112 results about "Glutathione" patented technology

The application discloses a photosensitizer conjugate for targeted treatment of tongue squamous cell carcinoma and a preparation method and application thereof, and belongs to the technical field of biological medicines. A novel PROTAC-PDT conjugate Gef-PRO-SS-TPE is synthesized, and the compound connects a PROTAC molecule Gef-PRO targeting EGFR and an AIE photosensitizer TPE derivative through a breakable disulfide bond. On one hand, Gef-PRO can specifically degrade EGFR and block tumor cell proliferation signals; on the other hand, the TPE derivative can generate ROS under light conditions and induce cell apoptosis. More importantly, the disulfide bond can be broken in response to high concentrations of glutathione (GSH) in tumor cells, realizing controllable release of Gef-PRO and TPE molecules, so as to exert a synergistic anti-tumor effect.

This invention discloses a NK cell culture kit, its culture method, and its application. The kit includes an amplification medium, a high-efficiency induction medium, an NK-A coating solution, an NK-B mixture, and an NK-C mixture. The amplification medium contains basal medium, NAD+, human serum albumin, transferrin, β-glucan, glutathione, β-mercaptoethanol, and linoleic acid. The high-efficiency induction medium, in addition to the amplification medium components, contains soybean peptides, nicotinamide, inulin, and N-acetyl-L-cysteine. The NK-A coating solution contains heparin sodium, monoclonal antibodies, and antibodies. The NK-B mixture contains Inbakicept, IL-2, and IL-15. The NK-C mixture contains linolenic acid, IL-2, and IL-18. The Inbakicept factor in the kit can activate NK cells and enhance their cytotoxicity; linolenic acid can inhibit T cell proliferation, increase NK cell purity, and enhance cell efficacy.

The application relates to the field of biological products, in particular to application and application method of glutathione in medical devices or cosmetics, and a medical device / cosmetic containing glutathione, and provides application of glutathione in specific medical devices (such as products for facial superficial dermis injection, products for wound care) and various cosmetics, wherein the amount of glutathione is 0.1-3 wt%, the glutathione is used in combination with sodium hyaluronate at a mass ratio of (0.1-3):(0.6-3), and specific antioxidant components (such as vitamin C, vitamin E, etc.) and chelating agents can be further compounded to form a synergistically stable composite system. The application effectively improves the storage stability of glutathione in the product, and enables the glutathione to be adapted to various application scenes such as injection, wound care and skin care, thereby providing a reliable technical scheme for industrial application of glutathione in the fields of medical devices and cosmetics.

This invention relates to an isosorbide dinitrate pharmaceutical composition injection and its preparation method. In addition to isosorbide dinitrate, the injection formulation also contains meglumine and glutathione. The preferred specifications are 5ml:5mg and 10ml:10mg. The isosorbide dinitrate injection prepared according to the formulation and process of this invention has the characteristics of high excipient safety, simple process, extremely high quality stability and good safety, which is significantly better than the prior art.

A method for separately quantifying N-acetylcysteine and glutathione in brain tissue includes administering N-acetylcysteine to a subject, acquiring a first magnetic resonance spectroscopy signal from a region of brain tissue of the subject, the first magnetic resonance spectroscopy signal comprising a cysteine β-proton signal, acquiring a second magnetic resonance spectroscopy signal from the region of brain tissue, the second magnetic resonance spectroscopy signal comprising an N-acetyl proton signal, and determining a concentration of N-acetylcysteine in the region of brain tissue based on the N-acetyl proton signal and determining a concentration of glutathione in the region of brain tissue based on a difference between the cysteine β-proton signal and the N-acetyl proton signal.

The application provides a kind of mitochondrial function optimization additive for cell culture, preparation method and application, belongs to cell culture technical field.The additive includes core active ingredient, auxiliary component and carrier, and the core active ingredient includes D-Arg-Cha-Lys-Phe-NH2 Polypeptide, ubiquinone-10 phosphate, reduced glutathione and alpha-lipoic acid.The application forms a multi-target core active system through the synergy of the four, realizes the triple synergistic protection of stabilizing mitochondrial cardiolipin, maintaining mitochondrial morphology and membrane potential, and reducing mitochondrial ROS, which can significantly improve cell culture quality.When applied, the mitochondrial cardiolipin content can be increased by more than 50%, the mitochondrial abnormality ratio can be reduced to less than 12%, the membrane potential positive cell ratio can be increased to more than 86%, the mitochondrial ROS level can be reduced by more than 40%, and the cell proliferation rate can be increased by more than 25%, which is suitable for in vitro culture of various eukaryotic cells, and the preparation process is simple and has no cytotoxicity.

The application discloses application of a cerium monatomic functional material in preparation of a tumor radiotherapy sensitizer. SA In the material, cerium is dispersed on a nitrogen-doped carbon carrier in a monatomic form and forms a stable coordination structure with N / O. The application determines the structure-activity relationship of the unique physical and chemical structure and multiple enzyme activities, verifies the radiotherapy sensitization effect and biological safety in and out of the body. The material has multiple enzyme activities such as simulating peroxidase and glutathione oxidase, can efficiently catalyze ROS and consume GSH, and enhances DNA damage and apoptosis induced by ionizing radiation. In vitro clone formation experiments show that the radiotherapy sensitization ratio reaches 1.38, which is better than that of CeO2 nano clusters (1.17). In vivo experiments on tumor-bearing mice prove that the material combined with radiotherapy can significantly inhibit tumor growth, prolong survival time, and has good biocompatibility. The application provides a new strategy of high efficiency and low toxicity for tumor radiotherapy sensitization, and has a good clinical application prospect.

This invention discloses a method for eliminating moss growth on macadamia nut leaves, relating to the field of agricultural planting technology. The specific method involves spraying a moss-removing agent onto the macadamia nut leaves during the moss occurrence period, applying moss inhibitor I to the main stem, and applying moss inhibitor II to the soil surface around the main stem. The moss-removing agent comprises the following raw materials in parts by weight: 40-50 parts of allicin, 15-24 parts of urea, 5-18 parts of calcium propionate, 20-27 parts of calcium sulfate, 10-17 parts of calcium chloride, 20-35 parts of vegetable oil, 1-10 parts of titanium dioxide, 1-5 parts of glutathione, and 1-3 parts of dispersant. This invention solves the problems of slow effectiveness, short duration of action, and low plant safety in current macadamia nut leaf moss control methods, achieving highly efficient control.

The application belongs to the technical field of biological medicine and medical cosmetology, and particularly relates to a kind of composition for mitochondrial protection type cosmetology and skin repair based on hydrogel and application thereof, the composition comprises a mitochondrial protection system and a synergistic active ingredient, the mitochondrial protection system comprises a hydrogel and a mitochondrial protection solution, the hydrogel comprises a synthetic polymer, and the mitochondrial protection solution comprises 200-300 mM mannitol, 0.2-5 mM ATP and 0-5% w / v trehalose; the synergistic active ingredient comprises a mixture of one or more of 0.01-0.1% ascorbyl palmitate, 0.1-1 mM glutathione and 0.5-2% nicotinamide. The composition is applied to microneedle repair, ice needle patch preparation and mask preparation and other aspects; through microneedle repair, ice needle patch or mask and other forms, effective delivery of mitochondria is realized, all-round care and repair are provided for the skin, and the delivery capacity is improved.

The application relates to the technical field of biological medicine, and discloses a targeted ferroptosis-inducing conjugate as well as a preparation method and application thereof. The structural formula of the conjugate is as follows: the conjugate contains a prostate cancer targeting peptide segment WHDGFK, and is connected with an iron death-inducing antibacterial peptide KRIVKWIIKLLR through a disulfide bond, so that the conjugate has tumor targeting and microenvironment response release functions, not only endows the antibacterial peptide KRIVKWIIKLLR with tumor selective delivery capacity, but also realizes specific release of the active peptide in target cells by reducing the disulfide bond by using high-concentration glutathione (GSH) in tumor cells, thereby synergistically enhancing the antitumor effect and reducing the systemic toxicity.

The application discloses a method for detecting glutathione by using manganese-based chiral nanomaterials, and belongs to the field of chemical engineering, specifically to the field of analytical chemistry technology. The method comprises the following steps: taking a dispersion liquid of manganese-based chiral nanomaterials as a detection reagent, mixing the detection reagent with a sample containing glutathione to be detected, and comparing a circular dichroism spectrum signal with a standard curve to obtain the concentration of the sample containing glutathione to be detected; wherein a chiral ligand of the manganese-based chiral nanomaterials is ribose. Based on the circular dichroism spectrum detection, the detection wavelength band used is in the visible light region, is not easy to be disturbed, is sensitive, and the resolution can reach 3 muM, and the detection limit is 1.5 muM, which has important significance and value for promoting the development of the fields of optics, life science, medicine and the like.

This invention belongs to the field of biomedical materials and nanotechnology, specifically relating to the synthesis and application of a multifunctional mesoporous nanomaterial. The inventors synthesized a core-shell structured mesoporous nanoparticle using mesoporous silica nanoparticles and dopamine via emulsion epitaxy. The core of the core-shell structured mesoporous nanoparticle is S-nitrosoglutathione supported on mesoporous silica, and the outer shell is pentafluorophenyl chlorophyll supported on mesoporous polydopamine, forming MSN / G@mPDA / F nanoparticles. These nanoparticles rapidly increase in temperature to 45°C under 808 nm laser irradiation. o At temperatures above a certain temperature, S-nitrosoglutathione decomposes to produce nitric oxide. Further irradiation at 750 nm generates a large amount of reactive oxygen species, killing bacteria. This combination of photothermal and photodynamic therapy effectively inhibits bacterial growth. Therefore, this method exhibits excellent antibacterial effects and can play a role in the future treatment of bacterial infections.

The application provides a kind of mitochondrial function optimization additive for cell culture, preparation method and application, belong to cell culture technical field.The additive includes core active ingredient, auxiliary component and carrier, and the core active ingredient includes D-Arg-Cha-Lys-Phe-NH2 Polypeptide, ubiquinone-10 phosphate, reduced glutathione and alpha-lipoic acid.The application forms a multi-target core active system through the synergy of the four, realizes the triple synergistic protection of stabilizing mitochondrial cardiolipin, maintaining mitochondrial morphology and membrane potential, and reducing mitochondrial ROS, which can significantly improve cell culture quality.When applied, the mitochondrial cardiolipin content can be increased by more than 50%, the mitochondrial abnormality ratio can be reduced to less than 12%, the membrane potential positive cell ratio can be increased to more than 86%, the mitochondrial ROS level can be reduced by more than 40%, and the cell proliferation rate can be increased by more than 25%, which is suitable for in vitro culture of various eukaryotic cells, and the preparation process is simple and has no cytotoxicity.

This invention relates to the field of biomedical technology and discloses an amphiphilic nicotinamide adenine dinucleotide (NAD) + Couplings, nanomicelles and their applications. Amphiphilic NAD+ + Couplings have the following general structural formula: ; where NAD + R is a hydrophilic group, R is a hydrophobic antioxidant group, and phenylboronic acid derivative is the linking bond; R is selected from free radical scavengers, small molecule organoselenic compounds that mimic the function of glutathione peroxidase 4, iron chelating agents, 7-dehydrocholesterol, royal jelly acid, α-lipoic acid, oleic acid, or derivatives of the above molecules. In this invention, NAD... + Conjugates and their self-assembled nanomicelles possess functions such as promoting DNA repair, maintaining cellular homeostasis, and delaying aging. Simultaneously, nanomicelles can inhibit inflammation and oxidative stress, and can physically encapsulate hydrophobic active drugs, showing broad application prospects in inflammatory diseases, age-related diseases, organ damage, and the prevention and treatment of toxic side effects from chemotherapy drugs.

This invention discloses a GSH-functionalized carbon dots / rhodamine B ratiometric fluorescence system, its construction method, and its application in nitrite detection. GSH-CDs are synthesized via a one-step hydrothermal method by mixing citric acid, o-phenylenediamine, glutathione, and hydrochloric acid in water. Unreacted precursors and small molecules are removed by purification, and the GSH-CDs are obtained by freeze-drying. The ratiometric fluorescence system is constructed by mixing rhodamine B, GSH-CDs, and an acidic buffer solution. The fluorescence intensity ratio F of GSH-CDs to rhodamine B is established. 460 / F 590 With nitrite (NO2) ‑ A calibration curve between concentrations was obtained, and the NO2 in the actual sample was experimentally detected using the spiked recovery method. ‑ Content. Under acidic conditions, with NO2... ‑ With increasing concentration, the GSH-CDs / RhB fluorescence intensity ratio (I 460 / I 590 Significant changes occurred, with the fluorescence intensity ratio compared to NO2. ‑ There is a good linear relationship between concentrations. Compared with the detection platform based on GSH-CDs, the ratio fluorescence system provided by this invention has a lower detection limit and significantly higher sensitivity.

This invention discloses the application of the blueberry VcERF70 gene in improving cadmium tolerance in plants, belonging to the field of plant genetic engineering technology. The amino acid sequence of the VcERF70 protein is shown in SEQ ID NO.2, and the nucleotide sequence of its encoding gene is shown in SEQ ID NO.1. This invention constructs a plant overexpression vector containing the VcERF70 gene and genetically transforms it into blueberries. The resulting transgenic plants exhibit significantly reduced malondialdehyde and hydrogen peroxide content under cadmium stress, and significantly increased superoxide dismutase, catalase, peroxidase activities, and glutathione content. Experiments demonstrate that overexpression of the VcERF70 gene can effectively enhance the antioxidant defense capacity of blueberries, reduce cadmium stress-induced oxidative damage, and thus improve the blueberry's tolerance to cadmium. This invention provides a new gene resource and technical pathway for blueberry stress-resistant breeding.

The application relates to a composition for treating reflux esophagitis and application thereof. The composition comprises the following components in parts by weight: 2-8 parts of scutellaria baicalensis, 3-7 parts of licorice, 5-10 parts of beeswax and 80-120 parts of vegetable oil. The composition for treating reflux esophagitis contains scutellaria baicalensis, licorice, beeswax and vegetable oil, and through mutual cooperation among the components, the drug prepared from the composition can significantly increase the plasma motilin level of a patient, further increase the pressure of the lower esophageal sphincter, promote gastric emptying and prevent acid reflux, promote the recovery of reflux esophagitis, the esophagus of the patient is obviously improved, and no adverse reaction is found; meanwhile, the content of lipid peroxide can be obviously reduced, the activities of superoxide dismutase and glutathione peroxidase are increased, the esophageal mucosa is protected and treated, the pathological changes of the esophagus are obviously improved, and the treatment effect is excellent.

The application discloses a kind of synergistic antitumor porphyrin-based covalent organic framework heterojunction materials and preparation method thereof, belong to biological medicine technical field. 5,10,15,20-tetra (4-aminobenzene) -21H,23H-porphyrin and 2,5-divinyl benzene formaldehyde are added to organic solvent to carry out solvothermal reaction, and porphyrin-based covalent organic framework (pCOF) is obtained;pCOF is mixed with 1,2-ethanedithiol and heated to obtain thiolated modified pCOF;Thiolated modified pCOF is stirred with glutathione aqueous solution ultrasonically, then tetra-chloroauric acid is added to carry out reaction, and synergistic antitumor porphyrin-based covalent organic framework confined gold nanocluster heterojunction material, recorded as Au@pCOF, is obtained.The Au@pCOF prepared in the application can be used as a kind of biocompatible antitumor therapeutic agent, under the irradiation of 808 nm near-infrared light, active oxygen is efficiently generated and heat is released, photothermal and photodynamic synergistic enhancement is realized, tumor cells are significantly killed, and is used for space-time integration synergistic antitumor therapy.

This application belongs to the field of microbial technology, and mainly relates to a strain of *Pediococcus lactis* BPA03 and its applications. *Pediococcus lactis* BPA03 is classified and named *Pediococcus lactis*. Pediococcus acidilactici This *Pediococcus lactis* strain BPA03 was deposited on September 28, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 36109. It inhibits skin melanin deposition and improves UV photoaging, suppresses the gene expression levels of immune factors such as IL-1β, IL-6, IL-8, and TNF-α, improves the activities of superoxide dismutase, catalase, and glutathione peroxidase, inhibits vinorelbine-induced neutropenia, and improves the inhibition of immune factor expression induced by vinorelbine, thus exhibiting comprehensive anti-aging effects.

This invention relates to the field of polypeptide derivative technology, specifically a method for preparing S-acetylglutathione in an aqueous system. The method involves dissolving 10% glutathione in water, adding 1-acetylimidazole, and reacting in controlled portions of 20-30 times, with 2-minute intervals, until the glutathione residue reaches 0.02%. The pH is adjusted to 3 with hydrochloric acid, and ethanol is added stepwise with seed crystals for crystallization. The mixture is then cooled to 4°C and stirred for 2 hours. After filtration, the product is washed with 60% ethanol to obtain S-acetylglutathione. This method does not use organic solvents, exhibits high selectivity, extremely low substrate residue, high crystallization efficiency, a product purity of 98.8%, and a yield of 90.9%. The process is simple, environmentally friendly, and suitable for industrial production.

This invention relates to the field of nucleic acid release, specifically disclosing a rapid nucleic acid release reagent, kit, method, and application for animal tissue genotyping. The release reagent comprises a weakly alkaline reaction buffer, an enzyme-catalyzed reaction enhancer, a nonionic surfactant, a nucleic acid amplification enhancer, a first component composed of histidine, trehalose, and glutathione in a specific ratio, and a second component with a visual indicator function; a matching thermosensitive proteinase K formation kit is also included. The kit can be pre-packaged in multi-well reaction plates for improved ease of use. The release method of this invention achieves rapid release of nucleic acids from animal tissues through pre-incubation activation, mild-temperature lysis, and high-temperature enzyme inactivation steps. Simultaneously, the first component exhibits a strong synergistic effect with the thermosensitive proteinase K, effectively protecting the integrity of the nucleic acids.

The application provides a culture medium for producing dendrobium stem cell exovesicles and an application thereof, relates to the technical field of biology, and the culture medium for producing dendrobium stem cell exovesicles comprises 0.1-0.5 mg / L naphthalene acetic acid, 0.05-0.2 mg / L thidiazuron, 0.5-5 muM sodium selenate and 1-10 mg / L glutathione. The culture medium for producing dendrobium stem cell exovesicles and the method for preparing dendrobium stem cell exovesicles by using the culture medium for producing dendrobium stem cell exovesicles provided by the application have made significant progress in the yield and quality of dendrobium stem cell exovesicles. Meanwhile, the prepared dendrobium stem cell exovesicles have significant DPPH free radical scavenging capacity and improve the healing capacity of skin fibroblasts.

This invention discloses a culture medium and cultivation method for promoting the development of Haemonchus contortus larvae, belonging to the field of biotechnology. This invention discovers that compound vitamins and / or bovine liver extract powder as auxiliary components can promote the development of early fourth-stage larvae of Haemonchus contortus into late fourth-stage larvae and adults. This invention provides a culture medium and cultivation method for promoting the development of Haemonchus contortus larvae. The culture medium includes a basal culture medium and auxiliary components, including compound vitamins, bovine liver extract powder, pyruvate, non-essential amino acids, L-glutamine, fetal bovine serum, defibrinated sheep blood, and L-glutathione. The cultivation method includes the step of adding early fourth-stage larvae to the culture medium for cultivation. This invention enables the in vitro culture of adult Haemonchus contortus, which is beneficial for promoting the development of related drugs and vaccines for this parasite and has significant implications for the prevention and control of parasitic diseases.

This invention relates to a liposomal formulation based on myeloid cell regulation and chemotherapy enhancement, along with its preparation method and antitumor applications, belonging to the technical field of pharmaceutical formulations. The liposomal formulation uses liposomes as carriers to co-load the CD11b agonist leukadherin-1 (LA1) prodrug (DSPE-PEG2000-Azo-LA1) and the gemcitabine prodrug Gem-SS-Chol. DSPE-PEG2000-Azo-LA1 contains a hypoxia-responsive azobenzene linker, and Gem-SS-Chol contains a glutathione-responsive disulfide bond, enabling precise drug release within the tumor microenvironment. This formulation exhibits uniform particle size and good stability, synergistically exerting chemotherapeutic and immunomodulatory effects. It effectively inhibits the proliferation and migration of pancreatic cancer tumor cells, enhances tumor cell immunogenic cell death, regulates the phenotype of myeloid immune cells in the tumor immunosuppressive microenvironment, and improves the therapeutic effect of pancreatic cancer. This invention provides a novel and highly efficient liposomal formulation for pancreatic cancer treatment, focusing on myeloid cell regulation and chemotherapy enhancement.

The application discloses a kind of lysosome-targeting protein modulators based on upconversion nanomaterial-DNA nanostructure and preparation method thereof.The lysosome-targeting protein modulators are combined by covalent bond from carboxyl functionalized upconversion nanoparticles and amino-modified DNA nanostructure, mainly enter cancer cells through folate receptor, after near infrared light illumination and glutathione response, the target protein combined is transported into lysosome and degraded.The lysosome-targeting protein modulators of the application have the advantages of light affinity, high controllability and storage stability, are easy to be metabolized by organism, and have good application prospect in the field of cell biology and cancer targeted therapy.

This invention relates to a highly penetrating soluble microneedle patch containing small molecule PDRN. It comprises a functional needle body and a base. The functional needle body is prepared from components comprising the following parts by weight: 4-7 parts sodium hyaluronate, 1-3 parts PDRN, 1-2 parts p-hydroxyacetophenone, 1-3 parts hydroxypropyl cellulose, 2-4 parts glutathione, 0.1-0.3 parts tranexamic acid, and 5-7 parts centella asiatica. The base is prepared from a polymer matrix material. This invention is the first to add PDRN to a soluble microneedle patch, using PDRN of different molecular weights and concentrations to prepare soluble microneedle patches with different penetration properties. This effectively solves the problems of insufficient hardness, poor penetration, and poor penetration rate of existing soluble microneedle patches, enabling it to penetrate skin with different stratum corneum thicknesses and making it applicable to various skin conditions with a wide range of applications.

The application discloses an electroporation transfection protection fluid suitable for abalone embryo cell gene editing and application thereof, and belongs to the technical field of gene editing. The electroporation transfection protection fluid comprises a basic fluid and a cell protection agent; the basic fluid comprises NaCl, MgSO4, CaCl2, KCl, NaHCO3, NaBr, K2HPO4, glucose and glutathione; and the cell protection agent comprises polyethylene glycol 400. The electroporation transfection protection fluid can significantly reduce the deformity rate and mortality rate of abalone cells after electroporation technology, improve the gene transfection and gene editing efficiency, and reduce the cost of gene editing experiments, thereby laying a foundation for accelerating marine biological research and promoting environmental protection, species conservation and aquaculture.

This invention discloses a method for simultaneously determining glutathione and cysteine, relating to the field of health food and the detection of functional components in food. The method includes: serially diluting standard solutions of glutathione and cysteine, performing liquid chromatography analysis, and establishing a standard curve for concentration and peak area; preparing a mixed standard solution containing glutathione and cysteine, performing liquid chromatography analysis to establish a correspondence between target components and retention times; performing liquid chromatography analysis on the sample solution, obtaining the peak area of ​​each target component in the sample solution based on the correspondence, and calculating the content based on the peak area and the standard curve. This invention utilizes liquid chromatography technology, enabling the rapid and simultaneous determination of glutathione and cysteine ​​content in a single injection analysis. It is simple to operate, highly efficient, shortens the experimental cycle, reduces experimental costs, and is widely applicable to formulations such as gummies, tablets, and capsules.

This invention relates to the field of chitin extraction technology and discloses a method for extracting chitin from glutathione fermentation residue. The method includes: sequentially pressing, dehydrating, pulverizing, and vacuum drying the glutathione fermentation residue; adding alkali solution and reacting at a constant temperature to remove proteins, followed by washing to obtain deproteinized residue; adding concentrated sodium hydroxide solution and heating to carry out a deacetylation reaction, with concentrated sodium hydroxide solution added periodically during the reaction, followed by washing to obtain the deacetylated product; sequentially adding a decolorizing agent for decolorization and dilute hydrochloric acid solution to remove impurities, followed by washing with water, vacuum drying, and pulverizing to obtain the finished chitin product. By employing gradient alkali treatment and a constant-temperature alkali replenishment process, the degree of deacetylation of chitin is improved and main chain degradation is reduced; the multi-stage purification steps combining physical and chemical methods improve the purity of the finished product; simultaneously, protein byproducts are recovered and waste liquid is recycled, improving resource conversion rate and reducing waste liquid discharge.

This invention discloses a butyrate prodrug co-assembled with artesunate, its preparation method, and its application in kidney diseases, belonging to the field of biomedical technology. The invention first uses docosahexaenoic acid (DHA) as a hydrophobic framework, coupling it with 2,2'-dithiodiethanol to introduce disulfide bonds, and then grafts butyrate via esterification to obtain a butyrate prodrug. The butyrate prodrug and artesunate self-assemble through non-covalent interactions to obtain a co-assembled drug in nanoparticle form. This nanomedicine possesses excellent long-term in vivo circulation and passive targeted accumulation in the kidneys, and can achieve targeted drug release in response to the high-glutathione microenvironment within lesion cells. The two active ingredients synergistically exert metabolic regulation, anti-inflammatory, and antioxidant effects, inhibiting kidney inflammation and oxidative stress at multiple targets, protecting renal tubular epithelial cells, and effectively intervening in the pathological process of acute kidney injury. Furthermore, this drug has high biosafety, is simple to prepare, and is easy to scale up, showing broad application prospects in the prevention and treatment of kidney diseases.