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144 results about "Nuclear Polyhedrosis Virus" patented technology
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The nuclear polyhedrosis virus (NPV), part of the family of baculoviruses, is a virus affecting insects, predominantly moths and butterflies. It has been used as a pesticide.
Broad spectrum bacilliform virus insecticide made of substitution host
The invention provides a broad-spectrum rod-shaped viral pesticide produced by substitute hosts, the components and the weight proportion of which are: 20 billion PIB / ml virus suspension 2.5 to 15 percent, 60nm silicon dioxide 0.1 to 0.5 percent, aromatic amino acid 0.1 to 0.15 percent, refined fluorescent whitening agent 0.5 to 1 percent, sodium lignin sulfonate 0.1 to 1 percent, chlorfluazuron 0.01 to 0.1 percent, glycerol 1.0 to 2.0 percent, and remaining no sterilized water; the virus suspension is received by the infection of nuclear polyhedrosis virus of cabbage armymoth and the substation for the host---cotton bollworm or beet armyworm. The pesticide not only maintains the biological pesticide properties of viral pesticides and has no harms to human beings and higher animals, but also is suitable for the the massive employments on vegetables, and has the wide insect disinfestation spectrum. Therefore, a broad-spectrum viral pesticide can prevent and control various harmful insects while with only a rod-shaped virus. The invention has the advantages of good insecticidal effect, fast insecticidal speed, and very good application prospect.
Owner:JIANGXI NEW DRAGON BIOTECH
Method for producing porcine circovirus type II recombinant capsid protein subunit vaccine by utilizing silkworm bioreactor and products thereof
ActiveCN101920012AGreat advantageProtein, high post-translational modification efficiencyViral antigen ingredientsVirus peptidesProtein targetTransfer vector
The invention provides a method for producing porcine circovirus type II capsid protein by utilizing a silkworm bioreactor, and belongs to the field of biotechnology. In the method, by taking a bombyx nuclear polyhedrosis virus as a vector, porcine circovirus type II capsid protein genes are integrated into a polyhedrosis promoter of the bombyx nuclear polyhedrosis virus so as to express target protein by a mode of the homologous recombination of a homologous arm of the nuclear polyhedrosis virus in a transfer vector and bombyx nuclear polyhedrosis virus (BmNPV) genes; a recombinant bombyx nuclear polyhedrosis virus containing target protein genes is obtained by a plaque sieving method, and the target protein is expressed in large scale by using a bombyx bioreactor so as to prepare a subunit vaccine containing recombinant porcine circovirus type II capsid protein; and piglet infection experiments verify that the subunit vaccine has the excellent immune protective effect. The method has the characteristics of high expression efficiency, good activity of the target protein, low production cost and the like, and is suitable for large-scale production.
Owner:PU LIKE BIO ENG +1
Insecticide suspension of beet noctuid polyhedron virus
InactiveCN1387764AMaintain biopesticide propertiesFast insecticideBiocideAnimal repellantsPesticide residueTyrosine
The present invention discloses beet noctuid nuclear polyhedrosis virus insect killing suspending agent, in which the virus bearer content is 5-20 hundred million PIB / ml., itis composed of chlorbenzuron analogue chlorfluazuron, aromatic amino acid tyrosine, fluorescent whitener, dispersant, glycerin, sterilized water. The present invention is harmless to man and livestock and other higher animals, has no pollution to environment and has no pesticide residue.
Owner:WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
Porcin circovirus type 2 (PCV2) recombinant baculovirus construction method and subunit vaccine preparation method thereof
InactiveCN102352347AViral antigen ingredientsMicroorganism based processesOpen reading frameViral vector
The invention relates to a porcin circovirus type 2 (PCV2) recombinant autographa californica multicapsid nucleopolyhedrovirus construction method and a subunit vaccine preparation method thereof. The novel recombinant lucerne fork vein noctuid nuclear polyhedrosis virus construction method comprises the steps that: the main surface antigen which expresses the PCV2 is used to prepare the subunit vaccine with better immunity. Specifically, in the carrier expression system of the PCV2 recombinant autographa californica multicapsid nucleopolyhedrovirus, a hepatitis E virus open reading frame (ORF2) gene of the PCV2 is directionally inserted, so the gene efficiently expresses in insect cells and has good immunogenicity, the prepared corresponding subunit vaccine can stimulate pigs to generate the protective immunoreaction against the attack of the strong virus of the PCV2.
Owner:ZHEJIANG YEBIO BIOTECH
Method for select-breeding anti-virosis Bombyx mori varieties by utilizing Thailand Bombyx mori disease-resistant gene
InactiveCN101558754AIncrease resistanceEasy to operateAnimal husbandryNuclear Polyhedrosis VirusBombyx mori
The invention discloses a method for select-breeding anti-virosis Bombyx mori varieties by using Thailand Bombyx mori disease-resistant gene. The method comprises the steps of: selecting the present Bombyx mori varieties of 871 and 872 used both in spring and autumn in China as female parent and using the introduced Thailand tropical disease-resistant variety NL as male parent; respectively hybridizing and leading-in the disease-resistance gene; hybridizing second generation and third generation silkworm and respectively adopting mixed batches rearing for breeding; hybridizing each generation beginning from a second age and screening by adopting 'population feeding' NPV (nuclear polyhedrosis virus) and a method with the concentration of virus feeding 'increasing age by age in a gradient way'; taking the Bombyx mori varieties 871 and 872 as recurrent parent, respectively adopting reserve seed antivirus silkworm individual to backcross, using a method of the combination of 'backcrossing introduction' and 'generation directed virus feeding screening breeding' to obtain an NL pure and mild system with comparatively stable genetic character by a plurality of backcrosses and virus feeding screening. In the process of select-breeding, various economic characters and production properties of the system are consolidated and improved and the objects of pure breeds of 871NL and 872NL are bred. The obtained antivirus Bombyx mori variety has good economic characters, is sturdy and easy to keep.
Owner:ANHUI AGRICULTURAL UNIVERSITY
Diagnosis of penaeus monodon-type baculovirus by PCR
InactiveUS6284455B1Sugar derivativesViral antigen ingredientsConserved sequenceNuclear Polyhedrosis Virus
This invention relates to the methods of detecting Penaeus monodon baculovirus (MBV). Two methods are established: the first one is a polymerase chain reaction (PCR) and the second one is an ELISA. For the PCR method, two sets of primers are designed. The first set of primers is designed from the conserved sequences of nuclear polyhedrosis viruses (NPVs) DNA polymerase genes. The second set of primers is designed from the genomic DNA of MBV. The antibody for ELISA is an antiserum against the occlusion bodies of MBV.
Owner:YA LI HSU
Preparation method of a recombinant nuclear polyhedrosis virus capable of infecting tea geometrids
InactiveCN102260690AMeet needsProduction is easy to controlBiocideViruses/bacteriophagesCompetent cellNuclear Polyhedrosis Virus
The invention discloses a preparation method of recombinant nuclear polyhedrosis virus (NPV) which infects ectropis oblique. The preparation method comprises steps that: (1) recombinant vectors pFastBacDual-polh-helicase are prepared; (2) the recombinant vectors pFastBacDual-polh-helicase are transformed into E.coli DH10 BmBacmid competent cells; the cells are cultivated on a cultivating plate; positive plaques are obtained through blue-white selection; and recombinant BmBacmid-polh-helicase DNA is extracted from the positive plaques; (3) the ecombinant BmBacmid-polh-helicase DNA is transfected to domestic silkworm cultivated cells; cultivated cell supernatant is collected; and recombinant virus BmEoNPV is obtained; (4) the recombinant virus BmEoNPV is used for injection; and recombinant virus polyhedron is obtained. The recombinant nuclear polyhedrosis virus is sprayed on tea tree leaves, such that the ectropis oblique can be prevented from harming the tea trees. The preparation method also has advantages of low cost and controllable productions.
Owner:溧阳市民生农业科技园有限公司 +1
Human interleukin-12 recombinant insect virus strain and its prepn
InactiveCN1357622AEfficient formationViruses/bacteriophagesFermentationWhite blood cellInfective disorder
The present invention discloses a human interleukin-12 recombinant insect virus strain, the recombinant Autographa california multiple nuclear polyhedrosis virus AcNPV-hIL12 virus strain, CCTCC No.2 V200108 and its preparation. The recombinant virus strain is inserted into human interleukin-12 expressing box, and the expressing box includes double promoter sequence, P35 subunit DNA encoding sequence of human interleukin-12 and P40 subunit DNA encoding sequence. P35 subunit gene locates in the downstream of Polyhederin and P40 subunit gene locates in the downstream of P10 promoter. The present invention can express human interleukin-12, and the cell factor is safe and reliable to human body and suitable for treating tumor bacteria, virus and various infections diseases.
Owner:WUHAN UNIV
Composite biological insecticide
The invention discloses a composite biological insecticide and a preparation method thereof, which belongs to the technical field of biological pesticide. The state prodenia litura nuclear polyhedrosis virus Suzhou No.1 (SpltNPV-sz-1) is stored in China Typical Culture Conservation Center on June 25, 2008 with the collection number of CCTCC No: V200806. The composite biological insecticide comprises the following components in weight: 1 to 10 percent of prodenia litura NPV-sz-1 raw powder, 0.1 to 3 percent of methylamino abamectin benzoate raw powder, and the balance being functional addition agents and solvent. The composite biological insecticide and the preparation method thereof have the characteristics of high efficiency, broad spectrum and no pollution; the composite biological insecticide is suitable for preventing and controlling lepidopterous pests and mites such as the prodenia litura, diamondback moth and diaphania indica, widens the desinsection range, improves the desinsectio effect, delays the generation of insecticides resistance of insects, plays the continuous natural control role, reduces the usage amount of chemical pesticide, lowers the cost, protects the entironment and has wide application prospect.
Owner:SUZHOU UNIV +1
Method for finding protein interaction with baculovirus PCNA (Proliferating Cell Nuclear Antigen)
InactiveCN103713037AEasy to findQuick searchVirus peptidesMaterial analysis by electric/magnetic meansProliferating cell nuclear antigenMass spectrography
The invention discloses a method for finding a protein interaction with baculovirus PCNA (Proliferating Cell Nuclear Antigen). The method is characterized by comprising the following steps of cloning full-length DNA of an autographa californica nuclear polyhedrosis virus (AcMNPV) PCNA gene by utilizing the means such as molecular cloning, and DNA sequencing, purifying the full-length DNA, coupling the full-length DNA to an affinity column, and fining the interaction protein by adopting the mass spectrography. The method can be used for finding and functionally studying interaction protein of the baculovirus PCNA.
Owner:JIANGSU UNIV
IBDV (Infectious Bursal Disease Virus) VP2 protein and IBD subunit vaccine
InactiveCN103172709AEasy constructionImprove immunityViral antigen ingredientsVirus peptidesNuclear Polyhedrosis VirusSubcutaneous injection
The invention relates to an IBDV (Infectious Bursal Disease Virus) VP2 protein. The mass production of the expressive IBDV VP2 protein in a silkworm larva or a pupae is succeeded for the first time by performing the subcutaneous injection of a restructured autographa californica nuclear polyhedrosis virus into the silkworm larva or the pupae. However, in the prior art, the expression of the restructured autographa californica nuclear polyhedrosis virus containing the IBDV VP2 in the silkworm larva or the pupae always cannot be performed successfully. The invention further provides a method for producing the IBDV VP2 protein and an IBD subunit vaccine by utilizing a silkworm bioreactor. The IBDV VP2 protein can be applied to the preparation of the vaccine for treating and preventing the IBD as well as the preparation of an IBD diagnostic reagent.
Owner:PU LIKE BIO ENG
Hyphantria cunea viral insecticide and preparation method thereof
InactiveCN104430545AImprove the protective effectLong duration of actionBiocideAnimal repellantsNuclear Polyhedrosis VirusFluorescence
The invention provides a hyphantria cunea viral insecticide and a preparation method thereof. The preparation method comprises the following steps: feeding hyphantria cunea by virtue of artificial feed, preparing a nuclear polyhedrosis virus precipitate, and adding trace inorganic nano-materials, aromatic amino acids and a small amount of refined fluorescent whitening agent into the virus precipitate. According to the preparation method, the viral insecticide is complete in components and high in suspension property, the quality guarantee period is prolonged, the insecticide has a good protective effect against ultraviolet light during field application, the virus duration is long, and the control effect is greatly improved.
Owner:INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI
Use of cnidiadin as insect virus synergist in biological control
InactiveCN1488252AReduce dosageHighly synergistic activityBiocideAnimal repellantsEcological environmentNuclear Polyhedrosis Virus
The invention is an application of cnidiadin as insect virus synergist to the biologic prevention and cure. Cnidiadin derives from common cnidium fruit extract, when used singly, has not pesticidal activity, but when associated with insect nuclear polyhedrosis virus (AcNPV, SINPV, MbNPV, etc.) and granulosis virus (XcGV, etc.), can remarkably enhance the infection capability of insect virus to pest. The dosage is low (1-228mg / L), and the concentration lower, the synergistic action stronger, safe to entironment.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
Method for preventing and treating vegetable noctuid using attractant for spreading virus
InactiveCN101502252APromote natural popularityImprove insecticidal effectBiocideAnimal repellantsNuclear Polyhedrosis VirusKaryotype
The present invention discloses a method for preventing vegetable noctuid through propagating virus by attractant. According to the method, a noctuid attractant is used for inducing the imago to carry and propagate the nuclear polyhedrosis virus so that the virus propagates vertically and horizontally in the host group. The progeny larvae falls ill and dies after the female which copulates with the virus-taking male or the female carrying the virus spawns. The method of the invention can promote the natural propagation of virus in the field, reduces the dosage of virus insecticide and increases the effect of insect disinfestation with virus. Compared with the currently submerged virus releasing, the method of the invention has evident advantage and has great market development potential.
Owner:SHANGHAI ACAD OF AGRI SCI
Ectropis oblique warren nuclear polyhedrosis virus insecticidal suspending agent and production method thereof
InactiveCN104430539AAvoid drug resistanceAvoid reduction in the number of natural enemiesBiocideAnimal repellantsFluorescencePolyvinyl alcohol
The invention mainly aims to provide an ectropis oblique warren nuclear polyhedrosis virus insecticidal suspending agent and a production method thereof. The insecticide consists of virus suspension, silicon dioxide, aromatic amino acids, a refined fluorescent whitening agent, sodium lignin sulfonate, polyvinyl alcohol, chlorfluazuron, glycerin and sterile water. The production method comprises the following steps: paired spawning on pupas by virtue of emerged adults, sterilizing the eggs, hatching larvae, feeding the larvae by using artificial feed, inoculating ectropis oblique warren virus on the feed surface to feed, grinding and filtering the larvae infected with the virus, centrifuging filtrate, collecting precipitates, and preparing the corresponding insecticide. By utilizing the insecticide, plant diseases and insect pests of ectropis oblique warren can be effectively controlled, the control cost is low, the environment friendliness is promoted, and the problems that the drug resistance of injurious insects is enhanced, the natural enemy number is reduced and pesticide residues in tea leaves are increased due to use of lots of chemical pesticides are solved.
Owner:INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI
Rabbit hemorrhagic disease virus recombinant subunit vaccine and preparation method thereof
ActiveCN103789274AHigh expressionAvoid it happening againViral antigen ingredientsMicroorganism based processesDispersityProtein detection
The invention relates to a rabbit hemorrhagic disease virus recombinant subunit vaccine and a preparation method thereof. A vaccine production strain is a recombinant autographa californica nuclear polyhedrosis virus CGMCC No.8052 (China General Microbiological Culture Collection Center Number 8052), an expression vector adopted by the strain is a secretory expression vector and is provided with six histidine tags easy for protein detection and analysis; the strain is used as the production strain for producing the rabbit hemorrhagic disease virus recombinant subunit vaccine; the vaccine is free from viral genome, animal safety hazard and potential dispersity, is high in immunity pertinence, and can be used for exciting immunized animals to generate sufficient immune antibodies and long protective efficacy. RHDV-like (rabbit hemorrhagic disease virus-like) particles are produced according to the technology provided by the invention, the hemagglutination titer of virus liquid obtained from a 1000L bioreactor is (1 to 32768) to (1 to 262144), the protein expression quantity of the RHDV particles is about 150mg / L, 1L of semi-finished virus liquid can be prepared into 5,000-100,000 standard vaccines, and the preparation efficiency is higher than the domestic technical level by 8-20 times; the recombinant subunit vaccine is added with an adjuvant, so that the immune effect of the vaccine is improved; and the vaccine is a novel vaccine remarkably superior to an inactivated tissue vaccine produced in the prior art.
Owner:QILU ANIMAL HEALTH PROD
Fast donor plasmid carrying cotton bollworm single particle embedded nuclear polyhedrosis virus gene and P10 gene promotor sequence and construction method
A fast doner plasmid with singly embedded polyhedronvirus gene of bollworm and P10 gene promoter sequence is configured through inserting exogenous gene in polycloning site under control of P10, transfecting it to bollworm cell by inserting the exogenous gene and polyhydrous gene in the shuttle expression carrier, collecting recombinant virus particles, transfecting again, and detecting the exogenous gene expression level. Its advantages is short period (7-14 days).
Owner:WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
Cotton bollworm single particle embedded nuclear polyhedrosis virus shuttle expression carrier and preparation method
A single embedded polyhedronvirus shuttle expression carrier of bollworm is configured through inserting the low-copy bacterium F replicon, kanamycine resistant gene and beta galactosidase gene to the polyhedrom gene site of the virus genom. The doner plasmid is used to transfer the exogenous gene to the insetion site of transposon in the said shuttle expression carrier. After bollworm cell is transfected by said DNA, the infections recombinant virus particles are formed to express exogenous gene under control of polyhedron promoter and P10 promoter.
Owner:WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
Synergistic method of insect growth regulator and nucleopolyhedron virus
InactiveCN1887093AImprove damage control effectImprove the level of biological controlBiocideChemosterilantsNuclear Polyhedrosis VirusNeurotoxicity
The present invention discloses the synergistic method for nucleopolyhedron virus. For sake of overcoming the demerits of single insect virus, such as slow pesticidal speed, low toxicity, etc, insect growth regulator chlorfluazuron is made to cooperate with insect virus so as to raise the pest controlling effect obviously. Compared with the traditional method of raising the pest controlling effect of virus with neurotoxic pesticide, the present invention has the advantages of high synergistic effect, high safety and raised biological control level of pests.
Owner:JIANGSU ACAD OF AGRI SCI
Virus insecticidal suspension agent containing insect hormone insecticide
InactiveCN104542710AGuaranteed stabilityEasy to storeBiocideAnimal repellantsInclusion bodiesNuclear Polyhedrosis Virus
The invention discloses a virus insecticidal suspension agent containing an insect hormone insecticide. The virus insecticidal suspension agent comprises the following components in percentage by weight: 0.1-30% of the insect hormone insecticide, 1-10% of a dispersant, 1-7% of an antifreezing agent, 0.2-10% of a stabilizer, 0.1-5% of a synergist, 0.5-2% of an ultraviolet protecting agent, 0.1-2% of a defoaming agent, 0.1-3% of a pH regulator and the balance of water, wherein the prepared suspension agent also contains an insect virus which is one of or a combination of a nuclear polyhedrosis virus (NPV) and granulosis virus, and the content of an insect virus inclusion body in the suspension agent is 0.5-20 billion PIB / ml. The insect hormone insecticide and a virus insecticide of the virus insecticidal suspension agent disclosed by the invention are subjected to complex pairing, so that the problem of insect resistance can be solved, and the virus insecticidal suspension agent is safe and environmentally friendly.
Owner:JIANGXI NEW DRAGON BIOTECH
Microbial pesticide for preventing and controlling prodenia litura
InactiveCN102726453AReduce pesticide residuesQuality improvementBiocideAnimal repellantsMicroorganismNicotiana tabacum
The invention discloses a microbial pesticide for preventing and controlling prodenia litura. In the microbial pesticide, the prodenia litura larva, prodenia litura nuclear polyhedrosis virus production strain stock solution and fresh tobacco are used as major raw materials, and virus reproduction is performed. The microbial pesticide is prepared mainly by the following steps of: breeding the larva, inoculating the viruses, collecting the larva died of illness and performing crude extraction and filtration to obtain the virus stock solution; mixing the virus stock solution, glycerin and water at a weight ratio of 1:(1.2-4):(2.3-6) to prepare a virus stabilizer; preparing a virus mixture from the virus stock solution and 5% emamectin benzoate at a weight ratio of 1:(1.1-1.25); mixing and dissolving the virus stabilizer and the virus mixture; and adjusting the pH value to 6.8 with dilute sulphuric acid solution to obtain the microbial pesticide. The microbial pesticide disclosed by the invention is used for preventing and controlling the prodenia litura pests on crops such as vegetables, tobacco and the like, has high efficiency and environmental protection effect, and improves the quality and yield of the tobacco.
Owner:HUNAN TOBACCO CHENZHOU +2
Technique for constructing high-virulent nuclear polyhedrosis virus of lepidoptera pest
InactiveCN1804030AInhibition of CS gene activityDiffusion fastViruses/bacteriophagesVector-based foreign material introductionBiotechnologyVirulent characteristics
The technique to modify NPV and construct high-virulence NPV of Lepidoptera pest with modern dsRNA interference molecular biological technique comprises: (1) cloning CS conservative sequence of geometrid; (2) constructing the dsRNA intermediate carrier for the lepidopterid CS gene; (3) constructing the NPV transfer carrier contained CS dsRNA interference sequence; (4) recombining NPV, validating the NPV with PCR or other methods; finally, contrast with wild NPV, evaluating the insect disinfectation capacity of recombined NPV to screen the virus strain with higher virulence and faster diffusion and secondary infection speed and better prevention effect.
Owner:ZHEJIANG UNIV
Manufacturing method and application of insect in-vitro protein interaction detecting system
InactiveCN104991072AEfficient transfectionSimple methodBiological testingFluorescence/phosphorescenceProtein insertionPolyhedral virus
The invention discloses a manufacturing method and application of an insect in-vitro protein interaction detecting system. At the positions of amino acid residues at the 159th bit and the 160th bit, mCherry is divided into a segment NmC and a segment CmC through a PCR method, and then the segment NmC and the segment CmC are each fused with a connecting zone sequence, a c-Myc label and a poly(A) sequence to form four fused segments; the four fused segments are each connected with a pIE-MCS plasmid with an autographa californica multiple nuclear polyhedrosis virus ie1 gene promoter and a gp64 gene poly(A) sequence to establish expression plasmids; the expression plasmids are combined to form an insect cell dimolecular fluorescent complementary detecting system. The method is simple and easy to implement, and the established dimolecular fluorescent complementary detecting system expands the application of an insect cell expression system and provides a convenient and effective technological means for insect proteomics and research on protein interaction between insects and pathogenic microorganisms of the insects.
Owner:NORTHWEST A & F UNIV
Method for using silkworm cultured cell to express antibacterial peptide Cecropin B
InactiveCN101845439ASolve operational problemsFix stability issuesViruses/bacteriophagesGenetic engineeringDideoxynucleotide TriphosphatesTotal rna
The invention discloses a method for using silkworm cultured cell to express antibacterial peptide Cecropin B. The method comprises the following steps: (1) using the total RNA extracted from the fat body cells of wild silkworm chrysalis as template, adopting RT-PCR amplification to obtain wild silkworm antibacterial peptide Cecropin B gene; using 1% agarose gel electrophoresis to perform PCR product analysis to the antibacterial peptide Cecropin B gene, using a PCR purification kit of Qiagene for purification, then cloning the purified PCR product to TA vector pCR2.1 to obtain pCR2.1-Cecropin B, utilizing the dideoxynucleotide chain termination to confirm the correctness of cloned gene order; using restriction endonuclease BamHI and HindIII to digest pCR2.1-Cecropin B and obtain Cecropin B genetic fragments, then cloning rhabdovirus transfer vector pBlueBacHisa in the genetic fragments to obtain reconstituted transfer vector; performing cotransfection of the reconstituted transfer vector and silkworm wild-type nuclear polyhedrosis virus BmNPV DNA in silkworm cultured cell, performing homologous recombination to generate recombinant virus; and (3) inoculating the recombinant virus containing wild silkworm antibacterial peptide Cecropin B gene in the silkworm cultured cell, infecting at 27 DEG C for three days to express the infection, and centrifuging to collect silkworm cultured cell.
Owner:ZHEJIANG UNIV
Qualitative detection method of tea geometrid nuclear polyhedrosis virus
ActiveCN101871944ASolve accurate qualitative problemsStandard production applicationImmunoglobulins against virusesMaterial analysisVirulent characteristicsProtein.monoclonal
The invention relates to a qualitative detection method of a tea geometrid nuclear polyhedrosis virus, which belongs to the technical field of the detection of biological preparations and is characterized by comprising the following steps of: firstly, preparing a polyhedrin monoclonal antibody of the tea geometrid nuclear polyhedrosis virus, then coating a sample to be detected and detecting by using an indirect Elisa method. The invention breaks through a traditional biological assay technology by means of an immunological means and solves the problem of accurate qualification of the tea geometrid nuclear polyhedrosis virus. Compared with a traditional biological assay method, the virulence index detection of the method is finer, the virulence evaluation period is greatly shortened, the method can be widely used for estimating the quality of a virus preparation and specification of the production and the application of a tea geometrid virus.
Owner:TEA RES INST CHINESE ACAD OF AGRI SCI
Domestication method and application of nuclear polyhedrosis virus adapted to spodoptera frugiperda
PendingCN111117972AGood killing effectDrug resistanceBiocideMicroorganism based processesBiotechnologyNuclear Polyhedrosis Virus
The invention discloses a domestication method and application of a nuclear polyhedrosis virus adapted to spodoptera frugiperda. The domestication method includes the following steps: performing UV irradiation on an autographa californica nuclear polyhedrosis virus, applying the treated virus to artificial feed of the spodoptera frugiperda to obtain virus-containing feed, feeding hungry spodopterafrugiperda larvae with the virus-containing feed, collecting poisonous insects or dead insects, performing crushing, performing filtration, and performing centrifugal collection to obtain a free-state autographa californica nuclear polyhedrosis virus; and performing iteration culture and amplification alternately on the free-state autographa californica nuclear polyhedrosis virus by using isolated cells and insects of the spodoptera frugiperda to obtain a domesticated autographa californica nuclear polyhedrosis virus, and improving viral titer of the domesticated virus to the spodoptera frugiperda by an insect cultivating method to obtain the nuclear polyhedrosis virus adapted to the spodoptera frugiperda. The autographa californica nuclear polyhedrosis virus provided by the invention hasthe characteristics of strong pertinence, long control acting time, no side effects, and difficulty in producing drug resistance by pests, and the virus domestication efficiency is fast and efficient.
Owner:SUZHOU MIDI BIOTECH CO LTD +1
Mink enteritis virus recombinant subunit vaccine and preparation method thereof
ActiveCN103820398AImmunity is highly targetedImprove immunityAntiviralsDepsipeptidesProtein detectionPolyhedral virus
The invention relates to a mink enteritis virus recombinant subunit vaccine and a preparation method thereof. The strain that produces the vaccine is recombinant autographa California nuclear polyhedrosis virus (CGMCC No. 8051), the expression vector of the strain is expressed through secretion, comprises six histidine labels, and is prone to be used in protein detection and analysis; mink enteritis virus recombinant subunit vaccine produced by the strain does not contain any viral genome and thus does not have any animal safety hazard or potential infectivity; and moreover the vaccine has a strong immunity specificity, can activate the immunized animals to generate enough immunity antibodies, and generates a long-term protection. An MEV virus-like particle is produced according to the preparation method, the titer of virus liquid generated by a bio-reactor with a volume of 1000 L can reach a hemagglutination titer of 1:4096 to 1:32768; the expression quantity of granule protein is 65 mg / L, and reach liter of virus liquid semi-finished product can produce 3000 to 8000 doses of standard mink vaccine. The recombinant subunit vaccine is a novel vaccine having a prominently better effect than that of conventional inactivated tissue vaccines.
Owner:QILU ANIMAL HEALTH PROD
Virus pesticide suspending agent containing spinosad
InactiveCN104522055AGuaranteed stabilityEasy to storeBiocideAnimal repellantsSpinosadNuclear Polyhedrosis Virus
The invention discloses a virus pesticide suspending agent containing spinosad. The virus pesticide suspending agent is prepared from the following components in percentage by weight: 0.1%-20% of spinosad, 1%-10% of a dispersing agent, 1%-7% of an antifreezing agent, 0.2%-10% of a stabilizer, 0.1%-5% of a synergist, 0.5%-2% of an ultraviolet protecting agent, 0.1%-2% of an antifoaming agent, 0.1%-3% of a pH modifier and the balance of water; the prepared suspending agent also absorbs an insect virus; the insect virus is one or combination of two of a nuclear polyhedrosis virus and a granulosis virus NPV; the content of an insect virus inclusion body in the suspending agent is 5-20,000,000,000 PIB / ml; and the spinosad is one or a mixture of two of spinosad and ethyl spinosad. The spinosad and a virus pesticide disclosed by the invention are compounded, so that the problem of insect resistance can be solved, and the virus pesticide suspending agent is environment-friendly and safe.
Owner:JIANGXI NEW DRAGON BIOTECH
Ectropis grisescens warreh nuclear polyhedrosis virus bakthane insect disinfestation suspension agent and preparation method thereof
Owner:WUHAN UNIOASIS BIOLOGICAL TECH CO LTD
Special formula fertilizer for common head cabbage and preparation method thereof
InactiveCN108358695AGood formulaGood formula ratioAlkali orthophosphate fertiliserAmmonium orthophosphate fertilisersPhosphatePolyhedral virus
The invention belongs to the technical field of production of agricultural fertilizer and relates to special formula fertilizer for common head cabbage. The special formula fertilizer is prepared fromthe following raw materials in parts by weight: 60 to 80 parts of amino acid, 60 to 80 parts of humic acid, 5 to 10 parts of insect nuclear polyhedrosis virus, 40 to 60 parts of soybean meal, 208 to255 parts of urea, 108 to 174 parts of industrial-grade monoammonium phosphate, 80 to 110 parts of potassium sulfate and 0.5 to 1 part of compound microorganism bacterium liquid, wherein in the fertilizer, the content of organic matters is 20 percent to 40 percent, the sum of N, P and K is 19 percent to 40 percent and the content of compound microorganism bacteria is greater than or equal to 0.02billion / g. The invention further provides a preparation method of the special formula fertilizer for the common head cabbage; the preparation method comprises the following steps: determining physiochemical properties of soil of a planting land and calculating needed supplementation amounts of the N, P and K of the common head cabbage according to a fertilizer needing principle of a growth periodof the common head cabbage; crushing raw materials except the compound microorganism bacterium liquid and the soybean meal respectively and mixing to prepare fertilizer grains; after uniformly mixinga prepared compound microorganism bacterium agent and film-coating liquid, spraying on the surfaces of the fertilizer grains. By adopting the special formula fertilizer for the common head cabbage, the cost can be reduced, the yield is increased and the quality is improved.
Owner:WUHAN CHUQIANG BIOLOGICAL TECH CO LTD
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