101 results about "Antimikrobielle peptide" patented technology

The invention discloses a technique for preparing antimicrobial peptide by using Bacillus subtilis, belonging to the technical field of bioengineering. By using Bacillus subtilis BRT39 capable of generating broad-spectrum high-efficiency antimicrobial peptide as a production strain, liquid seed preparation, fermentation liquid preparation, fermentation liquid pretreatment, antimicrobial peptide extraction and refinement, antimicrobial peptide dry powder preparation and other techniques are carried out to prepare the high-purity antimicrobial peptide finished product, wherein the activity recovery rate is up to higher than 43.5%. The invention has the advantages of simple technique, high yield of antimicrobial peptide, and high purity; the prepared antimicrobial peptide dry powder belongs to a natural antimicrobial agent and has no toxic or side effect, and thus, can be used as a food preservative, feed additive, agricultural biological control agent or animal disease preventing agent; and the antimicrobial peptide has the advantages of no toxicity, no residue, no drug resistance and high safety.

The present invention relates to DNA constructs that can produce antimicrobial materials efficiently from microorganisms and the preparation method thereof. The present invention also relates to the useful vector for the DNA construct. The DNA construct according to the present invention comprises a first gene coding for entire, a part of or a derivative of purF gene and a second gene coding for antimicrobial peptide. According to the present invention, antimicrobial peptides can be mass-produced by the following steps: preparing an expression vector containing a DNA construct comprising a first gene coding for an entire, a part of or a derivative of purF gene and a second gene coding for antimicrobial peptide; transforming the bacterial host cells with the above-mentioned vector, culturing the transformed cell to express the above-mentioned DNA construct; and recovering the above antimicrobial peptide.

The invention discloses albumen powder richening in antimicrobial peptide fly maggot, a preparation method for the albumen powder and a feed additive of the albumen powder. The albumen powder richening in the antimicrobial peptide fly maggot is prepared by a method comprising the following steps of: (1) feeding flies, luring the flies to lay eggs and feeding larva by using feed after incubation; (2) conducting ultrasound induction on the larva; (3) continuously feeding, collecting the larva, washing, conducting disinfection treatment, and conducting tissue homogenate smashing; and (4) filtering, treating filtrate at 0-5 DEG C, removing surface lipid compositions, drying to obtain the albumen powder; adding auxiliary materials in the albumen powder, pelletizing to obtain the feed additive. A ultrasound stimulation mode under specified conditions is adopted to induce the larva to enable an antimicrobial peptide active ingredient content in a body of a housefly to obviously improve, and the feed additive has certain growth promotion and disease-resistant strengthening effects on litopenaeus vannamei, crucian and the like.

The invention belongs to the technical field of processing methods for obtaining chitin, antimicrobial peptides and insect protein powder from insects and particularly relates to a comprehensive treatment method and an application of insects The treatment method comprises the steps of smashing insects, carrying out supercritical extraction, fully extracting and separating to obtain chitin and antimicrobial peptides, formulating the chitin and antimicrobial peptides to obtain a feed additive for live stock as well as fat, wherein the fat is used as high-voltage transformer oil, the residue after extraction is insect protein powder which can be used as a protein material of feed for the live stock. According to the treatment method, the insects are treated to produce products with high added values, all effective components are fully used, and the resource is effectively saved.

The invention belongs to the technical field of insect feed additives, and particularly discloses an insect additive for poultry and livestock feeds and application of the insect additive. The additive comprises the following components in part by weight: 20 to 50 parts of insect albumen powder, and 1 part of chitin and antimicrobial peptide. The chitin and the antimicrobial peptide which are extracted from insects are used for compounding the additive for the feeds, and the quality of the feed is improved, so that the quality of raised animals is effectively improved, and safe and healthy meat and fish or egg products are produced.

The invention discloses a Saddletail grouper antimicrobial peptide LEAP-2 gene, vector, recombinant strain and protein, and application thereof. The amino acid sequence of the Saddletail grouper antimicrobial peptide LEAP-2 precursor protein is disclosed as SEQ ID NO.2, or the Saddletail grouper antimicrobial peptide LEAP-2 precursor protein is a protein with identical or higher activity prepared by performing substitution, deletion and/or addition on one or more of amino acids and/or terminal modification on the amino acid sequence disclosed as SEQ ID NO.2. The invention also discloses a Saddletail grouper antimicrobial peptide LEAP-2 gene sequence and a LEAP-2 mature peptide gene mLEAP-2 nucleotide sequence subjected to code modification; and thus, the Saddletail grouper antimicrobial peptide LEAP-2 gene enriches the gene bank of grouper, can be used for preparing a recombinant protein, antimicrobial preparation, fish immune preparation or feed additive, and provides new theoretical and practical basis for physiologic immunity research of grouper.

The invention belongs to the field of gene engineering in the biotechnology pharmaceuticals industry, and particularly relates to pig antimicrobial peptide NK-lysin fusion protein and an encoding gene and application thereof. The amino acid sequence of the pig antimicrobial peptide NK-lysin fusion protein is shown in SEQIDNO: 2 in the specification. The advantages of antimicrobial peptide and the advantages of Pichiapastoris are utilized in the pig antimicrobial peptide NK-lysin fusion protein very well, the encoding gene of the pig antimicrobial peptide NK-lysin fusion protein can be efficiently shown in the Pichiapastoris, and the production cost of the antimicrobial peptide is reduced. The pig antimicrobial peptide NK-lysin fusion protein is applied to preparing medicines for inhabiting tumor cells, is a superior anti-cancer drug and has very high economic value and huge social benefits.

The invention discloses construction of gene engineering bacteria for expressing recombinant cationic antimicrobial peptides (AMPs) G13 escherichia coli, and belongs to the technical field of gene engineering and biology. A fusion head gene is connected with an AMPs gene to construct recombinant plasmid and convert escherichia coli BL21 competent cells, so that the expression yield is improved, and more effective treatment is obtained. The invention aims to design a shorter fusion head which has higher hydrophobic property, and the C final fragment of the fusion head has more negative charges, so that the fusion head can protect small molecular target AMPs; the toxicity of the AMPs to host cells is neutralized; and the cationic AMPs comprise alpha spiral amphipathic AMPs with net positivecharges and contain nucleotide sequences SEQ ID: NO 1 and SEQ ID: NO 2. According to the construction method, DNA recombination and gene engineering technology such as restriction endonuclease cutting, ligase connection and the like are used.

The invention belongs to the technical field of stem cell treatment, and mainly relates to construction of mesenchymal stem cells modified by antibacterial fusion protein, and application of the mesenchymal stem cells in sepsis. Aiming at the problems that the mesenchymal stem cells are weaker in antimicrobial peptide LL-37 secretion function and cannot easily achieve good antibacterial, anti-endotoxin and anti-inflammatory effects, a recombinant carrier is constructed, a human LL-37 antimicrobial peptide gene and a human bactericidal/permeability enhanced protein active fragment gene rBPI21 are connected in series and are cloned into a virus vector, and the mesenchymal stem cells are infected with a virus and are modified by the virus, so that the mesenchymal stem cells can efficiently secrete and express the broad-spectrum antimicrobial fusion protein and further obtain high efficiency broad-spectrum antibacterial ability while having the ability of regenerating and repairing tissues. The stem cell treatment is effectively combined with gene therapy, so that a new choice is provided for the treatment of the sepsis.

Disclosed is preparation of a cocktail as a therapeutic agent for cow mastitis and use thereof. A compatibility of phages against the main pathogenic bacteria of cow mastitis is utilized in combination with a specific preparation method to prepare and obtain a therapeutic cocktail preparation. The therapeutic phage cocktail preparation for cow mastitis provided in the disclosure is easy to prepare, short in course of treatment and quick in effect. It has better therapeutic effect on both clinical cow mastitis and recessive mastitis than that of antibiotics and antimicrobial peptide and is expected to solve the problem of bacterial resistance in the treatment of cow mastitis, thereby reducing or getting rid of the troubles of diseases such as mastitis in cows, which is of potential development value in cow farming.

The invention relates to an ancient defense polymer having biological activity. The polymer comprises both discrete hydrophilic blocks containing cationic charge(s) and discrete hydrophobic blocks, making it amphipathic and antimicrobial in a manner analogous to natural antimicrobial peptides that form an ancient defense mechanism employed by many organisms. Such a polymer may be formed into devices designed to resist infection or bound to the surface of an apparatus to lend the biological activity to the surface of the apparatus.

The invention provides a safe and efficient animal semen disinfector and a preparation method therefor, which are aimed to fill the blank of animal semen disinfectors in the market. The safe and efficient animal semen disinfector includes the following components in percentage by weight: 4.0 to 6.0 percent of povidone iodine, 0.5 to 1.0 percent of antimicrobial peptides, 0.2 to 0.3 percent of vitamins, 0.1 to 0.2 percent of ethylenediamine tetraacetic acid, and distilled water. A mixture of the povidone iodine and the antimicrobial peptides is used as the semen disinfector for semen purification and disinfection for economic animals. Tests prove that the animal semen disinfector can safely kill the five commonest and most harmful viruses in boar semen, i.e., classical swine fever virus (CSFV), porcine circovirus type 2 (PCV-2), pseudorabies virus (PRV), porcine reproductive and respiratory syndrome virus (PRRSV), and porcine parvovirus (PPV), as well as other most pathogenic microorganisms; meanwhile, the animal semen disinfector can prolong the sperm motility and the sperm preservation time to a certain extent, and is non-toxic and harmless to fertilized ovums and embryos.

The invention relates to an acne-preventing and acne-removing composition containing antimicrobial peptide and application thereof, which belong to the field of biotechology. The acne-preventing and acne-removing composition containing antimicrobial peptide mainly is prepared from the following components in percentage by weight: 0.01 to 50 percent of antimicrobial peptide, 0.01 to 50 percent of propolis extract, 1 to 99 percent of butanediol and 1 to 99 percent of deionized water. Aimed at the formation mechanism of acne, the acne-preventing and acne-removing composition containing antimicrobial peptide which is disclosed by the invention is scientifically formulated, all the bioactive substances are synergistic, the acne-preventing and acne-removing composition containing antimicrobial peptide has the effects of regulating skin grease secretion, restoring skin pH, resisting oxidation, resisting and killing microbes, diminishing inflammation and relieving itching, enhancing skin immunity, promoting wound healing and the like, and from multiple targets and multiple aspects, the acne-preventing and acne-removing composition containing antimicrobial peptide has a remarkable effect inpreventing and removing acne, and is safe and nonirritating for skin.

This invention relates to characterisation of the biosynthetic gene cluster for the lantibiotic actagardine, identification of a novel variant of actagardine and its biosynthetic cluster, and methods of production and use of actagardine, a novel actagardine variant, herein referred to as actagardine B, and variants of both of these produced according to this invention, utilizing genes from the characterised biosynthetic gene clusters.

The invention relates to a wild type Hydramacin-1 antimicrobial peptide mutant and a preparation method of the Hydramacin-1 antimicrobial peptide mutant. By searching for natural antimicrobial peptide Hydramacin-1 active center, on the basis of determining the active center, replacing the eighth amino acid threonine with aspartic acid or other amino acid, the result shows that the mutant T8D has more excellent antimicrobial property. The invention also discloses a method for expressing the mutant protein in yeast. The method is simple, and the expressed protein has higher antimicrobial activity, and can be applied in the fields such as bio-pharmaceuticals, breeding industry, food industry and feed industry, thereby having wide application prospect and economic significance.