17 results about "Antimikrobielle peptide" patented technology

This invention discloses a *Bacillus laterosporus* strain for the stable production of nonribosomal cyclic lipolipeptide and its construction method. The M1A gene in the Bogorol biosynthetic gene cluster is knocked out using CRISPR-Cas9 technology to block the Bogorol pathway, which competes with Brevicidine for substrates. Subsequently, the pMCCas9-S48 plasmid, which integrates S8 and S41 sgRNAs and their homologous arms as repair templates, is used to simultaneously knock out S8 and S41 family peptidase genes, preventing the expression of extracellular degradation enzymes. This invention also provides the application of the above strain in the preparation of nonribosomal cyclic lipolipeptide of Brevicidine and a method for preparing the nonribosomal cyclic lipolipeptide. This strain, through reducing metabolic competition and preventing product degradation, exhibits rapid cell growth, stable production of Brevicidine, and significantly reduced degradation rate, resulting in a 66.13% increase in fermentation yield compared to the unmodified strain. This invention solves the problems of degradation and unstable yield in the production of Brevicidine, provides an effective technical approach for the industrial production of Brevicidine, and provides important technical support for the development and application of novel antimicrobial peptide drugs.

This invention relates to Bacillus vesiculosus antimicrobial peptides and their application in food preservation, belonging to the field of biotechnology. The amino acid sequence of the antimicrobial peptide is shown in SEQ ID NO: 1. The antimicrobial peptide provided by this invention is a novel antimicrobial peptide in structure, enriching the antimicrobial peptide database; this antimicrobial peptide can effectively inhibit the normal growth of Fusarium oxysporum, has a broad antimicrobial spectrum, and has potential development and application prospects; this antimicrobial peptide can effectively extend the refrigerated shelf life of dragon fruit, and has good application prospects in fruit preservation.

This invention relates to the field of crop disease control technology, specifically to a composition containing antimicrobial peptides that enhances crop disease resistance. The composition comprises a synergistic and stable antimicrobial peptide carrier, a toad antimicrobial peptide (Buforin), cephalosporin B, and an antimicrobial peptide activator loaded onto the synergistic and stable antimicrobial peptide carrier. This invention prepares the synergistic and stable antimicrobial peptide carrier by reacting a mixture of cystamine, kasugamycin, and sorbitol glycidyl ether. This carrier enables precise controlled release of antimicrobial peptides at target sites, significantly prolongs the half-life of antimicrobial peptides in plants, solves the problems of easy degradation and short duration of action of conventional antimicrobial peptides, and synergistically enhances the stability of antimicrobial peptides, preventing aggregation or inactivation during loading and storage, thereby improving the bioavailability and persistence of antimicrobial peptides in field application.

Antimicrobial peptides of general formula XX1X2C X3X4X5CXXX&XgCYX10X11CX12X13 are provided. Also provided are certain formulations containing these peptides and methods of using these peptides for treating skin infections in an animal in need thereof. 20 24 21 96 45 12 S ep 2 02 4 S e p 2 0 2 4 A N T I M I C R O B I A L P E P T I D E S A N D M E T H O D S O F U S I N G S A M E A B S T R A C T 2 0 2 4 2 1 9 6 4 5 1 2

This invention discloses a method for efficiently extracting and enriching sludge-based antimicrobial peptides from activated sludge, belonging to the field of activated sludge resource recycling and utilization. The invention removes impurities from the activated sludge by releasing intracellular and extracellular proteins through low-temperature hot alkaline hydrolysis, followed by enzymatic hydrolysis with proteases to obtain a crude extract containing peptides of different molecular weights. Further purification involves primary purification to remove impurities and secondary purification to separate amino acids and peptides from the organic matter mixture, yielding purified antimicrobial peptide products of different molecular weights. Antimicrobial activity is assessed using an inhibition zone assay, resulting in a highly effective antimicrobial sludge-based antimicrobial peptide molecular template. This template is used to prepare a magnetic molecularly imprinted polymer (Fe3O4@MIPs). Fe3O4@MIPs are then used to adsorb and enrich the highly effective antimicrobial sludge-based antimicrobial peptides in the crude extract. After elution with an eluent, highly effective antimicrobial sludge-based antimicrobial peptides are obtained.

This invention discloses an antimicrobial peptide LR-24 and its application in the preparation of antimicrobial drugs, belonging to the field of biomedical technology. The amino acid sequence of the antimicrobial peptide LR-24 is shown in SEQ ID NO.2. The antimicrobial peptide LR-24 provided by this invention has a highly efficient killing ability against a variety of Gram-negative / positive bacteria and exhibits broad-spectrum stability. It maintains strong antimicrobial activity under conditions of salt ions, 50% serum, proteases, and high temperatures, improving the problem that the activity of antimicrobial peptides decreases in some complex environments. Furthermore, while maintaining antimicrobial activity, the antimicrobial peptide LR-24 shows significantly reduced hemolytic activity and cytotoxicity, making it a promising candidate for novel antimicrobial drugs with great clinical application potential.

This invention, based on deep learning and molecular dynamics simulations, yielded a novel antimicrobial peptide, yuce 4, designed using artificial intelligence, exhibiting highly efficient and broad-spectrum antimicrobial activity. The existence of the α-helix in yuce 4 was confirmed by nuclear magnetic resonance (NMR), circular dichroism (CD) chromatography, and Alphafold 2 three-dimensional structure prediction. Furthermore, mutants of yuce 4 were designed, showing a 4-8 fold increase in activity against Stenotrophomonas maltophilia WH006 and Pseudomonas aeruginosa PAO1, with several analogs achieving a MIC of 8 μg / mL. This invention also demonstrates the potential of deep learning and molecular dynamics simulations in accelerating the discovery of antimicrobial peptides and shows promise as an important tool for developing novel antimicrobial peptides.

This invention belongs to the fields of biomedicine, beauty, and personal care health technology, and discloses an oil-controlling, acne-reducing, and antimicrobial peptide co-amorphous assembly, its preparation method, and applications. The method includes: mixing an octyl glycine solution with an antimicrobial peptide solution, rotary evaporation, and drying to obtain an antimicrobial peptide co-amorphous compound; mixing a cyclodextrin compound with water, adding the antimicrobial peptide co-amorphous compound, heating the reaction, and spray drying to obtain the oil-controlling, acne-reducing, and antimicrobial peptide co-amorphous assembly. The oil-controlling, acne-reducing, and antimicrobial peptide co-amorphous assembly provided by this invention efficiently solubilizes octyl glycine through co-amorphous technology and nano-assembly, improving its solubility and bioavailability; simultaneously optimizing the transdermal absorption of the antimicrobial peptide and achieving targeted accumulation in the dermis; the broad-spectrum antibacterial and oil-controlling effects of octyl glycine can be organically combined with the targeted antibacterial effects of the antimicrobial peptide, providing a new strategy for oily and acne-prone skin care; cyclodextrin inclusion enhances stability; the process is mild, simple, environmentally friendly, and easy to industrialize.

Modified synthetic peptides exhibiting improved antimicrobial activity in comparison to wild-type peptide sequences as well as compositions and plant parts which have been treated with the peptides are provided. Methods of using the modified peptides to control microbial infections of plant and animal subjects are also provided.

This invention discloses an antimicrobial 10-peptide, characterized by the sequence: w-k-r-w-r-r-w-w-r-r(dTrp-dLys-dArg-dTrp-dArg-dArg-dTrp-dTrp-dArg-dArg), where all amino acids are D-type amino acids. The antimicrobial peptide provided by this invention exhibits good antimicrobial activity against a variety of pathogenic bacteria. This antimicrobial peptide can be synthesized using the Fmoc solid-phase chemical method, which is easy to synthesize and exhibits good in vivo antimicrobial activity. This antimicrobial peptide exhibits broad-spectrum killing activity against multidrug-resistant Acinetobacter baumannii, carbapenem-resistant Pseudomonas aeruginosa, as well as standard strains of Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Enterobacter cloacae, methicillin-resistant Staphylococcus aureus, and Staphylococcus aureus. It has low hemolytic toxicity, and at low to medium concentrations (2 μg / mL to 32 μg / mL), the cell survival rate can reach more than 50%. It has no toxic effect on cells, good stability, strong operability, and low cost.

This invention discloses a wound repair gel containing *Pseudolarix amabilis* oil, its preparation method, and its application, belonging to the field of gel technology. The wound repair gel comprises *Pseudolarix amabilis* oil, hyaluronic acid, chitosan, antimicrobial peptides, and a gel matrix, wherein the gel matrix is ​​carbomer and xanthan gum. The preparation method includes first swelling and neutralizing carbomer and xanthan gum to form a gel matrix, then adding *Pseudolarix amabilis* oil, hyaluronic acid, chitosan, and antimicrobial peptides and mixing them at low temperature to form a sol mixture. After homogenization, degassing, and heating, a semi-solid gel is formed, and finally sterilization is performed to obtain the final product. This invention's gel combines *Pseudolarix amabilis* oil with hyaluronic acid, chitosan, and antimicrobial peptides, exerting anti-inflammatory, antibacterial, angiogenesis-promoting, and epithelial cell migration-promoting effects. It can significantly accelerate wound healing, inhibit scar hyperplasia, and exhibits excellent biocompatibility and no cytotoxicity.

This invention belongs to the field of aquaculture technology and discloses a method for preparing a compound metabiotic to improve the stress resistance of aquatic animals and its application in feed. The invention discloses a method for preparing a compound metabiotic that employs a spatiotemporal sequential fermentation strategy of "first propagation, then induction, and finally compensation": First, Bacillus subtilis aerobic fermentation degrades the substrate to produce specific signal peptides; then, Lactobacillus plantarum and Saccharomyces cerevisiae are introduced, utilizing the previous metabolites to induce Lactobacillus plantarum to efficiently synthesize antimicrobial peptides and organic acids, and combined with the metabolic compensation effect of Saccharomyces cerevisiae, significantly enhancing the functional component gradient of the metabiotic. The compound metabiotic prepared by this invention has high concentrations of organic acids, active enzyme systems, and high release rates of β-glucan, which can significantly improve the stress resistance and survival rate of aquatic animals such as shrimp under extreme environments such as low salinity, high ammonia nitrogen, and low oxygen, solving the industry pain point of probiotic activity loss during high-temperature feed pelleting.

This invention belongs to the field of biotechnology, specifically relating to an antimicrobial peptide for improving intestinal health and combating diarrhea in animals, and its preparation method. The invention prepares a novel antimicrobial peptide with the amino acid sequence shown in SEQ ID NO:1. Experiments have verified that after one week of feeding in an unfamiliar environment, the antimicrobial peptide can slightly increase the levels of propionic acid and butyric acid in the experimental group of cats. Furthermore, the overall BCFAs levels in the experimental group were lower than those in the control group before and after transportation. Although there were no significant differences in α-diversity and β-diversity between the experimental group fed the antimicrobial peptide and the control group, feeding the antimicrobial peptide significantly increased the levels of Bacteroides, Parabacterium, and Prevotella in the experimental group, and significantly reduced the levels of harmful bacteria Salmonella and Actinomyces. Therefore, the antimicrobial peptide of this invention can be used to prepare products for improving intestinal health and combating diarrhea in animals, such as feed additives or oral liquids.

This invention relates to the field of biomedical technology, providing a cascade-responsive, time-controlled release three-layer microsphere. The microspheres are prepared via a W / O / W emulsion method, with a PLGA core simultaneously encapsulating the chemotherapy drug paclitaxel, the traditional Chinese medicine Curcuma zedoaria oil, and an MMP-9 responsive peptide. An outer aqueous phase of sodium alginate provides negatively charged groups. Then, through pH-responsive chitosan electrostatic adsorption, freeze-dried Lactobacillus curvature is directionally anchored to the surface to form a probiotic shell. The microspheres exhibit a three-stage time-series release characteristic: within 0-2 hours, the probiotic shell preferentially colonizes, reducing serum E2 levels and secreting antimicrobial peptides. Within 2-6 hours, the acidic microenvironment regulated by the probiotics triggers a chitosan layer response, releasing Curcuma zedoaria oil to induce ferroptosis in cancer cells and reverse the immunosuppressive microenvironment. Within 6-72 hours, the tumor microenvironment overexpresses enzymatically digested peptides, releasing the core paclitaxel for long-term synergistic killing of tumor cells. This microsphere, through a triple mechanism of "microbial community reconstruction - immune reversal - tumor clearance," synergistically clears tumors and restores microenvironmental homeostasis.

The invention discloses an antibacterial peptide PXJ11 and application of the antibacterial peptide PXJ11. According to the present invention, an amino acid sequence represented by SEQ ID NO.1 is subjected to acetyl end-capping and amino end-capping modification to obtain the antibacterial peptide having inhibition effects on a variety of pathogenic bacteria, and the antibacterial peptide is named as antibacterial peptide PXJ11; the antibacterial peptide PXJ11 can inhibit pathogenic bacteria such as escherichia coli, staphylococcus aureus and xanthomonas citri, can also inhibit pathogenic fungi such as magnaporthe oryzae, and can be used for preventing and treating diseases / diseases caused by the pathogenic bacteria. The invention provides a novel biological control preparation for preventing and treating pathogenic bacteria such as xanthomonas citri and the like and diseases caused by the pathogenic bacteria, and the preparation is green and safe.

This invention belongs to the field of bioengineering technology and relates to marine-derived antimicrobial peptide FP2667 and its use in preparing skin antimicrobial drugs. The amino acid sequence of the antimicrobial peptide FP2667 is shown in SEQ ID NO.1. This antimicrobial peptide FP2667 is derived from marine Streptomyces. Streptomyces sp This invention also provides the DNA molecule encoding the antimicrobial peptide, the recombinant expression vector, and the engineered strain, achieving efficient heterologous expression of FP2667 in vitro. Experimental verification shows that the antimicrobial peptide exhibits good stability under the tested conditions, has a stable spatial structure and possesses environmental adaptability unique to marine microorganisms, while also demonstrating highly efficient nucleic acid (DNA / RNA) binding activity and broad-spectrum antimicrobial activity, showing significant industrial application prospects in the fields of medicine, food, and environmental remediation.