157 results about "Injury repair" patented technology

The invention relates to methods and compositions for the differentiation of stromal cells from adipose tissue into hematopoietic supporting stromal cells and myocytes of both the skeletal and smooth muscle type. The cells produced by the methods are useful in providing a source of fully differentiated and functional cells for research, transplantation and development of tissue engineering products for the treatment of human diseases and traumatic tissue injury repair.

The invention provides cells, compositions and methods based on the use of stromal cells to support the proliferation of undifferentiated embryonic or adult stem cells in vitro. The stem cells produced in the method are useful in providing a source of uncommitted or differentiated and functional cells for research, transplantation and development of tissue engineered products for the treatment of human diseases and traumatic tissue injury repair in any tissue or organ site within the body.

The invention provides a cell preparation for treating osteoarthritis and a preparation method thereof. The cell preparation mainly comprises mesenchymal stem cells, differentiation-induced stem cells, platelet-rich plasma and dispersion media thereof. Compared with the prior art, the cell preparation for treating osteoarthritis has the advantages that the mesenchymal stem cells are mixed with the differentiation-induced stem cells and compounded with the platelet-rich plasma and can be dispersed in the joint cavity, so that the immune regulation function of the mesenchymal stem cells can be exerted, the microenvironment of the whole joint cavity can be improved, and inflammation remission and injury repair are facilitated; tissues can be repaired after the differentiated mesenchymal stem cells are implanted into the body, so that the repair process is shortened.

The invention provides an application of a human umbilical cord mesenchymal stem cell complex cell factor in preparing a biological agent for repairing skin injury. The skin injury includes laser burn, thermal burn, cold injury or common trauma skin physical and chemical injury. According to the application, an umbilical cord stem cell complex growth factor is firstly used for the treatment of human skin laser injury; the human umbilical cord mesenchymal stem cell complex cell factor can be preserved in maximal activity and plays a maximal role of injury repair and treatment under a certain preservation condition.

The described invention provides pharmaceutical compositions for treating an infarct area injury and methods of treating or repairing the infarct area injury in a revascularized subject in the aftermath of an acute myocardial infarction resulting from a natural disease process by administering to the subject parenterally through a catheter a sterile pharmaceutical composition containing a therapeutically effective amount of a nonexpanded sterile isolated chemotactic hematopoietic stem cell product as a first therapeutic agent and optionally a therapeutically effective amount of at least one compatible second therapeutic agent. The infarct area-improving amount of the sterile isolated chemotactic hematopoietic stem cell product comprises an enriched population of isolated autologous CD34+ cells containing a subpopulation of potent cells expressing CXCR-4 and having CXCR-4-mediated chemotactic activity such that the enriched population of isolated autologous CD34+ hematopoietic stem cells provides at least 0.5×106 potent CD34+ cells expressing CXCR-4 and having CXCR-4 mediated chemotactic activity.

The present invention relates to pharmaceutical compositions comprising a chemotactic hematopoietic stem cell product comprising an enriched population of CD34+ cells containing a subpopulation of cells having chemotactic activity, methods of preparing these compositions and use of these compositions to treat or repair vascular injury, including infarcted myocardium.

The invention provides a method for manufacturing an artificial soft tissue body carried with a vascular net flow channel. The method comprises the following steps of: firstly designing a soft tissue scaffold model with a vascular structure, layering the soft tissue scaffold model one by one at equal distance, and manufacturing photomask plates of various layers; then uniformly mixing a cell and a collagen solution, and injecting a photocuring hydrogel and a photoinitiator to obtain a photocuring composite solution; injecting the photocuring composite solution to a work table, covering with the photomask plates, curing the photocuring composite solution by adopting a surface exposure technology, and then carrying out curing accumulation layer by layer to obtain a photocured hydrogel soft tissue scaffold with the vascular structure; planting vascular endothelial cells to the vascular structure of the photocured hydrogel soft tissue scaffold, wherein the vascular endothelial cells are attached to the surface of a vascular channel; carrying out static culture and dynamic culture in vitro to obtain the artificial soft tissue body carried with the vascular net flow channel. The method provided by the invention can solve the problems of survival of cells inside a large tissue engineering soft tissue scaffold, and soft tissue scaffold vessel network manufacturing and vascularization in the large injury repair of a soft tissue.

The invention discloses an osteochondral stent as well as a preparation method and application thereof. The osteochondral stent comprises a cartilage layer, an adhesion layer and a bone layer, wherein two sides of the adhesion layer are respectively connected with the cartilage layer and the bone layer; one or more of the cartilage layer, the adhesion layer and the bone layer are of a porous structure. The osteochondral stent can be used for repairing osteochondral defects, can fully capture cells, has a strong osteochondral injury repairing function, and is simple and convenient to operate.

The invention discloses a preparation technology of biphasic bone and cartilage tissue engineering scaffold material. Firstly chitosan and collagen powder are added into acetate solution and laid in a refrigerator to be preserved; then PLA solution is prepared, and bone dust is put into the PLA solution and is uniformly mixed and stirred; then prepared PLA / bone dust solution is injected into a die to be prefrozen; then collagen / chitosan solution is added into the prefrozen die to be prefrozen, and a scaffold sample is obtained after freeze drying; and finally the bone and cartilage biphasic scaffold conducted to freeze drying is taken out from the die. The preparation technology ensures that a bone layer scaffold has the hardness and the strength approximate to that of natural bone tissue, is not only beneficial to the repair of cartilage tissue, but also convenient for clinically operating and fixing the scaffold; and meanwhile, a cartilage layer scaffold is provided with a multilayer structure containing perpendicular pore canals, is convenient for guiding cells on the scaffold to grow at a state close to that of a natural cartilage laminated structure, and improves the injury repair effect of cartilage.

The invention provides a personalized tissue repairing scaffold for bone, cartilage, skin injury and other injury repairing fields. The personalized tissue repairing scaffold is molded by means of a three-dimensional printing technology, and comprises a substrate and a plurality of sustained-release microspheres dispersed in the substrate, wherein each sustained-release microsphere is of a three-layer structure, namely a core layer, an intermediate layer and an outer layer, and growth factor and / or drugs are loaded on the core layer and outer layer of each sustained-release microsphere. According to the characteristics of treatment positions, degradable high-molecular compound hydrogel or degradable high-molecular / inorganic hybrid hydrogel can be further poured into holes of the substrate, and molding is achieved by means of a low-temperature freeze-drying method. The invention further provides a method for preparing the personalized tissue repairing scaffold. The personalized tissue repairing scaffold has excellent biocompatibility and degradability by adopting the sustained-release microspheres with three-layer structures, is used for pulsed sustained release of growth factor and / or drugs to achieve the effect of long-time stable sustained release, and is beneficial to prolonging of sustained-release cycle of growth factors and / or drugs.

The invention relates to methods and compositions for the differentiation of stromal cells from adipose tissue into hematopoietic supporting stromal cells and myocytes of both the skeletal and smooth muscle type. The cells produced by the methods are useful in providing a source of fully differentiated and functional cells for research, transplantation and development of tissue engineering products for the treatment of human diseases and traumatic tissue injury repair.

The invention relates to a bionic stent simulating a natural tendon tissue fiber hierarchical structure and a preparation method of the bionic stent. The material is a multi-hierarchical structure simulating tendon tissue fibers. A double-nozzle spinning head is adopted, positive and negative electric fields are respectively added, nano fibers are received through a rotating funnel and twisted oncore layer yarns, and stable and continuous primary core-spun yarns are prepared. A plurality of primary core-spun yarns are stranded to serve as core layer yarns, nano fibers are electrostatically spun on the outer layers, the obtained secondary core-spun yarns are stranded to serve as a core layer, nano fibers are electrostatically spun on the outer layer, and tertiary core-spun yarns with a fiber multi-hierarchical structure simulating tendon tissues are prepared and used for a tendon repair stent. The bionic stent is beneficial to tendon repair and normal function recovery, is quite suitable to be used as a tendon injury repair and medical instrument material, and has good application prospect; the preparation method is simple and convenient; and the production efficiency is high.

The present invention relates to pharmaceutical compositions comprising a chemotactic hematopoietic stem cell product comprising an enriched population of CD34+ cells containing a subpopulation of CD34+ / CXCR-4+ cells having CXCR-4-mediated chemotactic activity, methods of preparing these compositions and use of these compositions to treat or repair vascular injury, including infarcted myocardium.

The invention relates to a method for preparing human DNA polymerase delta by using a bombyx mori bioreactor. The method comprises the following specific steps of: respectively constructing four subunits of the human DNA polymerase delta onto a donor plasmid; carrying out transposition on Tn7 transposons in transformed escherichia coli DH10Bac / BmNPV (Bombyx mori Nuclear Polyhedrosis Virus) and BmNPV DNA and screening out clones with correct subunits; and then preparing recombinant viruses of the four subunits assembled by different combinations in BmN cells; infecting host bombyx mori; performing the expression and assembly of a multi-subunit protein complex in bombys mori; and collecting the body liquid of infected larvas or homogenizing entirely, and finally separating and purifying to obtain the target product. By using the method disclosed in the invention, the DNA polymerase multi-subunit protein complex which has high specific activity and is assembled correctly can be prepared efficiently in large scale with low cost. The method can be widely applied to the field of researches on some important diseases, especially on cancers, and the basic research on DNA replication and injury repair.

The invention belongs to the field of medical materials for tendon injury repair and discloses a polycaprolactone / methylacryloyl elastin nanofiber composite membrane and a preparation method and application thereof. The preparation method specifically comprises the following steps of grafting methacrylic anhydride to elastin to obtain the methylacryloyl elastin; then mixing polycaprolactone with the methylacryloyl elastin uniformly to prepare a spinning solution; carrying out electrostatic spinning by using the spinning solution to obtain a composite membrane; and soaking the composite membrane by using a photoinitiator solution, carrying out photo-crosslinking under ultraviolet light and then carrying out washing and drying for many times to obtain the polycaprolactone / methylacryloyl elastin nanofiber composite membrane. The preparation method is easy to operate and relatively low in production cost, and the obtained composite membrane is good in mechanical property and biocompatibility and can be used for tendon tissue engineering and tissue repair.

The invention discloses an ischemic-myocardium-targeted VEGF (vascular endothelial growth factor) recombinant protein. The invention provides a fusion protein which is composed of a VEGF 165, a targeted polypeptide IMT and a connecting peptide for connecting the VEGF 165 and targeted polypeptide IMT. The experiment proves that the brand-new ischemic-myocardium-targeted VEGF protein IMT-VEGF designed in the invention can be targeted to the infarct zone through intravenous injection so as to increase the VEGF effective local concentration. The ischemic-myocardium-targeted VEGF recombinant protein has wide application prospects in targeted therapy, and can be targeted to the ischemic myocardium tissues through intravenous injection to promote the injury repair and cardiac function restoration.

The invention discloses nourishing, moisturizing and injury-repairing lip pomade and a preparation method thereof. The lip pomade contains emu oil, aloe oil, sea buckthorn fruit oil, snake oil, palm oil, coconut oil, borage oil, horse oil, lard oil, madder infused oil, beewax, shea butter, mango fat and essence. The lip pomade is high in safety; active substances in natural drug are adopted, and proportion of oily materials is adjusted properly, so that the lip pomade is not oily, and lips look like more moist and tender after being smeared; natural beewax is adopted, so that the lip pomade is healthier and more environment friendly and has excellent effect on nourishing, moisturizing, repairing injury and clearing heat for detoxification, decrustation caused by long-time makeup is cured effectively, and the lip pomade has obvious effect on repairing lip skin injury such as seasoning crack. The lip pomade has functions of isolating, protecting from sun and moisturizing and can play a role in caring lips in summer.

The invention belongs to the technical field of medical instruments and mainly discloses a plasma therapeutic handle component comprising: a handle and a therapeutic head detachably connected to one end of the handle. The other end of the handle is connected to an external therapeutic host. The therapeutic head includes an electrode tip including an electrode array. The electrode tip generates plasma on the electrode array by being connected to a plasma power source in the external therapeutic host. The plasma therapeutic handle component generates and outputs plasma by the electrode array, thereby ensuring a uniform plasma output. Clinically, the plasma therapeutic handle component can be applied to a plasma therapeutic device with uniform emission for tooth bleaching, tissue injury repair, skin disease treatment, sensitive skin treatment, onychomycosis treatment, and acne treatment.

The invention belongs to the technical field of tree protection and relates to a tree root injury repairing method. The method comprises the following steps in sequence: A, sterilizing and disinfecting the wound of a root system; B, applying a rooting agent or rooting powder solution to a wound part; and C, infusing nutrient solution to root and stem parts. The tree root injury repairing method, provided by the invention, has the benefits that the operation is simple, the implementation is quick, the tree root injury repair period is short, an applied drug is non-toxic and is low in residual quantity, the cost is low, and the injured root system of a tree can be repaired timely to recover the normal life vitality of the tree. The tree root injury repairing method has good application prospect in the technical field of tree protection.

The invention discloses a multi-component composition for tooth sealing treatment. The composition comprises solid-phase powder and blending liquid, wherein the solid-phase powder contains a bioactivecomponent and an X-ray blocking agent component; the bioactive component contains the following chemical substances: 5-90 parts of beta-dicalcium silicate, 5-80 parts of alpha-tricalcium phosphate and 2-10 parts of bioglass; the solid-phase powder is nanoscale to micron-scale superfine powder, mesoporous structures exist in bioglass particles and on the surfaces of the bioglass particles, and thepore diameter of mesopores is 5-48 nanometers; and the blending liquid is an aqueous solution containing calcium ions, phosphate ions and / or silicate ions and the like. According to the multi-component composition for tooth sealing treatment, paste formed by mixing the solid-phase powder part with the blending liquid can be implanted into the root canal and periapical position of a tooth, then the paste can be automatically cured and seal the whole injury area, bacterial intervention can be prevented and treated, bleeding can be stopped, injury repair can be accelerated, and the composition has a great practical value.

The invention discloses a stretch repair process of a hydraulic cylinder piston rod, and relates to the technical field of hydraulic equipment, which solves the problems of bad repair effect and environmental pollution of an existing electroplating repair process of the hydraulic cylinder piston rod. The stretch repair process comprises the following steps of (1) completely turning an original electroplated layer from the surface of a piston rod by using a lathe, and determining the turning depth according to the thickness of the original electroplated layer; (2) cleaning the surface of a damage with trichloroethylene or perchloroethylene; (3) performing sand blasting treatment on the surface of the damage of the piston rod; (4) sending metal alloy powder to the surface of the damage of the piston rod; (5) irradiating the surface of the damage of the piston rod with laser, so that the metal alloy powder is molten so as to form a repair layer; (6) insulating the repaired piston rod, andslowly cooling to room temperature; and (7) further processing the surface dimension and roughness of the piston rod by using turning and polishing equipment. The repair process has the advantages ofhigh strength of the repair layer, long service life and no pollution.

The invention discloses a medicine-loaded nanoparticle vector and temperature-sensitive gel compounding system and a preparation method thereof. The preparation method includes the following steps that nanoparticle vectors are prepared; medicine molecules are connected with amino groups or hydroxyl groups at the ends of polyamidoamine dendrimers in a covalent bonding mode, and a medicine-loaded polyamidoamine dendrimer polymer is prepared; temperature-sensitive gel is prepared, the temperature-sensitive gel is a PLGA-PEG-PLGA copolymer, PLGA is a lactide-glycolide copolymer, and PEG is a polyethylene glycol copolymer; a copolymer solution is prepared by putting the PLGA-PEG-PLGA copolymer into a solvent; and under the condition of stirring, the medicine-loaded polyamidoamine dendrimer polymer is added into the copolymer solution, stirring is continued, and the medicine molecule-loaded nanoparticle vector and temperature-sensitive gel compounding system is obtained. The medicine-loaded nanoparticle vector and temperature-sensitive gel compounding system has a dual slow release function, and can stimulate synovial membrane mesenchymal cells to develop into cartilage cells, relieve the inflammation of a cartilage injured part, and promote the injury repair process of articular cartilages.

The invention discloses a medicinal composition and a preparation method thereof. The medicinal composition consists of the following raw materials in parts by mass: 10 to 20 parts of cuttlebone, 15 to 18 parts of coptis root, 15 to 18 parts of licorice, 15 to 20 parts of immature bitter orange, 15 to 17 parts of aconite, 15 to 17 parts of costustoot, 15 to 17 parts of radix paeoniae alba, 8 to 10 parts of largehead atractylodes rhizome, 8 to 10 parts of bletilla striata, 10 to 15 parts of codonopsis pilosula and 10 to 15 parts of bulbus fritilariae. The medicinal composition has the effect of repairing gastrointestinal mucosa injuries.

The invention relates to a machine-transplanted rice long-acting weed-closing and adverse-resistant green returning fertilizer granule, which comprises a compound fertilizer, an adhesive, a rooting agent, fulvic acid and a weed-closing agent. In the compound fertilizer, nitrogen, phosphorus and potassium are in a weight part ratio of 15:15:15, 15:10:10, or 19:7:14; the adhesive is pregelatinized starch, xanthan gum, sesbania gum, konjac gum, polyacrylamide, hydroxypropyl methyl cellulose, or carboxymethylcellulose; the rooting agent can be indoleacetic acid, indolebutyric acid, indole-3-butyric acid potassium, naphthylacetic acid or sodium naphthalene acetate; and the weed-closing agent can be butachlor, oxadiazon, pendimethalin, oxyfluorfen and the like. According to the invention, high polymer adhesive particles are prepared into a glue solution with clear water, compounding materials are crushed and then mixed evenly, and then the mixture is subjected to granulation molding by a double-roll extruding granulator, and the processing process is simple. The materials are prepared into dry granules that are rich in nutrients and can promote regulation, energy, and planting injury repair and the like, the granule are sprayed into rice field and become loose after water absorption. The granules provided by the invention can completely replace the fertilizers and herbicides produced by the traditional method.

The invention discloses a medicinal fatheadtree branchlet or bark alkaloid new compound with the function of promoting endothelial injury repair. The compound is prepared by a method including the steps: crushing medicinal fatheadtree branchlet or bark stems, adding water, performing soaking and extracting, collecting extraction liquid and performing vacuum concentration to obtain medicinal fatheadtree branchlet or bark extracts; extracting the extracts to obtain a dichloromethane portion and an ethyl acetate portion; feeding the ethyl acetate portion to a silica gel column; performing elution by taking dichloromethane-methanol with the volume ratio of 95:(5-10):90 as an elution solvent; combining similar portions by TLC (thin-layer chromatography) analysis; feeding a preparative liquid phase, taking methanol water as an eluent and performing liquid chromatography preparation to obtain the medicinal fatheadtree branchlet or bark new compound. Based on a cell repair related mechanism, under theoretical guidance, the proliferative activity of the medicinal fatheadtree branchlet or bark new compound is intensively studied, and experimental study indicates that the medicinal fatheadtree branchlet or bark new compound can obviously promote proliferation of HUVEC (human umbilical vein endothelial cell) cells and endothelial cell scratch repair and can be used for preparing a drug for controlling inflammatory endothelial injury.

The invention provides a wound protection composition, which is capable of preventing wound infection and promoting injury repair. The composition is free from antibiotics, and the composition consists of a dressing tenacity enhancing component, cell injury repair factors, a bactericidal-bacteriostatic molecule pair and a solvent. The composition can form a transparent liquid layer on the outer surface of a wound, and the transparent liquid layer can be rapidly transformed into a dressing which is thinner than 0.01mm. The composition is capable of forming a film easily and the film material is high in tenacity, and the dressing, which is formed on the outer surface of the wound, is high in air permeability and waterproof performance and is capable of effectively preventing such foreign matters as bacteria, dust and the like from invading into the wound so as to prevent infections; meanwhile, the dressing has such effects as killing bacteria, stopping bleeding, relieving pain and the like; and the composition is free from sensitization and hormone, and the composition is capable of effectively promoting the repair of damaged cells and wounds.

The invention provides a method for detecting cell injury repair ability and a special chip thereof. The chip consists of 2 to 99 identical cell culture structure units, wherein the structure units are connected with each other through a public cell sample pool, and each structure unit comprises a column-shaped structure, a baffle structure and a waste liquid pool. The invention creatively integrates cell culture, defect region formation, injury-repair process starting and other functional units on one chip, and adopts the function of the column-shaped structures in blocking cells to form cell defect regions with the same shape and size. Compared with the prior scratch method, the method has the advantages of simplifying artificial operation, providing exactly the same initial defect region, reducing cell consumption, having high flux and ensuring that the injury repair process is easy to photograph in fixed positions.

The invention relates to a tear drainage reconstruction system that can be regularly degraded. The reconstruction system is made by adopting polydioxanone (PDO) that is a degradable polymeric biomaterial. The system comprises an upper lacrimal duct part, a lower lacrimal dust part, a main lacrimal duct, a concave dacryocyst part and a nasolacrimal duct part. The system has good flexibility, certain elasticity, extensibility, breaking strength and compression strength performance and is a lamella micro-porous tubular structure that integrates stable nasolacrimal duct supporting function, 'tears pump' function, dacryocyst drainage function, 'drug control-release' function, the function of accelerating the fade-away of chronic inflammation of a lacrimal duct and the repairing function of mucous membrane injury of the lacrimal duct together. The reconstruction system has the advantages that an implanting method is a simple and convenient, the fixation in the lacrimal duct is the stable and reliable and the iatrogenic lacrimal duct injury can not appear, the regular degradation can be realized without secondary take-out and the reconstruction system is suitable for various lacrimal duct obstruction diseases and has high curative ratio of the lacrimal duct obstruction, especially, the reconstruction system has the stable and reliable medium-to-long term effect. The tears drainage reconstruction system currently is the only perfect material for high-degree lacrimal duct reconstruction that combines the physiological drainage of the tears with the pathological mechanism of lacrimal injury repair.

The invention discloses a novel nanometer drug carrier loaded with methyllprednisolone. The novel nanometer drug carrier comprises methyllprednisolone and a carrier, wherein the carrier is an ibuprofen modified dextran nanometer microsphere, the particle diameter of the novel nanometer drug carrier loaded with methyllprednisolone is 120 to 160 nanometers, the preparation method of the novel nanometer drug carrier loaded with methyllprednisolone comprises two steps of synthesizing the ibuprofen modified dextran nanometer microsphere and preparing novel nanoparticles loaded with methyllprednisolone. The novel nanometer drug carrier loaded with methyllprednisolone disclosed by the invention has the advantages that the nanometer microsphere synthesized by ibuprofen modified dextran is taken as the carrier, so that methyllprednisolone can be effectively and targetedly delivered to injured parts, the half-life of methylprednisolone drugs can be effectively prolonged, and methyllprednisolone and ibuprofen can play a synergistic role on promotion of central nervous system injury repair.

The invention provides sunscreen and after-sun repairing skin care composition and a cosmetic containing the same. The composition comprises a rhEGF (recombinant human epidermal growth factor), hydrolyzed soy protein, purslane herb extract, Chinese magnoliavine fruit extract and rhizoma kaempferiae extract. The weight content of the composition in the cosmetic is 1%-2%. The weight content of the rhEGF in the sunscreen cosmetic is only 1-10 mu g / ml and is far lower than the conventional addition amount, the rhEGF is compounded with the hydrolyzed soy protein, the purslane herb extract, the Chinese magnoliavine fruit extract and the rhizoma kaempferiae extract to realize synergistic effect, so that injured cells can be rapidly repaired, skin injury caused by ultraviolet radiation is relieved, further, abnormal increase of melanophores of a skin basal layer can be reduced, melanin synthesis is blocked, black spots growing on the after-sun skin are reduced, gene mutation factors of the injured cells are eliminated, light aging is prevented, and accordingly, the composition has an ultraviolet ray preventing function and an after-sun injury repairing function.