561 results about "Vibrion" patented technology

The invention belongs to the technical field of aquatic biotechnology and molecular marker-assisted breeding, and particularly relates to a cynoglossus semilaevis vibrio harveyi-resistant KASP molecular marker combination and application thereof in cynoglossus semilaevis disease-resistant breeding. The KASP molecular marker combination comprises three SNP (Single Nucleotide Polymorphism) loci which are respectively positioned at 17314099, 17325496 and 17355989 loci of the 14 # chromosome of the cynoglossus semilaevis, when the genotypes of the three SNP loci are respectively CC, AA and GG, the disease resistance of the cynoglossus semilaevis is high, and the invention also provides a primer for detecting the genotype polymorphism of the SNP loci. By utilizing the KASP molecular marker combination, the vibrio harveyi resistance genotype in a cynoglossus semilaevis breeding population can be quickly detected, and disease-resistant individuals are screened, so that the disease-resistant breeding efficiency of the cynoglossus semilaevis is greatly improved, and a new variety of the cynoglossus semilaevis with strong disease resistance can be quickly cultivated.

The invention discloses an antibacterial peptide and application thereof, and belongs to the technical field of biology. The antibacterial peptide with an inhibition effect on various clinically common pathogenic microorganisms is obtained, and the antibacterial peptide comprises gram-negative bacteria such as escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, acinetobacter baumannii, vibrio parahaemolyticus, salmonella gallinarum, salmonella typhimurium and enterobacter sakazakii, and gram-positive bacteria. The antibacterial peptide provided by the invention can be used as an antibacterial peptide, such as staphylococcus aureus, listeria monocytogenes and bacillus cereus, and the antibacterial peptide provided by the invention is novel in structure and easy to prepare.

The invention provides a virulent bacteriophage and application thereof, the virulent bacteriophage is named as vBVpaPChan1 and has been preserved in China Center for Type Culture Collection on December 10, 2024, the preservation address is Wuhan University, Wuhan, Hubei, China, and the preservation number is CCTCC M 20242772. The bacteriophage is safe, environmentally friendly and free of toxic and side effects, and compared with known bacteriophages, the bacteriophage is short in incubation period, large in outbreak amount and capable of effectively killing diseases caused by vibrio parahaemolyticus chan02.

The invention discloses a lytic bacteriophage vBVcaPR25D and an application of the lytic bacteriophage vBVcaPR25D. The collection number of the lytic bacteriophage vBVcaPR25D is GDMCC (Graphical Digital Microbial Culture Collection Center) No: 65167-B1. The lytic bacteriophage vBVcaPR25D provided by the invention has a high specificity and a remarkable killing effect on vibrio cantoniensis V.campbelli LMG 11216T, and can be used for killing vibrio cantoniensis V.campbelli LMG 11216T. Besides, virulence factor genes and drug-resistant genes are not detected in the genome of the bacteriophage, so that the bacteriophage is high in safety and environment-friendly, and can be used as a bactericide for aquatic vibrio candersonii diseases.

The invention discloses a virulent bacteriophage vBValSR36H with a host being vibrio alginolyticus and application of the virulent bacteriophage vBValSR36H, and relates to the technical field of microbial control. The invention provides a bacteriophage capable of efficiently cracking Vibrio alginolyticus ATCC 17749T, the bacteriophage is named as vBValSR36H, the preservation number is 66248-B1, and the preservation address is Institute of Microbiology, Academy of Sciences in Guangdong Province. The vibrio alginolyticus bacteriophage vBValSR36H provided by the invention has good environmental stability and certain bactericidal activity, and has a relatively wide environmental adaptation range and relatively long survival time. The bacteriophage vBValSR36H provided by the invention can specifically and effectively kill, prevent and control related vibrio diseases, meanwhile, virulence factor genes and drug-resistant genes are not detected in the bacteriophage, and the bacteriophage vBValSR36H has the advantages of no toxic or side effect, high safety, environmental friendliness and wide application prospect. Therefore, the vibrio alginolyticus bacteriophage vBValSR36H can be completely applied to killing and / or preventing vibrio alginolyticus for non-disease diagnosis and treatment purposes.

The invention discloses a broad-spectrum lytic bacteriophage for preventing and controlling various vibrio infections in an aquaculture environment. The invention relates to V.alginolyticus phage vBValM-R37J, which is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on August 6, 2025, the address is No.100 Courtyard, Xianlie Middle Road, Xianlie District, Guangzhou City, Guangdong Province, the postcode is 510070, and the preservation number is GDMCC NO.66813-B1. The invention further discloses a method for preparing the V.alginolyticus phage vBValM-R37J strain. The bacteriophage R37J is a candidate therapeutic bacteriophage with wide host spectrum, high splitting efficiency, gene safety and environmental stability, and has the potential of being popularized and applied in aquaculture as a novel biological tool for accurately preventing and controlling various vibrio infections. A new biological resource is provided for accurate prevention and control of aquatic pathogenic bacteria, and a theoretical basis and a technical support are provided for popularization and application of a phage therapy in an actual culture system.

The invention discloses a lytic bacteriophage vBVcaPR24D and an application of the lytic bacteriophage vBVcaPR24D. The collection number of the lytic bacteriophage vBVcaPR24D is GDMCC (Graphical Digital Microbial Culture Collection Center) No: 65166-B1. The lytic bacteriophage vBVcaPR24D provided by the invention has a high specificity and a remarkable killing effect on vibrio cantoniensis V.campbelli LMG 11216T, and can be used for killing vibrio cantoniensis V.campbelli LMG 11216T. Besides, virulence factor genes and drug-resistant genes are not detected in the genome of the bacteriophage, so that the bacteriophage is high in safety and environment-friendly, and can be used as a bactericide for aquatic vibrio candersonii diseases.

The invention belongs to the technical field of molecular biology, and particularly relates to a nucleic acid sequence combination for simultaneously detecting various pathogenic vibrios, an RT-PCR (reverse transcription-polymerase chain reaction) kit and a use method of the RT-PCR kit, and the nucleic acid sequence combination is shown as SEQ ID No.1-SEQ ID No.19. The RT-PCR kit comprises RT-PCR primer probes, the primer probes comprise forward and reverse amplification primers and detection probes respectively aiming at vibrio cholerae, vibrio parahaemolyticus, vibrio fluvialis, vibrio mimicus, vibrio vulnificus and vibrio alginolyticus, and corresponding sequences in SEQ ID No.1-SEQ ID No.19 are adopted. The kit disclosed by the invention can be used for rapidly detecting and identifying the six vibrios at the same time; the kit has high accuracy, specificity and sensitivity, and is suitable for clinical detection of infected samples.

The invention discloses a compound microorganism and application thereof to improvement of saline-alkali soil, and relates to the technical field of agriculture, the compound microorganism is obtained by compounding halophilic bacillus, rhodotorula mucilaginosa, alkali-resistant rhizobium, desulfurization vibrio and siliconizing bacteria, and the excellent saline-alkali soil improvement effect can be achieved. The functional strains cooperate with one another, have complementary advantages, and play a role in multiple aspects such as soil structure improvement, nutrient supply and absorption, salt and alkali relief, microbial community optimization and the like. The compound microbial preparation has an efficient synergistic mechanism, provides an innovative solution for solving the problem of saline-alkali soil, and shows huge potential in the aspects of promoting agricultural green development and ecological protection.

The invention belongs to the technical field of biology, and provides enterococcus faecalis, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No.29087. The enterococcus faecalis is characterized in that the enterococcus faecalis can secrete bacteriocin for inhibiting vibrio parahaemolyticus, staphylococcus aureus and escherichia coli. The invention also provides the enterococcus faecalis and application thereof in an aquatic product fermented feed and a laying hen premix, and the enterococcus faecalis can effectively improve the utilization rate of the feed and the growth and production performance of animals.

The invention firstly provides application of nonanoic acid in artemia culture. In the application, the nonanoic acid takes silicon dioxide or chitosan as a carrier and is directly added into a culture water body. Wherein when the nonanoic acid takes silicon dioxide as a carrier, the use concentration of the nonanoic acid in a water body is 150-300 micrometers; when chitosan is used as a carrier of the nonanoic acid, the use concentration of the nonanoic acid in the water body is 55-90 micrometers. The inventor accidentally finds that after the nonanoic acid is mixed with the silicon dioxide or the chitosan and then added into the culture water body, the sudden drop of the pH value of the water body cannot be caused, so that an acid-base regulator does not need to be added, and the incubation and growth of artemia cannot be influenced; meanwhile, uniform dispersion and local high concentration of the nonanoic acid in the water body are realized by using the carrier, so that the use amount of the acid is reduced, and the antibacterial effect is remarkably improved. The invention further provides an artemia breeding method adopting the nonanoic acid as the antibody replacement product. According to the artemia obtained through the breeding method, the hatching rate and the survival rate of the artemia are remarkably increased, the number of vibrios carried by the artemia is effectively reduced, expression of related genes such as growth and immunity of the artemia is up-regulated, development and growth of the artemia are promoted, ecological safety is improved, and unexpected technical effects are achieved.

Provided are a method for controlling a soilborne disease in which a load on an environment and a human body is reduced, and a novel predatory bacterium therefor.　Provided are: a predatory bacterium or a variant thereof selected from the group consisting of bacteria of the genus Bacteriovorax and bacteria of the genus Bdellovibrio which selectively prey on a pathogenic bacterium belonging to the family Burkholderiaceae, the family Pectobacteriaceae, or the family Lysobacteraceae; a method for controlling a soilborne plant disease caused by the pathogenic bacterium, the method characterized in that the predatory bacterium or variant thereof is applied to a seed, root, or underground stem of a plant, rhizosphere soil, or soil; and a composition.

The invention discloses an aquaculture denitrification microcapsule as well as a preparation method and application thereof, and relates to the technical field of aquaculture and biology. The lactobacillus casei provided by the invention can secrete acid metabolites, and the acid metabolites can inhibit pathogenic vibrio, so that the occurrence of diseases in aquaculture can be reduced. Lactobacillus casei has the efficacy of efficiently degrading ammonia nitrogen and nitrite nitrogen in the aquaculture environment, and can continuously realize efficient cleaning of the aquaculture environment. In addition, the invention also provides a denitrification microcapsule which creates a more favorable microenvironment for the efficient denitrification strain, so that the repeated denitrification performance is more stably exerted, and the denitrification microcapsule can be repeatedly utilized.

The invention belongs to the technical field of gene engineering, and particularly relates to ALFPm3 modified antibacterial peptide, a molecular design and construction method and application of the ALFPm3 modified antibacterial peptide, and the amino acid sequence of the ALFPm3 modified antibacterial peptide is selected from any one of SEQ ID NO: 1-SEQ ID NO: 7. Analysis and design are carried out by combining the structure and action site of vibrio parahaemolyticus and the molecular structure and function relationship of the antibacterial peptide ALFPm3 as a basic template, the antibacterial activity of the antibacterial peptide ALFPm3 is improved, and other biological activities such as hemolysis are reduced.

The invention discloses a concrete crack self-repairing material based on microorganism immobilization and a preparation method of the concrete crack self-repairing material, and belongs to the crossing field of microorganism materials and civil engineering. The material is prepared from peptone, sodium lactate, volcanic rock, bacillus pasteurii and other raw materials through the steps of culture medium preparation, carrier modification, microorganism immobilization, microcapsule and gel protection layer formation and the like, the prepared volcanic rock-chitosan carrier provides an inhabiting environment for microorganisms, and the survival rate of the microorganisms in a high-alkali environment is increased to 70%. When the concrete cracks and the alkaline environment triggers the protective layer to disintegrate, the bacillus pasteurii and the desulfurization vibrio are subjected to a reduction reaction with sulfate through urealysis, and calcium carbonate and calcium sulfide are generated to fill cracks. The 28-day repair rate of the material is larger than or equal to 99%, the maximum crack repair width reaches 1.2 mm, the compressive strength recovery rate is larger than or equal to 85%, excellent durability and environment friendliness are achieved, and the concrete crack repair problem is effectively solved.

The invention provides a lactobacillus pentosus and a preparation method and application of bacteriocin of the lactobacillus pentosus. The name of the strain is lactobacillus pentosus YCL31, and the strain is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on June 25, 2025, the preservation address is the 5th floor of the building 59, No. 100 courtyard, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCC No: 66589. The bacteriocin separated and purified from the metabolite of the bacterium can destroy the morphological structure of the vibrio parahemolyticus and inhibit the toxicity of the vibrio parahemolyticus, can also achieve the effect of remarkably inactivating the vibrio parahemolyticus in a metapenaeus ensis system, can effectively delay the spoilage of the metapenaeus ensis, and has a good application prospect as a bacteriostatic agent or preservative. Therefore, development and application of the novel bacteriocin have important reference value in related fields.

The invention discloses a marine streptomyces strain SHEa3, a compound derived from the marine streptomyces strain SHEa3 and application of the marine streptomyces strain SHEa3, the classification name of the marine streptomyces strain SHEa3 is Streptomyces sp, the preservation number of the marine streptomyces strain SHEa3 is GDMCC No: 66667, and the preservation date of the marine streptomyces strain SHEa3 is July 14, 2025. The invention also discloses a sesquiterpenoids compound streptaviol A derived from marine streptomyces and application of the sesquiterpenoids compound streptaviol A in preparation of drugs for preventing and treating vibrio diseases of mariculture animals. The invention further discloses application of the sesquiterpenoids, namely the sea threitol C, derived from the marine streptomyces in preparation of drugs for preventing and treating vibrio diseases of mariculture animals. The marine streptomyces source compound has efficient anti-vibrio activity, and the marine streptomyces source compound streptaviol A and the helithreitol C are used for preparing the medicine for preventing and treating vibrio diseases of mariculture animals, so that the side effect is low, and the activity is excellent.

The invention belongs to the technical field of microorganisms, and discloses a high-temperature-resistant vibrio alginolyticus bacteriophage and a composition and application thereof, the high-temperature-resistant vibrio alginolyticus bacteriophage is named as vibrio alginolyticus bacteriophage PJ644 and is preserved in China General Microbiological Culture Collection Center on August 16, 2024, the preservation address is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.9624. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No. The bacteriophage has excellent high temperature resistance, and the bacteriophage and the bacteriophage composition not only can effectively prevent and treat various vibrio infections, but also can be used as active ingredients for preparing bacteriophage pharmaceutical preparations, aquatic feed additives, water disinfectants and aquatic product preservatives, and are used for effectively preventing and treating diseases caused by vibrio infections in aquaculture farms. The use is safe, and the problems of antibiotic residues and pathogen drug resistance caused by use of antibiotics are effectively avoided.

The invention belongs to the field of gene engineering, and particularly relates to an ecdysone receptor gene LvECR and application thereof. The base sequence of the ecdysone receptor gene LvECR is as shown in SEQ ID NO: 1. The amino acid sequence of the protein encoded by the LvECR gene is as shown in SEQ ID NO: 2. The invention also discloses an application of the ecdysone receptor gene LvECR in inhibition of pathogenic vibrio infection. It is verified for the first time that the prawn ecdysone receptor gene LvECR plays an important role in prawn acute hepatopancreatic necrosis, disease occurrence can be aggravated by inhibiting expression of the coding gene of the prawn ecdysone receptor gene LvECR, disease symptoms can be reduced by injecting in-vitro recombinant protein of the factor, and the huge application potential of the prawn ecdysone receptor gene LvECR in disease prevention and control is shown.

The invention discloses a vibrio cantoniensis bacteriophage vBVcaSR45Z and an application of the vibrio cantoniensis bacteriophage The invention relates to Vibrio campbelli phage vBVcaSR45Z, which is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on December 8, 2025, the address is Courtyard 100 #, Xianlie Middle Road, Xiaolian District, Guangzhou City, Guangdong Province, the postcode is 510070, and the preservation number is GDMCC NO. 67441-B1. The invention further discloses a preparation method of the Vibrio campbelli phage vBVcaSR45Z strain. The bacteriophage vBVcaSR45Z has the advantages of rapid splitting capacity, high host inhibition effect, safe genome structure and clear systematic classification status, and provides an important basis for the bacteriophage vBVcaSR45Z as a biological prevention and control factor of aquatic vibrio pathogens.

The invention relates to the technical field of medicines, in particular to an antibacterial composition as well as a preparation method and application thereof. The invention provides an antibacterial composition. The antibacterial composition is prepared from the following components in parts by mass: 0.039 to 0.3125 part of SU3327 and 62.5 to 500 parts of citric acid. The combined use of the SU3327 and the citric acid can effectively kill and / or inhibit vibrio parahaemolyticus, escherichia coli, salmonella, acinetobacter baumannii, staphylococcus aureus, klebsiella pneumoniae and staphylococcus pseudointermediates, and the use amount of the SU3327 in the medicament for treating bacterial infection is greatly reduced; and the compound shows extremely high application value in the process of preparing medicines for treating bacterial infection.

The invention belongs to the technical field of aquaculture biological breeding, and particularly relates to a KASP marker of a vibrio harveyi resistant gene jam2a of cynoglossus semilaevis and application of the KASP marker. The KASP markers are respectively positioned at 14: 17 279470, 14: 17 281640 and 14: 17 283306 sites of the 14 # chromosome of the cynoglossus semilaevis, the polymorphism of the sites is T / C, G / T and T / A, and when the genotypes of the three sites are respectively homozygous TT, homozygous GG and homozygous TT, the tested individual of the cynoglossus semilaevis is a disease-resistant individual. The KASP marker significantly associated with the jam2a gene provided by the invention can be used for molecular marker-assisted breeding of the vibriosis resistance character of the cynoglossus semilaevis, and provides important technical support for improving the breeding efficiency of disease resistance of the cynoglossus semilaevis.

The invention discloses a recombinant vibrio natrio as well as a construction method and application thereof, and belongs to the technical field of bioengineering.The recombinant vibrio natrio is an engineering strain for overexpressing vibrio natrio MtrC, MtrA, MtrB, PdsA and CymA proteines.The vibrio natrio is selected as a chassis microorganism for synthesizing selenium nanoparticles, expression of heterologous genes is enhanced by introducing plasmid vectors, and the recombinant vibrio natrio is obtained. The Mtr pathway expression of the sodium selenite reduced by the vibrio natrio is enhanced, and the synthesis of biological nano-selenium by metabolism of organic waste glycerol by the vibrio natrio is realized, so that the production cycle of the nano-selenium can be remarkably shortened, and the production cost can be greatly reduced by using the organic waste as a carbon source to carry out a production experiment. The economic and environmental benefits in the synthesis process of the biological nano-selenium are realized.

The invention relates to the technical field of gene engineering, in particular to two cynoglossus semilaevis vibriosis-resistant genes and an application method thereof. According to the invention, the effects of the CD79a and the CD79b in the cynoglossus semilaevis in resisting vibriosis are mainly studied, verification is respectively carried out in vivo and in vitro, the process of disease-resistant breeding of the cynoglossus semilaevis is further promoted, and it is indicated that the CD79a and the CD79b have the effect of inhibiting vibrio harveyi.

The invention discloses bacillus orientalis ZL3 for regulating rhizosphere soil microecosystems of haloxylon ammodendron soil in deserts and promoting the growth of the haloxylon ammodendron soil, particularly relates to a fungicide ZL3 for promoting the growth of haloxylon ammodendron and regulating rhizosphere microbial communities and application of the fungicide ZL3, and belongs to the technical field of microorganisms. According to the bacillus toonensis ZL3 provided by the invention, the conversion of soil nutrients to available nutrients is improved, and the rhizosphere microbial community structure is improved, so that the effect of promoting the growth of haloxylon ammodendron is achieved. When the ZL3 is applied to a haloxylon ammodendron field, the content of soil organic matters, the content of soil quick-acting potassium, the content of available phosphorus and the activity of sucrase are remarkably improved by 37.78%, 11.01%, 49.66% and 67.71% respectively. The plant height is increased by 17.59%, and the root length is increased by 109.45%. Meanwhile, the positive change of microbial communities is regulated and controlled, especially the enrichment of beneficial bacteria, the relative abundance of the prevotella and the vibrio is remarkably increased, and the relative abundance of the burkholderia is remarkably reduced. In general, inoculation of the ZL3 fungicide can promote the growth and development of haloxylon ammodendron by improving the soil fertility and improving the rhizosphere microflora structure.

The present application belongs to the field of aquaculture technology, and mainly relates to a traditional Chinese medicine composition for preventing and treating acute hepatopancreas necrosis disease of prawn, a preparation method and application. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 28-32 parts of Radix Sanguisorbae, 28-32 parts of Radix Scutellariae, 18-22 parts of Phellodendri Cortex and 8-12 parts of Lonicerae Japonicae Flos. The traditional Chinese medicine composition can significantly inhibit the activity of the main pathogen of the disease, i.e. Vibrio parahaemolyticus, improve the immune function and disease resistance of prawn, and further repair the hepatopancreas and intestinal tissues of the diseased prawn, thereby achieving the effect of effectively preventing and treating the acute hepatopancreas necrosis disease of prawn.

The invention belongs to the technical field of microbial antibacterial preparations, and particularly discloses a novel strong vibrio parahaemolyticus bacteriophage isolated strain vBVpSCC6 and application of the novel strong vibrio parahaemolyticus bacteriophage isolated strain vBVpSCC6. The bacteriophage vBVpSCC6 is preserved in the Guangdong Microbial Culture Collection Center on September 14, 2020, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 61189-B1. The bacteriophage has good tolerance to environments such as temperature, pH, ultraviolet rays and the like, and has a good prevention and control effect on aquatic product pollution caused by vibrio parahaemolyticus. The bacteriophage has a lysis effect on multi-drug-resistant vibrio parahaemolyticus, can replace antibiotics to play a bacteriostatic role, effectively solves the problem of drug resistance faced by current antibiotic treatment, and has relatively good utilization value and application prospect. The bacteriophage can also be prepared into a composition with other bacteriophages to play a synergistic effect, and the bacteriophage can be applied to wider-spectrum antibacterial or bacteriostatic application.

The invention discloses a primer probe composition for detecting prawn disease related pathogens, a kit and application of the primer probe composition. The primer probe composition comprises a first primer probe group used for detecting vibrio parahaemolyticus, a second primer probe group used for detecting a tetrapod iridovirus 1, a third primer probe group used for detecting vibrio vitriocultatus and a fourth primer probe group used for detecting enterocytozoon hepatopenaei. The primer probe composition has the characteristics of high sensitivity, good repeatability and strong specificity, and can be used for specifically detecting vibrio parahaemolyticus, tetrapod iridovirus 1, vibrio vitrioculi and enterocytozoon hepatopenaei.

The invention discloses a nano antibody for detecting vibrio parahaemolyticus as well as a preparation method and application thereof, and aims to provide a nano antibody for detecting vibrio parahaemolyticus, which is high in detection accuracy, strong in specificity and simple in obtaining method. The amino acid sequence of the nano antibody is as shown in SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4 or SEQ ID NO. 5. Belongs to the technical field of biological detection.

The invention belongs to the field of prevention and control of bacterial diseases of aquatic animals, and discloses an acid-resistant broad-spectrum vibrio parahaemolyticus bacteriophage LVPP58 and application of the acid-resistant broad-spectrum vibrio parahaemolyticus bacteriophage LVPP58 in vibrio disease prevention and control, and the preservation number of the bacteriophage is CCTCC M 2025322. The bacteriophage is a broad-spectrum vibrio parahaemolyticus bacteriophage, not only can split vibrio parahaemolyticus, but also can split vibrio alginolyticus, the bacteriophage LVPP58 is stable in titer at the pH of 3-11 and the temperature of 30-60 DEG C, the bacteriophage and antibiotics are combined for use, a synergistic effect can be generated, the treatment effect is enhanced, the occurrence rate of drug resistance is reduced, and a new strategy is provided for solving the problem of bacterial drug resistance.