131 results about "Antimikrobielle peptide" patented technology

The invention relates to the technical field of computer-aided drug research and development, and provides an antibacterial peptide function recognition optimization method and system based on deep learning and LLM. Systematic innovation is performed in multiple links such as feature extraction, data generation and reasoning verification by introducing a fusion mechanism of a large language model and a deep learning model; and the accuracy and robustness of antibacterial peptide function prediction are obviously improved. On one hand, a strict feature extraction process and a structured cue word template are constructed, sequence information and physicochemical property indexes of the antibacterial peptide and a prediction result of a deep learning model can be effectively integrated, and the prediction result is reasonably corrected and optimized through the reasoning ability and knowledge verification mechanism of a large language model. The misjudgment risk of a traditional deep learning method under the condition of insufficient samples or incomplete features is effectively reduced, and the prediction accuracy and stability are remarkably improved.

A query-based generic end-to-end molecular optimization (“QMO”) system framework, method and computer program product for optimizing molecules, such as for accelerating drug discovery. The QMO framework decouples representation learning and guided search and applies to any plug-in encoder-decoder with continuous latent representations. QMO framework directly incorporates evaluations based on chemical modeling, analysis packages, and pre-trained machine-learned prediction models for efficient molecule optimization using a query-based guided search method based on zeroth order optimization. The QMO features efficient guided search with molecular property evaluations and constraints obtained using the predictive models and chemical modeling and analysis packages. QMO tasks include optimizing drug-likeness and penalized log P scores with similarity constraints and improving the target binding affinity of existing drugs to pathogens such as the SARS-CoV-2 main protease protein while preserving the desired drug properties. QMO tasks further improves optimizing antimicrobial peptides toward lower toxicity.

The invention discloses an antibacterial peptide generation method, system, equipment and medium, and relates to the technical field of antibacterial peptide generation, the method comprises the following steps: obtaining protein sequence data; inputting the protein sequence data into an antibacterial peptide generation model for training, and performing autoregression sampling on the protein sequence data through an LSTM module to generate an amino acid sequence; inputting the amino acid sequence into a Transform module, performing word segmentation according to characters, converting the amino acid sequence after word segmentation into an id sequence, and mapping the id sequence into an embedded vector; encoding the embedded vector through an encoder, and inputting global features extracted by encoding into a decoder to generate a new amino acid sequence; identifying and screening the antibacterial characteristics of the new amino acid sequence to obtain an antibacterial peptide sequence; inputting to-be-generated protein sequence data into the trained antibacterial peptide generation model to generate an antibacterial peptide sequence; the method improves the novelty and antibacterial property of the new polypeptide sequence.

The invention belongs to the technical field of polypeptide drugs in biochemistry, and discloses three antibacterial peptide analogues as well as a preparation method and application thereof, the sequence of an antibacterial peptide P-1 is CH3CO-GGGENIKKILSKIKLLK-NH2, the sequence of an antibacterial peptide P-2 is CH3CH2CH2CO-GGGENIKKILSKIKLLK-NH2, the sequence of an antibacterial peptide P-3 is CH3 (CH2) 6CO-GGGENIKKILSKIKLLK-NH2, the carboxyl ends of polypeptides are subjected to amidation, and the polypeptides are all positively charged when the pH is equal to 7. The artificially synthesized antibacterial peptide disclosed by the invention shows a remarkable sterilization effect on multidrug resistant staphylococcus epidermidis. P-1, P-2 and P-3 have small molecular weight, are convenient to synthesize, have broad-spectrum bactericidal activity, are self-assembled into nanoparticles, have low cytotoxicity, resist trypsin degradation and the like, and have wide application prospects.

Presented herein are systems and methods for using machine learning (ML) models to identify species-biased antimicrobial peptides (SB-AMPs) that target select microbial population. A computing system may retrieve a training dataset comprising AMP sequences and non-AMP sequences. The AMP sequences may target the select microbial populations. The computing system may generate, for each of the AMP and non-AMP sequences, a respective plurality of properties. The computing system may provide as input to a ML model, the AMP sequences, the non-AMP sequences, and the respective plurality of properties for each of the AMP sequences and non-AMP sequences. The computing system may determine, based on providing the input to the ML model, a set of rules defining values for the respective plurality of properties to discriminate between the AMP sequences and the non-AMP sequences.

The invention relates to a preparation method of Parasin I protein or Parasin I fermentation liquor. The preparation method comprises the following steps: obtaining a yeast strain capable of secreting the Parasin I protein; inoculating the saccharomycetes strain into a specific liquid culture medium; fermenting the yeast strain and obtaining Parasin I fermentation liquor; the fermentation liquor comprises Parasin I protein; wherein the pH value of the specific culture medium is 6-9, the inoculum size of the saccharomycetes strain in the specific culture medium is 0.005-0.015 OD / mL, and the temperature of the fermentation saccharomycetes is 28-33 DEG C. The antibacterial peptide Parasin I protein or Parasin I fermentation liquor is simple in preparation method and high in yield, the immunity of fishes can be remarkably improved, and the survival rate of the fishes infected by pathogens is increased.

The invention discloses a dual-network injectable hydrogel as well as a preparation method and application thereof. The hydrogel is methacrylated gelatin / oxidized hyaluronic acid loaded with metal polyphenol nano-particles, and the metal polyphenol nano-particles are copper-gallic acid nano-particles which contain quaternized chitosan coatings and are loaded with antibacterial peptide HHC36. After the hydrogel is injected into a periodontal pocket, the hydrogel can adapt to different defect shapes, and the CuGA-coated HHC36-coated QCS nanoparticles are released by responding to a periodontitis microenvironment, so that the effects of resisting periodontal pathogenic bacteria, removing local excessive ROS (reactive oxygen species), effectively promoting fibroblast proliferation, collagen deposition, extracellular matrix accumulation and angiogenesis and further promoting periodontal bone regeneration are achieved. The preparation process is simple, the injectable hydrogel is low in price and easy to obtain, the injectable hydrogel can be prepared through simple steps, and a new thought is provided for treatment of periodontitis.

The invention discloses a scalp micro-ecological regulation washing and care composition containing lactobacillus metagen, and particularly relates to the technical field of daily chemical washing and care products. The washing and care composition comprises the following specific components in percentage by weight: 3%-8% of lactobacillus metagen, 1%-3% of prebiotics, 0.05%-0.2% of natural antibacterial peptide, 0.5%-1.5% of a ceramide compound, 0.3%-0.7% of a pH regulator, 0.5%-2% of a microcapsule wrapping agent, 0.2%-0.6% of a natural preservative and the balance of deionized water, wherein the total percentage is 100%. According to the invention, various components are scientifically compounded, so that the washing and care composition has an excellent effect, the lactobacillus retrogen, the natural antibacterial peptide and the natural preservative cooperate to inhibit bacteria, the diameter of an inhibition zone for common pathogenic bacteria is 14-20mm, and the prebiotics and the lactobacillus retrogen regulate scalp micro-ecology, so that the flora diversity index is increased by 30-45%; the ceramide compound and dipotassium glycyrrhizinate cooperate to reduce the TEWL value by 30%-50%, the scalp barrier is repaired, and the product is good in stability, safe, reliable and capable of comprehensively caring scalp health.

The invention discloses a preparation method of a recombinant lactic acid bacteria preparation for expressing antibacterial peptide and interleukin 22 (IL-22) and application of a composite microecological preparation composed of the antibacterial peptide and the interleukin 22 in prevention and treatment of animal bacterial infection diseases. The probiotic preparation provided by the invention comprises lactic acid bacteria, antibacterial peptide and interleukin, the recombinant lactic acid bacteria preparation can be taken orally without generating toxicity to an organism, and the recombinant lactic acid bacteria preparation can be colonized in intestinal tracts and secrete the antibacterial peptide and IL-22. The antibacterial peptide secreted by the recombinant lactic acid bacteria can punch bacterial cell membranes to cause bacterial death, does not cause generation of drug-resistant bacteria, and can reduce or replace the use of antibiotics; iL-22 secreted by the recombinant lactic acid bacteria can promote the repair of damaged tissues of intestinal tracts, activate the immune system of the organism and enhance the resistance; the probiotic effect of lactic acid bacteria can regulate the balance of intestinal flora of the organism, promote nutrient absorption and enhance the immunity of the organism. The preparation provided by the invention has the advantages of innovativeness, good safety, no toxic or side effect, simple administration mode, remarkable effect and the like, can effectively reduce the use of antibiotics, and provides a new solution for preventing and treating bacterial diseases in animal husbandry.

The invention provides a bovine colostrum probiotic compound preparation as well as a preparation method and application thereof. The compound preparation provided by the invention comprises the bovine colostrum powder subjected to activity protection and the compound probiotics, and the bovine colostrum powder subjected to activity protection is obtained by adopting a microcapsule double-layer embedding and freeze-drying technology, so that the gastric acid retention rate (2h) of immune globulin in the bovine colostrum powder is greater than or equal to 90%, and IgG contained in the bovine colostrum powder is ensured to be efficiently delivered to intestinal tracts to play an immune regulation role. Wherein the compound probiotics comprise four stages of functional echeles formed by more than or equal to 6 strains, and the four stages of functional echeles comprise colon colonization major flora, gastric acid-resistant pioneer flora, antibacterial peptide secretion auxiliary flora and inflammation regulation backup flora. Through cooperation of the components in the compound preparation, the compound preparation has excellent immune enhancement and metabolic regulation functions.

The invention provides periorbital secretion inhibition gel containing natural plant polyphenols and a preparation method thereof, and relates to the field of pet eye care, the gel is chelated with iron ions through EGCG to block a porphyrin oxidation pathway, cooperates with zinc ions to inhibit bacterial growth and plays a role in multiple mechanisms of targeted removal of bacterial biofilms through antibacterial peptide LL-37; in-vitro tests prove that the functional components in the gel have a remarkable synergistic effect, proliferation of staphylococcus aureus can be effectively inhibited, deposition of porphyrin is remarkably inhibited, and formation of tear stains is relieved; animal experiment results show that the gel has a remarkable tear stain removal effect and good periorbital safety, after two weeks of treatment, the periorbital tear stain area is reduced by 75.97% compared with that of a bear dog, the eye McDonald-Shiadduck stimulation score is also remarkably reduced, corneal fluorescein staining detection results of all tested animals are negative, and the gel has a good clinical application prospect. A mild and effective new thought is provided for inhibiting the periocular secretions of the pets.

The invention belongs to the technical field of biological medicine and nanotechnology, and particularly relates to nanoparticles loaded with curcumin and antibacterial peptide as well as a preparation method and application of the nanoparticles. The nanoparticles loaded with the curcumin and the antibacterial peptide are prepared by the following steps: taking PEI-PLGA and the curcumin as raw materials, and preparing PEI-PLGA-Cur nanoparticles; pEI-PLGA-Cur nanoparticles and the antibacterial peptide are used as raw materials, and the nanoparticles loaded with the curcumin and the antibacterial peptide are prepared. The nanoparticles loaded with the curcumin and the antibacterial peptide provided by the invention have a photodynamic therapy effect, the drug loading capacity of the curcumin in the nanoparticles is relatively high, the loading rate of the antibacterial peptide is relatively large, auxiliary materials are safe, the preparation process is simple, the particle size of the nanoparticles is relatively small and uniform, the blood compatibility is good, the nanoparticles can be used for treating infection of drug-resistant bacteria, and the application prospect is wide. The potential of becoming a novel nano antibacterial drug and the clinical application prospect are realized.

The invention relates to an antibacterial peptide LK-KJT-B01 for gram-negative bacterium infection and a preparation method and application thereof.The antibacterial peptide LK-KJT-B01 can be extracted from bacillus laterosporus, has the advantages of being broad in spectrum, safe, free of toxicity and the like, has a very strong bactericidal effect on gram-negative bacterium infection, and can be used for preparing antibacterial peptide LK-KJT-B01 for gram-negative bacterium infection. Particularly, the sensitivity to carbapenem-resistant gram-negative bacteria is high, and the application prospect is very wide.

The invention relates to unique peptide sequences with high antimicrobial activity and stability as well as low hemolytic activity, generated by a unique strategy defined by determining the specific motifs of peptides with high antimicrobial activity in the antimicrobial peptide database by a structure-activity relationship based approach and combining the determined motifs with a localization-based approach taking into account the N-terminal, C-terminal or center position in the peptide.

The invention discloses an antibacterial peptide induced by sulfate reducing bacteria and application of the antibacterial peptide, and belongs to the technical field of marine corrosion protection. The amino acid sequence of the SRB-induced antibacterial peptide is VAHKRLVVGTR, and the SRB-induced antibacterial peptide has efficient antibacterial activity on corrosive bacteria such as SRB and has the advantages of being environmentally friendly and not prone to inducing the bacteria to generate drug resistance, and the problems that an existing antibacterial peptide is insufficient in corrosive bacteria specificity, poor in environmental stability and the like are solved; meanwhile, peptide chains are short, synthesis is easy, and the production cost is low; the compound corrosion inhibition bactericide formed by combining the compound with a chemical bactericide can significantly improve the antibacterial and antiseptic effects, saves the dosage of the chemical bactericide, effectively solves the problems of bacterial drug resistance, high cost, environmental pollution and other pain points caused by abuse of the existing bactericide, and is suitable for the fields of marine microbial corrosion protection and the like.

The invention belongs to the technical field of biological high-throughput data analysis, and particularly relates to a method, a system, equipment and a medium for predicting inhibition specificity of an antibacterial peptide to gram-positive bacteria and gram-negative bacteria, the method comprises the following steps: acquiring an antibacterial peptide sequence with known activity, and respectively sorting antibacterial peptide data sets with gram-positive bacteria activity and gram-negative bacteria activity; on the basis of the obtained antibacterial peptide sequence, three feature models are constructed, the three feature models are trained and predicted through multiple machine learning algorithms, and the optimal model is screened through cross validation. According to the method, by introducing a multi-source feature construction and multi-model integration evaluation mechanism, the limitation that a traditional method depends on a single feature or a single algorithm is broken through, and accurate prediction of the activity difference of the antibacterial peptides between gram-positive bacteria and gram-negative bacteria can be achieved. Besides, the method not only can be used for classifying and predicting the activity of known antibacterial peptides, but also can be used for excavating potential novel antibacterial peptides which are not recognized in the past and have specific activity, and has a wide application prospect.

Embodiments of the disclosure include methods and compositions related to antimicrobial applications. In particular embodiments, the compositions comprise peptides for antimicrobial applications that are also found in tick attachment glue. In some embodiments, the disclosure concerns methods to treat bacterial, viral and fungal infections in an animal or insect and / or to clean an environment or matter of any kind.

The present invention relates to the technical field of antimicrobial peptides, and discloses a Doudnabacteria antimicrobial peptide and its application. The amino acid sequence of the antimicrobial peptide is shown in SEQ ID NO: 1. The antimicrobial peptide obtained by the present invention has a good antibacterial effect on Staphylococcus aureus, Klebsiella pneumoniae, Listeria monocytogenes, and Acinetobacter baumannii.

This invention discloses a *Bacillus laterosporus* strain for the stable production of nonribosomal cyclic lipolipeptide and its construction method. The M1A gene in the Bogorol biosynthetic gene cluster is knocked out using CRISPR-Cas9 technology to block the Bogorol pathway, which competes with Brevicidine for substrates. Subsequently, the pMCCas9-S48 plasmid, which integrates S8 and S41 sgRNAs and their homologous arms as repair templates, is used to simultaneously knock out S8 and S41 family peptidase genes, preventing the expression of extracellular degradation enzymes. This invention also provides the application of the above strain in the preparation of nonribosomal cyclic lipolipeptide of Brevicidine and a method for preparing the nonribosomal cyclic lipolipeptide. This strain, through reducing metabolic competition and preventing product degradation, exhibits rapid cell growth, stable production of Brevicidine, and significantly reduced degradation rate, resulting in a 66.13% increase in fermentation yield compared to the unmodified strain. This invention solves the problems of degradation and unstable yield in the production of Brevicidine, provides an effective technical approach for the industrial production of Brevicidine, and provides important technical support for the development and application of novel antimicrobial peptide drugs.

Provided herein are methods for identifying antimicrobial peptides deriving from extinct proteomes using a multitask deep learning approach. Also disclosed are antimicrobial peptides, and methods of treating an antimicrobial infection comprising contacting the infection with the present antimicrobial peptides. Also disclosed are methods of treating a microbial infection comprising administering to a subject in need thereof a pharmaceutically effective amount of an antimicrobial peptide according to the present disclosure.

Described herein are Staphylococcal toxins inspired peptides (STIPs) with antimicrobial activity, compositions and kits comprising the peptides, and use of the peptides to treat microbial infections. Also described are methods of inhibiting or preventing the growth of a microorganism, and methods of treating microbial infections such as bacterial infections including MRSA, and fungal infections including C. albicans.

The present invention discloses a method and system for identifying antimicrobial peptides based on a sequence-structure dual-channel neural network. The method splices amino acid features extracted by ProtT5 with amino acid-level manual features to obtain peptide embeddings, which are then passed to a sequence channel composed of multiple Transformer blocks to extract the peptide's sequence features. The method also uses ESM-Fold to predict the three-dimensional structure of the peptide to construct an adjacency graph. The amino acid features obtained by ESM-2 are used as node features of the adjacency graph. Layer-by-layer extraction and enhancement are performed by fusing multi-head graph attention, residual networks, layer normalization, and a structural channel of a feedforward neural network. Sequence features and structural features are then max-pooled and spliced ​​for antimicrobial peptide prediction. The present invention adopts a multi-feature fusion strategy, simultaneously introduces the three-dimensional structural information of the antimicrobial peptide, and utilizes a dual-channel architecture to fuse sequence and structural features, effectively improving the accuracy of antimicrobial peptide recognition.

The invention discloses a high-throughput screening method for antibacterial peptides capable of being prepared into medicines based on multi-dimensional attribute evaluation, belongs to the cross technical field of bioinformatics and innovative medicine research and development, and is suitable for an input candidate antibacterial peptide sequence set. The method comprises the following steps: carrying out antibacterial activity preliminary prediction and sorting operation on an input candidate sequence aiming at a target pathogen so as to obtain a pre-selected subset; performing multi-dimensional evaluation on the pre-selected subset based on a plurality of preset druggability indexes; in the evaluated sequence, the minimum inhibitory concentration (MIC) value of the sequence is further predicted, and finally the target antibacterial peptide is screened out according to the predicted MIC value. According to the method, through a layered and multi-module linkage screening process and application of the optimized machine learning model, the antibacterial peptide with high activity and good patent medicine potential is efficiently and accurately screened out, the screening efficiency is remarkably improved, and the research and development risk is reduced.

According to the present invention, a technique which enables the production of an antimicrobial peptide Persulcatusin at reduced cost has been developed. A plant cell into which a polynucleotide sequence that encodes a gene for a protease to which a signal peptide localized in an intercellular organelle is added and a polynucleotide sequence that encodes a Persulcatusin fusion protein to which a signal peptide localized in an intracellular organelle different from the aforementioned intracellular organelle is added are introduced is produced, wherein, in the Persulcatusin fusion protein, a sequence that is cleavable with the protease is disposed between Persulcatusin and a protein that fuses to the Persulcatusin. Thus, Persulcatusin, which can serve as a therapeutic agent for bovine mastitis, can be produced at low cost.

This invention provides the application of Hepcidin-1 (Ms-Hepcidin-1) and its synthetic polypeptide in the preparation of antiviral drugs for largemouth bass. The invention reveals that the Ms-Hepcidin-1 synthetic polypeptide exhibits significant antiviral activity and is a novel antimicrobial peptide with the function of inhibiting fish viruses. Both the Ms-Hepcidin-1 synthetic polypeptide and its recombinant eukaryotic expression vector can significantly reduce the transcriptional levels of MCP, MMP, and DNMT genes and the MCP protein level of LMBV, inhibit LMBV replication, and reduce viral titers, demonstrating significant antiviral activity against fish viruses. Furthermore, the polypeptide synthesized from Ms-Hepcidin-1 is simple to prepare, low in cost, safe to use, and easy to store. As a novel antiviral functional gene product, it can be applied to the preparation of antiviral drugs for fish, which is of great significance for the prevention and control of iridovirus disease in fish.

The present invention relates to a novel peptide derived from Hirudo nipponia, which has antimicrobial activity, antibiofilm activity, antibiotic sensitivity–enhancing activity, and anti-inflammatory activity. With antimicrobial activity, antibiofilm activity, antibiotic sensitivity–enhancing activity, and anti-inflammatory activity, the novel peptide derived from Hirudo nipponia according to the present invention can be advantageously applied as a material for various purposes in the pharmaceutical field, food field, cosmetic field, and agriculture and livestock industries. In addition, the novel antimicrobial peptide derived from Hirudo nipponia according to the present invention increases the sensitivity of antibiotics to multidrug-resistant bacteria, thereby markedly enhancing antimicrobial activity against multidrug-resistant bacteria when used in combination with antibiotics. Accordingly, a composition of the present invention comprising the peptide as an active ingredient can be advantageously used as a pharmaceutical composition for enhancing antibiotic sensitivity. In particular, the novel peptide of the present invention has very low hemolytic activity and cytotoxicity, and thus is safe for the human body. Moreover, the novel peptide of the present invention not only has excellent activity even under a high salt concentration, but also maintains activity even in a serum environment, thereby having high stability when applied in vivo.

This invention provides a multi-component resource utilization process for black soldier fly larvae larvae slurry, relating to the field of insect resource utilization technology. The process sequentially includes raw material pretreatment, compound enzymatic hydrolysis, lactic acid bacteria fermentation, stratified separation, and targeted treatment of each component. The compound enzymatic hydrolysis uses a combined enzyme system of trypsin and papain, and the lactic acid bacteria fermentation uses *Lactobacillus plantarum*. The fermentation parameters are: carbon-to-nitrogen ratio of 20:1, material-to-liquid ratio of 4:1 (w / v), pH of 6.5, fermentation temperature of 37°C, and fermentation time of 24 hours. This process achieves the synergistic recovery and high-value utilization of all components of black soldier fly larvae. A single process can simultaneously produce four types of products: insect oil, water-soluble fertilizer, antimicrobial peptide additives, and antibiotic-free aquatic feed ingredients, significantly improving resource utilization and industrial added value. The entire process involves no organic solvent addition, employs low-temperature enzyme inactivation to reduce energy consumption, is environmentally friendly and energy-saving, and avoids antibiotic resistance issues.

The invention relates to bacillus velezensis antibacterial peptide and application thereof in food preservation, and relates to the technical field of biology. The amino acid sequence of the antibacterial peptide is as shown in SEQ ID NO: 1. The antibacterial peptide provided by the invention is brand new in structure, and a database of the antibacterial peptide is enriched; the antibacterial peptide can effectively inhibit normal growth of fusarium oxysporum, is wide in antibacterial spectrum and has potential development and application prospects. The antibacterial peptide can effectively delay the refrigeration shelf life of pitaya, and has a better application prospect in the aspect of fruit preservation.

The present invention relates to a peptide comprising an amino acid sequence of SEQ ID NO: 1 having antimicrobial activity, a polynucleotide encoding the peptide of the invention, a composition comprising the peptide of the invention and methods using the peptide or composition of the invention.

This invention relates to Bacillus vesiculosus antimicrobial peptides and their application in food preservation, belonging to the field of biotechnology. The amino acid sequence of the antimicrobial peptide is shown in SEQ ID NO: 1. The antimicrobial peptide provided by this invention is a novel antimicrobial peptide in structure, enriching the antimicrobial peptide database; this antimicrobial peptide can effectively inhibit the normal growth of Fusarium oxysporum, has a broad antimicrobial spectrum, and has potential development and application prospects; this antimicrobial peptide can effectively extend the refrigerated shelf life of dragon fruit, and has good application prospects in fruit preservation.