18 results about "Protein tag" patented technology

The present disclosure provides a fusion protein, a conjugate containing the same, a kit and use, belonging to the technical field of immunodiagnosis. The fusion protein of the present disclosure comprises a protein tag and a protein part, the fusion protein containing the protein tag has improved activity or specificity, and the fusion protein is used for immunodetection, which can improve the sensitivity or specificity of immunodetection.

The TnpB nuclease is any one of (A1), (A2), and (A3): (A1) the TnpB nuclease having the amino acid sequence shown in SEQ ID NO: 1; (A2) a protein having more than 80% identity with the TnpB nuclease and having an identical biological function as the TnpB nuclease, where the protein is obtained by substituting, deleting, and / or adding one or more amino acid residues to the amino acid sequence of the TnpB nuclease; and (A3) a fusion protein obtained by fusing a protein tag to an N-terminus and / or a C-terminus of (A1) or (A2). The TnpB nuclease possesses both cis-cleavage activity and trans-cleavage activity and has broad application prospects in the fields of nucleic acid detection and gene editing.

The application discloses a plant type and yield related GmMBR2 protein and a coding gene and application thereof, and belongs to the field of plant breeding, and relates to a plant type and yield related GmMBR2 protein and a coding gene and application thereof. The protein of the application is any one of the following: A1) a protein with an amino acid sequence shown in SEQ ID No. 1; A2) a protein obtained by substitution, deletion and / or addition of amino acid residues on the protein of A1) and having more than 80% identity with the protein shown in A1) and having the same function; and A3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1) or A2). Experiments prove that the protein GmMBR2 can regulate plant type and yield, and has important theoretical significance for soybean breeding.

The application discloses a cadmium tolerance related protein IbCSase of sweet potato and an encoding gene and application thereof, and belongs to the technical field of biotechnology, and particularly relates to the cadmium tolerance related protein IbCSase of sweet potato and the encoding gene and application thereof. The protein IbCSase is any one of the following: A1) a protein with an amino acid sequence as shown in SEQ ID No. 1; A2) a protein with more than 80% identity with the protein shown in A1) and the same function, which is obtained by substitution, deletion and / or addition of amino acid residues of the protein of A1); and A3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1) or A2). By introducing the IbCSase encoding gene into wild type sweet potato, the tolerance of the plant to heavy metal cadmium can be improved, the IbCSase encoding gene can be applied to sweet potato breeding, and has wide application space and market prospect in the agricultural field.

The application discloses an anti-disease related protein OsMED16, a biomaterial thereof and application of the protein. The application solves the technical problem of improving plant disease resistance, reducing plant tillering number, delaying plant flowering time or preparing a plant with delayed flowering time. The application specifically provides a protein and a biomaterial thereof. The protein is any one of the following: A1) a protein with an amino acid sequence shown in sequence 2; A2) a protein with more than 80% identity with the protein shown in A1) and capable of improving plant disease resistance, which is obtained by substitution, deletion and / or addition of amino acid residues of the protein in A1); and A3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1) or A2). Replacing a coding gene of the protein in a related gene in a plant genome can improve plant disease resistance, reduce plant tillering number and delay plant flowering time, and is used for rice variety improvement or breeding.

The application discloses a laccase mutant, and preparation and application thereof, and solves the technical problem of providing an enzyme mutant with higher enzyme activity and a complex formed by the enzyme mutant and bacillus subtilis with higher enzyme stability. The application specifically discloses a protein, which is any one of the following: B1) a protein with an amino acid sequence shown in sequence 3; B2) a protein with more than 80% identity with the protein shown in B1) and with the same function, which is obtained by substitution, deletion and / or addition of amino acid residues of the protein in B1); and B3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of the protein in B1) or B2). The protein can be used alone to degrade zearalenone and aflatoxin B1, or the protein can be mixed with bacillus subtilis to form a complex, so that the stability of the enzyme can be improved, and the complex can be used in industrial production.

The present invention relates to a method for separating and / or isolating exosomes from a sample, the method comprising the steps of i) providing a sample that is known to comprise or suspected of comprising exosomes; ii) contacting a first binding agent with the sample and allowing the binding agent to bind to exosomes, wherein the first binding agent comprises a) cinnamycin and fluoresceine isothiocyanate (FITC), wherein the FITC is covalently linked to cinnamycin, or b) a protein-tag linked to cinnamycin via a peptide linker, wherein the peptide linker comprises one or more proteolytic cleavage sites; iii) separating and / or isolating complexes of the first binding agent and exosomes from the sample. The present invention further relates to a binding agent to be used in the method and to kits comprising the binding agent and instructions for binding of said binding agent to exosomes in a sample.

The application discloses a protein GmMBR4 for regulating plant type and yield, a coding gene thereof and application thereof. The application belongs to the field of plant breeding and particularly relates to the protein GmMBR4 for regulating plant type and yield, the coding gene thereof and application thereof. The protein of the application is any one of the following: A1) a protein with an amino acid sequence shown in SEQ ID No. 2; A2) a protein obtained by substitution, deletion and / or addition of amino acid residues on the protein of A1) and having more than 80% identity with the protein shown in A1) and having the same function; and A3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1) or A2). Experiments prove that the protein GmMBR4 can regulate plant type (especially plant height or / and plant branch number) and yield (pod number per plant or / and grain number per plant), and has important theoretical significance for soybean breeding.

This invention discloses a transcription factor gene PaZFP36 in *Lycoris radiata* that enhances plant cold resistance and yield, and its application. This invention belongs to the field of biotechnology. The protein PaZFP36 is any one of the following: A1) a protein with the amino acid sequence shown in SEQ ID No:2; A2) a protein obtained by substituting and / or deleting and / or adding amino acid residues to the protein of A1), possessing more than 80% identity and the same function as the protein shown in A1); or a fusion protein obtained by attaching a protein tag to the N-terminus and / or C-terminus. Overexpression in wild-type *Lycoris radiata*. PaZFP36 The gene can significantly improve the cold resistance and yield of 'Liqiu' Napier grass, which has important production application value for creating parent plants with excellent cold resistance and high yield characteristics and for breeding cold-resistant and high-yielding varieties.

This disclosure provides the use of tagged proteins in eliminating interference in immunoassays. Depending on the type of protein tag contained in the protein raw materials used in the diagnostic reagent, this disclosure involves adding one or more tagged proteins or recombinant proteins fused together with multiple tagged proteins to the diagnostic reagent. This addresses the problem of interference with test results caused by the presence of tagged protein antibodies or other substances binding to the tagged proteins in the sample, thereby improving the reagent's anti-interference capability.

The application discloses an electron transfer chain subunit with low-temperature activity, a coding gene and application thereof, and relates to the technical fields of genetic engineering and enzyme engineering. The electron transfer chain subunit is any one of the following (1), (2) and (3): (1) a hoNDUFA12 protein, the amino acid sequence of which is shown in SEQ ID NO. 2; (2) a protein with more than 90% identity with the hoNDUFA12 protein and the same biological function as the hoNDUFA12 protein, which is obtained by substituting, deleting and / or adding one or more amino acid residues to the amino acid sequence of the hoNDUFA12 protein; and (3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of (1) or (2). The electron transfer chain subunit can improve the low-temperature resistance of organisms, and thus can be applied to low-temperature trait improvement and industrial production.

This invention discloses a *Stachys pubescens* alcohol dehydrogenase, its encoding gene, and its applications, relating to the field of microbial genetic engineering technology. The alcohol dehydrogenase is any one of (1), (2), and (3): (1) the SvADH1 protein, whose amino acid sequence is shown in SEQ ID NO.3; (2) a protein obtained by substituting, deleting, and / or adding one or more amino acid residues to the amino acid sequence of the SvADH1 protein, resulting in a protein with more than 70% identity to the SvADH1 protein and associated with the redox of cross-linked solanol A; (3) a fusion protein obtained by attaching a protein tag to the N-terminus and / or C-terminus of (1) or (2). This invention has found that overexpression of the encoding gene of this alcohol dehydrogenase in *Stachys pubescens* significantly increases the yield of cross-linked solanol A, thus providing a new direction for the development of microbially derived cross-linked solanol A.

This invention discloses the sorghum mixed-glucan regulatory gene SbMLG1 and its application in improving forage quality. This invention belongs to the field of biotechnology, specifically relating to the sorghum mixed-glucan regulatory gene SbMLG1 and its application in improving forage quality. This invention provides any of the following proteins: A1) a protein with the amino acid sequence shown in SEQ ID No:2; A2) a protein obtained by substituting and / or deleting and / or adding amino acid residues to the protein of A1), possessing more than 75% identity and the same function as the protein shown in A1); A3) a fusion protein obtained by attaching a protein tag to the N-terminus and / or C-terminus of A1) or A2). Obtaining stable and high-quality transgenic sorghum materials through transgenics provides excellent germplasm resources for forage production and new technical support for subsequent germplasm resource innovation, possessing significant theoretical and practical application value.

This invention discloses the wheat heat-resistant protein TaNAC034, its encoding gene, and its applications, addressing the technical problem of regulating plant heat resistance. Specifically, it discloses the application of the protein, a substance regulating the expression of the gene encoding the protein, or a substance regulating the activity or content of the protein in any of the following: A1) application in regulating plant heat resistance and / or application in preparing products that regulate plant heat resistance; A2) application in regulating plant dry weight under heat stress and / or application in preparing products that regulate plant dry weight under heat stress; A3) application in regulating plant fresh weight under heat stress and / or application in preparing products that regulate plant fresh weight under heat stress; the protein is any of the following: B1) a protein with the amino acid sequence shown in Sequence 2; B2) a protein obtained by substituting and / or deleting and / or adding amino acid residues of the protein in B1) that has more than 80% identity and the same function as the protein shown in B1); B3) a fusion protein obtained by attaching a protein tag to the N-terminus and / or C-terminus of B1) or B2). By regulating the expression of the TaNAC034 gene in plants, heat tolerance can be controlled, which can be used in industrial production.

The application discloses an Lrp mutant and application thereof. The application relates to an Lrp mutant and application thereof in the field of biotechnology. The leucine-responsive transcriptional regulator mutant provided by the application is any one of the following: A1) a protein obtained by mutating a histidine at the 107th position of a protein with an amino acid sequence of SEQ ID No: 1 into tyrosine; A2) a protein obtained by substituting, deleting and / or adding amino acid residues of the protein of A1) and having 75% or more identity with the protein shown in A1) and the same function; and A3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1) or A2). The yield of L-valine of the engineering strain is improved through site-directed mutation, and the application has industrial application value.

The application discloses a protein GmLTPP for regulating soybean plant type and improving yield, a coding gene thereof and application. The application belongs to the field of plant breeding and relates to the protein GmLTPP for regulating soybean plant type and improving yield, the coding gene thereof and application. The protein GmLTPP can regulate plant type and yield, and the protein specifically refers to the following: A1) a protein with an amino acid sequence shown in SEQ ID No. 2; A2) a protein obtained by substitution, deletion and / or addition of amino acid residues of the protein of A1) and having more than 80% identity with the protein shown in A1) and the same function; and A3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1) or A2). Experiments prove that knocking out the protein GmLTPP can change the soybean plant type and improve the yield, and has important theoretical significance for soybean breeding.

The application discloses a salt stress regulation gene of rice OsDNAJ and application thereof. The application belongs to the technical field of biotechnology, and particularly relates to a salt stress regulation gene of rice OsDNAJ and application thereof. The protein OsDNAJ is any one of the following proteins: A1) an amino acid sequence shown in SEQ ID No: 3; A2) a protein obtained by substitution, deletion and / or addition of amino acid residues of the protein of A1) and having more than 75% identity with the protein shown in A1) and having the same function; and A3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1) or A2). After CRISPR gene editing knockout of the gene, OsDNAJ after salt stress treatment, osdnaj the knockout mutant presents a salt tolerance phenotype, indicating that OsDNAJ the gene plays an important role in regulating salt tolerance of rice.