1733 results about "Glutathione" patented technology

The invention discloses an anti-aging composition containing ergothioneine, glutathione and fish collagen, relates to the technical field of anti-aging compositions, and aims to solve the technical problems that a traditional anti-aging composition lacks attention to the aspects of cell energy metabolism, barrier repair and the like, and the overall skin improvement effect is not obvious. The ergothioneine is used as a natural antioxidant, and the content of the ergothioneine is 0.01-5wt%; the ergothioneine participates in a synergistic effect of an intracellular antioxidant enzyme system, so that tyrosinase activity is effectively inhibited, melanogenesis is reduced, and DNA and protein are protected from oxidation damage; in a simulated strong ultraviolet environment, compared with skin cells not using the ergothioneine-containing product, the content of free radicals in the cells is reduced by 30% after the ergothioneine-containing product is used; the ergothioneine can also regulate a cell signal channel, promote cell metabolism and maintain normal growth of cells. The mask has the advantages of accelerating skin metabolism and improving the self-repairing ability of the skin.

The invention discloses a preparation method and application of a zinc-manganese bimetal organic framework derived composite nano-enzyme, and belongs to the technical field of nano-materials. Zinc-manganese bimetal organic framework nanoparticles prepared by reacting ZIF-8 with manganese (III) acetylacetonate are used as a template, and are calcined at different temperatures in a tubular furnace to obtain zinc-manganese bimetal organic framework derivative composite nano-enzymes with different morphologies. The nano enzyme has peroxidase-like activity, glutathione peroxidase-like activity and catalase-like activity, and has a wide application prospect in anti-tumor treatment.

The invention relates to a composite hydrogel dressing for promoting wound healing as well as preparation and application thereof, and belongs to the technical field of biological medicines. The hydrogel disclosed by the invention is prepared by mixing gelatin, oxidized dextran and metal ion modified metal-organic framework compounds (MOF-Ag and MOF-Cu). In the mixed solution, amino groups in the gelatin and aldehyde groups of the oxidized dextran are subjected to a Schiff base cross-linking reaction, and the composite hydrogel is formed after standing. The loaded MOF-Ag has antibacterial and anti-biofilm effects on staphylococcus aureus and escherichia coli, and the loaded MOF-Cu has similar superoxide dismutase activity and similar glutathione peroxidase activity, so that excessive active oxygen can be removed, and inflammatory response can be relieved. The obtained hydrogel has good biocompatibility, proper mechanical properties and biological adhesion, stable rheological properties, excellent antibacterial and anti-inflammatory functions and a certain hemostatic function.

The invention provides a preparation method of medicinal liquor and application of the medicinal liquor in preparation of antioxidant and anti-aging products, and relates to the technical field of medicinal preparations. The preparation method comprises the following steps: (1) crushing; (2) extracting; (3) standing and separating; and (4) freezing and filtering to obtain the medicinal liquor. The medicinal liquor is obtained by extracting the traditional Chinese medicine components through the steps of crushing in batches, mixing and extracting, standing, freezing and separating the supernate and the suspension in different separation modes, and the medicinal liquor can better improve the space exploration capability of a mouse aged due to D-galactose, can reduce malondialdehyde MDA, and can improve the immunity of the mouse. The activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) is improved, and the obvious anti-oxidation and anti-aging effects are achieved.

The invention relates to the field of biomedical materials, and particularly discloses a manganese dioxide nano-enzyme for treating inflammatory bowel disease as well as a preparation method and application of the manganese dioxide nano-enzyme. The preparation method of the manganese dioxide nano-enzyme comprises the following steps: uniformly mixing an organic solution containing manganese nitrate hexahydrate with a montmorillonite aqueous solution to obtain a mixed reaction solution; the pH value of the mixed reaction solution is adjusted to be under the alkaline condition through alkali liquor, a synthetic reaction is carried out, then centrifugation and washing are carried out, the montmorillonite-loaded manganese dioxide nano-enzyme is obtained, and the manganese dioxide nano-enzyme is of a lamellar structure with the length being 20-400 nm. The manganese dioxide nano enzyme has excellent activity similar to superoxide dismutase, catalase and glutathione peroxidase; meanwhile, the compound also has the capability of removing various free radicals such as DPPH and ABTS, can be used for treating the inflammatory bowel disease, and has a wide application prospect.

The invention relates to the field of biomedical materials, in particular to a composite hydrogel microsphere with excellent cell adhesion performance and a preparation method and application thereof, and the preparation method comprises the following steps: (1) modifying silk fibroin with glutathione to obtain sulfydryl modified silk fibroin SF-GSH; (2) adopting methacrylic anhydride as a double-bond introduction reagent, introducing a methacrylate group on hyaluronic acid and forming a carbon-carbon double bond to obtain double-bond modified hyaluronic acid HA-MA; (3) mixing the sulfydryl modified silk fibroin SF-GSH with double bond modified hyaluronic acid HA-MA, and adding lactobionic acid to prepare a composite hydrogel microsphere precursor solution; and (4) preparing the composite hydrogel microsphere precursor solution into the composite hydrogel microsphere containing lactobionic acid. Compared with single hyaluronic acid hydrogel, the composite hydrogel microspheres prepared by the preparation method provided by the invention have good biocompatibility.

Provided are a whitening and freckle-removing composition, a gel, a preparation method therefor, and a use thereof, relating to the technical field of skin-care products. The whitening and freckle-removing composition comprises tranexamic acid, nicotinamide, 4-butylresorcinol, licorice extract, 3-o-ethyl ascorbic acid, and glutathione. For the whitening composition, by inhibiting the activity of tyrosinase, blocking the transfer of melanin from melanocytes to keratinocytes, and inhibiting the formation and accumulation of melanin by means of multiple mechanisms including oxidation resistance, and by means of the synergistic action of all components, cosmetic benefits of whitening and freckle-removing are achieved. The whitening and freckle-removing gel has a simple preparation method, has a mild and non-sensitizing formula, is safe and non-irritating, and is suitable for large-scale production and application.

The invention provides an application of an active peptide in preparation of a medicine for preventing and treating ALD (Atomic Lymphosis Disease). The amino acid sequence of the active peptide is shown as SEQ ID NO: 1, the active peptide can effectively relieve damage of ethyl alcohol to liver cells, increase of glutamic-pyruvic transaminase activity, lactic dehydrogenase activity and malondialdehyde content and decrease of glutathione content caused by ethyl alcohol can be remarkably inhibited, then the preventing and treating effect on ALD is achieved, and the active peptide is suitable for preparing drugs for preventing and treating ALD and has a good application prospect. Meanwhile, an active peptide resource library with ALD prevention and treatment effects is also enriched.

The invention discloses bacillus subtilis ZX-5 for promoting growth of gleditsia sinensis under salt stress and application of the bacillus subtilis ZX-5, and relates to the technical field of microorganisms. The invention discloses bacillus subtilis ZX-5 for promoting growth of gleditsia sinensis under salt stress, and the bacillus subtilis ZX-5 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.31648. The result of the embodiment shows that the bacillus subtilis ZX-5 promotes the ground diameter growth rate and seedling height growth rate of the gleditsia sinensis to be improved under salt stress, and promotes the total root length and root tip number of the gleditsia sinensis root system to be increased; the surface area of the gleditsia sinensis root is increased under the stress of 150mM salt. The activities of Chinese honey locust superoxide dismutase, peroxidase, catalase and ascorbic acid peroxidase are promoted to be enhanced under the stress of salt; the contents of ascorbic acid and reduced glutathione in the gleditsia sinensis are promoted to be increased under the stress of salt; the content increase of soluble protein, soluble sugar and free proline in the gleditsia sinensis is promoted.

The invention discloses a drug-loaded liposome nano-particle, a preparation method thereof and application of the drug-loaded liposome nano-particle in treatment of brain glioma, and belongs to the technical field of biological nano-medicines. The invention provides a novel copper phthalocyanine derivative and catalase co-loaded lipidosome nanoparticle and application of the novel copper phthalocyanine derivative and catalase co-loaded lipidosome nanoparticle to treatment of brain glioma on the cellular level. The nano-particles are obtained by wrapping copper phthalocyanine derivatives and catalase with lipidosome prepared by a microfluidic method. The co-drug-loaded liposome can catalyze excessive H2O2 in brain glioma to generate oxygen, and O2 is continuously supplied to photodynamic therapy (PDT) to generate active oxygen; meanwhile, Cu < 2 + > can deplete glutathione and improve the oxidative stress level in the glioma, the reduction product Cu < + > can convert H2O2 into hydroxyl radicals with higher toxicity through a Fenton-like reaction, oxidative stress injury is doubly amplified, cascade cooperation of PDT and chemical dynamic therapy (CDT) is achieved, and the treatment effect on the glioma is remarkably enhanced.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to the daily gain character of a pig. The SNP molecular marker is a Ggt (Glutathione Ggt) located at the position of 160773437bp of a first chromosome of a pig genome Ensembl Sscrofa11.1 version; a mutation. Through the SNP molecular marker, the SNP molecular marker can be applied to breeding of the daily weight gain character of the Duroc long commercial pigs, so that the daily weight gain character of the Duroc long commercial pigs is improved, the growth performance and the slaughter weight of the Duroc long commercial pigs are improved, and the economic value and the economic benefit of the Duroc long commercial pigs are improved. And the variety of the Duroc long commercial pigs can be improved, the daily gain character of the variety is further improved, improved variety breeding is performed, and the market competitiveness of the Duroc long commercial pigs is improved. In addition, due to the fact that the Duroc long hybrid population is adopted as a research object, the Duroc long hybrid population can also serve as a reference population of a male parent Duroc purebred population, and reference and application values are improved for breeding of male parent Duroc boars in daily gain.

The invention relates to a culture medium and a culture method of persistent DC-CIK cells and application of the culture medium and the culture method, and belongs to the technical field of cell culture. The invention provides a culture medium for persistent DC-CIK cells, which takes a serum-free culture medium as a solvent and comprises a recombinant human granulocyte macrophage colony stimulating factor, a recombinant human interleukin-12, a recombinant human tumor necrosis factor-alpha, a recombinant human interferon-gamma, L-cysteine, glutathione, ginseng polysaccharide and streptomycin sulfate. According to the culture medium and the culture method of the persistent DC-CIK cells, proliferation and growth of the DC-CIK cells can be promoted, and very high tumor killing activity of the DC-CIK cells can be maintained for a long time.

The invention discloses a preparation method and application of a manganese-based amino acid coordination polymer, and belongs to the technical field of nano-enzymes and nano-drugs. Divalent manganese salt, histidine and other amino acids are subjected to a co-precipitation reaction to prepare the manganese-based amino acid coordination polymer, and the polymer is of a nanoparticle structure, small in size and high in stability, has enzyme-like activity, catalyzes hydrogen peroxide to generate high-toxicity hydroxyl free radicals and is used for specifically killing tumor cells. The catalyst has the capability of catalytically generating singlet oxygen in the presence of hydrogen peroxide. Meanwhile, the manganese-based amino acid complex has glutathione oxidase-like activity, consumes glutathione to overcome the resistance of tumors to oxidative stress, improves the chemical kinetic curative effect, and has anti-tumor application potential.

The invention provides a polypeptide and application thereof in preparation of a medicine for preventing and / or treating alcoholic liver injury. The polypeptide is safe and free of side effects, can remarkably reduce the content of malondialdehyde (MDA), improve the activity of superoxide dismutase (SOD), improve the content of glutathione (GSH), reduce the activity of glutamic-pyruvic transaminase (ALT), reduce the activity of aspartate transaminase (AST) and reduce the activity of lactic dehydrogenase (LDH), and is very suitable for preparing the medicine for preventing and / or treating the alcoholic liver injury.

The invention discloses parabacteroides faecium capable of relieving senescence and application of the parabacteroides faecium, and belongs to the technical field of microorganisms and the technical field of medicines. Animal experiments prove that Parabacteroides vervae CCFM1428 has the effects of improving the weakness index of a mouse, increasing peroxide dismutase in the liver of the mouse, reducing malonaldehyde, increasing the level of glutathione peroxidase (GSH-px), increasing the level of catalase, reducing an inflammatory factor IL-6, reducing an inflammatory factor TNF-alpha and increasing the level of acetylcholine in the brain of the mouse, and has the effects of improving the weakness index of the mouse, increasing the liver peroxide dismutase of the mouse, reducing malonaldehyde, increasing the level of glutathione peroxidase (GSH-px) and the level of catalase. The gripping force of the mouse is improved. The strain disclosed by the invention has a huge application prospect in preparation of products for relieving senescence.

The invention discloses a preparation method of efficient antioxidant sturgeon protein peptide. The preparation method comprises the following steps: S1, extracting a sturgeon skin crude protein solution; s2, extracting sturgeon swimming bladder crude protein; s3, extracting crude protein liquid of the sturgeon meat; s4, performing mixed enzymolysis; and S5, carrying out enzyme deactivation treatment on the obtained enzymatic hydrolysate to obtain enzymatic hydrolysate, and sequentially carrying out ultrafiltration and vacuum drying on the enzymatic hydrolysate to obtain the efficient antioxidant sturgeon protein peptide. Proteins extracted from different parts of sturgeons are mixed according to a specific proportion and then subjected to enzymolysis, the added neutral protease and papain can achieve a synergistic effect, protein is hydrolyzed more comprehensively, small molecule peptides with specific functions are generated, meanwhile, glutathione added during enzymolysis can protect sulfydryl of enzymes, and the enzymolysis effect of the sturgeons is improved. The enzyme activity is maintained, so that the finally obtained sturgeon protein peptide has good oxidation resistance.

The invention discloses a macrocyclic covalent organic polymer material for inhibiting a pi-pi stacking effect and application of the macrocyclic covalent organic polymer material, and belongs to the technical field of biological medicines. The preparation method comprises the following steps: adding 5, 10, 15, 20-tetra (4-ethynylphenyl) copper porphyrin and a macrocyclic monomer into an organic solvent in a protective atmosphere, and carrying out solvothermal reaction in the presence of a catalyst and alkali to obtain the macrocyclic covalent organic polymer material. According to the invention, 5, 10, 15, 20-tetra (4-ethynylphenyl) copper porphyrin and a macrocyclic monomer form a porous polymer MCP-CU-POP, the material utilizes the photo-thermal capability to combine the effects of simulated photo-thermal (PTT), photodynamic (PDT), peroxidase (PODs) and glutathione peroxidase (GSH-Px) to perform synergistic antibiosis, and the inherent characteristics of an infected microenvironment can also be utilized to induce death of bacteria, so that the antibacterial property of the material is greatly improved, and the antibacterial property of the material is greatly improved. And the microenvironment of the bacterial infection part can be adjusted, so that the recovery of the bacterial infection wound can be obviously accelerated.

The invention belongs to the field of synthesis of nano materials, and particularly relates to a core-shell structure gold nano star and a preparation method and application thereof, and the core-shell structure gold nano star comprises a first gold nano star containing a metal sulfide shell layer and a second gold nano star containing a metal oxide shell layer. The preparation method comprises the following steps: firstly preparing a gold nano star core solution, and then respectively adding different precursors to prepare two core-shell structure gold nano stars. The method has the advantages of simple synthesis conditions and non-toxic reagents. The core-shell structure gold nanostars are used for preparing a detection solution based on a buffer solution, a color developing agent and an oxidizing agent, the detection solution reacts with antioxidants, a data matrix is constructed by measuring absorbance changes and is analyzed, single, binary and ternary combinations of various antioxidants such as ascorbic acid and glutathione can be accurately distinguished, and the detection accuracy is improved. The application prospect in the fields of biomolecules and food detection is good.

The invention discloses a medicinal and edible anti-alcoholism composition and a preparation method thereof, and relates to the crossing field of biotechnology and food and medicine, the composition comprises the following components: dried orange peel powder, vitamin C, glutathione, phellinus igniarius extract, polygonatum sibiricum extract, oriental wormwood extract, isatis root extract, schisandra chinensis extract, ginseng powder, lucid ganoderma extract and licorice extract. Silymarin, puerarin, white tomato powder, watermelon powder, fucoidin, tomato powder, mung bean powder, celery powder, turmeric powder, hawthorn fruit powder, honey powder, corn peptide, soybean peptide powder, xanthan gum and stevioside are added. The composition provided by the invention has the effect of increasing the content of ethanol dehydrogenase and acetaldehyde dehydrogenase in blood after drinking, and has the capability of promoting alcohol metabolism.

The invention provides a preparation method of NMN nano microspheres with high stability, and belongs to the technical field of NMN. The preparation method comprises the steps of NMN pretreatment, primary microsphere preparation, functional microsphere preparation and mixing. The preparation method of the primary microspheres comprises the following steps: adding epsilon-polylysine and glutathione into an ethanol solution, uniformly stirring, adding 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide, and stirring for 28-32 minutes to obtain a mixed solution; adding the pretreated NMN solution into the chitosan solution, then adding the mixed solution, and carrying out heat preservation at 44-50 DEG C for 2.8-3.2 h to obtain primary microspheres; the NMN nano-microsphere prepared by the method disclosed by the invention is good in stability and good in slow release performance.

The invention discloses organic nano composite hydrogel as well as a preparation method and application of the organic nano composite hydrogel, and belongs to the technical field of biological medicine delivery. The polysaccharide-based nano prodrug in the composite hydrogel can be subjected to surface protonation under the stimulation of a tumor extracellular slightly acidic environment, so that cellular uptake is promoted, low-pH / high-concentration glutathione in tumor cells is responded, intracellular aggregation and controllable release of the nano drug are realized, the retention time of the drug in the cells is prolonged, and the bioavailability of the drug is improved. The technical effect of killing tumor cells through combined chemotherapy is achieved. According to the preparation method disclosed by the invention, the release of the polysaccharide-based nano prodrug from the hydrogel and the tissue infiltration capacity are accelerated by adopting fluorinated orthoester, the infiltration of the polysaccharide-based nano prodrug to cells is increased, and then the drug-loaded compound is encapsulated by using gellan gum, so that the slow-release effect of the polysaccharide-based nano prodrug is enhanced; therefore, the efficient enrichment of the medicine at the tumor part is realized.

According to the application of the BnaGSTU11 gene in regulating and controlling the sclerotinia rot resistance of brassica napus, the amino acid sequence is as shown in SEQ ID NO: 2. According to the invention, a gene BnaGSTU11 for coding glutathione S-transferase is separated and cloned from brassica napus, and protease coded by the gene can enhance the sclerotinia rot resistance of the brassica napus by removing active oxygen and free radicals. The discovery of the sclerotiniose-resistant gene BnaGSTU11 provides a new genetic resource for cultivating a new sclerotiniose-resistant rape germplasm, and also provides a new thought for mining sclerotiniose-resistant genes of other crops.

The invention discloses an active peptide with effects of resisting oxidation and protecting alcoholic liver injury and application of the active peptide. According to the invention, walnut protein hydrolysate is taken as a raw material, and two novel peptide fragments HGEPGQQQR and VAPFPEVFGK with high antioxidant activity are obtained through screening. The two active peptides show good antioxidant activity on the cellular level, and the EC50 values of the two active peptides are 0.0120 mg / mL and 0.0068 mg / mL respectively, which are both superior to 0.0122 mg / mL of positive control glutathione. In an ethanol injury model, the survival rates of cells subjected to ethanol injury treatment can be respectively increased from 47.35% to 62.69% and 57.05% by the two antioxidant peptides. The results show that the walnut protein source antioxidant peptides HGEPGQQQR and VAPFPEVFGK can be developed and applied as a potential antioxidant and a liver protection active component.

The invention provides a microneedle preparation for co-delivering photo-thermal nano-enzyme and elemene as well as a preparation method and application of the microneedle preparation. The preparation method comprises the following steps: firstly, preparing a novel photo-thermal nano enzyme Au (at) MoS2 through a two-step water phase synthesis strategy; benefited from the synergistic effect of the hybrid material, the nano-enzyme shows significantly enhanced near-infrared (NIR) photothermal effect, peroxidase-like (POD) activity and glutathione-like peroxidase (GSHOx) activity, and can effectively remodel the tumor microenvironment and destroy the redox steady state. Furthermore, photo-thermal nano enzyme, beta-ELE and hyaluronic acid are used as raw materials, and a novel soluble microneedle preparation is successfully prepared through a template method. The novel composite microneedle is in a sharp rectangular pyramid shape, growth of melanoma can be effectively inhibited through percutaneous delivery of photo-thermal nano-enzyme and beta-ELE, remarkable systemic toxicity is avoided, and an innovative strategy is provided for non-invasive, efficient and safe melanoma combined treatment.

The invention discloses a pesticide residue scavenging agent. The feed additive is prepared from the following components in parts by weight: 90-110 parts of white hyacinth beans, 9-11 parts of papain, 90-110 parts of liquorice, 45-55 parts of folium isatidis, 25-35 parts of perilla leaves, 25-35 parts of fructus forsythiae, 25-35 parts of honeysuckle, 15-25 parts of rhizoma smilacis glabrae, 15-25 parts of ginkgo leaves, 4-6 parts of soybean protease, 9-11 parts of glutathione, 5-7 parts of amino-oligosaccharin, 9-11 parts of amylase and 7-9 parts of walnut peptide. The invention also discloses a preparation method of the pesticide residue scavenger. The method has the following outstanding effects: the method is easy to operate, low in cost, popular in price and remarkable in effect, and can reach the national safety agricultural product indexes.

The invention belongs to the technical field of nuclear magnetic resonance spectrums, and particularly relates to a preparation method of a responsive multi-component material and application of the responsive multi-component material in 129Xe / 1H dual-mode signal change. According to the method, the Fe3O4 (at) SiO2 nanoparticles are used as a precursor, KMnO4 is used as a raw material, the Fe3O4 (at) SiO2 (at) MnO2 nanoparticles are synthesized, and the synthesized nanoparticles are complete in morphology and uniform in size. A transverse relaxation rate 1 / T2 signal of protons of the multi-component material obtained by the strategy is quenched, a dissolved state signal of 129Xe is basically unchanged, but after the reaction with 10mM glutathione, the 1 / T2 signal is recovered, the dissolved state signal of 129Xe is obviously reduced, the change of a 129Xe / 1H double nuclear magnetic resonance signal is realized, and the change of the 129Xe / 1H double nuclear magnetic resonance signal is realized. And the method has certain significance for enriching the application of the hyperpolarized 129Xe magnetic resonance probe and the development of a multi-mode magnetic resonance imaging nano platform.

The present invention discloses a complete medium for culturing DF-1 cells, including DMEM basal medium 13.7g / L, insulin, non-essential amino acids, transferrin, glutathione, ascorbyl phosphate, sodium selenite, ethanolamine, hydrocortisone, and newborn calf serum. Wherein non-essential amino acids include glycine, alanine, proline, serine, and asparagine. A complete medium for culturing DF-1 cells provided by the present invention can well promote the proliferation of DF-1 cells, can achieve the same proliferation effect as high-concentration fetal bovine serum, and is suitable for the adherent culture of DF-1 cells; using low-concentration newborn calf serum, it is possible to greatly avoid the batch differences, unstable culture results, cytotoxicity, and high risk of carrying pathogens brought by the culture medium with high-concentration serum, narrow application range, expensive fetal bovine serum, and high culture cost; the components are simple, the culture medium preparation process is short in time consumption, the culture efficiency is high, the repeatability is good, and it is suitable for batch production.

The invention provides a biological combined preparation for allergic rhinitis and a preparation method of the biological combined preparation. The preparation comprises antibacterial polysaccharide, a centipeda minima extract, anti-allergic probiotics, an active polypeptide compound, sodium hyaluronate, an immunomodulator, a penetration enhancer, a biological adhesive and the like. Wherein the antibacterial polysaccharide can be selected from various natural polysaccharides; the centipeda minima extract is obtained by a specific ethanol reflux method; the antiallergic probiotics comprise lactobacillus acidophilus, lactobacillus rhamnosus and lactobacillus reuteri; the active polypeptide compound consists of thymosin, glutathione and osteogenic peptide. The preparation method comprises the following steps: sequentially mixing the components, carrying out high-pressure homogenization treatment, carrying out ultrasonic treatment, adding probiotics, stirring, intercepting with an ultrafiltration membrane, freeze-drying and the like. The preparation disclosed by the invention can form a uniform nano-scale particle suspension, so that the release rate and the absorption efficiency of the medicine are further improved, and an efficient, safe and convenient novel method is provided for treating the allergic rhinitis.

The invention discloses a glutathione bifunctional synthetase mutant with improved catalytic activity and application, and belongs to the field of gene engineering and fermentation engineering. A series of mutants with improved enzyme activity are obtained by carrying out site-specific combinatorial mutation on a site 61, a site 136, a site 485, a site 498 and a site 722 of the glutathione bifunctional synthetase GshFst, the specific enzyme activity reaches 13.58 U.mg <-1 >-27.34 U.mg <-1 >, the half-life period at 37 DEG C reaches 195-320 min, and the enzyme activity and the thermal stability are remarkably improved compared with those of a wild type. The enzyme mutant provided by the invention has a wide application prospect in the production of glutathione and the construction of genetically engineered microorganisms for producing glutathione.

The invention relates to the field of biological functional materials, and discloses a preparation method and application of a Cu-doped W18O49 nano-enzyme with near-infrared two-region photoresponse. The specific method is as follows: a solvothermal method is adopted, the Cu-doped W18O49 (xCu-W18O49) nano-enzyme is prepared by regulating and controlling the molar doping amount of a Cu element, taking tungsten chloride as a tungsten source, taking copper chloride dihydrate as a copper source and taking n-propyl alcohol as a reaction solvent. The crystallinity of the nano enzyme is good, and the nano enzyme shows remarkable glutathione oxidase (GSHOx) activity in a tumor microenvironment. Compared with W18O49, Cu doping enables enzyme activity to be remarkably improved, the problem of low activity generally existing in current cancer treatment nano-enzymes is effectively solved, and meanwhile the nano-enzyme has application potential in the aspect of CT imaging.