151 results about "Microecology" patented technology

The invention relates to the technical field of crop straw treatment and soil improvement, in particular to a rice straw returning method for improving soil fertility, which mainly comprises the following steps: crushing rice straws, soaking with a sodium hydroxide solution, and drying; then selecting specific strains, mixing and fermenting to prepare a microbial agent; preparing a composite additive in proportion; within 1-2 days after the rice is harvested, the pretreated straw, the microbial agent and the composite additive are applied to the field, deep ploughing is conducted, and irrigation is conducted; and finally, performing rotary tillage and soil preparation after straw decomposition, and monitoring and adjusting the pH value of the soil when next-stubble crops are planted. According to the method, straw is pretreated, lignin is destroyed through a sodium hydroxide solution, the particle size is accurately controlled, drying is conducted in cooperation, the microbial decomposition efficiency is improved, straw and soil are fully mixed through accurate ploughing in the field, decomposition is accelerated into nutrients, the straw can be powerfully decomposed through a microbial agent, and the soil micro-ecology is improved; the composite additive supplements various nutrients and enhances the water and fertilizer retention capability of soil.

The invention discloses a composition for regulating scalp micro-ecology and application thereof, and relates to the technical field of daily chemical products. The composition for regulating scalp micro-ecology comprises the following components: emu oil, melaleuca alternifolia leaf oil, Brazil green propolis and hydrogenated castor oil. The invention provides a composition for regulating scalp microecology and application thereof, the composition is prepared by compounding an anti-inflammatory and antibacterial component emu oil with melaleuca alternifolia leaf oil, Brazilian green propolis and hydrogenated castor oil, the composition has synergistic bacteriostasis and anti-inflammation effects, keeps scalp microbe balance, reduces red and swollen skin and itching, and also can promote the regeneration capacity of skin tissue cells.

The invention discloses a multi-stage composite constructed wetland construction method based on micro-ecological optimization, and relates to the technical field of constructed wetland ecological restoration. According to the method, fluctuation characteristics of pollutant concentration and hydraulic load and coupling risks of the fluctuation characteristics are systematically and quantitatively recognized, a high-disturbance risk area and an induction succession area are accurately divided, and a multi-stage composite constructed wetland is constructed. The micro-ecological state and succession potential of the wetland are dynamically monitored and evaluated, the wetland type layout is scientifically regulated and controlled on the basis of the hydraulic path resistance index, the non-linear impact of pollution impact on micro-ecology is effectively relieved, the ecological self-repairing capacity and toughness of the wetland system are improved, the function passivation phenomenon is avoided, and the method is suitable for large-scale popularization and application. Meanwhile, the resource allocation efficiency and the pollution treatment effect are improved, the operation and maintenance cost is reduced, and the adaptability and continuous purification capacity of the constructed wetland to the complex polluted water body are remarkably enhanced.

The invention provides a comprehensive 16S automatic analysis method and process based on Web, and from upstream sequencing data processing to downstream data analysis, the most common analysis items at present and 16S advanced analysis are included. And meanwhile, the analysis content is modularized, the data mining method and the like are diversified and deep, and different analysis modules can be selected according to individual needs. The method not only can help researchers without any biological information background to carry out 16S research on flora micro-ecology, but also can overcome the defects of some existing 16S analysis processes, can obtain an accurate and comprehensive analysis result, and meets various analysis requirements.

The invention discloses a Bacillus velez, a fermentation product filtrate thereof and a biological product thereof, belonging to the field of microbial technology. A Bacillus velez BTN-HB-F4 strain is obtained by isolating and purifying green prunes from Haba Snow Mountain in Yunnan Province. The fermentation product filtrate thereof has excellent HAS inhibition ability and significant DPPH and ABTS free radical scavenging ability, and can achieve good antioxidant effect. Secondly, at various concentrations, the fermentation product filtrate thereof can significantly reduce the proportion of SA-β-Gal-positive cells, can significantly inhibit the gene expression of matrix metalloproteinase 3, and has good anti-aging effect. In addition, the fermentation product filtrate thereof can significantly inhibit the production of zebrafish melanin, and has good whitening effect. The fermentation product filtrate can significantly inhibit the secretion of cellular inflammatory factors IL-6 and TNF-α, and has good soothing effect. Finally, the fermentation product filtrate thereof can also effectively inhibit the growth of pathogenic bacteria Staphylococcus aureus, and has the potential to regulate skin microecology.

The invention relates to the field of soil improvement, in particular to a saline-alkali soil improvement method which comprises the specific steps of deep ploughing and crushing, drainage system building and pesticide spreading, high-molecular polymer application and microbial agent inoculation. Good soil conditions are created through a physical improvement method, the soil salinity and alkalinity are rapidly reduced through a chemical improvement method, the salt-tolerant microbial agent can decompose organic matter, improve soil micro-ecology, reduce salinity toxicity and promote plant growth, and the saline-alkali soil improvement efficiency and effect are remarkably improved through mutual cooperation of the three methods; the method reduces the excessive use of chemical substances in a single chemical improvement method, realizes the ecological restoration of the saline-alkali soil through a physical method, a chemical method and a microbiological method, reduces the negative influence on the environment, improves the sustainability of improvement, effectively improves the soil structure and the ecological environment, provides good conditions for plant growth, and realizes the efficient utilization of the saline-alkali soil.

The invention discloses a recombinant M13 bacteriophage, a K88 targeting antibacterial preparation containing the bacteriophage and application of the K88 targeting antibacterial preparation, and belongs to the field of biotechnology and veterinary medicine. The antibacterial preparation comprises a recombinant M13 bacteriophage rM13; a K88 targeting element is displayed on a tail filament pIII protein of the recombinant M13 bacteriophage, and the K88 targeting element is a nano antibody NBK88 of which the amino acid sequence is as shown in SEQ ID NO: 1. Through engineering modification, the rM13 can specifically recognize, infect and split enterotoxigenic escherichia coli expressing K88 fimbriae, so that the host range of the wild type M13 is accurately guided to pathogenic bacteria. The invention also provides application of the preparation in preparation of medicines for preventing or treating ETEC infection, and the dosage form of the preparation is preferably a freeze-dried preparation or an enteric-coated preparation capable of being released in a targeted intestinal tract. The preparation provided by the invention has the remarkable advantages of strong targeting property, self-proliferation, no damage to intestinal microecology and capability of overcoming bacterial drug resistance.

The invention discloses a novel bifidobacterium infantis strain and application of extracellular products of the novel bifidobacterium infantis strain in delaying skin aging and regulating skin micro-ecology, and bifidobacterium infantis is bifidobacterium infantis BSJM24L007 which is preserved in the China General Microbiological Culture Collection Center (CGMCC for short) and has the preservation number of CGMCC No.33258. The invention further discloses a preparation method of the novel bifidobacterium infantis strain and the application of the extracellular products of the novel bifidobacterium infantis strain. The bifidobacterium infantis extracellular product prepared by the invention has an ideal effect of delaying skin aging, enables the skin to be more compact and elastic, reduces the generation of wrinkles and maintains normal metabolism of the skin, has relatively high safety, can effectively reduce the skin infection problem caused by staphylococcus aureus and propionibacterium acnes, and has a good application prospect. Skin infection and inflammation are reduced, skin health is promoted, and skin micro-ecological balance is maintained. The requirements of a modern skin external preparation on functionality and safety are met.

The invention discloses a scalp micro-ecological regulation washing and care composition containing lactobacillus metagen, and particularly relates to the technical field of daily chemical washing and care products. The washing and care composition comprises the following specific components in percentage by weight: 3%-8% of lactobacillus metagen, 1%-3% of prebiotics, 0.05%-0.2% of natural antibacterial peptide, 0.5%-1.5% of a ceramide compound, 0.3%-0.7% of a pH regulator, 0.5%-2% of a microcapsule wrapping agent, 0.2%-0.6% of a natural preservative and the balance of deionized water, wherein the total percentage is 100%. According to the invention, various components are scientifically compounded, so that the washing and care composition has an excellent effect, the lactobacillus retrogen, the natural antibacterial peptide and the natural preservative cooperate to inhibit bacteria, the diameter of an inhibition zone for common pathogenic bacteria is 14-20mm, and the prebiotics and the lactobacillus retrogen regulate scalp micro-ecology, so that the flora diversity index is increased by 30-45%; the ceramide compound and dipotassium glycyrrhizinate cooperate to reduce the TEWL value by 30%-50%, the scalp barrier is repaired, and the product is good in stability, safe, reliable and capable of comprehensively caring scalp health.

The invention belongs to the technical field of greenhouse vegetable salinization soil improvement, and particularly relates to a preparation method of a compound microbial agent for greenhouse vegetable salinization soil improvement. Comprising the following steps: S1, preparing a fermentation substrate: mixing bran, molasses, ammonium sulfate, rice hull powder and water according to a mass ratio, and sterilizing at 121 DEG C for 30 minutes for later use; s2, preparing biochar: preparing modified biochar with MgO loading capacity of 4-6% and magnesium-loaded biochar with MgO loading capacity of 5-7%; s3, preparing a compound bacterium suspension: respectively preparing bacillus amyloliquefaciens, bacillus mucilaginosus, trichoderma viride, rhizobium and saccharomyces cerevisiae into 5 * 10 < 10 > CFU / mL bacterium suspensions, and mixing the bacterium suspensions according to a volume ratio to obtain the compound bacterium suspension. Through cooperation of multiple strains and charcoal-loaded magnesium, the salinization index of the facility vegetable soil can be remarkably reduced, the soil fertility is comprehensively improved, the micro-ecology of the soil is restored, and the growth and quality of vegetables are promoted.

The invention belongs to the technical field of microorganisms, and particularly relates to combined lactobacillus salivarius and application thereof in female genital tract health. The preservation number of the strain is CCTCC NO: M20231719, and the strain has good bacteriostatic ability and dendritic cell (DC) maturation / activation stimulation ability, has no potential safety problem, can regulate vaginal micro-ecological balance, prevent infection caused by exogenous bacteria, effectively improve tumor immunosuppression microenvironment, and has good application prospects. And the method has very important significance on prevention / treatment of vaginal dysbacteriosis patients, gynecological tumors and precancerous lesions thereof.

The invention discloses an aquatic product breeding feed capable of improving flavor and a preparation method thereof, and particularly relates to the technical field of breeding feeds, the feed comprises fish meal, soybean meal, soybean protein concentrate, shrimp meal, chicken meal, wheat flour, cuttlefish paste, phospholipid, grease, mineral substances, vitamins, lettuce polyphenol, an antioxidant, a mildew preventive and the like. Wherein the lettuce polyphenol is a key functional component. The preparation method comprises the following steps: pretreating the raw materials, mixing step by step, adding grease by spraying, tempering, granulating, drying, screening, spraying the antioxidant and the like. The lettuce polyphenol regulates and controls an mTOR signal channel to promote flavor amino acid deposition and inhibit protein degradation, meanwhile, the intestinal microecology is improved, the antioxidant capacity and digestive enzyme activity are enhanced, and the content of flavor substances such as glutamic acid and glycine in aquatic products is remarkably increased. The method is suitable for culturing various aquatic products, and can effectively improve the flavor and quality of the cultured aquatic products.

The invention relates to the field of microorganism screening, in particular to a microorganism screening method capable of improving saline-alkali soil crop stress resistance and application thereof.The microorganism screening method comprises the following steps that S1, in saline-alkali soil with Na < + > larger than 0.6% and pH 8.5-10.0, suaeda glauca rhizosphere soil and root system tissue are collected and inoculated to 0.6 moL / L NaCl, and the saline-alkali soil is inoculated to a culture medium; in a liquid culture medium with the pH value of 8.0, through a gradient saline-alkaline stress enrichment-bifunctional bacterium collaborative screening technology and in combination with an embedding matrix and modified biochar compounded microbial screening agent, compared with the prior art, salt ion adsorption, flora survival rate improvement, root system nitrogen fixation and phosphate solubilizing bacterium standard-reaching rate improvement and crop biomass increase are synchronously realized, and the yield of the crops is increased. And moreover, the toxicity of salt ions is remarkably reduced, the soil micro-ecology is systematically optimized, and the method has a wide application prospect in marginal land agricultural development and saline-alkali soil ecological restoration.

The invention belongs to the technical field of bioengineering and microbiomics, and particularly relates to an acquisition method and application of a specific metabolic substrate of lactobacillus crispatus and application of a specific carbohydrate active enzyme CAZzyme gene and a polysaccharide utilization site (PUL). Genomes of lactobacillus crispatus and lactobacillus inertus are obtained from a public database, gene annotation is carried out on the genomes of the lactobacillus crispatus and the lactobacillus inertus, and a random forest model and a KEGG metabolic pathway diagram are combined to find specific CAZzyme genes, PUL and other genes with metabolic functions of the lactobacillus crispatus; a substrate which only lactobacillus crispatus can metabolize is searched based on the genome difference of the two bacteria, and the ability of the obtained substrate for promoting the growth of lactobacillus crispatus and inhibiting the growth of inert lactobacillus is evaluated through experiments. Growth and reproduction of the lactobacillus crispatus are promoted through the substrates, so that the lactobacillus crispatus accounts for a higher proportion in vagina microecology, the vagina is protected, and reproduction of other unfavorable bacteria is reduced. The assisted reproduction success rate is improved.

The present invention discloses the use of pantothenic acid in the preparation of anticaries preparations related to Streptococcus mutans, and anticaries preparations containing pantothenic acid. The invention belongs to the field of biomedicine technology. The anticaries preparations related to Streptococcus mutans include an acid production inhibitor for Streptococcus mutans, a growth inhibitor for Streptococcus mutans, an inhibitor of cariogenic toxins of Streptococcus mutans, and an inhibitor of interspecies competition of Streptococcus mutans. Pantothenic acid can be used to influence the acid production, growth, production of cariogenic toxins, and interspecies competition of Streptococcus mutans, thereby maintaining the balance of the oral microecology.

The invention discloses a composite fiber material containing Miao medicine extract and bioactive polypeptide and application of the composite fiber material in preparation of underwear, and relates to the technical field of functional textile materials. Comprising a metabolite response type core layer, a flora adaptive type sheath layer and a dual-mechanism sensing release interface arranged on the surface of the core layer, according to the invention, on the basis of a core-sheath structure and a dual-mechanism sensing interface, the composite fiber can accurately respond to skin micro-ecology, directionally release Miao medicine extract and bioactive polypeptide, protect beneficial bacteria, inhibit harmful bacteria and prevent unbalance, and can also form a component-micro-ecology-skin collaborative closed loop; micro-ecology visualization is achieved through an AI E probe, washing resistance is achieved, the function is stable, the physical performance is matched with underwear, prebiotics are supplemented in the preparation process, the health value can be enhanced, sensitive skin is matched, and the discomfort risk is reduced.

The invention relates to the technical field of cut flower postharvest preservation, and discloses a preservation pre-treatment solution for maintaining moisture-nutrition-microecological balance in a storage period of babysbreath cut flowers and a synergistic application method thereof. The pre-treatment liquid is prepared from the following components in percentage by weight / volume: 15 to 30 g / L of cane sugar, 15 to 30 g / L of trehalose, 100 to 200 mg / L of 8-hydroxyquinoline citrate, 50 to 150 mg / L of citric acid, 50 to 100 mg / L of carboxymethyl chitosan, 0.3 to 1.0 g / L of lysozyme-chitosan compound, 5 to 15 mg / L of nano silver-loaded silicon dioxide, 0.1 to 1.0 g / L of manganese citrate and 0.5 to 1.0 g / L of calcium nitrate. And 5 to 10 mL / L of glycerol and 0.5 to 5.0 mL / L of Tween-20 in percentage by volume. Through component collaborative design-process precise control-package matching innovation, the bottleneck of double loss of water and nutrition in the storage period is broken through, the pain points of no flowering and gray mold outbreak after storage are solved, a whole-process nursing scheme is provided for long-distance and short-distance transportation and storage of the babysbreath cut flowers, the commodity rate of the cut flowers is remarkably increased, and the economic benefit is increased. The method is suitable for flower harvesting bases, cold chain transportation and wholesale markets, and is especially suitable for air transportation exits.

The invention provides the food calling type river crab feed, the application effect of the food calling type river crab feed in river crab culture shows that the food calling type river crab feed has remarkable advantages in the aspects of improving the growth performance, enhancing the immune function, optimizing the intestinal microecology, improving the water quality environment and the like, the use of the fish meal is reduced through the fermented vegetable protein, and the specific composite phagostimulant has the advantages that the utilization rate of the fish meal is reduced; the palatability of the river crab feed with the fish meal content reduced is improved, the feed coefficient is reduced, the unit economic benefit is improved, the problem of environmental pollution in the breeding process is effectively relieved, and the river crab feed has good ecological and economic dual values.

The invention discloses a composition for improving immunity and delaying senescence and a preparation method thereof. Belongs to the technical field of functional health. The composition comprises the following components in parts by mass: 5-50 parts of collagen, 10-80 parts of paeonia ostii tea, 1-20 parts of an antioxidant, 3-10 parts of an intestinal microecology regulator and 1-3 parts of an energy metabolism and nerve regulator. According to the invention, through multi-system cooperation and multi-target intervention, dual effects of immune enhancement and senescence delay are realized. Through scientific compounding and a special process, the product has the advantages of stable components, uniform activity and good solubility. Animal experiments and theoretical models show that immune organs can be effectively protected, accumulation of aging markers is reduced, and a solution is provided for development of comprehensive anti-aging health products.

The invention discloses an application of a prebiotic component in enhancing athletic ability and improving athletic performance. The prebiotic component is xylooligosaccharide. Animal experiments show that mice given with xylooligosaccharide can significantly improve the abundance of probiotics and enhance the muscle strength, constant-speed endurance and other movement functions of the mice. The xylooligosaccharide serving as an efficient prebiotic can be specifically utilized by specific probiotics in intestinal tracts, the abundance of the xylooligosaccharide is remarkably improved, growth of pathogenic bacteria is inhibited, then the micro-ecological environment of the intestinal tracts is optimized, and the exercise function improvement phenomena such as increase of muscle strength and delay of fatigue in endurance exercise are shown in behavioral tests. The cross-organ integration regulation and control from the intestinal microecology to the whole body physiological state and then to the athletic performance is reflected, so that the xylooligosaccharide has important significance in enhancing the athletic ability and improving the athletic performance.

The invention provides application of beta-1, 4-glucan type cellooligosaccharide in improvement of plant rhizosphere micro-ecology and enhancement of IAA signals, and belongs to the technical field of biological agriculture. The invention provides an application of beta-1, 4-glucan type cellooligosaccharide with the polymerization degree of DP2-DP5 in improvement of plant rhizosphere micro-ecology and enhancement of IAA (Indole Acetic Acid) signals. In the invention, beta-1, 4-glucan type cellooligosaccharide can induce plant immunity in a growth allowable PTI threshold range, so that a rhizosphere microflora structure is directionally remodeled, the relative abundance of beneficial microorganisms is improved, the potential pathogen load is reduced, rhizosphere IAA and related metabolites thereof can be increased, and'disease resistance without growth inhibition 'is realized. The invention further provides an application of the beta-1, 4-glucan type cellooligosaccharide combined with rhizosphere beneficial microorganisms in biological agriculture, and growth promotion and / or nodulation nitrogen fixation effects can be enhanced.

The invention discloses a method and system for synchronously detecting host chromatin openness and in-vivo microbiome based on transposase, and belongs to the technical field of biological sequencing data analysis. According to the method, transposase is used for selectively fragmenting an open chromatin region of a host, and a microbial genome is almost randomly cut, so that synchronous enrichment of host and microbial DNA is realized; after high-throughput sequencing library construction and double-end sequencing, sequencing data is split into host source and non-host source reads through bioinformatics analysis, host chromatin state and microorganism composition are analyzed respectively, and a microorganism-host epigenetic regulation network is constructed; the invention further provides a matched DNA sequencing library and an analysis system, multi-scene research of infectious diseases, intestinal microecology, tumor microenvironment and the like is supported, a public database can be reanalyzed, and potential microbial interaction signals are mined. According to the method, the host-microorganism interaction research efficiency is remarkably improved, and a high-sensitivity and integrated technical scheme is provided for epigenetic regulation mechanism analysis.

This invention discloses a method for the synergistic metabolic analysis of multiple components of traditional Chinese medicine (TCM) integrated with the gut microbiota. After initializing the microbiota ecosystem, a solution of multi-component TCM extracts is continuously injected into a microfluidic chip. Stable isotope-labeled precursors are pulsed into a specific simulation region to perform spatial isotope encoding. A standing wave acoustic field is applied at the sampling node of the microfluidic chip, and multiphase fluid spatial splitting is performed using the difference in the primary acoustic radiation force experienced by the particles to derive cell-free TCM metabolites. The cell-free TCM metabolites are separated by chromatography and gas phase drift separation. A four-dimensional metabolic feature array is constructed, and the absolute value of the mass shift is calculated for spatial inverse decoding. Closed-loop feedback is triggered based on the rate of change of target metabolite ion abundance, increasing the amplitude of the standing wave acoustic field pressure to confine intestinal bacteria and performing in-situ enrichment of synergistic metabolic intermediates. This invention realizes the spatial metabolic trajectory tracing and network reconstruction of multiple components of TCM in a complex gut microbiota ecosystem.

The application discloses a method for improving the rhizosphere micro-ecology of tomatoes by using Pseudomonas moraviensis in combination with arbuscular mycorrhizal fungi and application thereof, wherein the strain HY-S70 belongs to Pseudomonas moraviensis and has a preservation number of CGMCC No.32673. Before planting tomatoes, arbuscular mycorrhizal fungi inoculum (preferably Glomus mosseae) is inoculated into soil at an inoculation amount of 30 spores / g inoculum; and 3-5 days after planting the tomatoes, Pseudomonas moraviensis bacterial liquid with a bacterial concentration of 1x10 9 CFU / ml is inoculated. The method provided by the application can activate phosphorus in soil, promote the growth of tomato plants, improve the rhizosphere microbial community structure of the tomato plants and optimize the rhizosphere micro-ecological environment of the tomato plants, and has a wide application prospect.

The invention discloses high-elasticity functional tofu and a preparation method thereof in the technical field of tofu processing, the high-elasticity functional tofu is prepared by adding dictyophora indusiata polysaccharide during soybean milk curdling, and the dictyophora indusiata polysaccharide has multiple biological activities such as inflammation resistance, oxidation resistance and intestinal microecology regulation, so that the health-care function of the tofu is improved; the dictyophora indusiata polysaccharide is obtained by preparing dried powder by taking fresh dictyophora indusiata as a raw material and extracting the dried powder by any one of a hot water extraction method, an ultrasonic extraction method and an enzyme extraction method. Through interaction of the bamboo fungus polysaccharide and protein in the bean curd gel, the texture quality of the bean curd is improved, and the bean curd is good in elasticity, moderate in hardness and good in chewing taste.

This invention discloses a strain of *Lactobacillus paracasei* CCFM1333 with multi-target regulatory effects and its application in regulating oral microecology, belonging to the field of microbial technology. This invention yields a strain of *Lactobacillus paracasei* CCFM1333, whose fermented milk and inactivated fermented milk not only inhibit odor-causing bacteria in the oral cavity and regulate oral microecology, but also alleviate inflammation. Specifically, it can reduce the number of odor-causing bacteria in the oral cavity of rats with increased odor-causing bacteria; downregulate the levels of serum inflammatory factors IL1β, IL-6, and IL-17A; and reduce the relative abundance of pathogenic bacteria in the oral cavity of rats, effectively regulating the oral microecology of rats.

The invention discloses targeted probiotic dietary powder for conditioning intestinal microecology and a preparation method of the targeted probiotic dietary powder. According to the invention, the micro-ecological health of intestinal tracts is obviously improved through a triple synergistic mechanism. The core probiotic combination directly acts on the key link of intestinal flora imbalance. Lactobacillus plantarum LP-12 inhibits excessive release of intestinal inflammatory factors and alleviates intestinal mucosa damage. The bifidobacterium bifidum TMC3115 enhances tight connection of intestinal epithelial cells and repairs a physical barrier; the Witzia ciliaris MKSWC01 can degrade endotoxin and block the chain reaction of chronic inflammation. Meanwhile, the fucoidin and the galactooligosaccharide are used for accurately feeding the probiotics and enhancing the colonization capability of the probiotics in a colon mucus layer, so that long-acting protection is formed. L-glutamine provides basic nutrition required by intestinal mucosa regeneration, and resistant dextrin continuously generates short-chain fatty acid to maintain a weak acid environment of colon, so that healthy flora ecology is comprehensively reconstructed.

The application discloses a brain hemorrhage prognosis improvement method based on intestinal flora regulation and relates to the technical field of intestinal microecology.The application comprises the following steps: detecting feces, starting intervention if the enterococcus abundance and neuroinflammatory metabolic markers exceed the standard, stopping the use of conventional nutrient solution, perfusing a special component containing sugar analogs, blocking the energy uptake of enterococcus, simultaneously providing a carbon source for beneficial bacteria, synchronously perfusing a microsphere suspension containing a molecular capture function, adsorbing and fixing proinflammatory metabolic waste in the intestinal tract, blocking the blood entry of the proinflammatory metabolic waste, perfusing live bacteria with different functions three times in time sequence, sequentially completing the acidification of the environment, constructing a bacterial membrane barrier and repairing the intestinal epithelium, and re-detecting after each round of intervention, selecting the maintenance period or adjusting the intensity and then recycling according to the decrease of enterococcus and markers.The application can limit the competitive advantage of enterococcus in the utilization of carbon sources after brain hemorrhage by sequentially implementing competitive inhibition of the phosphotransferase system and multi-species ecological niche occupation.

The invention discloses a strain of chicken source lactobacillus casei H0001 and application of the chicken source lactobacillus casei H0001 in improvement of intestinal microecology of chicks. The chicken source lactobacillus casei provided by the invention is clear in source, high in host adaptability and suitable for colonization in the intestinal tract of a chick in the early stage; the strain provided by the invention can significantly improve the diversity of intestinal flora and the abundance of beneficial bacteria of chicks, and improve the intestinal micro-ecological structure; according to the invention, a safe and effective micro-ecological technical means is provided for early intestinal health regulation and control of chicks to replace or reduce the use of antibiotics; and (4) the method has a good application prospect and can be used for green and healthy breeding of poultry.

The utility model discloses a sampling capsule for in-situ sampling of a digestive tract. The sampling capsule comprises a capsule shell, a sample cabin door is formed in the capsule shell, a sample cabin, a circuit board and a battery are arranged in the capsule shell, the sample cabin corresponds to the sample cabin door in position, the circuit board is electrically connected with a micro motor, the output end of the micro motor is connected with a gear set, and the gear set is used for amplifying the acting force of the micro motor. The gear set is connected with a sample cabin door on the capsule shell through a transmission shaft; the gear set comprises a driving gear connected with the output end of the micro motor, a driven duplicate gear connected with the driving gear in a meshed mode and a driven gear connected with the driven duplicate gear in a meshed mode. The device can prevent proteins, metabolites and the like from being re-absorbed by colon, can effectively reflect the structures of biome, proteome and metabolome at each stage of the digestive tract, and provides a new device for research on space-time structure of microecology of the digestive tract and composition change of the space-time structure during occurrence and development of diseases.