The present invention relates to a low protein laying hen concentrated feed with amino acid balance. The feed has the following advantages that: the animal digestive utilization rate is high, the flora balance in the digestive tract can be effectively maintained, the cost is low, the resource is saved, and the environmental pollution is reduced. The feed of the present invention is prepared by the following raw materials, wherein the raw materials comprise, by weight, 320-390 parts of soybean meal, 100-140 parts of cottonseed meal, 30-70 parts of rapeseed meal, 65.4-105.4 parts of corn pulp protein, 20-60 parts of corn protein powder, 235-275 parts of calcium carbonate, 28-36 parts of calcium hydrogen phosphate, 0.8-1.4 parts of a compound vitamin, 1.2-1.7 parts of compound trace element,13-17 parts of grease, 6.5-10.5 parts of lysine, 2-6 parts of liquid methionine, 0.8-1.2 parts of threonine, 6.5-10.5 parts of salt, 0.3-0.7 parts of an antioxidant, 1-5 parts of sodium bicarbonate, 1.2-1.7 parts of a microecology agent, 0.2-0.6 parts of phytase, 0.3-0.7 parts of a complex enzyme agent and 1-3 parts of choline choride.