246 results about "Vibrion" patented technology

The invention discloses a virulent bacteriophage vBValSR36H with a host being vibrio alginolyticus and application of the virulent bacteriophage vBValSR36H, and relates to the technical field of microbial control. The invention provides a bacteriophage capable of efficiently cracking Vibrio alginolyticus ATCC 17749T, the bacteriophage is named as vBValSR36H, the preservation number is 66248-B1, and the preservation address is Institute of Microbiology, Academy of Sciences in Guangdong Province. The vibrio alginolyticus bacteriophage vBValSR36H provided by the invention has good environmental stability and certain bactericidal activity, and has a relatively wide environmental adaptation range and relatively long survival time. The bacteriophage vBValSR36H provided by the invention can specifically and effectively kill, prevent and control related vibrio diseases, meanwhile, virulence factor genes and drug-resistant genes are not detected in the bacteriophage, and the bacteriophage vBValSR36H has the advantages of no toxic or side effect, high safety, environmental friendliness and wide application prospect. Therefore, the vibrio alginolyticus bacteriophage vBValSR36H can be completely applied to killing and / or preventing vibrio alginolyticus for non-disease diagnosis and treatment purposes.

The invention discloses a broad-spectrum lytic bacteriophage for preventing and controlling various vibrio infections in an aquaculture environment. The invention relates to V.alginolyticus phage vBValM-R37J, which is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on August 6, 2025, the address is No.100 Courtyard, Xianlie Middle Road, Xianlie District, Guangzhou City, Guangdong Province, the postcode is 510070, and the preservation number is GDMCC NO.66813-B1. The invention further discloses a method for preparing the V.alginolyticus phage vBValM-R37J strain. The bacteriophage R37J is a candidate therapeutic bacteriophage with wide host spectrum, high splitting efficiency, gene safety and environmental stability, and has the potential of being popularized and applied in aquaculture as a novel biological tool for accurately preventing and controlling various vibrio infections. A new biological resource is provided for accurate prevention and control of aquatic pathogenic bacteria, and a theoretical basis and a technical support are provided for popularization and application of a phage therapy in an actual culture system.

Provided are a method for controlling a soilborne disease in which a load on an environment and a human body is reduced, and a novel predatory bacterium therefor.　Provided are: a predatory bacterium or a variant thereof selected from the group consisting of bacteria of the genus Bacteriovorax and bacteria of the genus Bdellovibrio which selectively prey on a pathogenic bacterium belonging to the family Burkholderiaceae, the family Pectobacteriaceae, or the family Lysobacteraceae; a method for controlling a soilborne plant disease caused by the pathogenic bacterium, the method characterized in that the predatory bacterium or variant thereof is applied to a seed, root, or underground stem of a plant, rhizosphere soil, or soil; and a composition.

The invention discloses a concrete crack self-repairing material based on microorganism immobilization and a preparation method of the concrete crack self-repairing material, and belongs to the crossing field of microorganism materials and civil engineering. The material is prepared from peptone, sodium lactate, volcanic rock, bacillus pasteurii and other raw materials through the steps of culture medium preparation, carrier modification, microorganism immobilization, microcapsule and gel protection layer formation and the like, the prepared volcanic rock-chitosan carrier provides an inhabiting environment for microorganisms, and the survival rate of the microorganisms in a high-alkali environment is increased to 70%. When the concrete cracks and the alkaline environment triggers the protective layer to disintegrate, the bacillus pasteurii and the desulfurization vibrio are subjected to a reduction reaction with sulfate through urealysis, and calcium carbonate and calcium sulfide are generated to fill cracks. The 28-day repair rate of the material is larger than or equal to 99%, the maximum crack repair width reaches 1.2 mm, the compressive strength recovery rate is larger than or equal to 85%, excellent durability and environment friendliness are achieved, and the concrete crack repair problem is effectively solved.

The invention provides a lactobacillus pentosus and a preparation method and application of bacteriocin of the lactobacillus pentosus. The name of the strain is lactobacillus pentosus YCL31, and the strain is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on June 25, 2025, the preservation address is the 5th floor of the building 59, No. 100 courtyard, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCC No: 66589. The bacteriocin separated and purified from the metabolite of the bacterium can destroy the morphological structure of the vibrio parahemolyticus and inhibit the toxicity of the vibrio parahemolyticus, can also achieve the effect of remarkably inactivating the vibrio parahemolyticus in a metapenaeus ensis system, can effectively delay the spoilage of the metapenaeus ensis, and has a good application prospect as a bacteriostatic agent or preservative. Therefore, development and application of the novel bacteriocin have important reference value in related fields.

The invention discloses a marine streptomyces strain SHEa3, a compound derived from the marine streptomyces strain SHEa3 and application of the marine streptomyces strain SHEa3, the classification name of the marine streptomyces strain SHEa3 is Streptomyces sp, the preservation number of the marine streptomyces strain SHEa3 is GDMCC No: 66667, and the preservation date of the marine streptomyces strain SHEa3 is July 14, 2025. The invention also discloses a sesquiterpenoids compound streptaviol A derived from marine streptomyces and application of the sesquiterpenoids compound streptaviol A in preparation of drugs for preventing and treating vibrio diseases of mariculture animals. The invention further discloses application of the sesquiterpenoids, namely the sea threitol C, derived from the marine streptomyces in preparation of drugs for preventing and treating vibrio diseases of mariculture animals. The marine streptomyces source compound has efficient anti-vibrio activity, and the marine streptomyces source compound streptaviol A and the helithreitol C are used for preparing the medicine for preventing and treating vibrio diseases of mariculture animals, so that the side effect is low, and the activity is excellent.

The invention belongs to the technical field of microorganisms, and discloses a high-temperature-resistant vibrio alginolyticus bacteriophage and a composition and application thereof, the high-temperature-resistant vibrio alginolyticus bacteriophage is named as vibrio alginolyticus bacteriophage PJ644 and is preserved in China General Microbiological Culture Collection Center on August 16, 2024, the preservation address is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.9624. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No. The bacteriophage has excellent high temperature resistance, and the bacteriophage and the bacteriophage composition not only can effectively prevent and treat various vibrio infections, but also can be used as active ingredients for preparing bacteriophage pharmaceutical preparations, aquatic feed additives, water disinfectants and aquatic product preservatives, and are used for effectively preventing and treating diseases caused by vibrio infections in aquaculture farms. The use is safe, and the problems of antibiotic residues and pathogen drug resistance caused by use of antibiotics are effectively avoided.

The invention discloses a vibrio cantoniensis bacteriophage vBVcaSR45Z and an application of the vibrio cantoniensis bacteriophage The invention relates to Vibrio campbelli phage vBVcaSR45Z, which is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on December 8, 2025, the address is Courtyard 100 #, Xianlie Middle Road, Xiaolian District, Guangzhou City, Guangdong Province, the postcode is 510070, and the preservation number is GDMCC NO. 67441-B1. The invention further discloses a preparation method of the Vibrio campbelli phage vBVcaSR45Z strain. The bacteriophage vBVcaSR45Z has the advantages of rapid splitting capacity, high host inhibition effect, safe genome structure and clear systematic classification status, and provides an important basis for the bacteriophage vBVcaSR45Z as a biological prevention and control factor of aquatic vibrio pathogens.

The invention discloses bacillus orientalis ZL3 for regulating rhizosphere soil microecosystems of haloxylon ammodendron soil in deserts and promoting the growth of the haloxylon ammodendron soil, particularly relates to a fungicide ZL3 for promoting the growth of haloxylon ammodendron and regulating rhizosphere microbial communities and application of the fungicide ZL3, and belongs to the technical field of microorganisms. According to the bacillus toonensis ZL3 provided by the invention, the conversion of soil nutrients to available nutrients is improved, and the rhizosphere microbial community structure is improved, so that the effect of promoting the growth of haloxylon ammodendron is achieved. When the ZL3 is applied to a haloxylon ammodendron field, the content of soil organic matters, the content of soil quick-acting potassium, the content of available phosphorus and the activity of sucrase are remarkably improved by 37.78%, 11.01%, 49.66% and 67.71% respectively. The plant height is increased by 17.59%, and the root length is increased by 109.45%. Meanwhile, the positive change of microbial communities is regulated and controlled, especially the enrichment of beneficial bacteria, the relative abundance of the prevotella and the vibrio is remarkably increased, and the relative abundance of the burkholderia is remarkably reduced. In general, inoculation of the ZL3 fungicide can promote the growth and development of haloxylon ammodendron by improving the soil fertility and improving the rhizosphere microflora structure.

The present application belongs to the field of aquaculture technology, and mainly relates to a traditional Chinese medicine composition for preventing and treating acute hepatopancreas necrosis disease of prawn, a preparation method and application. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 28-32 parts of Radix Sanguisorbae, 28-32 parts of Radix Scutellariae, 18-22 parts of Phellodendri Cortex and 8-12 parts of Lonicerae Japonicae Flos. The traditional Chinese medicine composition can significantly inhibit the activity of the main pathogen of the disease, i.e. Vibrio parahaemolyticus, improve the immune function and disease resistance of prawn, and further repair the hepatopancreas and intestinal tissues of the diseased prawn, thereby achieving the effect of effectively preventing and treating the acute hepatopancreas necrosis disease of prawn.

The invention belongs to the technical field of microbial antibacterial preparations, and particularly discloses a novel strong vibrio parahaemolyticus bacteriophage isolated strain vBVpSCC6 and application of the novel strong vibrio parahaemolyticus bacteriophage isolated strain vBVpSCC6. The bacteriophage vBVpSCC6 is preserved in the Guangdong Microbial Culture Collection Center on September 14, 2020, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 61189-B1. The bacteriophage has good tolerance to environments such as temperature, pH, ultraviolet rays and the like, and has a good prevention and control effect on aquatic product pollution caused by vibrio parahaemolyticus. The bacteriophage has a lysis effect on multi-drug-resistant vibrio parahaemolyticus, can replace antibiotics to play a bacteriostatic role, effectively solves the problem of drug resistance faced by current antibiotic treatment, and has relatively good utilization value and application prospect. The bacteriophage can also be prepared into a composition with other bacteriophages to play a synergistic effect, and the bacteriophage can be applied to wider-spectrum antibacterial or bacteriostatic application.

The invention discloses a nano antibody for detecting vibrio parahaemolyticus as well as a preparation method and application thereof, and aims to provide a nano antibody for detecting vibrio parahaemolyticus, which is high in detection accuracy, strong in specificity and simple in obtaining method. The amino acid sequence of the nano antibody is as shown in SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4 or SEQ ID NO. 5. Belongs to the technical field of biological detection.

The invention belongs to the field of prevention and control of bacterial diseases of aquatic animals, and discloses an acid-resistant broad-spectrum vibrio parahaemolyticus bacteriophage LVPP58 and application of the acid-resistant broad-spectrum vibrio parahaemolyticus bacteriophage LVPP58 in vibrio disease prevention and control, and the preservation number of the bacteriophage is CCTCC M 2025322. The bacteriophage is a broad-spectrum vibrio parahaemolyticus bacteriophage, not only can split vibrio parahaemolyticus, but also can split vibrio alginolyticus, the bacteriophage LVPP58 is stable in titer at the pH of 3-11 and the temperature of 30-60 DEG C, the bacteriophage and antibiotics are combined for use, a synergistic effect can be generated, the treatment effect is enhanced, the occurrence rate of drug resistance is reduced, and a new strategy is provided for solving the problem of bacterial drug resistance.

The invention belongs to the technical field of pathogenic microorganism screening, and particularly relates to vibrio parahaemolyticus capable of causing prawn vibrio vitreovibriosis and application of the vibrio parahaemolyticus, and the pathogenic bacteria can be used for preparing inactivated vaccines or used as the pathogenic bacteria to screen medicine components with the effect of preventing and treating the prawn vibrio vitreovibriosis or a culture method. The preservation number of the vibrio parahaemolyticus provided by the invention is CGMCC (China General Microbiological Culture Collection Center) The pathogenicity of the vibrio parahaemolyticus with high pathogenicity and strong lethality is about 1000 times stronger than that of the vibrio parahaemolyticus which is collected by the world animal hygiene organization and causes acute hepatopancreatic necrosis, and the strain is popular in main prawn culture areas and causes serious harm to the prawn fry culture industry in China. The vibrio parahaemolyticus provided by the invention can be used as a positive control for preparing a diagnosis product for the prawn vibrio vitreovirus disease; or preparing an immune vaccine after inactivation.

The invention discloses a composition for treating hyperuricemia nephropathy and application thereof, and belongs to the field of biological medicines.The composition is prepared from, by weight, 28-32 parts of rhizoma smilacis glabrae, 28-32 parts of yam rhizome, 13-17 parts of radix clematidis, 8-12 parts of peach kernels, 8-12 parts of flos carthami, 8-12 parts of herba lycopi, 18-22 parts of rhizoma alismatis, 28-32 parts of raw coix seeds, 28-32 parts of semen plantaginis, 13-17 parts of rhizoma atractylodis and 13-17 parts of pseudobulbus cremastrae seu pleiones. The composition can obviously reduce the uric acid level of a mouse with high uric acid nephropathy and improve kidney injury; meanwhile, intestinal flora of mice with the hyperuricemia nephropathy can be remarkably remodeled, abundance of beneficial bacteria Ackerman's bacteria is up-regulated, abundance of opportunistic pathogenic bacteria such as escherichia coli-shigella, desulfurization vibrio and enterococcus is down-regulated, and important significance is achieved for research and drug development of the hyperuricemia nephropathy.

The invention belongs to the technical field of microorganisms, and discloses a bifidobacterium animalis subsp. Lactis XB02 strain capable of inhibiting pathogenic bacteria and improving inflammation, the preservation number of the bifidobacterium animalis subsp. Lactis XB02 strain is CGMCC (China General Microbiological Culture Collection Center) No.34942, and the preservation date is June 18, 2025. Meanwhile, the probiotic composition adopting the bacterial strain XB02 also comprises a lactobacillus rhamnosus XB01 bacterial strain. The XB02 strain disclosed by the invention has excellent bacteriostatic activity, especially for vibrio parahaemolyticus and gardneria; the atopic dermatitis symptom can be obviously improved; the intestinal mucosa permeability is reduced, and the intestinal barrier function is enhanced; and when the strain is combined with the XB01 strain for use, a synergistic sleep promoting effect is shown. The invention further provides a probiotic composition containing the strain, a preparation and application of the probiotic composition and the preparation in food, medicine or health care products.

The invention belongs to the technical field of microorganism application, and particularly relates to a genetically engineered bacterium for producing L-alanine and application of the genetically engineered bacterium in two-stage fermentation of the L-alanine, and the genetically engineered bacterium takes vibrio natrievibrio as a chassis bacterium and can co-express an alanine dehydrogenase gene and an alanine transporter gene. An alanine dehydrogenase gene is heterologously introduced into a vibrio natriticus genome, and an inactivated gene combination with the optimal L-alanine yield is found out by randomly inactivating a key enzyme in a byproduct production pathway, so that the vibrio natriticus genetically engineered bacterium capable of efficiently producing the L-alanine through a fermentation method is constructed. According to the present invention, the L-alanine is produced through the two-stage fermentation by using the gene engineering bacteria through the temperature-sensitive control strategy so as to reduce the influence of the accumulation of the L-alanine on the cell growth, and the high production of the Xiabanmate is provided. The genetically engineered bacterium is short in growth cycle, glucose can be efficiently utilized to convert the genetically engineered bacterium into L-alanine, the L-alanine is transferred to the outside of cells, separation and purification are simple, cell disruption is not needed, and the yield and conversion rate of the L-alanine can both reach a high level.

The invention discloses a lactobacillus acidophilus FMM01 with the effects of improving the contractility of pelvic floor muscles and repairing mucous membranes and application of the lactobacillus acidophilus FMM01, the lactobacillus acidophilus FMM01 is preserved in the China Center for Type Culture Collection, the preservation date is January 9, 2024, and the preservation number is CCTCC NO: M2024052. The lactobacillus acidophilus FMM01 has the advantages of self-adaption, high acid resistance, strong mucous membrane adhesiveness and no irritation to vaginal mucosa. The lyophilized powder is high in activity and excellent in stability; experimental results show that the lactobacillus acidophilus FMM01 can effectively inhibit bacteria, and especially can effectively inhibit enterobacter cloacae, vibrio parahaemolyticus and / or candida albicans. Besides, the lactobacillus acidophilus FMM01 can improve vaginal relaxation, improve pelvic floor muscle contractility and / or recover vaginal mucosa functions, can promote recovery of healthy florae after mixed vaginitis antibiotic / hormone treatment, improves the treatment effect of mixed vaginitis, and remarkably reduces the recurrence rate of mixed vaginitis.

The application discloses a Vibrio harveyi oral vaccine and a preparation method and application thereof. The application uses a lactococcus lactis NZ9000 expression system, modifies transformation parameters, effectively expresses Vibrio harveyi OmpU protein, constructs a lactococcus lactis (NZ9000) engineering bacterium expressing Vibrio harveyi outer membrane protein (OmpU), develops an oral vaccine against Vibrio harveyi infection, aims to reduce the infection of economic animals in seawater aquaculture by oral administration, provides a new scheme for dealing with the harm of Vibrio harveyi infection and antibiotic resistance and the like, promotes the breeding of fry and fingerlings, and promotes the development of biological agriculture and related industries. The strategy is expected to overcome the problems of traditional injection vaccines, such as inconvenient operation and great stress, and can provide a new technical scheme for reducing the dependence of the aquaculture industry on antibiotics and promoting the establishment of a green and healthy breeding mode.

The invention provides a bacteriostatic agent containing an agilawood leaf extracting solution for inhibiting vibrio alginolyticus and application of the bacteriostatic agent, and relates to the field of plant extracts. The bacteriostatic agent disclosed by the invention is an agilawood leaf extracting solution; the agilawood leaf extracting solution is prepared by adding water into agilawood leaves, crushing and extracting, and the material-liquid ratio of the agilawood leaves to the water is 1g: (2-4mL); the agilawood leaf extracting solution is used for inhibiting vibrio alginolyticus. The preparation method of the agilawood leaf extracting solution comprises the following steps: taking agilawood leaves, adding water, crushing into homogenate, filtering to remove impurities, centrifugally separating, collecting supernate, and sterilizing to obtain the agilawood leaf extracting solution. The minimum inhibitory concentration of the agilawood leaf extracting solution prepared by the invention on vibrio alginolyticus is 15.63 mg / ml. The agilawood leaf extracting solution prepared by the invention can well inhibit vibrio alginolyticus, is used for preparing a bacteriostatic agent for inhibiting vibrio alginolyticus, avoids the risk of drug-resistant genes, has no risk of organic solvent residue, and has the advantages of high efficiency, risk reduction, cost reduction and the like.

The application provides a new bacteriophage for preventing and treating vibrio diseases of aquatic animals and application thereof, the bacteriophage is preserved in China Center for Type Culture Collection on September 4, 2020, and the preservation number is CCTCC M 2020465. The bacteriophage is a new species of bacteriophage of the Caudovirales class and Queuovirinae subfamily, the whole genome of the bacteriophage is OP331229.1 in the NCBI official website GeneBank. The bacteriophage has a wide phage spectrum, can crack 6 different species and genera of vibrio at the same time, can crack 45 strains of Vibrio parahaemolyticus, 22 strains of Vibrio harveyi, 17 strains of Vibrio alginolyticus, 13 strains of Vibrio vulnificus, 11 strains of Vibrio campbellii and 9 strains of Vibrio cholerae at the same time, can more effectively solve the problem of multiple bacterial disease infections in aquaculture, and can be used as a microecological preparation for preventing and treating vibrio diseases such as Vibrio parahaemolyticus, Vibrio harveyi and Vibrio alginolyticus of aquatic animals.

The application discloses a freeze-drying protective agent of Vibrio harveyi phage V-YDF132 and a preservation method thereof. The freeze-drying protective agent contains inulin, skimmed milk powder, gelatin, L-glutamic acid sodium and water. The freeze-drying protective agent is mixed with a phage V-YDF132 solution, and then the mixture is frozen to a solid state, and after being subjected to freeze-drying, a phage V-YDF132 freeze-drying powder preparation is obtained. There are various preservation methods for phages, and the best preservation method is different for different phages. The application screens and prepares different phage freeze-drying protective agents and explores the protection effect of the freeze-drying protective agents on phages after freeze-drying, so as to lay a foundation for phage preservation. The phage powder preparation prepared from the freeze-drying protective agent formula P9 and the phage V-YDF132 has low requirements on the preservation conditions and can be long-term preserved without losing biological activity.

The invention discloses a gas-producing vibrio strain for producing prodigiosin and a prodigiosin synthetic gene cluster, and belongs to the technical field of microorganisms. The invention relates to a strain B1D10 for producing prodigiosin, the classification name of the strain B1D10 is Vibrio gazogenes, the strain B1D10 is preserved in Guangdong Province Microbial Culture Collection Center, the preservation number is GDMCC NO: 66510, the preservation date is June 13, 2025, and the preservation address is No.59 building, No.100 Courtyard, Xianlie Middle Road, Guangzhou. The strain B1D10 contains a prodigiosin synthetic gene cluster consisting of 16 genes. The strain is rapid in propagation and can be applied to large-scale fermentation production of prodigiosin.

The application discloses a Vibrio parahaemolyticus quorum sensing inhibiting peptide and application thereof, and belongs to the field of bioactive peptides. The inhibiting peptide comprises the following amino acid sequence: Ser-Phe; the application further discloses application of the quorum sensing inhibiting peptide in inhibiting Vibrio parahaemolyticus or preparing antibacterial drugs for inhibiting Vibrio parahaemolyticus. The application adopts a molecular docking technology to screen an active peptide having an inhibiting effect on a Vibrio parahaemolyticus LuxS / AI-2 quorum sensing system, ADMET property prediction is carried out on a peptide segment with better binding force, and finally, a bioactive peptide SF stably combined with LuxP, LuxQ and LuxS is obtained; the dipeptide has good in-vitro inhibiting activity on the Vibrio parahaemolyticus LuxS / AI-2 quorum sensing, and has no cytotoxicity. The dipeptide obtained by the application can be used for guiding development of a new type of antibacterial preparation applied to food, medicine and agriculture and other fields, and has great significance for human health and agricultural development.

This invention provides the application of Vibrio receptor protein type VI collagen α2 from the tongue sole as a target protein for blocking Vibrio pathogen adhesion and infection of tongue sole cells, providing an important foundation for the research and development of strategies and disease control technologies for the prevention and treatment of vibrio infections in tongue sole. This invention discovers that tongue sole type VI collagen α2 can mediate the binding of various Vibrio pathogens to tongue sole, and can serve as a candidate protein for blocking Vibrio adhesion and infection of tongue sole cells, thus providing a new strategy for the prevention and treatment of vibrio diseases in tongue sole. This invention also provides a new approach for the development of novel antibacterial drugs, which has application value in the prevention and treatment of tongue sole diseases.

The present application provides a mouse tail bacteria and application thereof, and belongs to the technical field of biotechnology. Muricauda beolgyonensis SY152020042, the preservation number is CCTCC NO:M 20251495, and the nucleotide sequence is shown as SEQ ID NO:1. The anti-vibrio preparation prepared based on the mouse tail bacteria can achieve broad-spectrum, instant and low-cost vibrio prevention and treatment, and effectively curb the spread of drug resistance and environmental pollution.

The invention discloses a Ruditapes philippinarum miR-263b inhibitor and application thereof, and finds that Ruditapes philippinarum miR-263b can regulate and control innate immune response of a host by targeting RpSC2, negatively regulates activation of an immune-related signal channel in a vibrio infection process, and further, on the basis of a molecular regulation and control mechanism, the Ruditapes philippinarum miR-263b inhibitor can be used for inhibiting the infection of vibrio. The invention discloses a specific inhibitor which is completely complementary with a miR-263b mature body sequence. Experimental data show that after Ruditapes philippinarum is injected with the miR-263b inhibitor, the expression level of miR-263b in vivo is reduced, and the inhibition effect of miR-263b on RpSC2 is effectively relieved, so that the expression level of a target gene RpSC2 is up-regulated, an NF-kappa B immune signal channel is activated, the immune defense capability of the Ruditapes philippinarum on vibrio anguillarum is enhanced, the death rate after infection is remarkably reduced, and the Ruditapes philippinarum is prevented from being infected. The in-vivo bacterium loading amount is obviously reduced, and the pathological injury of hepatopancreas tissue is obviously relieved.

The invention discloses a PtALF-8 gene for coding a portunus trituberculatus anti-lipopolysaccharide factor as well as a protein and application of the PtALF-8 gene, and belongs to the technical field of molecular biology. The nucleotide sequence of the PtALF-8 gene is as shown in SEQ ID No. 1, and the amino acid sequence of the portunus trituberculatus anti-lipopolysaccharide factor protein obtained by encoding the PtALF-8 gene is as shown in SEQ ID No. 2. In-vitro antibacterial experiments prove that the anti-lipopolysaccharide factor protein PtALF-8 has a remarkable inhibition effect on the growth of vibrio parahaemolyticus and staphylococcus caprae. Therefore, the invention lays an application foundation for disease control and gene-assisted breeding of portunus trituberculatus, and has potential market application value in the aspects of development of antibacterial drugs, production of feed additives and the like.

The invention discloses a portunus trituberculatus anti-lipopolysaccharide factor coding gene PtALF-7 as well as a protein and application thereof, and belongs to the technical field of molecular biology. The nucleotide sequence of the coding gene PtALF-7 is as shown in SEQ ID No. 1, and the amino acid sequence of the portunus trituberculatus anti-lipopolysaccharide factor obtained by coding of the coding gene PtALF-7 is as shown in SEQ ID No. 2. In-vitro antibacterial experiments prove that the anti-lipopolysaccharide factor generated by encoding the gene PtALF-7 has a remarkable inhibition effect on the growth of vibrio parahaemolyticus and staphylococcus caprae. Therefore, the invention lays an application foundation for disease control, gene-assisted breeding and the like of the portunus trituberculatus, and has good market application value in the aspects of development of antibacterial drugs, production of feed additives and the like.

The invention discloses a nucleic acid aptamer A4-CA2 with high affinity to vibrio parahaemolyticus and application of the nucleic acid aptamer A4-CA2, and belongs to the technical field of nucleic acid aptamers. The nucleotide sequence of the nucleic acid aptamer A4-CA2 with high affinity to vibrio parahaemolyticus is as shown in SEQ ID NO. 2. The invention further discloses application of the nucleic acid aptamer A4-CA2 with high affinity to the vibrio parahaemolyticus in detection of the vibrio parahaemolyticus and application of the nucleic acid aptamer A4-CA2 in preparation of products for detecting the vibrio parahaemolyticus. The affinity and dissociation constant of the nucleic acid aptamer A4-CA2 and the vibrio parahaemolyticus is 76.5 nM, and the nucleic acid aptamer A4-CA2 has high affinity, does not obviously bind to escherichia coli, staphylococcus aureus, aeromonas salmonicida and bacillus cereus, shows good specificity and is suitable for specific detection of the vibrio parahaemolyticus.