173 results about "Protein tag" patented technology

The invention discloses a recombinant XII type collagen as well as a preparation method and application thereof. The amino acid sequence of the recombinant XII type collagen is any one of (A1)-(A3); (A1) is an amino acid sequence as shown in SEQ ID NO: 1; (A2) is an amino acid sequence which is obtained by substitution, deletion or insertion of one or more amino acid residues of the amino acid sequence as shown in SEQ ID NO: 1 and has the same functional protein; and (A3) is an amino acid sequence of a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of the sequence (A1) or (A2). The recombinant XII type collagen provided by the invention has good antioxidant activity, and has a repairing effect on the problems of skin laxity, wrinkle increase and the like caused by degradation of type I collagen fibers of aged skin.

The invention discloses application of a TaLBD30 protein and a coding gene thereof in regulating and controlling a wheat plant type. The invention belongs to the technical field of biology, and particularly relates to application of TaLBD30 protein and a coding gene thereof to regulation and control of wheat plant types. The protein is any one of the following proteins: A1) a protein with an amino acid sequence as shown in SEQ ID No: 1; a2) a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues on the protein of A1), has 80% or more of identity with the protein of A1) and has the same function as the protein of A1); a3) a fusion protein obtained by connecting a protein tag to the N terminal or / and the C terminal of A1) or A2). TaLBD30 is a transcription factor with transcription inhibition activity, and compared with a wild type, the TaLBD30 gene overexpression shows that after TaLBD30 overexpression, the wheat plant height and ear length are remarkably reduced.

The invention discloses application of programmable TnpB nuclease gene editing, and relates to the technical field of gene editing. The TnpB nuclease is any one of (A1), (A2) and (A3): (A1) TnpB nuclease, the amino acid sequence of which is as shown in SEQ ID NO.1; (A2) carrying out substitution, deletion and / or addition of one or more amino acid residues on the amino acid sequence of the TnpB nuclease to obtain a protein which has 80% or more of identity with the TnpB nuclease and has the same biological function with the TnpB nuclease; and (A3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of (A1) or (A2). The TnpB nuclease provided by the invention has cis-cleavage activity and trans-cleavage activity, and has a wide application prospect in the fields of nucleic acid detection and gene editing.

The invention discloses a transport protein of Stemphylium solanol A as well as a coding gene and application thereof, and relates to the technical field of microbial genetic engineering. The transporter is any one of (1), (2) and (3): (1) SvMFS1 protein, the amino acid sequence of which is as shown in SEQ ID NO.3; (2) carrying out substitution, deletion and / or addition of one or more amino acid residues on the amino acid sequence of the SvMFS1 protein to obtain a protein which has more than 90% of identity with the SvMFS1 protein and is related to crosslink solanol A transport; and (3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of (1) or (2). Research finds that overexpression of the coding gene of the transporter in Stemphylium cystosum can significantly improve the yield of interlinked solanol A, thereby providing a new direction for developing microbial-sourced interlinked solanol A.

The invention discloses an electron transport chain subunit hoSDHD with low-temperature activity and application of the electron transport chain subunit hoSDHD, and relates to the technical field of gene engineering and the technical field of enzyme engineering. The electron transport chain subunit provided by the invention is any one of the following items (1), (2) and (3): (1) hoSDHD protein, the amino acid sequence of which is shown as SEQ ID NO.2; (2) carrying out substitution, deletion and / or addition of one or more amino acid residues on the amino acid sequence of the hoSDHD protein to obtain a protein which has more than 90% of identity with the hoSDHD protein and has the same biological function with the hoSDHD protein; and (3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of (1) or (2). The electron transport chain subunit can improve the low-temperature resistance of organisms, so that the electron transport chain subunit is applied to low-temperature character improvement and industrial production.

The invention discloses an electron transport chain subunit acCYTB with low-temperature activity and application of the electron transport chain subunit acCYTB, and relates to the technical field of gene engineering and the technical field of enzyme engineering. The electron transport chain subunit provided by the invention is any one of the following (1), (2) and (3): (1) acCYTB protein, the amino acid sequence of which is as shown in SEQ ID NO.2; (2) carrying out substitution, deletion and / or addition of one or more amino acid residues on the amino acid sequence of the acCYTB protein to obtain a protein which has more than 90% of identity with the acCYTB protein and has the same biological function with the acCYTB protein; and (3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of (1) or (2). The electron transport chain subunit can improve the low-temperature resistance of organisms, so that the electron transport chain subunit is applied to low-temperature character improvement and industrial production.

Disclosed in the present invention is the use of a substance that reduces the activity or content of the protein TST3b, or a substance that inhibits or reduces the expression of a gene encoding the protein TST3b. In particular, the TST3b protein may be a protein of A1), A2) or A3): A1) a protein having the amino acid sequence of SEQ ID No. 2 in the sequence listing; A2) a protein that is obtained by means of the substitution and / or deletion and / or addition of one or more amino acid residues in the protein of A1), and has 90% or more identity to and the same activity as the protein as shown in A1); A3) a fusion protein obtained by means of linking a protein tag at the N-terminus or / and C-terminus of A1) or A2). The TST3b protein and biological material related thereto can be used to regulate the content of soluble sugar in and the single fruit weight of Solanum lycopersicum.

The invention discloses a method for annotating a vesicle cell subset based on monovesicle membrane proteomics, and belongs to the technical field of vesicle cell annotation. The method comprises the following steps: 1, carrying out exosome collection on a plasma sample to obtain a sample; 2, coding and labeling the antibody probe, and adding a protein tag and a labeled DNA sequence into the antibody probe to obtain a labeled antibody probe; 3, preparing a corresponding combined product; 4, adding a labeled antibody probe into the sample to obtain an exosome compound; step 5, capturing an exosome conjugate through CTB; 6, adding the binding product into the exosome conjugate to generate a sequence; 7, constructing a sequence library and sequencing to obtain a sequencing sequence; 8, removing a sequencing sequence with low expression quantity to obtain an exosome expression data matrix, and standardizing the exosome expression data matrix; 9, carrying out clustering analysis to find out an exosome core subgroup; and step 10, determining the source of the exosome core subgroup, and annotating the single vesicle subgroup.

The invention discloses glycosyltransferase in safflower and a related biological material and application thereof. The invention firstly discloses any one of the following proteins: A1) a protein consisting of an amino acid sequence as shown in a sequence 2; a2) a fusion protein obtained by connecting the N end or / and C end of the protein as shown in the sequence 2 with a protein tag. The invention further discloses the protein related biological material and application thereof. The HSYA biosynthesis key glycosyl transferase gene UGT708U5 is obtained from safflower for the first time, it is proved that the protein UGT708U5 coded by the gene can catalyze phloretin to form HSYA precursor compounds, namely phloretin-mono-C-glucoside and phloretin-di-C-glucoside, and the gene plays an important role in biosynthesis and heterologous production of HSYA; the method has important theoretical and practical significance for regulating and producing the quinonechalcone C-glycoside compounds of plants and cultivating high-quality safflower carthamus.

Provided herein are compositions, systems, and methods for delivering an effector protein into a cell. The present disclosure, in some aspects, provide novel proteins delivering an effector protein into a cell. The novel proteins are supernegatively charged proteins derived from highly anionic proteins identified from the proteome (e.g., human proteome). The novel protein tags can be associated (e.g., covalently or nocovalently) with the protein to be delivered to facilitate delivery of the effector protein into a cell.

The invention discloses a protein SeCYP710A. The protein SeCYP710A is a protein shown as A1), A2) or A3): A1) a protein with an amino acid sequence shown as SEQ ID No.2; a2) a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues on the amino acid sequence shown in A1), has the same function, is derived from A1) or has 80% or more of identity with the protein shown in A1) and has the same function; and A3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1) or A2). The invention also discloses a protein SeCYP710A related biological material and application thereof in improving the salt tolerance of plants. The protein SeCYP710A related biological material can be used for regulating and controlling the salt tolerance of the plants.

The invention discloses a Stemphylium alcohol dehydrogenase as well as a coding gene and application thereof, and relates to the technical field of microbial genetic engineering. The alcohol dehydrogenase is any one of (1), (2) and (3): (1) SvADH1 protein, the amino acid sequence of which is as shown in SEQ ID NO.3; (2) carrying out substitution, deletion and / or addition of one or more amino acid residues on the amino acid sequence of the SvADH1 protein to obtain a protein which has more than 70% of identity with the SvADH1 protein and is related to cross-linked solanol A redox; and (3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of (1) or (2). Research finds that overexpression of the coding gene of the alcohol dehydrogenase in Stemphylium cystosum can significantly improve the yield of the interlinked solanol A, so that a new direction is provided for developing the interlinked solanol A from microorganisms.

The invention relates to the technical field of biology, and discloses application of a substance for regulating and controlling Zmmrpa10 protein expression in regulating and controlling high temperature resistance of corn. The protein Zmmrpa10 disclosed by the invention can be specifically a protein of A1), A2) or A3) as follows: A1) a protein of which the amino acid sequence is SEQ ID No.2 in a sequence table; a2) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues of the protein of A1), has 90% or more of identity with the protein of A1) and has the same activity as the protein of A1); a3) a fusion protein obtained by connecting a protein tag to the N terminal or / and the C terminal of A1) or A2). The Zmmrpa10 protein and related biological materials thereof can be used for regulating and controlling the heat resistance of corn.

The invention belongs to the technical field of nano drug delivery and antibody preparation, and particularly relates to a nano self-assembly anti-Abeta antibody as well as a preparation method and application thereof. A first protein tag is connected to a site which does not influence protein self-assembly after a self-assembly nanoparticle protein is exposed to the outer surface and an exogenous sequence is inserted, a second protein tag is connected to the N end of an anti-Abeta antibody, and a polypeptide penetrating through a blood brain barrier is connected to the C end of the anti-Abeta antibody; the first protein tag and the second protein tag form a covalent bond through spontaneous reaction, and the anti-Abeta antibody is directionally loaded on the surface of the self-assembled nanoparticle protein to form the nano self-assembled anti-Abeta antibody, so that the stability of the anti-Abeta antibody is improved, and the reduction of curative effect caused by degradation of the anti-Abeta antibody is effectively avoided; moreover, the polypeptide which is connected to the C end of the anti-Abeta antibody and passes through the blood brain barrier can enable the anti-Abeta antibody to break through the blood brain barrier, so that the permeability of the antibody to the brain and the accurate delivery of the antibody to a target site are enhanced, and the treatment effect of the Alzheimer's disease is enhanced.

The application discloses a kind of cell membrane chromatographic column based on biotin ligase directional covalent fixation and its preparation method and application, belong to chromatography science and technology field. By bonding 2-imino biotin to silica gel, then using biotin ligase specific recognition with Avi-Tag cell membrane fragments, and stable covalent bonding occurs with 2-imino biotin silica gel, so that cell membrane is fixed on the surface of silica gel to obtain Avi-Tag bridged cell membrane chromatographic stationary phase, finally using wet column packing, obtain cell membrane chromatographic column based on biotin ligase directional covalent fixation. The cell membrane chromatographic column can prolong the service life of cell membrane chromatographic column, improve the stability of cell membrane chromatographic column during use, and the short peptide tag (Avi-Tag) used can improve the non-specific adsorption and poor specificity of the cell membrane chromatographic column prepared based on protein tag technology to a certain extent, providing technical support for the wide application of cell membrane chromatographic column.

The invention provides a method for rapidly finding amino acid of a target protein combined with a protein degradation agent. According to the method, a mutant type or a wild type of an exogenous expression target protein is provided with a protein tag, an exogenous expression tagged protein band and an endogenous expression label-free protein band can be separated, and a degradation agent can only degrade one of the bands and cannot degrade endogenous and exogenous target protein bands at the same time, so that the degradation efficiency is improved. If the endogenous band is a wild type target protein, the exogenous band is a mutant type target protein, and if the endogenous band is a mutant type target protein, the exogenous band is a wild type target protein. According to the invention, by using a degradation agent DHL-11 of IMPDH2, the key amino acid of DHL-11 binding IMPDH2 protein is successfully identified.

The invention relates to the technical field of genetic engineering, and discloses a genetically engineered bacterium for producing beta-ionone at high yield and application of the genetically engineered bacterium. A synthetic biological plasmid modularization technology is adopted, coding genes of carotenoid cleavage dioxygenase NtCCD1 in tobacco are excavated, and specific soluble protein tags including a thioredoxin tag, a maltose binding protein tag and a glutathione transferase tag are screened, so that the content of the carotenoid cleavage dioxygenase NtCCD1 in the tobacco is determined. The NtCCD1 is subjected to single-point mutation modification of mutation of 431st-site histidine into tyrosine, lysine or glutamine, and the fermentation enzyme and the gene engineering bacterium which can efficiently catalyze splitting decomposition of beta-carotene in tobacco to produce beta-ionone are constructed. The biosynthesis of the tobacco aroma component beta-ionone is realized, and the method has high efficiency.

The invention discloses a protein with lipopolysaccharide degrading enzyme activity and a gene and application thereof. The invention belongs to the field of gene engineering, and particularly relates to a protein with lipopolysaccharide degrading enzyme activity and a gene and application thereof. The protein disclosed by the invention is any one of the following proteins: 1) an amino acid sequence is SEQ ID No: 9 or SEQ ID No: 5 in a sequence table; 2) a protein which is obtained by substituting and / or deleting and / or adding one or more amino acid residues to the protein in 1) and has the same function; and 3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of 1) or 2). The protein ALP3 with lipopolysaccharide degrading enzyme activity developed by the invention lays a foundation for low-cost production of high-yield novel lipopolysaccharide degrading enzyme, and has important application potential in biodegradation of endotoxin in the aquatic product field.

The invention discloses MlIW92 protein related to wheat powdery mildew resistance as well as a related biological material and application thereof, and belongs to the technical field of biology. The invention aims to solve the technical problem of how to improve the resistance of wheat to powdery mildew. The MlIW92 protein disclosed by the invention is a protein of A1, A2 or A3 as follows: A1 is a protein of which the amino acid sequence is an amino acid sequence shown as SEQ ID No.2 in a sequence table; a2, a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues on the amino acid sequence as shown in SEQ ID No.2 in the sequence table, has 80% or more of identity with the protein as shown in A1, and is related to wheat powdery mildew resistance; and A3, a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1 or A2. The protein disclosed by the invention is derived from wild two-grain wheat, and powdery mildew resistance gene resources of wheat disease resistance breeding work are enriched.

The application discloses GmMBR1 protein and a coding gene and application thereof, and belongs to the technical field of biotechnology, and relates to GmMBR1 protein and a coding gene and application thereof. The protein of the application is any one of the following: A1) a protein with an amino acid sequence shown in SEQ ID No. 3; A2) a protein obtained by substitution, deletion and / or addition of amino acid residues on the protein of A1) and having more than 80% identity with the protein shown in A1) and having the same function; and A3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1) or A2). Experiments prove that the protein GmMBR1 can regulate plant type and yield, and has important theoretical significance for soybean breeding.

The present invention relates to the technical field of biology. Disclosed are recombinant enterobacter and a use thereof in degradation of tyrosine and phenylalanine. According to the present invention, enzyme molecules in nature that have both phenylalanine and tyrosine degradation activities are collected by means of gene search, and the phenylalanine and tyrosine degradation activities of the enzyme molecules are tested in vitro; enzyme molecules having excellent activities are obtained by screening; the selected enzyme molecules are transferred into enterobacter, engineered bacteria highly expressing the enzyme molecules and having the ability to efficiently degrade phenylalanine and tyrosine are further selected, and then protein tags are introduced to improve the degradation activities. Finally, an optimal structure is selected by means of gastrointestinal fluid tolerance experiments, an engineered bacterium having degradation activities far higher than the existing degradation activities is obtained, and the engineered bacterium is expected to be used for preparing a drug for treating phenylketonuria and tyrosinase.

The present invention provides methods and compositions based on a non-naturally occurring nucleic acid construct encoding a fusion protein for quantifying secretion levels in an individual cell, which may comprise a protein sequence comprising a cytoplasmic domain, a transmembrane domain, and a vesicular domain, wherein the vesicular domain may comprise a sequence of protein marks, wherein upon expression of the fusion protein by a cell, the fusion protein localizes to the membrane of a secretory vesicle such that the protein mark is located in the lumen of the secretory vesicle, and wherein the protein mark binds to a cell-impermeable marker;whereby, after the secretion of the contents of the secretory vesicle, the protein tag is exposed to the cell-impermeable marker, the fusion protein is recycled back into the cell, and the individual cell is tagged with the marker in relation to the amount of secretion.

The application discloses a method for improving the parthenocarpy rate and / or yield of tomatoes, comprising improving the parthenocarpy rate and / or yield of tomatoes by inhibiting or reducing the expression amount of a gene encoding a TSP4.2 protein in the genome of tomatoes. The TSP4.2 protein can be specifically the protein of A1), A2) or A3) as follows: A1) a protein with an amino acid sequence of SEQ ID No. 2 in the sequence listing; A2) a protein obtained by substitution, deletion and / or addition of one or more amino acid residues in the protein of A1), the protein having more than 90% identity with the protein shown in A1) and having the same activity; and A3) a fusion protein obtained by connecting a protein tag to the N terminal and / or C terminal of the protein of A1) or A2). The TSP4.2 protein and related biological materials can be used for regulating the parthenocarpy rate of tomatoes.

The invention relates to application of OsWRKY26 protein in regulation and control of cold resistance of rice in a seedling stage, belonging to the technical field of biology. The OsWRKY26 protein is a protein as shown in A1, A2 or A3: A1 is a protein with an amino acid sequence shown in SEQ ID No.1 in a sequence table; a2, a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues on the protein of A1, has 75% or more of identity with the protein shown in A1, and has the activity of regulating and controlling the cold tolerance of rice in the seedling stage; and A3, a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1 or A2.

The present application relates to the technical field of biology, and discloses a rice panicle type and / or plant height related protein, a coding gene and application thereof, the protein is named as OsGNA, which is the protein of A1), A2) or A3) as follows: A1) the amino acid sequence is the protein of SEQ ID No.2 in the sequence listing; A2) the protein of A1) is obtained by substitution, deletion and / or addition of one or more amino acid residues, which has more than 90% identity with the protein shown in A1) and has the activity of regulating rice panicle type and / or plant height; A3) the fusion protein obtained by connecting the protein tag at the N terminal or / and C terminal of A1) or A2). The OsGNA gene experiment proves that overexpression of the OsGNA protein makes the panicle type of the receptor rice increase, and the number of panicle grains increases; interference with the expression of the OsGNA gene makes the panicle type of the rice decrease, the number of panicle grains decreases, and the plant height becomes short.

The application discloses application of Pm61 protein and a coding gene thereof in regulating wheat resistance to powdery mildew, and belongs to the field of genetic engineering, and particularly relates to application of Pm61 protein and a coding gene thereof in regulating wheat resistance to powdery mildew. The protein of the application is any one of the following: A1) a protein with an amino acid sequence as shown in SEQ ID No: 2; A2) a protein obtained by substitution, deletion and / or addition of amino acid residues on the protein of A1), the protein having more than 80% identity with the protein shown in A1) and having the same function; and A3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1) or A2). The Pm61 protein can positively regulate the resistance of wheat to powdery mildew, and can be used for wheat breeding with resistance to powdery mildew.

The present invention discloses a DSF quenching enzyme derived from Acinetobacter tankii and its encoding gene and application. The present invention belongs to the technical field of biotechnology and enzyme application, and specifically relates to a DSF quenching enzyme derived from Acinetobacter tankii and its encoding gene and application. The DSF quenching enzyme FadDD1 recombinant protein is as follows: A1) a protein having an amino acid sequence as shown in sequence 2; A2) a protein having an amino acid sequence as shown in the 1-558th of sequence 4; A3) a protein having more than 80% identity and the same function as the protein shown in A1) or A2) obtained by replacing and / or deleting and / or adding amino acid residues of the protein of A1) or A2); A4) a fusion protein obtained by connecting a protein tag at one end of A1) or A2). After heterologous expression of the encoding gene FadDD1 in Escherichia coli, the disease resistance of the host zebrafish to pathogenic bacteria mediated by the QS signal molecule DSF can be significantly improved.

The invention belongs to the technical field of protein engineering, particularly relates to a protein tag with superoxide dismutase activity and application, and provides a protein tag which comprises polypeptide or protein with superoxide dismutase activity. On the second aspect, a fusion protein is provided and comprises the protein tag described in the first aspect and at least one target polypeptide or protein, the target polypeptide or protein is not superoxide dismutase, and the target polypeptide or protein is not superoxide dismutase only by using the protein tag disclosed by the invention. According to the present invention, the technical effects of target polypeptide or protein expression quantity detection (SOD enzyme activity detection), target polypeptide or protein protection from oxidative damage, target polypeptide or protein stability improvement and target polypeptide or protein solubility improvement can be simultaneously achieved without adding any additional step compared with the fusion protein expression operation in the prior art; the operation is convenient.

The invention belongs to the field of biological analysis and detection, and particularly relates to a method for screening a listeria monocytogenes hemolysin O inhibitor in vitro, application and the inhibitor. According to the method, hemolysin O inhibitor molecules are screened by detecting fluorescence resonance energy transfer (FRET) signal changes in vitro. The specific method comprises the following steps: firstly, fusing a protein tag to a hemolysin O protein through a genetic engineering method, then respectively covalently labeling FRET donor-receptor fluorescent small molecules to the hemolysin O through specific combination of tag protein and a substrate, and according to the characteristic that the cholesterol-dependent listeria monocytogenes hemolysin O is self-assembled in a solution to form nano-pores, carrying out self-assembly on the listeria monocytogenes hemolysin O to form the nano-pores. The pore-forming activity of the hemolysin O in a solution is monitored through the FRET effect between the assembled small fluorescent molecules, the inhibitor can inhibit assembly of the hemolysin O to weaken the FRET effect of the small fluorescent molecules, and then the pore-forming inhibitor of the hemolysin O can be rapidly screened in vitro through FRET. The screening method has the characteristics of simplicity in operation, rapidness and high sensitivity.

The present invention relates to a plant-derived recombinant IL-37a protein and a preparation method and application thereof, and belongs to the field of biomedical technology. In order to solve the problem that the existing expression system cannot achieve high-yield and low-cost production of IL-37a protein, the present invention provides a plant-derived recombinant IL-37a protein and a preparation method and application thereof. The present invention designs an IL-37a mutant monomer recombinant sequence based on the IL-37a protein, and on the basis of the IL-37a mutant monomer recombinant sequence, three protein tags CTB, CPP, TEV and two signal peptides SP 1 and SP 2 Three recombinant IL‑37a protein encoding genes were designed, and plant-derived recombinant IL‑37a protein was produced using plant bioreactor tobacco. 4 mg of total protein could be extracted from 1 gram of tobacco leaves, and the content of the target protein after purification was about 0.2 mg, achieving high-yield and low-cost production of recombinant IL‑37a protein.