13 results about "Thiram" patented technology

The invention relates to the technical field of pesticides, and discloses a seed treatment agent and application thereof. The seed treatment agent comprises an active component and an auxiliary agent, wherein the active ingredients are prepared from flonicamid, thiram and azoxystrobin. The invention also relates to application of the seed treating agent in prevention and treatment of diseases and insect pests of food crops and oil crops. The invention provides the seed treatment agent containing the three active ingredients of flonicamid, thiram and azoxystrobin, the product stability is improved, the seed treatment agent can tolerate high and low temperature intervals, meanwhile, the product suspension rate is high, the coating falling rate is low, the seed treatment safety is good, and the seed treatment agent can be subsequently used in different countries and regions and is beneficial to large-scale popularization and application. The seed treatment agent provided by the invention is simple in preparation process and simple and convenient to operate.

The invention discloses a cyclodextrin metal organic framework composite flexible SERS (Surface Enhanced Raman Scattering) substrate for instantly detecting pesticide residues as well as preparation and application thereof, a cyclodextrin metal organic framework material (CD-MOF) is used as a carrier, gold nanoparticles are synthesized in the structure of the carrier, and a gold-loaded cyclodextrin metal organic framework material (Au at CD-MOF) is obtained; then, glycerol triglycidyl ether (GTE) is used as a cross-linking agent, and a cross-linked gold-loaded cyclodextrin metal organic framework material (CL-Au and CD-MOF) is synthesized; and finally, taking filter paper as a three-dimensional template, and combining with polydimethylsiloxane (PDMS) to prepare the Paper CL-Au CD-MOF / PDMS composite flexible substrate with relatively good SERS detection performance. The composite flexible substrate has good SERS detection performance, the detection limit of thiram on the surfaces of apples is 4.2 * 10 <-8 > M, and the detection limit of thiram on the surfaces of cucumbers is 1 ppm.

The invention relates to a complex microbial inoculant for preventing and treating tobacco bacterial wilt and application of the complex microbial inoculant. Strain in the complex microbial inoculant comprises a mulberry enterobacter 65D6 strain, a Ralstonia 56D2 strain, an agrobacterium 18A6 strain, an arthrobacter uretogenes 05-507 strain and a bacillus safensis 05-2101 strain. The five specific strains are compounded and combined for use, an excellent tobacco bacterial wilt prevention and treatment effect is achieved, and experimental research shows that the disease occurrence of tobacco plants treated by the complex microbial inoculant is obviously slowed down, and the disease index is obviously lower than that of a clear water control group and that of a thiediazole copper and chloriure.copper sulfate chemical agent control group. Meanwhile, the five strains can be mutually matched and promoted, a synergistic effect is achieved in the aspect of preventing and treating tobacco bacterial wilt, the WY11 strain is further added for combined use, the effect of preventing and treating the tobacco bacterial wilt is improved, and the better effect of promoting the growth of tobacco plants can be achieved.

The invention provides a functionalized melamine sensing sponge, a preparation method thereof and a thiram colorimetric-fluorescence visual dual-mode detection method, and belongs to the technical field of food safety detection. According to the invention, thiram is specifically combined with PDA-coated CuO2 in the sensing sponge, so that the nano-enzyme catalytic activity of the sensing sponge is excited, colorless TMB is catalyzed to be oxidized into blue oxTMB, and a characteristic absorption peak exists at 650 nm; meanwhile, the generated oxTMB can quench red fluorescence of RhB at HOF at 580 nm through an inner filtering effect; the color and fluorescence intensity change have a linear relationship with the concentration of the thiram, and the method can be used for quantitative detection of the thiram. Along with the increase of the concentration of thiram, the color of the sensing sponge is changed from pink to blue-green due to the generation of oxTMB under sunlight, and the red fluorescence is gradually weakened due to the fluorescence quenching effect under ultraviolet light. Therefore, dual-mode visual detection of colorimetric and fluorescence signals can be realized.

The invention relates to the technical field of surface enhanced Raman substrate preparation, in particular to a preparation method and application of an MXene (at) ZIF-8 (at) AuNPs composite SERS (Surface Enhanced Raman Scattering) substrate, and the preparation method comprises the following steps: constructing a photoelectric double-gain MXene (at) ZIF-8 heterojunction by adopting an in-situ solvothermal method, and loading AuNPs on MXene (at) ZIF-8 according to the action of an amido bond to form the MXene (at) ZIF-8 (at) AuNPs composite SERS substrate. The ZIF-8 can effectively inhibit the surface oxidation of the MXene material, so that the composite substrate has excellent stability. An electromagnetic field enhancement effect generated by an SERS'hot spot 'structure is cooperated with a charge transfer effect caused by MXene (at) ZIF-8, so that the detection sensitivity of the composite substrate is greatly improved. Moreover, the preparation method is simple and easy to implement, low in cost and suitable for large-scale preparation, and additional polyelectrolyte does not need to be introduced as a bridging agent. The MXene (at) ZIF-8 (at) AuNPs composite SERS substrate can be used for effectively detecting the pesticide residue thiram, and the detection limit can reach 5.0 * 10 <-12 > M.

The invention belongs to the technical field of pesticide residue detection, and relates to a molecularly imprinted carbon dot and a method for detecting pesticide thiram residue by using the molecularly imprinted carbon dot. The preparation method of the molecularly imprinted carbon dots comprises the following steps: (1) taking biomass and cysteine as raw materials, and preparing N, S co-doped carbon dots through microwave treatment and hydrothermal reaction; and (2) carrying out polymerization reaction on the N, S co-doped carbon dots and thiram under ultraviolet irradiation, and removing the thiram to obtain the molecularly imprinted carbon dots. By utilizing the molecularly imprinted carbon dots, the pesticide thiram residue in a sample can be detected in a high-specificity, low-false-positive-rate and high-accuracy manner.

The invention discloses a fluorescent microneedle sensor based on DNA-silver nanocluster hydrogel as well as a preparation method and application of the fluorescent microneedle sensor. According to the sensor, polyacrylamide hydrogel is used as a flexible matrix, and DNA-silver nanoclusters are uniformly embedded into the flexible matrix to serve as a fluorescent indicator. The preparation method comprises the following steps: mixing an acrylamide monomer, a cross-linking agent, a DNA-silver nano-cluster solution, an initiator and a catalyst, and carrying out polymerization and one-step molding in a microneedle mold under the initiation of ultraviolet light. The prepared sensor has the advantages of excellent flexibility, high stretch rate, surface adhesion and stable fluorescence property. When the fluorescent probe is used for detecting thiram, the low detection limit of 0.199 mu M and good linear response can be realized based on the specific fluorescence quenching effect of the fluorescent probe. The sensor can be used for on-site rapid screening of pesticide residues on the surfaces of foods such as fruits and seafood without complex instruments, and has a wide application prospect in the fields of food safety and environmental monitoring.

The invention belongs to the technical field of SERS (Surface Enhanced Raman Scattering) and food safety detection, and particularly relates to application of an Ag-GO-PAM (Polyacrylamide) flexible SERS substrate to rapid detection of pesticide residues on the surface of pericarp. According to the method, the flexible SERS substrate is used for wiping the surface of the bent peel and then is taken down, and Raman spectrum detection of the pesticide thiram is carried out. The substrate is used for detecting thiram, and has the advantages that by adding GO, PAM hydrogel can form a pore structure which is uniform in pore size and adapts to pesticide molecule adsorption and mass transfer, meanwhile, the coffee ring effect caused by excessive aggregation or uneven dispersion of silver nanoparticles is inhibited, the silver nanoparticles are promoted to form a uniform SERS hot spot area, and the detection sensitivity is improved. And collaborative optimization of SERS detection sensitivity and stability is realized. An oxygen-containing functional group on the surface of the GO and the thiram bimolecule form specific interaction, so that a Raman signal of the thiram bimolecule can be remarkably amplified; the substrate is easy and convenient to prepare, complex pretreatment is not needed, and a reliable technology is provided for efficient detection of pesticide residues.

The invention relates to the technical field of SERS (Surface Enhanced Raman Scattering) sensors, in particular to a preparation method and application of an MXene (at) ZIF-67 (at) AuNPs sensor, and the preparation method comprises the following steps: firstly, synthesizing an MXene (at) ZIF-67 heterojunction with a photoelectric double-gain characteristic through an in-situ solvothermal method; then gold nanoparticles (AuNPs) are loaded on the surface of the MXene (at) ZIF-67 heterojunction by means of an amide bonding effect, and the MXene (at) ZIF-67 (at) AuNPs composite SERS substrate is constructed; finally, an SERS analysis platform for pesticide thiram residue detection is constructed. The composite substrate fully utilizes a conductive network of MXene and a plasma resonance effect of AuNPs to synergistically enhance an electromagnetic field, and meanwhile, by virtue of an enrichment effect of a ZIF-67 porous structure on target molecules and a formed hot spot effect, the Raman signal intensity and the detection sensitivity of thiram are remarkably improved. Experimental results show that a detection method based on the substrate is simple and convenient to operate, can realize efficient detection of thiram, has a low detection limit of 1.0 * 10 <-11 > M, and is suitable for high-precision detection of trace thiram pesticide in a complex matrix.

The invention discloses a Balb / c mouse source hybridoma cell strain secreting an anti-thiram pesticide broad-spectrum monoclonal antibody and a gold nanoflower immunochromatography test strip for detecting thiram pesticides. The preservation number of the Balb / c mouse source hybridoma cell strain in the China General Microbiological Culture Collection Center is CGMCC (China General Microbiological Culture Collection Center) No.46545. The gold nanoflower immunochromatographic test strip for detecting the thiram pesticides is prepared by preparing a gold nanoflower immunoprobe from the thiram pesticide-resistant broad-spectrum monoclonal antibody secreted by the Balb / c mouse murine hybridoma cell strain and finally preparing the gold nanoflower immunochromatographic test strip for detecting the thiram pesticides. The gold nanoflower immunochromatography test strip is short in determination time, easy to operate and low in cost, has excellent specificity and sensitivity, and can simply, conveniently and quickly detect thiram pesticides.

The invention discloses a method for synchronously monitoring and degrading thiram in a water body based on an Au-TiO-plated optical fiber surface enhanced Raman scattering (SERS) sensor, and belongs to the technical field of application of an optical fiber sensing technology to photocatalysis. The invention provides an optical fiber SERS (Surface Enhanced Raman Scattering) sensing technical method integrating trace detection and photocatalytic degradation functions aiming at the problems of long detection period, incapability of in-situ monitoring, lack of synchronous degradation capability and the like in the existing pesticide residue detection method. The Au-TiO composite catalytic layer is constructed on the end face of the optical fiber, high-sensitivity Raman enhanced detection of thiram bimolecules is achieved, and target pollutants are synchronously degraded under ultraviolet irradiation by utilizing the photocatalytic performance of TiO. The device solves the problem that in-situ monitoring and rapid treatment of pesticide residues are difficult to realize in the prior art, has the advantages of being compact in structure, suitable for field application and the like, realizes integration of detection and treatment, and is suitable for rapid response and treatment of pesticide pollution in typical water environments such as agricultural drainage and drinking water sources.

The present application belongs to the technical field of pest control, and particularly relates to a kind of fungi of the order of Mucorales in the class of Sordariomycetes and its application. The fungi is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 42531. The fungi can be used for screening hazelnut disease-resistant lines or controlling hazelnut black spot disease. The present application proves by tests that eight kinds of pesticides, including penthiopyrad, mancozeb, difenconazole, pyraclostrobin, thiram, tridemorph, kresoxim-methyl and thiophanate-methyl, all have inhibitory effects on the fungi, which provides a new direction for controlling hazelnut black spot disease.

The present application provides a kind of fungicidal composition containing prothioconazole and thiram, comprising active ingredient and synergist, wherein active ingredient is composed of prothioconazole and thiram, and the mass ratio of prothioconazole and thiram is 1:5-5:1;Synergist is one or more of salicylic acid, oxalic acid or citric acid.The fungicidal composition of the present application has significant synergistic effect, and the addition of synergist (salicylic acid, oxalic acid, citric acid) in the composition can significantly improve the control effect of the fungicide on wheat scab and wheat powdery mildew, effectively reduce the dosage of active ingredient, slow down the development of drug resistance of pathogen, reduce environmental pollution and ensure food safety.