14 results about "HaCaT" patented technology

This invention provides an anti-aging and repair dressing containing four complex active factors and its preparation method. The dressing comprises 75-90 parts of a matrix material, 5-15 parts of four complex active factors, and 3-8 parts of a moisturizing auxiliary agent. The four complex active factors are composed of recombinant human type III collagen, acetyl hexapeptide-8, ectoine, and dipotassium glycyrrhizate in a ratio of (3-6):(1-3):(2-4):(1-2). The matrix is ​​a hydrophilic nonwoven fabric or bio-cellulose membrane material that has undergone low-temperature plasma surface grafting activation treatment. The preparation process employs nitrogen combined with vacuum pulse freeze-drying technology, and enhances the activity stability through an ectoine complexation step. This dressing can promote HaCaT cell proliferation, increase collagen secretion, and inhibit inflammatory factors, significantly improving skin barrier function. It has good biocompatibility and can be used to prepare products that improve skin aging and repair.

The present application provides a kind of oil composition, containing monounsaturated fatty acid, polyunsaturated fatty acid, monounsaturated fatty acid is omega-9 fatty acid;Polyunsaturated acid is the mixture of omega-6 fatty acid and omega-3 fatty acid.The present application can repair and resist aging, all-purpose skin care, increase epidermal thickness, enhance barrier, anti-inflammatory soothing, anti-wrinkle firming, soothing repair red, nourish skin, and can be applied to sensitive skin, can inhibit LPS-induced HaCaT cell IL-6 secretion, increase the thickness of 3D skin model epidermis layer, and can promote the expression of silk aggregate protein (FLG), ceramide synthase 3 (CerS3), desmoglein core glycoprotein (DSG1) which has important role for skin barrier function, indicating that it can alleviate the inflammatory response produced by LPS stimulation HaCaT cell and has enhanced skin barrier function.

The application discloses a new use of androsta-4,6,8(9),13(14)-tetraen-3,11,16-trione, namely, application of the androsta-4,6,8(9),13(14)-tetraen-3,11,16-trione in preparation of a medicine for treating lymphoma. 50 The IC value of the androsta-4,6,8(9),13(14)-tetraen-3,11,16-trione in the SR cell line is detected by a CCK-8 method, and the cytotoxicity of the androsta-4,6,8(9),13(14)-tetraen-3,11,16-trione on human umbilical vein endothelial cells Huvce and human immortalized keratinocytes Hacat is detected, in the body, the androsta-4,6,8(9),13(14)-tetraen-3,11,16-trione significantly inhibits tumor growth and shows good biological safety and no systemic toxicity; TUNEL staining, gamma-H2AX staining and neutral comet experiment prove that the compound of the application inhibits the growth of SR by causing serious damage to DNA, the application not only adds the diversity of the biological activity of the androsta-4,6,8(9),13(14)-tetraen-3,11,16-trione, but also provides a new direction for developing a new medicine for treating lymphoma.

This invention discloses a 2-hydroxynicotinic acid-2-hydroxybenzyl ester, its preparation method, and its application. The structural formula of the 2-hydroxynicotinic acid-2-hydroxybenzyl ester is shown below. This invention utilizes 2-hydroxynicotinic acid as a raw material and obtains a 2-hydroxynicotinic acid-2-hydroxybenzyl ester with a novel structure through esterification. The 2-hydroxynicotinic acid-2-hydroxybenzyl ester has low cytotoxicity, can scavenge reactive oxygen species, and has good anti-UVB-induced oxidative damage activity in HaCaT cells. It can be used in products for anti-skin oxidative damage.

The application discloses a tripeptide with the effects of promoting HaCaT cell migration and barrier repair and application of the tripeptide, and the amino acid sequence of the tripeptide is glycine-methionine-hydroxyproline. The tripeptide has the effects of promoting HaCaT cell migration and barrier repair, and can be applied to the preparation of cosmetics with the effects of skin tissue repair and / or skin barrier repair.

The present application relates to the technical field of plant extract, and discloses a preparation method of Hericium erinaceus extract and application of the Hericium erinaceus extract in cosmetics, which comprises the following steps: taking Hericium erinaceus raw materials, adopting ethanol reflux extraction, combining the extraction liquid, and obtaining extract by vacuum concentration; liquid-liquid extraction is carried out by using ethyl acetate, and the Hericium erinaceus extract is obtained by concentration and freeze-drying. The main active ingredient of Hericium erinaceus extract is Hericenones C by HPLC separation. The activity verification experiment shows that the extract has significant antioxidant activity, the IC50 value of DPPH free radical scavenging capacity is 239±7 μg / mL; the IC50 value of hydroxyl radical (·OH) scavenging capacity is 547±15 μg / mL; the Trolox equivalent antioxidant capacity (TEAC) of ABTS+· free radical scavenging capacity is 477.3±3.1 μmol TE / g; the extract has significant proliferation promoting effect on HaCaT cells, and the maximum proliferation rate is 146.44±1.27%. The present application solves the technical problems that the components of Hericium erinaceus extract are not clear and the UVB resistance activity is not developed, and provides a technical basis for the application of the extract in cosmetics as anti-wrinkle, repair and anti-aging raw materials.

This invention discloses a wolfberry leaf extract and its application in the preparation of acne prevention and treatment products. The invention uses specific process steps to prepare different solvent extracts of wolfberry leaves. This wolfberry leaf extract can reduce the level of lipopolysaccharide-induced inflammatory factors in Hacat cells and significantly improve acne symptoms in a rat acne lesion model. The wolfberry leaf extract provided by this invention has the potential to become a therapeutic product for acne and exhibits good safety.

The application discloses an algal protein peptide with anti-radiation activity and a preparation method and application thereof. The algal protein peptide is prepared by the following steps: adding pectinase and cellulase into red alga raw materials to release the algal protein by sol and lysis; adopting enzyme conversion to cut subunits to identify disulfide bonds and peptide bonds, so that a specific functional fragment component of the algal protein peptide is obtained; and through activity verification, the algal protein peptide with anti-radiation function is prepared in a targeted manner. The prepared algal protein peptide has a very strong protective effect on ultraviolet radiation HaCat cells, can effectively relieve cell DNA damage caused by UVB, improve cell survival rate under UVB irradiation, and reduce mortality. Therefore, the algal protein peptide can be applied to the preparation of food, cosmetics, drinks and other products, and has a good anti-radiation effect.

This invention discloses a polypeptide and its applications, belonging to the field of biomedical technology. The amino acid sequence of the polypeptide is shown in SEQ ID NO: 1. The polypeptide is prepared by conventional chemical solid-phase synthesis or recombinant expression. In vitro, the polypeptide significantly inhibits the inflammatory response of HaCaT cells induced by M5, exhibiting good anti-inflammatory activity. In vivo experiments show that the polypeptide can effectively improve imiquimod-induced psoriasis-like symptoms in mice. The degree of epidermal acanthosis was significantly reduced in the polypeptide-treated group, and the number and infiltration of T cells in the lesion site were significantly decreased, indicating that the polypeptide can improve the histopathological characteristics of psoriasis by inhibiting epidermal acanthosis and reducing T cell infiltration. Furthermore, the polypeptide has good safety and application potential, providing a new candidate molecule for the clinical treatment and development of novel drugs for psoriasis.

The application discloses application of a Portulaca oleracea extracellular vesicle in resisting acne and regulating acne microecological flora. The Portulaca oleracea extracellular vesicle PDEVs provided by the application can significantly inhibit growth of Propionibacterium acnes, and has obvious bactericidal effect under a lower MIC condition, has less influence on skin beneficial bacteria, is helpful to maintaining or regulating skin microecological balance, and has the advantage of selectively regulating skin flora. Meanwhile, the PDEVs can significantly reduce mRNA expression levels of inflammatory factors IL-6, IL-8, IL-1beta and TNF-alpha of HaCaT cells under stimulation of inactivated Propionibacterium acnes and intracellular reactive oxygen species ROS levels, thereby reducing inflammatory reaction and oxidative stress reaction; the PDEVs have a synergistic effect in regulating skin microecology, inhibiting inflammatory reaction and relieving oxidative stress, have good biocompatibility and safety, and provide more choices for developing natural source active ingredients for preventing or improving acne.

The application discloses 2-hydroxynicotinic acid-3-bromobenzyl ester as well as a preparation method and application thereof, and a structural formula of the 2-hydroxynicotinic acid-3-bromobenzyl ester is shown in the specification. The 2-hydroxynicotinic acid-3-bromobenzyl ester with a novel structure is obtained by using 2-hydroxynicotinic acid as raw material through an esterification reaction. The 2-hydroxynicotinic acid-3-bromobenzyl esters have low cytotoxicity, can remove active oxygen, have good anti-UVB-induced HaCaT cell oxidative damage activity, and can be used in cosmetics or medicines for resisting skin damage, resisting oxidation and / or resisting aging.

This invention discloses an active polypeptide NP-VV-3 that promotes oral ulcer healing and its applications, belonging to the field of biomedical technology. The amino acid sequence of the active polypeptide NP-VV-3 is: VVPAFVLLKKAIRIMFKRNC. In in vitro experiments, polypeptide NP-VV-3 significantly promoted scratch closure of human immortalized keratinocytes at concentrations as low as 1 nM, demonstrating a dose-dependent healing-promoting ability. Transwell migration assays further confirmed that polypeptide NP-VV-3 significantly enhanced the migration ability of HaCaT cells at concentrations of 1 nM and 10 nM, indicating that it can accelerate wound repair by promoting epithelial cell migration. The polypeptide NP-VV-3 provided by this invention provides an important candidate molecule for the development of novel, highly effective, and low-irritation drugs for the treatment of oral ulcers.

The application discloses a water stress protein WSP derived from Nostoc commune and application of the water stress protein WSP in skin protection, and belongs to the technical field of biotechnology and skin care. The amino acid sequence of the water stress protein WSP is shown as SEQ ID NO: 2, and the DNA sequence for coding the WSP protein is shown as SEQ ID NO: 1. The WSP protein of the application can significantly improve the survival rate of skin cells (such as HaCaT cells) under extreme dehydration conditions, protect the activity of key metabolic enzymes (such as lactate dehydrogenase), and effectively reduce the level of intracellular reactive oxygen species, and the comprehensive protection efficiency is superior to that of the existing moisturizing agent hyaluronic acid. The WSP protein provided by the application can be applied to skin care products such as face cream, essence and spray as an active ingredient, and is used for relieving dry skin, repairing skin barrier and antioxidation.