2120 results about "Coccus" patented technology

The present invention relates to the field of bacterial polysaccharide antigen vaccines. In particular, the present invention relates to specific advantageous pnumococcal polysaccharide conjugates adjuvanted with 3D-MPL and substantially devoid aluminium-based adjuvant.

Enterococcus bacteria having enhanced tolerance to butanols have been isolated. The bacteria are useful for the fermentive production of butanol. New methods for the isolation of butanol tolerant Enterococcus are also provided.

The present invention relates to characterizing changes in mammalian bacterial gastrointestinal, cutaneous and nasal microbiota associated with antibiotic treatment and various disease conditions (such as asthma, allergy, obesity, metabolic syndrome, gastrointestinal reflux disease (GERD), eosinophilic esophagitis, gastro-esophageal junction adenocarcinomas (GEJAC), infections due to bacteria that are resistant to antibiotics, including Methicillin-resistant Staphylococcus aureus (MRSA), Clostridium difficile, vancomycin-resistant enterococci, etc.) and related diagnostic and therapeutic methods. Therapeutic methods of the invention involve the use of live bacterial inoculants that are capable of restoring healthy mammalian bacterial gastrointestinal, skin, and nasal microbiota.

The present invention provides a multivalent immunogenic composition having 15 distinct polysaccharide-protein conjugates. Each conjugate consists of a capsular polysaccharide prepared from a different serotype of Streptococcus pneumoniae (1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F, 22F, 23F or 33F) conjugated to a carrier protein, preferably CRM₁₉₇. The immunogenic composition, preferably formulated as a vaccine on an aluminum-based adjuvant, provides broad coverage against pneumococcal disease, particularly in infants and young children.

The invention relates to a compound microbial agent for degrading antibiotic and pesticide residues as well as preparation and application thereof, and belongs to the field of biotechnology and environmental protection. Multi-thallus compound microbial powder is prepared from the compound microbial agent according to the weight percentage of living microbes to the total amount of compound microbial powder as follows: 10%-15% of bacillus subtilis, 10%-15% of aspergillus niger, 10%-15% of bacillus mucilaginosus, 10%-15% of enterococcus faecalis, 8%-12% of bacillus licheniformis, 8%-12% of bacillus megaterium, 8%-12% of pseudomonas fluorescens, 5%-8% of lactobacillus plantarum, 5%-8% of bacillus polymyxin and 6%-8% of streptococcus thermophiles. The compound microbial agent has the effects of degrading antibiotic and pesticide residues, fermenting and composting organic matter, acting as functional fertilizer and repairing the environment, and can solve the problems of secondary pollution caused by antibiotic residues in culture feces and resource utilization of organic waste and realizes biodegradation of the antibiotic and pesticide residues in soil when applied to the agricultural ecological environment, thereby being of great value and practical significance in restoration of agricultural ecological environment and protection of human health.

The invention provides an Enterococcus faecium ANSE228 of which the collection number is CGMCC No.4082. The invention also provides application of the Enterococcus faecium ANSE228 to inhibition of salmonella pullorum and/or Escherichia coli and/or Staphylococcus aureus. The Enterococcus faecium ANSE228 is obtained by processes of repeated separation, purification, rejuvenation and the like, and has high biological activity, obvious probiotic property, high adversity resistance and the like. The invention also provides a microecological agent which contains the Enterococcus faecium ANSE228. When the microecological agent is added into drinking water and/or feeds for breeding animals, the Enterococcus faecium ANSE228 can be quickly activated and reproduced and a dominant beneficial flora can be formed after the Enterococcus faecium ANSE228 is fed into intestinal canals of the animals, and the Enterococcus faecium ANSE228 has the effects of reducing a harmful flora in the intestinal canals, adjusting microecological balance of the intestinal canals, substituting for medicaments such as antibiotic and the like, and improving weight increment of the animals and the utilization rate of the feeds.

The invention relates to aerobic denitrifying paracoccus denitrificans and application thereof. A bacterial strain provided in the invention is paracoccus denitrificans DN-3CGMCC No. 3658; the Paracoccus denitrificans can carry out aerobic denitrification by using nitrate nitrogen under both aerobic and oxygen-limited conditions and can also carry out heterotrophic nitrification-aerobic denitrification by using ammonia-N, and a total nitrogen removal rate on above mentioned occasions is greater than 90%. The paracoccus denitrificans provided in the invention has stable hereditary features, isapplicable to treatment of a variety of nitrogen-containing waste water and produces a good nitrogen removal effect.

The invention discloses a salt-tolerant microbial agent and a preparation method thereof. The microbial agent contains staphylococcus cohnii FSND-C, arthrobacter creatinolyticus FDN-1, flavobacterium mizutaii FDN-2, paracoccus denitrificans DN-3 and methylobacterium phyllosphaerae SDN-3. The microbial agent can achieve removal of ammonia nitrogen, total nitrogen and CODcr in the same reactor, has a good wastewater treatment effect, and can achieve short-cut nitrification and denitrification or simultaneous nitrification and denitrification while removing COD. Staphylococcus cohnii FSND-C can utilize various carbon sources, has certain salt tolerance, can be applied to the high-salinity wastewater treatment process, simultaneously can secrete a substance under environmental stimulus to enhance the flocculability of sludge, and further widens the application range of the microbial agent.

The current invention relates to the field of detection and identification of clinically important fungi. More particularely, the present invention relates to species specific probes originating from the Internal Transcribed Spacer (ITS) region of rDNA for the detection of fungal species such as Candida albicans, Candida parapsilosis, Candida tropicalis, Candida kefyr, Candida krusei, Candida glabrata, Candida dubliniensis, Aspergillus flavus, Aspergillus versicolor, Aspergillus nidulans, Aspergillus fumigatus, Cyptococcus neoformans and Pneumocystis carinii in clinical samples, and methods using said probes.

The present invention is in the field of pneumococcal capsular saccharide conjugate vaccines. Specifically, a multivalent Streptococcus pneumoniae immunogenic composition is provided with various conjugated capsular saccharides from different S. pneumoniae serotypes conjugated to 2 or more different carrier proteins, where the composition comprises serotype 19F capsular saccharide conjugated to diphtheria toxoid (DT) or CRM197, optionally wherein 19F is the only saccharide in the composition conjugated to diphtheria toxoid (DT) or CRM197.

The present disclosure provides for characterization of normal flora and identifying biomarkers in the gut of healthy, neruotypical subjects. Aspect of the disclosure provide for the characterization of the gut microbiome in ADS subjects, characterized by reduced richness and significant loss of the ‘Prevotella-like enterotype’ compared to neurotypical subjects. The relative abundance of genera Prevotella, Coprococcus, Prevotellaceae and Veillonellaceae are significantly lower in autistic children than in neurotypical children. Further, Prevotella, is one of the three main classifiers for the human enterotypes, along with Bacteroides and Ruminococcus. These three core genera are among main contributors in the principle component analysis. ‘Prevotella-like enterotype’ was absent in the autistic group, while neurotypical samples showed an even distribution among the three enterotypes. The present disclosure provides for an understanding the association between gut microbiota, health, and disease states, and provides for potential diagnostic and therapeutic targets.

The invention provides a multivalence pneumococcus capsular polysaccharide-protein conjugate composition and a preparation method thereof. The conjugate composition is prepared from capsular polysaccharides of pneumococcus of 24 different serotypes and a carrier protein in a covalence connection manner, wherein the 24 different serotypes are 1, 2, 3, 4, 5, 6A, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19A, 19F, 20, 22F, 23F and 33F. The conjugate composition is good in adsorption effect and stability, has multiple immunogenicity and protection properties aiming at invasion of pneumococcus of the 24 serotypes, and is superior to the low-valence pneumonia composition in the market, and the immune response is higher than that of uncombined compositions. By inoculating a multivalence pneumococcus capsular polysaccharide conjugate vaccine prepared from the conjugate composition, the inoculation injection times can be reduced, the immunization procedure can be simplified, and meanwhile human beings and animals can be effectively prevented from diseases resulted from the pneumococcus of the 24 serotypes, and the conjugate composition is wide in coverage range and good in immune effect.

The invention discloses a novel compound microorganism living bacteria agent. The agent comprises probiotics such as pediococcus, saccharomyces, bacillus and the like, wherein the total bacterial quantity is above 107 colony-forming units (cfu)/gram. The invention further discloses a method for preparing the novel compound microorganism living bacteria agent and applications of the novel compound microorganism living bacteria agent in preparation of organic sewage purification additives, deodorization fly dispelling agents, degreasing agents, pipeline blockage removing agents, feed additives and microorganism disease prevention agents. The novel compound microorganism living bacteria agent has the comprehensive effects of high removal rates to ammonia nitrogen, total nitrogen and total phosphorus, small sludge residues, energy saving, environmental friendliness and the like and the characteristics of convenience in use, safety and quickness in function, no secondary pollution, benefits to ecological protection, low treatment cost, good comprehensive profit and the like.

The invention relates to enterococcus faecium for feeding as well as a freeze-drying fungicide and applications thereof, and discloses enterococcus faecium LAB12 CGMCC (China General Microbiological Culture Collection Center) No.4847 which is grampositive cocci, has no spores, grows well on an MRS agar plate, forms a round bacterial colony with the diameter of 0.5-1mm within 48 hours and is used for feeding, and the bacterial colony is round, smooth and upheaved, and is a shape of grey white dewdrop; the enterococcus faecium grows in a facultative anaerobic condition; the growth temperature range is 10 DEG C-45 DEG C; the optimum growth temperature is 30 DEG C-40 DEG C; and the growth pH value is 4-10, and the optimum pH value is 6.0. The freeze-drying fungicide formed by the bacterial strain is nontoxic and harmless, is gastric juice resistant, is cholate resistant, has a high inhibitory effect for multiple harmful bacteria, has a long quality guarantee period, can be widely applied to birds and livestock breeding to strengthen the animal disease-resistant capability, and is expected to be the substitution of antibiotics for feeding.

A dry, stable and viable probiotic composition comprising, a probiotic microorganism and a dried plant powder, with the proviso that said composition is not a blended mixture of at least one biologically pure Pediococcus acidilactici probiotic culture and dried tomato powder at a weight ratio of 1:4 encapsulated in an effective amount in a gelatin capsule.

The invention relates to a biological denitrification method for salt-containing sewage, which comprises the following steps: adding a denitrification microbial inoculant into a biochemical sewage treatment system, and simultaneously starting the nitrification-denitrification biological denitrification treatment processes, wherein the sewage treatment temperature is 18-40 DEG C, the dissolved oxygen is 0.1-5 mg/L, and the pH value is 7.0-9.0. The denitrification microbial inoculant contains one or two of Staphylococcuscohnii FSDN-C, Arthrobactercreatinolyticus FDN-1 and Flavobacteriummizutaii FDN-2, and also contains one or two of Paracoccusdenitrificans DN-3 and Methylobacteriumphyllosphaerae SDN-3. The method provided by the invention can effectively treat high-salt-content sewage, enhances the adsorptivity and flocculence for sludge, has wide application range for wastewater quality and high impact resistance to salt-containing sewage, and obviously enhances the sewage treatment effect on the premise of removing pollutants, such as ammonia nitrogen, COD (chemical oxygen demand) and the like.

A Rhodococcus ruber Em (CGMCC No.0868) features that its cells, cell suspension, or immobilized cells can degradate the paraffin and polycyclic arylhydrocarbon because the paraffin and polycyclic arylhydrocarbon are used as its only carbon source and energy source for growing to generate the bioemulsifier of ester. Said emulsifier can obviously decrease the surface tension of aqueous solution, has strong emulsifying power to ester substance, and can increase the solubility of paraffin and polycyclic arylhydrocarbon in water, so it can be used for treating the oil-contained sewage and repairing the petroleum polluted soil.

The invention discloses microbial feed additive used for pigs, and a preparation process thereof; the prescription is as follows: 1 portion of bacillus licheniformis, 1 portion of bacillus subtilis, 2 portions of bifidobacterium, 2 portions of enterococcus faecalis, 2 portions of enterococcus faecium, 1 portion of boas-oppler bacillus, 1 portion of lactobacillus casei, 1 portion of lactobacillus lactis, 1 portion of plant lactobacillus, 1 portion of candida utilis, 1 portion of saccharomyces cerevisiae, 80kg to 100kg of panela, 3kg to 5kg of salt, 5kg to 8kg of vinegar, 3kg to 5kg of lysine, 1kg to 2kg of methionine, and 1000L of pure water; the weight of each portion of strain is 2g to 5g. The preparation process is as follows: strain preparation, nutrient broth preparation, inoculated fermentation and check. The invention reduces the quantity of the used individual strain, and the strain quality is safe and reliable. The production process is simple and feasible, the effect is obvious; therefore, the invention has promotion value.

The present invention discloses a short-cut nitrification-denitrification microbial agent. The microbial agent contains Arthrobacter creatinolyticus FDN 1, Flavobacterium mizutaii FDN 2, Paracoccus denitrificars DN 3 and Methylobacterium phyllosphaerae SDN3. The preservation register numbers of the four strains respectively are CGMCC No.3657, CGMCC No.3659, CGMCC No.3658 and CGMCC No.3660. With the microbial agent, the ammonia nitrogen removing, the total nitrogen removing and the CODcr removing in the same reactor can be achieved, the wastewater treatment effect is good, and the microbial agent is especially suitable for the purification treatment of the wastewater containing the ammonia nitrogen so as to really achieve the short-cut nitrification-denitrification or simultaneous nitrification and denitrification.

The invention discloses a multifunctional microorganism soil repairing agent as well as preparation and application thereof and belongs to the fields of biological techniques and environment protection. The multifunctional microorganism soil repairing agent is made of the following raw materials in parts by weight through uniform mixing: 30-60 parts of nano chitosan, 10-20 parts of humic acid, 10-20 parts of nano charcoal and 20-30 parts of a composite microbial agent, wherein the composite microbial agent is prepared from fresh benzene bacillus, B.sonorensis, lysinibacillus sphaericus, bacillus licheniformis, bacillus circulans, bBacillus thuringiensis, achromobacter denitrificans, saccharothrix australiensis, Rhodococcus zopfii, pseudonocardia sulfidoxydans and pseudonocardia dioxanivorans through purification, optimization, fermentation and drying dehydration. The multifunctional microorganism soil repairing agent disclosed by the invention is capable of replacing conventional physical and chemical repairing techniques, is capable of degrading complex pollutants, is wide in repairing range, is capable of preventing soil degradation and inhibiting diseases and insects in environments, and has the advantages of being good in repairing treatment effect, low in cost and economic and environment-friendly.