51 results about "Genital tract" patented technology

The invention discloses a primer probe combination and a kit for detecting genital tract pathogenic microorganisms and application. The primer probe combination comprises primer probe sequences as shown in SEQ ID NO.1-SEQ ID NO.96, the genital tract pathogens comprise 27 pathogens capable of causing genital tract diseases, such as ureaplasma parvum, ureaplasma urealyticum, mycoplasma hominis, mycoplasma genitalium, candida albicans, candida glabrata, candida tropicalis, candida auricula, candida krusei and candida duberlin, as well as lactobacillus crispatus, lactobacillus jensenii and lactobacillus gasseri; the lactobacillus lactis comprises four common vaginal lactobacillus lactis, and also comprises a human-derived interior label. On the basis of a fluorescent quantitative PCR technology, the kit can be used for simultaneously detecting 27 pathogens capable of causing genital tract diseases and 4 common lactobacillus vaginalis in an in-vitro sample, not only can cover main pathogens of genital tract infection, but also meets the evaluation on the ecological environment of the vagina, the detection time is shortened to about 40 minutes, and meanwhile, the kit has relatively good specificity and sensitivity.

The invention discloses a primer combination for identifying six genital tract pathogens and detecting drug resistance genes, a kit and application, the primer combination comprises a primer combination for identifying chlamydia trachomatis, mycoplasma genitalium, ureaplasma urealyticum, mycoplasma hominis, neisseria gonorrhoeae and trichomonas vaginalis and detecting the drug resistance genes of the six genital tract pathogens, and nucleotide sequences are shown as SEQ ID NO.1-SEQ ID NO.50. Microorganisms and related drug-resistant genes in a detection range are identified by utilizing a multiple PCR targeted three-generation sequencing method, the specificity is good, the sensitivity is high, the detection technology has important clinical significance when being applied to identification of genital tract infection pathogens, and compared with a traditional detection method, the detection flux, the coverage range and the sensitivity are higher, and detection is stable; compared with pathogenic microorganism metagenome (mNGS) detection, the method has the advantages that the detection cost is lower, and the detection sensitivity on drug-resistant genes and drug-resistant gene mutation is higher.

The invention provides a kit and a method for simultaneously detecting 11 pathogens of a urogenital system and application, and belongs to the field of molecular biology. The primer group is used for detecting urogenital tract pathogens, and the urogenital tract pathogens comprise ureaplasma urealyticum (UU), chlamydia trachomatis (CT), mycoplasma genitalium (MG), ureaplasma parvum (UP), mycoplasma hominis (MH), adenovirus (ADV), trichomonas vaginalis (TV), herpes simplex virus type I (HSV-I), herpes simplex virus type II (HSV-II), haemophilus influenzae (HI) and neisseria gonorrhoeae (NG). The primer group is used for multi-joint detection of non-gonococcal urethritis related pathogens, has high specificity and sensitivity, is adaptive to non-invasive urine specimens, and supports home self-sampling. The kit is used for clinical diagnosis and can quickly judge pathogen types. Precise medication is performed based on a detection result, the healing time of a patient is shortened, the burden of a doctor is relieved, and meanwhile the pathogen transmission risk is reduced.

The invention relates to lactobacillus gasseri FMTA1121E with an effect of regulating a vaginal micro-ecological environment and application of the lactobacillus gasseri FMTA1121E. The lactobacillus gasseri FMTA1121E is classified and named as lactobacillus gasseri, and the preservation number of the lactobacillus gasseri FMTA1121E is CGMCC No.34962. The lactobacillus gasseri FMTA1121E can be metabolized to generate beneficial components such as lactic acid, gamma-aminobutyric acid and hydrogen peroxide so as to regulate the genital tract environment and improve the health degree; and the bacillus subtilis has excellent bacteriostasis and sterilization effects on various common vaginal pathogenic bacteria such as escherichia coli, staphylococcus aureus, gardnerella vaginalis, candida albicans, neisseria gonorrhoeae and the like. Therefore, the lactobacillus gasseri FMTA1121E strain can be applied to preparation of preparations for preventing and treating related genital tract diseases such as vaginitis.

The invention discloses a kit for rapidly detecting seven genital tract pathogens by a one-tube method. The kit comprises a primer and a probe aiming at ureaplasma urealyticum, a primer and a probe aiming at ureaplasma parvum, a primer and a probe aiming at chlamydia trachomatis, a primer and a probe aiming at gonococcus, a primer and a probe aiming at mycoplasma genitalium, a primer and a probe aiming at mycoplasma hominis and a primer and a probe aiming at trichomonas vaginalis. In addition, the invention also discloses a method for rapidly detecting seven common genital tract pathogens by a one-tube method. According to the method, the detection sensitivity is greatly improved, the operation is simple, and seven genital tract pathogens can be simultaneously detected in a short time.

The invention discloses a primer group and a kit for high-throughput sequencing of targeted genital tract infection pathogens and application of the primer group and the kit, microorganisms and related drug-resistant genes in a detection range are identified by combining multiple PCR (polymerase chain reaction) with high-throughput sequencing, and the sensitivity is high. The detection technology is applied to identification of genital tract infection pathogens and has important clinical significance, the operation steps are simplified, the detection cost is reduced, and the detection efficiency is improved.

The application provides a primer probe composition and application thereof in detection of urogenital tract pathogens, and relates to the technical field of biology. The primer probe composition comprises a chlamydia trachomatis primer probe pair with sequences shown in SEQ ID NO:1-6; a gonococcus primer probe pair with sequences shown in SEQ ID NO:7-9; and a mycoplasma genitalium primer probe pair with sequences shown in SEQ ID NO:10-12. The primer probe pairs in the primer probe composition do not have significant cross interference, the lower limit of detection can be as low as 20 copies / reaction, 200 copies / mL, and the problem that the lower limit of detection of the urogenital tract pathogens in the prior art needs to be reduced is solved.

The invention discloses a primer probe group and a kit for joint detection of mycoplasma genitalium, ureaplasma urealyticum and chlamydia trachomatis, and belongs to the technical field of molecular biological detection. The primer probe group comprises a primer probe group A for directly detecting a mycoplasma genitalium mgpB gene, a primer probe group B for directly detecting a ureaplasma urealyticum ureB gene and a primer probe group C for directly detecting a chlamydia trachomatis ompA gene; the kit comprises a PCR (Polymerase Chain Reaction) reaction solution and the primer probe group. Nucleic acid extraction is not needed, the detection cost is greatly reduced, the experiment time is shortened, meanwhile, the specificity and accuracy are still guaranteed, meanwhile, the sensitivity is improved, and the method has wide application prospects in the fields of non-gonococcal genital tract infection main pathogen detection, infertility and prenatal and postnatal care screening and the like. And the kit has important significance on clinical diagnosis, early treatment and prevention of diseases, prevalence and the like.

In an aspect, a method for applying beta-alanine to a tissue surface is described. The method may comprise applying a composition comprising from about 0.01 % w / w to about 5.0 % w / w beta-alanine to a tissue surface. The beta-alanine may comprise between about 0.08 % w / w to about 2 % w / w. The tissue surface may be suffering from a condition. The tissue surface may be from the group consisting of epidermis, mucosal membrane, respiratory tract lining, urinary tract lining, and reproductive tract lining. In another aspect a composition may comprise from about 0.01 % w / w to about 5.0 % w / w beta-alanine; water; an emulsifying component; and a fat. The fat may be an emollient or a moisturizing component.

The invention belongs to the technical field of medical supplies, and particularly relates to an adjustable cervix uteri multi-point micro-injection device which comprises a guide cylinder, an adjusting mechanism is mounted in the guide cylinder, and an injection needle is mounted on the side, extending into a human body, of the adjusting mechanism; the other side of the adjusting mechanism is connected with the carry control mechanism, the rotation control mechanism and the deflection control mechanism through a first connecting rod, a second connecting rod and a third connecting rod. The other side of the adjusting mechanism is further connected with a connecting hose, and the other end of the connecting hose is connected with an injector. Wherein the adjusting mechanism comprises a carry mechanism, a rotating mechanism and a deflection mechanism, the situation that the device pierces other parts of the genital tract of a patient to cause injury before reaching the cervical lesion part can be avoided, and the injection needle can be deflected towards different parts through the adjusting mechanism; according to the device, the injection needle can accurately pierce a diseased region for injection under the condition that the adjustment range in the genital tract of a patient is minimum.

The invention relates to the technical field of livestock artificial insemination, in particular to a pig artificial insemination insemination insemination device which comprises a U-shaped handle frame, the upper end of the U-shaped handle frame is provided with a horizontally telescopic folding air bag, and the front end and the rear end of the folding air bag are provided with a valve body and a semen output control mechanism respectively. The front end and the rear end of the folding air bag are symmetrically provided with hard cover bodies. By arranging the folding air bag, the valve body and the semen discharging control mechanism, the semen discharging control mechanism can be used for accurately controlling contraction of the folding air bag, semen is effectively conveyed into the uterus of the sow, and sufficient conveying of the semen and the success rate of artificial insemination are ensured; the rotating ring seat and the second semen outlet can be discontinuously communicated with the first semen outlet in the contraction process of the folding air bag, then the continuous circulation state of semen in the conveying process is achieved, the impact force of the semen is enhanced, and the semen can more deeply enter the genital tract of the sow.

The invention belongs to the technical field of medicines, and discloses a gynecological bacteriostatic lotion and a preparation method and application thereof.The lotion is prepared from extracts of traditional Chinese medicinal materials such as radix sophorae flavescentis, cortex phellodendri, coptis chinensis, scutellaria baicalensis, rhizoma smilacis glabrae, cortex dictamni, fructus cnidii, areca nuts, cortex meliae, golden larch bark, lithospermum erythrorhizon, lavender, rheum officinale and honeysuckle as main raw materials, and parachloroxylenol, salicylic acid and borneol as auxiliary materials. A compound preparation with multiple effects is formed through scientific proportioning and a preparation process, the product can effectively inhibit common pathogenic bacteria such as Candida albicans, Staphylococcus aureus and Escherichia coli, shows a broad-spectrum and efficient antibacterial effect, and is small in irritation, high in safety to vaginal mucosa and genital tract environment and free of toxic and side effects. The product can maintain local micro-ecological balance and reduce inflammation recurrence while cleaning and nursing, has the advantages of long-term conditioning of a traditional Chinese medicine compound and rapid sterilization of western medicine components, and is suitable for clinical popularization and application.

The invention discloses a vaginal balance peptide derived from a vaginal microbiome and application thereof, the vaginal balance peptide can selectively inhibit lower genital tract infection pathogens, has no inhibition effect on vaginal probiotic lactobacillus, and has: (a) an amino acid sequence as shown in SEQ ID NO.1; or (b) an amino acid sequence which is obtained by substitution, deletion and / or addition of one or more amino acids of the amino acid sequence as shown in SEQ ID NO.1 and has the same function as that of SEQ ID NO.1; or (c) an amino acid sequence which has at least 85% homology with the amino acid sequence shown in SEQ ID NO.1 and has the same function as the amino acid sequence shown in SEQ ID NO.1. The balance peptide is efficient in antibiosis, specifically inhibits growth of lower genital tract infection pathogens, and has a remarkable inhibiting effect on gardnerella vaginalis, group B streptococcus, candida albicans and the like; the selectivity is high, the effect of inhibiting growth of various common vaginal probiotics lactobacillus is avoided, and the effect of balancing micro-ecology is achieved; the safety and the treatment effect are good.

The utility model relates to the technical field of animal husbandry instruments, in particular to a genital tract effusion collecting device for livestock in animal husbandry. The genital tract effusion collection device comprises an extraction part, a suction catheter used for stretching into the effusion position and a support assembly. The extraction part is provided with a storage cylinder used for containing genital tract effusion. And a piston is arranged in the storage cylinder. A connecting column is arranged at the bottom of the storage cylinder. And a suction channel is arranged in the connecting column. The side wall of the connecting column is sleeved with the suction catheter. The suction catheter is connected with the storage cylinder through the suction channel. The support assembly comprises a lantern ring and supporting legs. And the lantern ring is mounted on the side wall of the storage barrel. And the plurality of supporting legs are circularly arranged along the axis of the lantern ring. The supporting legs are connected with the side wall of the lantern ring. According to the genital tract effusion collecting device for the livestock in the animal husbandry, collection of the genital tract effusion of the livestock is achieved, the safety is improved, the difficulty of effusion extraction is reduced, and the collecting efficiency is improved.

The application discloses a primer and probe for urogenital tract microorganism detection, a kit and a use method of the kit. The primer and probe for urogenital tract microorganism detection, the kit and the use method of the kit can simultaneously detect chlamydia trachomatis, ureaplasma urealyticum and gonococcus. By using real-time fluorescent PCR amplification technology, only one nucleic acid extraction and one-step PCR detection reaction are needed. The use method of the kit is rapid, simple, specific, high in sensitivity and wide in detection range. The sensitivity can reach 400 copies / ml, the detection range is 400 copies / ml to 4.00E+09 copies / ml, and the non-chlamydia trachomatis, ureaplasma urealyticum and gonococcus pathogenic microorganisms cannot be detected, so the problem of false positive is not prone to occur.

A microbiome modulating composition and a method for producing the microbiome modulating composition are described herein. The method comprises preparing a culture from at least two of any Lactobacillus spp. bacteria, Limosilactobacillus spp. bacteria, or combinations thereof, passaging the culture on a glycogen carbon source to form a postbiotic ferment of the at least two of any Lactobacillus spp. bacteria, Limosilactobacillus spp. bacteria, or combinations thereof, and adding the postbiotic ferment to a carrier. Further disclosed is a method for treating microbial dysbiosis of an urogenital area or a skin area of a user, the method comprising applying the microbiome modulating composition to an urogenital or a skin area of a user. The disclosed methods and microbiome modulating compositions help promote the growth of healthy Lactobacillus spp. bacteria to prevent dysbiosis and other related conditions and infections.

This invention provides a composition for detecting genital tract pathogens, its preparation method, and its application, belonging to the field of nucleic acid or microbial assays or tests. The composition provided by this invention includes a forward primer, a reverse primer, and a probe targeting seven genital tract pathogens. The forward and reverse primers are used to amplify the target DNA. A blocking group is added between the i-th nucleotide and the (i+1)-th nucleotide from the 3' end of the reverse primer, where 4 ≤ i ≤ 8. The probe is labeled with a fluorescent group and a second quenching group at both ends, and a first quenching group in the middle. A portion of the probe's sequence is identical to a portion of the reverse primer's sequence. The composition provided by this invention enables multiplex qualitative detection of seven genital tract pathogens: Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma urealyticum, Ureaplasma microsporum, Mycoplasma hominis, Mycoplasma genitalium, and herpes simplex virus type 2.

The application discloses a multiplex detection kit for urogenital tract infection pathogens, which can simultaneously and efficiently and accurately detect at least two of four pathogens, CT, MG, MH and NG, the minimum detection limit of the four pathogens is 400 copies / mL, and the kit is perfectly compatible with three samples, namely, non-invasive urine, male urethral secretions and female cervical secretions, and has a wide application range.

The invention discloses a gynecological assisted reproduction flushing device, which belongs to the field of medical auxiliary equipment, and comprises a base, a water collecting cylinder, an inflation tube, a guide head and a water spraying ring, a plurality of flushing holes distributed in an annular array are formed in one end, close to the base, of the water spraying ring, and a water diversion cavity is formed in the base; the water diversion cavity and the flushing holes are communicated through a plurality of liquid conveying pipes fixedly installed on the inner side of the water collecting barrel, a liquid inlet pipe is fixedly installed at the end, away from the water collecting barrel, of the base, the liquid inlet pipe is communicated with the water diversion cavity, and the function of flushing from the inner side to the outer side is achieved by designing a water spraying ring and the flushing holes distributed in an annular array mode; the flushing holes are obliquely formed in the direction away from the center point of the water spraying ring, it is guaranteed that a certain included angle is kept between flushing fluid and the genital tract wall, and therefore all corners of the inner wall of the genital tract can be fully flushed, the flushing uniformity and thoroughness are improved, and discomfort and injuries caused by the fact that the flushing fluid directly impacts the genital tract wall are avoided.

Lower genital tract access devices, methods, systems and kits Disclosed are devices, methods, systems and kits for obtaining biological tissue samples. Also disclosed are hysteroscopy or colposcopy devices that eliminate the need for a separate speculum and that improves patient comfort.

The present invention relates to a composition, especially to a pharmaceutical composition, for intravesical application into the urinary bladder, preferably via instillation by means of a catheterizing device), particularly for use in the prophylactic and / or curative treatment of diseases and / or dysfunctions of the urogenital tract, preferably diseases and / or dysfunctions of the urinary bladder.

The invention discloses a genital tract sample self-sampling suite which comprises a sleeve, the sleeve is in a cylindrical shape with two open ends, threaded grooves are formed in the outer side walls of the top and the bottom of the sleeve, the top of the sleeve is in threaded connection with a sealing cover, and the sealing cover is connected with a connecting rod through a lifting device. A baffle block is fixedly connected to the side wall of the connecting rod, a swab is fixedly connected to one end of the connecting rod, a storage head is arranged at the bottom end of the sleeve, a film is connected between the sleeve and the storage head through a clamping device, and a sample storage device is arranged in the storage head. The genital tract self-sampling device is suitable for self-sampling of a genital tract, is convenient to operate during self-sampling of the genital tract, facilitates self-sampling of a patient and reduces fear, meanwhile, collected samples can be transported without being completely dried through the cell preservation liquid or the drying agent, the samples cannot be cracked, are well preserved and are kept in a good state before an experiment, and the retention time is long.

Lower genital tract access devices, methods, systems and kits Disclosed are devices, methods, systems and kits for obtaining biological tissue samples. Also disclosed are hysteroscopy or colposcopy devices that eliminate the need for a separate speculum and that improves patient comfort.

The invention discloses application of double-cavity probiotic intervention in colon cancer chemotherapy and intestinal tract and genital tract flora, and relates to the technical field of biological medicine. The bifidobacterium longum subsp. Longum NCU-05 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on January 18, 2023, and the preservation number is CGMCC NO.26492; the lactobacillus crispatus NCU-28 is preserved in the China General Microbiological Culture Collection Center on July 22, 2024, and the preservation number of the lactobacillus crispatus NCU-28 is CGMCC NO.31387. The lactobacillus crispatus NCU-28 has been preserved in the China General Microbiological Culture Collection Center. The traditional Chinese medicine composition has the beneficial effects that intestinal tract and genital tract flora imbalance after colon cancer chemotherapy can be improved, and the traditional Chinese medicine composition can be used as an auxiliary medicine for treating colon cancer. The bifidobacterium longum subsp. Longum and the lactobacillus crispatus are administered through a bidirectional crosstalk cavity of intestinal-vaginal microbiota in a combined medication form, so that the intestinal tract and genital tract dysbacteriosis after colon cancer chemotherapy is effectively improved.

The present invention discloses a three-axis motion sampling mechanism, which includes a frame, a multi-axis motion unit, the multi-axis motion unit is arranged inside the frame and is composed of an X-axis motion mechanism, a Y-axis motion mechanism and a Z-axis motion mechanism; a cantilever support mechanism, the cantilever support mechanism is arranged on the frame; a sampling part, the sampling part is arranged on the Z-axis motion mechanism and is used for sampling the lower sample; wherein, the Y-axis motion mechanism is of a cantilever type, and the cantilever support mechanism is arranged at the cantilever end of the Y-axis motion mechanism and is used for supporting the Y-axis motion mechanism. The advantages of the present invention are that the yaw amount of the cantilever end is greatly reduced, so that the sampling part can perform sampling work quickly and stably; during the sampling process, under the action of the force when the sampling part rises, the lower support bearing will contact the lower surface of the support bar, and at the same time react on the lower sampling part, ensuring that the Z direction does not deform due to the cantilever during sampling, and ensuring that the sampling process is carried out quickly and stably.

This invention provides a dual-segment, dual-color pH comparison catheter for clinical identification of premature rupture of membranes, belonging to the field of medical catheter technology. It includes a main catheter tube with a urine segment pH membrane and an amniotic fluid segment pH membrane sequentially arranged along the axial direction on the anterior outer wall of the main tube. The urine segment pH membrane has a color development range of pH 5.5-6.5, and its color change is a continuous gradient from yellow to green. This invention achieves zero-sampling detection under sterile conditions, highly integrating pH identification function into routine catheterization procedures. The pH value of urine and any potentially mixed amniotic fluid is simultaneously detected upon catheter insertion into the bladder, without the need for vaginal speculum, sampling swabs, or any form of secondary sterile opening. This design completely blocks the path of exogenous bacteria into the maternal reproductive tract at a physical level, thereby fundamentally and effectively reducing the incidence of clinically common chorioamnionitis and urinary tract infections, and improving perinatal medical safety and quality.

This invention relates to the field of in vitro diagnostics, and particularly to a combined diagnostic kit for prostate diseases. The invention provides reagents including pH, peroxidase, elastase, lecithin bodies, citric acid, zinc, and acid phosphatase under mild reaction conditions, enabling rapid differentiation of inflammatory and non-inflammatory prostatitis, benign prostatic hyperplasia, and non-prostatic genital tract infections using a single test card. Through extensive experimentation, this invention has developed a one-step reaction scheme suitable for the dry chemical determination of acid phosphatase and lecithin bodies in seminal plasma. This method eliminates the need for a stop solution, achieving a one-step reaction under mild conditions that will not cause damage to operators or fully automated instruments. The samples used are seminal plasma or semen, allowing for large sample volumes and easy acquisition, significantly improving diagnostic efficiency for doctors and patient comfort.

The invention discloses a control method for a liquid path system of a genital tract secretion analyzer. The control method comprises the following steps: 1, confirming the pure water amount of a water feeding unit, the reagent amount in a first container and the waste liquid amount of a waste liquid unit; then filling a water feeding unit and a reagent, and quantitatively adding a sample diluent into the sample by utilizing a reagent filling unit to obtain a diluted sample; the sample filling unit is used for taking a TIP head and quantitatively sucking and diluting a sample into a board card and a reaction container for several times; quantitatively filling a reaction reagent into the board card by using a reagent filling unit, and carrying out dry chemical detection; a reagent filling unit is used for quantitatively filling staining fluid into the reaction container, and the staining fluid is dropwise added to a slide for morphological detection after staining. The sample filling unit adopts a sample pump for direct filling, and the reagent filling unit adopts positive pressure filling, so that only one TIP head is needed in the detection process of each sample, on one hand, the TIP head material consumption is remarkably reduced, on the other hand, the action logic of sample pretreatment is simplified, and the detection efficiency of an instrument is improved.

The artificial insemination transplantation tool comprises a propelling assembly and a protection assembly which are arranged on a pipetting cylinder, the propelling assembly comprises a liquid storage bottle arranged at one end of the pipetting cylinder, the front end of the liquid storage bottle is embedded into the pipetting cylinder, an infusion hose is arranged in the pipetting cylinder, and a piston baffle is arranged at the end, close to the liquid storage bottle, of the infusion hose; the piston baffle and the pipetting cylinder form a first closed cavity; the protection assembly comprises a second closed cavity formed in the end of the pipetting barrel, the second closed cavity is communicated with the first closed cavity through a ventilation pipe, the peripheral side face of the end of the pipetting barrel is sleeved with an elastic air bag, the circumferential inner side of the elastic air bag is provided with a plurality of one-way air valves, and the end of the pipetting barrel is hinged to a connecting part arc-shaped cover plate. The infusion hose is protected through the arc-shaped cover plate, the infusion hose is effectively prevented from touching the inner wall of the genital tract, the prevention effect of gynecological diseases is improved, the infusion hose is arranged in the pipetting cylinder, the infusion hose can enter the cervix uteri more easily, and the artificial insemination success rate is effectively increased.