134 results about "Flavobacterium" patented technology

4-(Indol-3-ylmethyl)-4-hydroxy-2-oxoglutarate, which is useful as an intermediate in the synthesis of monatin, may be synthesized from indole pyruvic acid and pyruvic acid (and/or oxaloacetic acid) by using a novel aldolase derived from the genus Pseudomonas, Erwinia, Flavobacterium, or Xanthomonas.

The invention relates to a combined restoring method of polycyclic aromatic hydrocarbon contaminated soil, comprising the following restoring steps of: planting lucerne as perennial leguminous plants in the polycyclic aromatic hydrocarbon contaminated soil; inoculating a mixed bacterial inoculum comprising arbuscular mycorrhizal fungi, bacillus and flavobacterium; and meanwhile, adding rhamnolipid as a biosurfactant. By rhizosphere effect generated by plant root exudates, the number and the metabolic activity of soil microorganisms are increased; and by inoculating the arbuscular mycorrhizal fungi, the plant growth is promoted, and the root exudates are increased, thereby increasing the root accumulation of organic pollutants and the number of root soil microorganisms. The inoculated high-efficiency polycyclic aromatic hydrocarbon degrading bacteria use plant root secretions as a carbon source and an energy source and can quickly grow and propagate. The polycyclic aromatic hydrocarbon is stripped from soil grains by the biosurfactant, and the bioavailability and the degradability of the organic pollutants are increased.

The invention relates to a bacterial strain used for short-cut denitrification for nitrogen removal and its application. The bacterial strain used for short-cut denitrification for nitrogen removal is flavobacterium mizutaii FDN-2, which is preserved in China general microbiological culture collection center on March 11, 2012 and has a preservation number of CGMCC NO.3659. The bacterial strain can directly utilize nitrite nitrogen as a substrate to complete a short-cut denitrification process. When treating ammonia-containing wastewater with the flavobacterium mizutaii FDN-2 of the invention,the process is simple, and the flavobacterium mizutaii FDN-2 can tolerate a high concentration organic carbon source. When the flavobacterium mizutaii FDN-2 in the invention is put into use, a systemcan be rapidly initiated, so that the flavobacterium mizutaii FDN-2 boasts very broad application prospects in various nitrogen removal processes of wastewater.

The present invention discloses a short-cut nitrification-denitrification microbial agent. The microbial agent contains Arthrobacter creatinolyticus FDN 1, Flavobacterium mizutaii FDN 2, Paracoccus denitrificars DN 3 and Methylobacterium phyllosphaerae SDN3. The preservation register numbers of the four strains respectively are CGMCC No.3657, CGMCC No.3659, CGMCC No.3658 and CGMCC No.3660. With the microbial agent, the ammonia nitrogen removing, the total nitrogen removing and the CODcr removing in the same reactor can be achieved, the wastewater treatment effect is good, and the microbial agent is especially suitable for the purification treatment of the wastewater containing the ammonia nitrogen so as to really achieve the short-cut nitrification-denitrification or simultaneous nitrification and denitrification.

The invention discloses a vitamin K2 and preparation process thereof. According to the process, 1-hydroxyl-2-naphthoic acid resistant flavobacterium strain HNA12-D is fermented and cultured, and vitamin K2 efficiently metabolizes in the strain through the batch feeding of glycerin serving as the carbon source, peptone serving as the nitrogen source and precursor substances, and is extracted by using organic solvents and separated by macroporous resin, and the yield and purity of the product are improved. The total output of the vitamin K2 crude product is 200-600 mg/L after the flavobacterium strain HNA12-D of the invention is fermented and cultured for 120-144 hours, and the product purity reaches 80-99 percent after absorption chromatography; compared with the prior art, the yield output and the product purity are greatly improved, and the product can be used in industrial production.

The invention discloses a preparation method of a composite microbial inoculant for degrading aflatoxin B1 and a composite microbial inoculant prepared by the method. The method comprises the following steps: respectively culturing pseudomonad, Flavobacterium, Rhodococcus, Stenotrophomonas and Bacillus to obtain a pseudomonad solution, a Flavobacterium solution, a Rhodococcus solution, a Stenotrophomonas solution and a Bacillus solution; mixing the pseudomonad solution, Flavobacterium solution, Rhodococcus solution, Stenotrophomonas solution and Bacillus solution to obtain a composite bacterium solution; and putting the composite bacterium solution into a composite bacterium culture medium, and carrying out mixed culture fermentation to obtain the composite microbial inoculant for degrading aflatoxin B1. The composite microbial inoculant can efficiently degrade aflatoxin B1, and has the advantages of low cost and no secondary pollution.

The invention relates to a culture method of biological cleaning strains in low-concentration volatile mixed organic waste gas. The method comprises the following steps: a), culturing a first strain; b), carrying out the combined inoculation of the first strain and a second strain and using tap water to prepare the sludge concentration of the mixed strains; and c), using mixed organic matter in the waste gas as a substrate to be cast into an insition culture medium so as to culture and train insitions. The first strain is prepared by culturing and training from activated mud taken from a printing and dyeing sewage treatment plant and is a mixed strain of flavobacterium and Bacillus cereus, and the second strain is prepared by collecting activated mud collected from a secondary sedimentation tank of a petrochemical sewage treatment plant and is a mixed strain comprising the flavobacterium, the Bacillus cereus, xylose oxidase Achromobacter and Pseudomonas stutzeri. The method facilitates the balanced growth of various cultured microorganisms and can simultaneously decompound various target configuration compounds at high efficiency.

The invention discloses microbial fertilizer and a preparation method thereof. The microbial fertilizer is fermentation liquor with hydrogen-oxidizing bacterium content ranging from 1X108/mL to 1X1010/mL or mixture of hydrogen-oxidizing bacterium liquid and sterile grass carbon, the content of hydrogen-oxidizing bacteria in the mixture ranges from 2X108/g to 2X1010/g, moisture of the mixture ranges from 20wt% to 40wt%, and the hydrogen-oxidizing bacteria include one type or a plurality of types of a flavobacterium, a pseudomonas, alcaligenes and burkholderia. The invention further provides a preparation method of the microbial fertilizer. The microbial fertilizer realizes a fine application effect for plants, and is free of environmental pollution, low in consumption and easy in popularization, the preparation process of the microbial fertilizer is simple, the production cycle is short, and cost is low.

The invention provides a microbial fertilizer for reducing residues of pesticides and heavy metals and a preparation method of the microbial fertilizer. The fertilizer is prepared from the raw materials by weight: 50kg of carboxymethyl starch, 80kg of flavobacterium oryzae fermentation liquid, 100kg of aspergillus glaucus fermentation liquid, 100kg of bacillus subtilis fermentation liquid, 3kg of chitosan, 2kg of nucleotide, 20kg of potassium nitrate, 20kg of dipotassium hydrogen phosphate, 50kg of potassium humate and 1500kg of water. The microbial fertilizer provided by the invention is simple in preparation method and capable of remarkably reducing the residues of heavy metals and pesticides in agricultural products and improving the qualities of the agricultural products.

The invention discloses a sludge leavening agent. The sludge leavening agent is prepared from the following raw materials in parts by weight: 15-25 parts of bacillus subtilis, 10-20 parts of bacillus stearothermophilus, 8-15 parts of lactic acid bacteria, 5-10 parts of silicate bacteria, 4-10 parts of nocardia, 9-16 parts of a zotobacter chroococcum, 1-6 parts of saccharomyces cerevisiae, 1-5 parts of flavobacterium oryzae, 2-3 parts of photosynthetic bacteria, 1-3 parts of streptomyces microflavus, 6-13 parts of cellulase, 5-12 parts of amylase and 5-6 parts of xylanase. The sludge leavening agent provided by the invention is environmentally friendly, pollution-free, low in operation cost, small in floor space, and capable of effectively killing pathogenic bacteria, the parasites and the like in the sludge, degrading toxic and harmful substances, accelerating sludge deodorization and dehydration and decomposing nutrition ingredients which are difficult to absorb by crops.

The invention discloses a method for producing a seedling raising substrate by using cotton straw and a substrate prepared with the same. The method comprises the following steps of: smashing cotton straw into sections of 10-50 millimeters; adding water till the water content is 60-80 percent according to mass percentage; adding 2-3kg of urea, 0.5-1kg of calcium superphosphate, 1-2kg of gypsum and 50-100g of fermentation inoculum into each cubic meter of substances obtained in the last step according to the volume ratio; mixing uniformly; and sealing, piling and fermenting at the air temperature of 15-40 DEG C, wherein the adopted compound fermentation inoculum is prepared by mixing pseudomonas aeruginosa, streptomyces albus and flavobacterium in the ratio of 2:3:1, and can be used by fermenting, airing in a scattered way and drying. In the method, a main raw material is derived from a large amount of waste cotton straw generated during the production of cotton, the raw material source is rich, a preparation process is simple, the aim of utilizing resources is fulfilled, and resource waste of cotton straw caused by direct smashing and returning to field can be reduced; and the method has a wide application value.

The invention provides a biological iron-removing manganese-removing functional bacterium, which relates to a microorganism. The invention solves the problems that the present biological oxidation manganese removing engineering flora is established by natural selection, the biochemical capability is weak, the manganese removing effect is poor; the establishment of the water treatment removing manganese biologic filter pond needs a long time for cultivation, domestication and startup, and the restoring period after the engineering flora is damaged is long. The biological iron-removing manganese-removing functional bacterium MSB-4(Chryseobacterium sp.) belongs to flavobacterium, is preserved in the General Microorganism Center of China Commission of Culture Collection Management, the storage date is 30 Octomber 2007, the storage number is CGMCC No.2239; strain MSB-4 is aerobic strain, the length is 1.5-2 micrometers, the width is 0.8-1.1 micrometers, the strain is short rod-shaped cell with two blunt ends; brown yellow colony is formed on the substrate, the colony is non-transparent and round; the strain MSB-4 has metachromatic granules and lipoid granules, has no brood cell; N, N, N<1>, N<1>-tetramethyl-para-phenylene diamine stain is positive. The biological iron-removing manganese-removing functional bacterium MSB-4 of the invention has the capability for removing the Mn<2+>, <Fe2+> in the water, Mn<2+> removing rate can reach up to 94.44 percent, and the Fe<2+> removing rate can reach up to 90.13 percent.

Recalcitrant chemical oxygen demand (COD) of a liquid is reduced in a water treatment system. The method includes pretreating the liquid in a pretreatment unit to remove indigenous bacteria or microbes to a population level below which the indigenous organisms can interfere with the screened and externally introduced microorganisms. The liquid is then provided to a reactor that has a filter bed formed with a carrier material. Special microbes are screened and used to colonize the carrier material to remove recalcitrant COD. A biofilm is cultured on the surface of the carrier material to immobilize the screened microbes in the reactor. The method further includes percolating the liquid from the pretreatment unit through the filter bed colonized with the screened microbes to degrade at least part of the recalcitrant COD under aerobic conditions. In one embodiment, the filter is formed with biological granular activated carbon (GAC) as the carrier material and the screened microbes comprise at least one microbial species selected from the group consisting of Bacillus, Comamonas, Arthrobacter, Micrococcus, Pseudomonas, Pediococcus, Achromobacter, Flavobacterium, Mycobacterium, Rhodanobacter, Stenotrophomonas and Yeast.

The invention discloses an ecological restoration microbial inoculum capable of inhibiting mildews and degrading mycotoxin. The ecological restoration microbial inoculum consists of bacillus subtilis, rhodopseudomonas palustris, enterococcus faecalis, wautersiellfalsenii and candida utilis which are mixed together. A preparation method of the ecological restoration microbial inoculum comprises the following steps: inoculating strains to corresponding sterilized culture medium for fermentation; performing three-stage amplification culture; and finally, adsorbing and solidifying on straw charcoal to prepare a solid microbial inoculum. The ecological restoration microbial inoculum disclosed by the invention has the advantages of being high in density and activity and excellent in characteristic. The bacillus subtilis, the rhodopseudomonas palustris, the enterococcus faecalis and the newly separated wautersiellfalsenii have an effect of inhibiting mildews and degrading mycotoxin, and particularly by cooperation with the candida utilis, the effect of degrading mycotoxin is obviously increased. The ecological restoration microbial inoculum can be used as a feed additive to solve the problem of mildew and mycotoxin pollution in the feed industry, and can also be used as a soil ecological restoration agent to solve the problem of the spreading of a continuous cropping disease in plantation of ginseng and notoginseng.

An effect of reducing sludge amount in a multiple-stage activated sludge method utilizing the predation action of animalcules is stabilized. Organic wastewater is introduced into a first biological treatment tank 1 equipped with aeration means and 70% or more of, preferably 80% or more of, further more preferably 90% or more of the organic components (soluble BOD) is oxidatively decomposed by a common bacterium present in raw water such as Alcaligenes, Pseudomonas, Bacillus, Aerobacter, or Flavobacterium. The treated water in the first biological treatment tank 1 is introduced into a second biological treatment tank 2 and the reduction of the amount of excess sludge is conducted by the oxidative decomposition of remaining organic components, the autolysis of dispersive bacteria and the predation by animalcules. In the second biological treatment tank 2, a fluid bed in which a support C is added so as to increase the holding capacity of the animalcules in the tank is used.

The invention provides a microbial agent. The microbial agent is prepared from the following strain fermentation liquor in parts by weight: 10-50 parts of flavobacterium, 30-60 parts of nitrogen-fixing bacteria and 10-30 parts of zoogloea. The microbial composite inoculant has the advantages that the microbial composite inoculant can replace pesticides to prevent and control soil-borne diseases inthe dry rice growth process and reduce the chemical residues in grains, the toughness and strength of stalk bases can be improved, and the lodging resistance of rice is improved; root system development can be stimulated, root system development is promoted, and root system vitality and ground gripping capacity are improved; automatic nitrogen fixation can be achieved, continuous nitrogen is provided for the whole rice growth period, and abuse of chemical fertilizer is avoided; the yield of drought (water) rice can be increased; and finally, the maturation of dry rice can be advanced by abouttwo weeks, so that the growth period is shortened, and rice enters the market in advance.

The invention discloses a composite microbial preparation for treating watercourse organic matter. The composite microbial preparation is prepared from the following ingredients in percentage by weight: 10 to 20% of pseudomonas, 10 to 15% of vibrio, 10 to 15% of flavobacterium, 10 to 15% of pure nitrite coccus, 10 to 15% of thiobacillus thiooxidant, 10 to 20% of bacillus subtilis, 5 to 15% of bacillus licheniformis, 5 to 10% of aneurinibacillus aneurinilyticus, 5 to 10% of saccharomycetes, 5 to 10% of micrococcus denitrificans and 5 to 10% of actinomycetes. Compared with the prior art, the composite microbial preparation disclosed by the invention can rapidly decompose organic wastes, and effectively inhibits growth of harmful microorganisms; when the composite microbial preparation is put into polluted water, by utilizing metabolic activity of the microorganisms, concentration of pollutants in the water is reduced, the problems of blackening and smelliness of the water can be obviously improved, ammonia nitrogen content and total phosphorus content in the water are reduced, harmful substances such as hydrogen sulfide are decomposed, the water environment is improved, and transparency of the water is obviously improved; the composite microbial preparation has the advantages of simplicity for preparation, low cost and application to watercourse treatment, and has great economic and environmental-friendly significance for watercourse treatment.

The invention relates to flavobacterium and application thereof. The preservation number of the flavobacterium is CGMCC No.6932. The flavobacterium can be used for degrading fucoidan. The flavobacterium with fucoidan enzyme production capacity has high activity in strain enzyme production capacity up to 180 U/ml, and has excellent market application foreground.

The invention belongs to the field of food enterprise wastewater treatment, particularly relates to a bean product production wastewater treating agent and further relates to a bean product production wastewater treating method. The bean product production wastewater treating agent is prepared from a physical water purifying agent, a compound microbial agent and an enzymic preparation, wherein the physical water purifying agent is prepared from, by weight, 1-5 parts of activated carbon, 1-5 parts of organic modified zeolite and 1-5 parts of polyacrylamide, the compound microbial agent is prepared from, by weight, 0.02-0.1 part of flavobacterium powder, 0.05-0.2 part of rhodococcus powder, 0.05-0.2 part of bacillus licheniformis powder and 0.04-0.2 part of thiobacillus powder, and the enzymic preparation is prepared from, by weight, 0.05-0.1 part of cellulose, 0.04-0.09 part of pectinase and 0.01-0.06 part of bromelain. By means of the wastewater treating agent and the wastewater treating method, organic matter which is complex, difficult to degrade and large in particle is hydrolyzed into simple organic matter easy to degrade, the SS content in wastewater is greatly reduced, the BOD and COD removal rate of the treated wastewater is high, and the wastewater reaches the regulated emission standard.

This invention describes a process for preparing a microbe carrier prepared by two times freezing-defreezing of flavorbacteria, comprising the steps of: adding appropriate amounts of activated carbon, zeolite powders and Tween 80 into the PVA- NaíñAlg gel; uniformly mixing the gel and the flavorbacteria seed liquid, and then pouring the mixed liquid into a culture plate that is covered with two layers of non-woven fabrics at 35-48 deg.C; freezing the culture plate in a refrigerator at 10-15 deg.C for 15-18h, defreezing, and repeating the procedure once again; solidifying the mixed liquid with the solification agent, immersing in sterilized water for a time, and then cutting for enrichment culture. The carrier in this invention has good elasticity, diffusion and mechanical properties. The flavorbacteria immobilized have good activities without any leakage.

Safe and effective live vaccines against Flavobacterium columnare of fish were created through the induction of rifampicin resistance in a native Flavobacterium columnare isolate; these including rifampicin-resistant mutants NRRL B-30303 and B-30304. Single immersion exposure of fish stimulated acquired immunity against virulent F. columnare infection.

The invention discloses an application of lactobacillus paracasei L9 to prevention or treatment oral disease and regulation of oral cavity flora. L9 can be used for preparing a medical composition anda food for preventing or treating dental caries. Research proves that L9 can notably relieve generation degree of occlusal surface E-level dental caries, after the LP is taken, the structure of the oral cavity flora is changed notably, particularly actinobacteria is notably increased, the actinobacteria of L9 group is obviously reduced, klebsiella, aerococcus, Gemella, corynebacterium and pseudomonas aeruginosa on the classification level are notably reduced, and the oral disease risk is reduced. When L9 is taken, the saliva species richness is notably reduced, the relative abundance of oraldisease relevant fungi of streptococcus salivarius, actinomycetaceae, peptostreptococcus and the like is notably reduced, and the L9 can have relieving effects on periodonitis diseases. The dental plaque flora species diversity is notably reduced, the relative abundance of leptothrix, fusobacteriaceaeand flavobacterium is notably reduced, and the L9 can have relieving effects on oral diseases of periodontal disease, halitosis, dental caries and the like.

The invention discloses an aflatoxin-removed feed prepared from kitchen waste and a production technology of the aflatoxin-removed feed. The aflatoxin-removed feed is prepared from the following raw materials in percentage by mass: 40-60% of kitchen waste, 30-45% of wheat bran, 4-8% of citric acid, 3-6% of tea polyphenols, 0.1-0.3% of trace elements, 8-12% of a mildew removing agent, and 10-15% ofmildew removing bacteria, wherein the mildew removing agent comprises the following components in parts by weight: 6-12 parts of glucomannan, 11-15 parts of modified attapulgite, 12-16 parts of sorbic acid, 8-14 parts of sodium propionate, and 13-15 parts of quercetin; and the mildew removing bacteria are prepared by mixing bacillus subtilis with lactobacillus casei and orange flavobacterium in the weight ratio of the bacillus subtilis to the lactobacillus casei to the orange flavobacterium being (1.5-2.5) to (2-3) to (1.8-2.6). According to the aflatoxin-removed feed, the growth and the propagation of mould can be effectively restrained, the metabolic pathway generating AFB1 can be restrained, the removing rate of AFB1 can achieve 91.7%, the removing rate of AFB2 can achieve 83.5%, the removing rate of AFG1 can achieve 61.8%, the removing rate of AFG2 can achieve 86.2%, the content of aflatoxins in the feed can be obviously reduced, and the safety and the feasibility for treating thekitchen waste for the feed can be improved.

The invention discloses a method for rapid determination of Flavobacterium farinofermentans toxin A in a food poisoning sample, and belongs to the technical field of food safety biotoxin detection. The method mainly comprises the extraction and condensation of Flavobacterium farinofermentans toxin A in food. Tandem mass spectrometry and ESI source are used to determine a molecular ion peak of Flavobacterium farinofermentans toxin A (M-1 ion) to be at 485.4 in the negative ion mode, and three representative characteristic daughter ions respectively at 441.4 and 397.3 and 321.5. The concentrated sample is separated by UPLC through Waters C18 2.1mm*100mm, 1.8 mum, and qualitative and quantitative analysis are conducted under MRM mode. The invention solves the insufficiencies of the HPLC and TLC detection methods, simplifies the extraction procedure, improves the detection sensitivity and accuracy, and has good repeatability, and is easy to be popularized and applied.