187 results about "Mitochondrial targeting" patented technology

The invention relates to the technical field of pharmaceutical preparations, and discloses a composition with liver protection and antioxidation effects and a preparation method thereof.The composition is prepared from galactosamine modified astragalus membranaceus exosome, schisandra chinensis nanocrystals, liquorice-chitosan nanoparticles, lactobacillus acidophilus freeze-dried powder, beta-(1, 3) / (1, 3, 4-tetramethyl-1, 3-pentanediol monoisobutyrate) sourced from saccharomyces cerevisiae, and beta-(1, 3, 4-tetramethyl-1, 3-pentanediol monoisobutyrate). And (6) a dextran, grape exosome-curcumin hybrid vesicle freeze-dried powder, a curcumin-mitochondrial targeting peptide compound, a resveratrol-hydroxypropyl-beta-cyclodextrin inclusion compound and reduced panthenol. According to the liver-protecting and anti-oxidation composition disclosed by the invention, multi-dimensional intervention on liver protection is realized through an elaborately designed ternary system. The system is composed of a traditional Chinese medicine extract, a microorganism-plant exosome combination and a high-bioavailability antioxidant, all the parts have a synergistic effect, and the liver protection effect is remarkably improved.

The invention belongs to the technical field of biological medicine, and discloses an engineering bionic exosome with an anti-aging effect and a preparation method and application thereof, and the preparation method comprises the following steps: (S1) preparing a bionic cell membrane modified targeting ligand: adding collagen, mitochondrial targeting polypeptide, a condensing agent and 4-dimethylaminopyridine into an organic solvent, and stirring to obtain the bionic cell membrane modified targeting ligand; activating under a protective atmosphere, and then adding lysine into the system to react, so as to obtain a bionic cell membrane modified targeting ligand; and (S2) embedding the bionic cell membrane modified targeting ligand into the engineered exosome bionic membrane, and entrapping an active substance to form the engineered bionic exosome. The exosome provided by the invention has better skin permeability, mitochondrial targeting effect and cell endocytosis effect, has better protection effect on mitochondria, and can realize efficient delivery of active substances.

The invention discloses a beta-lactoglobulin fluorescent probe as well as a preparation method and application thereof, the structural formula of the probe is (C30H25N4I), the probe is combined with beta-lactoglobulin through non-covalent interaction, a fluorescence signal is triggered to be remarkably enhanced, and low-background fluorescence detection of the beta-lactoglobulin is realized. The probe has low cytotoxicity and excellent mitochondrial targeting ability, and provides a powerful tool for visualization of beta-lactoglobulin in living cells. After being combined with beta-lactoglobulin, a sample can generate obvious color change under the irradiation of an ultraviolet lamp, and the change can be used for evaluating the content of active beta-lactoglobulin in the sample, so that the rapid detection of the milk protein level in a beverage is realized. The beta-lactoglobulin fluorescent probe disclosed by the invention has a wide application prospect in the fields of biological imaging, milk protein detection, beverage quality control and the like.

The invention discloses a group of mitochondrial signal peptides and application thereof, and a group of signal peptides with a mitochondrial targeting function is obtained for the first time by screening endogenous mitochondrial key functional proteins of rhodotorula. The invention further realizes expression of a target gene through mitochondrial signal peptide mediation, realizes expression of a target product synthetic pathway gene in rhodotorula mitochondria, and successfully constructs synthetic pathways of terpene such as limonene, linalool, patchouli alcohol and kaurene, branched chain alcohol such as isobutanol and organic acid such as 3-hydracrylic acid. Further, carbon metabolic flow of pyruvic acid flowing to the target product is dredged, and synthesis of the mitochondrial region indoor target product is finally realized. The characterization of a group of rhodotorula mitochondrial signal peptides disclosed by the invention can realize the synthesis of mitochondrial regional chamber target products, and lays a foundation for the research on the realization of metabolic engineering transformation in subcellular organelles.

The invention provides an ovary-targeting ROS response type SS31 nano-particle and a preparation method and application thereof, and belongs to the field of biomedicine.The ovary-targeting ROS response type SS31 nano-particle is constructed by utilizing a porous metal organic framework to carry hyaluronic acid and utilizing an ROS response type thioketone linker to be combined with mitochondrial targeting peptide SS31, and the ovary-targeting ROS response type SS31 nano-particle is used for preparing the ovary-targeting ROS response type SS31 nano-particle. And the SS31 is targeted and delivered to the ovarian tissue. The nanoparticles target ovary and slowly release SS31 under the action of ROS, so that oxidative stress of oocytes and granular cells caused by female reproductive system diseases such as premature ovarian failure, polycystic ovarian syndrome and the like is reduced, and the mitochondrial function of the oocytes and the granular cells is improved; and a foundation is laid for the subsequent clinical treatment of diseases related to the mitochondrial dysfunction of the female reproductive system by using the SS31.

The invention discloses a dual-targeting macrophage membrane nano system and a preparation method and application thereof, and belongs to the field of biological medicine, MKA is wrapped in a macrophage membrane after AuNPs and mitochondrial targeting peptide KLA are connected. The preparation method comprises the following steps: firstly synthesizing AuNPs and modifying, and then wrapping with a macrophage membrane. In treatment, the compound can realize double targeting of tumor cells and mitochondria, enhance radiation-induced DNA damage, inhibit DDR, activate immune pathways and promote T cell infiltration, and can also consume glutathione and amplify oxidative stress to enhance immune effect, and the dose enhancement ratio of the compound in cooperation with 8GyX-rays reaches 3.1. In addition, the MKA is good in biocompatibility in vivo, long in blood circulation time, high in tumor targeting and capable of being quickly cleared through the kidney. The invention provides a new way for tumor radioimmunotherapy, and is helpful for promoting the progress of clinical tumor treatment.

The invention provides a traceable mitochondrial targeting nano system as well as a preparation method and application thereof, and belongs to the field of medicines. The traceable mitochondrial targeting nano system constructed by the invention can continuously release resveratrol and maintain the photochemical stability of resveratrol. The nano system shows efficient cellular uptake and accurate mitochondrial targeting ability, and promotes removal of mitochondrial ROS and adjustment of mitochondrial autophagy, so that vascular leakage and angiogenesis are relieved, in-vivo microvascular hyperplasia and retina degeneration are remarkably inhibited, and the curative effect is improved. The distribution of the compound in fundus can be monitored in real time through clinical ophthalmological diagnosis equipment, and the compound has a good application prospect in preparation of drugs for preventing and / or treating diabetic retinopathy.

The invention belongs to the technical field of drug system delivery, and particularly relates to a radiotherapy sensitization microneedle and application thereof. The invention relates to a design of a mitochondrial targeting photosensitive microneedle system and an application of the mitochondrial targeting photosensitive microneedle system in melanoma radiosensitization radiotherapy. According to the invention, an intelligent delivery platform of the microneedles (MNs) is fused with mitochondrial targeted photodynamic therapy, and a new normal form is developed for radiosensitization therapy of melanoma. According to the invention, a soluble hyaluronic acid (HA) microneedle array is adopted, and a mitochondrial targeting photosensitizer is loaded. After penetrating through the cuticle, the microneedle is rapidly dissolved in the corium layer, the photosensitizer is released, and mitochondrial targeted enrichment is achieved. Then, under the condition of 660 nm illumination, the photosensitizer generates active oxygen in a mitochondrial matrix to directly destroy the mitochondrial structure and function, so that the mitochondrial ROS storm is induced, and the tumor radiotherapy sensitivity is improved.

The invention provides a combined use of a hyaluronic acid-bilirubin conjugate and honokiol in preparation of a medicine for treating osteoarthritis. The invention provides a honokiol-loaded nanoparticle, which is characterized in that a hyaluronic acid-bilirubin conjugate and honokiol are used as raw materials to prepare a nanoparticle HKL-PNPs, and then the nanoparticle HKL-PNPs is modified by a mitochondrial targeting peptide to obtain the honokiol-loaded nanoparticle HKL-MPNPs. The invention also provides a bionic nanoparticle for treating bone joints. The bionic nanoparticle is prepared by wrapping the honokiol-loaded nanoparticle with an engineered cartilage cell membrane (ECM). According to the invention, cartilage cells with high expression of IL-1R2 are obtained through a gene editing method for the first time, and mitochondrial targeted nanoparticle cores loaded with Sirtuin-3 agonists are coated by cell membranes of the cells, so that the bionic nano preparation is obtained and is used for treating OA. The prepared bionic nano preparation not only can neutralize IL-1beta mediated inflammatory reaction, but also can recover Sirtuin-3 mediated mitochondrial function imbalance, so that the result of synergistic treatment of OA is achieved.

The invention relates to the technical field of biomedical materials, in particular to an alendronate-modified ZIF-8 encapsulated TPP-carbon dot bone targeting nano-material and a preparation method and application thereof.The alendronate-modified ZIF-8 serves as a carrier, TPP-modified carbon dots are encapsulated in the carrier, citric acid, urea and cerium salt serve as raw materials of the TPP-modified carbon dots, and the alendronate-modified ZIF-8 encapsulated TPP-carbon dot bone targeting nano-material is prepared through a one-step method. After cerium-doped carbon quantum dots are synthesized through a hydrothermal method, TPP is grafted through an amide method, alendronate endows the material with a bone targeting function, and TPP modified carbon dots endow the material with antioxidant and mitochondrial targeting functions. According to the invention, the bone targeting property is excellent, and bone focuses are efficiently enriched; the anti-oxidation and anti-inflammatory capacities are high, and the infected microenvironment can be improved; zIF-8 packaging is stable, and functional components can be controllably released; multiple components cooperate to promote bone repair, accumulation of non-target organs is little, and the composition is safe, reliable and suitable for treatment of various bone-related diseases.

The invention discloses a self-assembled PROTAC nano material based on natural product mitochondria targeting, which is a nano particle prepared from mitochondria targeting molecules, a photosensitizer and PROTAC as raw materials through a nano precipitation method. The mitochondrial targeting molecule is berberine, the photosensitizer is hypericin, and the PROTAC is dBET57; the ratio of the amount of substance of the dBET57 to the amount of substance of the berberine is (1-10): (1-10), and the ratio of the amount of substance of the dBET57 to the amount of substance of the hypericin is (1-10): (1-10). The nano material can be used for blocking multiple energy metabolism pathways of tumor cells, including glycolysis and oxidative phosphorylation, meanwhile, mitochondria is damaged, ferroptosis is induced, and the anti-tumor capacity of pharmacodynamic molecules is improved; bRD4 in tumor cells and downstream carcinogenic protein c-Myc of the BRD4 can be efficiently degraded. The invention also discloses an application of the nano material in preparation of antitumor drugs.

The invention discloses a layered programmable DNA nano device as well as a preparation method and application thereof. The layered programmable DNA nano device HPDN disclosed by the invention is specially used for space-time selective imaging of mitochondrial miRNA in tumor cells. The layered programmable DNA nano device HPDN is composed of a mitochondrial specific DNA tetrahedron and two cage-shaped HCR hairpin probes, and mitochondrial targeting is achieved through triphenylphosphine (TPP) modification. The HPDN realizes miRNA detection with high sensitivity and specificity by utilizing signal amplification of APE1 activation, ultraviolet light (UV) activation and miR-21 triggering in cells. The technology has the advantages of simplicity and convenience in operation, good biocompatibility, real-time dynamic monitoring and the like, and a new strategy is provided for early diagnosis and treatment monitoring of tumors.

The invention discloses an iron-based metal nano diagnosis and treatment agent as well as a preparation method and application thereof. The iron-based metal nano diagnosis and treatment agent comprises an iron-based metal nano material and a diagnosis and treatment agent loaded with therapeutic nuclide 177Lu and / or a micromolecular chemotherapeutic drug F16. The iron-based metal nano diagnosis and treatment agent contains an iron element, can be used for MRI imaging and can induce tumor ferroptosis at the same time, radionuclide 177Lu can emit gamma rays for SPECT imaging and can emit beta rays for tumor internal radiotherapy, tumor mitochondria targeting small molecule medicine F16 has the fluorescence characteristic and can be used for tumor real-time fluorescence imaging, and the application range is wide. Meanwhile, a mitochondrial targeting tumor killing effect is achieved. Therefore, the iron-based metal nano diagnosis and treatment agent disclosed by the invention can realize comprehensive collaborative treatment of intratumor radiotherapy, targeted chemotherapy and ferroptosis under the guidance of MRI (Magnetic Resonance Imaging), SPECT (Single Photon Emission Computed Tomography) and multi-mode imaging of fluorescence imaging, and improves the precise diagnosis and treatment of tumors.

The present invention discloses a mitochondrially targeted fluorinated ionizable lipid and its use as a mitochondrial gene delivery vector. A mitochondrially targeted fluorinated ionizable lipid is a compound of formula (I), or a pharmaceutically acceptable salt, stereoisomer, or tautomer thereof, wherein: a = an integer from 2 to 8, b = an integer from 4 to 8; and R1 is an ionizable lipid head group, comprising a mono- or polyamine containing a primary or tertiary amine structure. A mitochondrially targeted lipid carrier comprises the fluorinated ionizable lipid described herein, a cationic lipid, an auxiliary lipid, a structural lipid, and a functional polymer conjugated lipid modified with a mitochondrial targeting group. The fluorinated ionizable lipid gene delivery vector with mitochondrial targeting capability provided by the present invention has excellent biocompatibility, can simultaneously increase the cellular uptake and mitochondrial accumulation of gene drugs, improve the efficiency of mitochondrial gene transfection, and achieve efficient delivery of gene drugs to the mitochondrial matrix. #imgabs0#

The invention discloses a mitochondrial targeting and apoptosis-inducing photosensitive iridium complex and a preparation method thereof, and particularly discloses a 2, 2 '-bipyridine compound, a cyclometalated iridium complex using the compound as a ligand, a preparation method and application of the 2, 2'-bipyridine compound as an antitumor drug. The cyclometalated iridium complex provided by the invention takes a 2, 2 '-bipyridine compound as a ligand, can selectively induce tumor cell apoptosis under an illumination condition, can be used for preparing antitumor drugs, and has a great potential value in the field of photodynamic treatment of tumors.

The application discloses a green preparation method of a medium-chain acylated hydroxytyrosol lipoid-triphenylphosphine derivative and application of the derivative in mitochondrial-targeted antioxidation, and relates to the following steps: taking hydroxytyrosol and saturated halogenated fatty acids with medium-chain length C8-C12 as raw materials, and synthesizing a medium-chain hydroxytyrosol lipoid compound through a low-field ultrasonic coupling immobilized lipase synergistic directional alcohol hydroxyl acylation reaction; and further synthesizing the lipoid compound through high-speed stirring reaction with a triphenylphosphine compound to obtain a medium-chain acylated hydroxytyrosol lipoid-triphenylphosphine derivative. The derivative has the antioxidation effect of mitochondrial-targeted enrichment and active oxygen radical scavenging. The hydroxytyrosol derivative prepared by the application has the characteristics of green efficiency, directional bonding and simple operation. The acylation reaction of hydroxytyrosol does not need to involve flammable and explosive, easily oxidized and corrosive concentrated sulfuric acid and concentrated nitric acid, and the medium-chain acylated hydroxytyrosol lipoid-triphenylphosphine derivative has mitochondrial targeting, can be used as a potential mitochondrial nutritional supplement product, and has a wide application prospect in the health care functions of anti-aging, cardiovascular protection, diabetes prevention and the like.

The invention relates to the technical field of bone regeneration bioactive components, in particular to a monatomic nano-enzyme targeting mitochondria and accelerating bone regeneration and a preparation method thereof.The monatomic nano-enzyme comprises Fe / Cu monatomic double active sites, triphenylphosphine (TPP) molecules targeting the mitochondria and a porous nano-carrier, and the porous nano-carrier is a dendritic mesoporous silica nano-particle (DMSN). The invention effectively overcomes the defects of poor repair effect and long repair period caused by insufficient mitochondrial targeting, low catalytic efficiency and single function in the existing bone regeneration technology.

This invention relates to the field of bionanomaterials technology, and particularly to mitochondrial-targeting nanomaterials capable of releasing HNO, their preparation methods, and applications. The preparation method of the mitochondrial-targeting nanomaterials capable of releasing HNO includes the following steps: synthesis of a silanized HNO donor: hydroxylamine hydrochloride is dissolved in a mixed solvent of anhydrous tetrahydrofuran and anhydrous pyridine, and a dichloromethane solution of 2-(4-chlorosulfonylphenyl)ethyltrimethoxysilane is added. The mixture is stirred at room temperature, and after post-treatment, a silanized HNO donor is obtained; synthesis of aminated carbon dots: chitosan, ethylenediamine, and mercaptosuccinic acid are dispersed in deionized water, and concentrated hydrochloric acid is added and stirred until dissolved. This preparation method can prepare mitochondrial-targeting nanomaterials capable of releasing HNO, achieving precise delivery, controlled release, and visual tracking of HNO to cardiac mitochondria, solving the technical problems of traditional HNO donors' inability to achieve stable loading on carriers and lack of mitochondrial targeting ability.

The present invention provides a D-D-π-A type organic photosensitizer and a synthesis method and application thereof, belonging to the field of photosensitizer and photodynamic therapy application of tumors. The present invention uses triphenylamine and carbazole as electron donors, malononitrile as an electron acceptor, and thiophene as an electron π bridge to synthesize an AIE type photosensitizer with mitochondrial targeting function through a series of simple organic reactions. The AIE type photosensitizer can be used in the synergistic photodynamic and ferroptosis treatment of tumors. The photosensitizer can realize tumor cell imaging and target mitochondria, and can selectively exert photodynamic and ferroptosis effects on tumor cells. It has good therapeutic effect and biosafety, and has strong application prospects in non-invasive tumor treatment.

The invention discloses an organic compound biological probe and a preparation method and application thereof, wherein the organic compound biological probe is In-AIE, and the method comprises: dissolving rutin in acetonitrile, and then adding 4-formyl pyridine and n-tributylphosphine for reflux reaction to obtain compound 1; dissolving 1,1,2-trimethyl-1H-benzo[e]indole and iodoethane in anhydrous acetonitrile for reflux to obtain compound 2; adding 2,4-dihydroxybenzaldehyde and anhydrous potassium carbonate to anhydrous acetonitrile and then dropping 1,4-dibromobutane for reflux reaction to obtain colorless oily compound 3; dissolving compound 2 and compound 3 in anhydrous ethanol for reflux reaction to obtain solid compound 4; dissolving compound 1 and compound 4 in anhydrous acetonitrile for reflux reaction and then purifying to obtain solid In-AIE. It can quickly enter cells within 1 min, has strong anti-photobleaching ability, and integrates multi-channel imaging, pH detection, mitochondrial targeting and photodynamic therapy.

The present invention discloses a dual-targeted macrophage membrane nanosystem, its preparation method and application, which belongs to the field of biomedicine. MKA is formed by connecting AuNPs with the mitochondrial targeting peptide KLA and then encapsulating it in the macrophage membrane. During preparation, AuNPs are first synthesized and modified, and then encapsulated with the macrophage membrane. During treatment, it can dual-target tumor cells and mitochondria, enhance radiation-induced DNA damage, inhibit DDR, activate immune pathways, promote T cell infiltration, and consume glutathione and amplify oxidative stress to enhance immune effects. The dose enhancement ratio synergistic with 8GyX-rays is 3.1. In addition, MKA has good biocompatibility in the body, a long blood circulation time, high tumor targeting, and can be quickly cleared through the kidneys. This invention provides a new approach for tumor radioimmunotherapy and helps promote progress in clinical tumor treatment.

The present invention relates to base editing of mitochondrial DNA. More specifically, the present invention relates to: a method for editing mitochondrial DNA bases by using a mitochondrial targeting sequence (MTS); and a base editing system used in the method. The base editing system comprises a DNA binding protein and a deaminase, or polynucleotides encoding these, and does not include an affinity tag or a polynucleotide encoding same. The present invention is useful for editing mitochondrial DNA bases of animal cells or plant cells.

The invention discloses a platinum (IV)-internal peroxy prodrug molecule and application thereof, and belongs to the technical field of biological medicine. The series of molecules are characterized by being Pt (IV) complexes modified by internal peroxide and mitochondrial targeting groups. The synthesis method sequentially comprises the following steps: carrying out oxidation and hydroxyl modification on a platinum drug, introducing a mitochondrial targeting group and a singlet oxygen carrier through an esterification reaction, and carrying out a photosensitive reaction to prepare the endoperoxide. The series of molecules are reduced into Pt (II) drugs in a tumor microenvironment, singlet oxygen is released at the same time, a mitochondrial targeting group can accurately deliver the treatment molecules, and cancers are efficiently treated. A representative molecule Oxa-EM is selected for instance analysis, the molecule generates a Pt (II) complex with anticancer activity while releasing singlet oxygen, and the singlet oxygen-Pt combined anticancer purpose is achieved. Probes DPBF and SOSG are selected to prove that the molecule releases singlet oxygen, and high performance liquid chromatography is utilized to successfully prove that the Pt (II) complex is released in a reducing environment. Further cell and animal level experiments verify that the molecule has excellent anti-tumor efficacy and safety. The invention discloses design, synthesis and biological test of platinum (IV)-peroxy prodrug molecules, and the platinum (IV)-peroxy prodrug molecules have a wide anticancer application prospect.

The invention relates to the technical field of biology, in particular to a polypeptide compound, a medicine for preventing and treating eye diseases and application. According to the present invention, a mitochondrial targeting module (triphenylphosphine) and a superoxide-initiated persulfide donor (SOPD) are integrated into a specific polypeptide to obtain the ROS-responsive mitochondrial targeting polypeptide compound; experiments show that the polypeptide compound has remarkable oxidative stress resistance, can effectively relieve RGC damage caused by intraocular hypertension, has a good prevention and treatment effect on glaucoma optic neuropathy, has the prevention and treatment effect obviously improved compared with that of other polypeptide compounds with similar structures, and has good biocompatibility and biological safety.

The invention belongs to the field of biological nano medicines, and particularly relates to a conductive polymer nano enzyme as well as a preparation method and application thereof. According to the conducting polymer nano-enzyme, polyvinyl alcohol, polypyrrole and polythiophene serve as basic frameworks, different metal ions are coordinated, a conducting polymer nano-enzyme library is constructed, ruthenium-based polymer nano-enzyme Ruzyme is screened out through a series of enzyme activity characterization, the Ruzyme is subjected to mitochondrial targeting modification to obtain ICT-Ruzyme, and the ICT-Ruzyme is subjected to myocardial targeting peptide modification to obtain CICT-Ruzyme. The conductive polymer nano-enzyme prepared by the invention is uniform in size, not only has relatively good conductivity and stability, but also has excellent active oxygen and active nitrogen removal capability, and the conductive polymer nano-enzyme modified by mitochondrial targeting and myocardial targeting peptides can more effectively target myocardial cells, so that the treatment effect is improved. And Fe < 2 + > accumulation and lipid peroxidation which are related to myocardial cell ferroptosis induced by lipopolysaccharide are reduced, so that sepsis cardiomyopathy is effectively relieved.

This application discloses a preparation method and application of injectable hydrogel microspheres. By integrating the hydrogel drug delivery system with liposome microparticles obtained by microfluidic technology, NMN is encapsulated by liposome material, and the mitochondrial targeting peptide SS-31 is introduced into the liposome to achieve a more sustained and stable release, thereby enabling NMN to selectively target cell mitochondria and improve the application effect.

The invention discloses a combined medicine capable of effectively inhibiting tumor growth and metastasis. The combined drug for resisting tumor growth and metastasis comprises a Rho / ROCK inhibitor and a mitochondrial injury drug, wherein the mitochondrial injury drug is a chemotherapeutic drug coupled with a mitochondrial targeting ligand. The Rhoo / ROCK inhibitor destroys a cytoskeleton structure and blocks cytoskeleton-dependent mitochondrial transfer by inhibiting an Rhoo / ROCK signal channel, and the mitochondrial injury drug can selectively deliver a chemotherapeutic drug to mitochondria to cause mitochondrial injury. Compared with a single drug, the combined drug enhances damage to cytoskeletons and mitochondria, cuts off dynamic energy supply of the mitochondria to the cytoskeletons, makes up for the deficiency of tumor growth inhibition of the Rho / ROCK inhibitor, and has a good curative effect on tumor growth and metastasis resistance.

The invention belongs to the technical field of biological medicine, and discloses a mitochondrial targeting fluorescent probe and a preparation method and application thereof.The mitochondrial targeting fluorescent probe has the structural general formula shown in the formula (I), and the preparation method comprises the step that in the presence of a basic catalyst, the mitochondrial targeting fluorescent probe is prepared. And carrying out Knoevenagel condensation reaction on the nitrogen-containing heterocyclic ring onium iodide and 4-(di (2-chloroethyl) amino)-2-fluorobenzaldehyde, thereby obtaining the compound. The mitochondrial targeting fluorescent probe has mitochondrial targeting, fluorescence imaging capability and antitumor activity, can be used for preparation of tumor cell mitochondrial imaging reagents and antitumor drugs, provides a new strategy for tumor diagnosis and treatment, and has a good application prospect.

The invention discloses a fluorescent probe for high-sensitivity monitoring of cell mitochondrial autophagy as well as a preparation method and application thereof. The structure of the fluorescent probe is as shown in formula I in the specification. According to the fluorescent probe provided by the invention, a phenol group is used as an electron donor and a pH response unit, hydroxyl tricyanopyrrole is used as an electron acceptor, triphenylphosphine is used as a mitochondrial targeting group, fluorine atoms are introduced to a benzene ring to improve the response rate and the fluorescence intensity, and meanwhile, a thiophene ring is introduced to further expand the fluorescence emission wavelength. The probe can rapidly target mitochondria and emit corresponding fluorescence signals based on pH differences of different autophagy stages, so that real-time, high-sensitivity and high-selectivity monitoring of the mitochondria autophagy process is realized. In addition, the fluorescent probe is simple in molecular structure, small in molecular weight and easy to synthesize, purify and subsequently modify. Cell experiment results show that the probe can accurately trace the mitochondrial autophagy process in cells, and has good application potential in the field of biological imaging and detection.

The present invention belongs to the field of biomedicine technology, and discloses an engineered bionic exosome with anti-aging efficacy, a preparation method and application thereof, comprising the following steps: (S1) preparing a bionic cell membrane modified targeting ligand: adding collagen, mitochondrial targeting polypeptide, condensing agent, and 4-dimethylaminopyridine to an organic solvent, activating under a protective atmosphere, and then adding lysine to the above system for reaction to obtain a bionic cell membrane modified targeting ligand; (S2) embedding the bionic cell membrane modified targeting ligand into an engineered exosome bionic membrane, and encapsulating active substances to form an engineered bionic exosome. The exosome of the present invention has better skin permeability, mitochondrial targeting effect, and cell endocytosis effect, has a better protective effect on mitochondria, and can achieve efficient delivery of active substances.