased, indicating that silencing circNCAPH inhibited keratinocyte proliferation. Therefore, inhibiting circNCAPH can treat psoriasis, which has profound clinical significance and important prospects for widespread application.

The invention relates to application of pholidota chinensis exosome-like nano-vesicles in preparation of a product with a skin care function, and belongs to the technical field of plant biology. The pholidota chinensis exosome-like nano-vesicle is rich in bioactive substances, has excellent biocompatibility and transmembrane delivery potential due to the bimolecular phospholipid layer structure, and has various skin care effects. Tests show that the compound has no toxicity to RBL-2H3 and RAW264.7 cells within the concentration of 6.25-100 [mu] g / mL, has remarkable proliferation promoting capacity to HaCaT cells within the concentration of 50-100 [mu] g / mL, and meets the safety requirements of cosmetics. In addition, the vesicles can effectively remove ABTS < + > and DPPH free radicals, the inhibiting capacity of the vesicles on DPPH is remarkably higher than that of aqueous extracts and close to that of VC, and the vesicles show an excellent antioxidant effect. Meanwhile, NO generation can be remarkably inhibited, and the effect of resisting skin inflammation is achieved.

The invention provides activity evaluation and application of recombinant humanized III-type collagen, which comprises the following steps of: carrying out cell proliferation, cell adhesion and cell migration experiments on the recombinant humanized III-type collagen by adopting a plurality of cell models, namely human skin fibroblasts HSF, cuticle cells HaCat and mouse fibroblasts 3T3; the bioactivity of the recombinant humanized III-type collagen is subjected to comprehensive and deep analysis from multiple dimensions, so that multi-cell and multi-aspect accurate evaluation is realized, and the biological characteristics and functional expressions of the collagen in different cell environments are accurately captured. Experimental data can provide a powerful experimental basis for the application of the recombinant humanized III-type collagen in the fields of cosmetics, medical instruments and the like, so that the potentials of the recombinant humanized III-type collagen in the aspects of cosmetic effects of improving skin appearance, promoting skin repair and the like are clear; and a key reference can be provided for the properties such as tissue repair and cell adhesion involved in the development of medical apparatuses and instruments.

The invention discloses staphylococcus epidermidis CCSM009 for repairing a skin barrier and a metagen of the staphylococcus epidermidis CCSM009, and belongs to the field of microbial technologies and medicines. The staphylococcus epidermidis CCSM009 and the metagen thereof provided by the invention have a good barrier function repairing effect and application prospect in the aspect of external use, and are specifically shown as follows: the activity of damaged HaCaT cells is improved in vitro, and the expression of gene levels and protein levels in the aspects of functional proteins and structural proteins after damage is improved; meanwhile, sphingomyelinase 1 and tissue kininolase 7 (KLK7) are improved, and it is clear that the bacterium performs up-regulation of skin barrier related proteins and enzymes through an AHR-OVOL1 pathway. Therefore, the metagen of the staphylococcus epidermidis CCSM009 has a huge application prospect in preparation of cosmetics or medicines for repairing the skin barrier.

The invention discloses a skin-sourced streptococcus infantis CCSM002 capable of improving skin health and a metagen of the streptococcus infantis CCSM002, and belongs to the field of microbial technologies and medicines. The streptococcus infantis CCSM002 provided by the invention has the capability of generating oleamide, D-erythrodisphingosine and (2-oxo-2, 3-dihydro-1H-indole-3-yl)-acetic acid, and the strain has a good effect of repairing a barrier function in the aspect of external use and a good application prospect, specifically, the activity of damaged HaCaT cells is improved in vitro, the activity of the damaged HaCaT cells is improved, the activity of the damaged HaCaT cells is improved, and the activity of the damaged HaCaT cells is improved. The expression of tissue kininolase is reduced, and the gene level expression of FLG, IVL, AQP3, ZO-1 and Occludin can be improved at the same time in the aspect of improving functional protein and structural protein after damage. Therefore, the streptococcus infantis CCSM002 has a huge application prospect in preparation of external daily chemical products or medicines for repairing the skin barrier.

The invention belongs to the technical field of biology, and discloses a composition for promoting proliferation of human immortalized keratinocytes and application of the composition. The inventor finds that the collagen, the fibronectin, the allantoin and the kava pepper leaf / root / stem extract have a good synergistic effect, proliferation of HaCaT cells can be remarkably promoted, and the composition can accelerate repair of damaged skin barriers. Therefore, the invention has a good application prospect in skin care products.

The invention discloses lactobacillus reuteri CCFM1502 capable of up-regulating skin ceramide synthesis and reducing skin inflammation and a metagen of the lactobacillus reuteri CCFM1502, and belongs to the technical field of microorganisms and medicines. The lactobacillus reuteri CCFM1502 provided by the invention can remarkably promote synthesis of ceramide when being externally used and orally taken, and can reduce skin inflammation response of mice after being orally taken. Specifically, the strain lysate and the fermentation supernatant both can promote HaCaT cells to secrete ceramide; the total amount of ceramide in skin can be up-regulated by oral administration of viable bacteria and metabiotics of the lactobacillus reuteri, apparent indexes of the skin can be regulated and controlled, inflammatory reactions in vivo and tissues can be reduced, and particularly, the effect of fermentation supernate of the lactobacillus reuteri is optimal. And the thallus components and viable bacteria have obvious effects in promoting the expression of anti-inflammatory factors in skin tissues. Therefore, the lactobacillus reuteri has a huge application prospect in preparation of a product for promoting synthesis of the natural active ingredient ceramide of the skin.