105 results about "Microecology" patented technology

The invention discloses a composition for regulating scalp micro-ecology and application thereof, and relates to the technical field of daily chemical products. The composition for regulating scalp micro-ecology comprises the following components: emu oil, melaleuca alternifolia leaf oil, Brazil green propolis and hydrogenated castor oil. The invention provides a composition for regulating scalp microecology and application thereof, the composition is prepared by compounding an anti-inflammatory and antibacterial component emu oil with melaleuca alternifolia leaf oil, Brazilian green propolis and hydrogenated castor oil, the composition has synergistic bacteriostasis and anti-inflammation effects, keeps scalp microbe balance, reduces red and swollen skin and itching, and also can promote the regeneration capacity of skin tissue cells.

The invention belongs to the technical field of microorganisms, and particularly relates to combined lactobacillus salivarius and application thereof in female genital tract health. The preservation number of the strain is CCTCC NO: M20231719, and the strain has good bacteriostatic ability and dendritic cell (DC) maturation / activation stimulation ability, has no potential safety problem, can regulate vaginal micro-ecological balance, prevent infection caused by exogenous bacteria, effectively improve tumor immunosuppression microenvironment, and has good application prospects. And the method has very important significance on prevention / treatment of vaginal dysbacteriosis patients, gynecological tumors and precancerous lesions thereof.

The invention belongs to the technical field of bioengineering and microbiomics, and particularly relates to an acquisition method and application of a specific metabolic substrate of lactobacillus crispatus and application of a specific carbohydrate active enzyme CAZzyme gene and a polysaccharide utilization site (PUL). Genomes of lactobacillus crispatus and lactobacillus inertus are obtained from a public database, gene annotation is carried out on the genomes of the lactobacillus crispatus and the lactobacillus inertus, and a random forest model and a KEGG metabolic pathway diagram are combined to find specific CAZzyme genes, PUL and other genes with metabolic functions of the lactobacillus crispatus; a substrate which only lactobacillus crispatus can metabolize is searched based on the genome difference of the two bacteria, and the ability of the obtained substrate for promoting the growth of lactobacillus crispatus and inhibiting the growth of inert lactobacillus is evaluated through experiments. Growth and reproduction of the lactobacillus crispatus are promoted through the substrates, so that the lactobacillus crispatus accounts for a higher proportion in vagina microecology, the vagina is protected, and reproduction of other unfavorable bacteria is reduced. The assisted reproduction success rate is improved.

The invention discloses a composite fiber material containing Miao medicine extract and bioactive polypeptide and application of the composite fiber material in preparation of underwear, and relates to the technical field of functional textile materials. Comprising a metabolite response type core layer, a flora adaptive type sheath layer and a dual-mechanism sensing release interface arranged on the surface of the core layer, according to the invention, on the basis of a core-sheath structure and a dual-mechanism sensing interface, the composite fiber can accurately respond to skin micro-ecology, directionally release Miao medicine extract and bioactive polypeptide, protect beneficial bacteria, inhibit harmful bacteria and prevent unbalance, and can also form a component-micro-ecology-skin collaborative closed loop; micro-ecology visualization is achieved through an AI E probe, washing resistance is achieved, the function is stable, the physical performance is matched with underwear, prebiotics are supplemented in the preparation process, the health value can be enhanced, sensitive skin is matched, and the discomfort risk is reduced.

The invention relates to the technical field of cut flower postharvest preservation, and discloses a preservation pre-treatment solution for maintaining moisture-nutrition-microecological balance in a storage period of babysbreath cut flowers and a synergistic application method thereof. The pre-treatment liquid is prepared from the following components in percentage by weight / volume: 15 to 30 g / L of cane sugar, 15 to 30 g / L of trehalose, 100 to 200 mg / L of 8-hydroxyquinoline citrate, 50 to 150 mg / L of citric acid, 50 to 100 mg / L of carboxymethyl chitosan, 0.3 to 1.0 g / L of lysozyme-chitosan compound, 5 to 15 mg / L of nano silver-loaded silicon dioxide, 0.1 to 1.0 g / L of manganese citrate and 0.5 to 1.0 g / L of calcium nitrate. And 5 to 10 mL / L of glycerol and 0.5 to 5.0 mL / L of Tween-20 in percentage by volume. Through component collaborative design-process precise control-package matching innovation, the bottleneck of double loss of water and nutrition in the storage period is broken through, the pain points of no flowering and gray mold outbreak after storage are solved, a whole-process nursing scheme is provided for long-distance and short-distance transportation and storage of the babysbreath cut flowers, the commodity rate of the cut flowers is remarkably increased, and the economic benefit is increased. The method is suitable for flower harvesting bases, cold chain transportation and wholesale markets, and is especially suitable for air transportation exits.

The invention provides the food calling type river crab feed, the application effect of the food calling type river crab feed in river crab culture shows that the food calling type river crab feed has remarkable advantages in the aspects of improving the growth performance, enhancing the immune function, optimizing the intestinal microecology, improving the water quality environment and the like, the use of the fish meal is reduced through the fermented vegetable protein, and the specific composite phagostimulant has the advantages that the utilization rate of the fish meal is reduced; the palatability of the river crab feed with the fish meal content reduced is improved, the feed coefficient is reduced, the unit economic benefit is improved, the problem of environmental pollution in the breeding process is effectively relieved, and the river crab feed has good ecological and economic dual values.

The invention discloses a composition for improving immunity and delaying senescence and a preparation method thereof. Belongs to the technical field of functional health. The composition comprises the following components in parts by mass: 5-50 parts of collagen, 10-80 parts of paeonia ostii tea, 1-20 parts of an antioxidant, 3-10 parts of an intestinal microecology regulator and 1-3 parts of an energy metabolism and nerve regulator. According to the invention, through multi-system cooperation and multi-target intervention, dual effects of immune enhancement and senescence delay are realized. Through scientific compounding and a special process, the product has the advantages of stable components, uniform activity and good solubility. Animal experiments and theoretical models show that immune organs can be effectively protected, accumulation of aging markers is reduced, and a solution is provided for development of comprehensive anti-aging health products.

The invention discloses an application of a prebiotic component in enhancing athletic ability and improving athletic performance. The prebiotic component is xylooligosaccharide. Animal experiments show that mice given with xylooligosaccharide can significantly improve the abundance of probiotics and enhance the muscle strength, constant-speed endurance and other movement functions of the mice. The xylooligosaccharide serving as an efficient prebiotic can be specifically utilized by specific probiotics in intestinal tracts, the abundance of the xylooligosaccharide is remarkably improved, growth of pathogenic bacteria is inhibited, then the micro-ecological environment of the intestinal tracts is optimized, and the exercise function improvement phenomena such as increase of muscle strength and delay of fatigue in endurance exercise are shown in behavioral tests. The cross-organ integration regulation and control from the intestinal microecology to the whole body physiological state and then to the athletic performance is reflected, so that the xylooligosaccharide has important significance in enhancing the athletic ability and improving the athletic performance.

The invention provides application of beta-1, 4-glucan type cellooligosaccharide in improvement of plant rhizosphere micro-ecology and enhancement of IAA signals, and belongs to the technical field of biological agriculture. The invention provides an application of beta-1, 4-glucan type cellooligosaccharide with the polymerization degree of DP2-DP5 in improvement of plant rhizosphere micro-ecology and enhancement of IAA (Indole Acetic Acid) signals. In the invention, beta-1, 4-glucan type cellooligosaccharide can induce plant immunity in a growth allowable PTI threshold range, so that a rhizosphere microflora structure is directionally remodeled, the relative abundance of beneficial microorganisms is improved, the potential pathogen load is reduced, rhizosphere IAA and related metabolites thereof can be increased, and'disease resistance without growth inhibition 'is realized. The invention further provides an application of the beta-1, 4-glucan type cellooligosaccharide combined with rhizosphere beneficial microorganisms in biological agriculture, and growth promotion and / or nodulation nitrogen fixation effects can be enhanced.

The invention discloses a method and system for synchronously detecting host chromatin openness and in-vivo microbiome based on transposase, and belongs to the technical field of biological sequencing data analysis. According to the method, transposase is used for selectively fragmenting an open chromatin region of a host, and a microbial genome is almost randomly cut, so that synchronous enrichment of host and microbial DNA is realized; after high-throughput sequencing library construction and double-end sequencing, sequencing data is split into host source and non-host source reads through bioinformatics analysis, host chromatin state and microorganism composition are analyzed respectively, and a microorganism-host epigenetic regulation network is constructed; the invention further provides a matched DNA sequencing library and an analysis system, multi-scene research of infectious diseases, intestinal microecology, tumor microenvironment and the like is supported, a public database can be reanalyzed, and potential microbial interaction signals are mined. According to the method, the host-microorganism interaction research efficiency is remarkably improved, and a high-sensitivity and integrated technical scheme is provided for epigenetic regulation mechanism analysis.

This invention discloses a method for the synergistic metabolic analysis of multiple components of traditional Chinese medicine (TCM) integrated with the gut microbiota. After initializing the microbiota ecosystem, a solution of multi-component TCM extracts is continuously injected into a microfluidic chip. Stable isotope-labeled precursors are pulsed into a specific simulation region to perform spatial isotope encoding. A standing wave acoustic field is applied at the sampling node of the microfluidic chip, and multiphase fluid spatial splitting is performed using the difference in the primary acoustic radiation force experienced by the particles to derive cell-free TCM metabolites. The cell-free TCM metabolites are separated by chromatography and gas phase drift separation. A four-dimensional metabolic feature array is constructed, and the absolute value of the mass shift is calculated for spatial inverse decoding. Closed-loop feedback is triggered based on the rate of change of target metabolite ion abundance, increasing the amplitude of the standing wave acoustic field pressure to confine intestinal bacteria and performing in-situ enrichment of synergistic metabolic intermediates. This invention realizes the spatial metabolic trajectory tracing and network reconstruction of multiple components of TCM in a complex gut microbiota ecosystem.

The invention discloses high-elasticity functional tofu and a preparation method thereof in the technical field of tofu processing, the high-elasticity functional tofu is prepared by adding dictyophora indusiata polysaccharide during soybean milk curdling, and the dictyophora indusiata polysaccharide has multiple biological activities such as inflammation resistance, oxidation resistance and intestinal microecology regulation, so that the health-care function of the tofu is improved; the dictyophora indusiata polysaccharide is obtained by preparing dried powder by taking fresh dictyophora indusiata as a raw material and extracting the dried powder by any one of a hot water extraction method, an ultrasonic extraction method and an enzyme extraction method. Through interaction of the bamboo fungus polysaccharide and protein in the bean curd gel, the texture quality of the bean curd is improved, and the bean curd is good in elasticity, moderate in hardness and good in chewing taste.

This invention discloses a strain of *Lactobacillus paracasei* CCFM1333 with multi-target regulatory effects and its application in regulating oral microecology, belonging to the field of microbial technology. This invention yields a strain of *Lactobacillus paracasei* CCFM1333, whose fermented milk and inactivated fermented milk not only inhibit odor-causing bacteria in the oral cavity and regulate oral microecology, but also alleviate inflammation. Specifically, it can reduce the number of odor-causing bacteria in the oral cavity of rats with increased odor-causing bacteria; downregulate the levels of serum inflammatory factors IL1β, IL-6, and IL-17A; and reduce the relative abundance of pathogenic bacteria in the oral cavity of rats, effectively regulating the oral microecology of rats.

The application discloses a brain hemorrhage prognosis improvement method based on intestinal flora regulation and relates to the technical field of intestinal microecology.The application comprises the following steps: detecting feces, starting intervention if the enterococcus abundance and neuroinflammatory metabolic markers exceed the standard, stopping the use of conventional nutrient solution, perfusing a special component containing sugar analogs, blocking the energy uptake of enterococcus, simultaneously providing a carbon source for beneficial bacteria, synchronously perfusing a microsphere suspension containing a molecular capture function, adsorbing and fixing proinflammatory metabolic waste in the intestinal tract, blocking the blood entry of the proinflammatory metabolic waste, perfusing live bacteria with different functions three times in time sequence, sequentially completing the acidification of the environment, constructing a bacterial membrane barrier and repairing the intestinal epithelium, and re-detecting after each round of intervention, selecting the maintenance period or adjusting the intensity and then recycling according to the decrease of enterococcus and markers.The application can limit the competitive advantage of enterococcus in the utilization of carbon sources after brain hemorrhage by sequentially implementing competitive inhibition of the phosphotransferase system and multi-species ecological niche occupation.

The invention discloses a strain of chicken source lactobacillus casei H0001 and application of the chicken source lactobacillus casei H0001 in improvement of intestinal microecology of chicks. The chicken source lactobacillus casei provided by the invention is clear in source, high in host adaptability and suitable for colonization in the intestinal tract of a chick in the early stage; the strain provided by the invention can significantly improve the diversity of intestinal flora and the abundance of beneficial bacteria of chicks, and improve the intestinal micro-ecological structure; according to the invention, a safe and effective micro-ecological technical means is provided for early intestinal health regulation and control of chicks to replace or reduce the use of antibiotics; and (4) the method has a good application prospect and can be used for green and healthy breeding of poultry.

The utility model discloses a sampling capsule for in-situ sampling of a digestive tract. The sampling capsule comprises a capsule shell, a sample cabin door is formed in the capsule shell, a sample cabin, a circuit board and a battery are arranged in the capsule shell, the sample cabin corresponds to the sample cabin door in position, the circuit board is electrically connected with a micro motor, the output end of the micro motor is connected with a gear set, and the gear set is used for amplifying the acting force of the micro motor. The gear set is connected with a sample cabin door on the capsule shell through a transmission shaft; the gear set comprises a driving gear connected with the output end of the micro motor, a driven duplicate gear connected with the driving gear in a meshed mode and a driven gear connected with the driven duplicate gear in a meshed mode. The device can prevent proteins, metabolites and the like from being re-absorbed by colon, can effectively reflect the structures of biome, proteome and metabolome at each stage of the digestive tract, and provides a new device for research on space-time structure of microecology of the digestive tract and composition change of the space-time structure during occurrence and development of diseases.

This application provides a *Lactobacillus curvatureii* bacteriocin, its preparation method, and its application, relating to the field of biotechnology. The *Lactobacillus curvatureii* bacteriocin provided in this application is obtained through gene recombination expression and is a natural antibacterial ingredient with a broader antibacterial spectrum, low irritation, and long-lasting effect. Applying this *Lactobacillus curvatureii* bacteriocin to feminine hygiene products not only enhances the product's antibacterial effect but also helps repair and maintain the balance of the microecology, preventing disruption of normal flora, effectively maintaining the acidic environment of the intimate area, inhibiting the growth of pathogenic bacteria, and reducing the risk of infection. The feminine hygiene product of this application combines gentleness, high efficacy, and multifunctionality, while also focusing on microecological balance and mucosal repair to meet the higher needs of women's intimate care.

This invention provides the application of a *Breago brevis* strain in the preparation of products that promote intramuscular fat deposition in livestock and poultry. The *Breago brevis* strain is *Breago brevis* JCM 32280. Under high-fiber dietary conditions, supplementation with *Breago brevis* JCM 32280 of this invention can efficiently degrade fiber to produce high concentrations of butyrate. Butyrate, as a key effector molecule, can directly bind to and activate PPARγ receptors in host cells, thereby upregulating the MOGAT2-mediated triglyceride synthesis pathway, significantly increasing body fat percentage in mammals and promoting intramuscular fat (marbling). Simultaneously, this strain can reshape the intestinal microecology, specifically increasing the abundance of beneficial intestinal lactobacilli and enhancing the intestinal mucus barrier, providing a safe and efficient microbial preparation strategy for improving the fat deposition capacity and meat flavor of livestock and poultry.

The invention belongs to the technical field of probiotics, and particularly relates to plant lactobacillus with bacteriostatic activity and application of the plant lactobacillus. Wherein the plant lactobacillus with bacteriostatic activity is separated from the intestinal tract of a healthy human body, and the LV1000 fermentation filtrate freeze-dried powder is prepared through fermentation, centrifugation, filtration, ultrafiltration concentration and vacuum freeze-drying by taking inactivated staphylococcus epidermidis bacterial suspension as an inducer through a specific induction fermentation culture medium. Meanwhile, the freeze-dried powder is compounded with an embedding compound, galactooligosaccharide, beta-cyclodextrine, erythritol and the like, so that the fermented beverage capable of improving the intestinal microecology is prepared. The freeze-dried powder of the LV1000 fermentation filtrate can specifically inhibit enteropathogenic bacteria, the tea polyphenol in the embedded compound can further synergistically enhance the bacteriostatic action and exert the antioxidant effect, and the fermented beverage which is high in stability, fine and smooth in taste, free of refrigeration and capable of being directly drunk and improving the intestinal microecology is prepared.

The invention relates to the technical field of fertilizers, and discloses a biological protein principle-based fertilizer for preventing and treating citrus liberobacter asiaticum, which is prepared from the following raw materials in parts by weight: 40 to 70 parts of fish protein peptide fermentation products, 5 to 15 parts of EM flora active liquid, 1 to 5 parts of chelated trace element solution and 10 to 20 parts of algal polysaccharide-polyglutamic acid compound. The fish protein peptide is fermented for 180 days or longer through EM bacteria. The EM flora active liquid contains lactic acid bacteria, photosynthetic bacteria, actinomycetes and saccharomycetes. The trace elements are at least two of boron, zinc, iron, manganese and molybdenum and are chelated by polyglutamic acid or sugar alcohol. The small peptide, the bacterial metabolite and the chelate element are in slow release synergy, so that rhizosphere microecology can be rebuilt, defensive enzymes can be induced, and the phenomena of huanglong, yellowing and red nose can be relieved.

The invention discloses an application of a prebiotic component in promoting muscle growth, enhancing muscle strength and improving exercise tolerance. The prebiotic component is galactooligosaccharide. Animal experiments show that mice given with galactooligosaccharide show motor function improvement phenomena such as muscle strength increase and fatigue delay in endurance movement in behavioral tests, and the phenomenon that fast muscle and slow muscle fiber components are increased at the same time; the cross-organ integration regulation effect from the intestinal microecology to the whole body physiological state and then to the athletic performance is reflected.

The invention discloses streptomyces alboflavus (with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.35588) for preventing and treating muskmelon fusarium wilt, and belongs to the field of biological prevention and treatment. The strain is high in sporulation quantity and strong in colonization capacity, pathogenic bacteria are inhibited and growth is promoted through antagonism, secondary metabolites, induction system resistance and other mechanisms, the potting control effect reaches 86.3%, and the effect lasts for 21 days. The streptomyces albidoflavus not only can prevent and treat fusarium wilt of muskmelons, but also has a broad-spectrum inhibition effect on fusarium wilt of cucumbers, watermelons and tomatoes and soil-borne diseases such as pepper phytophthora blight and rhizoctonia solani, and can improve rhizosphere microecology and promote reproduction of effective microbial communities. The biocontrol microbial agent is a pure biological agent, is pollution-free and nuisanceless, and can remarkably reduce the use of chemical pesticides, relieve continuous cropping obstacles and promote green development of the watermelon and melon industry.

This invention provides a composition comprising milk thistle extract and Akkella myxophilus. This composition can rapidly increase the abundance of Akkella myxophilus in the intestinal environment, promote its proliferation in the intestine, increase the variety and abundance of beneficial intestinal bacteria, colonize beneficial bacteria, and eliminate harmful bacteria. It has the effects of improving intestinal microecology, relieving fatigue, protecting the liver, reducing fat, relieving hangovers, anti-inflammatory, antioxidant, anti-aging, and beautifying the skin.

The invention relates to the technical field of animal feed, and discloses rabbit feed and a preparation method thereof. The rabbit food comprises a particle main body, wherein the particle main body comprises a plant raw material and a first part enzyme-fiber complex; the coating layer coats at least part of the surface of the particle main body, and the coating layer comprises a second part enzyme-fiber complex, an active functional dry powder mixture, a binder and a free-flowing agent; wherein the enzyme-fiber complex comprises a carrier and an enzyme preparation loaded on the carrier, and the active functional dry powder mixture comprises probiotics, metabiotics and essential oil microcapsules. The inner layer of the rabbit food disclosed by the invention firstly plays a preliminary hydrolysis role in a gastric acid environment, and the outer layer is released in the middle section of an intestinal tract so as to regulate micro-ecology and inhibit harmful bacteria; and the free-flowing agent and the binder improve the fluidity and anti-shedding performance, and the particle-coating structure realizes targeted controlled release and segmented effect of the active ingredients at different parts of the digestive tract, so that the digestibility and health-care effect of the feed are improved.

The invention discloses a ternary whitening and skin nourishing composition as well as a preparation method and application thereof. According to the composition, a radix paeoniae alba, rhizoma atractylodis macrocephalae and poria cocos Sanbai decoction composition and liquorice are extracted and purified according to a specific proportion, Sanbai decoction nanoparticles are prepared through a chitosan-TPP ionic gel method, and efficient delivery of active ingredients is ensured; litchi is subjected to probiotic fermentation, litchi fermentation liquor is mixed with nanoparticles, the viable count is increased through secondary co-fermentation, and short-chain fatty acid and active peptide are generated; finally, calcium alginate-gamma-PGA double-layer network microspheres are formed in one step through an atomization-ionic gel method, and synchronous encapsulation of viable bacteria and plant active ingredients is achieved. Litchi polyphenol, Sanbai decoction aglycone and probiotic metabolites construct a'plant-micro-ecological-host 'three-dimensional whitening network, and tyrosinase activity and melanin generation are remarkably inhibited.

The invention belongs to the technical field of compound microorganism compositions, and particularly relates to a female health conditioning composition based on Lactobacillus complex flora and a medicinal fungus extract, the composition comprises compound lactic acid bacteria composed of Lactobacillus crispatus, Lactobacillus jensenii and Lactobacillus reuteri according to a specific ratio, and the compound lactic acid bacteria comprise Lactobacillus crispatus, Lactobacillus jensenii and Lactobacillus reuteri according to a specific ratio. According to the invention, a prebiotic compound formed by galactooligosaccharide, fructo-oligosaccharide and inulin is matched, extracts of lucid ganoderma, hericium erinaceus, poria cocos, cordyceps sinensis and inonotus obliquus are combined, and cranberry extract powder and pineapple powder are supplemented. Lactic acid bacteria form a double-layer microcapsule through electrostatic spraying, the three functional components construct a three-layer composite structure through layered pressing, and finally microcapsule powder is formed. Through targeted regulation of vaginal microecology, inhibition of inflammatory response and repair of mucosal barrier, the composition has the characteristics of stable structure, synergistic components and remarkable conditioning effect, and is suitable for health management of female genital tracts.

The invention provides a strain of Reichenach sp. H6 and application thereof, the strain has a 16S rRNA gene sequence as shown in SEQ ID NO: 1, is preserved in Guangdong Microbial Culture Collection Center, and has a preservation number of GDMCC No: 66012. The invention also provides a method for preparing the strain of Reichenach sp. H6 and application thereof. The strain is mainly used for enhancing the heat tolerance of the coral. The screened strain is clear in classification status and excellent in hereditary stability, has broad-spectrum complex polysaccharide degradation capacity, and still keeps core biological characteristics after five generations of passage. The strain has a remarkable regulation and control effect on coral co-epiphytic bacterial colony microecology, and the content of metabolites with inhibitory activity on pathogenic vibrios in coral microecology can be remarkably increased; the method is high in environmental adaptability, has a remarkable physiological status relieving effect on corals under heat stress, and can effectively improve core survival indexes of the corals under heat stress.

The invention discloses a composite material for treating inflammatory bowel diseases as well as a preparation method and application of the composite material. The composite material is formed by compounding genetic engineering probiotics for expressing catalase and surface potential modified magnetic nano-enzyme particles through electrostatic adsorption. The material has an external magnetic field assisted targeting function, the colonic colonization efficiency is remarkably improved, the retention volume is improved by 27.5 times, and the survival rate of engineering bacteria is improved by 6 times. Meanwhile, the composite material synergistically and efficiently removes ROS in the pH range of 6-7.5, the limitation that the pH adaptability of a single component is narrow is overcome, and comprehensive treatment of the inflammatory bowel disease (IBD) is achieved through a mucus-microecology-immunity-epithelium four-dimensional synergistic repair network. The preparation method is simple in operation, good in biocompatibility and high in safety, provides an innovative strategy for IBD treatment, and has important clinical application value.

The invention relates to the technical field of analysis of intestinal flora structures, and discloses a method for analyzing an adjusting effect of intestinal flora in a longevity / aging process, which comprises the following steps: step 1, selecting research objects: randomly selecting 30 old people of more than 100 years old, and selecting 30 old people of 60-70 years old as a control group; 2, basic condition investigation and physical examination of a research object; and 3, detecting technical indexes of a research object. According to the invention, the change of intestinal micro-ecology of the 100-year old people is preliminarily discussed, and the dominant flora with longevity characteristics and the biomarkers are determined. The influence of aging on intestinal microecology is obvious, so that the diversity of intestinal flora is obviously reduced, and the structural proportion of the intestinal flora is also changed; the intestinal flora spectrums of the same age group are relatively similar, that is, the core flora exists; the'core flora 'of different age groups has difference. By combining genetic backgrounds, lifestyles and environmental factors of different samples, the influence of different lifestyles and environmental factors on the structure and longevity of intestinal bacteria is determined.

The application discloses a recombinant M13 bacteriophage, a K88-targeting antibacterial preparation containing the bacteriophage and application of the antibacterial preparation, and belongs to the field of biotechnology and veterinary medicine. The antibacterial preparation contains the recombinant M13 bacteriophage rM13; a K88-targeting element is displayed on the tail fiber pIII protein of the recombinant M13 bacteriophage, and the K88-targeting element is a nanobody NBK88 with an amino acid sequence shown in SEQ ID NO:1. The engineering in the application enables the rM13 to specifically recognize, invade and lyse enterotoxigenic Escherichia coli expressing K88 fimbriae, so that the host range of the wild-type M13 is accurately guided to pathogenic bacteria. The application also provides use of the preparation in preparation of a medicine for preventing or treating ETEC infection, and a dosage form of the medicine is preferably a freeze-dried preparation or an enteric preparation capable of releasing in the intestinal tract. The preparation provided by the application has the advantages of high targeting, self-proliferation, non-damage to intestinal microecology and overcoming of bacterial drug resistance.