331 results about "Biological imaging" patented technology

The invention belongs to the technical field of quantum and biological imaging, and particularly relates to an oral cavity biological imaging device based on a quantum entanglement phase. According to the invention, energy-time entangled photon pairs are generated by using a continuous laser pumping nonlinear waveguide, and signal photons are separated from idler frequency photons through an optical filter; a to-be-measured oral biological sample is placed in the unbalanced interferometer, two interference curves of different phases are obtained by measuring Franson interference between signal photons and idler frequency photons when the biological sample exists or not, and the entanglement phase difference of the two interference curves is calculated. The position of the sample to be detected is changed through the two-dimensional scanning platform, Franson interference is executed, and entanglement phase differences of different positions are obtained. And combining the phase differences to obtain a quantum phase image of the sample. According to the invention, high-precision imaging of a microstructure can be realized; according to the invention, each component can be from a mature photoelectric device, the device has the advantages of miniaturization, integration, practicability and the like, and a new way is opened up for quantum precision measurement and life science development.

The invention relates to the field of biological imaging, and particularly discloses an in-vitro cell microenvironment dynamic monitoring method for two-photon frequency domain fluorescence lifetime imaging, which comprises the following steps: S1, by using a two-photon frequency domain fluorescence lifetime imaging TP-FD-FLIM technology, losslessly representing microenvironment change through fluorescence lifetime parameters, and monitoring oxidative stress reaction and neutrophil migration behavior in real time, analyzing the effect of the associated compound in combination with an in-vitro cell model; and S2, based on the physical basis of FD-FLIM, establishing a frequency domain fluorescence lifetime signal processing and analysis system under periodic excitation, analyzing the dynamic correlation characteristics of the phase delay phi and the modulation depth M of a fluorescence signal relative to exciting light, and carrying out quantitative backstepping on molecular lifetime parameters. According to the in-vitro cell microenvironment dynamic monitoring method for two-photon frequency domain fluorescence lifetime imaging provided by the invention, neuroinflammatory response can be observed in a lossless and real-time manner on the subcellular scale.

System and methods for optical coherence tomography. The systems may include a source of optical radiation, an optical fiber, a detector, an interface medium, a mirror in the embedding medium, and a processor. The optical fiber may provide a common path for optical radiation reflected from a reference and a target. The detector may receive the optical radiation reflected from the reference and the target. The interface medium may be at the reference interface and in optical contact with the distal end of the optical fiber. The mirror may include a silicon die having a reflective coating. The processor may generate an image of the target based upon the optical radiation received by the detector.

The invention provides a near-infrared fluorescent material activated by Sm < 2 + > as well as a preparation method and application of the near-infrared fluorescent material. The chemical composition of the fluorescent material is Si < 2-x > Al < x > N < 2 + y > O < 1-y >: Mz, Smm, Lnn, m in the formula is one or more elements of Ba, Sr, Ca and Mg; ln is one or more elements of Bi, Yb, Eu, Nd, Gd, Tb, Dy, Ho, Er, Tm and Pr; 0 < = x < = 0.2, 0.1 < = y < = 0.5, 0 < = z < = 0.5, 0 < = m < = 0.15, and 0 < = n < = 0.1. The luminescent material provided by the invention has a brand new chemical composition, takes Sm < 2 + > as an activation center, and shows a near-infrared luminescence behavior in a gap occupation manner. The fluorescent material can be excited by light within the wavelength range of 300-600 nm, so that narrow-band near-infrared light of 681 nm is emitted, and therefore, the fluorescent material can convert ultraviolet light, purple blue light and green light into near-infrared light. The near-infrared fluorescent material provided by the invention has the advantages of convenient synthesis method and stable chemical properties, and is suitable for the fields of biological imaging, plant growth illumination, white light LEDs, temperature sensors and the like.

The present invention relates to a microelectrode array device and method for biological imaging comprising a well for containing a liquid medium; an optically transparent substrate; and a microelectrode array comprising multiple electrode pads. Each electrode pad comprises a conductive film having a thickness between 5 nm and 1000 nm comprising a carbon nanotube network and / or graphene. The microelectrode array further comprises at least one counter electrode and electrically conductive traces connecting the electrode pads with a voltage source and connecting the at least one counter electrode with the voltage source such that a potential difference can be provided between the electrode pads and the counter electrode.

The invention discloses a rapid quantitative differential interference microscopy system and method based on holographic recording. The system comprises a partially coherent illumination module, and a microscope objective, a tube lens, a transmission grating, a first linear polarizer, a first thin lens, a half-wave plate, a shading plate, a phase-type spatial light modulator, a second thin lens, a second linear polarizer and an image acquisition module which are sequentially arranged along the optical axis direction of the partially coherent illumination module. According to the invention, the phase-type spatial light modulator is utilized to realize shearing offset in any direction and at any degree, so that the device is suitable for different types of application occasions; the system realizes holographic recording of differential interference by utilizing grating carrier frequency, realizes quantitative differential interference detection on a to-be-detected sample only by utilizing two acquired images, has very high time resolution, spatial resolution and imaging precision, and has a wide application prospect in biological imaging and industrial detection.

The invention belongs to the technical field of carbon material luminescent materials, and particularly relates to a preparation method of a multicolor luminescent carbon dot and bioimaging application of the multicolor luminescent carbon dot. The invention provides a simple and controllable multicolor luminescent carbon dot and a preparation method thereof. By adjusting the reaction time in the heat treatment process of o-phenylenediamine and thiourea solvents, various luminescent carbon dots, including green light, yellow light and red light carbon dots, are successfully separated. The carbon dot provided by the invention can realize controllable adjustment of fluorescence performance by regulating and controlling the conjugation degree of the carbon core and the content of graphite nitrogen. According to the method, the fluorescence mechanism in the carbon dot forming process is understood more deeply, and guidance is provided for designing the structure and optical performance of the carbon dots and achieving controllable synthesis of the high-performance carbon dots. The multicolor luminescent carbon dot provided by the invention has excellent optical performance, stable morphological structure and low cytotoxicity, and has a good application prospect in biological imaging.

The invention discloses a super-resolution structured light reconstruction method based on a wavelet enhanced CNN-Transformer structure. The super-resolution structured light reconstruction method comprises the following steps of: firstly, carrying out wavelet enhancement on a CNN-Transformer structure; according to the method, image reconstruction is realized based on a WECT network adopting a coding-decoding architecture; the method comprises the following steps: firstly, extracting initial features through shallow convolution, performing two-stage down-sampling by using discrete wavelet transform, and respectively inputting results into a convolutional neural network and a Swin Transform module to perform parallel feature extraction and fusion; secondly, performing two-stage up-sampling through inverse discrete wavelet transform, and performing jump connection with shallow layer features; finally, pixel shuffling up-sampling operation is introduced, and residual jump connection between the input image and the output image is established; according to the method, high-resolution details can be efficiently recovered while the structure reduction precision is ensured, the image definition and contrast are enhanced, and the application value and practicability of the SIM image in scenes such as biological imaging and tissue analysis are improved.

The invention provides a novel D-A-D type fluorescent probe material as well as a preparation method and application thereof. The fluorescent probe material has good stability, the preparation process is simple, and the synthesis condition is mild. Moreover, the D-A-D type fluorescent probe material has good solubility and stability, and a complex of the D-A-D type fluorescent probe material and zinc ions can identify pyrophosphate ions with high selectivity and high sensitivity. The interference of phosphate ions, hydrogen phosphate ions, dihydrogen phosphate ions, fluorine ions, chloride ions, nitrate ions, acetate ions, sulfate ions and carbonate ions is avoided; after the zinc ion complex of the D-A-D type fluorescent probe material is combined with PPi, the fluorescence intensity is remarkably enhanced, the D-A-D type fluorescent probe material has a higher signal-to-noise ratio, in-situ imaging detection of a target object in living cells or tissues is realized, the defects of a traditional molecule and ion detection method are overcome, and the D-A-D type fluorescent probe material is suitable for being widely applied to the field of biological imaging. # imgabs0 #

The invention relates to a nitrogen-containing bowl-shaped conjugated pH-responsive fluorescent probe and a preparation method and application thereof, the nitrogen-containing bowl-shaped conjugated pH-responsive fluorescent probe is characterized in that the structural formula of the nitrogen-containing bowl-shaped conjugated pH-responsive fluorescent probe is # imgabs0 #, and R is hydrogen, acetyl or p-toluenesulfonyl. Compared with the prior art, the nitrogen-containing bowl-shaped conjugated molecule provided by the invention shows a highly sensitive response characteristic in an acid-base medium. Due to the introduction of nitrogen atoms, the electronic structure of molecules is obviously changed, lone pair electrons of the nitrogen atoms can participate in the reaction, and the protonation behavior is obvious under the acidic condition. After protonation, the fluorescence intensity of the molecule is obviously enhanced, and the emission wavelength has obvious red shift, so that the probe has important application value in the aspect of developing a high-sensitivity acid-base sensor. In addition, the nitrogen-containing polycyclic aromatic hydrocarbon compound also shows application prospects in the fields of organic light-emitting diodes, information safety, biological imaging and the like.

The invention discloses a para-substituted heptamethine quinone cyanine dye as well as a synthesis method and application thereof, the structural general formula of the para-substituted heptamethine quinone cyanine dye is shown as I, that is, by introducing a specific quinone structure and a substituent group, the near-infrared light absorption and triplet excited state generation capability is optimized. The dye efficiently absorbs light within the wavelength range of 600-700 nanometers, the intersystem crossing efficiency is remarkably improved, and the photo-initiation effect is enhanced. According to the dye, heavy atoms are not used, and the solubility and the environmental protection property are improved. The synthesis method is simple, low in cost, mild in condition and suitable for large-scale production. The key steps comprise formation of quaternary ammonium salt, preparation of a condensing agent and synthesis of a final product, and parameters of each step are optimized to ensure high yield. The dye is suitable for photodynamic therapy, photoinitiation of photopolymerization materials, biological imaging and the like, and has a wide application prospect.

The invention discloses an aza-BODIPY biomedical phototherapy agent taking oxajulolidine as a donor and a preparation method thereof, and relates to a medical phototherapy agent for treating brain glioma and a preparation method thereof, and the prepared compound is prepared by introducing an oxajulolidine fragment with electron donating property into a key position of an aza-BODIPY skeleton, so that the aza-BODIPY biomedical phototherapy agent is obtained. The donor-acceptor type molecular near-infrared phototherapy agent with a new structure is constructed, has good near-infrared absorption and emission performance and excellent stability, and shows a remarkable effect in the field of cancer photodynamic (PDT)-photothermal (PTT) synergistic treatment. The structural categories of the near-infrared aza-BODIPY biomolecules are enriched, and a new molecular platform is provided for expansion and application of the near-infrared aza-BODIPY biomolecules in the fields of biological imaging, tumor diagnosis and treatment, functional materials and the like.

According to the super-resolution polarized light acoustic imaging device and method, galvanometer scanning is achieved on the surface of a sample, excitation light is modulated in different linear polarization directions on each scanning point on the surface of the sample, and the intensity of photoacoustic signals generated by the sample under the excitation light in the different linear polarization directions is obtained through measurement; an initial photoacoustic image of a sample surface structure is constructed by utilizing photoacoustic signal intensities under exciting light in different linear polarization directions, and the initial photoacoustic image is subjected to phase-locked amplification and three-dimensional deconvolution processing, so that a super-resolution photoacoustic image in each linear polarization state is obtained. Compared with traditional polarized light acoustic imaging, the method has the advantages that the diffraction limit of traditional photoacoustic imaging can be overcome, super-resolution imaging of the surface structure of the sample can be achieved while unmarked imaging is achieved, and finer expression of the structure of the sample is achieved. The method has potential application prospects in the fields of biological imaging and biological tissue surface structure conformation detection.

The invention relates to the technical field of biological imaging, in particular to a three-dimensional living body imaging system and method, and the system comprises an excitation device which is used for emitting a first parallel Gaussian light spot; the near-infrared structured light generation device is used for modulating the first parallel Gaussian light spot into first dynamic structured light; the first deformable mirror is used for reflecting a structural fluorescence signal in a living body to be imaged to the first dichroscope; the first dichroscope is used for dividing the structure fluorescence signal, reflected by the first deformable mirror, in the to-be-imaged living body into a first path of light and a second path of light; the near-infrared adaptive optical device is used for obtaining aberration according to the first path of light and a specified light structure; the imaging device is used for acquiring a two-dimensional image corresponding to the living body to be imaged based on the second path of light; and the three-dimensional reconstruction device is used for reconstructing the two-dimensional image into a three-dimensional model and controlling the first deformable mirror according to the aberration, so that the aberration between the first path of light and the specified light structure is reduced within a preset range.

The invention discloses a near-infrared fluorescent probe based on a xanthene-quinoline conjugated skeleton for detecting Cu < 2 + > as well as a preparation method and bioimaging application of the near-infrared fluorescent probe. The molecular formula of the fluorescent probe is C34H32N4O2. Based on the strategy of inhibiting the PET process, the large Stokes shift near-infrared fluorescent probe for specifically recognizing Cu < 2 + > is constructed, and spectrum experiments show that the probe has excellent recognition performance on Cu < 2 + >, the emission wavelength is 720 nm, and the Stokes shift reaches 170 nm; (2) the specificity is high, and Cu < 2 + > and other biological related metal ions can be effectively distinguished; (3) low detection limit (LOD = 17.6 nM); (4) quick response dynamics (lt; 30s) for a motor vehicle. More importantly, the probe XQ-NH2 is successfully applied to Cu < 2 + > imaging in living cells and living mice, it is proved that XQ-NH2 not only can provide a reliable technical means for Cu < 2 + > detection and imaging research in a complex biological system, but also shows that XQ-NH2 has important application potential in related fields.

The invention belongs to the technical field of fluorescent polymer materials, and particularly discloses bifuran polyamide with high quantum yield and capable of emitting yellow-green wavelength light as well as a preparation method and application of the bifuran polyamide. The method comprises the following steps: dissolving a bifuranoyl chloride monomer in an organic solvent to obtain an organic phase; adding an alkaline aqueous solution into the organic phase to form a phase interface, then adding a diamine monomer, and stirring to carry out an interfacial polymerization reaction; and after the reaction is finished, filtering, precipitating, washing and drying to obtain the bifuran polyamide. The bifuran polyamide is prepared by regulating and controlling the main chain structure and chain rigidity and flexibility of the polymer through a diamine monomer with a specific molecular structure, the emission wavelength of the amorphous bifuran polyamide can reach 540-552 nm under exciting light of 480 nm, red shift is performed to 565-568 nm after crystallization, long-wavelength yellow-green light emission, high-quantum-yield light emission and good biocompatibility are achieved, and the bifuran polyamide can be applied to the field of organic light emitting devices, such as organic light emitting devices, organic light emitting devices, organic light emitting devices, organic light emitting devices, organic light emitting devices, organic light emitting devices, organic light emitting devices and the like. The application of the bio-based polyamide in the field of biological imaging is expanded.

The invention provides a halogenated polysulfide aromatic hydrocarbon compound as well as a preparation method and application thereof. The structural formula of the halogenated polysulfide aromatic hydrocarbon compound is as shown in formula (I). Wherein X is one of F, Cl and Br. The halogenated polysulfide aromatic hydrocarbon compound disclosed by the invention can be used as a photoluminescent material and is a photostimulation response type luminescent material. The molecular structural formula of the photoluminescent material is as shown in a formula (1), and in the molecular structural formula of the photoluminescent material, based on a hexasulfobenzene unit as a core skeleton, the material is endowed with a unique photostimulation response characteristic through specific chemical group modification. The innovative material not only shows excellent light response capability, but also has the characteristic of aggregation-induced emission enhancement, so that the material has potential application value in the fields of biological imaging technology, organic light-emitting material application and the like.

The invention provides a body type organic silicon macromolecular azide coupling agent and a preparation method thereof. According to the invention, a macromolecular azide coupling agent with excellent performance is developed through molecular design and combination of cross-shaped and dendritic organic silicon structures. The coupling agent can be efficiently decomposed under ultraviolet irradiation and realize crosslinking of materials, and has the following advantages: (1) efficient crosslinking: uniform distribution of azide groups improves the uniformity and density of crosslinking; (2) safety and stability: the macromolecular structure significantly improves the storage stability of the material, and reduces the toxicity and explosion risk; and (3) multi-functional adaptation: due to the structural flexibility, the method is suitable for multiple fields such as photoresist, liquid crystal displays and biological imaging, and has a wide application prospect.

The invention provides a diketopyrrolopyrrole / cadmium quantum dot composite material as well as a preparation method and application thereof. The composite material comprises a composite material system formed by compounding diketopyrrolopyrrole derivative molecules and cadmium quantum dots. According to the invention, the rate and direction of fluorescence resonance energy transfer (FRET) between organic components and inorganic components in the composite material are simply and efficiently regulated and controlled by controlling the size of the quantum dots. The result of the embodiment shows that the composite material with the FRET rate of about 10 < 10 >-10 < 11 > s <-1 > can be obtained by adopting the method disclosed by the invention, and the energy transfer direction of FRET can be accurately regulated and controlled, so that energy transfer from ligand molecules to quantum dots or from quantum dots to ligand molecules is realized. The method is simple and convenient to operate and implement, and an inorganic-organic composite material system with broad-spectrum response can be constructed so as to realize efficient conversion and utilization of solar energy. In addition, the invention also provides a new thought for research and development in the fields of biological imaging, photoelectric devices and the like adopting a quantum dot-dye molecule composite material system.

The invention discloses perovskite nanoparticle ink for ink-jet printing and application of the perovskite nanoparticle ink, and belongs to the technical field of electronic printing. The solute of the ink is perovskite nano-particles with anisotropic shapes or is formed by mixing the perovskite nano-particles with the isotropic shapes and the perovskite nano-particles with the anisotropic shapes. The solute can be stably dispersed in a solvent, the migration behavior of the solute in the deposition process is effectively controlled based on introduction of shape anisotropic nanoparticles, and the coffee ring effect is remarkably inhibited, so that a uniform, low-roughness and high-quality perovskite thin film is obtained, and the application requirements of high-performance electroluminescent devices can be met; and under the condition that the perovskite nanoparticles with anisotropic shapes and linear polarization are orderly deposited and arranged in the film forming process, the prepared perovskite thin film also has linear polarization, can be used for preparing linear polarization electroluminescent devices, and shows excellent application prospects in the fields of radiation measurement, biological imaging, three-dimensional display and the like.

The biological imaging analysis device includes a staining module configured to stain cells of a biological sample so as to generate stained cells. The present analysis device also includes an illumination module configured to illuminate the stained cells, and the illumination module includes a plurality of pulsed lights. The present analysis device further includes an imaging module configured to capture an image of the stained cells. A method for flow imaging of a biological sample includes flowing a biological sample containing stained cells through an image capture region of a flow cell. The method also includes illuminating the stained cells in the image capture region with a plurality of pulsed lights using the illumination module. The method further includes capturing an image of the stained cells in the image capture region with the imaging module.

There is provided a bioimaging system for determining a spatial distribution of one or more physical properties within a biological structure, the system comprising: a data input configured to receive a plurality of measurements related to one or more physical fields; and a processing device configured to determine a spatial distribution of one or more physical properties within the biological structure using the plurality of measurements. The processing device forms a discretized full order model of the biological structure, describes a baseline spatial distribution of the biological structure using the model, and determines optimized values of coefficients describing the baseline spatial distribution based on the plurality of measurements. The processing device also forms a hybrid model comprising baseline spatial distribution coefficients and a set of deviation full-order model coefficients describing deviations relative to the baseline spatial distribution, determines optimized values for the baseline spatial distribution coefficients and the deviation full-order model coefficients of the hybrid model, and determining a spatial distribution of one or more physical properties within the biological structure using the optimized values of the coefficients.

The invention discloses a zebra fish imaging system, and belongs to the technical field of biological imaging detection, the zebra fish imaging system comprises a stirring module and an imaging module, the stirring module comprises a stirring mechanism, the stirring mechanism comprises a hollow stirring shaft, the upper end of the hollow stirring shaft is connected with a sample loading pipe, and the lower end of the stirring shaft is provided with a sample loading bin; the imaging module comprises a first platform, an imaging area is arranged on the first platform, a detection tube is arranged in the imaging area, the sample loading tube is connected in series with the detection tube, a rotary driving device is arranged in the imaging area along the axial direction of the detection tube, and a detection positioning module is arranged in the imaging area along the axial direction vertical to the detection tube. According to the scheme, no-dead-corner observation of the zebra fish is achieved, the imaging efficiency and imaging quality of the zebra fish are greatly improved, continuous detection is achieved, and a methodological tool is provided for medicine screening and development research of the zebra fish.

The invention relates to development of novel red light and dark red light emitting Nile red derivatives and application of the novel red light and dark red light emitting Nile red derivatives in cell lipid droplet fluorescence imaging, and belongs to the technical field of biological imaging. Chemical names of the fluorescent probes are respectively 9-(4-(diethylamino) phenyl)-5H-benzo [a] phenoxazine-5-ketone, (E)-9-(4-(dimethylamino) styryl)-5H-benzo [a] phenoxazine-5-ketone, (E)-9-(4-(-1-pyrrolidinyl) styryl)-5H-benzo [a] phenoxazine-5-ketone and (E)-9-(4-(diethylamino) styryl)-5H-benzo [a] The invention relates to phenoxazine-5-ketone. The series of cell lipid droplet fluorescence imaging probe molecules prepared by the invention are multifunctional fluorescence imaging probe molecules which have ICT emission characteristics, high labeling selectivity, high light stability, environmental sensitivity and excellent biocompatibility and can be used for confocal imaging.

The invention discloses a method for preparing a one-dimensional organic metal halide single crystal through a solvent regulation and control method and application of the one-dimensional organic metal halide single crystal. According to the method, BBI-ZnCl2 and BBI-ZnCl4 single crystals are respectively prepared by taking bibenzimidazole and ZnCl2 as raw materials and adjusting the volume ratio of a methanol / water mixed solvent, the process is simple, the condition is mild, and complex equipment is not needed. After the obtained single crystal is compounded with polymethyl methacrylate, a material with excellent room-temperature phosphorescence performance is formed, and continuous afterglow emission can be generated under excitation of 365 nm ultraviolet light. By regulating and controlling the light-emitting characteristic difference of BBI-ZnCl2 and BBI-ZnCl4, the material can realize dynamic information hiding and display (such as pattern switching after ultraviolet irradiation / closing), and has outstanding advantages in the field of encryption and anti-counterfeiting. In addition, the material has the characteristics of cheap raw materials, good stability and no toxicity, and is also suitable for the fields of fire safety, biological imaging and the like. The preparation method has the innovativeness of the synthesis method and the practicability of the functional material, and has a wide industrialization prospect.

A biological imaging system for determining a spatial distribution of one or more physical properties within a biological structure is provided, comprising a data input arranged to receive a plurality of measurements relating to one or more physical fields and a processing device arranged to determine a spatial distribution of one or more physical properties within the biological structure using said plurality of measurements. The processing device forms a discretized full order model of the biological structure, uses this model to describe a baseline spatial distribution of the biological structure and determines optimised values for the coefficients describing the baseline spatial distribution based on the plurality of measurements. The processing device also forms a hybrid model comprising the baseline spatial distribution coefficients and a set of deviation full order model coefficients which describe a deviation from the baseline spatial distribution, determines optimised values for the baseline spatial distribution coefficients and the deviation full order model coefficients of the hybrid model and determines the spatial distribution of one or more physical properties within the biological structure using optimised values of the coefficients.

The present invention discloses a class of quinoacridine-5,9-diketone MR-TADF red light emitting materials and their preparation methods and applications, belonging to the technical field of organic photoelectric materials. The molecules of this type of material promote the minimization of bonding / antibonding of frontier molecular orbitals through the resonance effect generated by electron-deficient nitrogen / carbonyl and electron-rich oxygen / nitrogen. In addition, the rigid conjugated skeleton suppresses the structural relaxation of the molecular excited state and the vibration coupling between the excited state and the ground state, thereby obtaining a red light emitting material with a narrow half-width (<70nm) and high luminous efficiency; the introduction of a tert-butyl group on the periphery of the molecule can reduce the reorganization energy of the molecule; grafting different substituent units can regulate the stacking and film-forming properties of the molecule. The maximum emission peak of the obtained doped red light electroluminescent device is located at 601 nm, the half-width is 68 nm, and the corresponding maximum external quantum efficiency is 9.38%, which has a wide range of applications in the fields of organic electroluminescent displays and biological imaging.

The invention discloses a fluorescent pigment compound as well as a preparation method and application thereof. The chemical structural formula of the fluorescent pigment compound is shown in the specification. Wherein n is an integer from 2 to 6, and R1 and R2 are respectively and independently selected from C1-C3 alkyl groups. By introducing 4-tert-butylphenyl and tert-butylsilane ether chains, the compound has excellent solubility, film-forming property, high fluorescence quantum yield (gt, 95%) and light stability (retention rate gt, 90% after ultraviolet irradiation for 500 hours). The preparation method is simple, is suitable for a solution processing technology, and can be widely applied to the fields of OLEDs, light conversion adhesive films, fluorescence sensing, biological imaging and the like.

This invention relates to ONOO ‑ In the field of detection technology, specifically involving an ONOO ‑ Near-infrared fluorescent probe, its preparation method, and its application. This invention constructs a novel fluorescent probe specifically for detecting ONOO. ‑ The ONOO ‑ Near-infrared fluorescent probe for ONOO ‑ It exhibits good reactivity and is capable of specifically imaging endogenous ONOO in living cells or in vivo systems. ‑ Furthermore, it exhibits a large Stokes shift and an emission wavelength of 760 nm, significantly higher than other near-infrared fluorescent probes based on hemicyanine dye structures. In addition, this ONOO... ‑ The near-infrared emission characteristics of near-infrared fluorescent probes enable them to penetrate deeper into tissues and reduce background interference in bioimaging, which is significant for improving imaging sensitivity and accuracy. Based on these characteristics, ONOO... ‑ Near-infrared fluorescent probes can be used for the diagnosis and research of diseases such as atherosclerosis, neurodegenerative diseases, inflammation, and cancer.

According to the high-throughput drug screening method and system based on large-view-field imaging and machine learning, data acquisition of high-throughput organs or tumor balls is achieved through a large-view-field biological imaging system, the data acquisition process is accelerated, and the integrity of original image information is guaranteed; a two-stage image processing means that a lightweight target detection model is connected with a semantic segmentation model is adopted, so that the calculation parameter quantity is reduced, heterogeneity interference is removed, and the image information integrity is ensured while the accurate acquisition of the individual image contour of the organoid or the tumor ball is realized; for organoid image acquisition, a Z-axis scanning strategy is combined with a screening strategy based on IOU and a definition function, so that information acquisition integrity and subsequent organoid activity prediction precision are ensured; in combination with a machine learning model based on an ensemble learning principle for a regression task, the activity prediction of the bright field image of the organoid or tumor sphere is realized, and the influence of a traditional method on the activity of a culture is avoided.