140 results about "Mitochondrial targeting" patented technology

The invention relates to the technical field of pharmaceutical preparations, and discloses a composition with liver protection and antioxidation effects and a preparation method thereof.The composition is prepared from galactosamine modified astragalus membranaceus exosome, schisandra chinensis nanocrystals, liquorice-chitosan nanoparticles, lactobacillus acidophilus freeze-dried powder, beta-(1, 3) / (1, 3, 4-tetramethyl-1, 3-pentanediol monoisobutyrate) sourced from saccharomyces cerevisiae, and beta-(1, 3, 4-tetramethyl-1, 3-pentanediol monoisobutyrate). And (6) a dextran, grape exosome-curcumin hybrid vesicle freeze-dried powder, a curcumin-mitochondrial targeting peptide compound, a resveratrol-hydroxypropyl-beta-cyclodextrin inclusion compound and reduced panthenol. According to the liver-protecting and anti-oxidation composition disclosed by the invention, multi-dimensional intervention on liver protection is realized through an elaborately designed ternary system. The system is composed of a traditional Chinese medicine extract, a microorganism-plant exosome combination and a high-bioavailability antioxidant, all the parts have a synergistic effect, and the liver protection effect is remarkably improved.

The invention discloses a beta-lactoglobulin fluorescent probe as well as a preparation method and application thereof, the structural formula of the probe is (C30H25N4I), the probe is combined with beta-lactoglobulin through non-covalent interaction, a fluorescence signal is triggered to be remarkably enhanced, and low-background fluorescence detection of the beta-lactoglobulin is realized. The probe has low cytotoxicity and excellent mitochondrial targeting ability, and provides a powerful tool for visualization of beta-lactoglobulin in living cells. After being combined with beta-lactoglobulin, a sample can generate obvious color change under the irradiation of an ultraviolet lamp, and the change can be used for evaluating the content of active beta-lactoglobulin in the sample, so that the rapid detection of the milk protein level in a beverage is realized. The beta-lactoglobulin fluorescent probe disclosed by the invention has a wide application prospect in the fields of biological imaging, milk protein detection, beverage quality control and the like.

The invention provides an ovary-targeting ROS response type SS31 nano-particle and a preparation method and application thereof, and belongs to the field of biomedicine.The ovary-targeting ROS response type SS31 nano-particle is constructed by utilizing a porous metal organic framework to carry hyaluronic acid and utilizing an ROS response type thioketone linker to be combined with mitochondrial targeting peptide SS31, and the ovary-targeting ROS response type SS31 nano-particle is used for preparing the ovary-targeting ROS response type SS31 nano-particle. And the SS31 is targeted and delivered to the ovarian tissue. The nanoparticles target ovary and slowly release SS31 under the action of ROS, so that oxidative stress of oocytes and granular cells caused by female reproductive system diseases such as premature ovarian failure, polycystic ovarian syndrome and the like is reduced, and the mitochondrial function of the oocytes and the granular cells is improved; and a foundation is laid for the subsequent clinical treatment of diseases related to the mitochondrial dysfunction of the female reproductive system by using the SS31.

The invention discloses a dual-targeting macrophage membrane nano system and a preparation method and application thereof, and belongs to the field of biological medicine, MKA is wrapped in a macrophage membrane after AuNPs and mitochondrial targeting peptide KLA are connected. The preparation method comprises the following steps: firstly synthesizing AuNPs and modifying, and then wrapping with a macrophage membrane. In treatment, the compound can realize double targeting of tumor cells and mitochondria, enhance radiation-induced DNA damage, inhibit DDR, activate immune pathways and promote T cell infiltration, and can also consume glutathione and amplify oxidative stress to enhance immune effect, and the dose enhancement ratio of the compound in cooperation with 8GyX-rays reaches 3.1. In addition, the MKA is good in biocompatibility in vivo, long in blood circulation time, high in tumor targeting and capable of being quickly cleared through the kidney. The invention provides a new way for tumor radioimmunotherapy, and is helpful for promoting the progress of clinical tumor treatment.

The invention belongs to the technical field of drug system delivery, and particularly relates to a radiotherapy sensitization microneedle and application thereof. The invention relates to a design of a mitochondrial targeting photosensitive microneedle system and an application of the mitochondrial targeting photosensitive microneedle system in melanoma radiosensitization radiotherapy. According to the invention, an intelligent delivery platform of the microneedles (MNs) is fused with mitochondrial targeted photodynamic therapy, and a new normal form is developed for radiosensitization therapy of melanoma. According to the invention, a soluble hyaluronic acid (HA) microneedle array is adopted, and a mitochondrial targeting photosensitizer is loaded. After penetrating through the cuticle, the microneedle is rapidly dissolved in the corium layer, the photosensitizer is released, and mitochondrial targeted enrichment is achieved. Then, under the condition of 660 nm illumination, the photosensitizer generates active oxygen in a mitochondrial matrix to directly destroy the mitochondrial structure and function, so that the mitochondrial ROS storm is induced, and the tumor radiotherapy sensitivity is improved.

The invention provides a combined use of a hyaluronic acid-bilirubin conjugate and honokiol in preparation of a medicine for treating osteoarthritis. The invention provides a honokiol-loaded nanoparticle, which is characterized in that a hyaluronic acid-bilirubin conjugate and honokiol are used as raw materials to prepare a nanoparticle HKL-PNPs, and then the nanoparticle HKL-PNPs is modified by a mitochondrial targeting peptide to obtain the honokiol-loaded nanoparticle HKL-MPNPs. The invention also provides a bionic nanoparticle for treating bone joints. The bionic nanoparticle is prepared by wrapping the honokiol-loaded nanoparticle with an engineered cartilage cell membrane (ECM). According to the invention, cartilage cells with high expression of IL-1R2 are obtained through a gene editing method for the first time, and mitochondrial targeted nanoparticle cores loaded with Sirtuin-3 agonists are coated by cell membranes of the cells, so that the bionic nano preparation is obtained and is used for treating OA. The prepared bionic nano preparation not only can neutralize IL-1beta mediated inflammatory reaction, but also can recover Sirtuin-3 mediated mitochondrial function imbalance, so that the result of synergistic treatment of OA is achieved.

The invention relates to the technical field of biomedical materials, in particular to an alendronate-modified ZIF-8 encapsulated TPP-carbon dot bone targeting nano-material and a preparation method and application thereof.The alendronate-modified ZIF-8 serves as a carrier, TPP-modified carbon dots are encapsulated in the carrier, citric acid, urea and cerium salt serve as raw materials of the TPP-modified carbon dots, and the alendronate-modified ZIF-8 encapsulated TPP-carbon dot bone targeting nano-material is prepared through a one-step method. After cerium-doped carbon quantum dots are synthesized through a hydrothermal method, TPP is grafted through an amide method, alendronate endows the material with a bone targeting function, and TPP modified carbon dots endow the material with antioxidant and mitochondrial targeting functions. According to the invention, the bone targeting property is excellent, and bone focuses are efficiently enriched; the anti-oxidation and anti-inflammatory capacities are high, and the infected microenvironment can be improved; zIF-8 packaging is stable, and functional components can be controllably released; multiple components cooperate to promote bone repair, accumulation of non-target organs is little, and the composition is safe, reliable and suitable for treatment of various bone-related diseases.

The invention discloses a self-assembled PROTAC nano material based on natural product mitochondria targeting, which is a nano particle prepared from mitochondria targeting molecules, a photosensitizer and PROTAC as raw materials through a nano precipitation method. The mitochondrial targeting molecule is berberine, the photosensitizer is hypericin, and the PROTAC is dBET57; the ratio of the amount of substance of the dBET57 to the amount of substance of the berberine is (1-10): (1-10), and the ratio of the amount of substance of the dBET57 to the amount of substance of the hypericin is (1-10): (1-10). The nano material can be used for blocking multiple energy metabolism pathways of tumor cells, including glycolysis and oxidative phosphorylation, meanwhile, mitochondria is damaged, ferroptosis is induced, and the anti-tumor capacity of pharmacodynamic molecules is improved; bRD4 in tumor cells and downstream carcinogenic protein c-Myc of the BRD4 can be efficiently degraded. The invention also discloses an application of the nano material in preparation of antitumor drugs.

The present invention discloses a mitochondrially targeted fluorinated ionizable lipid and its use as a mitochondrial gene delivery vector. A mitochondrially targeted fluorinated ionizable lipid is a compound of formula (I), or a pharmaceutically acceptable salt, stereoisomer, or tautomer thereof, wherein: a = an integer from 2 to 8, b = an integer from 4 to 8; and R1 is an ionizable lipid head group, comprising a mono- or polyamine containing a primary or tertiary amine structure. A mitochondrially targeted lipid carrier comprises the fluorinated ionizable lipid described herein, a cationic lipid, an auxiliary lipid, a structural lipid, and a functional polymer conjugated lipid modified with a mitochondrial targeting group. The fluorinated ionizable lipid gene delivery vector with mitochondrial targeting capability provided by the present invention has excellent biocompatibility, can simultaneously increase the cellular uptake and mitochondrial accumulation of gene drugs, improve the efficiency of mitochondrial gene transfection, and achieve efficient delivery of gene drugs to the mitochondrial matrix. #imgabs0#

The invention discloses a mitochondrial targeting and apoptosis-inducing photosensitive iridium complex and a preparation method thereof, and particularly discloses a 2, 2 '-bipyridine compound, a cyclometalated iridium complex using the compound as a ligand, a preparation method and application of the 2, 2'-bipyridine compound as an antitumor drug. The cyclometalated iridium complex provided by the invention takes a 2, 2 '-bipyridine compound as a ligand, can selectively induce tumor cell apoptosis under an illumination condition, can be used for preparing antitumor drugs, and has a great potential value in the field of photodynamic treatment of tumors.

The application discloses a green preparation method of a medium-chain acylated hydroxytyrosol lipoid-triphenylphosphine derivative and application of the derivative in mitochondrial-targeted antioxidation, and relates to the following steps: taking hydroxytyrosol and saturated halogenated fatty acids with medium-chain length C8-C12 as raw materials, and synthesizing a medium-chain hydroxytyrosol lipoid compound through a low-field ultrasonic coupling immobilized lipase synergistic directional alcohol hydroxyl acylation reaction; and further synthesizing the lipoid compound through high-speed stirring reaction with a triphenylphosphine compound to obtain a medium-chain acylated hydroxytyrosol lipoid-triphenylphosphine derivative. The derivative has the antioxidation effect of mitochondrial-targeted enrichment and active oxygen radical scavenging. The hydroxytyrosol derivative prepared by the application has the characteristics of green efficiency, directional bonding and simple operation. The acylation reaction of hydroxytyrosol does not need to involve flammable and explosive, easily oxidized and corrosive concentrated sulfuric acid and concentrated nitric acid, and the medium-chain acylated hydroxytyrosol lipoid-triphenylphosphine derivative has mitochondrial targeting, can be used as a potential mitochondrial nutritional supplement product, and has a wide application prospect in the health care functions of anti-aging, cardiovascular protection, diabetes prevention and the like.

The invention relates to the technical field of bone regeneration bioactive components, in particular to a monatomic nano-enzyme targeting mitochondria and accelerating bone regeneration and a preparation method thereof.The monatomic nano-enzyme comprises Fe / Cu monatomic double active sites, triphenylphosphine (TPP) molecules targeting the mitochondria and a porous nano-carrier, and the porous nano-carrier is a dendritic mesoporous silica nano-particle (DMSN). The invention effectively overcomes the defects of poor repair effect and long repair period caused by insufficient mitochondrial targeting, low catalytic efficiency and single function in the existing bone regeneration technology.

This invention relates to the field of bionanomaterials technology, and particularly to mitochondrial-targeting nanomaterials capable of releasing HNO, their preparation methods, and applications. The preparation method of the mitochondrial-targeting nanomaterials capable of releasing HNO includes the following steps: synthesis of a silanized HNO donor: hydroxylamine hydrochloride is dissolved in a mixed solvent of anhydrous tetrahydrofuran and anhydrous pyridine, and a dichloromethane solution of 2-(4-chlorosulfonylphenyl)ethyltrimethoxysilane is added. The mixture is stirred at room temperature, and after post-treatment, a silanized HNO donor is obtained; synthesis of aminated carbon dots: chitosan, ethylenediamine, and mercaptosuccinic acid are dispersed in deionized water, and concentrated hydrochloric acid is added and stirred until dissolved. This preparation method can prepare mitochondrial-targeting nanomaterials capable of releasing HNO, achieving precise delivery, controlled release, and visual tracking of HNO to cardiac mitochondria, solving the technical problems of traditional HNO donors' inability to achieve stable loading on carriers and lack of mitochondrial targeting ability.

The present invention provides a D-D-π-A type organic photosensitizer and a synthesis method and application thereof, belonging to the field of photosensitizer and photodynamic therapy application of tumors. The present invention uses triphenylamine and carbazole as electron donors, malononitrile as an electron acceptor, and thiophene as an electron π bridge to synthesize an AIE type photosensitizer with mitochondrial targeting function through a series of simple organic reactions. The AIE type photosensitizer can be used in the synergistic photodynamic and ferroptosis treatment of tumors. The photosensitizer can realize tumor cell imaging and target mitochondria, and can selectively exert photodynamic and ferroptosis effects on tumor cells. It has good therapeutic effect and biosafety, and has strong application prospects in non-invasive tumor treatment.

The invention discloses an organic compound biological probe and a preparation method and application thereof, wherein the organic compound biological probe is In-AIE, and the method comprises: dissolving rutin in acetonitrile, and then adding 4-formyl pyridine and n-tributylphosphine for reflux reaction to obtain compound 1; dissolving 1,1,2-trimethyl-1H-benzo[e]indole and iodoethane in anhydrous acetonitrile for reflux to obtain compound 2; adding 2,4-dihydroxybenzaldehyde and anhydrous potassium carbonate to anhydrous acetonitrile and then dropping 1,4-dibromobutane for reflux reaction to obtain colorless oily compound 3; dissolving compound 2 and compound 3 in anhydrous ethanol for reflux reaction to obtain solid compound 4; dissolving compound 1 and compound 4 in anhydrous acetonitrile for reflux reaction and then purifying to obtain solid In-AIE. It can quickly enter cells within 1 min, has strong anti-photobleaching ability, and integrates multi-channel imaging, pH detection, mitochondrial targeting and photodynamic therapy.

The present invention discloses a dual-targeted macrophage membrane nanosystem, its preparation method and application, which belongs to the field of biomedicine. MKA is formed by connecting AuNPs with the mitochondrial targeting peptide KLA and then encapsulating it in the macrophage membrane. During preparation, AuNPs are first synthesized and modified, and then encapsulated with the macrophage membrane. During treatment, it can dual-target tumor cells and mitochondria, enhance radiation-induced DNA damage, inhibit DDR, activate immune pathways, promote T cell infiltration, and consume glutathione and amplify oxidative stress to enhance immune effects. The dose enhancement ratio synergistic with 8GyX-rays is 3.1. In addition, MKA has good biocompatibility in the body, a long blood circulation time, high tumor targeting, and can be quickly cleared through the kidneys. This invention provides a new approach for tumor radioimmunotherapy and helps promote progress in clinical tumor treatment.

The present invention relates to base editing of mitochondrial DNA. More specifically, the present invention relates to: a method for editing mitochondrial DNA bases by using a mitochondrial targeting sequence (MTS); and a base editing system used in the method. The base editing system comprises a DNA binding protein and a deaminase, or polynucleotides encoding these, and does not include an affinity tag or a polynucleotide encoding same. The present invention is useful for editing mitochondrial DNA bases of animal cells or plant cells.

The invention discloses a platinum (IV)-internal peroxy prodrug molecule and application thereof, and belongs to the technical field of biological medicine. The series of molecules are characterized by being Pt (IV) complexes modified by internal peroxide and mitochondrial targeting groups. The synthesis method sequentially comprises the following steps: carrying out oxidation and hydroxyl modification on a platinum drug, introducing a mitochondrial targeting group and a singlet oxygen carrier through an esterification reaction, and carrying out a photosensitive reaction to prepare the endoperoxide. The series of molecules are reduced into Pt (II) drugs in a tumor microenvironment, singlet oxygen is released at the same time, a mitochondrial targeting group can accurately deliver the treatment molecules, and cancers are efficiently treated. A representative molecule Oxa-EM is selected for instance analysis, the molecule generates a Pt (II) complex with anticancer activity while releasing singlet oxygen, and the singlet oxygen-Pt combined anticancer purpose is achieved. Probes DPBF and SOSG are selected to prove that the molecule releases singlet oxygen, and high performance liquid chromatography is utilized to successfully prove that the Pt (II) complex is released in a reducing environment. Further cell and animal level experiments verify that the molecule has excellent anti-tumor efficacy and safety. The invention discloses design, synthesis and biological test of platinum (IV)-peroxy prodrug molecules, and the platinum (IV)-peroxy prodrug molecules have a wide anticancer application prospect.

The invention belongs to the field of biological nano medicines, and particularly relates to a conductive polymer nano enzyme as well as a preparation method and application thereof. According to the conducting polymer nano-enzyme, polyvinyl alcohol, polypyrrole and polythiophene serve as basic frameworks, different metal ions are coordinated, a conducting polymer nano-enzyme library is constructed, ruthenium-based polymer nano-enzyme Ruzyme is screened out through a series of enzyme activity characterization, the Ruzyme is subjected to mitochondrial targeting modification to obtain ICT-Ruzyme, and the ICT-Ruzyme is subjected to myocardial targeting peptide modification to obtain CICT-Ruzyme. The conductive polymer nano-enzyme prepared by the invention is uniform in size, not only has relatively good conductivity and stability, but also has excellent active oxygen and active nitrogen removal capability, and the conductive polymer nano-enzyme modified by mitochondrial targeting and myocardial targeting peptides can more effectively target myocardial cells, so that the treatment effect is improved. And Fe < 2 + > accumulation and lipid peroxidation which are related to myocardial cell ferroptosis induced by lipopolysaccharide are reduced, so that sepsis cardiomyopathy is effectively relieved.

This application discloses a preparation method and application of injectable hydrogel microspheres. By integrating the hydrogel drug delivery system with liposome microparticles obtained by microfluidic technology, NMN is encapsulated by liposome material, and the mitochondrial targeting peptide SS-31 is introduced into the liposome to achieve a more sustained and stable release, thereby enabling NMN to selectively target cell mitochondria and improve the application effect.

The invention belongs to the technical field of biological medicine, and discloses a mitochondrial targeting fluorescent probe and a preparation method and application thereof.The mitochondrial targeting fluorescent probe has the structural general formula shown in the formula (I), and the preparation method comprises the step that in the presence of a basic catalyst, the mitochondrial targeting fluorescent probe is prepared. And carrying out Knoevenagel condensation reaction on the nitrogen-containing heterocyclic ring onium iodide and 4-(di (2-chloroethyl) amino)-2-fluorobenzaldehyde, thereby obtaining the compound. The mitochondrial targeting fluorescent probe has mitochondrial targeting, fluorescence imaging capability and antitumor activity, can be used for preparation of tumor cell mitochondrial imaging reagents and antitumor drugs, provides a new strategy for tumor diagnosis and treatment, and has a good application prospect.

The invention discloses a fluorescent probe for high-sensitivity monitoring of cell mitochondrial autophagy as well as a preparation method and application thereof. The structure of the fluorescent probe is as shown in formula I in the specification. According to the fluorescent probe provided by the invention, a phenol group is used as an electron donor and a pH response unit, hydroxyl tricyanopyrrole is used as an electron acceptor, triphenylphosphine is used as a mitochondrial targeting group, fluorine atoms are introduced to a benzene ring to improve the response rate and the fluorescence intensity, and meanwhile, a thiophene ring is introduced to further expand the fluorescence emission wavelength. The probe can rapidly target mitochondria and emit corresponding fluorescence signals based on pH differences of different autophagy stages, so that real-time, high-sensitivity and high-selectivity monitoring of the mitochondria autophagy process is realized. In addition, the fluorescent probe is simple in molecular structure, small in molecular weight and easy to synthesize, purify and subsequently modify. Cell experiment results show that the probe can accurately trace the mitochondrial autophagy process in cells, and has good application potential in the field of biological imaging and detection.

The present invention belongs to the field of biomedicine technology, and discloses an engineered bionic exosome with anti-aging efficacy, a preparation method and application thereof, comprising the following steps: (S1) preparing a bionic cell membrane modified targeting ligand: adding collagen, mitochondrial targeting polypeptide, condensing agent, and 4-dimethylaminopyridine to an organic solvent, activating under a protective atmosphere, and then adding lysine to the above system for reaction to obtain a bionic cell membrane modified targeting ligand; (S2) embedding the bionic cell membrane modified targeting ligand into an engineered exosome bionic membrane, and encapsulating active substances to form an engineered bionic exosome. The exosome of the present invention has better skin permeability, mitochondrial targeting effect, and cell endocytosis effect, has a better protective effect on mitochondria, and can achieve efficient delivery of active substances.

The present invention discloses a fluorescent probe and its preparation method and application. The fluorescent probe comprises two parts, namely 5-iodo-2'-deoxy-2'-fluorouridine and 2-(4-ethynylphenyl)-1,2,2-triphenylethylene. Due to the 5-iodo-2'-deoxy-2'-fluorouridine unit, the biocompatibility of the fluorescent probe is improved; due to the 2-(4-ethynylphenyl)-1,2,2-triphenylethylene group, the fluorescent probe has high fluorescence intensity. The design of the fluorescent probe in the present invention well solves the problems of biocompatibility and fluorescence yield, and has multiple functions such as mitochondrial targeting, pH detection, and mercury ion recognition. The preparation method of the fluorescent probe of the present invention is simple and efficient. The fluorescent probe of the present invention has good selectivity, high biocompatibility, and convenient use of related instruments, and is a detection means with relatively high sensitivity in current chemiluminescence detection methods, and has important potential application value.

The invention discloses a mitochondrial targeting pyridinium cyanine photosensitizer compound, a preparation method, a compound and application thereof, and the pyridinium cyanine photosensitizer compound is generated through organic reaction by taking N-methyl-N-hydroxyethyl naphthylamine as an electron donor, pyridinium cyanine as an electron acceptor and double bonds as pi bridge connection. After the pyridycyanine photosensitizer compound is self-assembled and combined with HSA, specific fluorescent staining of mitochondria is achieved, active oxygen is efficiently generated under white light irradiation and directly acts on the mitochondria, and the cancer cell killing efficiency is improved. The preparation method is simple and efficient, and the prepared photosensitizer compound has a good photodynamic therapy effect and biological safety and has a good application prospect.

The present invention discloses a targeted photosensitizer, FA-BAT-NPs, and its preparation and application. First, the invention chemically links the photosensitizer 5-ALA with the mitochondrial targeting group TPP and modifies the Boc group to increase lipid solubility, thereby preparing a novel mitochondrial-targeted photosensitizer, BAT. The BAT is then prepared into BAT-NPs (nanoparticles) using an emulsification dispersion method. Finally, FA is modified onto the surface of the BAT-NPs using PEG as a connecting linker via an amide reaction, yielding the targeted photosensitizer, FA-BAT-NPs. The targeted photosensitizer, FA-BAT-NPs, possesses good water solubility and sustained release in vivo, exhibiting a strong inhibitory effect on breast cancer and producing virtually no toxic side effects.

The invention discloses a nano preparation with mitochondrial targeting, a preparation method of the nano preparation and application of the nano preparation in myasthenia gravis. According to the method, a nano-carrier is prepared from PLGA and HPMC through an anti-solvent precipitation method, astragaloside and black phosphorus quantum dots are loaded in a manner of forming nanoparticles, and the surfaces of the nanoparticles are modified with mitochondrial targeting micromolecules NH2-PEG-TPP, so that the nano-preparation for mitochondrial targeting treatment of myasthenia gravis is obtained. The nano preparation has a mitochondrial targeting function, can improve the distribution of drugs in muscle cell mitochondria, avoids the too fast degradation of BPQDs in vivo, improves the bioavailability of AS-IV and BPQDs, exerts the function of resisting oxidative stress to protect mitochondria, and provides a nano preparation for effectively treating mitochondrial damage for myasthenia gravis.

The invention belongs to the field of fluorescent dyes and biological imaging, and particularly provides a mitochondrial targeting fluorescent dye as well as synthesis and application thereof. The structural formula of the mitochondrial targeting fluorescent dye is shown as a formula (I). The compound is synthesized by one-step heating reaction of benzothiazole-2-acetonitrile and Fisher's aldehyde under the action of alkali. The mitochondrial targeting fluorescent dye has good cell membrane penetrability, can be rapidly taken by cells, and is good in mitochondrial targeting, high in light stability and low in toxicity to the cells. Meanwhile, the preparation process is simple, the cost is low, clear and long-time fluorescence imaging analysis and dynamic fluorescence monitoring on mitochondria in cells can be realized, and the fluorescent probe has a good application prospect.

The invention relates to a covalent mitochondrial fluorescent probe as well as a preparation method and application thereof, the covalent fluorescent probe takes Cy3 or Cy5 with low phototoxicity as a fluorophore and chloroacetyl chloride as a covalent group, the Cy3 or Cy5 is also used as a mitochondrial targeting group, and the chloroacetyl chloride is used as a covalent group. The covalent group chloroacetyl chloride on the fluorescent probe molecule is covalently bound with the mitochondrial inner membrane protein to realize targeted covalent labeling, and compared with other fluorescent probes synthesized by covalent groups, the covalent fluorescent probe has more excellent labeling stability, has good labeling stability, strong light stability and low phototoxicity, and can be used for detecting the mitochondrial inner membrane protein of the mitochondrial inner membrane protein of the mitochondrial inner membrane protein of the mitochondrial inner membrane protein. And the requirements of multicolor imaging and long-term dynamic imaging of mitochondria can be met.

This invention relates to the field of optical sensing and imaging detection technology, specifically to a triphenylene-oxanthracene derivative, its preparation method, and a fluorescent probe for viscosity detection. The triphenylene-oxanthracene derivative has the general formula shown in Formula I. Compared to Rhodamine B, the synthesized triphenylene-oxanthracene derivative exhibits a larger Stokes shift. Furthermore, the fluorescence intensity of the triphenylene-oxanthracene derivative increases with increasing cell viscosity. In addition, the triphenylene-oxanthracene derivative can be further used to detect mitochondrial viscosity, exhibiting good mitochondrial targeting. In summary, the probe improves the Stokes shift, has a wide optical response pH range, and possesses excellent mitochondrial targeting.