23 results about "Thiram" patented technology

The invention discloses a method for qualitatively and quantitatively analyzing and detecting the content of sodium dimethyl dithiocarbamate through high performance liquid chromatography. The method comprises the detection steps of preparation of a mobile phase, preparation of a standard substance solution, drawing of a calibration curve, detection of the content of a sample, qualitative analysis of the sample and standard recovery verification. Chromatographic conditions are as follows: a chromatographic column is a high-alkali-resistant reversed-phase chromatographic column, and the pH value application range is 1-12; the wavelength is 260 nm; the flow rate is 0.8 to 1 mL / min; the sample injection volume is 10-25 [mu] L; the column temperature is 30 DEG C; and the acquisition time is 25 minutes. The method has the beneficial effects that derivatization and earlier-stage treatment are not needed before detection and analysis of a sample. The mobile phase of the chromatographic instrument is alkaline and is used for dissolving and diluting a standard substance and a sample so as to keep the stability of the property of sodium dimethyl dithiocarbamate. The sodium dimethyl dithiocarbamate standard substance is dried to constant weight, crystal water and free water in the sodium dimethyl dithiocarbamate standard substance are removed on the premise of ensuring that the sodium dimethyl dithiocarbamate standard substance is not thermally decomposed, and the accuracy of calibration curve drawing and the accuracy of a sample calculation result are ensured. Qualitative analysis and standard recovery verification means are added, so that the accuracy of a detection result and the guiding significance of practical application are further improved.

The present invention is applicable to the technical field of pesticide residue detection, and provides a method for identifying the distribution of pesticide residues in plant tissues based on in-situ mass spectrometry imaging. Based on normal pressure mass spectrometry imaging, thiram is used as a non-systemic pesticide representative, and cymoxanil, imidacloprid, and pyraclostrobin are used as systemic pesticide representatives to establish a normal pressure mass spectrometry imaging in-situ detection method for non-systemic and systemic pesticides in fresh bananas. Non-systemic pesticide thiram does not enter the inside of the banana, and systemic pesticides cymoxanil, imidacloprid, and pyraclostrobin enter the inside of the banana in a lateral penetration and longitudinal migration manner over time; the smaller the water-octanol ratio distribution coefficient and the relative molecular mass of the pesticide, the faster the migration speed and the more content, and the migration speed and content are: cymoxanil>imidacloprid>pyraclostrobin; the faster the speed of pesticide migration in the more mature bananas, the more residual content, and it is not easy to digest. The present invention helps to guide the rational application of pesticides and provide a reference for pesticide safety evaluation.

The invention relates to a preparation method and application of an ordered silver nanoparticle and annular nano-gap array film, in particular to construction of a silver nanoparticle modified ordered annular nano-gap array film surface enhanced Raman scattering (SERS) substrate and application of the substrate to detection of environmental pollution wastewater. The method comprises the following steps: firstly, preparing a PAN nano pit array film by using an ordered silicon nanopillar array template based on a tape casting method, and then filling spherical particles such as silicon dioxide nanospheres or polystyrene spheres in nano pits by using a vertical sedimentation method so as to obtain an annular gap array film; sputtering silver nanoparticles on the surface of the annular gap array film by adopting an ion sputtering method, so as to obtain the ordered silver nanoparticle modified annular gap array film; due to the fact that the annular nanometer gap has a multi-coupling local electromagnetic field enhancement mode, the thin film shows an excellent SERS effect, low-concentration detection of organic pollutants such as rhodamine R6G, crystal violet and pesticide thiram can be achieved by using the thin film as an SERS substrate, and the thin film has excellent SERS sensitivity and signal uniformity and can be used for detecting organic pollutants such as rhodamine R6G, crystal violet and pesticide thiram. The method has a wide application prospect in the field of environmental pollutant detection.

The invention relates to the technical field of pesticides, and discloses a seed treatment agent and application thereof. The seed treatment agent comprises an active component and an auxiliary agent, wherein the active ingredients are prepared from flonicamid, thiram and azoxystrobin. The invention also relates to application of the seed treating agent in prevention and treatment of diseases and insect pests of food crops and oil crops. The invention provides the seed treatment agent containing the three active ingredients of flonicamid, thiram and azoxystrobin, the product stability is improved, the seed treatment agent can tolerate high and low temperature intervals, meanwhile, the product suspension rate is high, the coating falling rate is low, the seed treatment safety is good, and the seed treatment agent can be subsequently used in different countries and regions and is beneficial to large-scale popularization and application. The seed treatment agent provided by the invention is simple in preparation process and simple and convenient to operate.

The invention discloses a cyclodextrin metal organic framework composite flexible SERS (Surface Enhanced Raman Scattering) substrate for instantly detecting pesticide residues as well as preparation and application thereof, a cyclodextrin metal organic framework material (CD-MOF) is used as a carrier, gold nanoparticles are synthesized in the structure of the carrier, and a gold-loaded cyclodextrin metal organic framework material (Au at CD-MOF) is obtained; then, glycerol triglycidyl ether (GTE) is used as a cross-linking agent, and a cross-linked gold-loaded cyclodextrin metal organic framework material (CL-Au and CD-MOF) is synthesized; and finally, taking filter paper as a three-dimensional template, and combining with polydimethylsiloxane (PDMS) to prepare the Paper CL-Au CD-MOF / PDMS composite flexible substrate with relatively good SERS detection performance. The composite flexible substrate has good SERS detection performance, the detection limit of thiram on the surfaces of apples is 4.2 * 10 <-8 > M, and the detection limit of thiram on the surfaces of cucumbers is 1 ppm.

The invention relates to a complex microbial inoculant for preventing and treating tobacco bacterial wilt and application of the complex microbial inoculant. Strain in the complex microbial inoculant comprises a mulberry enterobacter 65D6 strain, a Ralstonia 56D2 strain, an agrobacterium 18A6 strain, an arthrobacter uretogenes 05-507 strain and a bacillus safensis 05-2101 strain. The five specific strains are compounded and combined for use, an excellent tobacco bacterial wilt prevention and treatment effect is achieved, and experimental research shows that the disease occurrence of tobacco plants treated by the complex microbial inoculant is obviously slowed down, and the disease index is obviously lower than that of a clear water control group and that of a thiediazole copper and chloriure.copper sulfate chemical agent control group. Meanwhile, the five strains can be mutually matched and promoted, a synergistic effect is achieved in the aspect of preventing and treating tobacco bacterial wilt, the WY11 strain is further added for combined use, the effect of preventing and treating the tobacco bacterial wilt is improved, and the better effect of promoting the growth of tobacco plants can be achieved.

The invention provides a functionalized melamine sensing sponge, a preparation method thereof and a thiram colorimetric-fluorescence visual dual-mode detection method, and belongs to the technical field of food safety detection. According to the invention, thiram is specifically combined with PDA-coated CuO2 in the sensing sponge, so that the nano-enzyme catalytic activity of the sensing sponge is excited, colorless TMB is catalyzed to be oxidized into blue oxTMB, and a characteristic absorption peak exists at 650 nm; meanwhile, the generated oxTMB can quench red fluorescence of RhB at HOF at 580 nm through an inner filtering effect; the color and fluorescence intensity change have a linear relationship with the concentration of the thiram, and the method can be used for quantitative detection of the thiram. Along with the increase of the concentration of thiram, the color of the sensing sponge is changed from pink to blue-green due to the generation of oxTMB under sunlight, and the red fluorescence is gradually weakened due to the fluorescence quenching effect under ultraviolet light. Therefore, dual-mode visual detection of colorimetric and fluorescence signals can be realized.

The invention relates to the technical field of surface enhanced Raman substrate preparation, in particular to a preparation method and application of an MXene (at) ZIF-8 (at) AuNPs composite SERS (Surface Enhanced Raman Scattering) substrate, and the preparation method comprises the following steps: constructing a photoelectric double-gain MXene (at) ZIF-8 heterojunction by adopting an in-situ solvothermal method, and loading AuNPs on MXene (at) ZIF-8 according to the action of an amido bond to form the MXene (at) ZIF-8 (at) AuNPs composite SERS substrate. The ZIF-8 can effectively inhibit the surface oxidation of the MXene material, so that the composite substrate has excellent stability. An electromagnetic field enhancement effect generated by an SERS'hot spot 'structure is cooperated with a charge transfer effect caused by MXene (at) ZIF-8, so that the detection sensitivity of the composite substrate is greatly improved. Moreover, the preparation method is simple and easy to implement, low in cost and suitable for large-scale preparation, and additional polyelectrolyte does not need to be introduced as a bridging agent. The MXene (at) ZIF-8 (at) AuNPs composite SERS substrate can be used for effectively detecting the pesticide residue thiram, and the detection limit can reach 5.0 * 10 <-12 > M.

The invention belongs to the technical field of wastewater treatment, and particularly relates to a deep recovery process of thiram production wastewater. The preparation method comprises the following specific steps: dissolving potassium persulfate and aluminum sulfate in water to prepare an initiator for later use; sequentially adding 0.5-5 volume of hydrogen peroxide and 0.5-5 volume of the initiator into the reaction equipment, uniformly mixing, and adjusting the pH value of the system to 1-7; adding 50-150 volume of the thiram production wastewater to be treated into the reaction equipment, stirring, and controlling the temperature of the system to be 10-50 DEG C; and after the reaction is finished, recovering the temperature of the system to room temperature, and centrifuging and drying to obtain thiram and treated wastewater. According to the method disclosed by the invention, the thiram active compound is obtained from the thiram production wastewater, the COD (Chemical Oxygen Demand) content and the ammonia nitrogen content in the wastewater are simultaneously reduced, the COD of the wastewater can be reduced to 5000mg / L or below, the reduction amplitude of the ammonia nitrogen content is close to 93% at most, and the subsequent treatment difficulty is reduced.

The invention belongs to the technical field of pesticide residue detection, and relates to a molecularly imprinted carbon dot and a method for detecting pesticide thiram residue by using the molecularly imprinted carbon dot. The preparation method of the molecularly imprinted carbon dots comprises the following steps: (1) taking biomass and cysteine as raw materials, and preparing N, S co-doped carbon dots through microwave treatment and hydrothermal reaction; and (2) carrying out polymerization reaction on the N, S co-doped carbon dots and thiram under ultraviolet irradiation, and removing the thiram to obtain the molecularly imprinted carbon dots. By utilizing the molecularly imprinted carbon dots, the pesticide thiram residue in a sample can be detected in a high-specificity, low-false-positive-rate and high-accuracy manner.

The invention discloses a fluorescent microneedle sensor based on DNA-silver nanocluster hydrogel as well as a preparation method and application of the fluorescent microneedle sensor. According to the sensor, polyacrylamide hydrogel is used as a flexible matrix, and DNA-silver nanoclusters are uniformly embedded into the flexible matrix to serve as a fluorescent indicator. The preparation method comprises the following steps: mixing an acrylamide monomer, a cross-linking agent, a DNA-silver nano-cluster solution, an initiator and a catalyst, and carrying out polymerization and one-step molding in a microneedle mold under the initiation of ultraviolet light. The prepared sensor has the advantages of excellent flexibility, high stretch rate, surface adhesion and stable fluorescence property. When the fluorescent probe is used for detecting thiram, the low detection limit of 0.199 mu M and good linear response can be realized based on the specific fluorescence quenching effect of the fluorescent probe. The sensor can be used for on-site rapid screening of pesticide residues on the surfaces of foods such as fruits and seafood without complex instruments, and has a wide application prospect in the fields of food safety and environmental monitoring.

The invention belongs to the technical field of SERS (Surface Enhanced Raman Scattering) and food safety detection, and particularly relates to application of an Ag-GO-PAM (Polyacrylamide) flexible SERS substrate to rapid detection of pesticide residues on the surface of pericarp. According to the method, the flexible SERS substrate is used for wiping the surface of the bent peel and then is taken down, and Raman spectrum detection of the pesticide thiram is carried out. The substrate is used for detecting thiram, and has the advantages that by adding GO, PAM hydrogel can form a pore structure which is uniform in pore size and adapts to pesticide molecule adsorption and mass transfer, meanwhile, the coffee ring effect caused by excessive aggregation or uneven dispersion of silver nanoparticles is inhibited, the silver nanoparticles are promoted to form a uniform SERS hot spot area, and the detection sensitivity is improved. And collaborative optimization of SERS detection sensitivity and stability is realized. An oxygen-containing functional group on the surface of the GO and the thiram bimolecule form specific interaction, so that a Raman signal of the thiram bimolecule can be remarkably amplified; the substrate is easy and convenient to prepare, complex pretreatment is not needed, and a reliable technology is provided for efficient detection of pesticide residues.

The invention belongs to the field of pesticides, and relates to a pesticide insecticidal and bactericidal composition containing metafluzamide and tetrachlorantraniliprole and application thereof. According to the composition, a component A, a component B and a component C are mixed to serve as an active component, the component A is selected from metafluzamide, the component B is selected from tetrachlorantraniliprole, and the component C is selected from one or more of carbendazim, metalaxyl-M, fludioxonil, difenoconazole, azoxystrobin, prochloraz, tebuconazole and thiram; the weight part ratio of the component A to the component B to the component C is (1-20): (1-20): (1-20). The composition provided by the invention can improve the control effect and reduce the dosage, has good insecticidal and bactericidal effects, and also comprises a preparation, a concentrate and a seed treatment agent containing the composition.

The invention discloses a controlled-release and multi-effect cabbage carbon-based biogas manure and a preparation method thereof. The cabbage carbon-based biogas manure is prepared from the following components: biomass charcoal, biogas slurry, a coated slow-release coating, a compound chemical fertilizer, indolebutyric acid, naphthylacetic acid, quintozene, thiram and bentonite. The biomass charcoal, the biogas slurry, the composite chemical fertilizer and the bentonite are mixed and granulated to form particles, and the particles are sequentially coated with a coated slow-release coating, an indolebutyric acid and naphthylacetic acid mixing layer, a coated slow-release coating, a quintozene and thiram mixing layer and a coated slow-release coating. According to the present invention, the effects of soil disinfection and sterilization can be achieved, crop rooting can be promoted, the crop survival rate can be improved, cabbage soil-borne diseases can be prevented and treated, the problem of single nutrient and single effect of the traditional carbon-based biogas fertilizer can be avoided, and the multiple effects of the fertilizer can be achieved. Meanwhile, slow-release fertilization is realized by controlling the coating thickness.

The invention discloses a corn disease and pest control agent and a preparation method and application thereof. The agent is prepared from the following raw materials in parts by weight: 40 to 60 parts of walnut peel extract, 30 to 40 parts of melia azedarach extract, 30 to 40 parts of pyrethrum extract, 20 to 30 parts of carbendazim, 10 to 20 parts of fludioxonil, 10 to 20 parts of thiram and 2 to 5 parts of astragalus polysaccharide. The preparation method specifically comprises the following steps: (1) weighing the raw materials; and (2) uniformly mixing the raw materials to obtain the product. The pesticide is reasonable in component matching and complementary in pesticide effect, the pesticide effects of the components are fully exerted, the pesticide has a remarkable synergistic effect, has a remarkable prevention and treatment effect on common corn diseases and insect pests, can greatly reduce the occurrence rate of the corn diseases and insect pests, so that the yield and quality of corn are greatly improved, and the pesticide is broad in spectrum, efficient, low in toxicity, free of chemical pollution and worthy of popularization. And the corn plants cannot generate drug resistance.

The invention relates to the technical field of SERS (Surface Enhanced Raman Scattering) sensors, in particular to a preparation method and application of an MXene (at) ZIF-67 (at) AuNPs sensor, and the preparation method comprises the following steps: firstly, synthesizing an MXene (at) ZIF-67 heterojunction with a photoelectric double-gain characteristic through an in-situ solvothermal method; then gold nanoparticles (AuNPs) are loaded on the surface of the MXene (at) ZIF-67 heterojunction by means of an amide bonding effect, and the MXene (at) ZIF-67 (at) AuNPs composite SERS substrate is constructed; finally, an SERS analysis platform for pesticide thiram residue detection is constructed. The composite substrate fully utilizes a conductive network of MXene and a plasma resonance effect of AuNPs to synergistically enhance an electromagnetic field, and meanwhile, by virtue of an enrichment effect of a ZIF-67 porous structure on target molecules and a formed hot spot effect, the Raman signal intensity and the detection sensitivity of thiram are remarkably improved. Experimental results show that a detection method based on the substrate is simple and convenient to operate, can realize efficient detection of thiram, has a low detection limit of 1.0 * 10 <-11 > M, and is suitable for high-precision detection of trace thiram pesticide in a complex matrix.

The invention discloses a colorimetric hydrogel reactor based on iron-based nano horseradish peroxidase, and provides a sensor capable of detecting thiram residues on site and a preparation method. The iron-based nano-enzyme with natural horse radish peroxidase activity is synthesized by taking ferrous chloride and glutathione as materials through a hydrothermal method. The iron-based nano-enzyme shows huge potential in the aspect of detection and application of pesticide thiram, and when thiram exists, active sites of the iron-based nano-enzyme are passivated, the catalytic activity is reduced, and the output color is changed. The construction of a hydrogel colorimetric platform based on a nano material simulating horseradish peroxidase can realize rapid visual and quantitative detection of thiram residues on site, and can promote the development of nano enzyme-based sensors and on-site monitoring application. The method has the advantages of being convenient to operate, economical and effective in mechanism, high in stability and the like, has excellent on-site practicability, and provides a new ideal platform for on-site monitoring of precision agriculture and food safety risk assessment.

The invention discloses a Balb / c mouse source hybridoma cell strain secreting an anti-thiram pesticide broad-spectrum monoclonal antibody and a gold nanoflower immunochromatography test strip for detecting thiram pesticides. The preservation number of the Balb / c mouse source hybridoma cell strain in the China General Microbiological Culture Collection Center is CGMCC (China General Microbiological Culture Collection Center) No.46545. The gold nanoflower immunochromatographic test strip for detecting the thiram pesticides is prepared by preparing a gold nanoflower immunoprobe from the thiram pesticide-resistant broad-spectrum monoclonal antibody secreted by the Balb / c mouse murine hybridoma cell strain and finally preparing the gold nanoflower immunochromatographic test strip for detecting the thiram pesticides. The gold nanoflower immunochromatography test strip is short in determination time, easy to operate and low in cost, has excellent specificity and sensitivity, and can simply, conveniently and quickly detect thiram pesticides.

The invention discloses a method for synchronously monitoring and degrading thiram in a water body based on an Au-TiO-plated optical fiber surface enhanced Raman scattering (SERS) sensor, and belongs to the technical field of application of an optical fiber sensing technology to photocatalysis. The invention provides an optical fiber SERS (Surface Enhanced Raman Scattering) sensing technical method integrating trace detection and photocatalytic degradation functions aiming at the problems of long detection period, incapability of in-situ monitoring, lack of synchronous degradation capability and the like in the existing pesticide residue detection method. The Au-TiO composite catalytic layer is constructed on the end face of the optical fiber, high-sensitivity Raman enhanced detection of thiram bimolecules is achieved, and target pollutants are synchronously degraded under ultraviolet irradiation by utilizing the photocatalytic performance of TiO. The device solves the problem that in-situ monitoring and rapid treatment of pesticide residues are difficult to realize in the prior art, has the advantages of being compact in structure, suitable for field application and the like, realizes integration of detection and treatment, and is suitable for rapid response and treatment of pesticide pollution in typical water environments such as agricultural drainage and drinking water sources.

The invention belongs to the technical field of pathogen biology, and discloses a method for determining biological characteristics of amorphophallus bulbifer leaf spot bacteria and jointly screening endophytic bacteria-agents, and the research on the biological characteristics of pathogen alternaria tenuissima finds that the bacteria grow most favorably under a full-illumination condition, the optimal growth temperature is 25-28 DEG C, and the optimal pH value is 9; the strain has the best utilization effect on a nitrogen source peptone and a carbon source D-fructose, and the relatively optimal culture medium is a CAS culture medium. In the 10 screened endophytic antagonistic bacteria, the bacterial strain J4 (B. velezensis) shows the strongest antibacterial effect on the pathogenic bacteria. In addition, the indoor bacteriostasis test results of seven different medicaments for test on the pathogenic bacteria show that the inhibition effect of 50% thiram WP on the pathogenic bacteria is most significant.

The present invention discloses a preparation method for a three-dimensional SERS substrate for detecting thiram, comprising: growing zinc oxide nanorods on a polyvinylidene fluoride membrane to obtain a ZnO / P substrate; coating a gold film on the surface of the zinc oxide nanorods to obtain an Au / ZnO / P substrate; modifying the Au / ZnO / P substrate and adsorbing silver nanoparticles on the surface of the modified Au / ZnO / P substrate to obtain an Ag / Au / ZnO / P substrate, which is the three-dimensional SERS substrate. The present invention also provides the substrate obtained by the above preparation method. The present invention introduces bimetallic nanoparticles as enhancement materials, synergistically combines the respective advantages of Ag and Au, and utilizes the three-dimensional morphology of ZnO nanorods to generate rich SERS "hot spots" and enhancement effects. At the same time, due to the special heterointerface structure of the bimetallic material, a stronger coupling effect can be generated with thiram, realizing rapid in-situ high-sensitivity detection of thiram.

The present application belongs to the technical field of pest control, and particularly relates to a kind of fungi of the order of Mucorales in the class of Sordariomycetes and its application. The fungi is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 42531. The fungi can be used for screening hazelnut disease-resistant lines or controlling hazelnut black spot disease. The present application proves by tests that eight kinds of pesticides, including penthiopyrad, mancozeb, difenconazole, pyraclostrobin, thiram, tridemorph, kresoxim-methyl and thiophanate-methyl, all have inhibitory effects on the fungi, which provides a new direction for controlling hazelnut black spot disease.

The present application provides a kind of fungicidal composition containing prothioconazole and thiram, comprising active ingredient and synergist, wherein active ingredient is composed of prothioconazole and thiram, and the mass ratio of prothioconazole and thiram is 1:5-5:1;Synergist is one or more of salicylic acid, oxalic acid or citric acid.The fungicidal composition of the present application has significant synergistic effect, and the addition of synergist (salicylic acid, oxalic acid, citric acid) in the composition can significantly improve the control effect of the fungicide on wheat scab and wheat powdery mildew, effectively reduce the dosage of active ingredient, slow down the development of drug resistance of pathogen, reduce environmental pollution and ensure food safety.