Scalp micro-ecology regulation washing and care composition containing lactobacillus metabiotics

Abstract

The invention discloses a scalp micro-ecological regulation washing and care composition containing lactobacillus metagen, and particularly relates to the technical field of daily chemical washing and care products. The washing and care composition comprises the following specific components in percentage by weight: 3%-8% of lactobacillus metagen, 1%-3% of prebiotics, 0.05%-0.2% of natural antibacterial peptide, 0.5%-1.5% of a ceramide compound, 0.3%-0.7% of a pH regulator, 0.5%-2% of a microcapsule wrapping agent, 0.2%-0.6% of a natural preservative and the balance of deionized water, wherein the total percentage is 100%. According to the invention, various components are scientifically compounded, so that the washing and care composition has an excellent effect, the lactobacillus retrogen, the natural antibacterial peptide and the natural preservative cooperate to inhibit bacteria, the diameter of an inhibition zone for common pathogenic bacteria is 14-20mm, and the prebiotics and the lactobacillus retrogen regulate scalp micro-ecology, so that the flora diversity index is increased by 30-45%; the ceramide compound and dipotassium glycyrrhizinate cooperate to reduce the TEWL value by 30%-50%, the scalp barrier is repaired, and the product is good in stability, safe, reliable and capable of comprehensively caring scalp health.

Description

Technical Field

[0001] The present invention relates to the technical field of daily chemical cleaning products, and in particular to a scalp microecological regulating cleaning composition containing lactobacillus postbiotics. Background Art

[0002] As people pay more and more attention to scalp health, the demand for scalp care products is growing and more emphasis is placed on efficacy and safety. Traditional scalp care products mostly focus on cleaning and basic hair care functions. However, modern research shows that scalp microecological balance is crucial to scalp health, which makes it increasingly difficult for traditional products to meet people's needs.

[0003] On the one hand, although common sulfate surfactants have strong cleaning power, they will destroy the natural barrier of the scalp, causing dry and sensitive scalp. Long-term use may also affect the balance of scalp microecology. Although silicone oil ingredients can make hair smooth, they will accumulate on the scalp and hair surface, clogging hair follicles and hindering the normal metabolism of the scalp. On the other hand, the scalp is easily affected by various factors, such as environmental pollution, life stress, and bad living habits, which can lead to imbalance of scalp microecology and problems such as dandruff, itching, and inflammation. Some products currently on the market that claim to have microecological regulation functions either have ineffective ingredients or poor stability, and cannot truly achieve effective regulation and long-term care of the scalp microecology.

[0004] In addition, once the scalp's barrier function is damaged, transepidermal water loss increases, the scalp becomes dry and flaky, and the scalp's microecological environment is further damaged. Currently, most shampoo and conditioner products have limited effects in repairing the scalp barrier. Therefore, developing a shampoo and conditioner composition that does not contain harmful ingredients, can effectively regulate the scalp microecology, repair the scalp barrier, and has good stability has become an urgent problem to be solved in the industry. Summary of the Invention

[0005] The main purpose of the present invention is to provide a scalp microecological regulation washing and care composition containing lactobacillus postbiotics, which can effectively solve the problems mentioned in the background technology.

[0006] To achieve the above object, the technical solution adopted by the present invention is: A scalp microecological regulation washing and care composition containing lactobacillus postbiotics, wherein the ingredients include, by weight percentage: lactobacillus postbiotics 3% to 8%, prebiotics 1% to 3%, natural antimicrobial peptides 0.05% to 0.2%, ceramide complexes 0.5% to 1.5%, pH regulators 0.3% to 0.7%, microencapsulation agents 0.5% to 2%, natural preservatives 0.2% to 0.6%, and deionized water increased to 100%; The lactobacillus postbiotics are spray-dried microcapsule particles with an active ingredient retention rate of 85% to 95% and a sustained release time of 8 to 12 hours. The ceramide complex is composed of ceramide NP, AP and EOP in a mass ratio of 1:1:0.5, and the free fatty acid content is 0.1% to 0.5%; The pH value of the composition is 5.7-5.9, which is regulated by a citric acid-sodium citrate buffer system.

[0007] A method for preparing the above-mentioned lactobacillus postbiotics, the method comprising the following steps: S1. Bacteria activation: Lactobacillus was inoculated into MRS medium and cultured at 37°C for 24 hours; S2, fermentation culture: inoculate the activated bacteria into a fermentation medium containing 5% inulin, and ferment at 38°C and pH 5.5 for 60 hours; S3. Post-treatment: The bacteria were removed by centrifugation, and the filtrate was concentrated by ultrafiltration with a molecular weight cut-off of 10 kDa. It was then mixed with sodium alginate-chitosan microcapsules and spray-dried to form particles with a particle size of 10 to 50 μm.

[0008] Preferably, the natural antimicrobial peptide is lactoferrin peptide (LFcinB20), and the weight percentage ratio of the added amount of LFcinB20 to the Lactobacillus postbiotics is 1:50 to 1:30.

[0009] Preferably, the prebiotic is a complex of inulin and chitosan oligosaccharide, with a mass ratio of 2:1 and a molecular weight range of 300 to 1500 Da.

[0010] Preferably, the microcapsule encapsulation agent is a double-layer structure formed by layer-by-layer self-assembly of sodium alginate (1.5% w / v) and chitosan (0.8% w / v), with an encapsulation efficiency of 90% to 95%, and a sustained release rate of 8% to 12% / h in a simulated scalp environment at a temperature of 32°C and a humidity of 85%.

[0011] Preferably, the composition further comprises 0.1% to 0.3% of dipotassium glycyrrhizate, which synergistically acts with the ceramide complex to reduce transepidermal water loss (TEWL) by 40% to 50%.

[0012] Preferably, the natural preservative is caprylyl glycol and parahydroxyacetophenone in a mass ratio of 2:1, with a total addition amount of 0.3% to 0.5%, and the diameter of the inhibition zone for the common scalp pathogens Malassezia globosa and Staphylococcus epidermidis is 15 to 20 mm.

[0013] Preferably, the conductivity of the deionized water is 1-3 μS / cm, and the content of heavy metals (Pb, As, Hg) in the composition complies with EU EC1223 / 2009 standard.

[0014] A method for preparing the above-mentioned scalp microecological regulating cleaning and care composition, the method comprising the following steps: a1. Microcapsule preparation: Lactobacillus postbiotic filtrate is mixed with sodium alginate solution, and the microcapsule primary particles are formed by high-voltage electrostatic spraying. The microcapsules are then immersed in chitosan solution to form a double-layer structure. After drying, the particles are sieved to a particle size of 10-50 μm. a2. Preparation of base solution: Dissolve prebiotics, lactoferrin peptide and ceramide complex in deionized water and stir at 45°C until completely dissolved; a3. Mixing and homogenization: Disperse the microcapsule particles in S1 into the base liquid of S2, add pH adjuster and natural preservative, and homogenize at 2500 rpm for 25 minutes; a4. Final treatment: Detect the pH value and adjust it to 5.8±0.1. Sterilize through a 0.22μm filter membrane and then fill.

[0015] Compared with the prior art, the present invention has the following beneficial effects: The present invention scientifically compounds multiple ingredients to achieve excellent results in the shampoo and conditioner composition. Lactobacillus postbiotics, natural antimicrobial peptides, and natural preservatives synergistically inhibit bacteria, with an inhibition zone diameter of 14 to 20 mm for common pathogens. Prebiotics and Lactobacillus postbiotics regulate the scalp microecology, increasing the flora diversity index by 30% to 45%. Ceramide complexes and dipotassium glycyrrhizate synergize to reduce TEWL values ​​by 30% to 50%, repairing the scalp barrier. The product has excellent stability, with an active ingredient retention rate of 85% to 93% after storage at 40°C for 3 months. It does not contain sulfate surfactants and silicone oil, making it safe and reliable, and comprehensively protecting scalp health. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 The steps of the present invention Figure 1 ; Figure 2 The steps of the present invention Figure 2 . DETAILED DESCRIPTION

[0017] In order to make the technical means, creative features, objectives and effects achieved by the present invention easier to understand, the present invention is further described below in conjunction with specific implementation methods.

[0018] A scalp microecological regulation washing and care composition containing lactobacillus postbiotics, wherein the ingredients include, by weight percentage: lactobacillus postbiotics 3% to 8%, prebiotics 1% to 3%, natural antimicrobial peptides 0.05% to 0.2%, ceramide complexes 0.5% to 5%, pH regulators 0.3% to 0.7%, microencapsulation agents 0.5% to 2%, natural preservatives 0.2% to 0.6%, and deionized water increased to 100%; Among them, the lactobacillus postbiotics are spray-dried microcapsule particles with an active ingredient retention rate of 85% to 95% and a sustained release time of 8 to 12 hours; The ceramide complex is composed of ceramide NP, AP, and EOP in a mass ratio of 1:1:0.5, and the free fatty acid content is 0.1% to 0.5%; The pH value of the composition is 5.7-5.9, which is regulated by a citric acid-sodium citrate buffer system.

[0019] Bacterial strain selection and fermentation medium preparation: The genetically characterized Lactobacillus reuteri DSM17938 strain (carrying the Lre_0165 antimicrobial peptide encoding gene) was used, and the fermentation medium contained 5% low molecular weight inulin (300 Da), 0.5% yeast extract and 0.1% potassium dihydrogen phosphate, pH 5.5.

[0020] Fermentation process control: 38°C anaerobic environment (nitrogen replacement), stirring speed 150 rpm, end point pH 4.2±0.1, fermentation was terminated when the viable bacterial count ≥1×10^9 CFU / mL.

[0021] Post-treatment and microcapsule molding: centrifugation (8000×g, 15 minutes) was performed to remove the bacteria, and the filtrate was concentrated by ultrafiltration to a solid content of 20%, mixed with sodium alginate-chitosan solution, and spray-dried (inlet air 160°C, outlet air 80°C) to obtain microcapsule particles with a particle size of 10-50 μm.

[0022] Key raw material specifications and testing methods: Lactoferrin peptide (LFcinB20): purity ≥95% (HPLC detection, C18 column, mobile phase acetonitrile-water gradient elution), molecular weight 3.2kDa (MALDI-TOF mass spectrometry verification).

[0023] Ceramide complex: free fatty acid content ≤0.5% (GC-MS determination), lipid lamellar structure density determined by small-angle X-ray scattering (SAXS) (interlayer spacing ≤4.8 nm).

[0024] The preparation method of lactobacillus postbiotics comprises the following steps: S1. Bacteria activation: Lactobacillus was inoculated into MRS medium and cultured at 37°C for 24 hours; S2, fermentation culture: inoculate the activated bacteria into a fermentation medium containing 5% inulin, and ferment at 38°C and pH 5.5 for 60 hours; S3. Post-treatment: The bacteria were removed by centrifugation, and the filtrate was concentrated by ultrafiltration (molecular weight cut-off 10 kDa) and then mixed with sodium alginate-chitosan microcapsules and spray-dried to form particles with a particle size of 10 to 50 μm.

[0025] Specific implementation methods of strain activation and fermentation optimization: Activation of bacterial strains: inoculate the freeze-dried bacterial strain into MRS liquid medium (containing 0.05% L-cysteine), culture at 37°C for 24 hours, and let the OD600 reach 1.8-2.0. After activation, centrifuge the bacterial solution (5000×g, 10 minutes), wash the bacterial slurry three times with physiological saline, and set aside.

[0026] Fermentation process monitoring: Real-time monitoring of dissolved oxygen (DO≤5%), pH (automatically adding 5% NaOH to maintain 5.5±0.1) and metabolites (lactic acid ≥8g / L, acetic acid ≤1g / L).

[0027] Fermentation endpoint determination: Fermentation is terminated when the number of viable bacteria is ≥1×10^9 CFU / mL.

[0028] Microcapsule spray drying parameters: inlet air temperature 160 °C, outlet air temperature 80 °C, atomization pressure 0.3 MPa, and collected microcapsule particles with a particle size of 10 to 50 μm (SEM verification).

[0029] The natural antimicrobial peptide is lactoferrin peptide (LFcinB20), and the weight percentage ratio of the added amount of LFcinB20 to the lactobacillus postbiotics is 1:50 to 1:30.

[0030] The verification method for the synergistic ratio of lactoferrin peptide and postbiotics is: Antimicrobial peptide activity test: The agar diffusion method was used: 0.1% LFcinB20 solution was added dropwise to a Sabouraud agar plate containing Malassezia globosa and incubated at 37°C for 48 hours. The diameter of the inhibition zone was 15-20 mm (compared to 10-12 mm for commercially available antimicrobial peptides).

[0031] Verification of synergistic effect: When the ratio of LFcinB20 to postbiotics is 1:50, the inhibition rate against Staphylococcus epidermidis increases to 95% (the inhibition rate of a single postbiotic is 70%).

[0032] Stability test: The LFcinB20-containing composition was accelerated stored at 40°C for 3 months, and the activity retention rate was ≥85% (HPLC detection), compared with 60% for the sample without added antimicrobial peptide.

[0033] Prebiotics are a complex of inulin and chitosan oligosaccharides, with a mass ratio of 2:1 and a molecular weight range of 300 to 1500 Da.

[0034] Prebiotic molecular weight screening and functional verification methods: Optimization of inulin and chitosan oligosaccharide compounding: Molecular weight screening: Low molecular weight inulin (300Da) promotes the proliferation of lactobacilli (OD600 increased by 30%), and high molecular weight inulin (1500Da) enhances scalp barrier repair (TEWL value reduced by 25%).

[0035] When the compound ratio of inulin:chitosan oligosaccharide = 2:1, the diversity of scalp flora increased by 40% (16SrRNA sequencing).

[0036] Solubility test: Dissolve the compound prebiotics in 45°C deionized water with a stirring speed of 500 rpm. The dissolution time is ≤10 minutes (compared to the dissolution time of the single ingredient, which is ≥20 minutes).

[0037] The microcapsule encapsulation agent is a double-layer structure formed by layer-by-layer self-assembly of sodium alginate (5% w / v) and chitosan (0.8% w / v). The encapsulation efficiency is 90% to 95%, and the sustained release rate is 8% to 12% / h in a simulated scalp environment (temperature 32°C, humidity 85%).

[0038] Optimization of microcapsule sustained-release performance: Double-layer structure construction: Preparation of primary particles: The postbiotic filtrate was mixed with sodium alginate solution and formed into droplets with a particle size of 20-30 μm by high-voltage electrostatic spraying (voltage 15 kV, flow rate 2 mL / min).

[0039] Chitosan coating: The primary particles were immersed in chitosan solution (pH 5.0) for 10 min to form a shell layer with a thickness of 1-2 μm (TEM observation).

[0040] Sustained release kinetics test: Simulated scalp environment (32°C, 85% humidity): Release rate within 8 hours ≤ 12%, cumulative release rate within 24 hours ≥ 90% (determined by UV-Vis method).

[0041] The scalp microecological regulation washing and care composition containing lactobacillus postbiotics also includes 0.1% to 0.3% of dipotassium glycyrrhizate, which acts synergistically with the ceramide complex to reduce transepidermal water loss (TEWL value) by 40% to 50%.

[0042] Synergistic barrier repair of dipotassium glycyrrhizate and ceramide: TEWL value test: Clinical trial: 30 subjects used a composition containing 0.3% dipotassium glycyrrhizate for 28 days, and the TEWL value decreased from 15.2g / (m²·h) to 8.7g / (m²·h), a decrease of 43% (compared with a 25% decrease in the group without addition).

[0043] Electron microscopy observation: The ceramide complex and dipotassium glycyrrhizate act synergistically, making the lipid lamellar structure of the stratum corneum more tightly arranged (the interlayer spacing is reduced by 35%).

[0044] The natural preservative is a mixture of caprylyl glycol and parahydroxyacetophenone in a mass ratio of 2:1, with a total addition amount of 0.3% to 0.5%. The diameter of the inhibition zone for common scalp pathogens (Malassezia globosa and Staphylococcus epidermidis) is 15 to 20 mm.

[0045] Verification of antibacterial properties of natural preservatives: Optimization of compound preservative ratio: When the ratio of 2:1 is 2, the minimum inhibitory concentration (MIC) against Malassezia globosa is 0.2%, compared with the MIC of a single component ≥ 0.5%.

[0046] Irritation test: Patch test (n=50) showed a positive reaction rate of 0%, in line with ISO10993-10 standard.

[0047] The conductivity of the deionized water is 1 to 3 μS / cm, and the content of heavy metals (Pb, As, Hg) in the composition complies with EU EC1223 / 2009 standards.

[0048] Deionized water purification and heavy metal control: Ultrafiltration system parameters: Using reverse osmosis (RO) combined with electrodeionization (EDI) technology, the produced water conductivity is ≤3μS / cm, and the heavy metal residues are (Pb≤0.1ppm, As≤0.05ppm, Hg≤0.01ppm).

[0049] Water quality verification: Weekly sampling and testing, in compliance with EU EC1223 / 2009 standards.

[0050] A method for preparing a scalp microecological regulating cleaning and care composition comprises the following steps: a1. Microcapsule preparation: Lactobacillus postbiotic filtrate is mixed with sodium alginate solution, and the microcapsule primary particles are formed by high-voltage electrostatic spraying. The microcapsules are then immersed in chitosan solution to form a double-layer structure. After drying, the particles are sieved (particle size 10-50 μm). The high-voltage electrostatic spray parameters were: voltage 15 kV, liquid flow rate 2 mL / min, nozzle aperture 0.4 mm, and collection distance 15 cm. Chitosan solution treatment: Chitosan solution concentration was 0.8% w / v (pH 5.0), and the primary particles were immersed for 10 minutes to form a shell layer with a thickness of 1-2 μm (TEM verification); Drying process: Spray drying is adopted (inlet air temperature 160℃, outlet air temperature 80℃), and the particle size after sieving is 10-50μm (D90 distribution ≤5%, detected by laser particle size analyzer).

[0051] a2. Preparation of base solution: Dissolve prebiotics, lactoferrin peptide and ceramide complex in deionized water and stir at 45°C until completely dissolved; Dissolution conditions: 45°C constant temperature water bath, stirring speed 500 rpm, dissolution time ≤ 10 minutes (no suspended particles observed visually); Prebiotic pretreatment: Inulin and chitosan oligosaccharide are premixed in a mass ratio of 2:1 and then added to avoid excessive local concentration.

[0052] a3. Mixing and homogenization: Disperse the microcapsule particles in S1 into the base liquid of S2, add pH adjuster and natural preservative, and homogenize (2500 rpm, 25 minutes); Homogenizing equipment: High shear homogenizer (Silverson L5M-A) with a homogenizing head gap of 0.5 mm; Temperature control: Maintain the system temperature ≤35°C throughout the process to prevent microcapsules from rupturing (verified by thermal sensitivity test).

[0053] a4. Final treatment: Detect the pH value and adjust it to 5.8±0.1. Sterilize through a 0.22μm filter membrane and then fill.

[0054] pH adjuster: Use 0.1% citric acid solution or 0.05% sodium hydroxide solution to adjust the pH drop by drop, adding every 2 minutes and stirring; Sterile filtration: using polyethersulfone (PES) material 0.22μm filter membrane, filtration pressure ≤ 0.3MPa; Filling environment: Sterile operating table (cleanliness level ISO 5), sealed immediately after filling (oxygen content ≤ 0.5%).

[0055] Example 1, combined with the preparation process of the above-mentioned scalp microecological regulation washing and care composition containing lactobacillus postbiotics, during the raw material preparation process, the components are weighed according to the weight ratio: lactobacillus postbiotics 3%, prebiotics 1%, natural antimicrobial peptide (LFcinB20) 0.05%, ceramide complex 0.5%, pH regulator 0.3%, microcapsule encapsulation agent 0.5%, natural preservative 0.2%, dipotassium glycyrrhizate 0.1%, and deionized water is added to 100%.

[0056] Example 2, based on Example 1, the weight ratio of each component was changed to: 5% lactobacillus postbiotics, 2% prebiotics, 0.1% natural antimicrobial peptide (LFcinB20), 2% ceramide complex, 0.5% pH regulator, 1% microcapsule encapsulation agent, 0.3% natural preservative, 0.2% dipotassium glycyrrhizate, and deionized water was added to 100%.

[0057] Example 3, based on Example 1, the weight ratio of each component was changed to: 6% lactobacillus postbiotics, 2.5% prebiotics, 0.15% natural antimicrobial peptide (LFcinB20), 3% ceramide complex, 0.6% pH regulator, 1.5% microcapsule encapsulation agent, 0.4% natural preservative, 0.25% dipotassium glycyrrhizate, and deionized water was added to 100%.

[0058] Example 4, based on Example 1, the weight ratio of each component was changed to: 7% lactobacillus postbiotic, 2.8% prebiotic, 0.18% natural antimicrobial peptide (LFcinB20), 4% ceramide complex, 0.65% pH regulator, 1.8% microcapsule encapsulation agent, 0.5% natural preservative, 0.28% dipotassium glycyrrhizate, and deionized water was added to 100%.

[0059] Example 5, based on Example 1, the weight ratio of each component was changed to: 8% lactobacillus postbiotics, 3% prebiotics, 0.2% natural antimicrobial peptide (LFcinB20), 5% ceramide complex, 0.7% pH regulator, 2% microcapsule encapsulation agent, 0.6% natural preservative, 0.3% dipotassium glycyrrhizate, and deionized water was added to 100%.

[0060] Comparative Example 1: Based on Example 2, the lactobacillus postbiotic was removed, and the proportions of other raw materials were not changed. A composition was prepared according to the above raw materials and prepared for the next step of testing.

[0061] Comparative Example 2: Based on Example 3, the natural antimicrobial peptide (LFcinB20) was removed, and the proportions of other raw materials remained unchanged. A composition was prepared based on the above raw materials and prepared for the next step of testing.

[0062] Comparative Example 3: Based on Example 4, the ceramide complex was removed, and the proportions of the remaining raw materials remained unchanged. A composition was prepared based on the above raw materials and prepared for the next step of testing.

[0063] Comparative Example 4: Based on Example 5, a common surfactant was used instead of the natural preservative, and the proportions of other raw materials were not changed. A composition was prepared according to the above raw materials and prepared for the next step of testing.

[0064] The compositions prepared in Examples 1 to 5 and Comparative Examples 1 to 4 were subjected to the following experiments to clarify the differences in antibacterial performance, scalp flora diversity, transepidermal water loss, and stability of each composition: Antibacterial performance test: The diameter of the inhibition zone for common scalp pathogens (Malassezia globosa and Staphylococcus epidermidis) was determined using the agar diffusion method. The prepared shampoo and conditioner solutions with different formulas were added dropwise to an agar plate containing the corresponding pathogens. The size of the inhibition zone was measured after incubation at 37°C for 48 hours.

[0065] Scalp flora diversity test: Using 16S rRNA sequencing technology, the diversity index of the scalp flora after using different formulas of shampoo and conditioner compositions was analyzed. A certain number of volunteers were selected and scalp samples were collected for testing after using the products for a period of time.

[0066] Transepidermal Water Loss (TEWL) testing: Professional instruments were used to measure the changes in TEWL values ​​of the subjects' skin after using different formulas of cleansing and conditioner compositions. Volunteers used the products continuously for 28 days, and the TEWL values ​​were measured before and after use.

[0067] Stability test: The cleaning compositions of different formulas were stored at 40°C for 3 months, and the retention rate of active ingredients (such as lactobacillus postbiotics, natural antimicrobial peptides, etc.) was detected by HPLC.

[0068] Specifically, the test results of the compositions prepared in Examples 1 to 5 and Comparative Examples 1 to 4 are shown in the table below: Combining the above table, we can get the following performance test results: Antibacterial Performance: The examples demonstrated a relatively large inhibition zone diameter against Malassezia globosa and Staphylococcus epidermidis, ranging from 14 to 20 mm, demonstrating excellent antibacterial efficacy. Comparative Example 1, due to the lack of Lactobacillus postbiotics, exhibited a significantly smaller inhibition zone diameter. Furthermore, Comparative Example 4, which replaced the natural preservative with a conventional surfactant, also exhibited a decrease in antibacterial performance. This demonstrates the crucial role of synergy between Lactobacillus postbiotics, natural antimicrobial peptides, and natural preservatives in inhibiting common scalp pathogens.

[0069] Scalp flora diversity: The scalp flora diversity index of the embodiment increased by 30% to 45%, effectively promoting the balance of the scalp microecology. Due to the lack of Lactobacillus postbiotics in Comparative Example 1, the improvement rate was only 10%, indicating that Lactobacillus postbiotics made a significant contribution to increasing flora diversity.

[0070] TEWL Value: The Examples achieved a TEWL reduction rate of between 30% and 50%, effectively reducing transepidermal water loss and protecting the scalp barrier. Comparative Example 3, which lacked the ceramide complex, showed a significantly weaker TEWL reduction of only 30%, demonstrating that the synergistic effect of the ceramide complex and dipotassium glycyrrhizate is essential for repairing the scalp barrier.

[0071] Stability: After three months of accelerated storage at 40°C, the active ingredient retention rate in the examples was high, ranging from 85% to 93%, indicating good product stability. Comparative Examples 1 and 2, due to the lack of Lactobacillus postbiotics and natural antimicrobial peptides, had lower active ingredient retention rates of 60% and 70%, respectively, demonstrating the importance of these ingredients in maintaining product stability.

[0072] Based on the experimental results, Example 5 performed the best among the many formulas. In terms of antibacterial properties, the diameters of the inhibition zones against Malassezia globosa and Staphylococcus epidermidis reached 20 mm and 19 mm, respectively, effectively inhibiting common scalp pathogens, better maintaining a healthy scalp environment, and reducing scalp problems caused by bacterial growth. Its scalp flora diversity index increased by 45%, showing significant effects in regulating the scalp microecology, increasing flora richness and diversity, and contributing to the establishment of a stable and healthy scalp microbial community. In terms of repairing the scalp barrier function, the TEWL value decreased by 50%, effectively reducing transepidermal water loss and enhancing the scalp's moisturizing ability and barrier integrity. In stability testing, the active ingredient retention rate reached 93%, and it still maintained a high level of activity after three months of accelerated storage at 40°C, indicating excellent product quality stability.

[0073] The basic principles, main features, and advantages of the present invention are shown and described above. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are merely illustrative of the principles of the present invention. Various changes and modifications may be made to the present invention without departing from the spirit and scope of the present invention. Such changes and modifications are intended to fall within the scope of the present invention. The scope of protection claimed in the present invention is defined by the appended claims and their equivalents.

Claims

1. A scalp microecological regulation washing and care composition containing lactobacillus postbiotics, characterized in that: The components include, by weight percentage: 3% to 8% lactobacillus postbiotics, 1% to 3% prebiotics, 0.05% to 0.2% natural antimicrobial peptides, 0.5% to 1.5% ceramide complexes, 0.3% to 0.7% pH regulators, 0.5% to 2% microcapsule encapsulation agents, 0.2% to 0.6% natural preservatives, and deionized water increased to 100%; The lactobacillus postbiotics are spray-dried microcapsule particles with an active ingredient retention rate of 85% to 95% and a sustained release time of 8 to 12 hours. The ceramide complex is composed of ceramide NP, AP and EOP in a mass ratio of 1:1:0.5, and the free fatty acid content is 0.1% to 0.5%; The pH value of the composition is 5.7-5.9, which is regulated by a citric acid-sodium citrate buffer system.

2. The scalp microecological regulation washing and care composition containing lactobacillus postbiotics according to claim 1, characterized in that: The preparation method of the lactobacillus postbiotic comprises the following steps: S1. Bacteria activation: Lactobacillus was inoculated into MRS medium and cultured at 37°C for 24 hours; S2, fermentation culture: inoculate the activated bacteria into a fermentation medium containing 5% inulin, and ferment at 38°C and pH 5.5 for 60 hours; S3. Post-treatment: The bacteria were removed by centrifugation, and the filtrate was concentrated by ultrafiltration with a molecular weight cut-off of 10 kDa. It was then mixed with sodium alginate-chitosan microcapsules and spray-dried to form particles with a particle size of 10 to 50 μm.

3. The scalp microecological regulation washing and care composition containing lactobacillus postbiotics according to claim 1, characterized in that: The natural antimicrobial peptide is lactoferrin peptide, and the weight percentage ratio of the added amount of lactoferrin peptide to the lactobacillus postbiotics is 1:50 to 1:

30.

4. The scalp microecological regulation cleaning and care composition containing lactobacillus postbiotics according to claim 1, characterized in that: The prebiotic is a complex of inulin and chitosan oligosaccharide, with a mass ratio of 2:1 and a molecular weight range of 300 to 1500 Da.

5. The scalp microecological regulation cleaning and care composition containing lactobacillus postbiotics according to claim 1, characterized in that: The microcapsule encapsulation agent is a double-layer structure formed by layer-by-layer self-assembly of sodium alginate (1.5% w / v) and chitosan (0.8% w / v), and is placed in a simulated scalp environment at a temperature of 32° C. and a humidity of 85%.

6. The scalp microecological regulation cleaning and care composition containing lactobacillus postbiotics according to claim 1, characterized in that: The composition further comprises 0.1% to 0.3% of dipotassium glycyrrhizate.

7. The scalp microecological regulation cleaning and care composition containing lactobacillus postbiotics according to claim 1, characterized in that: The natural preservative is a mixture of caprylyl glycol and p-hydroxyacetophenone in a mass ratio of 2:1, with a total addition amount of 0.3% to 0.5%.

8. The scalp microecological regulation cleaning and care composition containing lactobacillus postbiotics according to claim 1, characterized in that: The conductivity of the deionized water is 1-3 μS / cm.

9. A method for preparing the scalp microecological regulating cleaning and care composition according to any one of claims 1 to 8, characterized in that: The following steps are involved: a1. Microcapsule preparation: Lactobacillus postbiotic filtrate is mixed with sodium alginate solution, and the microcapsule primary particles are formed by high-voltage electrostatic spraying. The microcapsules are then immersed in chitosan solution to form a double-layer structure. After drying, the particles are sieved to a particle size of 10-50 μm. a2. Preparation of base solution: Dissolve prebiotics, lactoferrin peptide and ceramide complex in deionized water and stir at 45°C until completely dissolved; a3. Mixing and homogenization: Disperse the microcapsule particles in S1 into the base liquid of S2, add pH adjuster and natural preservative, and homogenize at 2500 rpm for 25 minutes; a4. Final treatment: Detect the pH value and adjust it to 5.8±0.

1. Sterilize through a 0.22μm filter membrane and then fill.

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