54 results about "Lactobacillus salivarius" patented technology

The invention belongs to the technical field of microorganisms, and particularly relates to combined lactobacillus salivarius and application thereof in female genital tract health. The preservation number of the strain is CCTCC NO: M20231719, and the strain has good bacteriostatic ability and dendritic cell (DC) maturation / activation stimulation ability, has no potential safety problem, can regulate vaginal micro-ecological balance, prevent infection caused by exogenous bacteria, effectively improve tumor immunosuppression microenvironment, and has good application prospects. And the method has very important significance on prevention / treatment of vaginal dysbacteriosis patients, gynecological tumors and precancerous lesions thereof.

The invention relates to genetic engineering combined lactobacillus salivarius and application of the genetic engineering combined lactobacillus salivarius. The strain is classified and named as combined lactobacillus salivarius CTMT2, and the preservation number of the strain is CCTCC (China Center for Type Culture Collection) M 2025547. After an NIAAA model is injected into the stomach of an alcoholic liver mouse, the acetaldehyde level of the liver of the mouse is obviously reduced, and the alcoholic fatty liver symptom of the mouse is obviously relieved; after long-term intragastric administration of a high-glucose and high-fat modeling mouse, the acetaldehyde level in excrement of the mouse can be obviously reduced, the progress of liver fibrosis is improved, and good application prospects are achieved.

This invention discloses a fermentation process and application of wheat germ, belonging to the field of feed additive technology. The fermentation process includes the following steps: mixing Bacillus coagulans powder (accession number CGMCC No. 1.17145), Lactobacillus salivarius powder (accession number CCTCC NO: M 2022848), and steam-sterilized and cooled wheat germ; performing solid-state fermentation in a sealed environment; and then sterilizing with ozone after fermentation to obtain fermented wheat germ. This invention produces highly nutritious fermented wheat germ through simple steps. This fermented wheat germ can be used as a feed additive for laying hens, improving their production performance.

Use of a composition comprising bacteria for odor reduction in the treatment of symptoms of tissue necrosis, wound secretions or putrid wounds or a disease selected from the group consisting of ozena, hyperhidrosis, osmidrosis, chronic urinary tract infections, chlamydial infections and halitosis; wherein the composition comprises the bacteria Lactobacillus acidophilus TYCA15, Lactobacillus salivarius, Bifidobacterium animalis, Bifidobacterium bifidum and Bifidobacterium longum in the ratio 1:1:1:1:1; and the composition totals 10 9 bacteria per gram of the composition.

The invention discloses a method for improving egg shell quality in the later egg laying period of laying hens by adding intestinal probiotics into feed, and belongs to the technical field of healthy poultry breeding. According to the method, specifically enriched lactobacillus salivarius is screened based on correlation analysis of intestinal flora and eggshell quality. The strain is added into basic ration of laying hens in the later egg laying period according to a certain proportion (preferably 150 mg / kg feed) in the form of bacterial powder with the viable count not lower than 1 * 10 < 9 > CFU / g, and the laying hens are continuously fed for at least 8 weeks. According to the method, the laying rate and the average daily egg weight of the laying hens in the later laying period can be remarkably increased, the eggshell thickness and strength are effectively improved, and the egg breaking rate is reduced. The strain screening method provided by the invention is scientific, clear in application effect, safe and free of residues, and an effective green solution is provided for solving the problem that the eggshell quality is reduced in the later egg laying period of laying hens.

Disclosed herein are methods and compositions related to probiotic formulas. These formulas comprise the bacterial strain Lactobacillus crispatus WZ-12 or a derivative thereof. Other strains which can be included in the composition include, but are not limited to, Lactobacillus salivarius 1-14, or Lactobacillus reuteri 2-2, and derivatives thereof. These compositions can be used in a variety of methods to treat and / or ameliorate diseases, such as colonization of antibiotic-resistant bacteria in the host organism. Also disclosed herein are methods of treating those subjects in need thereof by administering the compositions disclosed herein.

The present invention relates to a Lactobacillus salivarius strain deposited at the Spanish Type Culture Collection (CECT) under the accession number 5713, or a mutant thereof, for use in the treatment and / or prevention of infertility or recurrent miscarriage in a female subject. Furthermore, the present invention relates to an in vitro monitoring method for monitoring the therapeutic effect of the treatment of infertility or recurrent miscarriage in a female subject with a Lactobacillus salivarius strain deposited at the CECT under the accession number 5713, or a mutant thereof.

The application discloses a cat oral cavity nursing probiotic composition and application thereof, and the probiotic composition comprises animal bifidobacterium and lactic acid bacteria, wherein the lactic acid bacteria is any one or a combination of multiple of salivarius, lactobacillus casei, enterococcus faecalis, lactobacillus rhamnosus, lactobacillus plantarum, lactobacillus acidophilus and lactobacillus reuteri; compared with single probiotic, the probiotic composition has better effect on oral cavity odor removal, can effectively improve cat oral cavity source halitosis, and can also reduce dental plaque formation, relieve gingivitis and dental caries, and can be applied to preparation of a cat oral cavity odor cleaner, and has good application prospect.

The invention relates to the technical field of microbial preparations, and discloses a lactobacillus salivarius preparation, which comprises a core material and a wall material, the core material comprises a lactobacillus salivarius suspension and a protective agent solution, and the wall material is 1-3% by mass of a sodium alginate aqueous solution or 5-10% by mass of a whey protein aqueous solution. The protective agent solution comprises the following components in percentage by mass: 5-8% of glycerol, 10-15% of skim milk powder, 0.3-0.8% of sodium ascorbate and 6-10% of trehalose. By systematically screening and optimizing the components and proportion of the protective agent, an efficient protective system is constructed, the survival rate of thalli in the freeze-drying process and the stability of the preparation in long-term storage are remarkably improved, the preparation can quickly recover and maintain the healthy acidic environment of vagina, and the preparation has a good application prospect. Breeding of various common harmful bacteria is remarkably inhibited, and the specific effects of adjusting vagina micro-ecological balance and preventing and assisting in treating related gynecological inflammation are achieved.

The invention belongs to the technical field of biological medicine, and particularly relates to a preparation method of a probiotic hydrogel material and application of the probiotic hydrogel material in treatment of acute radioactive intestinal injury. In order to solve the clinical key problem of radioactive intestinal injury, a novel biological material TG-CCS-coated DY802 of thiolated synchronously modified chitosan hydrogel TG-CCS loaded with'localized high-response 'lactobacillus salivarius DY802 is constructed. The synthesis process of the material is synergistic and time-saving, has excellent biological safety and tumor inhibition experiment performance, and can comprehensively repair four barriers of the intestinal tract: ROS is removed, and tight junction protein is protected to repair the physical barrier; forming a hydrogel protective layer to reinforce the chemical barrier; the flora diversity is improved, and the prebiotic effect is exerted to repair the biological barrier; the sphingomyelin metabolism and ceramide abundance are regulated and controlled, the abnormal infiltration of NK cells is inhibited, and the immune barrier is remodeled. Therefore, the probiotic hydrogel material disclosed by the invention is clear in treatment mechanism and remarkable in clinical transformation potential and application value.

The application discloses a pig intestinal lactobacillus salivarius with antimicrobial activity and application thereof, relates to the technical field of microorganisms, and is named pig-LS2023. The strain can produce specific surfactants, the surfactants have antibacterial and antiviral activities, and have the advantages of biodegradability, non-toxicity and stability. The results of antimicrobial experiments show that the inhibition rates of the produced surfactants on pig escherichia coli and streptococcus are 95.06% and 81.80% respectively, and the surfactants have obvious inhibitory effect on PCV2. The research results show that the pig-LS2023 strain is expected to become a candidate probiotic strain for improving the immune state of pigs and producing a new type of anti-infection drug substitute.

The invention discloses application of 2-hydroxycinnamic acid in treatment of gastric cancer, according to the application, the influence of supernate of lactobacillus salivarius or lactobacillus crispatus on gastric cancer cell apoptosis is detected through a flow technology, and the 2-hydroxycinnamic acid is found to be capable of promoting the gastric cancer cell apoptosis and inhibiting the activity of gastric cancer organs. A metabolite in a supernatant of lactobacillus salivarius CICC23175 or lactobacillus crispatus CGMCC1.2743 screened by non-target metabolite omics contains 2-hydroxycinnamic acid, and the gastric cancer cytotoxic resistance of 2-hydroxycinnamic acid is deeply studied to find that the 2-hydroxycinnamic acid not only has the effects of inhibiting gastric cancer cell proliferation, colony formation and cell migration, but also has the effects of inhibiting gastric cancer cell proliferation, colony formation and cell migration. In addition, the 2-hydroxycinnamic acid can also increase the proportion of cells in a G1 / G0 period of a cell cycle, promote apoptosis of gastric cancer cells, and find that the inhibition effect of the 2-hydroxycinnamic acid on proliferation of gastric cancer organs is integrally higher than that of normal gastric epithelium organs.

The invention discloses a primer pair for detecting combined lactobacillus salivarius, a kit, a detection method and application, and belongs to the technical field of microbiological detection. In order to solve the problems of low detection efficiency and high cost of the traditional detection method of the combined lactobacillus salivarius in the prior art, the invention provides the primer pair, the kit and the detection method for detecting the combined lactobacillus salivarius, the primer pair and the kit can specifically judge whether the combined lactobacillus salivarius is the combined lactobacillus salivarius, and the detection result is accurate and reliable; the detection method is simple and easy to implement, the fastest detection time of the combined lactobacillus salivarius is only 24 hours, the detection efficiency is improved, and meanwhile, the detection cost is reduced. The primer pair, the kit and the detection method provided by the invention have great application value for judging whether the fermented dairy product or the fermented beverage product is added with the saliva combined lactobacillus or not.

The invention belongs to the technical field of microorganisms, and particularly relates to a method for promoting generation of cyclic dipeptide in porphyra haitanensis fermentation liquor by using a compound fermentation inoculant. The compound fermentation inoculant is prepared by mixing a strain of saliva combined lactobacillus separated and purified in a laboratory and a strain of saccharomyces cerevisiae according to different proportions, and the compound fermentation inoculant is used for fermenting the porphyra haitanensis, so that the generation of cyclic dipeptide can be promoted, and the content of cyclic dipeptide in the porphyra haitanensis fermentation liquid is increased. The method provided by the invention is simple, convenient, feasible, green and safe, not only can improve the nutritional value of the porphyra haitanensis fermentation liquor, but also contributes to promoting the development of healthy porphyra haitanensis fermentation products and the high-quality development of the porphyra haitanensis fermentation industry.

This invention discloses a *Ligilactobacillus salivarius* YS-SA09, with accession number CGMCC NO.37763 and Latin name *Ligilactobacillus salivarius*. This invention also discloses a *Citrus aurantium* ferment, obtained by fermenting *Citrus aurantium* with the aforementioned *Ligilactobacillus salivarius* YS-SA09. This invention further discloses the applications of *Ligilactobacillus salivarius* YS-SA09 and the *Citrus aurantium* ferment, which can effectively improve sleep in mice with insomnia models, effectively shorten sleep latency, and prolong sleep onset time; effectively improve depressive-like behavior in mice with CUMS models; significantly reverse the decrease in neurotransmitter levels caused by CUMS in mice, effectively increase the levels of GABA, 5-HT, DA, and NE in the serum of depressed mice; reduce serum TNF-α and IL-6 levels, and improve inflammatory factor levels.

The invention discloses application of lactobacillus salivarius in construction of an esophageal squamous cell carcinoma immunotherapy curative effect prediction model, a prediction method and a prediction device, and relates to the technical field of immunotherapy curative effect prediction. The lactobacillus salivarius has extremely high efficacy of predicting the curative effect of the esophageal squamous cell carcinoma immunotherapy and has the technical advantage of high accuracy in predicting the curative effect of the esophageal squamous cell carcinoma. According to the method, personalized treatment guidance can be provided for immunotherapy, whether a patient is suitable for immunotherapy or not can be judged according to the level of lactobacillus salivarius, the curative effect and accuracy of immunotherapy are improved, and unnecessary side effects and treatment cost are avoided. The lactobacillus salivarius can more accurately reflect an immune escape mechanism in a tumor microenvironment, and has a higher prediction value than PD-L1 expression. In addition, as a predictive marker, the lactobacillus salivarius provided by the invention can realize non-invasive detection.

The invention discloses combined lactobacillus salivarius and application of the combined lactobacillus salivarius in fermentation of bee pollen. The invention relates to a strain of combined lactobacillus salivarius XIF24, and the preservation number of the combined lactobacillus salivarius XIF24 is CCTCC (China Center for Type Culture Collection) NO: M 20252062. When the saliva is combined with the lactobacillus XIF24 to generate bee pollen, characteristic flavor substances including hexanoic acid and ethyl hexanoate can be produced, and due to the coexistence of hexanoic acid and residual ethyl hexanoate, the fermented bee pollen is endowed with attractive fruity flavor by enhancing the volatility and smell perception intensity of aroma substances; the acetoin is high in yield, and the fermented bee pollen is endowed with attractive sweet taste and milk taste; the bee pollen is endowed with attractive rose fragrance, so that the bee pollen has a wide application prospect.

The application discloses lactobacillus salivarius CCFM1266 capable of improving the content of nicotinic acid in fermented milk, and belongs to the technical field of microbial fermentation. The application discloses lactobacillus salivarius CCFM1266 capable of increasing the content of nicotinic acid in fermented milk, and a composite starter is prepared based on lactobacillus salivarius CCFM1266. The lactobacillus salivarius CCFM1266 provided by the application can utilize fructooligosaccharide and mannooligosaccharide as fermentation substrates to prepare fermented milk rich in nicotinic acid. In the fermentation system containing fructooligosaccharide and / or mannooligosaccharide: (1) the number of bacteria of lactobacillus salivarius is significantly increased; and (2) the content of nicotinic acid is significantly increased. Therefore, the lactobacillus salivarius CCFM1266 has great application prospect in the preparation of fermented milk products.

The application discloses a lactobacillus salivarius ZY-01 with anti-salmonella and anti-inflammatory repair functions, the strain has been preserved in the China General Microbiological Culture Collection Center on March 9, 2026, and the preservation number is CGMCC No. 37873. The strain can inhibit salmonella, escherichia coli and staphylococcus aureus, can reduce the expression of IL-6, IFN-gamma and TNF-alpha pro-inflammatory factors by inhibiting the JAK-STAT signal pathway, and can promote the expression of IL-10 anti-inflammatory factors and EGF, EGFR and TGF-beta1 mucosal repair factors, so that intestinal anti-inflammation and mucosal repair are realized. The lactobacillus salivarius ZY-01, bacterial liquid, bacterial powder and fermentation product thereof can be used as core raw materials of animal feed additives, micro-ecological preparations and veterinary drugs, are used for preventing and controlling salmonella infection and improving intestinal health of animals in animal breeding, and have a wide application prospect.

The application discloses application of a fermented Gastrodia elata water extract in preparation of a product for preventing and treating androgen alopecia, and belongs to the technical field of probiotic fermentation and application, wherein the fermented Gastrodia elata water extract is prepared from fermented Gastrodia elata by Lactobacillus salivarius AACE1. The fermentation process realizes biological transformation of Gastrodia elata raw medicinal materials, reduces potential irritation of the Gastrodia elata, and makes the product more suitable for long-term scalp care. Animal model efficacy evaluation of the water extract proves that the water extract can significantly improve androgen alopecia symptoms and has a remarkable effect on preventing and treating androgen alopecia.

The application discloses a postbiotic prepared from saliva combined lactobacillus and having the function of improving expression of host antibacterial peptide and immunoglobulin and application thereof, and belongs to the technical field of microorganisms. The saliva combined lactobacillus has the ability of regulating host immunity and improving host oral cavity anti-pathogenic bacteria infection, can significantly up-regulate expression of human oral cavity keratinocyte antibacterial peptide HBD-2 gene and protein in vitro, can significantly up-regulate expression of DEFB3 (homolog of human HBD-2) and secreted immunoglobulin SIgA in mice in vivo, and can be used for preparing a series of products for enhancing host oral cavity immunity and resisting oral cavity pathogenic bacteria infection, and has great application prospect.

The application discloses saliva combined lactobacillus and application thereof in preparation of medicine for relieving Parkinson's disease, and belongs to the field of microbial medicine. The strain is screened from healthy human excrement and has the activity of preventing and / or relieving Parkinson's disease. The strain is gram-positive non-spore bacillus, has no hemolytic activity and no cytotoxicity, meets the safety standard of probiotics, and can be orally administered for a long time, thereby avoiding the side effects such as liver and kidney damage and gastrointestinal reaction caused by chemical drug treatment of Parkinson's disease. Further researches show that the saliva combined lactobacillus NCU-41 and its metabolites can enhance the antioxidation defense ability in the brain by activating the AKT / Nrf2 signal pathway.

This invention belongs to the field of intestinal microbial technology, and specifically relates to a strain of *Lactobacillus salivarius* D3-8 and its application in the fermentation preparation of donkey-hide gelatin peptides. This strain was isolated from the intestines of healthy humans and is deposited at the China Center for Type Culture Collection (CCTCC), accession number CCTCC NO: M 20251921. *Lactobacillus salivarius* D3-8 can ferment and degrade donkey-hide gelatin, a large-molecule traditional Chinese medicine, into a series of low-molecular-weight donkey-hide gelatin peptides, showing broad application prospects in the fermentation and processing of donkey-hide gelatin and the preparation of donkey-hide gelatin peptides.

This invention provides a composition containing Lactobacillus salivarius HHuMin-U (KCCM13001P) for use in improving, preventing, or treating vaginitis or vulvitis. [Solution] A pharmaceutical composition for the prevention or treatment of vaginitis or vulvitis is provided, containing Lactobacillus salivarius HHuMin-U (KCCM13001P) as an active ingredient.

The invention relates to the technical field of probiotics, in particular to a lactic acid bacteria mixture for preventing and delaying heart valve calcification and application of the lactic acid bacteria mixture, and the lactic acid bacteria mixture comprises a saliva combined lactobacillus BR202 strain, a cheese paracasei BR176 strain, a bifidobacterium animalis subsp. Lactis BR621 strain and a plant lactobacillus PQQ-1 strain. According to the invention, human valvular interstitial cells are induced to generate osteogenic differentiation, so as to simulate pathological changes in calcified aortic valve diseases for evaluation, and the results of calcium salt deposition of the human valvular interstitial cells and osteogenic differentiation experiments of the human valvular interstitial cells prove that the human valvular interstitial cells can be used for simulating the pathological changes in the calcified aortic valve diseases. The lactic acid bacteria mixture can effectively delay calcium salt deposition of human valvular interstitial cells and osteogenic differentiation of the human valvular interstitial cells, so that the lactic acid bacteria mixture has the physiological activity function of preventing and delaying cardiac valve calcification, and can be used for preparing medicines or food compositions for preventing and delaying cardiac valve calcification.

The present application relates to the technical field of microorganisms, and particularly relates to a saliva host-associated lactobacillus V118 and application thereof. The saliva host-associated lactobacillus V118 has good inhibiting capacity on Candida albicans and mold, has strong adhesion capacity, has good anti-inflammatory effect, can effectively improve female vaginitis, has good safety, and has good application prospect. The strain can be used as a new type of probiotic resource, used for developing female vaginal health products, filling the market gap, and promoting the scientific development of female microecological health products.

The invention discloses an application of lactobacillus in treatment of gastric cancer. The lactobacillus is at least one of lactobacillus salivarius with the preservation number of CICC23175 and lactobacillus crispatus with the preservation number of CGMCC1.2743. The lactobacillus is at least one of lactobacillus salivarius with the preservation number of CICC23175 and lactobacillus crispatus with the preservation number of CGMCC1.2743. According to the present invention, the lactobacillus salivarius CICC23175 or the lactobacillus crispatus CGMCC1.2743, which has the excellent inhibition effect on the proliferation of SGC7901, AGS and MKN45 gastric cancer cells, is found; a mouse solid tumor model experiment shows that the lactobacillus salivarius CICC23175 or the lactobacillus crispatus CGMCC1.2743 can be used for inhibiting the growth of gastric cancer transplanted tumors. According to the invention, viable bacteria of lactobacillus salivarius CICC23175 or lactobacillus crispatus CGMCC1.2743 in lactobacillus are directly applied to treatment of gastric cancer for the first time, and a new direction and basis can be provided for treatment of gastric cancer.

The invention discloses application of lactobacillus salivarius LS08 in preparation of a medicine for preventing or treating intestinal inflammation, and belongs to the technical field of microorganisms. The preservation number of the lactobacillus salivarius LS08 disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No.22988. The lactobacillus salivarius LS08 can inhibit the DSS from inducing neutrophil to gather towards the intestinal tract of the zebra fish, so that a theoretical reference and a guidance basis are provided for developing the medicine for preventing or treating the intestinal inflammation by utilizing the lactobacillus salivarius LS08.

The application provides an antibacterial peptide composition, a preparation method and application, and comprises a composite probiotic core layer, an antibacterial lipopeptide loading layer and a CaCO3 nanocrystal buffered alginate / pectin composite microcapsule coating; the composite probiotic layer comprises a bacteria slurry composed of Ruminococcus lactaris CY12 and S. salivarius 7247 at a ratio of 1:1 in terms of viable bacterial number, and the total viable bacterial number is greater than or equal to 5.0x10 9 CFU / g; and the antibacterial lipopeptide layer comprises SEPs candidate peptides mined from human intestinal metagenome, and the total addition amount is 0.1%-0.5%. The antibacterial peptide composition, the preparation method and the application are used, gastric acid tolerance and colon targeting accuracy better than traditional pH-dependent coating are realized, natural synergy of maintenance and defense is realized, and excellent efficacy can be achieved without genetic engineering modification.