95 results about "Lactobacillus salivarius" patented technology

The invention relates to the technical field of probiotics, in particular to lactobacillus salivarius and application of the lactobacillus salivarius in regulating flora balance of digestive tracts of mutton sheep. The invention provides a lactobacillus salivarius KS1018, and the preservation number of the lactobacillus salivarius KS1018 is CCTCC (China Center for Type Culture Collection) NO: M 2025020. The lactobacillus salivarius KS1018 is a strain which is separated from dolang sheep rumen fluid and has the characteristics of high temperature resistance, acid resistance and cholate resistance, and has the advantages of low immunogenicity and high safety when being applied to mutton sheep. Due to the high-temperature-resistant characteristic, high stability can be kept in the feed mixing and processing process, and the application effect is further improved. The acid-resistant and cholate-resistant characteristics can reduce the influence of the digestive tract on the activity of the feed additive, so that the feed additive effectively plays a role in the digestive tract of the ruminant. The strain is good in palatability after being fed, and can effectively regulate digestive tract flora of ruminants, restore digestive tract flora balance and improve the disease-resistant potential of the ruminants.

Disclosed in the present invention is a probiotic. The probiotic comprises Parabacteroides distasonis with the microbial deposit number of CCTCC NO: M20222033, Limosilactobacillus fermentum with the microbial deposit number of CCTCC NO: M2023352, Lactobacillus salivarius with the microbial deposit number of CCTCC NO: M2023348, Enterococcus avium with the microbial deposit number of CCTCC NO: M2023350, and Bifidobacterium bifidum with the microbial deposit number of CCTCC NO: M2023349. The probiotic has good safety, has antioxidant activity, can inhibit pathogenic bacteria, can alleviate colon injury, can inhibit inflammation, and can alleviate diarrhea caused by chemotherapeutic drugs.

The invention belongs to the technical field of biomedical treatment and biological fermentation, discloses a metagen composition for effectively inhibiting oral dental caries and application thereof, and aims to solve the problems of great side effect, weak effect and unknown mechanism of the existing dental caries prevention and treatment means. The composition is prepared by mixing a metagen 1 which is prepared by fermenting lactobacillus salivarius Postbio-YY with a metagen 2 which is prepared by fermenting lactococcus lactis Postbio-F3 and lactobacillus paracasei Postbio-P6 step by step. In the preparation process, the active ingredients are reserved by accurately controlling the fermentation pH, time and pasteurization parameters. The composition can be prepared into multiple dosage forms such as toothpaste and the like, can remarkably inhibit the growth of streptococcus mutans and destroy the thallus structure, inhibits biofilm and exopolysaccharide synthesis by down-regulating fimI, wcaJ and wcae genes, is safe and free of side effects, and provides an efficient scheme for decayed tooth prevention and treatment.

The invention belongs to the technical field of microorganisms, and particularly relates to combined lactobacillus salivarius and application thereof in female genital tract health. The preservation number of the strain is CCTCC NO: M20231719, and the strain has good bacteriostatic ability and dendritic cell (DC) maturation / activation stimulation ability, has no potential safety problem, can regulate vaginal micro-ecological balance, prevent infection caused by exogenous bacteria, effectively improve tumor immunosuppression microenvironment, and has good application prospects. And the method has very important significance on prevention / treatment of vaginal dysbacteriosis patients, gynecological tumors and precancerous lesions thereof.

The invention relates to the technical field of marine microorganisms, in particular to application of combined lactobacillus salivarius in preparation of products for improving metabolism of alcohol and uric acid, the combined lactobacillus salivarius NHNK-603 is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2024416. Experiments show; the NHNK-603 has the effects of inhibiting the growth of klebsiella pneumoniae, inhibiting the production of alcohol, agglutinating the klebsiella pneumoniae, inhibiting the virulence gene of the klebsiella pneumoniae, promoting alcohol metabolism, reducing alcohol damage, tolerating alcohol, degrading uric acid, inhibiting the activity of xanthine oxidase, promoting uric acid excretion, promoting the proliferation of Alistipes indinctus and synthesizing hippuric acid; the thallus biofilm increases the function of colonization on mucoprotein and intestinal epithelial cells, and can be applied to preparation of products for improving metabolism of alcohol and uric acid.

The invention relates to genetic engineering combined lactobacillus salivarius and application of the genetic engineering combined lactobacillus salivarius. The strain is classified and named as combined lactobacillus salivarius CTMT2, and the preservation number of the strain is CCTCC (China Center for Type Culture Collection) M 2025547. After an NIAAA model is injected into the stomach of an alcoholic liver mouse, the acetaldehyde level of the liver of the mouse is obviously reduced, and the alcoholic fatty liver symptom of the mouse is obviously relieved; after long-term intragastric administration of a high-glucose and high-fat modeling mouse, the acetaldehyde level in excrement of the mouse can be obviously reduced, the progress of liver fibrosis is improved, and good application prospects are achieved.

This invention discloses a fermentation process and application of wheat germ, belonging to the field of feed additive technology. The fermentation process includes the following steps: mixing Bacillus coagulans powder (accession number CGMCC No. 1.17145), Lactobacillus salivarius powder (accession number CCTCC NO: M 2022848), and steam-sterilized and cooled wheat germ; performing solid-state fermentation in a sealed environment; and then sterilizing with ozone after fermentation to obtain fermented wheat germ. This invention produces highly nutritious fermented wheat germ through simple steps. This fermented wheat germ can be used as a feed additive for laying hens, improving their production performance.

The invention relates to a pharmaceutical composition for repairing oral gingivitis and a preparation method thereof, and belongs to the technical field of biological medicine, the pharmaceutical composition comprises a lactobacillus salivarius fermentation extract, a herba violae extract, golden cicada nymph extract peptide, guava polyphenol and resveratrol; the lactobacillus salivarius fermentation extract is obtained by carrying out aerobic fermentation and anaerobic fermentation on lactobacillus salivarius to obtain a fermentation product, and purifying and extracting the fermentation product through an XAD7HP macroporous adsorption resin column; the philippine violet herb extract is obtained by performing enzymolysis on whole philippine violet herb through pectinase and cellulase and then performing enzymolysis through compound protease to obtain zymolyte, and purifying and extracting the zymolyte through a compound adsorption column. The golden cicada nymph extracted peptide is obtained by purifying and extracting 5kDa zymolyte obtained by performing step-by-step enzymolysis on golden cicada nymphs through papain and trypsin through a Q agarose gel FF adsorption column. All the components are matched for use according to a certain proportion, so that the antibacterial coating is capable of precisely resisting bacteria, has efficient anti-inflammatory and anti-oxidation properties, and is safe and reliable.

The invention relates to the technical field of marine microorganisms, in particular to application of lactobacillus salivarius NHNK-603 in preparation of products for improving tooth color and gingivitis. The strain provided by the invention is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2024416. Experiments show; the NHNK-603 has the effects of inhibiting proliferation and biofilm formation of pigment-generating pathogenic bacteria, agglutinating the pigment-generating pathogenic bacteria, inhibiting melanin generation of the pigment-generating pathogenic bacteria, inhibiting hyaluronidase activity of the pathogenic bacteria, promoting growth of gingival fibroblasts, inhibiting gingival cell inflammation and apoptosis induced by the pigment-generating pathogenic bacteria, and inhibiting melanin generation of the pigment-generating pathogenic bacteria. And the product can be used for preparing products for improving tooth color and gingivitis.

Use of a composition comprising bacteria for odor reduction in the treatment of symptoms of tissue necrosis, wound secretions or putrid wounds or a disease selected from the group consisting of ozena, hyperhidrosis, osmidrosis, chronic urinary tract infections, chlamydial infections and halitosis; wherein the composition comprises the bacteria Lactobacillus acidophilus TYCA15, Lactobacillus salivarius, Bifidobacterium animalis, Bifidobacterium bifidum and Bifidobacterium longum in the ratio 1:1:1:1:1; and the composition totals 10 9 bacteria per gram of the composition.

The invention discloses a method for improving egg shell quality in the later egg laying period of laying hens by adding intestinal probiotics into feed, and belongs to the technical field of healthy poultry breeding. According to the method, specifically enriched lactobacillus salivarius is screened based on correlation analysis of intestinal flora and eggshell quality. The strain is added into basic ration of laying hens in the later egg laying period according to a certain proportion (preferably 150 mg / kg feed) in the form of bacterial powder with the viable count not lower than 1 * 10 < 9 > CFU / g, and the laying hens are continuously fed for at least 8 weeks. According to the method, the laying rate and the average daily egg weight of the laying hens in the later laying period can be remarkably increased, the eggshell thickness and strength are effectively improved, and the egg breaking rate is reduced. The strain screening method provided by the invention is scientific, clear in application effect, safe and free of residues, and an effective green solution is provided for solving the problem that the eggshell quality is reduced in the later egg laying period of laying hens.

Disclosed herein are methods and compositions related to probiotic formulas. These formulas comprise the bacterial strain Lactobacillus crispatus WZ-12 or a derivative thereof. Other strains which can be included in the composition include, but are not limited to, Lactobacillus salivarius 1-14, or Lactobacillus reuteri 2-2, and derivatives thereof. These compositions can be used in a variety of methods to treat and / or ameliorate diseases, such as colonization of antibiotic-resistant bacteria in the host organism. Also disclosed herein are methods of treating those subjects in need thereof by administering the compositions disclosed herein.

The present invention relates to a Lactobacillus salivarius strain deposited at the Spanish Type Culture Collection (CECT) under the accession number 5713, or a mutant thereof, for use in the treatment and / or prevention of infertility or recurrent miscarriage in a female subject. Furthermore, the present invention relates to an in vitro monitoring method for monitoring the therapeutic effect of the treatment of infertility or recurrent miscarriage in a female subject with a Lactobacillus salivarius strain deposited at the CECT under the accession number 5713, or a mutant thereof.

The application discloses a cat oral cavity nursing probiotic composition and application thereof, and the probiotic composition comprises animal bifidobacterium and lactic acid bacteria, wherein the lactic acid bacteria is any one or a combination of multiple of salivarius, lactobacillus casei, enterococcus faecalis, lactobacillus rhamnosus, lactobacillus plantarum, lactobacillus acidophilus and lactobacillus reuteri; compared with single probiotic, the probiotic composition has better effect on oral cavity odor removal, can effectively improve cat oral cavity source halitosis, and can also reduce dental plaque formation, relieve gingivitis and dental caries, and can be applied to preparation of a cat oral cavity odor cleaner, and has good application prospect.

The invention discloses a compound microorganism feed additive for promoting the growth of livestock and poultry. The compound microorganism feed additive comprises microorganism strains, natural plant components and auxiliary materials, the microbial strains comprise 8 to 12 parts of candida utilis, 5 to 9 parts of bacillus licheniformis, 2 to 4 parts of bacillus subtilis, 8 to 12 parts of aspergillus niger, 8 to 12 parts of lactobacillus delbrueckii, 8 to 12 parts of lactobacillus acidophilus, 8 to 12 parts of bifidobacterium and 3 to 7 parts of lactobacillus salivarius; the natural plant components comprise 0.5 to 2 parts of moringa seed powder; the auxiliary materials comprise 20 to 30 parts of cane sugar, 10 to 15 parts of fish meal, 2 to 3 parts of grease, 10 to 20 parts of soybean meal, 5 to 10 parts of pupa powder, 10 to 15 parts of zinc lactate, 1 to 2 parts of calcium gluconate, 3 to 5 parts of calcium carbonate and 2 to 4 parts of paeonol. Microbial strains in the compound microbial feed additive can generate various growth regulatory factors such as vitamins, amino acids and organic acids, so that the growth and development of livestock and poultry are stimulated, and the growth speed is increased.

In some embodiments, a composition comprising an effective amount of Bifidobacterium breve, Bifidobacterium bifidum, and Lactobacillus salivarius is provided. In some embodiments, the use of the composition in the prevention or treatment of atopic eczema in a subject is provided. In some embodiments, there is provided a method of preventing or treating atopic eczema in a subject, the method comprising the step of administering to the subject an effective amount of the composition. In some embodiments, there is provided a kit comprising: a composition of any one of the examples herein; and at least one container selected from the group consisting of capsules, pouches, packets, sachets, bottles, and blister packages. Other exemplary embodiments are described herein. In certain embodiments, the compositions are safe to use and effective to prevent or treat atopic eczema in a subject, especially an adult subject.

The invention discloses a pet deodorization disinfectant as well as a preparation method and application thereof. The deodorization disinfectant is prepared from a metabolite of a specific strain lactobacillus salivarius NJAUTYRGJ-Y (the preservation number is CCTCC NO: M 20242787), a plant extract, a disinfectant and salt components. The deodorant disinfectant can be directly sprayed on pet excrement, urine and living environment, and efficient deodorization and disinfection are realized. The deodorization disinfectant has a remarkable deodorization effect on excrement, urine and ammonia gas, and the deodorization effect lasts for a long time; the antibacterial agent has a good antibacterial effect on common pathogenic bacteria (staphylococcus aureus, escherichia coli and klebsiella pneumoniae), and also has a good disinfection effect on excrement, urine and air. In addition, the deodorant disinfectant has good safety, has no toxic or side effect, and is suitable for safe disinfection of pet living environment.

The invention provides combined lactobacillus salivarius LT017 and application thereof in prevention of decayed teeth and / or removal of dental plaque, and belongs to the technical field of microorganisms. The invention provides combined lactobacillus salivarius LT017, and the preservation number of the combined lactobacillus salivarius LT017 is CGMCC (China General Microbiological Culture Collection Center) No.30724 The invention further provides a preparation method of the combined lactobacillus salivarius LT017 bacterial powder, and an application of the combined lactobacillus salivarius LT017 bacterial powder in prevention of decayed teeth and / or removal of dental plaque.

The invention relates to the technical field of microbial preparations, and discloses a lactobacillus salivarius preparation, which comprises a core material and a wall material, the core material comprises a lactobacillus salivarius suspension and a protective agent solution, and the wall material is 1-3% by mass of a sodium alginate aqueous solution or 5-10% by mass of a whey protein aqueous solution. The protective agent solution comprises the following components in percentage by mass: 5-8% of glycerol, 10-15% of skim milk powder, 0.3-0.8% of sodium ascorbate and 6-10% of trehalose. By systematically screening and optimizing the components and proportion of the protective agent, an efficient protective system is constructed, the survival rate of thalli in the freeze-drying process and the stability of the preparation in long-term storage are remarkably improved, the preparation can quickly recover and maintain the healthy acidic environment of vagina, and the preparation has a good application prospect. Breeding of various common harmful bacteria is remarkably inhibited, and the specific effects of adjusting vagina micro-ecological balance and preventing and assisting in treating related gynecological inflammation are achieved.

The invention belongs to the technical field of biological medicine, and particularly relates to a preparation method of a probiotic hydrogel material and application of the probiotic hydrogel material in treatment of acute radioactive intestinal injury. In order to solve the clinical key problem of radioactive intestinal injury, a novel biological material TG-CCS-coated DY802 of thiolated synchronously modified chitosan hydrogel TG-CCS loaded with'localized high-response 'lactobacillus salivarius DY802 is constructed. The synthesis process of the material is synergistic and time-saving, has excellent biological safety and tumor inhibition experiment performance, and can comprehensively repair four barriers of the intestinal tract: ROS is removed, and tight junction protein is protected to repair the physical barrier; forming a hydrogel protective layer to reinforce the chemical barrier; the flora diversity is improved, and the prebiotic effect is exerted to repair the biological barrier; the sphingomyelin metabolism and ceramide abundance are regulated and controlled, the abnormal infiltration of NK cells is inhibited, and the immune barrier is remodeled. Therefore, the probiotic hydrogel material disclosed by the invention is clear in treatment mechanism and remarkable in clinical transformation potential and application value.

The application discloses a pig intestinal lactobacillus salivarius with antimicrobial activity and application thereof, relates to the technical field of microorganisms, and is named pig-LS2023. The strain can produce specific surfactants, the surfactants have antibacterial and antiviral activities, and have the advantages of biodegradability, non-toxicity and stability. The results of antimicrobial experiments show that the inhibition rates of the produced surfactants on pig escherichia coli and streptococcus are 95.06% and 81.80% respectively, and the surfactants have obvious inhibitory effect on PCV2. The research results show that the pig-LS2023 strain is expected to become a candidate probiotic strain for improving the immune state of pigs and producing a new type of anti-infection drug substitute.

The invention relates to the field of food, in particular to probiotic characteristics of lactobacillus salivarius JCF5 and a method for preparing Jersey yogurt through synergistic fermentation of the lactobacillus salivarius JCF5. The strain provided by the invention is named as Lactobacillus salivarius JCF5, and the Latin is Ligillactobacillussavarius. The invention further discloses a preparation method of the strain. The strain provided by the invention has the advantages that the strain is named as Lactobacillus salivarius JCF5; the strain is obtained by randomly selecting six newly-born Jersey and six Jersey-and-cattle hybrid calves with good health conditions from a pasture in Heilongjiang province, collecting fetal manure of the Jersey and six Jersey-and-cattle hybrid calves after the Jersey and cattle hybrid calves are born, and separating manure samples after the Jersey and cattle hybrid calves eat colostrum. The water-holding capacity and whey precipitation rate of the Jersey yoghourt can be remarkably improved, and the viscosity of the Jersey yoghourt can be increased. In addition, the strain has better acid resistance, cholate resistance and simulated gastrointestinal fluid resistance, does not show hemolysis capacity and gelatinase activity, has stronger antibacterial capacity and antibiotic sensitivity, and has the characteristics of probiotics. The application potential of the strain in fermenting the Jersey yogurt is fully explored, the quality of the Jersey yogurt is expected to be improved by changing the strain proportion of the leavening agent sold in the market, and a new leavening agent is provided for fermentation of the Jersey yogurt.

Provided in the present invention is a probiotic. The probiotic includes Parabacteroides distasonis with the microbial deposit number of CCTCC NO: M20222033, Limosilactobacillus fermentum with the microbial deposit number of CCTCC NO: M2023352, Lactobacillus salivarius with the microbial deposit number of CCTCC NO: M2023348, Enterococcus avium with the microbial deposit number of CCTCC NO: M2023350, and Bifidobacterium bifidum with the microbial deposit number of CCTCC NO: M2023349. The probiotic has good safety, has antioxidant activity, can inhibit pathogenic bacteria, can alleviate colon injury, can inhibit inflammation, and can alleviate diarrhea caused by chemotherapeutic drugs.

The invention discloses application of 2-hydroxycinnamic acid in treatment of gastric cancer, according to the application, the influence of supernate of lactobacillus salivarius or lactobacillus crispatus on gastric cancer cell apoptosis is detected through a flow technology, and the 2-hydroxycinnamic acid is found to be capable of promoting the gastric cancer cell apoptosis and inhibiting the activity of gastric cancer organs. A metabolite in a supernatant of lactobacillus salivarius CICC23175 or lactobacillus crispatus CGMCC1.2743 screened by non-target metabolite omics contains 2-hydroxycinnamic acid, and the gastric cancer cytotoxic resistance of 2-hydroxycinnamic acid is deeply studied to find that the 2-hydroxycinnamic acid not only has the effects of inhibiting gastric cancer cell proliferation, colony formation and cell migration, but also has the effects of inhibiting gastric cancer cell proliferation, colony formation and cell migration. In addition, the 2-hydroxycinnamic acid can also increase the proportion of cells in a G1 / G0 period of a cell cycle, promote apoptosis of gastric cancer cells, and find that the inhibition effect of the 2-hydroxycinnamic acid on proliferation of gastric cancer organs is integrally higher than that of normal gastric epithelium organs.

The invention discloses a primer pair for detecting combined lactobacillus salivarius, a kit, a detection method and application, and belongs to the technical field of microbiological detection. In order to solve the problems of low detection efficiency and high cost of the traditional detection method of the combined lactobacillus salivarius in the prior art, the invention provides the primer pair, the kit and the detection method for detecting the combined lactobacillus salivarius, the primer pair and the kit can specifically judge whether the combined lactobacillus salivarius is the combined lactobacillus salivarius, and the detection result is accurate and reliable; the detection method is simple and easy to implement, the fastest detection time of the combined lactobacillus salivarius is only 24 hours, the detection efficiency is improved, and meanwhile, the detection cost is reduced. The primer pair, the kit and the detection method provided by the invention have great application value for judging whether the fermented dairy product or the fermented beverage product is added with the saliva combined lactobacillus or not.

The invention belongs to the technical field of microorganisms, and particularly relates to a method for promoting generation of cyclic dipeptide in porphyra haitanensis fermentation liquor by using a compound fermentation inoculant. The compound fermentation inoculant is prepared by mixing a strain of saliva combined lactobacillus separated and purified in a laboratory and a strain of saccharomyces cerevisiae according to different proportions, and the compound fermentation inoculant is used for fermenting the porphyra haitanensis, so that the generation of cyclic dipeptide can be promoted, and the content of cyclic dipeptide in the porphyra haitanensis fermentation liquid is increased. The method provided by the invention is simple, convenient, feasible, green and safe, not only can improve the nutritional value of the porphyra haitanensis fermentation liquor, but also contributes to promoting the development of healthy porphyra haitanensis fermentation products and the high-quality development of the porphyra haitanensis fermentation industry.

This invention discloses a *Ligilactobacillus salivarius* YS-SA09, with accession number CGMCC NO.37763 and Latin name *Ligilactobacillus salivarius*. This invention also discloses a *Citrus aurantium* ferment, obtained by fermenting *Citrus aurantium* with the aforementioned *Ligilactobacillus salivarius* YS-SA09. This invention further discloses the applications of *Ligilactobacillus salivarius* YS-SA09 and the *Citrus aurantium* ferment, which can effectively improve sleep in mice with insomnia models, effectively shorten sleep latency, and prolong sleep onset time; effectively improve depressive-like behavior in mice with CUMS models; significantly reverse the decrease in neurotransmitter levels caused by CUMS in mice, effectively increase the levels of GABA, 5-HT, DA, and NE in the serum of depressed mice; reduce serum TNF-α and IL-6 levels, and improve inflammatory factor levels.

The invention discloses application of lactobacillus salivarius in construction of an esophageal squamous cell carcinoma immunotherapy curative effect prediction model, a prediction method and a prediction device, and relates to the technical field of immunotherapy curative effect prediction. The lactobacillus salivarius has extremely high efficacy of predicting the curative effect of the esophageal squamous cell carcinoma immunotherapy and has the technical advantage of high accuracy in predicting the curative effect of the esophageal squamous cell carcinoma. According to the method, personalized treatment guidance can be provided for immunotherapy, whether a patient is suitable for immunotherapy or not can be judged according to the level of lactobacillus salivarius, the curative effect and accuracy of immunotherapy are improved, and unnecessary side effects and treatment cost are avoided. The lactobacillus salivarius can more accurately reflect an immune escape mechanism in a tumor microenvironment, and has a higher prediction value than PD-L1 expression. In addition, as a predictive marker, the lactobacillus salivarius provided by the invention can realize non-invasive detection.

The invention discloses combined lactobacillus salivarius and application of the combined lactobacillus salivarius in fermentation of bee pollen. The invention relates to a strain of combined lactobacillus salivarius XIF24, and the preservation number of the combined lactobacillus salivarius XIF24 is CCTCC (China Center for Type Culture Collection) NO: M 20252062. When the saliva is combined with the lactobacillus XIF24 to generate bee pollen, characteristic flavor substances including hexanoic acid and ethyl hexanoate can be produced, and due to the coexistence of hexanoic acid and residual ethyl hexanoate, the fermented bee pollen is endowed with attractive fruity flavor by enhancing the volatility and smell perception intensity of aroma substances; the acetoin is high in yield, and the fermented bee pollen is endowed with attractive sweet taste and milk taste; the bee pollen is endowed with attractive rose fragrance, so that the bee pollen has a wide application prospect.

The application discloses lactobacillus salivarius CCFM1266 capable of improving the content of nicotinic acid in fermented milk, and belongs to the technical field of microbial fermentation. The application discloses lactobacillus salivarius CCFM1266 capable of increasing the content of nicotinic acid in fermented milk, and a composite starter is prepared based on lactobacillus salivarius CCFM1266. The lactobacillus salivarius CCFM1266 provided by the application can utilize fructooligosaccharide and mannooligosaccharide as fermentation substrates to prepare fermented milk rich in nicotinic acid. In the fermentation system containing fructooligosaccharide and / or mannooligosaccharide: (1) the number of bacteria of lactobacillus salivarius is significantly increased; and (2) the content of nicotinic acid is significantly increased. Therefore, the lactobacillus salivarius CCFM1266 has great application prospect in the preparation of fermented milk products.