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687 results about "Fluorescent quenching" patented technology
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Carbon nano-dot, and preparation method and application thereof
ActiveCN102849722AOvercome costsOvercoming the problem of easy fluorescence quenching in the aggregated stateNanotechnologyNano-carbonBiological imagingOrganic compound
The invention discloses a carbon nano-dot, and a preparation method and an application thereof, and solves a problem that the application of present nano-dots is restricted because of high preparation cost and easy fluorescent quenching appearance of an aggregate state. According to the invention, the carbon nano-dot having a high fluorescence quantum efficiency is prepared through adopting a polycarboxyl or polyhydroxy contained organic compound, or an amino acid as a raw material, and urea as a surface passivation modification agent, and through a microwave process, and a carbon nano-dot fluorescent ink is prepared through using the carbon nano-dot. The preparation method disclosed in the invention has the advantages of simplicity, low cost and convenient large-scale production; the fluorescent quenching of the prepared carbon nano-dot on the surface of a biological product does not appear, and the highest fluorescence quantum efficiency is 42%; and the prepared carbon nano-dot fluorescent ink is nontoxic, does not generate a precipitate after long-time dispose, and can be applied to the biological imaging field, the biological product identification field, the information storage field, the information encryption field, the false proof field, the illumination display field, the photovoltaic device field and the like.
Owner:CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
Method and apparatus for analyzing individual cells or particulates using fluorescent quenching and/or bleaching
InactiveUS8081303B2Suitable for useBioreactor/fermenter combinationsBiological substance pretreatmentsParticulatesOptical density
A method for analyzing a blood sample is provided that includes the steps of: a) providing a blood sample having one or more first constituents and one or more second constituents, which second constituents are different from the first constituents; b) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by a first panel and a second panel, both of which panels are transparent; c) admixing a colorant with the sample, which colorant is operative to cause the first constituents and second constituents to fluoresce upon exposure to predetermined first wavelengths of light, and which colorant is operative to absorb light at one or more predetermined second wavelengths of light; d) illuminating at least a portion of the sample containing the first constituents and the second constituents at the first wavelengths and at the second wavelengths; e) imaging the at least a portion of the sample, including producing image signals indicative of fluorescent emissions from the first constituents and the second constituents and the optical density of the first constituents and the second constituents; f) determining a fluorescence value for each the first constituents and second constituents using the image signals; g) determining an optical density value for each of the first constituents and second constituents, which optical density is a function of the colorant absorbed by the constituents, using the image signals; and h) identifying the first constituents and the second constituents using the determined fluorescence and optical density values.
Owner:ABBOTT POINT CARE
Characterization of biopolymers by resonance tunneling and fluorescence quenching
InactiveUS20060019259A1Bioreactor/fermenter combinationsBiological substance pretreatmentsFluorescenceBiopolymer
The present invention provides a method and apparatus for determining the identity of a monomeric residue of a biopolymer. The apparatus comprises a substrate having a nanopore, a potential-producing element for producing a ramped potential across electrodes adjacent to the nanopore, and a quenchable excitable moiety adjacent to the nanopore. As a biopolymer passes through the nanopore, the identity of monomeric residues of a biopolymer may be determined by detecting changes in (a) current across the electrodes and (b) a signal of the quenchable excitable molecule. The subject method and apparatus find use in determining the identity of a plurality of monomeric residues of a biopolymer, and, as such, may be employed in a variety of diagnostic and research applications.
Owner:AGILENT TECH INC
Intramolecularly-quenched near infrared fluorescent probes
InactiveUS20060275775A1Increases target/background ratioRaise the ratioUltrasonic/sonic/infrasonic diagnosticsSurgeryEpicoccononeIn vivo
An intramolecularly-quenched, near infrared fluorescence probe that emits substantial fluorescence only after interaction with a target tissue (i.e., activation) is disclosed. The probe includes a polymeric backbone and a plurality of near infrared fluorochromes covalently linked to the backbone at fluorescence-quenching interaction-permissive positions separable by enzymatic cleavage at fluorescence activation sites. The probe optionally includes protective chains or fluorochrome spacers, or both. Also disclosed are methods of using the intramolecularly-quenched, near infrared fluorescence probes for in vivo optical imaging.
Owner:THE GENERAL HOSPITAL CORP
Multifunctional nuclear shell structure drug carrier material and preparation method thereof
InactiveCN101670107AIncrease temperatureHigh purityInorganic non-active ingredientsGranular deliveryMagnetite NanoparticlesRare earth
Owner:如皋市生产力促进中心
Nitrogen-doped carbon nanometer particle as well as preparation method and application thereof
InactiveCN103113886AOvercoming the technical problem of easy fluorescence quenchingEasy to prepareNon-macromolecular adhesive additivesInksMicrowave methodSolvent
The invention discloses a nitrogen-doped carbon nanometer particle as well as a preparation method and application thereof, belongs to the field of nanometer material science and is used for solving the technical problems that fluorescence quenching is easily caused to the aggregative state of the carbon nanometer particle due to surface passivation modifier which is added for the preparation of existing carbon nanometer particles. The nitrogen-doped carbon nanometer particle is prepared through a microwave method by using organic compounds containing polycarboxyl or polyhydroxy as materials and using ammonia water as a solvent and a nitrogen doping source. The invention further provides the application of the nitrogen-doped carbon nanometer particle as fluorescent ink and fluorescent glue. The preparation method disclosed by the invention is simple, low in cost, and convenient to realize large-scale production; the maximal fluorescent quantum efficiency of the solid film formed by the prepared fluorescent glue is as high as 84%; the prepared fluorescent ink is non-toxic, generates no precipitates after being placed for a long time, is strong in fluorescence characteristic and can be applied to various fields such as bio-imaging, biological product identification, information storage, information encryption, counterfeiting prevention, illumination and display, sensing and photovoltaic devices.
Owner:CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
Multi-target quantum-dot mark nucleic acid chip and preparation method and detection method thereof
InactiveCN101519695AMultiple duplicate detection synchronizationEasy to makeMicrobiological testing/measurementFluorescenceBiology
The invention discloses a multi-target quantum-dot mark nucleic acid chip and a preparation method and a detection method thereof, wherein the nucleic acid chip comprises a solid phase holder and an oligonucleotide probe array fixed on the surface of the solid phase holder, wherein the oligonucleotide probe array comprises at least two oligonucleotide probes which do not contain self-complementary sequences; one end of the oligonucleotide probe marks quantum dots and is fixed by the quantum dots, and the oligonucleotide probes with different sequences are marked by the quantum dots which emit fluorescence with different wavelengths; or the oligonucleotide probe array comprises at least two molecular beacons, wherein one end of the molecular beacon marks quantum dots and is fixed by the quantum dots, the molecular beacons with different sequences are marked by the quantum dots which emit fluorescent with different wavelengths, and the other end of the molecular beacon is marked by a fluorescence quenching group. By utilizing the basic-group complementation pairing principle and the FRET phenomenon, the detection of a plurality of special nucleic acid sequences in a nucleic acid sample to be detected can be simultaneously achieved; in addition, the invention has simple preparation and accurate, sensitive, simple, convenient and rapid detection and can detect a plurality of samples simultaneously.
Owner:THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
Detection of chemical ligation using fluorescence quenching leaving groups
InactiveUS20040259102A1Sugar derivativesMicrobiological testing/measurementChemical ligationLeaving group
Novel compounds having a fluorescence quencher as a leaving group are disclosed. Nucleic acids and other molecules containing a fluorophore and a fluorescence quencher are disclosed as an embodiment of this invention. The use of the oligonucleotides in enzyme-free oligonucleotide ligation reactions results in an increase in fluorescence when the oligonucleotide also contains a nearby fluorophore. The ligation reactions can be performed in solution, on surfaces, or in cells.
Owner:SANDFORD UNIV +1
Carbon dots, preparation method therefor, carbon-dot composite material, preparation method therefor and luminescent LED (Light Emitting Diode)
ActiveCN106867528AImprove quantum efficiencyInhibition quenchingNanoopticsLuminescent compositionsSilicon dioxideLight-emitting diode
The invention provides a preparation method for carbon dots. The preparation method comprises the steps: mixing citric acid and urea in a solvent, and then, carrying out heating, so as to obtain a reaction solution; and carrying out centrifugal separation on the reaction solution, so as to obtain precipitates, i.e., the carbon dots, wherein the solvent is one or a mixture of two of water, glycerine and dimethylformamide. According to method, visible-light-all-waveband-luminescent carbon dots are prepared through adopting different solvents, and thus, the carbon-dot composite material provided by the invention can emit light in all wave band of visible light, particularly, white-light carbon-dot composite materials of different color coordinates and color temperatures can be obtained by adopting the carbon dots of different colors. Furthermore, the carbon-dot composite material provided by the invention employs silicon dioxide as a dispersion matrix, so that gathered induced fluorescence quenching can be inhibited, and the composite material has relatively high quantum efficiency. The invention further provides the preparation method for the carbon dots, the carbon-dot composite material, a preparation method therefor and a luminescent LED (Light Emitting Diode).
Owner:CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
Pillar[5]arene and 2-hydroxy-3-naphthoic acid complex and preparation thereof and application in detecting iron ions and fluorine ions
InactiveCN105753662AOrganic compound preparationCarboxylic compound preparationMolecular fluorescenceFluorescent quenching
The invention provides a pillar[5]arene and 2-hydroxy-3-naphthoic acid complex.Copolymerized pillar[5]arene serves as a subject, 2-hydroxy-3-naphthoic acid serves as an object, C-H......pi and electrostatic attraction serve as driving force, partial 2-hydroxy-3-naphthoic acid provided with negative charges is included into a cavity of pillararene in an alkaline environment, the assembling process of the subject and the object is completed, and the stable pillar[5]arene and 2-hydroxy-3-naphthoic acid complex is formed.Iron ions are added to an alkaline aqueous solution of the complex, and the subject and the object are subjected to fluorescence quenching; fluorine ions are added, a complex is formed by the fluorine ions and the iron ions, so that the iron ions are separated from the cavity of copolymerized pillar[5]arene, and molecular fluorescence of the complex is reopened.Therefore, Fe<3+> can be determined through the pillar[5]arene and 2-hydroxy-3-naphthoic acid complex, and F<-> can be continuously identified through fluorescence quenching of the solution.
![Pillar[5]arene and 2-hydroxy-3-naphthoic acid complex and preparation thereof and application in detecting iron ions and fluorine ions](https://images-eureka.patsnap.com/patent_img/9701a422-70b5-4870-9f49-92bcf764c7de/160401111727.PNG "Pillar[5]arene and 2-hydroxy-3-naphthoic acid complex and preparation thereof and application in detecting iron ions and fluorine ions")
![Pillar[5]arene and 2-hydroxy-3-naphthoic acid complex and preparation thereof and application in detecting iron ions and fluorine ions](https://images-eureka.patsnap.com/patent_img/9701a422-70b5-4870-9f49-92bcf764c7de/160401111731.PNG "Pillar[5]arene and 2-hydroxy-3-naphthoic acid complex and preparation thereof and application in detecting iron ions and fluorine ions")
![Pillar[5]arene and 2-hydroxy-3-naphthoic acid complex and preparation thereof and application in detecting iron ions and fluorine ions](https://images-eureka.patsnap.com/patent_img/9701a422-70b5-4870-9f49-92bcf764c7de/160401111735.PNG "Pillar[5]arene and 2-hydroxy-3-naphthoic acid complex and preparation thereof and application in detecting iron ions and fluorine ions")
Owner:NORTHWEST NORMAL UNIVERSITY
Application of luminous metal organic framework material in detecting nitro aromatic hydrocarbon pollutant
InactiveCN104447377ARealize detectionEasy to prepareOrganic compound preparationAmino-carboxyl compound preparationMetal-organic frameworkUltimate tensile strength
The invention relates to an application of a luminous metal organic framework material in detecting a nitro aromatic hydrocarbon pollutant. The MOFs (Metal-Organic Frameworks) as a fluorescent sensing material can fast, easily, conveniently and sensitively realize the detection on a trace amount of nitro aromatic hydrocarbon, is gradually quenched in fluorescence intensity with the content increase of the nitro aromatic hydrocarbon pollutant and achieves different fluorescent quenching degree on different nitro aromatic hydrocarbon compounds. Thus, the luminous metal-organic framework material has potential application prospect on the aspects of detection of an exploder and an environment and the like.
Owner:TIANJIN POLYTECHNIC UNIV
Azulene dimer-quenched, near-infrared fluorescent probes
InactiveUS20060147378A1Reduced background fluorescenceFeasibility of simultaneous multiple probe useUltrasonic/sonic/infrasonic diagnosticsMethine/polymethine dyesEpicoccononeIn vivo
An intramolecularly-quenched, near-infrared fluorescence probe that emits substantial fluorescence only after interaction with a target tissue (i.e., activation) is disclosed. The probe includes a polymeric backbone and a plurality of near-infrared fluorochromes covalently linked to the backbone at fluorescence-quenching interaction-permissive positions separable by enzymatic cleavage at fluorescence activation sites. The probe optionally includes protective chains or fluorochrome spacers, or bothours. Also disclosed are methods of using the intramolecularly-quenched, near-infrared fluorescence probes for in vivo optical imaging.
Owner:THE GENERAL HOSPITAL CORP
Paper chip, making method thereof, and bio-molecule detection method
ActiveCN104807987AReduce dosageQuick responseBiological testingFluorescence/phosphorescenceSurface markerOrganic dye
A paper chip, a preparation method thereof and a detection method for biological molecules are provided. The paper chip includes a paper-based material, a fluorescent donor and a fluorescent quenching agent. A detection zone is arranged on the paper-based material, and the fluorescent donor material is secured within the detection zone of the paper-based material. The fluorescent donor material includes an up-conversion luminescence nano-material and a surface marker marked on the up-conversion luminescence nano-material. The fluorescence quenching agent includes an organic dye or a fluorescent receptor. The combination of the up-conversion luminescence analysis and the paper chip technology decreases the amount of reactants used, reduces testing costs, speeds up the reaction rate of the reactants, and increases the possibility of applying the paper chips to the clinical testing.
Owner:GUANGZHOU IMPROVE MEDICAL TECH CO LTD
EGFR gene 20 exon T790M and C797S mutation detection primers, probes and method
InactiveCN107083438AReduce distractionsIncrease the Tm valueMicrobiological testing/measurementDNA/RNA fragmentationSpecific detectionMutation detection
The invention discloses a primer pair for specific amplification of EGFR gene 20 exon T790M and C797S loci. The sequence of the primer pair is shown in SEQ ID No:1-2. The invention further discloses a probe set for specific detection of the T790M and C797S loci. The probe set comprises a wild type probe and a mutant type probe, and the sequences are shown in SEQ ID No:3-7. The invention further discloses a reagent kit comprising the primer pair and the probe set, and a method for adopting the primer pair and the detection set for detecting EGFR gene 20 exon T790M and C797S mutation. By designing the specific primers and probes, the 3' end of the probes is connected with a minor groove binder and a non-fluorescent quenching group, the interference of background signals is lowered, hybridization of the probes and a template is stabilized, a Tm value of the probes is increased, and therefore the mutation detection sensitivity is improved.
Owner:上海捷易生物科技有限公司
Fluorescent quenching detection reagents and methods
InactiveUS20060292589A1Improved UV spectral overlapIncreased signal noiseMonoazo dyesSugar derivativesMinor grooveFluorescent quenching
Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group.
Owner:ELITECHGRP +2
Assay procedure using fluorogenic tracers
Fluorescent energy transfer dyes capable of moving between a more stacked configuration to exhibit fluorescent quenching and a more spaced configuration to exhibit fluorescence can be conjugated to a peptide epitope or nucleic acid for use in the detection of an unknown antibody in bulk solution. The resulting labeled peptide reagent can be used in an immunoassay procedure by placing it in bulk solution along with the unknown antibody to be detected. When the antibody binds to the peptide epitope, the pair of dyes carried by the peptide epitope will have their configuration altered from a stacked to an unstacked configuration and will exhibit a fluorescent increase in response thereto.
Owner:UNIV OF UTAH RES FOUND
Preparation method of quantum dot@Cu nano-cluster ratiometric fluorescent sensor and application thereof in Cu<2+> detection
InactiveCN104745194AFix interferenceHigh precisionFluorescence/phosphorescenceLuminescent compositionsFluoProbesFluorescent quenching
The invention discloses a preparation method of a quantum dot@Cu nano-cluster ratiometric fluorescent sensor and application thereof in Cu<2+> detection, belonging to the technical field of fluorescence sensing. According to the preparation method, CuNCs prepared by taking polyethyleneiminen as a template is coupled to the surface of silicon dioxide coated CdSe QDs to prepare CdSe QDs@CuNCs ratiometric fluorescent sensor having CdSe QDs and CuNCs double-fluorescence emission signals. When Cu<2+> exists in a solution, amino of polyethyleneimine and Cu<2+> are subjected to complexing to form a copper-amine complex, thereby avoiding the problem that CuNCs fluorescence quenching is caused but the fluorescence intensity of CdSe QDs is unchanged because electrons are transferred from amino to CuNCs. With the increase of Cu<2+> concentration, the intensity ratio of two fluorescence emission peaks of CdSe QDs and CuNCs is reduced gradually, thereby realizing sensitive fluorescent and visual detection on Cu<2+>.
Owner:NANCHANG UNIV
Method for recognizing quantitative chiral amino acid by using reversible nano porphyrin fluorescence sensor
ActiveCN106706591AHigh sensitivityRealize qualitative and quantitative detectionFluorescence/phosphorescenceMaterials preparationQuantum dot
The invention discloses a method for recognizing quantitative chiral amino acid by using a reversible nano porphyrin fluorescence sensor, and belongs to the technical field of nano material preparation and chemical analysis detection. The reversible nano porphyrin fluorescence sensor for specifically recognizing quantitative proline, lysine and serine chirality adopts a CdTe quantum dot as a fluorescence probe, and self-assembled porphyrin prepared from a tetra-(4-pyridyl) zinc porphyrin tetrahydrofuran solution and cetyl trimethyl ammonium bromide (CTAB) as a fluorescence quencher, and with the specific combination of the florescence probe and the fluorescence quencher, an openable / closeable nano porphyrin fluorescence sensor can be prepared. The reversible (opening-closing-opening) nano porphyrin fluorescence sensor can be prepared under the action of the openable / closeable nano porphyrin fluorescence sensor with the chiral proline, lysine and serine. Compared with a conventional chromatographic method for recognizing amino acid chirality by separating, the method disclosed by the invention has multiple advantages.
Owner:SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
Fluorescent compound and application thereof in detecting trace methylamphetamine
ActiveCN101899021AImprove luminous efficiencyThe synthesis method is simpleOrganic chemistryFluorescence/phosphorescenceFluorescent quenchingPyrene
The invention relates to a fluorescent compound and application thereof in detecting trace methylamphetamine, which is characterized in that the structural general formula of the fluorescent compound is shown in the specification, wherein R1 and R2 in the formula are respectively one fluorescent substituent group and the derivative thereof, the fluorescent substituent group comprises triphenylamine, benzene, naphthalene, anthracene, pyrene, fluorine, dinaphthalene, carbazole, benzothiophene, phenothiazinyl and rhodamine, and R3 is alkyl substituent. The steam action of the fluorescent compound provided by the invention and the methylamphetamine can carry out obvious fluorescent quenching in short time, and the methylamphetamine can be detected according to the fluorescent intensity quenching. The fluorescent compound is a novel fluorescent sensing material for detecting methylamphetamine. The detection limit reaches 100 ppb.
Owner:SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
Preparation method and application of single-particle double-emission ratiometric fluorescent probe
ActiveCN110194950AImprove stabilityRealize visual detectionMaterial nanotechnologyNanoopticsSilicon oxideQuenching
The invention discloses a preparation method and application of a single-particle double-emission ratiometric fluorescent probe. Specifically, the preparation method of the single-particle double-emission ratiometric fluorescent probe includes: firstly preparing carboxylated silicon oxide with embedded red CdTe quantum dots, and then conducting surface covalent coupling of aminated blue carbon dots to construct the double-emission ratiometric fluorescent probe. The ratiometric fluorescent probe provided by the invention combines a fluorescence quenching system constructed by gold nanoparticlesand can be used for fluorescence enhanced detection of pesticide thiram, on the basis of fluorescence resonance energy transfer between gold nanoparticles and carbon dots, blue fluorescence can quench, red fluorescence silicon oxide is used as the internal standard, after adding of thiram, as the strong bonding effect of gold nanoparticles and sulfur atoms results brings about agglomeration, blue fluorescence recovers, and the process of closing and reopening of blue fluorescence can be realized, thus realizing the detection of thiram. The method has the advantages of high sensitivity, goodselectivity, strong anti-interference ability and low detection limit, and also can be used for detection of practical samples.
Owner:中科合肥智慧农业谷有限责任公司
Reagent, detection method and application for detection of African hog cholera virus
InactiveCN109735657AShort detection timeRapid prevention and controlMicrobiological testing/measurementMicroorganism based processesAgricultural scienceRecombinase Polymerase Amplification
The invention discloses a reagent, detection method and application for the detection of African hog cholera virus. The reagent comprises a specific primer pair and a probe, upstream and downstream primers of the primer pair are respectively sequences shown in Seq ID No. 1 and 2, and the probe is a sequence shown in Seq ID No. 3 or its reverse complement sequence; in the probe sequence shown in the SEQ ID No.3, a 28th base modifies fluorescent quenching group-dT, a 31st base is replaced with a base analog, a 33rd base modifies a fluorescent group-dT, and a 3' end modifies C3 Spacer. The reagent is sensitive, specific and efficient for detection of the African hog cholera virus by recombinase polymerase amplification; compared with conventional conventional or real-time fluorescent PCR, thereagent and the detection method have short detection time and simple operation, are especially suitable for on-site testing, and are of great significance for the rapid prevention and control of theAfrican hog cholera virus and guarantee of production safety.
Owner:SHENZHEN AUDAQUE DATA TECH
Core-shell structured quantum dot composite nanocrystalline fluorescence probe and preparation method thereof
InactiveCN104726085AImprove performanceImprove luminous efficiencyMaterial nanotechnologyNanoopticsIonQuantum yield
The invention belongs to the technical field of preparation of nanometer materials and bioanalysis detection, and particularly relates to a core-shell structured quantum dot composite nanocrystalline fluorescence probe and a preparation method thereof. The invention provides the preparation method for a ZnSe quantum dot core-shell structured composite nanocrystalline. The shell of the composite nanocrystalline is a composite silicon dioxide shell containing ZnS semiconductor clusters, and by the coating of the composite silicon dioxide shell, the photochemical stability and quantum yield of quantum dots are obviously improved; meanwhile, based on the fluorescence quenching effect of the quantum dots, the fluorescence probe used for detecting trance heavy metal ions is prepared. According to the preparation method disclosed by the invention, the reaction condition is mild, the operation method is simple, the prepared core-shell structured composite nanocrystalline has good physicochemistry stability and biocompatibility, and the prepared probe has very high sensibility and good stability, and has wide application prospect in the fields of biosensors, fluorescence probes, biomarkers and the like.
Owner:UNIV OF JINAN
Lead ion fluorescent DNA (Deoxyribose Nucleic Acid) probe and fluorescent determination method for lead ion concentration
InactiveCN102586429AWill not cause secondary pollutionStrong specificityMicrobiological testing/measurementDNA/RNA fragmentationSignalling moleculesDouble strand
The invention relates to the field of detection and analysis of lead ion concentration and discloses a lead ion fluorescent DNA (Deoxyribose Nucleic Acid) probe. The lead ion fluorescent DNA probe comprises double strands formed by hybridizing 17E deoxyribozyme with a substrate strand, wherein the tail end of the substrate strand is modified by FAM (Carboxyfluorescein). The invention also discloses a fluorescent determination method for the lead ion concentration by using the probe. The fluorescent detection of the lead ion concentration of a sample to be detected is carried out by taking the lead ion fluorescent DNA probe as an identification element of a lead ion, the FAM for modifying the tail end of the substrate strand as a fluorescent signal molecule and a colloidal gold nanoparticle as a fluorescence quencher according to the fluorescent signal difference generated when the FAM and the lead ion fluorescent DNA probe are subjected to single-double-strand combination. The fluorescent determination method disclosed by the invention has the advantages of high sensitivity, favorable selectivity for the lead ion, low cost, convenience in operation, quickness in detection and the like.
Owner:ELECTROMECHANICAL PRODS DETECTING TECH CENT OF SHANGHAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU
Rate type fluorescent probe used for mercuric ion detection and preparing method thereof
InactiveCN107884376AHigh sensitivityHigh selectivityFluorescence/phosphorescenceMercuric ionFluorescence
The invention provides a rate type fluorescent probe used for mercuric ion detection. The probe is formed by dual-emitter nanometer material gold / silver nanoclusters wrapped by bovine serum albumin, and the fluorescence-emission wavelength is 400-550 nm and 580-680 nm. The dual-emitter nanometer material gold / silver nanoclusters are synthesized through a one-pot method by using the bovine serum albumin (BSA) as a reducing agent and a stabilizer. The synthesized gold / silver nanoclusters (Au / Ag NCs) have two emission peaks, wherein Ag NCs serves as a reference signal Au NCs serves as a responsesignal and is used for selective recognition of Hg2+. When the dual-emitter gold / silver nanoclusters serve as the rate type fluorescent probe, the Ag NCs fluorescent signal intensity is kept unchangedbasically, and Au NCs is selectively combined with Hg2+ to cause fluorescence quenching. The method is quick, simple, low in detection limit, wide in linear range and high in selectivity.
Owner:SICHUAN NORMAL UNIVERSITY
Preparation method of rare-earth coordination polymer fluorescence probe and application of rare-earth coordination polymer fluorescence probe in H2O2 and glucose detection
InactiveCN105949473AReduce fluorescenceFluorescence/phosphorescenceLuminescent compositionsCatalytic oxidationOxidative enzyme
The invention discloses a preparation method of a rare-earth coordination polymer fluorescence probe and an application of the rare-earth coordination polymer fluorescence probe in H2O2 and glucose detection and belongs to the technical field of optical sensing. The preparation method comprises steps as follows: triphosadenine is mixed with a Tris-HCl buffer solution containing Ce<3+>, and the rare-earth coordination polymer fluorescence probe ATP-Ce-Tris is prepared; when H2O2 is present in the solution, Ce<3+> in the ATP-Ce-Tris is oxidized into Ce<4+> by H2O2 to result in fluorescence quenching of the ATP-Ce-Tris, and accordingly, detection of H2O2 is realized; simple and sensitive detection of glucose is further realized through fluorescence quenching of the ATP-Ce-Tris due to H2O2 which is produced through catalytic oxidation of glucose by glucose oxidases.
Owner:NANCHANG UNIV
Fluorescent quenching detection reagents and methods
InactiveUS7662942B2Improve featuresWide wavelength rangeMonoazo dyesSugar derivativesMinor grooveFluorescent quenching
Owner:ELITECHGRP +2
Fluorescent quantitative PCR primers and probes for porcine epidemic diarrhea viruses
ActiveCN103509882AImprove featuresHigh sensitivityMicrobiological testing/measurementMicroorganism based processesEpidemic diarrheaFluorescent quenching
The invention discloses fluorescent quantitative PCR (polymerase chain reaction) primers and probes for porcine epidemic diarrhea viruses. An upstream primer and a downstream primer are shown as SEQIDNO: 1-2; a probe P1 is shown as SEQIDNO: 3; a probe P2 is shown as SEQIDNO: 4; the 3' end of sequences of the probe 1 and the probe 2 are combined with a fluorescent quenching group; and the 5' end of sequences of the probe 1 and the probe 2 are combined with different fluorescent reporter groups. The primers are relatively high in specificity and sensitivity, can detect out variant PEDV (porcine epidemic diarrhea viruses), and can detect and differentiate a variety of clinical samples so as to quickly differentiate epidemic PEDV strains; the operation is simple and practical.
Owner:WENS FOODSTUFF GRP CO LTD
Construction method for near infrared fluorescence energy transfer based biosensor
ActiveCN104697968AAvoid optical interferenceHigh detection sensitivityFluorescence/phosphorescenceQuenchingLabel free
The invention provides a construction method for a near infrared fluorescence energy transfer based biosensor. The method includes: subjecting a nucleic acid aptamer to covalent coupling to a fluorescent donor surface, mixing the nucleic acid aptamer coupled fluorescence donor with a label-free fluorescent receptor till fluorescence quenching of the fluorescence donor, thus obtaining the near infrared fluorescence energy transfer based biosensor. As the sensor detection window is located in a near infrared region, the background fluorescence, the background fluorescence, scattered light, light absorption and other optical interference can be well overcome, and the detection sensitivity is improved. The biosensor can be directly applied to complex biological matrix analysis.
Owner:SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
Homogenous assay for enzymatic activity
InactiveUS20050106655A1Reduce the amount of solutionLess quenchingMicrobiological testing/measurementNickel organic compoundsProteinase activityFluorescence
Owner:PIERCE BIOTECHNOLOGY
Ultramolecular gel based on functional column [5] arene and application thereof to recognition of ferric ion and L-Cys
InactiveCN108070092AEasy to detectRealize detectionFluorescence/phosphorescenceLuminescent compositionsGel basedCyclohexanol
The invention discloses an ultramolecular gelator MP5 based on naphthalimide functional column 5 arene. The gelator can form organic ultramolecular gel MP5-G with yellow aggregation induced fluorescence in a cyclohexanol solution under the action of pi-pi; the organic ultramolecular gel can be used for detecting Fe<3+> with high selectivity and super sensitivity: when ions such as Fe<3+>, Hg<2+>,Ag<+>, Ca<2+>, Cu<2+>, Co<2+>, Ni<2+>, Cd<2+>, Pb<2+>, Zn<2+>, Cr<3+> and Mg<2+> are added into the MP5-G, fluorescence is quenched only by the Fe<3+>, and stable metal gel MP5-FeG is formed. When L-Cys is added into the fluorescence-quenched metal gel, the L-Cys and the Fe<3+> are complexed to recover the pi-pi action again among the MP5-G, so that aggregation induced fluorescence appears again,and continuous reversible ultrasensitive detection of the Fe<3+> and the L-Cys is realized.
![Ultramolecular gel based on functional column [5] arene and application thereof to recognition of ferric ion and L-Cys](https://images-eureka.patsnap.com/patent_img/e0d08da8-8d26-47d8-ad9b-411774ff67f8/171120161659.png "Ultramolecular gel based on functional column [5] arene and application thereof to recognition of ferric ion and L-Cys")
![Ultramolecular gel based on functional column [5] arene and application thereof to recognition of ferric ion and L-Cys](https://images-eureka.patsnap.com/patent_img/e0d08da8-8d26-47d8-ad9b-411774ff67f8/171120161703.png "Ultramolecular gel based on functional column [5] arene and application thereof to recognition of ferric ion and L-Cys")
![Ultramolecular gel based on functional column [5] arene and application thereof to recognition of ferric ion and L-Cys](https://images-eureka.patsnap.com/patent_img/e0d08da8-8d26-47d8-ad9b-411774ff67f8/171120161707.png "Ultramolecular gel based on functional column [5] arene and application thereof to recognition of ferric ion and L-Cys")
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