766 results about "Singlet oxygen" patented technology

Method and device for treatment of skin conditions A method and apparatus for improving the cosmetic appearance of a region of skin 11 affected by Acne Vulgaris, Rosacea or similar skin condition by means of directing light radiation 12 from an illuminating device 1 on to the skin 11. The apparatus 10 comprises a control unit 9 that operates one or more LEDs 7 (light emitting diodes) of the illuminating device 1. Each dose of light radiation 12 lasts for at least 100 ms, during which time the skin 11 receives light energy from the LED(s) 7, which causes a photochemical reaction that stimulates the production of free radicals (singlet oxygen) that react with, and at least partially disable or destroy, bacteria that contribute to the symptoms of the skin condition. The light energy directed on to the skin 11 during any given period of 10 μs is less than 0.5 Jcm⁻², and during any given period of 100 ms is less than 5 Jcm⁻². Substantially no beneficial photo-thermal reaction occurs within the skin 11. Light having wavelengths around 405 nm and/or 585 nm is used. The duration of a single dose may be much longer than 100 ms and can last up to 10 hours (for overnight treatment).

Equi-dosed time-fractionated pulsed UVA is employed to irradiate a class of riboflavin/collagen mixture in the presence of copious oxygen to cause rapid crosslinking causing gelation of the riboflavin/collagen mixture in situ and to effect adhesion to underlying structure specifically ocular tissue such as scleral and corneal tissue. Irradiation according to an embodiment of the invention results in depletion of dissolved oxygen at a rate inversely related to irradiance and more particularly depletion of dissolved oxygen occurs rapidly during the process of generation/cross-linking of reactive oxygen species (ROS), specifically singlet oxygen, such that the pulsed fractionation of UVA radiation exposure increases cross-linking efficiency by allowing the re-diffusion of oxygen during pauses in exposure.

A method of synthesizing a doped carbonaceous material includes mixing a carbon precursor material with at least one dopant to form a homogeneous/heterogeneous mixture; and subjecting the mixture to pyrolysis in an inert atmosphere to obtain the doped carbonaceous material. A method of purifying water includes providing an amount of the doped carbonaceous material in the water as a photocatalyst; and illuminating the water containing the doped carbonaceous material with visible light such that under visible light illumination, the doped carbonaceous material generates excitons (electron-hole pairs) and has high electron affinity, which react with oxygen and water adsorbed on its surface forming reactive oxygen species (ROS), such as hydroxyl radicals and superoxide radicals, singlet oxygen, hydrogen peroxide, that, in turn, decompose pollutants and micropollutants.

Systems and methods for generating reactive oxygen species formulations useful in various oxidation applications. Exemplary formulations include singlet oxygen or superoxide and can also contain hydroxyl radicals or hydroperoxy radicals, among others. Formulations can contain other reactive species, including other radicals. Exemplary formulations containing peracids are activated to generate singlet oxygen. Exemplary formulations include those containing a mixture of superoxide and hydrogen peroxide. Exemplary formulations include those in which one or more components of the formulation are generated electrochemically. Formulations of the invention containing reactive oxygen species can be further activated to generate reactive oxygen species using activation chosen from a Fenton or Fenton-like catalyst, ultrasound, ultraviolet radiation or thermal activation. Exemplary applications of the formulations of the invention among others include: cleaning in place applications, water treatment, soil decontamination and flushing of well casings and water distribution pipes.

The present invention provides methods and compositions for photodynamic therapy. The composition comprises ceramic nanoparticles in which a photosensitive drug/dye is entrapped. The ceramic nanoparticles are made by formation of a micellar composition of the dye. The ceramic material is added to the micellar composition and the ceramic nanoparticles are precipitated by alkaline hydrolysis. The precipitated nanoparticles in which the photosensitive dye/drug is entrapped can be isolated by dialysis. The resulting drug doped nanoparticles are spherical, highly monodispersed, and stable in aqueous system. Irradiation with light of suitable wavelength of the photosensitizing drug entrapped inside nanoparticles resulted in generation of singlet oxygen, which was able to diffuse out through the pores of the ceramic matrix. The drug loaded ceramic nanoparticles of the present invention can be used as drug carriers for photodynamic therapy.

The COIL gain generator system includes a reactor for producing singlet delta oxygen and a mechanism for mixing high momentum diluent with the singlet delta oxygen and with iodine for producing a high momentum, low static temperature mixture of the singlet delta oxygen, diluent and iodine. The singlet delta oxygen and the iodine react to produce excited iodine atoms which can lase efficiently due to the low static temperature and can, after lasing, recover to high pressure in a diffuser due to the high momentum of the mixture. This provides the capability of using a chemical pump which allows a completely sealed system with no outside exhaust.

Light is administered during photodynamic therapy (PDT) for an extended period of time at a plurality of sites distributed within the abnormal tissue of a tumor. A clinical study has shown that a substantially greater volume of abnormal tissue in a tumor is destroyed by the extended administration of light therapy from a plurality of probes than would have been expected based upon the teaching of the prior art. In this process, a plurality of light emitting optical fibers or probes are deployed in a spaced-apart array. After a photoreactive agent is absorbed by the abnormal tissue, the light therapy is administered for at least three hours. The greater volume of necrosis in the tumor is achieved due to one or more concomitant effects, including: the inflammation of damaged abnormal tissue and resultant immunological response of the patient's body; the diffusion and circulation of activated photoreactive agent outside the expected fluence zone, which is believed to destroy the abnormal tissue; a retrograde thrombosis or vascular occlusion outside of the expected fluence zone; and, the collapse of the vascular system that provides oxygenated blood to portions of the tumor outside the expected fluence zone. In addition, is possible that molecular oxygen diffusing and circulating into the expected fluence zone is converted to singlet oxygen during the extended light therapy, causing a gradient of hypoxia and anoxia that destroys the abnormal tissue outside the expected fluence zone.

The present invention provides for a method to increase the triplet yield of a photosensitizer by the coupling to metal surface plasmons which leads to increased singlet oxygen generation by electric field enhancement or enhanced energy absorption of the photosensitizer. The extent of singlet oxygen enhancement can be tuned for applications in singlet oxygen based clinical therapy by modifying plasmon coupling parameters, such as metallic nanoparticle size and shape, photosensitizer/metallic nanoparticle distance, and the excitation wavelength of the coupling photosensitizer.

The present invention provides fluorogenic probes and corresponding fluorescent compounds, methods of using the probes, compounds and kits that include the probes.

Antimicrobial compositions containing a singlet oxygen-generating agent and a lubricant exhibit very effective antimicrobial activity against a variety of organisms, including Gram-positive bacteria, Gram-negative bacteria, fungi and yeast. The compositions have particular utility as beverage container lubricants. The passage of a container along a conveyor is antimicrobially lubricated by applying a lubricant to at least a portion of the container-contacting surface of the conveyor or to at least a portion of the conveyor-contacting surface of the container and generating singlet oxygen in situ. This method provides effective control of microbes on a beverage conveyor line, at rates comparable to purely chemical biocidal systems.

A series of organometallic complexes and the singlet oxygen sensitization properties of these complexes are provided. Complexes with acetylacetonate ligands give singlet oxygen quantum yields near unity, whether exciting the ligand-based state or the lowest energy excited state (MLCT+³LC). The singlet oxygen quenching rates for these β-diketonate complexes are small, roughly three orders of magnitude slower than the corresponding phosphorescence quenching rate. Similar complexes were prepared with glycine or pyridine tethered to the Ir(III) center (i.e. (bsn)₂Ir(gly) and (bt)₂Ir(py)Cl, where gly=glycine, and py=pyridine). The glycine and pyridine derivatives give high singlet oxygen yields.

The present invention provides organically modified silica (ORMOSIL) nanoparticles into which have been incorporated two-photon absorption dye molecules. The two photon absorption dye displays a unique aggregation induced fluorescence enhancement behavior. As a result ORMOSIL nanoparticles with high amounts of the dye can be prepared. These particles can be used for imaging. In one embodiment, the nanoparticles can additionally have incorporated therein a photosensitizer. The photosensitzer can be activated by intraparticle fluorescence resonance energy transfer (FRET) from the dye aggregates resulting in enhanced fluorescence and singlet oxygen generation from photosensitizer under two-photon excitation conditions. Such nanoparticles can be used for photodynamic therapy applications.

The invention relates to a singlet state oxygen generation method, and belongs to the technical field of chemical oxygen-iodine lasers. The method comprises the following steps: mixing an alkaline ionic liquid insoluble in water with an aqueous hydrogen peroxide solution to form a two phase transfer system, and reacting the transferred ionic liquid phase with chlorine to generate singlet state oxygen in order to realize water and singlet state oxygen isolation. The method is helpful for reducing the quenching effect of water on singlet state oxygen, and reduces the content of water in the singlet state oxygen, and the alkaline ionic liquid has an enrichment effect on hydrogen peroxide to make effective solving of the bottleneck problem of hydrogen peroxide concentration restriction in the raw material regeneration process of chemical oxygen-iodine lasers under traditional technological conditions be possible, so the method is of great significance to improve the singlet state oxygen yield of the chemical oxygen-iodine lasers, reduce the water content of the singlet state oxygen and solve the raw material regeneration problem.

The present invention relates to a liposome combination, which wrapped hydrophilic drugs in water layer and wrapped hydrophobic drugs in lipid bilayer; hydrophobic drugs are photosensitizers. Using light with appropriate wavelength to activate the photosensitizer in the hydrophobic layer can result in the production of singlet oxygen and the free radical, and cause the oxidizing and breaking of the carbon chain of the phospholipid, and influences the stability of the liposome and the releases of the drug. The singlet oxygen and the free radical will attack the cancer cells at the same time as a result of combining the photodynamic- and chemo-effects.

The invention relates to a homogeneous immunoassay kit, detection method and application thereof in the technical field of biology. The kit comprises a reagent I, a reagent II, a reagent III and a reagent IV, wherein the reagent I contains a first counterpart, and the first counterpart is a known antigen or a known antibody specially combined with an antigen to be detected in a sample to be detected; the reagent II contains a receptor which can react with singlet oxygen to generate a detectable signal and a second counterpart combined with the receptor; the reagent III contains a third counterpart specially combined with the first counterpart; the reagent IV contains a donor which can generate the singlet oxygen in an excitation state; the surface of the third counterpart is coated with biotin, and the surface of the donor is coated with streptavidin. By utilizing combined detection of the kit and the homogeneous immunoassay method of the antigen and the antibody, the screening difficulty of raw materials is reduced, and the detection sensitivity is promoted at the same time.

Methods are disclosed for determining minute quantities of an analyte in a medium suspected of containing the analyte. One method comprises treating a medium suspected of containing an analyte under conditions such that the analyte, if present, causes a substrate having an oxidant cleavable linker and a photosensitizer to come into close proximity. The photosensitizer generates singlet oxygen which oxidatively cleaves the linker to form a product which can be detected in a sandwich detection assay such as LOCI. The amount of product detected is related to the amount of analyte in the medium. Compositions, kits, and compounds are also disclosed.

Disclosed is a fluorescent conjugate comprising a biological reagent, a fluorescent molecule, and a means for impeding phototoxic degradation of the biological reagent. The impeding means can be a cross-linking substance having a long molecular distance, whereby the cross-linking substance links the fluorescent molecule and the biological reagent; a quencher of singlet oxygen; a quencher of a free radical; or a combination thereof. Also disclosed is a solution comprising a fluorescent conjugate of a biological reagent and a fluorescent molecule together with an oxygen depleting system.

The present invention relates to a new-type singlet oxygen europium fluorescent probe and its application. It is a coordination compound formed by using trivalent europium ion Eu3+ and 2, 2í»:6í»2íŒ-ditripyridine skeleton structure ligand containing functional group. Said invention also provides the structure formula of the described ligand. Said coordination compound can be used for making fluorescent determination of singlet oxygen.

The invention discloses a two-photon fluorescent probe capable of targeting mitochondria and a preparation method and application of the probe. The structure of the two-photon fluorescent probe is shown as a formula I, wherein R represents a conjugated structure, R' represents alkyl or aryl, R'' represents hydrogen, methyl or methoxy, X represents halogen anions or bi-trifluoromethane sulfonimideanions or hexafluoro-phosphoric acid anions or tetra-fluoroboric acid anions or trifluoromethanesulfonic acid anions. The compound has stronger two-photon excitation fluorescence, a good aggregation-induced luminescence effect, a great mitochondrial targeting function and excellent photoinduced singlet oxygen generation capability.

The present invention relates to new europium complex fluorescent probe for the imaging determination of singlet oxygen in live cell and its application. The fluorescent probe is complex formed with trivalent europium ion Eu3+ and organic ligand [4'-(10-methyl-9-anthryl)-2, 2':6'2''-tripyridyl-6, 6''-dimethyl amino] tetracetic acid as shown. The complex can enter to live cell simply in the presence of photosensitizer and capture singlet oxygen in cell specifically, leading to obviously enhanced fluorescence strength of the complex for the fluorescent determination of singlet oxygen in live cell.