28 results about "Flavobacterium" patented technology

The invention relates to the technical field of ecological restoration, and particularly discloses a wetland ecological restoration technology. The wetland ecological restoration technology comprises the following steps: step 1) mixing bradyrhizobium angularis, flavobacterium, pseudomonas saccharophila, a nutrient substrate and water, and culturing for 7-9 days to obtain a culture solution; the method comprises the following steps of 1, preparing a culture solution, 2, cutting reed rhizomes into segments, and soaking the segments in the culture solution for 1-2 days to obtain pretreated rhizomes, and 3, inserting the pretreated rhizomes into sewage-covered soil for planting until the reeds grow to purify the sewage. The method has the advantages that the survival rate of the reeds in the sewage is increased, and wetland ecological restoration is accelerated.

The invention discloses a bacterial synbiotic beneficial to growth promotion of pasture in saline-alkali soil and application of the bacterial synbiotic. The bacterial synbiotic is a combination of bacterial strains NAU-RHO, NAU-MIC2 and NAU-FLA4 with 4-guanidinobutyric acid. The bacterial strains NAU-MIC2, NAU-FLA4, NAU-RHO are respectively classified and named as microbacterium laeviniform, Flavobacterium anhuiense and Rhodococcus oxyrhenium, and the research result shows that the bacterial synbiotics can be used for improving the salt tolerance and growth promoting effects of the bacteria on the medicago sativa and promoting the high yield of the medicago sativa in the saline-alkali soil.

The invention provides agaro-oligosaccharide as well as a preparation method and application thereof. The agaro-oligosaccharide is obtained by carrying out enzymolysis on a strain and agar; the bacterial strain comprises at least one of flavobacterium galactivorans and phycophagella albicans. The main component of the agaro-oligosaccharide is neoagarotetraose, and the agaro-oligosaccharide has lower polymerization degree, so that the agaro-oligosaccharide can be more easily absorbed by a human body.

The invention relates to a biological fermentation organic fertilizer based on crop residues and a fermentation method and application, and belongs to the technical field of organic fertilizer production.The fermentation method of the biological fermentation organic fertilizer based on the crop residues comprises the steps that firstly, the crop residues are pretreated to obtain a premixed material A and a premixed material B; mixing saccharomycetes, lactic acid bacteria, bacillus laterosporus, flavobacterium flagelloides, cellulase, xylanase and nattokinase to obtain an enzyme and bacterium mixture, composting the premixed material A and the premixed material B, and mixing with the enzyme and bacterium mixture for fermentation. According to the biological fermentation organic fertilizer based on the crop residues, the crop residues are pretreated through multiple means before fermentation, and a specific enzyme and bacterium mixing agent is mixed in the fermentation process to promote fermentation of the crop residues, so that after the organic fertilizer acts on soil, the content of organic matter in the soil can be increased, and the organic matter content in the soil can be increased; and heavy metals in the soil can be obviously reduced.

The invention relates to Flavobacterium cylindricum NY-1 and application thereof in prevention and treatment of microcystis water bloom, and belongs to the technical field of microorganisms, the Flavobacterium cylindricum NY-1 is preserved in Guangdong Microbial Culture Collection Center on February 11, 2026, the preservation number is GDMCC 67866, the Flavobacterium cylindricum NY-1 can efficiently and specifically target a microcystis population structure and drive the microcystis population structure to complete a'sedimentation-extinction 'process, and the Flavobacterium cylindricum NY-1 can be applied to prevention and treatment of microcystis water bloom. After the algal inhibition agent is applied to a natural water body with microcystis bloom for 72 hours, the algal inhibition rate reaches up to 99%, and the microcystis in the water body is almost completely removed, so that the microcystis bloom is efficiently treated.

The invention discloses an LAMP (loop-mediated isothermal amplification) detection primer group for flavobacterium psychrophilum and application of the LAMP detection primer group, and belongs to the technical field of molecular biology. The method comprises the following steps: firstly, searching a specific sequence which is completely not matched with other genomes according to a whole genome sequence of flavobacterium psychrophilum, and designing a specific LAMP detection primer group according to the specific sequence; the LAMP detection primer group comprises a pair of outer primers and a pair of inner primers, and the sequence compositions of the outer primers and the inner primers are respectively shown as SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 and SEQ ID NO: 4. The primer group is used for constant-temperature fluorescent LAMP detection of flavobacterium psychrophilum, and the result shows that the LAMP detection primer group designed by the invention has a good amplification effect on detection of target flavobacterium psychrophilum, and has the advantages of strong specificity, high sensitivity, convenience and rapidness in operation and the like when being used for detecting flavobacterium psychrophilum. The method is suitable for early screening, accurate diagnosis and epidemic situation detection of freshwater fish bacterial diseases caused by flavobacterium psychrophilum.

The invention discloses a method for preparing ganoderan rich in a mannogalactan structure from a Chryseobacterium bacterium GS-7 and Ganoderma lucidum YJ0724 and application of the ganoderan rich in the mannogalactan structure, and relates to the technical field of microbial fermention.The method comprises the following steps that a Chryseobacterium bacterium GS-7 seed solution is prepared, and a Ganoderma lucidum YJ0724 seed solution is prepared; the method comprises the following steps: a, simultaneously inoculating a seed solution of a Chryseobacterium bacterium GS-7 and a seed solution of Ganoderma lucidum YJ0724 into a fermentation medium for co-culture to obtain a fermentation solution containing Ganoderma lucidum polysaccharide rich in a mannan galactan structure; or b, filtering and degerming the fermentation liquor of the Chryseobacterium bacterium GS-7, and inoculating the fermentation liquor and the ganoderma lucidum YJ0724 seed solution into the culture medium for co-culture to obtain the fermentation liquor containing the ganoderma lucidum polysaccharide rich in the mannogalactan structure. The obtained ganoderma lucidum polysaccharide rich in the mannogalactan structure can improve the immunomodulatory activity.

This invention discloses a method for coupling physicochemical treatment and biodegradation of plastics. The method includes the following steps: S1. Mixing plastics, organic solvents, and a metal catalyst, and reacting to degrade them to obtain organic products. The metal catalyst is cobalt acetate and / or manganese acetate; S2. Biodegrading the organic products from step S1 using a mixed colony, which includes *Pseudomonas*, *Bacillus*, *Flavobacterium*, and *Rhodococcus*. This invention uses cobalt acetate and / or manganese acetate as metal catalysts. The catalytic oxidation of the metal catalyst can efficiently depolymerize traditional plastics, overcoming the bottleneck of C-C bond breaking that is difficult for microorganisms to achieve. It can successfully convert plastics into organic products mainly composed of dicarboxylic acids, benzoic acid, and terephthalic acid, and the mixed colony process completely mineralizes the organic products. This method achieves complete removal of plastics without producing any toxic or harmful substances.

The invention discloses a nitrogen-fixing and decay-promoting functional bacterium II471 and a screening method and application thereof, and the screening method comprises the following steps: collecting a sample to obtain an initial sample; the ammonium nitrogen high-temperature domestication method comprises the following steps: domesticating an obtained initial sample to obtain mother liquor; screening a used culture medium, an MD culture medium and a DSM88 culture medium; carrying out strain screening, separation and purification, sucking mother liquor, carrying out gradient dilution, and culturing to obtain an initial series of functional strains; the nitrogen-fixing and decay-promoting functional bacteria II471 disclosed by the invention are applied to compost, the functional strains are re-screened by a rapid screening method to obtain strains, the strains are identified, then a microbial inoculum is prepared, and the nitrogen-fixing and decay-promoting functional bacteria II471 disclosed by the invention is applied to compost. According to the bacillus flavobacterium thermophilus obtained by screening, the nitrogen loss is reduced, the formation of humus is promoted, and a high-temperature domestication method and a method for rapidly screening a high-temperature ammoxidation functional strain can be used for more rapidly obtaining a target functional strain.

The application discloses a kind of organic fertilizer based on biomass fermentation and its preparation method and application, belong to biological organic fertilizer technical field.The organic fertilizer based on biomass fermentation of the application includes the following raw materials: chicken manure 40-50 parts, rapeseed cake 20-30 parts, seaweed powder 15-20 parts, corn straw 10-15 parts, EM bacterial liquid 10-15 parts, compound microbial inoculant 6-10 parts, Rhizoma Cyperi 8-16 parts, mulberry branch 4-8 parts, water hyacinth 12-18 parts, mushroom residue 5-8 parts, wherein the compound microbial inoculant is composed of jelly-like bacillus, sand on flavobacterium, rhizosphere oligotrophic single-cell bacteria, which can quickly decompose organic matter, accelerate the production of small molecules, and be directly absorbed by plants and produce a variety of active substances during fermentation.The organic fertilizer of the application has a synergistic effect of multiple substances, significantly promotes photosynthesis of plants, improves nutrient production and transportation, effectively promotes growth of Chinese cabbage and improves quality of Chinese cabbage.

The present application provides a kind of microbial iron reduction coupling biomass carbon soil carbon sequestration-organic pollution control collaborative remediation method, the present application method is by selecting including nitrogen-fixing bacteria, phosphorus solubilizing bacteria and iron-reducing bacteria Compound bacterial species, mixed with biomass carbon to form microorganism-biomass carbon compound, apply to soil, regulate soil environmental conditions, to promote iron cycle and the carbon sequestration effect of biomass carbon.The specific steps include: selecting nitrogen-fixing bacteria consisting of Clostridium pasteurianum and soybean rhizobium, phosphorus solubilizing bacteria consisting of Bacillus subtilis, flavobacterium or Streptomyces, and iron-reducing bacteria consisting of Geobacter;Prepare biomass carbon;Mix compound bacterial species with biomass carbon;Application and adjust soil pH, moisture content and aeration.The method is suitable for various soils, especially soils susceptible to MCPA pollution, can effectively improve the soil carbon sequestration capacity and reduce environmental pollution.

The invention discloses a mixed microbial agent and application thereof in codonopsis pilosula planting. The composite flora is composed of phosphate solubilizing bacteria, phosphate solubilizing bacteria, phosphate solubilizing bacteria, phosphate solubilizing bacteria, phosphate solubilizing bacteria, phosphate solubilizing bacteria and phosphate solubilizing bacteria, potassium bacteria: pseudomonas aeruginosa and bacillus circulans; and nitrogen-fixing bacteria: autotrophic flavobacterium, clostridium pasteurianum and bradyrhizobium. Wherein the phosphate-solubilizing bacteria are wrapped by adopting a benzene diboronic acid-sodium alginate pH response material, the potassium-solubilizing bacteria are wrapped by adopting selenized sodium alginate-Ca < 2 + > salt ion response gel, and attapulgite is used as a carrier to form the slow-release bacterial agent. Phosphorus-solubilizing bacteria and potassium-solubilizing bacteria have a synergistic effect to maintain the optimal growth environment of codonopsis pilosula, trichoderma harzianum colonization is promoted through bacillus circulans exopolysaccharide to construct a synergistic network, and the acting effect of the fungicide is improved by combining dual-environment response release and boron-selenium element synergy. During application, the microbial inoculum and rotten stems and leaves of codonopsis pilosula are fermented to prepare a bacterial fertilizer for basal application, and then topdressing and flushing application are performed, so that the yield and polysaccharide content of codonopsis pilosula are remarkably increased.

The invention provides a soil carbon sequestration-organic pollution control synergistic remediation method by coupling microbial iron reduction with biomass charcoal, which comprises the following steps: selecting a composite strain comprising nitrogen-fixing bacteria, phosphate solubilizing bacteria and iron reducing bacteria, mixing the composite strain with biomass charcoal to form a microbial-biomass charcoal compound, applying the microbial-biomass charcoal compound to soil, regulating and controlling soil environmental conditions, and controlling organic pollution. The iron circulation and the carbon sequestration effect of the biomass charcoal are promoted. The method comprises the following specific steps: selecting nitrogen-fixing bacteria consisting of clostridium pasteurianum and rhizobium japonicum, phosphate solubilizing bacteria consisting of bacillus subtilis, flavobacterium or streptomyces, and iron reducing bacteria consisting of geobacterium; preparing biomass charcoal; mixing the composite strain with the biomass charcoal; and applying and adjusting the pH value, the moisture content and the air permeability of the soil. The method is suitable for various kinds of soil, especially soil easily polluted by MCPA, the carbon sequestration capacity of the soil can be effectively improved, and environmental pollution is reduced.

This invention relates to the field of resource and environmental technology, specifically to a method and application of an algae-bacteria hybrid system for treating high ammonia nitrogen wastewater. By regulating nutrient and operational parameters, a functional community is constructed and maintained, dominated by microalgae and supplemented by bacteria and cyanobacteria, with their relative abundance within a preset optimized range. A clear interspecific interaction network forms within the system: microalgae such as *Nostoc* and *Chlorella* exhibit mutualistic symbiosis, while bacteria such as *Flavobacterium* show antagonistic relationships with these microalgae. Positive and negative feedback mechanisms maintain community balance and system robustness. The three components complement each other: microalgae primarily drive photocatalytic nitrogen and phosphorus removal, cyanobacteria provide nitrogen fixation and stability, and functional bacteria participate in organic matter degradation and nitrogen cycling, achieving synergistic metabolism through material exchange. Microbial ecological analysis confirms that a stable community structure and internal interaction mechanisms are the core of efficient and stable ammonia nitrogen reduction. This technology offers precise regulation, stable operation, and is environmentally friendly and economical, and can be widely applied to the treatment of various types of ammonia nitrogen-polluted water bodies.

The application discloses a strain of Frateuria sp. and application thereof. The Frateuria sp. GZRe12 of the application was preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on May 17, 2022, and the preservation number is GDMCC No: 62474. The Frateuria sp. disclosed by the application is a new species of the genus, can effectively dissolve the insoluble inorganic phosphorus (Ca3(PO4)2), and the amount of dissolved phosphorus of the culture for 5 days is as high as 495.05+ / -11.58 mg / L, and the phosphorus dissolving performance is significantly better than that of most reported phosphorus dissolving strains. Therefore, the Frateuria sp. GZRe12 disclosed by the application can be used to improve the availability of phosphorus in soil, and has important application potential in promoting the growth and development of plants, preparing microbial agents and biological fertilizers and the like.

The present invention discloses a rapid detection kit and detection method for detecting psychrophilic Flavobacterium, and relates to the field of molecular biology technology. The present invention first obtains the whole genome sequence of psychrophilic Flavobacterium by high-throughput sequencing technology, compares the whole genome sequence one by one in the NCBI database, finds specific sequences that are completely mismatched with other genomes, designs specific primers based on the specific sequences, and finally screens out primers with high specificity through the specificity of other strain detection primers. Afterwards, a multiple PCR primer pair combination of psychrophilic Flavobacterium was designed and used to perform multiple PCR detection. The results show that the multiple PCR primer pair combination designed and screened by the present invention has a good amplification effect for detecting the target psychrophilic Flavobacterium, and the detection of psychrophilic Flavobacterium using it has significant specificity and high detection sensitivity.

The present invention belongs to the field of microorganisms, and particularly relates to a strain of Xanthobacter flavus and a method for synthesizing biological protein using the same. The specific technical solution is as follows: a strain of Xanthobacter flavus CIB1, which was deposited at the General Microbiological Center of the China Committee for Culture Collection of Microorganisms on May 19, 2022, with the deposit number: CGMCC NO. 24923. The present invention provides a method for a hydrogen-oxidizing bacterium to synthesize biological protein using CO2 and air. Under the condition of no addition of nitrogen source compounds, it can not only achieve the capture and reduction of CO2, but also obtain high-value-added biological protein, having the advantages of low cost and environmental friendliness.

The invention discloses a compound microbial agent for promoting corn growth based on rhizosphere micro food webs and application, and belongs to the technical field of agricultural microbial agents. The invention provides a compound microorganism bacterium agent for promoting growth and yield improvement of corn based on a rhizosphere micro food web. The compound microorganism bacterium agent is composed of bacterial strains of bacillus wiedmanni JN.2, bacterial strains of Flavobacterium ginossidimutans JN.3 and bacterial strains of Rosselomyces aquimaris L-3. The invention further provides a preparation method of the compound microorganism bacterium agent for promoting the growth and yield improvement of the corn based on the rhizosphere micro food web and a preparation method of the compound microorganism bacterium agent for promoting the growth and the yield improvement of the corn based on the rhizosphere micro food web and the preparation method of the compound microorganism bacterium agent for promoting the growth and the yield improvement of the corn based on the rhizosphere micro food web. By matching with a specific cultivation mode, namely, straw returning covering and sowing zone strip cultivation, the number of rhizosphere microbial populations can be remarkably increased, a rhizosphere micro food web can be directionally regulated and controlled, the soil structure is improved, nutrient release is accelerated, effective conversion of nutrients in the micro food web and transmission of the nutrients to corn plants are promoted, and corn growth and yield improvement are promoted; the method is environment-friendly and sustainable, convenient to use, simple to operate and high in economic benefit; the requirements on green and high-quality agricultural products are met.

This application provides a functional microbial composite double-layer coated controlled-release fertilizer and its preparation method, belonging to the field of controlled-release fertilizer technology. The functional microbial composite double-layer coated controlled-release fertilizer comprises, from the inside out, a core, a controlled-release layer, and a functional layer; wherein, the core is fertilizer granules; the controlled-release layer includes bio-based polymer materials and plasticizers; the functional layer includes functional microorganisms, stabilizers, and binders; the functional microorganisms include Rhizobium filamentosa, Flavobacterium tumefaciens, and Bacillus licheniformis.

The invention discloses a preparation method and application of wide-spectrum psychrophilic flavobacteria bacteriophage bacteriolysin. The psychrophilic flavobacteria bacteriophage bacteriolysin Ely-174 with high yield and high activity is obtained through heterologous expression. The bacteriolysin Ely-174 not only can be used for rapidly cracking flavobacterium psychrophilum, but also can be used for cracking other bacteria of bacteroides. After the bacteriolysin Ely-174 is stored for 30 days at the temperature of 4 DEG C, the cracking capacity is still 75.7%; and the cracking activity within 10 minutes after repeated freezing and thawing for 6 times under the storage condition of-20 DEG C is 92%. The acid and alkali resistance, the salt resistance and the serum resistance of the bacteriolysin Ely-174 can be suitable for a complex water area environment. Besides preventing and controlling bacterial diseases in the aquaculture industry, the bacteriolysin Ely-174 also has great application potential in the aspects of disinfection, preservation and the like of aquatic products.

The invention discloses a chlorohydrocarbon efficient synergistic degradation combined fungicide, a preparation method and application, and belongs to the technical field of microorganisms, the combined fungicide contains any one strain from rhodococcus or flavobacterium, and any one strain from bacillus, acinetobacter or comamonas. Through limitation of specific bacterium genus combination and artificial proportion optimization, the combined bacterium agent realizes a stable synergistic effect among different bacterium genus, the degradation efficiency is remarkably enhanced, and the 24-hour degradation rate of chlorinated hydrocarbon can be increased to 70% or above and is remarkably higher than that of a single bacterium system. Meanwhile, large-scale production can be achieved through the combined microbial agent, the overall cost is lower than that of a conventional scheme when the combined microbial agent is used for treating chlorinated hydrocarbon pollutants, additional secondary treatment is not needed after repairing is completed, and economic benefits are remarkable. When the combined microbial agent is used for treating chlorinated hydrocarbon pollutants, high-temperature treatment or chemical oxidation agents are not needed, and the combined microbial agent conforms to the development direction of current environmental protection laws and regulations and green restoration technologies.

The present invention discloses a cold-resistant composite bacterial agent for decomposing rice straw at low temperature and its application, belonging to the field of microbial technology. It is composed of Flavobacterium F1, Flavobacterium F2, Flavobacterium F3, Oxalobacterium 01, Oligotrophomonas S1 and Pseudomonas P1. There is no obvious antagonistic inhibition between the strains of the cold-resistant composite bacterial agent, and each strain has the ability to produce cellulase and filter paper enzyme under low temperature environment, among which the cellulase activity reaches up to 16.48U / mL, and the filter paper enzyme activity reaches up to 21.71U / mL. When the composite bacterial agent is used to degrade rice straw at low temperature, it can significantly reduce the mechanical strength of the straw and significantly reduce the cellulose, hemicellulose and lignin content in the straw. Under low temperature conditions, the cold-resistant composite bacterial agent can stably produce lignocellulose hydrolyzing enzymes, especially cellulase, accelerate the decomposition of rice straw and the release of nutrients, and has good development and application prospects.

The invention provides an application of Luteibacter rhizovicinus in degradation of oil and fat, and particularly provides an application of Luteibacter rhizovicinus in degradation of oil and fat. According to the present invention, the Luteibacter rhizovicinus is preserved in the China General Microbiological Culture Collection Center (CGMCC) on July 05, 2023, and the preservation number of the Luteibacter rhizovicinus is CGMCC No.27740; the provided flavibacter sylvestris can effectively degrade oil and fat.

The invention belongs to the technical field of microbial agents, and provides a methyl nutrition complex microbial inoculant and an application thereof in rice methane emission reduction, and the complex microbial inoculant comprises facultative methylobacterium, sphingobacterium, escherichia coli, variovorax, flavobacterium, sphingolipid, pseudoyellow microbacterium, Knoobrucella, sedimentary bacillus and terranean bacillus. The complex microbial inoculant disclosed by the invention can effectively reduce methane emission in rice planting, and has the functions of reducing emission, improving efficiency and promoting stable yield.

This invention discloses a method for promoting the growth and nutrient metabolism of radish tubers using earthworms in conjunction with nano-lanthanum oxide, belonging to the field of nano-agricultural regulation technology. The method of this invention, by simultaneously adding specific amounts of La2O3NMs and a specific density of *Pheretima spp.* in the soil, significantly promotes the tuber enlargement of cherry radishes, increases the net photosynthetic rate and sucrose content of cherry radish leaves, upregulates the expression of IAA biosynthetic genes in the radish epidermis, and ultimately promotes tuber epidermal growth. Furthermore, it increases the content of functional nutrients (D-tryptophan and sinapic acid) in the cherry radish tuber pulp and antioxidants (kaempferol, trifolin, calciferol 3-O-arabinoside, phlorizin) in the tuber epidermis, and increases the abundance of IAA-producing bacteria (flavobacterium) in the rhizosphere, but decreases the abundance of nitrogen cycle-related denitrifying microorganisms (Massilia), thereby increasing the rhizosphere nitrate nitrogen content and promoting nutrient absorption, growth, and physiological metabolism of cherry radish tubers.

The application discloses a kind of bacteria symbiotic of benefiting saline-alkali pasture growth and application thereof, the bacteria symbiotic is the combination of bacterial strain NAU-RHO, NAU-MIC2 and NAU-FLA4 and 4-guanidyl butyric acid.The bacterial strain NAU-MIC2, NAU-FLA4, NAU-RHO are respectively classified and named as Microbacterium laevaniformans, Flavobacterium anhuiense and Rhodococcus oxybenzonivorans research results show that bacteria symbiotic can improve the salt tolerance of bacteria to alfalfa and promote the high yield of alfalfa in saline-alkali soil.

The present invention provides a Flavobacterium strain for the production of antihypertensive peptides by cereal fermentation and its application, belonging to the technical field of functional microorganisms. The Flavobacterium strain J14-4 provided by the present invention has a deposit number of CCTCC NO: M20221649 and can be used for the fermentation of various cereals such as wheat, barley, rye, and buckwheat to produce polypeptides with antihypertensive activity. The technical solution provided by the present invention provides a new means for the preparation of novel biological antihypertensive drugs.

This invention provides a composite microorganism, a composite preparation, and its application for the aerobic composting and oil degradation of kitchen waste. The composite microorganism includes *Agrobacterium tumefaciens*. Agrobacterium swelling Curvular Pseudomonas Pseudomonas kneeling Near root flavobacterium Luteobacter rhizovicin Cylindrica monopermanente Simplellium cylindrosporum Fusarium moniliforme Fusarium proliferated ,and Simplellium woolly The provided compound microorganisms and compound preparations can be used for lipid degradation.