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Composite microbial preparation for treating watercourse organic matter

A technology of compound microorganisms and organic matter, applied in the field of microorganisms, can solve problems such as long time, weak degradation ability, secondary pollution, etc., and achieve the effects of low cost, growth inhibition, and odor elimination

Inactive Publication Date: 2017-05-10
DONGYING SHENGYU ENVIRONMENTAL PROTECTION TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to apply microbial preparations to river courses to improve the ecological environment of water bodies, to study the ability of compound microbial preparations to efficiently degrade organic waste, and to solve the technical problems of weak degradation ability, long time and secondary pollution in existing sewage treatment methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A kind of preparation step for a kind of composite microbial preparation for the treatment of river course organic matter is:

[0016] (1) Separate expansion culture of each microorganism:

[0017] Pseudomonas: Pseudomonas is cultured on the Pseudomonas culture medium at 28-30°C, firstly on a slant, then second-stage seed liquid culture, mixed fermentation culture until the bacterial concentration reaches 1-2×108 / mL. The Pseudomonas culture medium: peptone 5g, yeast peptone 1g, agar powder 20g, distilled water 1000mL, pH=7.8.

[0018] Vibrio: Vibrio cultured on the Vibrio culture medium at 28-30°C, firstly on a slant, then second-stage seed liquid culture, mixed fermentation culture until the bacterial concentration reached 1-2×108 / mL. The Vibrio culture medium: 5g of peptone, 1g of yeast peptone, 20g of agar powder, 1000mL of distilled water, pH=7.8.

[0019] Flavobacterium: Flavobacterium is cultured on the Flavobacterium medium at 28-30°C, first on a slant, then se...

Embodiment 2

[0031] A compound microbial preparation for the treatment of river organic matter, including compound microorganisms Pseudomonas, Vibrio, Flavobacterium, Pure Coccus nitrous, Thiobacillus oxidans, Bacillus subtilis, Bacillus licheniformis, Bacillus thiaminolyticus, Saccharomyces, denitrifying coccus, actinomycetes, described compound microorganism is made up of the raw material of following percentage by weight: Pseudomonas 15%, Vibrio 10%, Flavobacterium 10%, Purecoccus nitrous 15%, sulfur oxide Bacillus 13%, Bacillus subtilis 10%, Bacillus licheniformis 5%, Bacillus thiaminolyticus 5%, yeast 7%, denitrification coccus 5%, actinomycetes 5%. The above-mentioned bacterial strains used are conventional strains in the field, and can be purchased from the market or from China General Microorganism Culture Collection Center (CGMCC). In the compound microbial preparation, the total number of bacteria is more than 1 billion per gram, and it exists in the form of freeze-dried powder, ...

Embodiment 3

[0034]A certain river course in Jinan, Shandong is affected by the discharge of nearby domestic sewage all the year round. The COD is about 32mg / L, the BOD is about 8mg / L, the ammonia nitrogen concentration is about 1.7mg / L, the sulfide concentration is about 0.6 mg / L, the pH is 8-10, the water body is black and smelly, and the transparency is only 25cm. Take 10 tons of river water quality and apply a compound microbial preparation for river management described in this example. After the river water is diluted, it is evenly sprinkled in the water body. After 30 days of application, the COD is about 23 mg / L, and the BOD is about 5 mg / L. , transparency increased to 50cm; dissolved oxygen increased from 1.5mg / L to 4mg / L, ammonia nitrogen decreased to 0.85mg / L, sulfide concentration decreased to about 0.35 mg / L, and pH stabilized at 6.5-7.5; after 60 days, COD was About 17mg / L, BOD about 3mg / L, transparency increased to 80cm; dissolved oxygen increased from 4mg / L to 6.2mg / L, ammo...

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Abstract

The invention discloses a composite microbial preparation for treating watercourse organic matter. The composite microbial preparation is prepared from the following ingredients in percentage by weight: 10 to 20% of pseudomonas, 10 to 15% of vibrio, 10 to 15% of flavobacterium, 10 to 15% of pure nitrite coccus, 10 to 15% of thiobacillus thiooxidant, 10 to 20% of bacillus subtilis, 5 to 15% of bacillus licheniformis, 5 to 10% of aneurinibacillus aneurinilyticus, 5 to 10% of saccharomycetes, 5 to 10% of micrococcus denitrificans and 5 to 10% of actinomycetes. Compared with the prior art, the composite microbial preparation disclosed by the invention can rapidly decompose organic wastes, and effectively inhibits growth of harmful microorganisms; when the composite microbial preparation is put into polluted water, by utilizing metabolic activity of the microorganisms, concentration of pollutants in the water is reduced, the problems of blackening and smelliness of the water can be obviously improved, ammonia nitrogen content and total phosphorus content in the water are reduced, harmful substances such as hydrogen sulfide are decomposed, the water environment is improved, and transparency of the water is obviously improved; the composite microbial preparation has the advantages of simplicity for preparation, low cost and application to watercourse treatment, and has great economic and environmental-friendly significance for watercourse treatment.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a composite microbial preparation for treating organic matter in river courses. Background technique [0002] With the growth of population and the development of urban construction, a large number of human activities have had a huge impact on natural water bodies. A large number of pollutants are directly discharged into rivers, resulting in the continuous increase of organic matter, ammonia nitrogen and other harmful substances in water bodies, and the natural purification capacity of water bodies is seriously insufficient. . Adding microbial agents to eutrophic or polluted water bodies can effectively improve the purification ability of natural water bodies and resist the impact of water pollution. [0003] At present, the domestic treatment of lake pollution generally adopts the method of dredging or planting aquatic plants, both of which require large manpower and material...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C02F3/34C12R1/38C12R1/63C12R1/20C12R1/125C12R1/10
CPCC12N1/20C02F3/347C12N1/16
Inventor 韩旭东
Owner DONGYING SHENGYU ENVIRONMENTAL PROTECTION TECH CO LTD
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