179 results about "Biological imaging" patented technology

The invention discloses a super-resolution structured light reconstruction method based on a wavelet enhanced CNN-Transformer structure. The super-resolution structured light reconstruction method comprises the following steps of: firstly, carrying out wavelet enhancement on a CNN-Transformer structure; according to the method, image reconstruction is realized based on a WECT network adopting a coding-decoding architecture; the method comprises the following steps: firstly, extracting initial features through shallow convolution, performing two-stage down-sampling by using discrete wavelet transform, and respectively inputting results into a convolutional neural network and a Swin Transform module to perform parallel feature extraction and fusion; secondly, performing two-stage up-sampling through inverse discrete wavelet transform, and performing jump connection with shallow layer features; finally, pixel shuffling up-sampling operation is introduced, and residual jump connection between the input image and the output image is established; according to the method, high-resolution details can be efficiently recovered while the structure reduction precision is ensured, the image definition and contrast are enhanced, and the application value and practicability of the SIM image in scenes such as biological imaging and tissue analysis are improved.

The invention discloses an aza-BODIPY biomedical phototherapy agent taking oxajulolidine as a donor and a preparation method thereof, and relates to a medical phototherapy agent for treating brain glioma and a preparation method thereof, and the prepared compound is prepared by introducing an oxajulolidine fragment with electron donating property into a key position of an aza-BODIPY skeleton, so that the aza-BODIPY biomedical phototherapy agent is obtained. The donor-acceptor type molecular near-infrared phototherapy agent with a new structure is constructed, has good near-infrared absorption and emission performance and excellent stability, and shows a remarkable effect in the field of cancer photodynamic (PDT)-photothermal (PTT) synergistic treatment. The structural categories of the near-infrared aza-BODIPY biomolecules are enriched, and a new molecular platform is provided for expansion and application of the near-infrared aza-BODIPY biomolecules in the fields of biological imaging, tumor diagnosis and treatment, functional materials and the like.

According to the super-resolution polarized light acoustic imaging device and method, galvanometer scanning is achieved on the surface of a sample, excitation light is modulated in different linear polarization directions on each scanning point on the surface of the sample, and the intensity of photoacoustic signals generated by the sample under the excitation light in the different linear polarization directions is obtained through measurement; an initial photoacoustic image of a sample surface structure is constructed by utilizing photoacoustic signal intensities under exciting light in different linear polarization directions, and the initial photoacoustic image is subjected to phase-locked amplification and three-dimensional deconvolution processing, so that a super-resolution photoacoustic image in each linear polarization state is obtained. Compared with traditional polarized light acoustic imaging, the method has the advantages that the diffraction limit of traditional photoacoustic imaging can be overcome, super-resolution imaging of the surface structure of the sample can be achieved while unmarked imaging is achieved, and finer expression of the structure of the sample is achieved. The method has potential application prospects in the fields of biological imaging and biological tissue surface structure conformation detection.

The invention discloses a near-infrared fluorescent probe based on a xanthene-quinoline conjugated skeleton for detecting Cu < 2 + > as well as a preparation method and bioimaging application of the near-infrared fluorescent probe. The molecular formula of the fluorescent probe is C34H32N4O2. Based on the strategy of inhibiting the PET process, the large Stokes shift near-infrared fluorescent probe for specifically recognizing Cu < 2 + > is constructed, and spectrum experiments show that the probe has excellent recognition performance on Cu < 2 + >, the emission wavelength is 720 nm, and the Stokes shift reaches 170 nm; (2) the specificity is high, and Cu < 2 + > and other biological related metal ions can be effectively distinguished; (3) low detection limit (LOD = 17.6 nM); (4) quick response dynamics (lt; 30s) for a motor vehicle. More importantly, the probe XQ-NH2 is successfully applied to Cu < 2 + > imaging in living cells and living mice, it is proved that XQ-NH2 not only can provide a reliable technical means for Cu < 2 + > detection and imaging research in a complex biological system, but also shows that XQ-NH2 has important application potential in related fields.

The invention belongs to the technical field of fluorescent polymer materials, and particularly discloses bifuran polyamide with high quantum yield and capable of emitting yellow-green wavelength light as well as a preparation method and application of the bifuran polyamide. The method comprises the following steps: dissolving a bifuranoyl chloride monomer in an organic solvent to obtain an organic phase; adding an alkaline aqueous solution into the organic phase to form a phase interface, then adding a diamine monomer, and stirring to carry out an interfacial polymerization reaction; and after the reaction is finished, filtering, precipitating, washing and drying to obtain the bifuran polyamide. The bifuran polyamide is prepared by regulating and controlling the main chain structure and chain rigidity and flexibility of the polymer through a diamine monomer with a specific molecular structure, the emission wavelength of the amorphous bifuran polyamide can reach 540-552 nm under exciting light of 480 nm, red shift is performed to 565-568 nm after crystallization, long-wavelength yellow-green light emission, high-quantum-yield light emission and good biocompatibility are achieved, and the bifuran polyamide can be applied to the field of organic light emitting devices, such as organic light emitting devices, organic light emitting devices, organic light emitting devices, organic light emitting devices, organic light emitting devices, organic light emitting devices, organic light emitting devices and the like. The application of the bio-based polyamide in the field of biological imaging is expanded.

There is provided a bioimaging system for determining a spatial distribution of one or more physical properties within a biological structure, the system comprising: a data input configured to receive a plurality of measurements related to one or more physical fields; and a processing device configured to determine a spatial distribution of one or more physical properties within the biological structure using the plurality of measurements. The processing device forms a discretized full order model of the biological structure, describes a baseline spatial distribution of the biological structure using the model, and determines optimized values of coefficients describing the baseline spatial distribution based on the plurality of measurements. The processing device also forms a hybrid model comprising baseline spatial distribution coefficients and a set of deviation full-order model coefficients describing deviations relative to the baseline spatial distribution, determines optimized values for the baseline spatial distribution coefficients and the deviation full-order model coefficients of the hybrid model, and determining a spatial distribution of one or more physical properties within the biological structure using the optimized values of the coefficients.

The invention discloses a zebra fish imaging system, and belongs to the technical field of biological imaging detection, the zebra fish imaging system comprises a stirring module and an imaging module, the stirring module comprises a stirring mechanism, the stirring mechanism comprises a hollow stirring shaft, the upper end of the hollow stirring shaft is connected with a sample loading pipe, and the lower end of the stirring shaft is provided with a sample loading bin; the imaging module comprises a first platform, an imaging area is arranged on the first platform, a detection tube is arranged in the imaging area, the sample loading tube is connected in series with the detection tube, a rotary driving device is arranged in the imaging area along the axial direction of the detection tube, and a detection positioning module is arranged in the imaging area along the axial direction vertical to the detection tube. According to the scheme, no-dead-corner observation of the zebra fish is achieved, the imaging efficiency and imaging quality of the zebra fish are greatly improved, continuous detection is achieved, and a methodological tool is provided for medicine screening and development research of the zebra fish.

The invention discloses a method for preparing a one-dimensional organic metal halide single crystal through a solvent regulation and control method and application of the one-dimensional organic metal halide single crystal. According to the method, BBI-ZnCl2 and BBI-ZnCl4 single crystals are respectively prepared by taking bibenzimidazole and ZnCl2 as raw materials and adjusting the volume ratio of a methanol / water mixed solvent, the process is simple, the condition is mild, and complex equipment is not needed. After the obtained single crystal is compounded with polymethyl methacrylate, a material with excellent room-temperature phosphorescence performance is formed, and continuous afterglow emission can be generated under excitation of 365 nm ultraviolet light. By regulating and controlling the light-emitting characteristic difference of BBI-ZnCl2 and BBI-ZnCl4, the material can realize dynamic information hiding and display (such as pattern switching after ultraviolet irradiation / closing), and has outstanding advantages in the field of encryption and anti-counterfeiting. In addition, the material has the characteristics of cheap raw materials, good stability and no toxicity, and is also suitable for the fields of fire safety, biological imaging and the like. The preparation method has the innovativeness of the synthesis method and the practicability of the functional material, and has a wide industrialization prospect.

The present invention discloses a class of quinoacridine-5,9-diketone MR-TADF red light emitting materials and their preparation methods and applications, belonging to the technical field of organic photoelectric materials. The molecules of this type of material promote the minimization of bonding / antibonding of frontier molecular orbitals through the resonance effect generated by electron-deficient nitrogen / carbonyl and electron-rich oxygen / nitrogen. In addition, the rigid conjugated skeleton suppresses the structural relaxation of the molecular excited state and the vibration coupling between the excited state and the ground state, thereby obtaining a red light emitting material with a narrow half-width (<70nm) and high luminous efficiency; the introduction of a tert-butyl group on the periphery of the molecule can reduce the reorganization energy of the molecule; grafting different substituent units can regulate the stacking and film-forming properties of the molecule. The maximum emission peak of the obtained doped red light electroluminescent device is located at 601 nm, the half-width is 68 nm, and the corresponding maximum external quantum efficiency is 9.38%, which has a wide range of applications in the fields of organic electroluminescent displays and biological imaging.

The invention discloses a fluorescent pigment compound as well as a preparation method and application thereof. The chemical structural formula of the fluorescent pigment compound is shown in the specification. Wherein n is an integer from 2 to 6, and R1 and R2 are respectively and independently selected from C1-C3 alkyl groups. By introducing 4-tert-butylphenyl and tert-butylsilane ether chains, the compound has excellent solubility, film-forming property, high fluorescence quantum yield (gt, 95%) and light stability (retention rate gt, 90% after ultraviolet irradiation for 500 hours). The preparation method is simple, is suitable for a solution processing technology, and can be widely applied to the fields of OLEDs, light conversion adhesive films, fluorescence sensing, biological imaging and the like.

This invention relates to ONOO ‑ In the field of detection technology, specifically involving an ONOO ‑ Near-infrared fluorescent probe, its preparation method, and its application. This invention constructs a novel fluorescent probe specifically for detecting ONOO. ‑ The ONOO ‑ Near-infrared fluorescent probe for ONOO ‑ It exhibits good reactivity and is capable of specifically imaging endogenous ONOO in living cells or in vivo systems. ‑ Furthermore, it exhibits a large Stokes shift and an emission wavelength of 760 nm, significantly higher than other near-infrared fluorescent probes based on hemicyanine dye structures. In addition, this ONOO... ‑ The near-infrared emission characteristics of near-infrared fluorescent probes enable them to penetrate deeper into tissues and reduce background interference in bioimaging, which is significant for improving imaging sensitivity and accuracy. Based on these characteristics, ONOO... ‑ Near-infrared fluorescent probes can be used for the diagnosis and research of diseases such as atherosclerosis, neurodegenerative diseases, inflammation, and cancer.

According to the high-throughput drug screening method and system based on large-view-field imaging and machine learning, data acquisition of high-throughput organs or tumor balls is achieved through a large-view-field biological imaging system, the data acquisition process is accelerated, and the integrity of original image information is guaranteed; a two-stage image processing means that a lightweight target detection model is connected with a semantic segmentation model is adopted, so that the calculation parameter quantity is reduced, heterogeneity interference is removed, and the image information integrity is ensured while the accurate acquisition of the individual image contour of the organoid or the tumor ball is realized; for organoid image acquisition, a Z-axis scanning strategy is combined with a screening strategy based on IOU and a definition function, so that information acquisition integrity and subsequent organoid activity prediction precision are ensured; in combination with a machine learning model based on an ensemble learning principle for a regression task, the activity prediction of the bright field image of the organoid or tumor sphere is realized, and the influence of a traditional method on the activity of a culture is avoided.

This invention discloses a fluorescence-enhancing nanoparticle, its preparation method, and its applications, relating to the fields of organic fluorescent molecules and bioimaging technology. The nanoparticle comprises: NIR-II fluorescent molecules and an amphiphilic polymer encapsulating the NIR-II fluorescent molecules; the amphiphilic polymer has a hydrophilic segment and a rigid hydrophobic segment. The nanoparticle of this invention, based on "carrier microenvironment engineering," utilizes the rigid confinement and interfacial isolation provided by the amphiphilic polymer with the rigid hydrophobic segment for dual brightening, serving as a universal brightening platform applicable to various NIR-II fluorescent molecules. This nanoparticle can significantly improve aqueous phase quantum yield, enhance contrast and resolution in in vivo vascular imaging, improve deep tissue penetration, increase imaging sensitivity, and facilitate detection under low signal conditions. It also exhibits good stability and biocompatibility, supporting lesion imaging and intraoperative navigation applications.

The near-infrared window of fluorescent heptamethine cyanine dyes greatly facilitates biological imaging because there is deep penetration of the light and negligible background fluorescence. But dye instability, aggregation, and poor pharmacokinetics are current drawbacks that limit performance and the scope of possible applications. All these limitations are simultaneously overcome with a new molecular design strategy that produces a charge balanced and sterically shielded fluorochrome. The key design feature is a meso-Aryl group that simultaneously projects two shielding arms directly over each face of a linear heptamethine polyene. Cell and mouse imaging experiments compared a shielded heptamethine cyanine dye (and several peptide and antibody bioconjugates) to benchmark heptamethine dyes and found that the shielded systems possess an unsurpassed combination of photophysical, physiochemical and biodistribution properties that greatly enhance bioimaging performance.

The invention discloses a dioxetane chemiluminescent probe suitable for a biological orthogonal catalytic reaction as well as synthesis and application of the dioxetane chemiluminescent probe. A phenolic hydroxyl group active site of the probe is protected by an allyl bio-orthogonal cleavable group, and when a ruthenium metal complex catalyst is not added, the probe is in a silence state, and a chemiluminescence signal is weak; and in the presence of the ruthenium catalyst, the protecting group can be specifically catalytically cracked and removed, so that the probe is quickly activated and converted into a luminous'on 'state, and a strong chemiluminescence signal is generated. According to the invention, efficient combination of biological orthogonal cutting reaction and chemiluminescence signal output is realized, and the prepared biological orthogonal chemiluminescence probe has an excellent on-off ratio and is suitable for high-contrast biological imaging of living cell and living body levels.

This invention discloses a biomedical near-infrared luminescent material with a fluorite structure and its preparation method. Its chemical composition is given by [formula omitted], where 0 < x ≤ 0.1, preferably 0.001 ≤ x ≤ 0.05. This material belongs to a cubic fluorite structure, exhibiting not only excellent chemical stability but also superior biocompatibility due to its crystal structure characteristics. The luminescent material is prepared using a high-temperature solid-state method, with an excitation wavelength range covering 300-700 nm and multiple excitation peaks. It can be excited by blue, green, or red light, achieving far-red to near-infrared light emission in the wavelength range of 600-1100 nm. This material can be combined with visible light LED chips to fabricate near-infrared luminescent devices, possessing significant application value in fields such as bioimaging, tissue sensing, and non-invasive physiological detection. The preparation process of this invention is simple, the raw material cost is low, and it is suitable for large-scale production, demonstrating promising prospects for widespread application.

The invention relates to a wide-band excited near-infrared light-emitting metal halide with red light excitation response as well as preparation and application of the wide-band excited near-infrared light-emitting metal halide. The chemical composition formula of the metal halide is Rb < 2-x > CsxHf < 1-y > MoyCl6, and x is greater than or equal to 0 and less than or equal to 2; 0 < y < = 1. The emission spectrum of the near-infrared metal halide is 800-1400 nm, and the central wavelength of an emission peak is about 925 nm; an excitation peak covers a red light area, and the quantum dot has excellent wide-band excitation response capability and relatively high quantum yield. The preparation method is simple in process, large-scale preparation and technical popularization are easy to realize, and the preparation method is of great significance to development of efficient near-infrared fluorescent powder with wide-band excitation response. The near-infrared photoelectric device prepared from the metal halide shows potential application prospects in the fields of near-infrared night vision, biological imaging and the like.

The invention belongs to the technical field of organic luminescent materials, and relates to an organic luminescent material with visible light excitation and adjustable room temperature phosphorescence characteristics and a preparation method thereof. According to the method, an aromatic fused ring which does not contain heavy atoms and heteroatoms is taken as a doping object, anthraquinone and derivatives thereof are taken as a host matrix, doping is carried out through a solution volatilization method or a melting cooling method, and the organic luminescent material with visible light excitation and adjustable room temperature phosphorescence characteristics is obtained. Ground state energy transfer between a subject and an object is constructed, so that the material absorbs red and shifts to a visible light region, triplet emission of the subject is excited, an intersystem crossing process of the object is excited, and efficient room-temperature phosphorescence emission of the material under visible light and even white light is realized; the energy transfer intensity between a subject and an object is regulated and controlled by changing the length of a subject alkyl chain, effective adjustment of material phosphorescence from green to red is achieved, and the method has wide application prospects in the fields of information storage, data encryption and counterfeiting prevention, special printing technologies, programmable labels, biological imaging and the like.

The invention belongs to the technical field of hydrogen bond organic framework materials, and relates to a chiral luminescent ionic hydrogen bond organic framework material, the chemical formula is [(H2CBS) 4 (R / S-DPEN) 4. (EtOH) 2 (H2O) 18] n, n is a positive integer, H2CBS is 4, 4 '-bis (2-sulfostyryl) biphenyl, and R / S-DPEN is R / S-diphenylethylenediamine. The structural unit belongs to a triclinic system, the space group is P1, and the molecular formula is C177H222N8O50S8. The preparation method comprises the following steps: dissolving an H2CBS ligand and an R / S-DPEN ligand in a mixed solution of water and ethanol to obtain a reaction solution; and carrying out sealed heat preservation on the reaction liquid at 5-8 DEG C to obtain colorless transparent rod-like crystals, filtering and drying to obtain the chiral luminous ionic hydrogen bond organic framework material. The material has high light-emitting quantum yield and good structural stability and reversibility, and has important application value in the fields of organic light-emitting diodes (OLEDs), biological imaging, photoelectric devices and the like.

The application is suitable for the technical field of nanocluster preparation, and provides reversible control fluorescent gold nanoclusters and a preparation method thereof, which comprises the following steps: adding 4mM chloroauric acid solution 221µL into a CK solution with a concentration of 2mM 443µL after being fully dissolved, then adding 2M NaOH 14.7µL, adjusting the pH value of the solution to 13, immediately mixing, wrapping with tin paper, transferring to a shaking incubator for reaction for 10h, and obtaining H-AuNCs with bright red fluorescence. 60µM H-AuNCs are co-granted with HUVEC cells for 2h, and bright red fluorescence is formed in the cells, so that biological imaging can be realized. The powder obtained by drying the H-AuNCs is transferred into a dimethyl sulfoxide solution to obtain organic phase D-AuNCs with bright blue fluorescence. The application prepares CK-AuNCs with high fluorescence intensity, and can realize reversible adjustment of the fluorescence color.

The invention relates to the technical field of biological imaging, and discloses a macromolecular morphology restoration method based on microscopic imaging, which can achieve accurate restoration of macromolecular morphology more simply and conveniently. The method comprises the following steps: generating a grey-scale map according to atomic force microscopic imaging data, wherein the grey-scale map comprises a plurality of macromolecules; applying a migration model to the grey-scale map, performing image detection, extracting an image of macromolecular single particles, filtering defective particles, and retaining conventional particles; wherein the migration model is obtained based on a segmentation model by training data sets in different view ranges to perform multiple rounds of learning; and aligning the images of the conventional particles through an image registration algorithm and averaging to obtain a macromolecule morphology restored image.

The invention discloses a quick response type near-infrared fluorescent probe for detecting Hg < 2 + >, a preparation method of the quick response type near-infrared fluorescent probe and application of the quick response type near-infrared fluorescent probe in actual water samples and biological imaging. The molecular formula of the fluorescent probe is C32H28N2O2S2. On the basis of a strategy of introducing phenothiazine heterocycle to expand xanthene pi conjugation, a large Stokes shift near infrared fluorescent probe for specifically recognizing Hg < 2 + > is constructed. An optical experiment shows that the emission wavelength of the probe for detecting Hg < 2 + > is 780nm, the Stokes shift reaches 166nm, and the probe has the advantages of high sensitivity (the detection limit is 13.4 nM), specificity, quick response (within 1s) and the like when being used for identifying Hg < 2 + >. Due to the excellent photophysical property, the fluorescent probe not only can be used for quantitatively detecting Hg < 2 + > in an environmental water sample, but also can be successfully applied to fluorescence imaging detection of Hg < 2 + > in cells and living bodies.

The invention belongs to the technical field of organic functional materials, and particularly relates to an N-substituted phenothiazine derivative and a preparation method and application thereof.The N-substituted phenothiazine derivative comprises at least one of a unilateral compound and a bilateral compound, the structural formula of the unilateral compound is shown in the specification, and the structural formula of the bilateral compound is shown in the specification. According to the N-substituted phenothiazine derivative disclosed by the invention, the conjugation degree of a molecular skeleton is adjusted and an electron withdrawing group F atom is introduced to a peripheral benzene ring of a unit A to regulate and control overall electron distribution, so that the synthesized N-substituted phenothiazine derivative has unique photophysical properties and high selectivity, high sensitivity and high responsiveness to hypochlorite ions; the method can be widely applied to the fields of chemical sensing, environment detection, biological imaging and the like.

The invention is applicable to the technical field of chemical materials, and provides a method for regulating and controlling luminescence of Au nanoclusters by solvent-induced ligand isomerization, which comprises the following steps: firstly, synthesizing the gold nanoclusters with different surface isomer proportions by regulating the pH value of a reaction system; au (I)-thioketone and Au (I)-mercaptan isomer are induced to be reversibly converted by utilizing the interaction of solvent molecules and a cluster surface ligand structure, and proton coupling electron transfer kinetics is cooperatively regulated, so that the precise regulation of the luminescence property of the gold nanocluster is realized. According to the present invention, the wide band and the continuous reversible adjustment of the light-emitting wavelength in the range of 495-800 nm can be achieved under the mild condition, the light-emitting quantum yield can be significantly improved, and the method has advantages of simple operation, clear mechanism, greenness and universality, and has application prospects in the fields of biological imaging, optical sensing, light-emitting devices and the like.

The invention discloses a polypeptide nanoring material and a preparation method thereof, and belongs to the technical field of high polymer materials. The preparation method comprises the following steps: co-assembling poly (gamma-benzyl ester-L-glutamic acid) (PBLG) and achiral triphenylamine derivatives {N, N-bis (2-methylphenyl) aniline (MTPA), 4-(5-aldehyde-2, 2 ': 5', 2 ''-terthienyl)-N, N-bis (2-methylphenyl) aniline (MTTH) or 4-[5-(2, 2-dicyanovinyl) thiophene-2-yl]-N, N-bis (2-methylphenyl) aniline (MTTV)} to obtain the poly (gamma-benzyl ester-L-glutamic acid)-N, N-bis (2-methylphenyl) aniline derivative. According to the present invention, the emission of multi-color circularly polarized light (CPL) (near ultraviolet to deep red light, 370-660 nm) is achieved, and the maximum luminescence asymmetry factor (glum) is up to 1.1 * 10 <-2 >, and is improved by more than 30% compared with the prior art. Meanwhile, the generation inhibition rate of reactive oxygen species (ROS) is increased to 98% or above through a compact nanostructure of the material, the problem of phototoxicity of traditional triphenylamine derivatives is solved, and the material is suitable for high-resolution biological imaging and targeted diagnosis and treatment.

The present invention discloses a broadband near-infrared luminescent material, a preparation method thereof, and a near-infrared LED light source, belonging to the technical field of near-infrared luminescent materials. The broadband near-infrared luminescent material provided by the present invention has a structure shown in formula (I), Ca 4‑x Zn x HfGe3O 12 :yCr 3+ Formula (I), wherein 0 < x ≤ 1, 0.01 ≤ y ≤ 0.10. By substituting Zn 2+ for Ca 2+ , the problem of lattice mismatch is solved, and the luminous intensity and thermal stability are simultaneously improved. The method is simple and "brings three benefits at one stroke"; it can absorb blue light in the range of 400-550 nm and obtain a broadband near-infrared emission with an emission peak range of 650-1200 nm under the excitation of 470 nm; it has a long emission wavelength (>800 nm), high efficiency (high IQE, EQE and AE values), and high thermal stability. The integrated intensity can still maintain more than 80% of room temperature at a high temperature of 400 K, and has great application potential in night vision, biological imaging, rapid recognition and other aspects.

The application discloses a multi-point focusing photoacoustic endoscopic imaging catheter and relates to the technical field of biological imaging. The multi-point focusing photoacoustic endoscopic imaging catheter comprises a shell, a torque coil, an ultrasonic transducer, an optical fiber, a focusing lens, at least one light splitting prism and a mirror which are coaxially arranged along an optical path in sequence, the focusing lens, the at least one light splitting prism, the ultrasonic transducer and the mirror are arranged in the interior of the shell, one end of the optical fiber is fixed in the shell, and a gap is formed between the optical fiber and the focusing lens; each light splitting prism is used for splitting an incident light beam into a reflected first excitation light beam and a transmitted second excitation light beam, the first excitation light beam and the light beam emitted after being reflected by the mirror both converge right above the ultrasonic transducer, and the focal points of the first excitation light beam and the light beam emitted after being reflected by the mirror do not overlap. The application is used for obtaining a larger imaging range and acquiring a complete high-resolution endoscopic image.