119 results about "Lactobacillus fermentum" patented technology

This invention relates to the technical field of deep processing of sturgeon by-products, and particularly to a sturgeon tendon peptide with antioxidant and pancreatic lipase inhibitory activities and its preparation method. The preparation method includes the following steps: mixing sturgeon with water and crushing it to form a first slurry; adding Bacillus licheniformis alkaline protease to the first slurry for a first hydrolysis; adding Bacillus subtilis neutral protease to the first hydrolysate for a second hydrolysis; adding a complex enzyme to the second hydrolysate for a third hydrolysis to obtain a sturgeon tendon hydrolysate; filtering the sturgeon tendon hydrolysate to remove impurities to obtain a filtrate, which is then filtered again to retain polypeptides, thus obtaining the sturgeon tendon peptide; wherein the complex enzyme includes pectinase and amylase. Using this method, a sturgeon tendon peptide with high glycosaminoglycan content can be obtained, which exhibits pancreatic lipase inhibitory activity, antioxidant activity, Staphylococcus aureus inhibitory activity, and prebiotic effects that promote the proliferation of Lactobacillus plantarum and Lactobacillus fermentum.

The invention discloses an esophageal adenocarcinoma plasma circulation microbial marker combination, a reagent, a kit, a risk prediction model and application thereof, and belongs to the cross technical field of liquid biopsy and microbiomics. The esophageal adenocarcinoma plasma circulating microbial marker combination disclosed by the invention comprises enterobacteriaceae, cilia, prevotella, ackermania, lactobacillus fermentum and streptococcus. When the esophageal adenocarcinoma plasma circulating microbial marker combination is used for verifying esophageal adenocarcinoma patients and healthy controls, the AUC is 0.952, the sensitivity is 100%, the specificity is 80.00%, the accuracy is 91.43%, the positive predictive value is 86.96%, and the negative predictive value is 100%; the esophageal adenocarcinoma plasma circulating microbial marker combination can effectively distinguish esophageal adenocarcinoma patients from healthy people, is suitable for early screening of esophageal adenocarcinoma, and has remarkable clinical screening application value.

Provided are a mixture of three species of Lactobacillus fermentum strain and Lactobacillus plantarum strain and use thereof. A composition according to one aspect including, as an active ingredient, a mixture of Lactobacillus fermentum strains GB102 and GB103 and Lactobacillus plantarum strain GB104; or a lysate or culture solution thereof, or an extract of the culture solution, can be usefully used for the prevention or treatment of muscle-related disorders or obesity by alleviating the decrease in grip strength induced by aging or reducing the change in intestinal microbial environment induced by aging.

The invention discloses a compound bacterial agent for reducing blood sugar and blood fat, application of the compound bacterial agent and a method for preparing a fermented coconut milk beverage from the compound bacterial agent, belongs to the technical field of microorganisms, and particularly relates to the compound bacterial agent for reducing blood sugar and blood fat, the compound bacterial agent comprises fermented lactobacillus mucus AL-8 and lactobacillus paracasei DX-5, and the compound bacterial agent is prepared from the fermented lactobacillus mucus AL-8, the lactobacillus paracasei DX-5, the lactobacillus paracasei DX-5, the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5 and the lactobacillus paracasei DX-5. The preservation number of the lactobacillus mucus AL-8 is CCTCC (China Center For Type Culture Collection) NO: M20252735; the preservation number of the lactobacillus paracasei DX-5 is CCTCC (China Center For Type Culture Collection) NO: M20252736. According to the invention, the fermented coconut milk beverage is prepared by utilizing the inhibition effect of a blood sugar and blood fat reducing compound bacterial agent consisting of fermented lactobacillus mucus AL-8 and casei paracasei DX-5 on alpha-amylase and pancreatic lipase.

The invention belongs to the technical field of cosmetic raw materials, and particularly relates to shea butter fermentation liquor as well as a preparation method and application thereof. Lactobacillus plantarum, lactobacillus fermentum and lactobacillus gasseri in a specific proportion are adopted to construct a composite fermentation system, acid-resistant aspergillus niger lipase is combined, and shea butter is treated through a one-step fermentation process. By means of the synergistic effect of the three strains and the synergistic effect of the enzyme and the strains, efficient fermentation can be achieved, the content of active ingredients such as polysaccharide, amino acid and polypeptide in fermentation liquor is remarkably increased, meanwhile, the technological process is simple and stable, and the infectious microbe pollution risk is avoided. According to the fermentation liquor prepared by the process, on the basis of enhancing the original moisturizing and soothing effects of the shea butter, the problems of greasy skin feeling and poor permeation and absorption of the shea butter are effectively solved, the shea butter is further endowed with new functions of repairing skin barriers and balancing skin micro-ecology, and the application values of the shea butter and the fermentation liquor are greatly improved.

The invention discloses lactobacillus mucus YF28 and application thereof, and belongs to the technical field of microorganisms. The fermented lactobacillus mucus YF28 is separated from Hu sheep manure, is classified and named as the fermented lactobacillus mucus YF28, and has the preservation number of CCTCC (China Center For Type Culture Collection) NO: M 20252433. The fermented lactobacillus mucilaginosus YF28 has good tolerance in the gastrointestinal environment and can reach the intestinal tract to play a role. The bacterial strain has strong intestinal adhesion capacity, is easy to form a probiotic biological membrane, and is beneficial to intestinal health. The fermented lactobacillus mucus YF28 can promote peristalsis, enrich the abundance of Akkermansia bacteria and bacteriophage thereof, restore the water content of excrement to a normal level, and significantly promote intestinal health. The invention has an application prospect in the development of drugs or health-care foods with the function of relieving constipation and related gastrointestinal symptoms.

Provided is a novel Lactobacillus fermentum strain (Accession No. KCTC 14106BP) and a composition for preventing or treating a metabolic disease including the same. A Lactobacillus fermentum according to the present invention may inhibit fat accumulation and increase anti-inflammatory cells in white adipose tissue. In addition, the Lactobacillus fermentum may effectively inhibit fat accumulation in brown adipose tissue and in liver tissue. The Lactobacillus fermentum strain may exhibit excellent blood glucose improvement effect, and in particular, may reduce a fasting blood glucose level. The Lactobacillus fermentum strain may effectively ameliorate insulin resistance by improving glucose tolerance and increasing insulin sensitivity. Therefore, the Lactobacillus fermentum strain may be usefully used to prevent or treat a metabolic disease such as obesity.

The invention relates to a herbal acne-cleaning composition as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The invention relates to a herbal acne-removing composition. The composition comprises the following components in parts by weight: 1-3 parts of honeysuckle flower extract, 4-6 parts of mangnolia officinalis bark extract, 0.1-1 part of dandelion extract and 2-5 parts of fermentation extract, a fermentation strain used in the fermentation extract is lactobacillus fermentum, the preservation number of the lactobacillus fermentum is CGMCC No.21381, and fermentation substrate powder comprises amur cork powder and white willow bark powder. The herbal acne-removing composition disclosed by the invention has a remarkable acne-removing effect, and is mild and non-irritant.

The invention discloses combined probiotics for improving sarcopenia as well as a preparation method and application of the combined probiotics, and belongs to the field of probiotics. The invention relates to a combined probiotic for improving sarcopenia. The combined probiotic comprises a lactobacillus reuteri body, a lactobacillus johnsonii body, a lactobacillus fermentum body and a bifidobacterium adolescentis body. The combined probiotics disclosed by the invention can be used for remarkably improving the overall symptoms of sarcopenia model mice, including inhibiting weight loss, constipation symptoms and colonic mucosa destruction, prolonging load swimming time, improving muscle endurance, improving skeletal muscle atrophy, improving the expression of I-type slow muscle fiber protein MyHC I and promoting slow muscle fiber repair, and has the effects of preventing and treating sarcopenia. The invention discloses a probiotic intervention method for treating sarcopenia, which comprises the following steps of: preparing probiotics for treating sarcopenia, reducing the content of inflammatory cytokines in muscular tissues, increasing the content of hepatic glycogen, improving systematic metabolism of mice with sarcopenia and damaged serum markers, including lactic acid, lactic dehydrogenase, creatine kinase, creatinine, alanine transaminase ALT and aspartate transaminase AST, providing a new scheme for probiotic intervention for sarcopenia, and being used for preparing related functional products or intervention means.

The invention provides a preparation method of a fruit and carrot fermented beverage, and relates to the technical field of biological fermentation preparation. The preparation method of the fruit carrot fermented beverage comprises the following steps: selecting fresh fruit carrots, washing, steaming, pulping, filtering and sterilizing to obtain filtrate; adjusting the temperature to 30-40 DEG C, inoculating lactobacillus fermentum into the filtrate, performing anaerobic fermentation for 3-6 hours to obtain a fermentation product A, adjusting the temperature to 28-30 DEG C, inoculating saccharomyces cerevisiae into the fermentation product A, and performing anaerobic mixed fermentation for 4-9 hours to obtain a fermentation product B; according to the invention, lactobacillus fermentum is used for pre-fermentation of carrots, and mixed fermentation is carried out in combination with intervention of later-stage saccharomyces cerevisiae, so that olefinic terpene substances in the carrots are effectively reduced, the earthy smell in the carrot fermented beverage is improved, and the color, luster and fragrance can be improved on the basis of stably maintaining the clarity of the existing carrot fermented beverage; the comprehensive competitiveness of the carrot fermented beverage in the market is improved.

The invention provides a fruit juice fermentation inoculant, fermented guava juice and a preparation method, and belongs to the technical field of fermented beverages. The invention provides a compound fermentation inoculant capable of fermenting fruit juice, the compound fermentation inoculant is composed of lactobacillus plantarum and lactobacillus fermentum, the fermentation inoculant is applied to fermentation of guava fruit juice, beta-glucosidase secreted in the fermentation process can hydrolyze combined polyphenol substances in guavas, and then the guava fruit juice is fermented. The antioxidant is converted into high-activity antioxidants such as quercetin and gallic acid in a free state, so that the content of free phenol is increased by about 2 times, and the scavenging rate of DPPH free radicals is increased by more than 40%. The fermentation is carried out in an oxygen-free environment, the retention rate of vitamin C can reach 80% or above, the low pH value at the fermentation end point and organic acid jointly inhibit pathogenic microorganisms, and the product stability is guaranteed. Therefore, the guava juice prepared from the compound fermentation inoculant maximally releases the natural nutritional potential of guavas.

This invention belongs to the field of food fermentation technology, and specifically relates to a compound probiotic agent and its application in the preparation of fermented soy milk. This invention provides a compound probiotic agent composed of *Lactobacillus fermentum* NCPS LF01, *Pediococcus pentosaceus* NCPS PPE01, and *Lactobacillus acidophilus* NCPS LA01 in a volume ratio of 1-2:1:1, and applies this compound probiotic agent to the preparation of soy products, especially fermented soy milk. The beneficial effects of this invention are that fermented soy milk prepared using this compound probiotic agent has a rich flavor, and the total flavonoid and total phenolic content, as well as the in vitro DPPH free radical scavenging rate, are significantly superior to those of ordinary commercially available strains or other strains. In summary, this compound probiotic agent can effectively improve the retention level of active ingredients and antioxidant capacity of fermented soy milk, and has outstanding application value.

The invention provides a probiotic fermentation and bacterial powder compound addition chewable tablet and a preparation method thereof, and belongs to the technical field of microorganisms, the preparation method comprises the following steps: respectively adding water into apple peel, cerasus humilis fruits and aronia melanocarpa fruits, pulping, and mixing in proportion to obtain mixed fruit pulp; a mixed bacterium solution of lactobacillus plantarum LNJ-HQ-01 and lactobacillus fermentum BNCC194390 is added into the mixed fruit pulp, and fermentation is performed; freeze-drying and grinding the fermented fruit pulp to obtain freeze-dried fruit powder; the preparation method comprises the following steps: respectively activating lactobacillus plantarum LNJ-HQ-01, lactobacillus acidophilus BNCC186447 and lactobacillus rhamnosus ATCC7469, performing enlarged culture, taking bacterial sludge, uniformly mixing the bacterial sludge with a freeze-drying protective additive, and performing freeze-drying to obtain freeze-dried bacterial powder; and uniformly mixing the fruit powder, the freeze-dried bacterial powder and the medicinal auxiliary materials, and tabletting to obtain the probiotic chewable tablets. The probiotic chewable tablet is high in dietary fiber content, effective calcium content and total phenol content, high in oxidation resistance and suitable for calcium supplement of special groups such as diabetics and the like.

The application provides a Lactobacillus fermentum and a fermentation product thereof in application in prevention and control of fungal toxin pollution of agricultural products, and relates to the technical field of food safety prevention and control. Through research on the Lactobacillus fermentum with the preservation number CGMCC 1.15608, it is found that the strain and the fermentation product thereof have good prevention and control effect on fungal toxins, can effectively inhibit the formation of fungal toxin pollution in crops, and can be used for preparing a fungal toxin inhibitor.

The application discloses a plant lactobacillus strain and application thereof, and belongs to the technical field of microorganisms. The preservation number of the strain is CGMCC No. 33864. The strain has good tolerance to gastrointestinal juice and bile salts and has no hemolytic activity. Concentrated cell-free supernatant prepared from the strain can inhibit salmonella, including inhibiting the growth of salmonella, killing bacteria, inhibiting or removing biofilm. Animal experiments show that the strain and the concentrated cell-free supernatant thereof can prevent salmonella infection, alleviate weight loss caused by infection, maintain intestinal barrier integrity, reduce the level of pro-inflammatory factors, alleviate tissue damage, improve the expression of colon tight junction proteins and mucin, promote the generation of short-chain fatty acids, and restore the intestinal flora structure. The application further provides a microbial preparation containing the strain and / or the concentrated cell-free supernatant thereof. The strain and the microbial preparation can be used for preparing products for resisting salmonella, preventing salmonella infection or improving intestinal health.

This invention discloses a method for enhancing the color and aroma of whole-component fruit juice through synergistic fermentation with lactic acid bacteria, belonging to the field of food processing technology. The method uses ripe mangoes / pineapples as raw materials. After washing, pitting (peeling), and low-temperature jet milling to retain all components, the juice undergoes compound enzymatic hydrolysis and optimization of its uniformity. A compound strain composed of *Lactobacillus plantarum*, *Lactobacillus fermentum*, and *Lactobacillus helveticus* is then inoculated. The mixture undergoes constant-temperature anaerobic fermentation. The process is optimized using fuzzy mathematical sensory evaluation and response surface methodology, resulting in a sensory score of 91.67 for mango juice and 90.83 for pineapple juice. The retention rate of carotenoids is increased by 25%-30%, the lactic acid content reaches 2.97-26.45 mg / g, and the relative content of esters in volatile aroma components increases to over 35%. Simultaneously, the total sugar content decreases by 3.7%-24.0%. This method solves the problems of dull color, weak aroma, and nutrient loss in traditional whole-component fruit juices. The product combines natural functionality with a unique fermented flavor, making it suitable for industrial production.

The invention relates to the technical field of biological preparations, and discloses a periodontitis improving compound preparation containing fermented lactobacillus mucus and traditional Chinese medicine extracts, and the periodontitis improving compound preparation comprises the following raw materials: an oral preparation: fermented lactobacillus mucus CQPC202403 bacterial powder; the gel comprises scutellaria baicalensis, honeysuckle, salvia miltiorrhiza, liquorice and a hydrogel matrix; the preservation number of the lactobacillus mucus CQPC202403 in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC NO.31106, and the preservation date of the lactobacillus mucus CQPC202403 is June 27, 2024. The lactobacillus mucilaginosus adopted by the invention inhibits pathogenic bacteria through competitive rejection and immunoregulation; the traditional Chinese medicine extracts such as the radix scutellariae directly destroy bacterial pellicles, and the radix scutellariae and the traditional Chinese medicine extracts cooperate with each other, so that the antibacterial effect is enhanced on the premise of not causing drug resistance.

The invention discloses a leavening dough growth-promoting lactobacillus strain KUST4216 for shortening food fermentation time and producing p-cresol at high yield and application of the leavening dough growth-promoting lactobacillus strain KUST4216. The strain is classified and named as Levibacillus zyma KUST4216; the preservation number of the strain is GDMCC NO: 65660. The content of p-cresol in fermentation liquor is larger than or equal to 5 mg / kg after acid bamboo shoots are fermented by the strain for 7 days; after the brine is fermented for 4 to 5 days, the content of p-cresol is 15.5 to 22 mg / kg; the content of p-cresol in the fish dices fermented for 5 days is 5-6 mg / kg, the fermentation effect reaches the content of p-cresol obtained after fermentation of an existing natural fermentation method for 1 month, and the fermentation speed is effectively shortened.

The invention discloses application of lactobacillus fermentum E1 in preparation of fat-reducing and weight-losing products, and belongs to the technical field of microorganisms. The lactobacillus fermentum E1 disclosed by the invention can remarkably promote fat metabolism in the zebra fish body in vivo, can remarkably reduce the content of triglyceride and total cholesterol in the hyperlipidemia zebra fish body, and has the potential of being applied to losing weight and regulating the blood fat level; theoretical reference and guidance basis are provided for developing probiotic preparations for treating or preventing obesity and hyperlipidemia by using the lactobacillus fermentum E1.

The application discloses a stomach-derived limosilactobacillus fermentum LAF-09 and application thereof, and the preservation number of the stomach-derived limosilactobacillus fermentum LAF-09 is GDMCC No. 65656. The inventor of the application screens a stomach-derived limosilactobacillus fermentum LAF-09 from stomach mucosa tissue of healthy people, the strain has strong acid resistance and high proliferation capacity in the stomach, has strong adhesion properties to stomach epithelial GSE-1 cells, can be effectively applied to prevent and treat gastric ulcers caused by indomethacin, and has no obvious side effects.

The invention discloses a composition with effects of reducing weight and reducing blood fat and application of the composition, and belongs to the technical field of biology. The composition comprises 30%-80% of a probiotic compound and 20%-70% of a raw material containing glycerol glycolipid, the probiotic compound takes lactobacillus reuteri as an essential component, and further comprises any one or more of bifidobacterium, lactobacillus fermentum, lactobacillus fermentum, lactobacillus acidophilus, lactobacillus casei, lactobacillus rhamnosus, lactobacillus brevis and bacillus coagulans. According to the invention, the specific probiotic compound is screened and compounded with the raw materials rich in glyceroglycolipid, and the synergistic effect of the probiotic compound and the raw materials is utilized to improve the weight-reducing and blood fat-reducing effects. According to the composition developed by the invention, the problem of resource waste caused by the fact that raw materials such as nannochloropsis oculata oil rich in glyceroglycolipid cannot be effectively absorbed in the prior art is solved, the nutritional effect of the composition is improved, and the composition has a wide application prospect in the fields of food, health care products and medicines.

The application belongs to the technical field of microbial fermentation, and particularly relates to a fermentation product of fruit and vegetable wastes and corn stalks and application thereof. The fermentation product is obtained by inoculating Aspergillus oryzae SLY-M-15 fermentation product and Saccharomyces cerevisiae into a first mixture containing crushed and air-dried corn stalks and fruit and vegetable wastes, performing aerobic fermentation, adding a second mixture containing crushed and air-dried corn stalks and fruit and vegetable wastes and corn cob, performing secondary aerobic fermentation, inoculating Lactobacillus casei, Lactobacillus acidophilus and Lactobacillus fermentum, and performing anaerobic fermentation. The obtained fermentation product is applied to animal feed, can improve the average daily weight gain of mutton sheep, reduce the feed conversion ratio, improve the tenderness and cooked meat rate of mutton, and increase the content of n-3 polyunsaturated fatty acids.

This invention discloses a combined microbial agent and its application in combating clubroot disease, belonging to the field of microbial pesticide technology. By weight percentage, the combined microbial agent comprises 20%-30% Bacillus subtilis, 15%-25% Bacillus amyloliquefaciens, 10%-25% Lactobacillus fermentum, 10%-20% Isaac's yeast of Orientia, and 10%-20% Actinobacillus sieboldii. There is a synergistic effect among the strains, resulting in a significant effect on the control of clubroot disease, superior to conventional pesticides such as chlorothalonil. Using the combined microbial agent provided by this invention to control clubroot disease can reduce the amount of chemical pesticides used, exhibiting environmental friendliness and high efficiency. Furthermore, field trials have shown that treatment with the combined microbial agent of this invention reduces the incidence of clubroot disease in cruciferous crops while significantly increasing crop yield, resulting in economic benefits. The combined microbial agent of this invention is suitable for practical production and easy to promote and apply.

The present invention relates to a synergistic bioactive composition for women's health, and more particularly to compositions comprising Lactobacilli and Lactoferrin for supporting vaginal health. The invention specifically provides a composition comprising Lactobacillus crispatus, Lactobacillus jensenii, Lactobacillus fermentum, and Lactoferrin, formulated with one or more pharmaceutically acceptable excipients. The invention further pertains to compositions and kits intended for promoting a balanced vaginal microbiota, maintaining healthy vaginal conditions, and supporting overall vaginal ecosystem stability.

The invention relates to the technical field of probiotics, and discloses a probiotic preparation for improving abnormal bile diseases of newborns. According to the probiotic preparation provided by the invention, lactobacillus fermentum CECT5716, bifidobacterium breve M-16V and bifidobacterium longum subsp. Longum BB536 in the list of strains for infants are selected as a product formula, and the probiotic preparation can be used for effectively improving abnormal bile diseases of newborns, including jaundice, hyperbilirubinemia and cholestasis, and can be used for preventing and treating the abnormal bile diseases of the newborns, such as jaundice, hyperbilirubinemia and cholestasis. The problems that in the prior art, safety is insufficient, pertinence is insufficient, and clinical requirements of complex illness conditions cannot be met are effectively solved.

The application belongs to the technical field of microorganisms, and discloses Lactobacillus fermentum (L.f) LFSJ001 and application thereof in preparation of products for preventing or treating ulcerative colitis. The Lactobacillus fermentum LFSJ001 is independently isolated and identified. It is found through the use of DSS to construct a mouse colitis model that the Lactobacillus fermentum LFSJ001 combined with 5-Aminosalicylic Acid (5-ASA) can enhance the improvement of 5-ASA on the symptoms of colitis, including multiple indexes such as disease activity index, colon injury, and spleen index. It can be seen that the Lactobacillus fermentum LFSJ001 can be used as a combined drug of 5-ASA, enhance the efficacy of 5-ASA, inhibit the occurrence and development of ulcerative colitis, and improve the intestinal barrier. The application provides a new diagnosis and treatment direction and strategy for the prevention and treatment of ulcerative colitis.

The present invention relates to a composition for preventing, ameliorating or treating cognitive dysfunction or Alzheimer's disease comprising, as an active ingredient, Lactobacillus fermentum SRK414 strain which has accession number KCTC13687BP. The strain of the present invention is excellent in reducing barrier permeability and reducing amyloid beta and tau proteins and has an excellent effect in improving cognitive function, and accordingly, the strain can be useful for a food and therapeutic agent for same purposes.

The application provides a compound probiotic postbiotic fermentation product for relieving intestinal damage caused by microplastics, and an application thereof. The compound probiotic postbiotic fermentation product is prepared from fermentation of Lactobacillus plantarum HNU082, Lactobacillus fermentum HNU312 and Lactobacillus paracasei HNU502. The postbiotic prepared by the application can relieve inflammation induced by polystyrene, relieve reduction of Occludin and Claudin-1 expression induced by polystyrene, and relieve reduction of the number of colon goblet cells induced by polystyrene. Therefore, the postbiotic prepared by the application can relieve damage to the intestinal barrier of an organism caused by microplastics.

The application discloses application of Limosilactobacillus fermentum CET5716 exopolysaccharide (EPS) in preparation of a drug or a functional food for preventing or improving chronic kidney disease induced by adenine, and the Limosilactobacillus fermentum CET5716 exopolysaccharide (EPS) is obtained through centrifugation, protein removal, alcohol precipitation, dialysis, dextran gel separation and purification, and freeze-drying of fermentation liquor of Limosilactobacillus fermentum CECT5716. Animal in-vivo experiments show that the Limosilactobacillus fermentum CET5716 exopolysaccharide can improve chronic kidney disease of mice induced by adenine through gavage.