98 results about "Biological imaging" patented technology

The invention discloses an aza-BODIPY biomedical phototherapy agent taking oxajulolidine as a donor and a preparation method thereof, and relates to a medical phototherapy agent for treating brain glioma and a preparation method thereof, and the prepared compound is prepared by introducing an oxajulolidine fragment with electron donating property into a key position of an aza-BODIPY skeleton, so that the aza-BODIPY biomedical phototherapy agent is obtained. The donor-acceptor type molecular near-infrared phototherapy agent with a new structure is constructed, has good near-infrared absorption and emission performance and excellent stability, and shows a remarkable effect in the field of cancer photodynamic (PDT)-photothermal (PTT) synergistic treatment. The structural categories of the near-infrared aza-BODIPY biomolecules are enriched, and a new molecular platform is provided for expansion and application of the near-infrared aza-BODIPY biomolecules in the fields of biological imaging, tumor diagnosis and treatment, functional materials and the like.

The invention belongs to the technical field of fluorescent polymer materials, and particularly discloses bifuran polyamide with high quantum yield and capable of emitting yellow-green wavelength light as well as a preparation method and application of the bifuran polyamide. The method comprises the following steps: dissolving a bifuranoyl chloride monomer in an organic solvent to obtain an organic phase; adding an alkaline aqueous solution into the organic phase to form a phase interface, then adding a diamine monomer, and stirring to carry out an interfacial polymerization reaction; and after the reaction is finished, filtering, precipitating, washing and drying to obtain the bifuran polyamide. The bifuran polyamide is prepared by regulating and controlling the main chain structure and chain rigidity and flexibility of the polymer through a diamine monomer with a specific molecular structure, the emission wavelength of the amorphous bifuran polyamide can reach 540-552 nm under exciting light of 480 nm, red shift is performed to 565-568 nm after crystallization, long-wavelength yellow-green light emission, high-quantum-yield light emission and good biocompatibility are achieved, and the bifuran polyamide can be applied to the field of organic light emitting devices, such as organic light emitting devices, organic light emitting devices, organic light emitting devices, organic light emitting devices, organic light emitting devices, organic light emitting devices, organic light emitting devices and the like. The application of the bio-based polyamide in the field of biological imaging is expanded.

There is provided a bioimaging system for determining a spatial distribution of one or more physical properties within a biological structure, the system comprising: a data input configured to receive a plurality of measurements related to one or more physical fields; and a processing device configured to determine a spatial distribution of one or more physical properties within the biological structure using the plurality of measurements. The processing device forms a discretized full order model of the biological structure, describes a baseline spatial distribution of the biological structure using the model, and determines optimized values of coefficients describing the baseline spatial distribution based on the plurality of measurements. The processing device also forms a hybrid model comprising baseline spatial distribution coefficients and a set of deviation full-order model coefficients describing deviations relative to the baseline spatial distribution, determines optimized values for the baseline spatial distribution coefficients and the deviation full-order model coefficients of the hybrid model, and determining a spatial distribution of one or more physical properties within the biological structure using the optimized values of the coefficients.

The invention discloses a fluorescent pigment compound as well as a preparation method and application thereof. The chemical structural formula of the fluorescent pigment compound is shown in the specification. Wherein n is an integer from 2 to 6, and R1 and R2 are respectively and independently selected from C1-C3 alkyl groups. By introducing 4-tert-butylphenyl and tert-butylsilane ether chains, the compound has excellent solubility, film-forming property, high fluorescence quantum yield (gt, 95%) and light stability (retention rate gt, 90% after ultraviolet irradiation for 500 hours). The preparation method is simple, is suitable for a solution processing technology, and can be widely applied to the fields of OLEDs, light conversion adhesive films, fluorescence sensing, biological imaging and the like.

According to the high-throughput drug screening method and system based on large-view-field imaging and machine learning, data acquisition of high-throughput organs or tumor balls is achieved through a large-view-field biological imaging system, the data acquisition process is accelerated, and the integrity of original image information is guaranteed; a two-stage image processing means that a lightweight target detection model is connected with a semantic segmentation model is adopted, so that the calculation parameter quantity is reduced, heterogeneity interference is removed, and the image information integrity is ensured while the accurate acquisition of the individual image contour of the organoid or the tumor ball is realized; for organoid image acquisition, a Z-axis scanning strategy is combined with a screening strategy based on IOU and a definition function, so that information acquisition integrity and subsequent organoid activity prediction precision are ensured; in combination with a machine learning model based on an ensemble learning principle for a regression task, the activity prediction of the bright field image of the organoid or tumor sphere is realized, and the influence of a traditional method on the activity of a culture is avoided.

This invention discloses a fluorescence-enhancing nanoparticle, its preparation method, and its applications, relating to the fields of organic fluorescent molecules and bioimaging technology. The nanoparticle comprises: NIR-II fluorescent molecules and an amphiphilic polymer encapsulating the NIR-II fluorescent molecules; the amphiphilic polymer has a hydrophilic segment and a rigid hydrophobic segment. The nanoparticle of this invention, based on "carrier microenvironment engineering," utilizes the rigid confinement and interfacial isolation provided by the amphiphilic polymer with the rigid hydrophobic segment for dual brightening, serving as a universal brightening platform applicable to various NIR-II fluorescent molecules. This nanoparticle can significantly improve aqueous phase quantum yield, enhance contrast and resolution in in vivo vascular imaging, improve deep tissue penetration, increase imaging sensitivity, and facilitate detection under low signal conditions. It also exhibits good stability and biocompatibility, supporting lesion imaging and intraoperative navigation applications.

The invention discloses a dioxetane chemiluminescent probe suitable for a biological orthogonal catalytic reaction as well as synthesis and application of the dioxetane chemiluminescent probe. A phenolic hydroxyl group active site of the probe is protected by an allyl bio-orthogonal cleavable group, and when a ruthenium metal complex catalyst is not added, the probe is in a silence state, and a chemiluminescence signal is weak; and in the presence of the ruthenium catalyst, the protecting group can be specifically catalytically cracked and removed, so that the probe is quickly activated and converted into a luminous'on 'state, and a strong chemiluminescence signal is generated. According to the invention, efficient combination of biological orthogonal cutting reaction and chemiluminescence signal output is realized, and the prepared biological orthogonal chemiluminescence probe has an excellent on-off ratio and is suitable for high-contrast biological imaging of living cell and living body levels.

This invention discloses a biomedical near-infrared luminescent material with a fluorite structure and its preparation method. Its chemical composition is given by [formula omitted], where 0 < x ≤ 0.1, preferably 0.001 ≤ x ≤ 0.05. This material belongs to a cubic fluorite structure, exhibiting not only excellent chemical stability but also superior biocompatibility due to its crystal structure characteristics. The luminescent material is prepared using a high-temperature solid-state method, with an excitation wavelength range covering 300-700 nm and multiple excitation peaks. It can be excited by blue, green, or red light, achieving far-red to near-infrared light emission in the wavelength range of 600-1100 nm. This material can be combined with visible light LED chips to fabricate near-infrared luminescent devices, possessing significant application value in fields such as bioimaging, tissue sensing, and non-invasive physiological detection. The preparation process of this invention is simple, the raw material cost is low, and it is suitable for large-scale production, demonstrating promising prospects for widespread application.

The invention belongs to the technical field of organic luminescent materials, and relates to an organic luminescent material with visible light excitation and adjustable room temperature phosphorescence characteristics and a preparation method thereof. According to the method, an aromatic fused ring which does not contain heavy atoms and heteroatoms is taken as a doping object, anthraquinone and derivatives thereof are taken as a host matrix, doping is carried out through a solution volatilization method or a melting cooling method, and the organic luminescent material with visible light excitation and adjustable room temperature phosphorescence characteristics is obtained. Ground state energy transfer between a subject and an object is constructed, so that the material absorbs red and shifts to a visible light region, triplet emission of the subject is excited, an intersystem crossing process of the object is excited, and efficient room-temperature phosphorescence emission of the material under visible light and even white light is realized; the energy transfer intensity between a subject and an object is regulated and controlled by changing the length of a subject alkyl chain, effective adjustment of material phosphorescence from green to red is achieved, and the method has wide application prospects in the fields of information storage, data encryption and counterfeiting prevention, special printing technologies, programmable labels, biological imaging and the like.

The invention relates to the technical field of biological imaging, and discloses a macromolecular morphology restoration method based on microscopic imaging, which can achieve accurate restoration of macromolecular morphology more simply and conveniently. The method comprises the following steps: generating a grey-scale map according to atomic force microscopic imaging data, wherein the grey-scale map comprises a plurality of macromolecules; applying a migration model to the grey-scale map, performing image detection, extracting an image of macromolecular single particles, filtering defective particles, and retaining conventional particles; wherein the migration model is obtained based on a segmentation model by training data sets in different view ranges to perform multiple rounds of learning; and aligning the images of the conventional particles through an image registration algorithm and averaging to obtain a macromolecule morphology restored image.

The invention is applicable to the technical field of chemical materials, and provides a method for regulating and controlling luminescence of Au nanoclusters by solvent-induced ligand isomerization, which comprises the following steps: firstly, synthesizing the gold nanoclusters with different surface isomer proportions by regulating the pH value of a reaction system; au (I)-thioketone and Au (I)-mercaptan isomer are induced to be reversibly converted by utilizing the interaction of solvent molecules and a cluster surface ligand structure, and proton coupling electron transfer kinetics is cooperatively regulated, so that the precise regulation of the luminescence property of the gold nanocluster is realized. According to the present invention, the wide band and the continuous reversible adjustment of the light-emitting wavelength in the range of 495-800 nm can be achieved under the mild condition, the light-emitting quantum yield can be significantly improved, and the method has advantages of simple operation, clear mechanism, greenness and universality, and has application prospects in the fields of biological imaging, optical sensing, light-emitting devices and the like.

The application belongs to the technical field of organic luminescent materials, and discloses a near-infrared organic scintillator material and a preparation method thereof. Based on the phosphorescence resonance energy transfer mechanism, the application uses an organic phosphorescence scintillator o-ITC crystal as an energy donor, and a near-infrared fluorescent luminophore RB or BBT-TPA as an energy acceptor. The near-infrared fluorescent luminophore material is dissolved in an organic solvent, and then the organic phosphorescence scintillator is added. The solvent is removed under specific conditions using a vacuum rotary evaporator, and finally the near-infrared organic scintillator material RB@o-ITC and BBT-TPA@o-ITC is obtained. The material effectively prolongs the luminescence wavelength of the organic scintillator. The maximum radiation luminescence wavelength of the BBT-TPA@o-ITC material reaches 920 nm, and the material has a wide application prospect in the fields of X-ray imaging, organic light-emitting diodes, biological imaging, advanced encryption and anti-counterfeiting, information storage and the like.

Systems for gradient surface electromagnetic wave generation and related methods are described. In some implementations, a first horn antenna may be configured to disrupt momentum conservation parallel to an interface between water and another material, enabling the generation of gradient surface electromagnetic waves. A second horn antenna, positioned in proximity to the first, may be adapted to generate anti-resonances in response to the gradient surface electromagnetic waves initiated by the first horn antenna. The presence of an object, which may be composed of metal or dielectric materials, near the interface may alter the anti-resonances generated by the horn antennas. This alteration in anti-resonances may indicate the presence of the object, thereby facilitating the detection of metallic and dielectric objects in close proximity to the water surface. This system and method may enhance biosensing and bioimaging applications by supplementing traditional x-ray and ultrasound techniques with gradient surface electromagnetic wave-based detection.

This invention relates to the field of fluorescent materials technology, disclosing near-infrared I-region photosensitizers, their preparation methods, and their applications in bioimaging. The near-infrared I-region photosensitizers include TPE-AT-DPTQ, TPE-EDOT-DPTQ, and TPE-HEXOT-DPTQ, possessing a D-π-A-π-D molecular structure. They utilize tetraphenylethylene as the electron donor, sterically hindered π-bridges (3-hexylthiophene, 3,4-ethylenedioxythiophene, and 3,4-dihexyloxythiophene) as bridging units, and DPTQ as the electron acceptor. By introducing long alkyl side chains, these near-infrared I-region photosensitizers significantly increase the intermolecular distance in the aggregated state, effectively preventing excessively dense molecular packing and further enhancing the AIE properties and fluorescence brightness of the molecules.

The invention discloses a cancer cell classification imaging system and application thereof. The cancer cell classification imaging system comprises a double-stranded DNA (deoxyribonucleic acid) structure S1 / S2 containing S1 and S2 single chains, and a target miRNA (micro Ribonucleic Acid)-started hybridization chain reaction system, the S2 single strand comprises a specific hybridization structural domain t1 and a specific hybridization structural domain t2; the specific hybridization structural domain t1 is complementary with the target miRNA; the expression level A of the target miRNA in cancer cells is higher than the expression level B of the target miRNA in normal cells; the expression level C of the non-target miRNA in cancer cells is lower than the expression level D of the non-target miRNA in normal cells. According to the method, the number of free target miRNA in normal cells is greatly reduced through a DNA molecular subtraction method; and the free target miRNA in the cancer cells is still maintained at a proper level to trigger the hybridization chain reaction, so that the problem of insufficient signal specificity of biological imaging of the cancer cells is solved.

This application discloses a nitric oxide-activated near-infrared fluorescent-photoacoustic compound, its preparation method, and its applications. The fluorescent-photoacoustic compound has the structure shown below, where R includes groups containing oxanthracene or indole structures, etc. Compared to existing technologies, the fluorescent-photoacoustic compound provided in this application has a longer excitation / emission wavelength, exhibiting not only a highly specific response to nitric oxide but also deeper tissue penetration, effectively avoiding interference from biofluorescence during bioimaging. It can be used for near-infrared fluorescence imaging and photoacoustic imaging, enabling real-time monitoring of changes in nitric oxide concentration at the in vivo level.

This invention relates to the field of biomedical imaging data processing technology, and discloses an AgInS quantum dot bioimaging image enhancement and recognition system and method. The system includes an excitation light source, a gated camera, a timing controller, and a data processing unit. The method includes: receiving image frames under different delay time windows to construct a three-dimensional spatiotemporal data matrix; orthogonalizing pre-calibrated long and short lifetime prior vectors to generate an initial target basis, and calculating the inner product with pixel temporal vectors to generate an initial target weight matrix; segmenting and eroding the matrix to extract a high-confidence background mask, and inversely mapping and averaging to generate a dynamic background basis; re-orthogonalizing and calculating the inner product using the dynamic background basis to obtain the final target weight matrix; numerically mapping the matrix and performing connected component analysis to output the results. This invention achieves real-time adaptive updating of background reference features, improving target contrast and target recognition accuracy in complex living environments.

The invention discloses a side chain substituted triphenylmethyl free radical with light-operated dual-emission characteristics as well as a preparation method and application thereof, and relates to the technical field of organic light-emitting materials. The meta-position of a triphenylmethyl free radical is used as a modification part, aromatic substituents with different characteristics are introduced through a Suzuki reaction to regulate and control the high-energy-state electron energy level, so that the material has light-operated dual-emission performance, the variety of light-emitting free radicals can be expanded, the application of the material in more fields is further realized, and the application range of the material is widened. The compounds can be used in the fields of optical sensing, electroluminescent devices, biological imaging and the like. The problem that in the prior art, triphenylmethyl free radicals which are stable in side chain substitution, light-emitting and have light-operated dual-emission characteristics are lacked is solved.

The invention discloses a method for repairing a probe for a biological test of a terahertz near-field system, and belongs to the technical field of terahertz near-field imaging. According to the method, aiming at the problem that the imaging quality is reduced due to the fact that a probe is polluted by water or water-containing tissues in the biological sample imaging process, the pollution condition is observed through an optical microscope, and two steps of adsorbate separation and adsorbate removal are sequentially carried out. Firstly, a small amount of neutral or weakly acidic solution is dropwise added near a needle tip for dissolution, the solution diffusion range is controlled, and contact with a substrate is avoided; after the adsorbate is separated, a large amount of solution is dropwise added for washing, suction paper is used for sucking dry from the side, and the operation is repeated to ensure that pollutants are completely removed. According to the method, mechanical external force is not needed, operation is safe, simple and reliable, the probe can be effectively repaired, the service life of the probe can be effectively prolonged, and application of a terahertz near-field system in biological imaging is promoted.

The invention discloses a preparation method and application of a transition metal ion doped CdSe / ZnS core-shell quantum dot, according to the method, transition metal ions are controllably introduced into a host CdSe / ZnS material forbidden band as a magazine energy level so as to create a new electron energy level, and meanwhile, the number of defect energy levels of the CdSe core quantum dot is remarkably reduced; and a light-emitting mechanism is successfully converted from band-edge exciton recombination to non-radiative transition recombination. And the dependence of the luminescence performance on the physical size of the quantum dot is eliminated. Due to the characteristic, the material has the excellent temperature response characteristic: the material has wide-spectrum and tunable luminescence, huge Stokes shift to eliminate self-absorption, excellent light stability and long fluorescence lifetime, and has the potential of low toxicity. Due to the advantages, the material has a huge application prospect in the fields of biological imaging, fluorescence detection, solid-state illumination and the like.

The invention discloses an application of a dibenzo quinoxaline compound in an organic phosphorescent material. The dibenzo quinoxaline compound has a structural formula as shown in a formula (I), wherein X1 and X2 are respectively and independently selected from H or halogen. The dibenzo quinoxaline compound provided by the invention can show efficient and stable room-temperature phosphorescence emission under various excitation conditions of ultraviolet light, visible light, X-rays and the like. And the organic room-temperature phosphorescent material prepared from the dibenzo quinoxaline compound has excellent thermal stability and shows a long-wave-band phosphorescent emission characteristic. The material can be used for high-precision biological imaging, and can be applied to X-ray imaging as an organic scintillator.

This invention discloses a near-infrared fluorescent silicon dot, its preparation method, and its application in antibacterial and tumor treatment. The silicon dot is generated via a one-step hydrothermal method through a high-temperature condensation reaction between the carboxyl group of a 4-carboxyporphyrin dye and the amino group of a silane coupling agent. The silicon dot synthesized in this invention exhibits excellent water dispersibility, high near-infrared fluorescence intensity and pH stability, uniform morphology, good photostability, good biocompatibility, and high reactive oxygen species (ROS) yield. Furthermore, the preparation method is simple and convenient, uses widely available raw materials, and is low-cost, making it suitable for industrial production. Based on these excellent properties, it can be used to prepare fluorescent probes as fluorescent markers for cell and biological imaging. Its excellent singlet oxygen generation performance can be used for photodynamic therapy against bacteria and tumors. In addition, the silicon dot also possesses peroxidase properties, catalyzing the generation of hydroxyl radicals from hydrogen peroxide, thus enabling its use in chemokinetic therapy.

This invention discloses a class of afterglow molecules, their preparation method, and applications, belonging to the field of biomedical optical imaging materials. By performing a Knoevenagel reaction between compound 2 (4-substituted-2,6-dicarboxyphenol) and N-substituted-2-methylbenzothiazolium bromide, inactive self-sustaining afterglow molecules QCAs are obtained. QCAs integrate reactive oxygen species generation, high-energy intermediate formation, and luminescence functions, possessing advantages such as simplified structure, low energy dissipation, and high reliability. Furthermore, an activatable self-sustaining afterglow molecule, P-QCA5, is also proposed. P-QCA5, modified with a phenylboronic acid group, can specifically respond to ONOO. ‑ It also activates the afterglow signal; leveraging its inherent low background and high signal-to-noise ratio, it successfully achieved precise imaging of mouse tumor models and Parkinson's disease (PD) models. Compared to traditional fluorescence imaging, which requires real-time excitation, afterglow imaging does not require real-time external light excitation, thus avoiding interference from tissue autofluorescence. It has a higher signal-to-noise ratio and deeper tissue detection depth, making it a promising bioimaging optical probe.

The application relates to a kind of 2H-chromene phenyl ketone derivatives of double halogen substitution and preparation method and application thereof, belong to the technical field of organic room temperature phosphor material technology.The preparation method of derivative of the application comprises the following steps: (1) under inert gas protection, with halogenated salicylaldehyde and 1-(3-bromine prop-1-alkyne-1-yl) halogenated benzene as raw material, potassium carbonate as base, acetonitrile as solvent, reflux reaction is carried out, the reaction solution is extracted, dried, column chromatography separation, and intermediate 1 is obtained; (2) under inert gas protection, intermediate 1 is dissolved in dichloromethane, boron trifluoride ether is added, and stirring reaction is carried out, and the reaction solution is extracted, dried, column chromatography separation, and the application is obtained.The synthesis route of derivative of the application is simple, high in yield, easy to purify, and the prepared room temperature phosphor material can be widely applied in the field of organic light emitting diode, information encryption, biological imaging and optical sensing.

The invention belongs to the technical field of organic photoelectric functional materials, and particularly relates to a luminescent material based on a conjugated modified dithienyl anthracene-phenanthroimidazole skeleton and a preparation method and application thereof. According to the invention, substituent groups (phenyl, naphthyl, anthryl, pyrenyl and triphenylamine) with different conjugation degrees are selectively introduced, and the system regulates the steric hindrance and electronic structure of molecules, so that collaborative optimization of mechanical stimuli response and aggregation-induced emission is successfully realized; the molecular engineering strategy can accurately control the force-induced discoloration behavior (blue-green-yellow reversible transformation) and AIE characteristics of the material, and keeps excellent biocompatibility at the same time. The method disclosed by the invention not only discloses a regulation and control rule of substituent conjugation degree and spatial configuration on material performance, but also provides a designable molecular platform for developing a novel intelligent luminescent material, and has important application value in the fields of anti-counterfeiting encryption, biological imaging, flexible photoelectricity and the like.

The invention discloses a near-infrared fluorescent probe with an aggregation-induced emission enhancement effect. The near-infrared fluorescent probe is used for dual detection of hydrogen sulfide and hydrazine. The fluorescent probe is synthesized by carrying out Knoevenagel condensation reaction on 4-hydroxy-3-(1, 4, 5-triphenyl-1H-imidazolyl) benzaldehyde (I) and 2-(2-methyl-4H-chromene-4-subunit) malononitrile (II) under the catalytic action of piperidine, and the chemical structural formula of the fluorescent probe is as shown in the formula (III). The fluorescent probe shows high selectivity and high sensitivity to both hydrogen sulfide and hydrazine, the detection limits are as low as 50.1 nM and 89.2 nM respectively, and the fluorescent probe can be used for various scenes such as food spoilage monitoring and environmental water sample detection, can be used for biological imaging, and shows important application potential in the aspect of detection of hydrazine and hydrogen sulfide in organisms.

A core-shell nanoparticle having a cleaned nitrogen-vacancy nanodiamond (NVND) core surrounded by an upconversion nanoparticle (UCNP) shell, wherein the cleaned nitrogen-vacancy nanodiamond (NVND) core comprises a cleaned nitrogen-vacancy nanodiamond, wherein the UCNP shell comprises an upconversion nanoparticle (UCNP), wherein the UCNP comprises lithium yttrium fluoride (LiYF4) doped with a lanthanide ion combination, wherein the lanthanide ion combination comprises a M1 ion and a M2 ion, wherein M1 is ytterbium (Yb) or neodymium (Nd) and M2 is erbium (Er), thulium (Tm) or holmium (Ho), and wherein the core-shell nanoparticle emits a red-emission upon a near-infrared (NIR) excitation is disclosed. Integration of cleaned NVNDs with UNCPs for enhancing optical manipulation and enabling efficient energy transfer for applications in biological imaging, quantum sensing and laser cooling is disclosed.

The invention provides a sisal hemp residue derived red light carbon dot photosensitizer as well as a preparation method and application thereof, and belongs to the technical field of nano functional materials and biomass resource utilization. The method comprises the following steps: dispersing the sisal residue in water, adding urea, stirring at room temperature, and carrying out hydrothermal reaction; and after the reaction is completed, filtering, dialyzing and drying a product in sequence to obtain the sisal hemp residue derived red light carbon dot photosensitizer. The method is wide in raw material source, low in cost, simple in process, green and environment-friendly. The particle size of the prepared red light carbon dot photosensitizer is 2-6 nm, the red light carbon dot photosensitizer has a remarkable red light emission peak at the position of 660-680 nm, especially can efficiently generate active oxygen under excitation of light with the wavelength of 660 nm, has a good application prospect in the fields of photodynamic therapy, photocatalytic disinfection, biological imaging and the like, and provides a new way for developing a novel photosensitizer which is low in cost, efficient and environmentally friendly.