781results about "Viruses/bacteriophages" patented technology

The present invention relates to the field of lentiviral delivery. Specifically provided are a mutated vesicular virus envelope protein and the use thereof in lentiviral vector packaging.

The invention discloses a broad-spectrum lytic bacteriophage for preventing and controlling various vibrio infections in an aquaculture environment. The invention relates to V.alginolyticus phage vBValM-R37J, which is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on August 6, 2025, the address is No.100 Courtyard, Xianlie Middle Road, Xianlie District, Guangzhou City, Guangdong Province, the postcode is 510070, and the preservation number is GDMCC NO.66813-B1. The invention further discloses a method for preparing the V.alginolyticus phage vBValM-R37J strain. The bacteriophage R37J is a candidate therapeutic bacteriophage with wide host spectrum, high splitting efficiency, gene safety and environmental stability, and has the potential of being popularized and applied in aquaculture as a novel biological tool for accurately preventing and controlling various vibrio infections. A new biological resource is provided for accurate prevention and control of aquatic pathogenic bacteria, and a theoretical basis and a technical support are provided for popularization and application of a phage therapy in an actual culture system.

The invention relates to the technical field of microorganisms, in particular to nocardia seriolae bacteriophage as well as a composition and application thereof. The name of the nocardia seriolae bacteriophage is RDP-NS002, the nocardia seriolae bacteriophage is preserved in the China General Microbiological Culture Collection Center on August 25, 2025, and the preservation number of the nocardia seriolae bacteriophage is CGMCC No.46674. The nocardia seriolae bacteriophage is named as RDP-NS002, and the nocardia seriolae bacteriophage is preserved in the China General Microbiological Culture Collection Center on August 25, 2025. The composition comprises the bacteriophage. The bacteriophage or the composition is prepared in the following steps: (1) preparing a product for killing nocardia seriolae; (2) preparing a product for inhibiting nocardia seriola; and (3) application in preparation of a product for preventing and / or treating fish nocardia disease caused by nocardia seriolae. The phage has the characteristics of high thermal stability, high acid-base stability and high splitting rate, has a long-acting inhibition effect, and is beneficial to prevention and treatment of nocardia seriola diseases.

The present application relates to a virus of reverse genetic operation construction of NADC34-like PRRSV2. Specifically, the full gene sequence of NADC34-like PRRSV2 BJ1805-2 isolated strain is segmented and connected to the vector by using PCR amplification, enzyme digestion and other methods with pACY177 plasmid as the carrier, and the BJ1805-2 full-length infectious clone recombinant plasmid (rBJ1805-2) is obtained. The infectious clone virus rBJ1805-2 is rescued in vitro, and the first NADC34-like PRRSV2 strain infectious clone platform in China is successfully built. The present application also relates to a modification method for constructing NADC34-like PRRSV2 strain which can adapt to Marc-145 cell passage culture. The infectious clone virus constructed by the present application has good in vitro and in vivo proliferation efficiency, and can cause specific viremia after inoculation in pigs; it has good safety, does not cause fever after inoculation in pigs, and will not cause piglet death. The modified strain can be used as a candidate strain for developing the first NADC34-like PRRSV2 specific vaccine in China, and is conducive to the prevention and control of PRRSV epidemic in China.

The invention relates to the technical field of stem cells, in particular to a lentivirus envelope plasmid combination and application thereof, a lentivirus and a packaging method thereof and a method for transduction of hematopoietic stem cells. The invention provides an envelope plasmid combination for lentivirus packaging, which is composed of lentivirus packaging plasmids containing VSVG glycoprotein and lentivirus packaging plasmids containing BaEV glycoprotein in a ratio of 3: 7. The invention further provides a method for transduction of the hematopoietic stem cells by the lentivirus, the method is simple and convenient to operate, the long-term dryness of the hematopoietic stem cells in vitro can be maintained, efficient and stable transduction of the hematopoietic stem cells can be realized, and the transduction rate is greater than 90%. The lentivirus transduction method hematopoietic stem cells can be used for hematopoietic stem cell gene therapy hematopoietic system genetic diseases, such as severe combined immunodeficiency, beta-thalassemia and sickle cell disease, Wiskott-Aldrich syndrome and the like, and the application prospect is good.

The invention belongs to the technical field of microorganisms, and discloses a high-temperature-resistant vibrio alginolyticus bacteriophage and a composition and application thereof, the high-temperature-resistant vibrio alginolyticus bacteriophage is named as vibrio alginolyticus bacteriophage PJ644 and is preserved in China General Microbiological Culture Collection Center on August 16, 2024, the preservation address is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.9624. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No. The bacteriophage has excellent high temperature resistance, and the bacteriophage and the bacteriophage composition not only can effectively prevent and treat various vibrio infections, but also can be used as active ingredients for preparing bacteriophage pharmaceutical preparations, aquatic feed additives, water disinfectants and aquatic product preservatives, and are used for effectively preventing and treating diseases caused by vibrio infections in aquaculture farms. The use is safe, and the problems of antibiotic residues and pathogen drug resistance caused by use of antibiotics are effectively avoided.

The invention discloses two mycobacterium bacteriophages and application thereof. The two bacteriophages both have a splitting spectrum width and efficient splitting capability, can specifically realize effective splitting of mycobacteria of five specific sequence types, and are further applied to the field of prevention and control of mycobacteria. Specifically, the bacteriophage MP2435 and the bacteriophage MP2451 not only can effectively inhibit the proliferation process of mycobacteria and block the formation of a biofilm of the mycobacteria, but also can efficiently kill the mycobacteria in a milk matrix; meanwhile, the two bacteriophages can still keep relatively high activity under the conditions that the temperature is 37-65 DEG C and the pH value is 3-12, and the bactericidal activity of the bacteriophages can be stably maintained even in acid-base, high-temperature and ultraviolet-containing extreme environments. Through the characteristics, the application scene and application range of the bacteriophage in mycobacterium prevention and control are remarkably widened, and an efficient and stable novel technical means is provided for biological prevention and control of mycobacterium.

The invention discloses a vibrio cantoniensis bacteriophage vBVcaSR45Z and an application of the vibrio cantoniensis bacteriophage The invention relates to Vibrio campbelli phage vBVcaSR45Z, which is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on December 8, 2025, the address is Courtyard 100 #, Xianlie Middle Road, Xiaolian District, Guangzhou City, Guangdong Province, the postcode is 510070, and the preservation number is GDMCC NO. 67441-B1. The invention further discloses a preparation method of the Vibrio campbelli phage vBVcaSR45Z strain. The bacteriophage vBVcaSR45Z has the advantages of rapid splitting capacity, high host inhibition effect, safe genome structure and clear systematic classification status, and provides an important basis for the bacteriophage vBVcaSR45Z as a biological prevention and control factor of aquatic vibrio pathogens.

The invention discloses a production process for culturing nuclear polyhedrosis virus by using an S2 or SF9 cell line. The preparation method comprises the following steps: connecting a cell-penetrating peptide C105Y with DSPE-PEG2000-Mal on the surface of a lipid nanoparticle, then preparing a lipid nanoparticle LNP-C105Y by using the DSPE-PEG2000-Mal, and entrapping nuclear polyhedrosis virus DNA to obtain DNAHcNPV LNP-C105Y; the DNAHcNPV coated LNP-C105Y is inoculated into an SF9 grassland spodoptera frugiperda cell line or an S2 fruit fly cell line to be cultured, and the nuclear polyhedrosis virus is produced. On the basis of the fall webworm nuclear polyhedrosis virus, DNAHcNPV-LNP-C105Y lipid nanoparticles are synthesized and prepared by adopting a film dispersion-extrusion method to wrap the fall webworm nuclear polyhedrosis virus, and the fall webworm nuclear polyhedrosis virus is produced by inoculating the fall webworm nuclear polyhedrosis virus to SF9 and S2 cell lines. The hyphantria cunea nuclear polyhedrosis virus production process is improved, and the defect that the hyphantria cunea nuclear polyhedrosis virus production process in the prior art cannot be separated from hyphantria cunea living body feeding is overcome.

The invention discloses a method for quickly rescuing bovine coronavirus epidemic strains and application of the method. The bovine coronavirus SHZ isolate is subjected to efficient segmented cloning, the obtained segment and a Linker sequence are subjected to a cyclic polymerase extension reaction, and the obtained reaction product can be directly transfected to 293T cells for virus packaging without purification. Different from a traditional method, the method does not need to amplify full-length virus cDNA clone by means of bacteria and yeast, but directly obtains a sufficient amount of preliminary products through PCR, and avoids the problems of instability and low efficiency when partial sequences of a virus genome proliferate in a bacteria or yeast host. By adopting the reverse genetic system, the recombinant bovine coronavirus expressing the foreign protein is successfully rescued, a brand new technical platform is provided for dynamic visualization research of in-vivo and in-vitro replication of the virus, and an efficient and flexible tool is also provided for virology research, development of virus vector vaccines and screening of antiviral drugs.

The invention provides an aeromonas hydrophila bacteriophage vBAhM7 and application thereof, and belongs to the technical field of microorganisms. According to the invention, aeromonas hydrophila is taken as host bacteria, a bacteriophage vBAhM7 is separated from sewage at a water inlet of a sewage treatment plant, and the titer can reach 109 PFU / mL; the tolerable temperature is 30-70 DEG C, and the high-temperature-resistant property is good; when the pH value is 3-12, the activity is relatively high, and the acid-base tolerance is good; the bacteriophage is not sensitive to chloroform, has no lipid coating on the shell, is stable in structure, and can resist the damage of organic solvents; the inhibition rate range on the host bacteria biofilm is 36.0%-68.6%, and good host bacteria biofilm inhibition capability is achieved; the in-vitro sterilization effect on host bacteria is good, and a relatively strong inhibition effect is achieved; the zebra fish can be protected; the antibacterial agent can be used for preventing and controlling aeromonas hydrophila in aquatic products, and the use of antibacterial agents in aquaculture is avoided or reduced.

The invention belongs to the technical field of microorganisms, and discloses a riemerella anatipestifer bacteriophage PT56, a bacteriophage composition thereof and application of the riemerella anatipestifer bacteriophage PT56, the collection number of the riemerella anatipestifer bacteriophage PT56 is CGMCCNO.46492, and the riemerella anatipestifer bacteriophage PT56 is collected in China General Microbiological Culture Collection Center on May 26, 2025, and the collection number is CGMCCNO.46492. The preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The riemerella anatipestifer bacteriophage PT56 has broad-spectrum bactericidal ability on riemerella anatipestifer of different serotypes, has certain temperature stability and acid-base stability, and can be used as an active ingredient for preparing drugs, disinfectants, kits and the like for preventing and treating riemerella anatipestifer diseases. The riemerella anatipestifer disinfection solution is widely applied to poultry diseases caused by riemerella anatipestifer and disinfection and sterilization of each link in the product production and processing process, is safe to use, and effectively reduces the incidence rate and the death rate of riemerella anatipestifer diseases such as poultry infectious serositis and the like.

The invention relates to the technical field of microorganisms, in particular to a citrobacter welchii bacteriophage as well as a composition and application thereof. The name of the citrobacter werkmanii bacteriophage is RDP-CI25012, the citrobacter werkmanii bacteriophage is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on August 25, 2025, and the preservation number of the citrobacter werkmanii bacteriophage is CGMCC (China General Microbiological Culture Collection Center) No. 46668. The composition comprises the bacteriophage. The bacteriophage or the composition is prepared in the following steps: (1) preparing a product for killing citrobacter welchii; (2) preparing a product for inhibiting citrobacter welchii; and (3) application in preparation of a product for preventing and / or treating citrobacter welchii disease caused by citrobacter welchii. The cracking rate of the phage is 80%, and the phage has an extremely high host range and a relatively wide cracking spectrum. After the bacteriophage is treated at 50 DEG C for 4 hours, the titer is still kept at 107 PFU / mL or above, and the bacteriophage has excellent thermal stability, so that a solid foundation is laid for use, storage, transportation and production processing of the bacteriophage in most practical application scenes.

The invention relates to a ralstonia solanacearum bacteriophage LC10 capable of preventing and controlling tobacco bacterial wilt, the classification name of the ralstonia solanacearum bacteriophage LC10 is Rastonia phage, the preservation number of the ralstonia solanacearum bacteriophage LC10 is GDMCC No: 66716-B1, and the preservation date of the ralstonia solanacearum bacteriophage LC10 is July 21, 2025. The bacteriophage is a brachytail bacteriophage and can specifically split ralstonia solanacearum, inhibit the growth of the ralstonia solanacearum and enhance the disease inhibition capability of soil. Therefore, the bacteriophage LC10 can be used for preparing related preparations for preventing and controlling tobacco bacterial wilt.

The invention discloses a recombinant M13 bacteriophage, a K88 targeting antibacterial preparation containing the bacteriophage and application of the K88 targeting antibacterial preparation, and belongs to the field of biotechnology and veterinary medicine. The antibacterial preparation comprises a recombinant M13 bacteriophage rM13; a K88 targeting element is displayed on a tail filament pIII protein of the recombinant M13 bacteriophage, and the K88 targeting element is a nano antibody NBK88 of which the amino acid sequence is as shown in SEQ ID NO: 1. Through engineering modification, the rM13 can specifically recognize, infect and split enterotoxigenic escherichia coli expressing K88 fimbriae, so that the host range of the wild type M13 is accurately guided to pathogenic bacteria. The invention also provides application of the preparation in preparation of medicines for preventing or treating ETEC infection, and the dosage form of the preparation is preferably a freeze-dried preparation or an enteric-coated preparation capable of being released in a targeted intestinal tract. The preparation provided by the invention has the remarkable advantages of strong targeting property, self-proliferation, no damage to intestinal microecology and capability of overcoming bacterial drug resistance.

The invention discloses a mycobacteriophage and application thereof, and relates to the technical field of bacteriophages. The mycobacteriophage is named as Mycobacter phage BZNK001, is preserved in Guangdong Microbial Culture Collection Center, has a preservation number of GDMCC No: 67907-B1, does not contain antibiotic drug-resistant genes and integrase genes, has excellent biological safety and good environmental tolerance, can maintain high biological activity in a temperature range of 37-50 DEG C under an acid-base condition of pH 5-9, and can be used for preparing the antibiotic drug-resistant gene and integrase gene-containing mycobacteriophage for preventing and treating the antibiotic drug-resistant gene and integrase gene-containing mycobacteriophage for preventing and treating the antibiotic drug-resistant gene and integrase gene-containing mycobacteriophage. The bacillus subtilis has broad-spectrum and high-efficiency non-tuberculous mycobacterium cracking activity, and can effectively crack four clinical key mycobacteria such as mycobacterium abscessus, mycobacterium avium, mycobacterium intracellularis and mycobacterium smegmatis. According to the invention, a mycobacteriophage resource library is enriched, a novel and stable biological prevention and control means is provided for efficient prevention and control of mycobacteria, and the mycobacteriophage has good clinical application and industrialization values.

The invention belongs to the technical field of bacteriophages, and discloses an edwardsiella bacteriophage, a bacteriophage composition and application thereof, the bacteriophage is named as edwardsiella bacteriophage PREE 50, the bacteriophage is preserved in China General Microbiological Culture Collection Center on May 26, 2025, and the preservation number of the bacteriophage is CGMCC NO. The preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.46488. The bacteriophage PREE 50 is wide in lysis spectrum, high in lysis rate for various types of edwardsiella, good in acid-base stability and temperature stability and capable of being used for preparing drugs, water disinfectants, aquatic feed additives, detection kits and the like for treating or preventing edwardsiella infection, and the bacteriophage PREE 50 can be applied to preparation of drugs, water disinfectants, aquatic feed additives, detection kits and the like while solving edwardsiella infection. And the problems of antibiotic residues and pathogen drug resistance caused by the use of antibiotics are avoided.

The invention discloses a cat kidney suspension culture cell line suitable for serum-free suspension culture and application of the cat kidney suspension culture cell line. The name of the cell line is F81-B4, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.46350. After 50 generations of continuous subculture, the cells still can maintain the original proliferation level and cellular morphology, the cell number can reach 9.0 * 10 < 6 > / mL after the cells are subcultured at the density of 1.0 * 10 < 6 > / mL for three days, the multiplication time is 22-26 hours, and the cell proliferation speed is equivalent to that in a shake flask when the cells are amplified to a 10L bioreactor for culture. The cell line is highly susceptible to feline parvovirus, feline calicivirus, feline herpes virus, canine parvovirus and canine distemper virus, the virus content of a virus solution harvested 72 h after FPV virus inoculation can reach 107.5 TCID50 / mL, the virus content of a virus solution harvested 24 h after FCV virus inoculation can reach 1010.8 TCID50 / mL, the virus content of a virus solution harvested 48 h after FHV-1 virus inoculation can reach 107.5 TCID50 / mL, compared with an existing F81 adherent cell production process, the production process has the advantages that the production efficiency is high, and the production cost is low. The method has the characteristics of rapid lesion, short virus collection time and high titer. The cell matrix is an ideal cell matrix which can be used for culturing the three viruses.

The invention relates to an application of a CAR exosome drug carrier in tumor treatment. The application of the CAR exosome loaded medicine in tumor treatment is based on the strategy of CAR exosome coupling medicine, so that the completeness of the exosome and the controllability of the medicine loading capacity are greatly protected, the purpose of accurately delivering the medicine to tumors in a targeted manner is achieved, and a new direction is provided for tumor treatment.

The invention belongs to the field of biological prevention and control, and discloses an insect specific virus NLBV3 and application thereof in reducing transmission of rice odontodwarf virus of brown planthopper. The NLBV3 virus genome comprises three chains, the L chain encodes RNA dependent RNA polymerase (RdRP), the M chain encodes glycoprotein precursor (GP), the S chain encodes nucleoprotein (NP), and the nucleotide sequences are respectively shown as SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3; the application is realized by releasing the NLBV3-carrying brown planthopper population, and according to the screening and detection method of the NLBV3-carrying brown planthopper high-virus population provided by the invention, natural brown planthoppers can be effectively infected after the NLBV3-carrying brown planthopper high-virus population is released, the RRSV transmission capability of the brown planthoppers is reduced, and the virus is prevented from damaging rice production. The NLBV3 provided by the invention is an insect specific virus, is harmless to mammals, fishes and shrimps, natural enemy insects and pollination insects, and has the advantages of specific action object, environment friendliness and the like.

Provided herein are methods and systems for use in determining risk of a Staphylococcus infection, predicting efficacy of a Staphylococcus vaccine, and prognosing clinical outcomes on a subject exposed to Staphylococcus. Methods of treating subject identified as having or at risk of a Staphylococcus infection are further provided.

The present disclosure relates to three-dimensional nucleic acid origami nanostructures that are designed to allow for self-assembly of the nanostructures into a larger structure (e.g., cylindrical, icosahedral, etc.) about the surface of a virus particle, and their use in treatment methods.

The invention relates to the field of biological medicine, and particularly discloses a regulatory element combination for driving nucleic acid expression based on ITR-enhancer and application of the regulatory element combination. The regulatory element combination for driving nucleic acid expression based on an ITR-enhancer comprises at least one inverted terminal repeat (ITR) and at least one enhancer, the regulatory element combination is delivered through an AAV vector. The invention also provides a recombinant nucleic acid molecule for improving specific delivery of the target gene. The recombinant nucleic acid molecule comprises the regulatory element combination, an exogenous target gene and polyadenylic acid. The AAV vector provided by the invention can be used for driving the expression of a target gene without the action of a traditional promoter, and has the advantages of enhancer tissue specificity, short sequence (50-100bp), pathological microenvironment response and the like, thereby breaking through the capacity, targeting and dynamic limitation of the traditional AAV vector, improving the DNA (Deoxyribose Nucleic Acid) carrying capacity of the AAV vector, and simultaneously improving the expression of the target gene. The tissue and spatio-temporal expression specificity of a target gene is realized.

The invention discloses an FAM20B protein and application of an FAM20B protein inhibitor in prevention or treatment of glucolipid metabolism related diseases. Specifically, the invention discloses an application of an FAM20B protein and / or an FAM20B gene in regulation and control of liver glycolipid metabolism. The invention also discloses application of the FAM20B inhibitor in improving, preventing or treating liver glycolipid metabolism disorder or glycolipid metabolism related diseases (such as diabetes, metabolism related fatty liver disease MAFLD, obesity and the like). Experiments show that the FAM20B inhibitor can reduce the liver gluconeogenesis level, relieve impairment of glucose tolerance, relieve hyperglycemia of diabetic patients, reduce the fat content of the whole body and the liver, relieve liver lipid deposition, promote energy consumption, reduce food intake, promote fat burning and reduce liver fat accumulation; the compound can be used for preparing medicines for preventing and treating glucose and lipid metabolism disorder or glucose and lipid metabolism related diseases, and has a wide application prospect in the fields of prevention and treatment of metabolism related diseases.

The invention belongs to the field of veterinary biological products, and relates to a porcine reproductive and respiratory syndrome virus strain and application thereof. The invention particularly discloses a porcine reproductive and respiratory syndrome virus strain with a microbial preservation number of CGMCC (China General Microbiological Culture Collection Center) No.47095. The invention also discloses a vaccine composition prepared from the strain. After pigs are inoculated with the vaccine composition, the vaccine composition can resist challenge of a porcine reproductive and respiratory integrated virus SD strain and a porcine reproductive and respiratory integrated virus HuN4 strain, and has application prospects.

The invention discloses a pasteurella phage PTP1APTYT15 as well as a composition, a kit and application of the pasteurella phage PTP1APTYT15, the pasteurella phage PTP1APTYT15 is preserved in Guangdong Microbial Culture Collection Center on September 10, 2025, the preservation number of the pasteurella phage PTP1APTYT15 is GDMCC NO: 66946-B1, and the pasteurella phage PTP1APTYT15 is classified and named as Pasteurella phage PTP1APTYT15. The pasteurella phage PTP1APTYT15 provided by the invention has the characteristics of high temperature resistance and alkali resistance, and can be used for cracking pasteurella of various serotypes. In addition, the bacteriophage is high in proliferation efficiency and high in splitting capacity, has no adverse effect on human or animal health, and has good biological safety.

The invention provides an anti-PD-1 antibody, a CAR-T cell, and a preparation method and application thereof. The anti-PD-1 antibody comprises LCDR-1-3 as shown in SEQ ID NO: 1-3 and HCDR-1-3 as shown in SEQ ID NO: 4-6, respectively. The CAR-T cell expresses a novel element for efficiently blocking the PD-1, and the element is a single-chain antibody for targeting the PD-1 in a cell membrane anchoring manner. And the chimeric antigen receptor and the cell membrane anchored anti-PD-1 scFv are connected by a 2A cleavage protein. The membrane anchor type anti-PD-1 scFv expressed by the CAR-T cell can almost completely block the expression of PD-1 on the surface of a T cell membrane, and further block a signal channel combined with PD-1 / PD-L1, so that the capability of T cell depletion caused by antagonism tumor of the CAR-T cell is enhanced, and the purpose of enhancing the anti-tumor effect of the CAR-T cell is achieved.

The application discloses a kind of multi-modal biological aerosol simulation materials based on high stability fluorescent protein label and construction method thereof, belong to biological aerosol simulation technical field.The simulation material is made of the microorganism expressing StayGold fluorescent protein by genetic engineering modification, and is compounded with the hydrophobic silicon dioxide particles of specific particle size.The core of the present application is: first use the StayGold protein with excellent light stability as biomarker, overcome the defect that traditional fluorescent protein is easily quenched in simulation environment;Innovatively design the "microorganism-dust" composite particle structure, by optimizing the concentration of bacterial suspension, particle ratio and adding isopropyl alcohol additive, generate aerosol with particle size distribution of 1-10 μm by atomization technology, highly reproduce the physical and chemical state of biological aerosol in real environment.The simulation material maintains high survival rate and fluorescent signal stability in wide temperature range and wide humidity range.

In order to control plant diseases caused by Xanthomonas bacteria, a novel bacteriophage exhibiting bacteriolytic activity specific to the same bacteria is isolated, and a plant disease control composition containing the same as an active ingredient is developed and provided. Provided are a lysozyme containing a bacteriophage having a novel genomic DNA sequence and exhibiting a lysolytic activity specific to Xanthomonas bacteria, and a plant disease control composition containing the lysozyme as an active ingredient.

The invention discloses a novel acinetobacter baumannii phage vBAbaMpha0264 and application thereof, and belongs to the technical field of microorganisms. The acinetobacter baumannii phage vBAbaMpha0264 is preserved in the Guangdong Microbiological Culture Collection Center on August 28, 2025, the preservation number of the acinetobacter baumannii phage vBAbaMpha0264 is GDMCC (China General Microbiological Culture Collection Center) NO: 66893-B1, and the preservation address of the acinetobacter baumannii phage vBAbaMpha0264 is Institute of Microbiology, Academy of Sciences, Guangdong. The bacteriophage vBAbaMpha0264 has high cracking activity, environmental stability and safety, shows a breakthrough host range, and can be used for effectively cracking a plurality of acinetobacter bacteria including hospital acinetobacter and acinetobacter sysei. Meanwhile, the bacteriophage has excellent chloroform tolerance, and the stability of the bacteriophage is obviously superior to that of the existing bacteriophage. Due to the characteristics, the phage vBAbaMpha0264 overcomes the technical prejudice of'narrow spectrum and instability 'of the traditional phage, and a brand new technical scheme is provided for developing a universal antibacterial preparation covering more pathogenic bacteria.