9 results about "Erwinia" patented technology

The present invention relates to an endophyte strain isolated from a plant of the Poaceae family, wherein said endophyte is a strain of Erwinia gerundensis which provides bioprotection and / or biofertilizer phenotypes to plants into which it is inoculated. The present invention also discloses plants infected with the endophyte and related methods.

The present application provides a kind of compound biocontrol agent and its preparation method and application technology, and it is composed of 1000ml of fermentation mixed liquor of multiple strains of biocontrol bacteria mixed in equal volume ratio and 0.5%-1.5% stabilizer with complementary mechanism and target.The fermentation concentrated solution of multiple strains of bacteria is prepared in fermentation medium to obtain the present application, which is used for preventing and controlling fusarium, pythium, phytophthora, rhizoctonia solani, ralstonia solanacearum, erwinia carotovora, streptomyces scabiei, xanthomonas campestris and other plant pathogenic bacteria and nematodes, which can inhabit in soil for a long time and spread through soil, and one or several of them can cause plant soil-borne diseases.The preparation process of the present application is not affected by too many chemical and physical factors, the activity of live bacteria and metabolites is high, the average control effect is 78%-85%, the application effect is good;The production process is simple, energy and auxiliary materials are saved, and the production cost is low.

The application discloses a pathogenic protein of a soft rot pathogen of Pleurotus eryngii and an encoding gene thereof. The application provides a protein obtained from a Beijing E. coli, which is named as protein 1408 and is a protein shown in sequence 1 in a sequence list. A DNA molecule encoding the protein 1408 is also called as gene 1408. The application also protects the application of the protein 1408, which is as follows: regulating the pathogenicity of the Beijing E. coli; regulating the polysaccharide content of the Beijing E. coli. The inventors of the application first report the soft rot of the Pleurotus eryngii in Beijing and find the Beijing E. coli causing the pathogenicity. After the gene 1408 in the Beijing E. coli is knocked out, the content of the exocellular polysaccharide is reduced and the pathogenicity is reduced. The application has great application value for the pathogenic mechanism research of the Beijing E. coli and the prevention and control of the soft rot.

The invention discloses a pinellia tuber soft rot fungus infection dynamic detection method, which comprises: applying a pH indicator to a to-be-detected surface of a plant tissue sample, and carrying out timing observation after the application; the color change range of the pH indicator at least covers pH 5.0-pH 7.0. The invention provides a dynamic monitoring system combining chromogenic reaction and pH value change, the method can be used for dynamic detection of soft rot fungus infection of pinellia tuber tubers, the method is simple and convenient in detection process operation, does not need complex equipment, is suitable for laboratory environment and can also be used for rapid field detection, the detection efficiency is improved, and the detection scene is expanded.

The application discloses application of Streptomyces sp. D67 as a bacteriostatic agent. A new actinomycete Streptomyces sp. D67 is obtained by separation, screening and identification from Kumutage desert soil in Xinjiang, and the bacterium has significant bacteriostatic properties, and has good bacteriostatic activity on Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Erwinia. The bacteriostatic agent of Streptomyces sp. D67 is diluted to 5 times the volume, and the inhibition rate of the bacteriostatic agent on the growth of Chromobacterium violaceum CV026 reaches 85.6%; the bacteriostatic agent is diluted to 2 times the volume, and the inhibition rate of the bacteriostatic agent on the growth of Pseudomonas aeruginosa reaches 84%; the bacteriostatic agent is diluted to 5 times the volume, and the inhibition rate of the bacteriostatic agent on the growth of Erwinia reaches 74.3%; and it is shown that the Streptomyces sp. D67 provided by the application as the bacteriostatic agent has good development and application prospects in the fields of biological pesticides, microbial preparations and other antibacterial related fields.

A method for enhancing the degradation performance of lignin-degrading bacteria Erwinia sp. QL-Z3 and a culture medium for culturing the bacteria. The rate of degradation of an Erwinia sp. QL-Z3 strain to lignin is optimized from 14.23% before optimization to 25.01%. Under the conditions that the initial pH value of the culture medium is 8, the nitrogen source is NH4NO3, and the addition amount of lignin is 3 g / L, the activity of an LiP enzyme can be optimized to 371.00 U / L, which is 3.53 times that before optimization. When the initial pH value of the culture medium is 9.5, the nitrogen source is NH4NO3, and the concentration of lignin is 2.5 g / L, the activity of MnP and Lac enzymes can be optimized to 839.50 U / L and 219.00 U / L, respectively, which are 3.18 and 2.84 times that before optimization.

The invention discloses a microbial bactericide and application thereof. The microbial bactericide comprises the following components: a functional microbial complex microbial inoculant, a targeting bacteriophage composition, a pH-responsive nanogel carrier and an auxiliary agent, wherein the functional microorganism complex microbial inoculant comprises a bacterial strain which is separated from deep-sea sediments and screened by a plate confrontation method, and the diameter of an inhibition zone of the bacterial strain on at least one target pathogenic bacterium is greater than or equal to 15mm; the targeted bacteriophage composition comprises at least two lytic bacteriophages, and the lytic bacteriophages are used for respectively and specifically splitting different types of pathogenic bacteria including ralstonia solanacearum, pseudomonas syringae and Erwinia; four mechanisms of competitive occupation, antibacterial substance inhibition, bacteriophage lysis and induction system resistance are integrated, and the limitation of a single action mode is broken through.

This invention discloses *Amycolatopsis* sp., its culture, metabolites, bacterial agents, and applications. The *Amycolatopsis* sp. provided by this invention has strain number TRM77291 and accession number CCTCC M 2024327 at the China Center for Type Culture Collection. This strain exhibits antibacterial activity against Erwinia, Salmonella, *Escherichia coli*, and *Pseudomonas aeruginosa*. This invention is of significant importance for the control of Gram-negative bacterial infections and the research of novel actinomycete-derived drugs.