271 results about "Fluorescent quenching" patented technology

The invention discloses a near-infrared two-region activatable probe as well as a preparation method and application thereof. The near-infrared second-region activatable probe comprises a near-infrared second-region fluorescent light-emitting unit and an analyte specific response unit; the near-infrared second-region light-emitting unit comprises a hemicyanine fluorophore structure; when the analyte specific response unit is chemically coupled with the near-infrared second-region light-emitting unit, the intramolecular charge transfer process of the light-emitting unit can be inhibited, and fluorescence quenching is caused; the analyte specific response unit is separated from the light-emitting unit after being subjected to specific reaction with an analyte, so that fluorescence is activated. The near-infrared two-region hemicyanine fluorophore selected by the near-infrared two-region activatable probe has higher fluorescence quantum efficiency, and the near-infrared two-region probe which can be activated by a specific analyte and has a high on-off ratio can be constructed by introducing an analyte specific response unit, so that high-temporal-spatial-resolution fluorescence detection of deep tissues can be realized; the method has a wide application prospect in the field of biomedical detection.

The invention relates to the technical field of fluorescent hydrogel, in particular to high-strength and high-stability fluorescent hydrogel as well as a preparation method and application thereof, the fluorescent hydrogel is formed by physical crosslinking of a hydrogel matrix, a rare earth fluorescent complex and a stabilizer, the hydrogel matrix is of a double-network structure formed by physical crosslinking of polyvinyl alcohol and carboxymethyl cellulose; the rare earth fluorescent complex is formed by connecting rare earth metal ions and an organic antenna material through coordination; the stabilizer is trisodium citrate. Compared with other fluorescent hydrogel, the fluorescent hydrogel with high strength and high stability provided by the invention has the advantages that the phenomena of fluorescence decay and fluorescence quenching caused by metal ions and saturated salt solution are avoided, and the fluorescence is kept stable in a wide pH range. Because the fluorescence characteristic is not influenced by environmental corrosion factors, the information can be stably displayed for a long time, and the method can be used for information encryption and anti-counterfeiting in an extreme environment.

The invention discloses a sensing material for detecting atmospheric carboxylic acid, a preparation method, a detection method and application, belongs to the technical field of environmental functional materials, and solves the problems that an existing method is complex in operation and cannot realize real-time online monitoring, the sensing material for detecting atmospheric carboxylic acid is an MOF-802-EDTA-Pt composite film, and the preparation method comprises the following steps: taking 1, 3, 4, 5-tetramethyl-1, 3, 5-tetramethyl-1, 3, 5-tetramethyl-1, 3, 5-tetramethyl-1, 3, 5-tetramethyl-1, 3, 5- The preparation method comprises the following steps: synthesizing an MOF-802 material through a hydrothermal reaction by taking 1, 3, 5-benzene tricarbonic acid (BTC) and aluminum nitrate as raw materials and ethylene diamine tetraacetic acid (Na-EDTA) as a ligand; the preparation method comprises the following steps: dispersing MOF-802 in DMF to form a coating solution, and forming a film on a glass substrate through a pulling method; and then loading platinum nanoparticles, and carrying out solvothermal reaction and vacuum drying to obtain the composite sensing film. The MOF-802-EDTA-Pt composite film has a porous structure and fluorescence characteristics, and detection is realized through a fluorescence quenching effect of carboxylic acid molecules and an MOF skeleton. The method disclosed by the invention is controllable in process and high in material stability, still keeps excellent selectivity under the coexistence condition of interferents such as CO2 and NO2, and is suitable for real-time monitoring of carboxylic acid pollutants in the atmospheric environment.

The present disclosure relates to methods for determining concentration of a lysine analog antifibrinolytic agent in a sample. Also provided are kits comprising (a) a composition comprising a fluorescently labeled inactive plasminogen variant and a fluorescence quencher labeled fibrin-degradation product (FDP) and (b) a lysine analog antifibrinolytic agent standard and methods of use thereof.

The invention discloses a preparation method of photoresponse COFs coated CeO2 nano-enzyme, and relates to the field of nano-materials. According to the invention, FePc, TAPP and 2, 5-dihydroxy-1, 4-phthalaldehyde are used for creating a core-shell material CeO2 (at) FePC / por-COF of photoresponse peroxidase based on COFs (Covalent Organic Frameworks), and the core-shell material CeO2 (at) FePC / por-COF is used for preparing the photoresponse peroxidase based on COFs. According to the invention, the highly ordered structural characteristic of the COFs material is utilized, so that the problem of fluorescence quenching caused by aggregation of traditional micromolecule photosensitizers (such as porphyrin, phthalocyanine and the like) in the use process is effectively solved. According to the present invention, the photoresponsive peroxidase activity is significant, and the chromogenic substrate of tetramethyl benzidine (TMB) can be effectively catalyzed; fePc is integrated into a COFs structure, so that the photoresponse activity of the COFs under near-infrared 808 nm is enhanced, and the COFs have excellent reactive oxygen species (ROS) generation capacity, and have great potential in the aspects of photodynamic therapy, antibacterial treatment, cell imaging and the like in biomedical application.

The invention discloses a method for detecting 17 beta-estradiol by using a colorimetric-fluorescent dual-mode biosensor, and belongs to the technical field of chemical analysis and detection. According to the method, a colorimetric-fluorescent dual-mode biosensor for detecting 17 beta-estradiol is established based on oxidase-like activity and fluorescence quenching characteristics of manganese dioxide nanosheets and catalytic characteristics of alkaline phosphatase by taking a nucleic acid aptamer as a recognition molecule. The synthesis cost of the nucleic acid aptamer is low, the method has universality, and other target objects can be detected by changing the nucleic acid aptamer; mBs-DNA-ALP is prepared by using a biotin-streptavidin reaction and a DNA hybridization reaction, and a structure conversion process from double-stranded DNA to an aptamer / target compound is realized by using targeted induction, so that a detection signal is amplified; according to the method for detecting 17 beta-estradiol through colorimetric-fluorescence signals, dual-mode output is achieved, results are verified and supplemented mutually, and the specificity and accuracy of the method are improved.

The invention belongs to the technical field of fluorescent probes, and discloses a fluorescent probe based on BODIPY targeted lipid droplets and application of the fluorescent probe. The structural formula of the fluorescent probe for targeting the lipid droplets is as follows: # imgabs0 # is as follows: due to strong electron withdrawing capability and relatively large steric hindrance of ester groups, the ester groups of the probe can rotate in an excited state, so that energy is lost through non-radiative transition, and fluorescence quenching is caused. The ester group of the probe can rotate in an excited state due to the strong electron withdrawing capability and large steric hindrance of the ester group, so that energy is lost through non-radiative transition, and fluorescence quenching is caused. And due to the introduction of the ester group, the fat solubility of the BODIPY dye is further enhanced, so that the lipid droplet targeting property of the probe is effectively improved, and no-wash imaging of lipid droplets is realized.

The invention provides a pathogen viable bacteria detection kit based on endogenous protein activated deoxyribozyme. The kit comprises a deoxyribozyme recognition element and a signal transduction element. The deoxyribozyme recognition element is composed of a substrate chain and an enzyme chain, and the signal transduction element is composed of two DNA reporter probes (Sub-F and Sub-Q), wherein the tail ends of the two DNA reporter probes are respectively marked with a fluorescence staining group and a fluorescence quenching group. The deoxyribozyme recognition element can recognize specific endogenous protein of target pathogenic viable bacteria and activate an enzyme chain to cut a substrate chain to generate two independent substrate chain fragments. And then a signal transduction element is introduced to realize specific output of a fluorescence signal. The kit provided by the invention can realize detection of living salmonella by targeting endogenous protein, can sensitively detect 190 CFU / mL of living salmonella with abundance as low as 0.1%, and has a good application prospect in the directions of food safety detection and clinical examination.

The invention relates to the field of viscosity detection, and provides a fluorescent probe for detecting the viscosity of mask essence as well as a preparation method and application of the fluorescent probe. 4, 4 '-(iminomethylene) bis (N, N-dimethylaniline) which is slightly soluble in water reacts with acid to form auramine hydrochloride, the water solubility of the auramine hydrochloride fluorescent probe is greatly enhanced, the probe in a water phase is converted into heat (non-radiation decay) due to twisted intramolecular charge transfer and excited state energy caused by benzene ring rotation, and fluorescence quenching is shown; when the viscosity of the solvent is increased, rotation of the benzene ring is blocked, excited state energy cannot be converted into heat and is dissipated in a radiation attenuation mode, fluorescence is enhanced, and viscosity detection of the mask essence with the main component being water is achieved based on the principle. According to the present invention, the method has characteristics of simple synthesis, stable property, no interference of solvent polarity during detection, long-term storage and long-term use, rapid and high-specificity viscosity response ability, and quantitative analysis of viscosity.

The invention provides a method for screening a hyaluronidase (HAase) inhibitor, and belongs to the technical field of medicines. The method comprises the following steps: firstly, rapidly identifying a natural product with HAase inhibition activity by adopting a fluorescence sensing technology according to the principle that carbon quantum dots (CDs) are used as a fluorescence probe, hyaluronic acid (HA) modified gold nanoparticles are used as a quencher, and the fluorescence of the CDs can be quenched by the quencher; when HAase exists, HA can be enzymatically decomposed to release CDs, and fluorescence recovery is caused; when a screened sample has HAase inhibition activity, CDs fluorescence recovery can be prevented. And 2, capturing components in a sample capable of inhibiting HAase activity by virtue of an affinity chromatography technology, immobilizing HAase on a carrier material by virtue of a method, eluting by virtue of a solvent to obtain components capable of being combined with HAase, and identifying the components by virtue of a liquid chromatography-mass spectrometry technology. According to the method, the HAase inhibitor can be rapidly and accurately screened from natural products.

The invention relates to the technical field of water quality detection, in particular to a method for evaluating the toxicity level of halogenated disinfection byproducts based on protease. According to the invention, fluorescence spectrum, synchronous fluorescence spectrum and ultraviolet absorption spectrum technologies are adopted to research the interaction of seven cyclic and heterocyclic halogenated disinfection byproducts with pepsin and trypsin. A fluorescence quenching mechanism is analyzed through a Stern-Volmer equation, a double logarithmic equation and the like, and the influence of the halogenated disinfection by-product on the conformation of the protease is disclosed in combination with constants and thermodynamic parameters. The binding force of the halogenated disinfection by-product and protease is in positive correlation with cytotoxicity, a new index is provided for toxicity evaluation of the halogenated disinfection by-product, and optimization of a drinking water disinfection process and formulation of a water quality standard are facilitated.

A method for manufacturing display panel including: forming pixel defining layer on one side of base substrate; forming quantum dot layer, including sequentially forming first quantum dot pattern of at least two quantum dot patterns, forming first quantum dot pattern of one quantum dot pattern including forming quantum dot film capable of emitting light with corresponding colors, quantum dot film including quantum dot body and cross-linkable ligand; performing local cross-linking process on quantum dot film; performing developing process on quantum dot film subjected to local crosslinking process to form first quantum dot pattern in pixel accommodating hole corresponding to corresponding color; after forming first quantum dot pattern of quantum dot patterns, the method includes performing fluorescence property quenching process to quench fluorescence of quantum dot body in quantum dot on surface of first quantum dot pattern and not subjected to crosslinking process. Also provided is display panel and display device.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to a fluorescent carbon dot based on p-phenylenediamine and triphenylamine as well as a preparation method and application of the fluorescent carbon dot. The fluorescent carbon dots are obtained by taking triphenylamine and p-phenylenediamine as raw materials through a solvothermal method. The invention provides a functionalized carbon dot based on p-phenylenediamine and triphenylamine. The functionalized carbon dot can achieve 599 nm emission under excitation of the wavelength of 365 nm. NADH and active sites on the surfaces of the carbon dots form a non-fluorescent ground state compound through a static quenching mechanism, 599 nm fluorescence quenching and 444 nm emission enhancement are caused, and ratio fluorescence detection of NADH is achieved. The method is good in linear relation, high in selectivity, rapid in response and high in sensitivity, and the detection limit is as low as 108 nM.

The invention belongs to the technical field of nano-particles, and particularly discloses fluorescent polyethylene glycol terephthalate nano-particles and a preparation method thereof. The preparation method comprises the following steps: crushing and alkali-washing a raw material containing polyethylene glycol terephthalate in sequence to obtain polyethylene glycol terephthalate particles; mixing the mixed solution with an organic solvent to obtain a mixed solution, mixing the mixed solution with a polyethylene glycol terephthalate particle poor solvent, and filtering to obtain polyethylene glycol terephthalate nanoparticles; the nano-particles are mixed with tween-20, and nano-particle dispersion liquid is obtained; and carrying out swelling reaction on the nano-particle dispersion liquid and a tetraphenyl ethylene solution to obtain the fluorescent polyethylene glycol terephthalate nano-particles. According to the invention, the particle size and shape of the nanoparticles can be accurately controlled, the consistency of products is improved, and the phenomena of sedimentation and aggregation are reduced. By controlling the aggregation state of molecules, fluorescence quenching is effectively avoided, and the defect that traditional organic fluorescence aggregation is easy to quench is overcome.

The invention discloses application of a dual-emission Eu < 3 + > and Cd-MOF material in detection of polluting anions. Experimental results show that the material has an obvious fluorescence quenching effect on CrO4 < 2-> and Cr2O7 < 2->. When the amount of the added CrO4 < 2-> is 2.0 [mu] mol, the fluorescence intensity of the Eu < 3 + > Cd-MOF is quenched to 93.15% (500 nm) and 94.51% (620 nm); similarly, when the addition amount of Cr2O7 < 2-> is 2.0 umol, the fluorescence intensity of the fluorescent powder is quenched to 86.25% (500 nm) and 83.31% (620 nm). Therefore, the dual-emission Eu < 3 + > and Cd-MOF can selectively identify CrO4 < 2-> and Cr2O7 < 2-> ions, and has the advantages of rapidness, simplicity, convenience, sensitivity, accuracy and the like.

The application belongs to the technical field of fluorescent probe detection, and discloses a bimetallic nanocluster fluorescent probe compound and application thereof in preparation of a product for rapidly detecting acetylene. The fluorescent probe compound is a gold-copper bimetallic nanocluster solution modified by adamantane thiol, which is used as a fluorescent probe for acetylene detection. The solution has a clear and controllable preparation process. Through specific proportioning and reaction conditions of cuprous chloride, adamantane thiol, tetrachloroauric acid, sodium borohydride and other reagents, a probe solution with stable fluorescence characteristics can be prepared. The bimetallic nanocluster fluorescent probe compound is applied in preparation of a product for rapidly detecting acetylene. Through specific combination of fluorescent molecules and acetylene gas to trigger the secondary effect of fluorescence quenching, detection is realized, and other substances cannot trigger quenching. At a wavelength of 700 nm, the linear relationship (R 2 >0.99) between the fluorescence intensity and the acetylene concentration realizes accurate quantification of the acetylene concentration, and the minimum detection limit is 0.026 muL / L.

The present invention discloses a compound dye for fluorescent staining of cells in complex matrices. The compound dye comprises, based on a total volume of 100 mL, a buffer system having a pH of 6.0 to 8.0; a fluorescent dye, wherein the fluorescent dye is 2× to 40× Sybr Gold or 2 to 80 mg of propidium iodide; 0.1 to 10 mL of Triton X-100; 0 to 24 mL of glycerol; 0 to 800 mg of EDTA-disodium; and a polyamide-amine dendrimer. The two preferred compound fluorescent dye formulas of the present invention can both achieve efficient staining of cells in complex matrices and accurate counting of their number, and the dye has excellent fluorescence color development ability. Compared with other dyes, the compound dye has a fast staining rate (staining can be achieved within 30 seconds), stable staining, and is not prone to fluorescence quenching under long-term illumination.

The application discloses an up-conversion fluorescent probe, an isoniazid detection method and a portable isoniazid detection sensor and belongs to the field of biological detection. The up-conversion fluorescent probe is composed of lanthanide-doped core-shell structure up-conversion nanoparticles subjected to acid pickling treatment and p-dimethylaminobenzaldehyde, wherein the core particle diameter of the up-conversion nanoparticles is 25.85-28.85 nm, and the shell layer thickness is 0.98-1.38 nm. The lanthanide-doped core-shell structure up-conversion nanoparticles are used as an energy donor, and the p-dimethylaminobenzaldehyde is used as a specific recognition molecule, and ratio-type fluorescence detection is realized through an inner filter effect. Isoniazid reacts with DMAAB to generate a Schiff base, which causes fluorescence quenching at 475 nm and no change in 655 nm fluorescence, so that the fluorescence color changes from blue to red. Due to the near-infrared excitation characteristics of the up-conversion nanoparticles, the up-conversion nanoparticles are not easily disturbed by background fluorescence, so that rapid quantitative detection of isoniazid in whole blood is realized, and the detection limit is as low as 2.4 μm. The application is simple in operation, strong in anti-interference and suitable for clinical individualized drug monitoring.

The invention belongs to the technical field of chrysin detection, and particularly relates to a dual-emission fluorescent molecularly imprinted polymer as well as a preparation method and application thereof. The method comprises the following steps: preparing carbon dots with different emission wavelengths; carrying out amination modification on the carbon dots; and preparing the dual-emission fluorescent molecularly imprinted polymer. The method also comprises the following steps: respectively adding the dual-emission fluorescent molecularly imprinted polymer into chrysin solutions with different known concentrations, carrying out fluorescence quenching reaction, and establishing a standard curve according to the linear relationship between the detected fluorescence intensity and the corresponding concentration of the chrysin solution; adding the dual-emission fluorescent molecularly imprinted polymer into a solution of a to-be-detected product, and carrying out fluorescence quenching reaction to obtain fluorescence intensity; and calculating the concentration of chrysin in the solution to be detected according to the standard curve. The preparation method is used for solving the technical problems that in the prior art, a single-emission fluorescent molecularly imprinted polymer has defects in the aspects of sensitivity, background fluorescence interference resistance in a complex environment and the like in the detection process.

This invention provides a method for screening β-galactosidase inhibitors, belonging to the field of pharmaceutical technology. The first step of this method uses fluorescence sensing technology to rapidly identify natural products with β-Gal inhibitory activity. The principle is based on the fluorescence quenching property of p-nitrophenol (PNP) on sulfur quantum dots (SQDs); β-Gal catalyzes the hydrolysis of p-nitrophenyl-β-D-galactopyranoside (PNPG) to generate the specific product PNP; in the presence of β-Gal, PNPG can be enzymatically catalyzed to generate PNP, quenching the fluorescence of SQDs; when screened for β-Gal inhibitory activity, the fluorescence of SQDs can be restored. The second step uses affinity chromatography to capture components in samples with β-Gal inhibitory activity. This method involves immobilizing β-Gal on a support material, eluting with a solvent to obtain components that can bind to β-Gal, and then using liquid chromatography-mass spectrometry (LC-MS) to identify the components. This method can rapidly and accurately screen β-Gal inhibitors from natural products.

The invention discloses a preparation method of a dual-response fluorescent probe based on an isatin hydrazone-pyrazole Schiff base structure, the dual-response fluorescent probe is synthesized by carrying out nucleophilic addition-elimination reaction on amino-functionalized isatin hydrazone and aldehyde group modified pyrazole, and the chemical structural formula of the dual-response fluorescent probe is as follows: # imgabs0 #. The probe disclosed by the invention has high specific recognition capability on Cu < 2 + > and CN <->. The probe shows fluorescence quenching response to Cu < 2 + >, the response time is short, and the anti-interference capability is strong. The probe realizes selective recognition of CN <-> in a fluorescence'opening 'mode, and shows a dual recognition signal of CN <-> along with obvious solution color change.

The invention relates to a method for specifically detecting tetrahydrocannabinol and hydride thereof by utilizing bridged beta-cyclodextrin, and belongs to the technical field of analysis and detection of supramolecular materials. The method comprises the following steps: firstly, combining 1, 6-hexamethylenediamine bridged bis-beta-cyclodextrin with an indicator to form a supramolecular probe; in a buffer system with a pH value of 9-11, tetrahydrocannabinol or a hydride thereof specifically competes to be combined with a cavity and displaces an indicator by utilizing a synergistic inclusion effect of a bridging subject and dissociation difference of guest molecules, so that the system is subjected to fluorescence quenching or color change, and a structural analogue cannabidiol does not generate obvious interference. The method solves the problems that the sensitivity is low and cannabidiol cannot be effectively distinguished in the prior art through cooperation of specific main body construction and environment regulation, has the advantages of being rapid in detection, high in specificity, high in accuracy and the like, and is suitable for environment monitoring and field screening.

The invention relates to the technical field of photovoltaic modules, and particularly discloses a tetraphenyl ethylene-benzotriazole derivative compound and application thereof in the photovoltaic field. The absorption spectrum of the tetraphenyl ethylene-benzotriazole derivative compound disclosed by the invention can be subjected to red shift to 420 nm, so that high-energy ultraviolet light can be completely absorbed; the maximum emission wavelength of a fluorescence emission spectrum is more than 440nm and even can reach 500nm, so that the light utilization level of the crystalline silicon solar cell is improved; the absolute light-emitting quantum efficiency is very high, and the converted wavelength can be better utilized by a photovoltaic device. The substituted tetraphenyl ethylene part contained in the derivative compound has AIE performance, and the fluorescence quenching effect of traditional small organic molecules in high concentration, solid state and aggregation state is avoided. The light conversion film can overcome the problem that the fluorescence quantum yield is remarkably reduced in the laminating process, the performance of the film is not affected when the phenomena of precipitation, agglomeration and the like of auxiliaries in the film occur, and the light conversion film is used for preparing a photovoltaic module.

The present invention provides methods for quantifying and / or screening for the formation of complexes, in which two of the three compounds of the complex are proteins, one of the two tagged with a fluorescent label and the other of the two tagged with a fluorescence quencher. The third compound is a compound capable of linking those two proteins together, thus forming a ternary complex. The methods of the present invention consist of analysing the changes in fluorescence spectrum that occur following the formation of a complex comprising said compounds, due to the interaction between fluorescent label and fluorescence quencher that formation of said complex brings.

The invention relates to the technical field of biological medicine, in particular to a TREM-1 targeted fluorescent molecular probe as well as a preparation method and application thereof, and discloses a fluorescent molecular probe named as' TREM-1 targeted fluorescent molecular probe as well as a preparation method and application thereof '. The invention aims to solve the problems of large molecular weight, poor pharmacokinetic performance, limited tissue penetration, high imaging background and poor imaging timeliness of the existing antibody fluorescent probe. The probe comprises a TREM-1 targeting module (TDM), a near-infrared fluorescence reporter group, a dark quenching group and a conformational sensitive connecting arm (CSL). The fluorescence of the probe is quenched before the probe is combined with TREM-1, and after the probe is combined with a target spot, TDM specific binding induces CSL conformation rearrangement, so that a reporter group and a quenching group are spatially separated, fluorescence resonance energy transfer (FRET) is relieved, and fluorescence activation is realized. According to the invention, rapid, high-contrast and low-background fluorescence imaging of atherosclerotic plaques is realized, and the probe is small in molecular size, strong in tissue penetrability and rapid in-vivo clearing.

The invention discloses a preparation method of an Eu-Tb-In-MOF material based on a dual fluorescence emission source type, and the preparation method specifically comprises the following steps: under a closed condition, uniformly mixing terbium nitrate hexahydrate, europium nitrate hexahydrate, indium nitrate pentahydrate, an organic ligand, N, N-dimethylformamide and acetonitrile, then adjusting the pH value of a mixed solution, and carrying out solvothermal reaction, washing, filtering and drying to obtain the Eu-Tb-In-MOF material. The invention also discloses an application of the Eu-Tb-In-MOF material in identification of heavy metal ions Cr in a water environment. The Cr (VI) ions in the water environment are detected through fluorescence quenching sensing, excellent high selectivity is shown, and interference of other single and mixed anions and cations in the water environment can be resisted.

This invention belongs to the field of compound technology and provides a DNA-intercalating fluorescent quenching compound, its preparation method, and its application. This invention provides a DNA-intercalating fluorescent quenching compound that exhibits absorption in the visible light region and certain DNA sequence selectivity. The DNA-intercalating fluorescent quenching compound provided by this invention can be further used to design fluorescence analysis systems for restriction endonuclease EcoRV and DNases. The DNA-intercalating quenching compound can achieve relevant fluorescence analysis by combining with fluorescent molecules in a covalent-free manner, reducing the need for chemical labeling steps, thereby improving efficiency and reducing detection costs, and showing good application prospects in related analyses.

The invention discloses an aptamer sensor for detecting a novel psychoactive substance ketamine and application of the aptamer sensor. Belongs to the technical field of poison detection. The aptamer sensor for detecting the novel psychoactive substance ketamine is successfully established by taking a two-dimensional nano material as a fluorescence quencher and a carrier, chemically coupling the two-dimensional nano material with a double-chain nucleic acid aptamer through a liquid-phase amidation reaction and carrying out surface passivation treatment, so that the chemical coupling efficiency is greatly improved; and the stability and the sensitivity of the sensor are enhanced. In addition, when the sensor is introduced into complex biological matrixes such as blood, urine and saliva, the system does not display non-specific signals, and compared with a physical adsorption system, the KET in complex biological fluid can be reliably detected, and a new thought and method are provided for reliable detection of other novel psychoactive substances.

The application discloses a fluorescence enhancement anchor agent based on a fluoroboron dipyrrin-tetrazine dye for inflation microscopy technology, and belongs to the technical field of biochemical analysis. The fluorophore of the fluorescence enhancement anchor agent is shown in formula (I), formula (II) or formula (III): the tetrazine group carried by the fluorescence enhancement anchor agent is removed from fluorescence quenching through a biological orthogonal reaction with a drug probe, and the drug probe is shown in formula (IV): the fluorescence enhancement anchor agent can covalently and non-covalently anchor small molecules in a hydrogel network based on a tetrazine biological orthogonal reaction, and realizes inflation microscopic fluorescence imaging of a double fluorescence enhancement mechanism. In addition, the drug probe selects a non-covalent drug, gefitinib, as a probe core, is modified with a BCN click handle, and successfully realizes in-situ fluorescence inflation microscopic imaging of an EGFR drug-target, thereby providing a new tool for research and clinical research on non-covalent drug target co-localization information of exosomes.

The invention provides a light-emitting auxiliary material and a preparation method and application thereof, and belongs to the technical field of light-emitting materials, the structural formula of the light-emitting auxiliary material is shown in the general formula I. The light-emitting auxiliary material takes 9, 9-phenylalkyl fluorene as a parent nucleus, aryl and arylamine groups are connected to the two sides of fluorene of the 9, 9-phenylalkyl fluorene respectively, 9-phenylalkyl fluorene has relatively high glass transition temperature and quantum fluorescence efficiency, the stability and luminous efficiency of the compound can be effectively improved, the energy levels of the highest occupied molecular orbital and the lowest unoccupied molecular orbital can be flexibly adjusted by introducing an electron-donating group arylamine, and the energy barrier of hole injection can be effectively reduced; meanwhile, the other side is connected with an aryl group, so that a conjugated system can be effectively extended, a fluorescence quenching phenomenon caused by intermolecular pi-pi accumulation and excessive molecular aggregation is reduced, the service life of an OLED (Organic Light Emitting Diode) device can be effectively prolonged, the luminous efficiency of the OLED device can be effectively improved, and the driving voltage can be reduced by utilizing the light-emitting auxiliary material.