4 results about "Nuclear Polyhedrosis Virus" patented technology

The application discloses a production method of a high-efficiency strain of Bupalus piniarius nuclear polyhedrosis virus, and belongs to the technical field of biological pesticide production. 8 The virus yield of the high-efficiency strain of Bupalus piniarius nuclear polyhedrosis virus is 6*10 The virus loss rate is reduced by 1.4% by adding 0.1% detergent before centrifugal purification of the virus, and the high yield of the virus is further ensured.The insecticidal effect of the high-efficiency strain produced by the method is increased by more than 64.1% than that of an ordinary strain, and the high-efficiency property is continuously maintained.The method has a very beneficial effect on the production of the high-efficiency strain of Bupalus piniarius nuclear polyhedrosis virus, and provides a solid guarantee for popularization and application of the high-efficiency strain.

The application discloses a cotton bollworm nuclear polyhedrosis virus spike period targeted synergistic adjuvant, a pesticide preparation and application, relates to the technical field of agricultural biological pesticides, and the composite adjuvant is prepared from ultraviolet protection and shielding materials, film-forming materials, midgut infection promoters, penetration and spreading agents, system compatible components and carriers.The adjuvant is applied by unmanned aerial vehicle top spraying, and after being compounded with HaNPV, the deposition rate of the virus on the corn tassel can be increased by 3.2 times, the retention amount is increased by 5.62 times, the destruction rate of the midgut peritrophic membrane of the cotton bollworm larvae is more than 90%, and the mortality of the 3rd instar cotton bollworm larvae is increased to 87.72%.The application solves the two technical bottlenecks of the virus pesticide, i.e., 'difficult coverage of the tassel target' and 'low midgut infection efficiency', and provides key technical support for green and precise prevention and control of the cotton bollworm in the corn tassel period.

The present invention relates to synthetic designs or chimeric proteins for pseudotyping baculovirus (Autographa californica nuclear polyhedrosis virus) with the rabies virus glycoprotein G (gG) on its surface (Bac::gG-FL), which can be used in rabies vaccine formulations. The chimeric protein is designed from a gene cassette containing genetic fragments from the ectodomain of the Pasteur variant rabies virus glycoprotein G, a 7-amino-acid connector sequence (GGGGSGG), as well as transmembrane (TM) and cytoplasmic (CT) regions of the baculoviral protein gp64, the arrangement of which in the designed gene cassette is shown in Figure 1.

The application belongs to the field of biological pesticides, and provides application of HearNPV-miR-56 in cotton bollworm control, wherein the nucleotide sequence of the HearNPV-miR-56 is CGGUAACAGAAGCGGUCACG. The HearNPV-miR-56 can improve virus replication amount of cotton bollworm nuclear polyhedrosis virus infected larvae, thereby promoting death, and can be used for developing a cotton bollworm nuclear polyhedrosis virus synergist, and has potential application prospect in biological control of cotton bollworm.