93 results about "Protein tag" patented technology

The invention discloses application of a TaLBD30 protein and a coding gene thereof in regulating and controlling a wheat plant type. The invention belongs to the technical field of biology, and particularly relates to application of TaLBD30 protein and a coding gene thereof to regulation and control of wheat plant types. The protein is any one of the following proteins: A1) a protein with an amino acid sequence as shown in SEQ ID No: 1; a2) a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues on the protein of A1), has 80% or more of identity with the protein of A1) and has the same function as the protein of A1); a3) a fusion protein obtained by connecting a protein tag to the N terminal or / and the C terminal of A1) or A2). TaLBD30 is a transcription factor with transcription inhibition activity, and compared with a wild type, the TaLBD30 gene overexpression shows that after TaLBD30 overexpression, the wheat plant height and ear length are remarkably reduced.

The invention discloses a method for annotating a vesicle cell subset based on monovesicle membrane proteomics, and belongs to the technical field of vesicle cell annotation. The method comprises the following steps: 1, carrying out exosome collection on a plasma sample to obtain a sample; 2, coding and labeling the antibody probe, and adding a protein tag and a labeled DNA sequence into the antibody probe to obtain a labeled antibody probe; 3, preparing a corresponding combined product; 4, adding a labeled antibody probe into the sample to obtain an exosome compound; step 5, capturing an exosome conjugate through CTB; 6, adding the binding product into the exosome conjugate to generate a sequence; 7, constructing a sequence library and sequencing to obtain a sequencing sequence; 8, removing a sequencing sequence with low expression quantity to obtain an exosome expression data matrix, and standardizing the exosome expression data matrix; 9, carrying out clustering analysis to find out an exosome core subgroup; and step 10, determining the source of the exosome core subgroup, and annotating the single vesicle subgroup.

The invention relates to the technical field of biology, and discloses application of a substance for regulating and controlling Zmmrpa10 protein expression in regulating and controlling high temperature resistance of corn. The protein Zmmrpa10 disclosed by the invention can be specifically a protein of A1), A2) or A3) as follows: A1) a protein of which the amino acid sequence is SEQ ID No.2 in a sequence table; a2) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues of the protein of A1), has 90% or more of identity with the protein of A1) and has the same activity as the protein of A1); a3) a fusion protein obtained by connecting a protein tag to the N terminal or / and the C terminal of A1) or A2). The Zmmrpa10 protein and related biological materials thereof can be used for regulating and controlling the heat resistance of corn.

The application discloses a kind of cell membrane chromatographic column based on biotin ligase directional covalent fixation and its preparation method and application, belong to chromatography science and technology field. By bonding 2-imino biotin to silica gel, then using biotin ligase specific recognition with Avi-Tag cell membrane fragments, and stable covalent bonding occurs with 2-imino biotin silica gel, so that cell membrane is fixed on the surface of silica gel to obtain Avi-Tag bridged cell membrane chromatographic stationary phase, finally using wet column packing, obtain cell membrane chromatographic column based on biotin ligase directional covalent fixation. The cell membrane chromatographic column can prolong the service life of cell membrane chromatographic column, improve the stability of cell membrane chromatographic column during use, and the short peptide tag (Avi-Tag) used can improve the non-specific adsorption and poor specificity of the cell membrane chromatographic column prepared based on protein tag technology to a certain extent, providing technical support for the wide application of cell membrane chromatographic column.

The invention relates to the technical field of genetic engineering, and discloses a genetically engineered bacterium for producing beta-ionone at high yield and application of the genetically engineered bacterium. A synthetic biological plasmid modularization technology is adopted, coding genes of carotenoid cleavage dioxygenase NtCCD1 in tobacco are excavated, and specific soluble protein tags including a thioredoxin tag, a maltose binding protein tag and a glutathione transferase tag are screened, so that the content of the carotenoid cleavage dioxygenase NtCCD1 in the tobacco is determined. The NtCCD1 is subjected to single-point mutation modification of mutation of 431st-site histidine into tyrosine, lysine or glutamine, and the fermentation enzyme and the gene engineering bacterium which can efficiently catalyze splitting decomposition of beta-carotene in tobacco to produce beta-ionone are constructed. The biosynthesis of the tobacco aroma component beta-ionone is realized, and the method has high efficiency.

The invention discloses a protein with lipopolysaccharide degrading enzyme activity and a gene and application thereof. The invention belongs to the field of gene engineering, and particularly relates to a protein with lipopolysaccharide degrading enzyme activity and a gene and application thereof. The protein disclosed by the invention is any one of the following proteins: 1) an amino acid sequence is SEQ ID No: 9 or SEQ ID No: 5 in a sequence table; 2) a protein which is obtained by substituting and / or deleting and / or adding one or more amino acid residues to the protein in 1) and has the same function; and 3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of 1) or 2). The protein ALP3 with lipopolysaccharide degrading enzyme activity developed by the invention lays a foundation for low-cost production of high-yield novel lipopolysaccharide degrading enzyme, and has important application potential in biodegradation of endotoxin in the aquatic product field.

The invention discloses MlIW92 protein related to wheat powdery mildew resistance as well as a related biological material and application thereof, and belongs to the technical field of biology. The invention aims to solve the technical problem of how to improve the resistance of wheat to powdery mildew. The MlIW92 protein disclosed by the invention is a protein of A1, A2 or A3 as follows: A1 is a protein of which the amino acid sequence is an amino acid sequence shown as SEQ ID No.2 in a sequence table; a2, a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues on the amino acid sequence as shown in SEQ ID No.2 in the sequence table, has 80% or more of identity with the protein as shown in A1, and is related to wheat powdery mildew resistance; and A3, a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1 or A2. The protein disclosed by the invention is derived from wild two-grain wheat, and powdery mildew resistance gene resources of wheat disease resistance breeding work are enriched.

The application discloses GmMBR1 protein and a coding gene and application thereof, and belongs to the technical field of biotechnology, and relates to GmMBR1 protein and a coding gene and application thereof. The protein of the application is any one of the following: A1) a protein with an amino acid sequence shown in SEQ ID No. 3; A2) a protein obtained by substitution, deletion and / or addition of amino acid residues on the protein of A1) and having more than 80% identity with the protein shown in A1) and having the same function; and A3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1) or A2). Experiments prove that the protein GmMBR1 can regulate plant type and yield, and has important theoretical significance for soybean breeding.

The present invention provides methods and compositions based on a non-naturally occurring nucleic acid construct encoding a fusion protein for quantifying secretion levels in an individual cell, which may comprise a protein sequence comprising a cytoplasmic domain, a transmembrane domain, and a vesicular domain, wherein the vesicular domain may comprise a sequence of protein marks, wherein upon expression of the fusion protein by a cell, the fusion protein localizes to the membrane of a secretory vesicle such that the protein mark is located in the lumen of the secretory vesicle, and wherein the protein mark binds to a cell-impermeable marker;whereby, after the secretion of the contents of the secretory vesicle, the protein tag is exposed to the cell-impermeable marker, the fusion protein is recycled back into the cell, and the individual cell is tagged with the marker in relation to the amount of secretion.

The application discloses a method for improving the parthenocarpy rate and / or yield of tomatoes, comprising improving the parthenocarpy rate and / or yield of tomatoes by inhibiting or reducing the expression amount of a gene encoding a TSP4.2 protein in the genome of tomatoes. The TSP4.2 protein can be specifically the protein of A1), A2) or A3) as follows: A1) a protein with an amino acid sequence of SEQ ID No. 2 in the sequence listing; A2) a protein obtained by substitution, deletion and / or addition of one or more amino acid residues in the protein of A1), the protein having more than 90% identity with the protein shown in A1) and having the same activity; and A3) a fusion protein obtained by connecting a protein tag to the N terminal and / or C terminal of the protein of A1) or A2). The TSP4.2 protein and related biological materials can be used for regulating the parthenocarpy rate of tomatoes.

The invention relates to application of OsWRKY26 protein in regulation and control of cold resistance of rice in a seedling stage, belonging to the technical field of biology. The OsWRKY26 protein is a protein as shown in A1, A2 or A3: A1 is a protein with an amino acid sequence shown in SEQ ID No.1 in a sequence table; a2, a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues on the protein of A1, has 75% or more of identity with the protein shown in A1, and has the activity of regulating and controlling the cold tolerance of rice in the seedling stage; and A3, a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1 or A2.

The present application relates to the technical field of biology, and discloses a rice panicle type and / or plant height related protein, a coding gene and application thereof, the protein is named as OsGNA, which is the protein of A1), A2) or A3) as follows: A1) the amino acid sequence is the protein of SEQ ID No.2 in the sequence listing; A2) the protein of A1) is obtained by substitution, deletion and / or addition of one or more amino acid residues, which has more than 90% identity with the protein shown in A1) and has the activity of regulating rice panicle type and / or plant height; A3) the fusion protein obtained by connecting the protein tag at the N terminal or / and C terminal of A1) or A2). The OsGNA gene experiment proves that overexpression of the OsGNA protein makes the panicle type of the receptor rice increase, and the number of panicle grains increases; interference with the expression of the OsGNA gene makes the panicle type of the rice decrease, the number of panicle grains decreases, and the plant height becomes short.

The application discloses application of Pm61 protein and a coding gene thereof in regulating wheat resistance to powdery mildew, and belongs to the field of genetic engineering, and particularly relates to application of Pm61 protein and a coding gene thereof in regulating wheat resistance to powdery mildew. The protein of the application is any one of the following: A1) a protein with an amino acid sequence as shown in SEQ ID No: 2; A2) a protein obtained by substitution, deletion and / or addition of amino acid residues on the protein of A1), the protein having more than 80% identity with the protein shown in A1) and having the same function; and A3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1) or A2). The Pm61 protein can positively regulate the resistance of wheat to powdery mildew, and can be used for wheat breeding with resistance to powdery mildew.

The invention belongs to the technical field of protein engineering, particularly relates to a protein tag with superoxide dismutase activity and application, and provides a protein tag which comprises polypeptide or protein with superoxide dismutase activity. On the second aspect, a fusion protein is provided and comprises the protein tag described in the first aspect and at least one target polypeptide or protein, the target polypeptide or protein is not superoxide dismutase, and the target polypeptide or protein is not superoxide dismutase only by using the protein tag disclosed by the invention. According to the present invention, the technical effects of target polypeptide or protein expression quantity detection (SOD enzyme activity detection), target polypeptide or protein protection from oxidative damage, target polypeptide or protein stability improvement and target polypeptide or protein solubility improvement can be simultaneously achieved without adding any additional step compared with the fusion protein expression operation in the prior art; the operation is convenient.

The application discloses a vine cutting disease resistance related protein IbCEIL1 and related biological materials and application. The application aims to solve how to regulate the vine cutting disease resistance of plants. The IbCEIL1 is any one of the following proteins: A1) a protein with an amino acid sequence shown in sequence 2; A2) a protein obtained by substitution, deletion and / or addition of amino acid residues of the protein in A1), the protein having more than 80% identity with the protein shown in A1) and having the function of regulating the vine cutting disease resistance of plants; and A3) a fusion protein obtained by connecting an N terminal or / and C terminal of A1) or A2) with a protein tag. A substance for inhibiting or reducing or down-regulating IbCEIL1 gene expression can be used for improving the vine cutting disease resistance of sweet potatoes or for breeding sweet potatoes with vine cutting disease resistance.

The application discloses application of a protein and related biomaterials thereof in regulating soybean plant type and improving yield. The application belongs to the technical field of biotechnology, and particularly relates to application of the protein and related biomaterials thereof in regulating soybean plant type and improving yield. The protein of the application is a composition of proteins with amino acid sequences of SEQ ID No. 1 and / or SEQ ID No. 4; or a protein with a function of regulating plant type or / and yield, which is obtained by substitution, deletion and / or addition of amino acid residues on the above-mentioned protein; or a fusion protein obtained by connecting a protein tag to the N terminal and / or C terminal of the above-mentioned protein. By regulating the activity or content of the above-mentioned protein, a soybean variety with changed plant type and improved yield can be obtained.

This invention relates to the field of biotechnology, and discloses the protein IpSTP5, which is related to the oil content of *Vernicia fordii* fruit, and its related biomaterials and applications. Specifically, the IpSTP5 protein can be one of the following: A1), A2), or A3): A1) a protein whose amino acid sequence is SEQ ID No. 2 in the sequence listing; A2) a protein obtained by substituting and / or deleting and / or adding one or more amino acid residues to the protein of A1), possessing more than 90% identity and the same activity as the protein shown in A1); A3) a fusion protein obtained by attaching a protein tag to the N-terminus and / or C-terminus of A1) or A2). The IpSTP5 protein and its related biomaterials can be used to positively regulate the oil content of *Vernicia fordii* fruit.

The invention discloses an application of TaESV in regulating and controlling carbon-nitrogen balance of wheat. The invention belongs to the technical field of biology, and particularly relates to application of TaESV to regulation and control of wheat carbon-nitrogen balance. The protein TaESV is any one of the following proteins: A1) a protein with an amino acid sequence as shown in SEQ ID No: 1; a2) a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues on the protein of A1), has 80% or more of identity with the protein of A1) and has the same function as the protein of A1); and A3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1) or A2). The edited wheat obtained by knocking out the TaESV protein coding gene grows well under a low-nitrogen condition.

The invention discloses an important protein TaGS2D1 for regulating and controlling wheat grain development as well as a related biological material and application thereof, and particularly discloses application of any one of a protein, a substance for up-regulating or enhancing or improving the expression of a coding gene of the protein or a substance for up-regulating or enhancing or improving the activity or content of the protein. A1) application in improving the yield of the wheat plants and / or application in preparing products for improving the yield of the wheat plants; a2) application in shortening the heading time of the wheat plants and / or application in preparing products for shortening the heading time of the wheat plants; the protein is any one of the following proteins: B1) a protein with an amino acid sequence as shown in a sequence 2; b2) a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues of the protein B1), has 80% or more of identity with the protein B1) and has the same function as the protein B1); and B3) a fusion protein obtained by connecting the N terminal or / and the C terminal of B1) or B2) with a protein tag. By overexpressing the protein in the wheat plant, the yield of the wheat plant can be increased or the heading time of the wheat plant can be shortened, and the protein can be used for industrial production.

The invention discloses a method for creating low-cadmium-content rice suitable for being planted in heavily cadmium-polluted soil. The invention provides a method for cultivating rice suitable for being planted in cadmium-contaminated soil. The method comprises the step of improving the expression quantity and / or activity of OsHMA3 protein in receptor rice, the OsHMA3 protein is any one of the following proteins: (A1) a protein with an amino acid sequence of SEQ ID No.1; (A2) a protein which is obtained by substituting and / or deleting and / or adding the amino acid sequence as shown in SEQ ID No.1 and has the same function as that of the amino acid sequence as shown in SEQ ID No.1; (A3) protein which is derived from rice, has 80% or more of identity with the amino acid sequence in (A1) or (A2) and has the same function with the amino acid sequence in (A1) or (A2); (A4) a fusion protein obtained by connecting a protein tag to the N terminal and / or C terminal of any one of the proteins (A1)-(A3); the receptor rice is rice with the deletion of the OsNramp5 gene.

The invention relates to the technical field of biology, and discloses a recombinant N-acetylglucosamine-1-phosphate uridine transferase (GlmU) mutant and a preparation method thereof. The sequence of the recombinase is formed by connecting an N-acetylglucosamine-1-uridine phosphate transferase (GlmU) mutant (L113V-V172I) from Azotobacter chroococcum, an N-terminal histidine tag (His-tag) and a small ubiquitin-like modified protein tag (SUMO-tag) in series. The invention further discloses a preparation method of the recombinase. Compared with a recombinant wild type N-acetylglucosamine-1-uridine phosphate transferase with the same source, the recombinant N-acetylglucosamine-1-uridine phosphate transferase mutant has higher activity which is 1.5 times of that of the recombinant wild type and higher stability, and the residual activity of the mutant is 66.5% after the mutant is placed at room temperature for 5 days, so that the mutant has a good application prospect in the field of N-acetylglucosamine-1-uridine phosphate transferase. And the soluble expression quantity of the recombinant mutant N-acetylglucosamine-1-uridine phosphate transferase is increased by 5 times.

The invention discloses a bifunctional protein tag for improving thermal stability and solubility of recombinant protein, a product based on the bifunctional protein tag and application of the bifunctional protein tag, and belongs to the technical field of protein engineering. The amino acid sequence of the bifunctional tag is shown as SEQ ID NO: 1, the bifunctional tag has the similarity of 70%-95% or above, and the amino acid sequence variation mainly exists but is not limited to a secondary structure position. The bifunctional tag can be used at an N terminal or a C terminal of a target protein, and is suitable for expression and purification of a fusion protein in a prokaryotic expression system. The hydrophobic region of the natural protein is transformed through rational design, so that the technical problems that the existing protein tag is large in molecular weight and easy to shield the active structural domain of the target protein, and the residual non-natural amino acid after the tag is removed possibly destroys the correct folding of the protein are solved; the solubility and the thermal stability of the fusion protein in an escherichia coli expression system are remarkably improved, and the method is suitable for functional expression and industrial application of inclusion body protein.

The invention discloses a mannase mutant as well as a coding gene and application thereof. The invention provides a mannase mutant which is any one of the following proteins: A1) the amino acid sequence of the protein is obtained by mutating the 38th Gln of the amino acid sequence of wild type mannase as shown in a sequence 3 into Lys, mutating the 224th Asp into Ala and mutating the 272th Ala into Thr, and the amino acid sequence of the protein is obtained by mutating the 38th Gln of the amino acid sequence of wild type mannase as shown in the sequence 3 into Lys, mutating the 224th Asp of the amino acid sequence of wild type mannase as shown in the sequence 3 into Thr; a2) a fusion protein obtained by connecting the N end or / and C end of the protein shown in A1) with a protein tag. The mannase mutant provided by the invention is derived from aspergillus niger, has good enzymatic properties and hydrolysis characteristics, can efficiently hydrolyze guar bean slices under a natural pH value condition to generate mannan oligosaccharide, and has great application potential in the field of food.

The invention discloses a cell strain capable of stably expressing a multi-protein tag as well as a construction method and application of the cell strain, and belongs to the technical field of biology. The cell strain is transfected by a lentiviral vector, a fusion expression vector containing P2A self-cleavage polypeptide and a plurality of protein tag coding sequences is integrated into a host cell, and a stable expression cell strain and a fusion protein expressing a plurality of protein tags including green fluorescent protein are obtained through antibiotic screening. The cell strain can be used for antibody specificity verification, protein interaction research and multi-label system standardization experiment platforms, and has the advantages of stable expression, simple operation, reliable result and the like.

The invention discloses a bacteriostasis-related protein as well as a biological material and application thereof. The technical problem to be solved by the invention is how to inhibit bacteria. The invention specifically discloses application of the G enzyme. The application is any one of the following items: A1) application in preparation of a product for promoting wound healing; a2) in preparation of a product for promoting healing of infectious wounds; a3) in preparation of a product for promoting and / or assisting in promoting wound healing; a4) in preparation of a product for enhancing the antibacterial and / or bactericidal ability of antibiotics; a5) application in preparation of a product for enhancing the ability of antibiotics to treat or / and prevent eye infection; a6) in improving the cell activity and / or preparing a product for improving the cell activity. The G enzyme is any one of the following items: B1) the amino acid sequence is a protein of a sequence 1 or 31-442 sites of the sequence 1; b2) a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues of the protein B1), has 80% or more of identity with the protein B1) and has the same activity with the protein B1); b3), a fusion protein obtained by connecting the N terminal or / and the C terminal of B1) or B2) with a protein tag. The G enzyme disclosed by the invention has an obvious antibacterial effect when being used for gram-negative bacteria, and can be used for industrial production.

The present disclosure provides a fusion protein, a conjugate containing the same, a kit and use, belonging to the technical field of immunodiagnosis. The fusion protein of the present disclosure comprises a protein tag and a protein part, the fusion protein containing the protein tag has improved activity or specificity, and the fusion protein is used for immunodetection, which can improve the sensitivity or specificity of immunodetection.

The TnpB nuclease is any one of (A1), (A2), and (A3): (A1) the TnpB nuclease having the amino acid sequence shown in SEQ ID NO: 1; (A2) a protein having more than 80% identity with the TnpB nuclease and having an identical biological function as the TnpB nuclease, where the protein is obtained by substituting, deleting, and / or adding one or more amino acid residues to the amino acid sequence of the TnpB nuclease; and (A3) a fusion protein obtained by fusing a protein tag to an N-terminus and / or a C-terminus of (A1) or (A2). The TnpB nuclease possesses both cis-cleavage activity and trans-cleavage activity and has broad application prospects in the fields of nucleic acid detection and gene editing.

The application discloses a plant type and yield related GmMBR2 protein and a coding gene and application thereof, and belongs to the field of plant breeding, and relates to a plant type and yield related GmMBR2 protein and a coding gene and application thereof. The protein of the application is any one of the following: A1) a protein with an amino acid sequence shown in SEQ ID No. 1; A2) a protein obtained by substitution, deletion and / or addition of amino acid residues on the protein of A1) and having more than 80% identity with the protein shown in A1) and having the same function; and A3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1) or A2). Experiments prove that the protein GmMBR2 can regulate plant type and yield, and has important theoretical significance for soybean breeding.

The invention relates to the technical field of biology, and discloses a ZmMYB69 protein related to corn root configuration and heat resistance as well as a related biological material and application of the ZmMYB69 protein. The ZmMYB69 protein can be specifically a protein of A1), A2) or A3) as follows: A1) a protein of which the amino acid sequence is SEQ ID No.2 in a sequence table; a2) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues of the protein of A1), has 90% or more of identity with the protein of A1) and has the same activity as the protein of A1); and A3) a fusion protein obtained by connecting a protein tag to the N terminal or / and the C terminal of A1) or A2). The ZmMYB69 protein and related biological materials thereof can be used for regulating and controlling the root architecture and heat resistance of corn.

The application discloses a cadmium tolerance related protein IbCSase of sweet potato and an encoding gene and application thereof, and belongs to the technical field of biotechnology, and particularly relates to the cadmium tolerance related protein IbCSase of sweet potato and the encoding gene and application thereof. The protein IbCSase is any one of the following: A1) a protein with an amino acid sequence as shown in SEQ ID No. 1; A2) a protein with more than 80% identity with the protein shown in A1) and the same function, which is obtained by substitution, deletion and / or addition of amino acid residues of the protein of A1); and A3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1) or A2). By introducing the IbCSase encoding gene into wild type sweet potato, the tolerance of the plant to heavy metal cadmium can be improved, the IbCSase encoding gene can be applied to sweet potato breeding, and has wide application space and market prospect in the agricultural field.