159 results about "Vibrion" patented technology

The invention discloses a vibrio cantoniensis bacteriophage vBVcaSR45Z and an application of the vibrio cantoniensis bacteriophage The invention relates to Vibrio campbelli phage vBVcaSR45Z, which is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on December 8, 2025, the address is Courtyard 100 #, Xianlie Middle Road, Xiaolian District, Guangzhou City, Guangdong Province, the postcode is 510070, and the preservation number is GDMCC NO. 67441-B1. The invention further discloses a preparation method of the Vibrio campbelli phage vBVcaSR45Z strain. The bacteriophage vBVcaSR45Z has the advantages of rapid splitting capacity, high host inhibition effect, safe genome structure and clear systematic classification status, and provides an important basis for the bacteriophage vBVcaSR45Z as a biological prevention and control factor of aquatic vibrio pathogens.

The invention discloses bacillus orientalis ZL3 for regulating rhizosphere soil microecosystems of haloxylon ammodendron soil in deserts and promoting the growth of the haloxylon ammodendron soil, particularly relates to a fungicide ZL3 for promoting the growth of haloxylon ammodendron and regulating rhizosphere microbial communities and application of the fungicide ZL3, and belongs to the technical field of microorganisms. According to the bacillus toonensis ZL3 provided by the invention, the conversion of soil nutrients to available nutrients is improved, and the rhizosphere microbial community structure is improved, so that the effect of promoting the growth of haloxylon ammodendron is achieved. When the ZL3 is applied to a haloxylon ammodendron field, the content of soil organic matters, the content of soil quick-acting potassium, the content of available phosphorus and the activity of sucrase are remarkably improved by 37.78%, 11.01%, 49.66% and 67.71% respectively. The plant height is increased by 17.59%, and the root length is increased by 109.45%. Meanwhile, the positive change of microbial communities is regulated and controlled, especially the enrichment of beneficial bacteria, the relative abundance of the prevotella and the vibrio is remarkably increased, and the relative abundance of the burkholderia is remarkably reduced. In general, inoculation of the ZL3 fungicide can promote the growth and development of haloxylon ammodendron by improving the soil fertility and improving the rhizosphere microflora structure.

The present application belongs to the field of aquaculture technology, and mainly relates to a traditional Chinese medicine composition for preventing and treating acute hepatopancreas necrosis disease of prawn, a preparation method and application. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 28-32 parts of Radix Sanguisorbae, 28-32 parts of Radix Scutellariae, 18-22 parts of Phellodendri Cortex and 8-12 parts of Lonicerae Japonicae Flos. The traditional Chinese medicine composition can significantly inhibit the activity of the main pathogen of the disease, i.e. Vibrio parahaemolyticus, improve the immune function and disease resistance of prawn, and further repair the hepatopancreas and intestinal tissues of the diseased prawn, thereby achieving the effect of effectively preventing and treating the acute hepatopancreas necrosis disease of prawn.

The invention discloses a nano antibody for detecting vibrio parahaemolyticus as well as a preparation method and application thereof, and aims to provide a nano antibody for detecting vibrio parahaemolyticus, which is high in detection accuracy, strong in specificity and simple in obtaining method. The amino acid sequence of the nano antibody is as shown in SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4 or SEQ ID NO. 5. Belongs to the technical field of biological detection.

The invention belongs to the technical field of microorganisms, and discloses a bifidobacterium animalis subsp. Lactis XB02 strain capable of inhibiting pathogenic bacteria and improving inflammation, the preservation number of the bifidobacterium animalis subsp. Lactis XB02 strain is CGMCC (China General Microbiological Culture Collection Center) No.34942, and the preservation date is June 18, 2025. Meanwhile, the probiotic composition adopting the bacterial strain XB02 also comprises a lactobacillus rhamnosus XB01 bacterial strain. The XB02 strain disclosed by the invention has excellent bacteriostatic activity, especially for vibrio parahaemolyticus and gardneria; the atopic dermatitis symptom can be obviously improved; the intestinal mucosa permeability is reduced, and the intestinal barrier function is enhanced; and when the strain is combined with the XB01 strain for use, a synergistic sleep promoting effect is shown. The invention further provides a probiotic composition containing the strain, a preparation and application of the probiotic composition and the preparation in food, medicine or health care products.

The invention discloses a lactobacillus acidophilus FMM01 with the effects of improving the contractility of pelvic floor muscles and repairing mucous membranes and application of the lactobacillus acidophilus FMM01, the lactobacillus acidophilus FMM01 is preserved in the China Center for Type Culture Collection, the preservation date is January 9, 2024, and the preservation number is CCTCC NO: M2024052. The lactobacillus acidophilus FMM01 has the advantages of self-adaption, high acid resistance, strong mucous membrane adhesiveness and no irritation to vaginal mucosa. The lyophilized powder is high in activity and excellent in stability; experimental results show that the lactobacillus acidophilus FMM01 can effectively inhibit bacteria, and especially can effectively inhibit enterobacter cloacae, vibrio parahaemolyticus and / or candida albicans. Besides, the lactobacillus acidophilus FMM01 can improve vaginal relaxation, improve pelvic floor muscle contractility and / or recover vaginal mucosa functions, can promote recovery of healthy florae after mixed vaginitis antibiotic / hormone treatment, improves the treatment effect of mixed vaginitis, and remarkably reduces the recurrence rate of mixed vaginitis.

The application discloses a Vibrio harveyi oral vaccine and a preparation method and application thereof. The application uses a lactococcus lactis NZ9000 expression system, modifies transformation parameters, effectively expresses Vibrio harveyi OmpU protein, constructs a lactococcus lactis (NZ9000) engineering bacterium expressing Vibrio harveyi outer membrane protein (OmpU), develops an oral vaccine against Vibrio harveyi infection, aims to reduce the infection of economic animals in seawater aquaculture by oral administration, provides a new scheme for dealing with the harm of Vibrio harveyi infection and antibiotic resistance and the like, promotes the breeding of fry and fingerlings, and promotes the development of biological agriculture and related industries. The strategy is expected to overcome the problems of traditional injection vaccines, such as inconvenient operation and great stress, and can provide a new technical scheme for reducing the dependence of the aquaculture industry on antibiotics and promoting the establishment of a green and healthy breeding mode.

The invention provides a bacteriostatic agent containing an agilawood leaf extracting solution for inhibiting vibrio alginolyticus and application of the bacteriostatic agent, and relates to the field of plant extracts. The bacteriostatic agent disclosed by the invention is an agilawood leaf extracting solution; the agilawood leaf extracting solution is prepared by adding water into agilawood leaves, crushing and extracting, and the material-liquid ratio of the agilawood leaves to the water is 1g: (2-4mL); the agilawood leaf extracting solution is used for inhibiting vibrio alginolyticus. The preparation method of the agilawood leaf extracting solution comprises the following steps: taking agilawood leaves, adding water, crushing into homogenate, filtering to remove impurities, centrifugally separating, collecting supernate, and sterilizing to obtain the agilawood leaf extracting solution. The minimum inhibitory concentration of the agilawood leaf extracting solution prepared by the invention on vibrio alginolyticus is 15.63 mg / ml. The agilawood leaf extracting solution prepared by the invention can well inhibit vibrio alginolyticus, is used for preparing a bacteriostatic agent for inhibiting vibrio alginolyticus, avoids the risk of drug-resistant genes, has no risk of organic solvent residue, and has the advantages of high efficiency, risk reduction, cost reduction and the like.

The application provides a new bacteriophage for preventing and treating vibrio diseases of aquatic animals and application thereof, the bacteriophage is preserved in China Center for Type Culture Collection on September 4, 2020, and the preservation number is CCTCC M 2020465. The bacteriophage is a new species of bacteriophage of the Caudovirales class and Queuovirinae subfamily, the whole genome of the bacteriophage is OP331229.1 in the NCBI official website GeneBank. The bacteriophage has a wide phage spectrum, can crack 6 different species and genera of vibrio at the same time, can crack 45 strains of Vibrio parahaemolyticus, 22 strains of Vibrio harveyi, 17 strains of Vibrio alginolyticus, 13 strains of Vibrio vulnificus, 11 strains of Vibrio campbellii and 9 strains of Vibrio cholerae at the same time, can more effectively solve the problem of multiple bacterial disease infections in aquaculture, and can be used as a microecological preparation for preventing and treating vibrio diseases such as Vibrio parahaemolyticus, Vibrio harveyi and Vibrio alginolyticus of aquatic animals.

The application discloses a freeze-drying protective agent of Vibrio harveyi phage V-YDF132 and a preservation method thereof. The freeze-drying protective agent contains inulin, skimmed milk powder, gelatin, L-glutamic acid sodium and water. The freeze-drying protective agent is mixed with a phage V-YDF132 solution, and then the mixture is frozen to a solid state, and after being subjected to freeze-drying, a phage V-YDF132 freeze-drying powder preparation is obtained. There are various preservation methods for phages, and the best preservation method is different for different phages. The application screens and prepares different phage freeze-drying protective agents and explores the protection effect of the freeze-drying protective agents on phages after freeze-drying, so as to lay a foundation for phage preservation. The phage powder preparation prepared from the freeze-drying protective agent formula P9 and the phage V-YDF132 has low requirements on the preservation conditions and can be long-term preserved without losing biological activity.

The application discloses a Vibrio parahaemolyticus quorum sensing inhibiting peptide and application thereof, and belongs to the field of bioactive peptides. The inhibiting peptide comprises the following amino acid sequence: Ser-Phe; the application further discloses application of the quorum sensing inhibiting peptide in inhibiting Vibrio parahaemolyticus or preparing antibacterial drugs for inhibiting Vibrio parahaemolyticus. The application adopts a molecular docking technology to screen an active peptide having an inhibiting effect on a Vibrio parahaemolyticus LuxS / AI-2 quorum sensing system, ADMET property prediction is carried out on a peptide segment with better binding force, and finally, a bioactive peptide SF stably combined with LuxP, LuxQ and LuxS is obtained; the dipeptide has good in-vitro inhibiting activity on the Vibrio parahaemolyticus LuxS / AI-2 quorum sensing, and has no cytotoxicity. The dipeptide obtained by the application can be used for guiding development of a new type of antibacterial preparation applied to food, medicine and agriculture and other fields, and has great significance for human health and agricultural development.

This invention provides the application of Vibrio receptor protein type VI collagen α2 from the tongue sole as a target protein for blocking Vibrio pathogen adhesion and infection of tongue sole cells, providing an important foundation for the research and development of strategies and disease control technologies for the prevention and treatment of vibrio infections in tongue sole. This invention discovers that tongue sole type VI collagen α2 can mediate the binding of various Vibrio pathogens to tongue sole, and can serve as a candidate protein for blocking Vibrio adhesion and infection of tongue sole cells, thus providing a new strategy for the prevention and treatment of vibrio diseases in tongue sole. This invention also provides a new approach for the development of novel antibacterial drugs, which has application value in the prevention and treatment of tongue sole diseases.

The present application provides a mouse tail bacteria and application thereof, and belongs to the technical field of biotechnology. Muricauda beolgyonensis SY152020042, the preservation number is CCTCC NO:M 20251495, and the nucleotide sequence is shown as SEQ ID NO:1. The anti-vibrio preparation prepared based on the mouse tail bacteria can achieve broad-spectrum, instant and low-cost vibrio prevention and treatment, and effectively curb the spread of drug resistance and environmental pollution.

The invention discloses a Ruditapes philippinarum miR-263b inhibitor and application thereof, and finds that Ruditapes philippinarum miR-263b can regulate and control innate immune response of a host by targeting RpSC2, negatively regulates activation of an immune-related signal channel in a vibrio infection process, and further, on the basis of a molecular regulation and control mechanism, the Ruditapes philippinarum miR-263b inhibitor can be used for inhibiting the infection of vibrio. The invention discloses a specific inhibitor which is completely complementary with a miR-263b mature body sequence. Experimental data show that after Ruditapes philippinarum is injected with the miR-263b inhibitor, the expression level of miR-263b in vivo is reduced, and the inhibition effect of miR-263b on RpSC2 is effectively relieved, so that the expression level of a target gene RpSC2 is up-regulated, an NF-kappa B immune signal channel is activated, the immune defense capability of the Ruditapes philippinarum on vibrio anguillarum is enhanced, the death rate after infection is remarkably reduced, and the Ruditapes philippinarum is prevented from being infected. The in-vivo bacterium loading amount is obviously reduced, and the pathological injury of hepatopancreas tissue is obviously relieved.

The invention discloses a PtALF-8 gene for coding a portunus trituberculatus anti-lipopolysaccharide factor as well as a protein and application of the PtALF-8 gene, and belongs to the technical field of molecular biology. The nucleotide sequence of the PtALF-8 gene is as shown in SEQ ID No. 1, and the amino acid sequence of the portunus trituberculatus anti-lipopolysaccharide factor protein obtained by encoding the PtALF-8 gene is as shown in SEQ ID No. 2. In-vitro antibacterial experiments prove that the anti-lipopolysaccharide factor protein PtALF-8 has a remarkable inhibition effect on the growth of vibrio parahaemolyticus and staphylococcus caprae. Therefore, the invention lays an application foundation for disease control and gene-assisted breeding of portunus trituberculatus, and has potential market application value in the aspects of development of antibacterial drugs, production of feed additives and the like.

The invention discloses a portunus trituberculatus anti-lipopolysaccharide factor coding gene PtALF-7 as well as a protein and application thereof, and belongs to the technical field of molecular biology. The nucleotide sequence of the coding gene PtALF-7 is as shown in SEQ ID No. 1, and the amino acid sequence of the portunus trituberculatus anti-lipopolysaccharide factor obtained by coding of the coding gene PtALF-7 is as shown in SEQ ID No. 2. In-vitro antibacterial experiments prove that the anti-lipopolysaccharide factor generated by encoding the gene PtALF-7 has a remarkable inhibition effect on the growth of vibrio parahaemolyticus and staphylococcus caprae. Therefore, the invention lays an application foundation for disease control, gene-assisted breeding and the like of the portunus trituberculatus, and has good market application value in the aspects of development of antibacterial drugs, production of feed additives and the like.

The invention discloses a nucleic acid aptamer A4-CA2 with high affinity to vibrio parahaemolyticus and application of the nucleic acid aptamer A4-CA2, and belongs to the technical field of nucleic acid aptamers. The nucleotide sequence of the nucleic acid aptamer A4-CA2 with high affinity to vibrio parahaemolyticus is as shown in SEQ ID NO. 2. The invention further discloses application of the nucleic acid aptamer A4-CA2 with high affinity to the vibrio parahaemolyticus in detection of the vibrio parahaemolyticus and application of the nucleic acid aptamer A4-CA2 in preparation of products for detecting the vibrio parahaemolyticus. The affinity and dissociation constant of the nucleic acid aptamer A4-CA2 and the vibrio parahaemolyticus is 76.5 nM, and the nucleic acid aptamer A4-CA2 has high affinity, does not obviously bind to escherichia coli, staphylococcus aureus, aeromonas salmonicida and bacillus cereus, shows good specificity and is suitable for specific detection of the vibrio parahaemolyticus.

The application discloses a Vibrio parahaemolyticus CJ5 and application thereof, and belongs to the technical field of microorganisms. The Vibrio parahaemolyticus CJ5 provided by the application is separated and screened from workshop water of a sea cucumber breeding base, the strain can efficiently and directionally degrade SC-FUC, the degradation product has a relatively high sulfate retention rate and a relatively concentrated molecular mass distribution, under the conditions that the growth temperature is 30 DEG C, the salinity is 3%, the pH value is 8.0 and the inoculation amount is 3%, the degradation rate of the strain to SC-FUC reaches 61.47%, the sulfate mass fraction of the degradation product is 34.55%, and the relative molecular mass distribution range is within 800 Da, so that the sea cucumber fucoidan with DP 2-5 can be prepared.

The application discloses specific molecular targets of Vibrio alginolyticus and Vibrio fluvialis and a detection method thereof, wherein the nucleotide sequence of the specific molecular target of the Vibrio alginolyticus is as shown in SEQ ID NO. 1, and the nucleotide sequence of the specific molecular target of the Vibrio fluvialis is as shown in SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4 or SEQ ID NO. 5. The application not only discloses one specific molecular target for identifying the Vibrio alginolyticus and four specific molecular targets for identifying the Vibrio fluvialis, but also provides related specific primer sequences and corresponding PCR detection methods. The application has the advantages of short detection time, low cost, strong specificity and the like without physiological and biochemical identification.

The invention discloses an application of nomuraea sp. 18B9 in resisting pathogenic vibrio. The nomuraea sp. 18B9 can be used for resisting pathogenic vibrio. The vibrio is not limited to vibrio candersonii, vibrio mimicus and vibrio parahaemolyticus.

The application discloses a portunus trituberculatus anti-lipopolysaccharide factor coding gene PtALF-7 and a protein and application thereof, and belongs to the technical field of molecular biology. PtALF-7 The nucleotide sequence of the coding gene is shown as SEQ ID No. 1, and the amino acid sequence of the obtained portunus trituberculatus anti-lipopolysaccharide factor is shown as SEQ ID No. 2. PtALF-7 The in-vitro bacteriostatic experiment proves that the anti-lipopolysaccharide factor coded by the gene has a significant inhibiting effect on the growth of Vibrio parahaemolyticus and Staphylococcus caprae. Therefore, the application lays an application foundation for disease prevention of portunus trituberculatus, gene-assisted breeding and the like, and has good market application value in the development of antibacterial drugs, feed additive production and the like.

This invention discloses the application of L-lysine in improving the sensitivity of Vibrio alginolyticus to clindamycin. This invention discovers that the combined use of L-lysine and clindamycin can significantly increase reactive oxygen species within Vibrio alginolyticus, thereby increasing the amount of clindamycin entering the bacteria and ultimately leading to bacterial death. Therefore, L-lysine can improve bacterial sensitivity to clindamycin, thus overcoming the problem of bacterial resistance. The small molecule metabolite provided by this invention, when used in combination with clindamycin, can significantly enhance the bactericidal effect of clindamycin, exhibiting better efficacy, higher safety, and greater operability compared to existing methods that use clindamycin alone as an antibacterial drug.

The invention discloses separation and application of a vibrio parahaemolyticus lytic bacteriophage vBVpaSR27Z (Vibrio parahaemolyticus lytic bacteriophage). The preservation number of the bacteriophage vBVpaSR27Z is GDMCC No: 67438-B1 (GDMCC No: 1). The bacteriophage is separated from an environmental sample by adopting a double-layer plate method, the head of the bacteriophage is in a regular icosahedron shape (84 nm * 71 nm), the tail length of the bacteriophage is about 141 nm, the incubation period of the bacteriophage is only 20 min, the lysis speed is high, and the activity of the bacteriophage is stable within the pH range of 5-10 and the temperature range of 4-37 DEG C; the genome of the vibrio bacteriophage is double-stranded DNA without known drug resistance and toxicity genes, phylogenetic analysis shows that the vibrio bacteriophage has low homology with known vibrio bacteriophages, and the vibrio bacteriophage belongs to a novel genetic resource. The vBVpaSR27Z can rapidly inhibit the growth of strains such as vibrio parahaemolyticus, is suitable for green prevention and control of diseases such as vibrio parahaemolyticus in aquaculture, and can be used as an active component of a biological antibacterial preparation.

The invention discloses five food-borne pathogenic bacteria LAMP method high-throughput detection primer groups, a kit and application, and belongs to the technical field of food-borne pathogenic bacteria detection. The primer group corresponds to five bacteria, namely salmonella, vibrio parahaemolyticus, listeria monocytogenes, escherichia coli O157: H7 and staphylococcus aureus, and the nucleotide sequences of the primers are SEQ ID No.1-30. A matched system can complete the whole-process detection at the constant temperature of 62 DEG C for 45 minutes, the result does not need a complicated instrument, the positive grey and negative purple are judged by naked eyes, and the operation is simple and convenient; the detection sensitivity reaches 1-75 copies / reaction, an artificially polluted aquatic product sample does not need to be subjected to enrichment and is subjected to simple splitting decomposition and enrichment, the specificity is high, and no cross reaction exists; the anti-interference capability is outstanding, the method adapts to complex matrixes of aquatic products, and the detection coincidence rate is high; the device is simple in structure and low in cost, only needs a conventional constant temperature device, is suitable for basic food, agriculture, disease control, customs and other detection institutions, food enterprises, on-site law enforcement and on-site detection and other scenes, and provides an efficient and economic technical means for aquatic product safety management and control.

The invention discloses a portable electrochemical biosensor for detecting vibrio parahaemolyticus, the principle of the invention can be also suitable for detection of other pathogenic microorganisms, and the portable electrochemical biosensor comprises a PVC substrate, a sample pad, a capture pad, a screen printing electrode and a water absorption pad. According to the invention, the advantage of high sensitivity of electrochemistry and the advantage of no separation of the test strip are combined together, the vibrio parahaemolyticus is captured by using the aptamer and is fixed on the detection line, the signal probe is dropwise added, the electrochemical workstation is used for detection, and quantitative detection of the vibrio parahaemolyticus is realized by adopting a signal subtraction output mode. The method has the advantages of low cost, high detection speed, high sensitivity, simplicity in operation and the like. The requirements of customers for on-site rapid detection can be met, and food poisoning of patients can be effectively prevented.

The present application relates to the field of microorganisms, and in particular to a strain of Vibrio gigas for removing calcium ions in high-salt hard water and its application. The strain is named Vibrio gigas DZWSW, and was preserved in the China General Microbiological Culture Collection Center on December 20, 2021, with the preservation number of CGMCC No. 24153. The Vibrio gigas DZWSW provided in the present application plays an important role in precipitating calcium ions in high-salt hard water, and has a broad application prospect in hard water softening. After the Vibrio gigas DZWSW provided in the present application precipitates calcium ions in high-salt hard water, carbonate mineral precipitates are formed, including calcite, single-water calcite and dolomite minerals; the formed mineral precipitates can be used in the field of building materials, and have a broad application prospect.

This invention discloses a method for cultivating pearl grouper, belonging to the field of aquaculture technology. Through careful design of active component configuration and juvenile feeding according to size, component A includes the following raw materials: β-chitosan, oxalic acid, and *Gnaphalium affine* extract; component B includes the following raw materials: sugarcane extract, rye seed powder, and oryzanol; component C includes the following raw materials: fig polysaccharide, yeast β-glucan, and eucalyptus oil. When the juvenile size is 2.2~5.0cm, a basic feed supplemented with components A and B is provided; when the juvenile size is 5.1~10cm, a basic feed supplemented with components A and C is provided; when the juvenile size is greater than 10cm, a basic feed supplemented with components A, B, and C is provided. The cultivation method of this application can improve the resistance of pearl grouper to *Vibrio harveyi* infection and / or its low-temperature tolerance, and the cultivation method is simple and the raw materials are readily available.

The application discloses a method for leaching waste lithium battery positive pole powder in a neutral environment by using common desulfurization vibrio, and belongs to the field of waste battery metal resource recycling and bio-metallurgical technology. The common desulfurization vibrio obtained through gradient domestication is inoculated into a modified culture medium, and then the bacterial liquid is activated to construct a bioleaching system, so that the valuable metals in waste NCM, NCA, LFP, LCO, LMO and other positive pole materials are bioleached. The common desulfurization vibrio is innovatively used as the leaching strain, and the dependence on acidophilic bacteria in traditional bioleaching is abandoned, so that the efficient dissolution and directional separation of valuable metals in various waste LIBs positive pole materials are realized under mild neutral conditions, and a new microbial technical scheme is provided for waste LIBs positive pole valuable metal recovery.

The invention provides a phage E protein mutant and application thereof. Compared with the phage E protein, the phage E protein mutant has the advantage that the 33rd lysine is mutated into arginine. According to the invention, the cracking capability of the K33R variant under the background of the vibrio natriticus MraY is screened and proved to be obviously superior to that of a wild type E protein, so that the blank of lack of a standard cracking element for the vibrio natriticus in the prior art is filled; and an effect module which can be directly applied is provided for realizing stable oscillation of an ePop self-splitting line in the vibrio natriticus or engineering bacteria carrying the vibrio natriticus MraY.