35 results about "Alginate hydrogel" patented technology

The invention discloses an alginic acid hydrogel micro-robot pH response deformation dynamic modeling method, system and device based on a convolutional neural network, a medium and a product, and relates to the technical field of micro-robot deformation dynamic modeling. The method comprises the following steps: collecting a time sequence image and a pH sequence of micro-robot deformation, and tracking feature points to extract time sequence displacement data; preprocessing the collected data, including cleaning, anomaly detection, sequence alignment and standardization; constructing a one-dimensional convolutional neural network model, and predicting the time sequence displacement data by taking the pH sequence as input; and training and optimizing the model by using preprocessed data, and determining network structure parameters by adopting hyper-parameter optimization. According to the method, accurate dynamic modeling and prediction of the pH response deformation of the alginic acid hydrogel micro-robot are realized, and the behavior control precision of the alginic acid hydrogel micro-robot in a complex environment is improved.

The present invention discloses a selenium-loaded photocross-linked alginate hydrogel and a preparation method thereof, and relates to the technical field of polymer gels. The preparation method is as follows: first, alginate is dissolved in deionized water, sodium chloride and a buffer are added and stirred until completely dissolved, the pH is adjusted to weak acidity, N-hydroxy-sulfosuccinimide and a condensing agent are added, and after stirring evenly, 2-aminoethyl methacrylate hydrochloride is added to carry out a methacrylate reaction to obtain methacrylated alginate. The alginate and a photoinitiator are separately dissolved in deionized water to prepare a storage solution, and selenomethionine is dissolved in the photoinitiator storage solution. Finally, a photoinitiator solution containing selenomethionine and a methacrylated alginate storage solution are mixed, and a photocross-linking reaction is initiated by ultraviolet irradiation to obtain a selenium-loaded photocross-linked alginate hydrogel. The present invention introduces selenomethionine into a photocross-linked alginate hydrogel scaffold, which has good cell biocompatibility and promotes osteogenesis.

The invention discloses an all-weather high-performance fiber supercapacitor based on graphene oxide / metal organic framework co-doped alginate hydrogel electrolyte (GNS) and a preparation method and application thereof. The all-weather high-performance fiber supercapacitor comprises two MXene electrodes and a gel electrolyte, wherein the two MXene electrodes are arranged in parallel; the gel electrolyte is filled between the two MXene electrodes and wraps the two MXene electrodes; the gel electrolyte comprises sodium alginate gel, and graphene oxide and a metal organic framework material which are dispersed in the sodium alginate gel. The graphene oxide and the metal organic framework material are introduced into the gel electrolyte, so that the transmission rate and the ionic conductivity of ions in the gel electrolyte are improved; and a large number of metal ions exist in the hydrogel electrolyte, so that the hydrogel electrolyte still keeps good electrical properties in a low-temperature environment. The capacitor provided by the invention has wide temperature adaptability, high ionic conductivity and excellent charge storage capability.

The invention discloses an intelligent alginate-chitosan oligosaccharide microsphere which is prepared by the following method: (1) adding functional protein into a sodium alginate solution; (2) dropwise adding calcium salt or a solution thereof, and inducing ionic crosslinking to obtain alginate hydrogel; (3) adding 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide, and fully activating the carboxyl group on the chain of the alginate; and (4) dropwise adding a chitosan oligosaccharide solution, so that amino groups of chitosan oligosaccharide and the activated carboxyl groups are subjected to covalent coupling, and the intelligent alginate-chitosan oligosaccharide microspheres are obtained. The invention further discloses application of the intelligent alginate-chitosan oligosaccharide microspheres in serving as or preparing an intestinal tract positioning release preparation and application in delivering functional protein. The intelligent alginate-chitosan oligosaccharide microspheres can significantly prolong the residence time of encapsulated protein in intestinal tracts, show excellent cytocompatibility and in-vivo biological safety, and provide safety guarantee for oral application of the microspheres.

The present invention is directed to a composition including a plurality of capsules, wherein each capsule of the plurality of capsules includes polyvinyl alcohol (PVA), polyvinyl pyrrolidone (PVP), alginate, and at least one cell. Further provided are a method of preparing the composition of the invention, as well as using thereof, such as for feeding a subject.

The application provides a multifunctional microalgae-based hydrogel wound dressing and a preparation method and application thereof, and belongs to the field of hydrogel dressings. Microalgae are embedded in a hydrogel network formed by modified gelatin and cross-linked sodium alginate, and a multifunctional alginate hydrogel is constructed for chronic wound repair of diabetes. The selected microalgae can realize in-situ synthesis and uniform distribution of small-sized gold nanoparticles on the surface thereof during co-culture with high-concentration gold ions, stimulate high-efficiency secretion of extracellular polysaccharides by the microalgae, and maintain high cell activity. The prepared dressing is helpful for relieving a local hypoxic microenvironment of a wound and promoting vascular regeneration, can effectively remove excess active oxygen at a wound site, and can induce macrophages to polarize to M2 type; in addition, the gold nanoparticles in the hydrogel network have good antibacterial performance.

The invention discloses a preparation method of hydrogel for improving a diabetic chronic wound microenvironment, and relates to the technical field of biomedical materials. Comprising the following steps: dissolving sodium alginate and gallic acid in water to form a mixed solution, and adding a calcium ion solution for crosslinking to prepare antioxidant alginate hydrogel; the dosage of the sodium alginate is 100mg / 5mL of water, and the dosage of the gallic acid is 50mg / 5mL of water; the calcium ion solution is a CaCl2 solution with the concentration of 10 mg / mL, and the volume ratio of the added calcium ion solution to the mixed liquid is 1: 1. The multifunctional hydrogel disclosed by the invention realizes comprehensive treatment on chronic diabetic wounds through a synergistic effect mechanism; compared with traditional dressings, the hydrogel can synchronously solve the two core problems of excessive oxidative stress and biological membrane infection, and the wound microenvironment is fundamentally improved.

The invention relates to hydrogel microparticles, compositions and uses thereof. The present invention provides spherical or elliptical non-alginate hydrogel microparticles for local delivery and sustained release of therapeutic cells and / or tissues at an implantation site, the microparticles comprising a 3-dimensional substrate of a covalently crosslinked non-alginate polymer compound and a therapeutically effective amount of cells and / or tissues embedded therein, wherein the cells have a viability of at least 50%, the microparticles have a size of greater than 30 [mu] m, the non-alginate hydrogel microparticles are formed without using an oil emulsion, the polymer compound is polyethylene glycol vinyl sulfone or acrylated hyaluronic acid, the matrix further includes a cross-linking agent selected from the group consisting of dithiothreitol, polyethylene glycol dithiol, and ethylene glycol bis-mercaptoacetate, cross-linked with the non-alginate polymer compound. Compositions comprising such hydrogel microparticles and their use for the preparation of medicaments are also described.

The invention discloses a phosphate radical-based multi-metal oxide radiation protection paste as well as a preparation method and application thereof. The radiation protection paste is a shear thinning paste formed by uniformly dispersing a nano-scale multi-metal phosphate radiation protection filler in a cross-linked polymer hydrogel matrix, the nano-scale multi-metal phosphate anti-radiation filler is nano-scale multi-metal phosphate prepared from phosphate ions, bismuth ions, barium ions and zirconium ions through an in-situ coordination precipitation method, and the molar ratio of the bismuth ions to the barium ions to the zirconium ions is (3-4): (1.5-2.5): 1. The mass of the nanoscale multi-metal phosphate accounts for 35%-65% of the total mass of the multi-metal oxide radiation protection paste; the cross-linked polymer hydrogel matrix is calcium ion cross-linked alginate hydrogel. The radiation protection paste has the remarkable advantages of being convenient to construct, free of lead or low in toxicity, high in adaptability to complex structures and the like, and an efficient, flexible and environment-friendly new scheme is provided for radiation protection.

The application discloses a kind of hierarchical pH response telopeptide oral microcapsules and preparation method, belong to biomedicine technical field.A kind of hierarchical pH response microcapsule, comprising: inner phase core, including drug-loaded inorganic nanoparticles and first alginate material;And, outer shell, including the second alginate material wrapped in the outside of the inner phase core;Wherein the drug-loaded inorganic nanoparticles are the complex of polypeptide drug and layered silicate material;The polypeptide drug is telopeptide;The layered silicate material is montmorillonite.Compared with prior art, the pH response microcapsule of the application has good pH response and intestinal targeting release capacity;In intestinal environment, through special ion exchange and pH change, not only can promote the rapid degradation of alginate hydrogel shell, but also can effectively trigger the release of telopeptide from montmorillonite carrier, so as to realize the efficient release and absorption of target site.

A biologically active sorptive media is disclosed for sustainable contaminant removal in, for example, stormwater management systems. This technology addresses the technical problem of hydrophilic trace organic contaminants and dissolved-phase nutrients passing through conventional stormwater infrastructure, posing risks to groundwater and surface water quality. The biologically active sorptive media comprises a biopolymer matrix formed from alginate hydrogel, encapsulating sorbent materials (e.g., powdered activated carbon (PAC), iron-based water treatment residuals (FeWTR]), growth substrates (e.g., wood flour), and biodegrading organisms (e.g., white rot fungi). These beads enable rapid sorption of contaminants during storm events and subsequent biodegradation during inter-storm periods, renewing sorption capacity. The beads are mechanically robust, scalable, and stable for extended periods in high ionic strength solutions. Applications include bioaugmentation of green stormwater infrastructure (GSI), wastewater treatment, and bioremediation. This approach transforms GSI science by coupling sorption and biodegradation for enhanced contaminant removal.

The invention discloses a calcium-nickel-loaded cross-linked alginate microsphere, a pH regulation type embolism agent for continuously delivering IL-2 and application of the pH regulation type embolism agent, and nickel alginate hydrogel microspheres loaded with calcium carbonate are prepared and can be used as a novel embolism agent to enhance TAE immunotherapy. The CaNPs / Ni-ALGMS can realize the continuous release of IL-2, so that the risk of cytokine storm is obviously reduced. Due to the fact that CaCO3 is contained, the CaNPs / Ni-ALGMS can neutralize acidic TME, and the capacity of IL-2 for promoting immune cell infiltration can be remarkably enhanced. Therefore, the IL-2 loaded CaNPs / Ni-ALGMS (IL-2 at-CaNPs / Ni-ALGMS) can effectively inhibit the growth of primary and distant tumors, especially when the IL-2 loaded CaNPs / Ni-ALGMS is used in combination with immune checkpoint inhibition (ICB) treatment.

The invention provides a microcarrier for oral delivery of probiotic microcolonies as well as a preparation method and application of the microcarrier, and belongs to the technical field of probiotic delivery. According to the invention, an Express Microflora Service (EMS) system with adjustable matrix rigidity and viscoelasticity characteristics is designed to support culture and delivery of probiotic microcolonies based on a multicellular self-organization strategy, and the EMS system combines a free radical polymerization covalent cross-linked network with an ionic cross-linked network of classic calcium alginate, so as to support the culture and delivery of probiotic microcolonies based on the multicellular self-organization strategy. A series of double-crosslinked alginate hydrogel networks with stress relaxation characteristics are successfully developed, an optimized hydrogel formula for microcolony culture and oral administration is obtained, and a self-organizing mode of microcolonies formed by probiotics in a microcarrier is explored. The application of the EMS microcarrier in intestinal colonization shows that the probiotic microcolonies in the EMS microcarrier have excellent capability of resisting external severe environment and strong colonization potential, and the colitis of mice can be obviously relieved by orally taking EMS for one time.

This invention provides a high-strength alginate hydrogel, its preparation method, and its application, relating to the field of hydrogel materials technology. Using alginate as a raw material, this invention prepares a high-strength alginate hydrogel through chemical and ionic crosslinking. This solves the problem that conventional alginate hydrogels are prone to mechanical damage during use due to their low mechanical strength, and their performance significantly deteriorates after damage. Furthermore, the alginate hydrogel preparation method used in this invention features readily available raw materials, simple operation, and stable process and product quality, making it suitable for the industrial production of alginate hydrogels.

This disclosure discloses a method for preparing an alginate-based hydrogel colorimetric label. The method includes: adding alginate and a regulating material in a predetermined ratio to an aqueous solution containing an indicator solution and glycerol, stirring and heating to obtain a mixed solution; preparing an alginate hydrogel film from the mixed solution using a casting or casting method; uniformly spraying or coating a metal ion crosslinking agent onto the surface of the alginate hydrogel film for crosslinking treatment, and after standing, obtaining a crosslinked alginate hydrogel film; immersing the crosslinked alginate hydrogel film in an ethanol solution, and then inducing solvent exchange through ultrasound to obtain the alginate-based hydrogel colorimetric label. This application can improve the elastic modulus and colorimetric resolution of the colorimetric label.

Non-alginate hydrogel microparticles for localized delivery and sustained release of therapeutic cells and / or tissues (including homogenous or heterogenous cell clusters) at a site of implantation. The microparticles comprise a 3-dimensional matrix of covalently crosslinked non-alginate polymer compounds and a therapeutically-effective amount of cells and / or tissue entrapped therein, wherein the cells have a viability of at least about 50%, and wherein the microparticle has a size of greater than about 30 pm. Compositions containing such microparticles and methods of using such microparticles for treatment are also described.

The present invention refers to a method for growing and harvesting mammal adhesive cells by contacting them with alginate hydrogel particles having a cell-attachment substituent allowing the cells to adhere to the alginate particles and to be gently harvested, to a method for preparing such alginate particles, to a new type of alginate hydrogel particles and to a method for re-hydrating said alginate dry particles.

The application provides a preparation method of an anti-infection bone repair promoting preparation for sequentially regulating a macrophage immune microenvironment, which comprises the following steps: uniformly mixing 1-10% flavone modified hydroxyapatite, 2-10% sodium alginate hydrogel, 0.1-1% hydrogen sulfide sustained release carrier and 79-96.9% tricalcium phosphate in proportion by weight percentage, adding a calcium chloride aqueous solution, uniformly stirring, and freeze-drying. The flavone tricalcium phosphate, the calcium phosphate cement of the light crosslinking alginate hydrogel containing the hydrogen sulfide sustained release carrier, exhibits the function of regulating the macrophage immune microenvironment, and the preparation itself can better realize the function of sequentially inducing the macrophages in a bone damage microenvironment to be induced to M1 type polarization and then to M2 type polarization.

Described herein are exemplary embodiments and uses of implantable therapeutic and / or prophylactic agent delivery devices that may be comprised of one or more layers of collagen-alginate hydrogel.

The invention provides a preparation method of pH-responsive injectable algal polysaccharide hydrogel for drug delivery based on dynamic covalent bonds and application of the pH-responsive injectable algal polysaccharide hydrogel in chronic wounds, especially diabetic ulcer wounds. The preparation method of the dressing is simple and easy to implement, the prepared hydrogel has good water absorption and swelling rate, the hydrogel has pH responsiveness, intelligent drug release of the hydrogel according to the pH change of the wound microenvironment is facilitated, tissue regeneration is effectively promoted, and infection is inhibited. The hydrogel dressing not only has the characteristics of good air permeability, good hygroscopicity and the like, but also has the capability of inhibiting the growth of bacteria, and the hydrogel has injectability and can fill irregular wound areas and promote in-situ tissue regeneration so as to finally promote the healing of chronic wounds. The traditional Chinese medicine composition is especially suitable for clinically widely existing chronic difficult-to-heal wounds, especially for treatment and nursing of diabetic ulcers.

Disclosed are alginate-specific deoxyribonucleic acid (DNA) aptamers that specifically bind to alginate hydrogels and alginate hydrogels containing alginate-specific DNA aptamers. The alginate-specific DNA aptamers display slower diffusion out of alginate hydrogels and function as anchors for other biomolecules in a wide range of applications.

The application relates to the technical field of biomedical engineering, and discloses a sodium alginate hydrogel loaded with copper arsenite nanoparticles as well as a preparation method and application thereof. The hydrogel comprises a sodium alginate hydrogel matrix and copper arsenite nanoparticles loaded therein, the copper arsenite nanoparticles comprise Cu2+, arsenite ions and PLGA-PEG-NH2, the sodium alginate hydrogel loaded with the copper arsenite nanoparticles is used for in-situ solidification to form a drug storage after intratumoral injection. The application constructs a nanoparticle self-assembly system based on PLGA-PEG-NH2, uniformly loads a complex formed by arsenite ions and copper ions in the PLGA-PEG-NH2 carrier material, forms copper arsenite nanoparticles with uniform and controllable particle sizes through a self-assembly process, the nanoparticles form stable complexes through coordination complexation between Cu2+ and As3+, and have the characteristics of specific dissociation in a tumor acid microenvironment.

A method is provided for promoting salivary gland regeneration in a subject in need thereof comprising administering to acinar progenitor cells of the salivary gland at least one of a cholinergic agonist or muscarinic agonist to promote acinar cell generation. In particular, formulations comprising a muscarinic agonist such as cevimeline encapsulated in an alginate hydrogel can be formulated for local administration to a salivary gland and used in treatment of xerostomia.

The invention discloses a preparation method and application of photo-thermal biomass alginate hydrogel. The preparation method comprises the following steps: dissolving a photo-thermal conversion material in a natural biomass alginate solution, and uniformly dispersing; adding a biomass foaming agent into the obtained alginate solution, and stirring at room temperature to obtain mixed sol; the obtained mixed sol is poured into a mold for freeze drying, and aerogel is obtained; and carrying out synergistic crosslinking on the obtained aerogel through metal ion solutions with different valence states. The preparation method disclosed by the invention is simple in process and easy for large-scale production, and the obtained natural biomass alginate hydrogel has excellent photo-thermal performance.

This application discloses an experimental apparatus for the degradation of alginate hydrogel, belonging to the technical field of degradation testing devices. It includes, from top to bottom, an infusion device, a distributor, several sample chambers, and several body fluid recovery tanks. A first pipe connects the infusion device to the main channel of the distributor, and a check valve is installed on the first pipe. Each branch channel of the distributor corresponds to one of the sample chambers. A second pipe connects each branch channel of the distributor to its corresponding sample chamber, and a flow rate regulator is installed on the second pipe. Each sample chamber corresponds to one of the body fluid recovery tanks, and a third pipe connects each sample chamber to its corresponding body fluid recovery tank. This application improves experimental efficiency.

Described herein are hydrogel capsules (e.g., alginate hydrogel capsules) comprising polysaccharide polymers capable of covalent crosslinking to another moiety, such as another polysaccharide polymer; as well as related compositions and uses thereof.

The application discloses a microfluidic chip and a method for detecting water quality by embedding coliform group in alginate hydrogel, and the microfluidic chip comprises a sample inlet, an oil phase inlet, an alginate aqueous solution inlet, a droplet mixing flow channel, an incubation chamber and an outlet; wherein the oil phase inlet is connected with the sample flow channel through two oil phase flow channels which are divided into two parts and meet at a cross-shaped port, the alginate aqueous solution inlet is connected with the flow channel from the cross-shaped port through a water phase flow channel and meets at a T-shaped port; a contraction section flow channel and a first serpentine flow channel are sequentially arranged between the T-shaped port and the droplet mixing flow channel, and are respectively used for extruding and fusing the bacterial liquid droplet and the alginate droplet. The application solves the problem that the detection effect is poor due to the spontaneous fusion of droplets caused by the increase of incubation temperature in the prior art by combining the alginate with the calcium salt solution to form the hydrogel through ion cross-linking, and has a good application prospect in the detection of coliform group in water samples.

Described herein are hydrogel capsules (e.g., alginate hydrogel capsules) comprising polysaccharide polymers capable of covalent crosslinking to another moiety, such as another polysaccharide polymer, as well as related compositions and uses thereof.

This invention discloses an alginate hydrogel thin-film heat flow sensor for steel billet crystallizers, comprising an alginate hydrogel substrate with a U-shaped planar structure including a long side and a short side. A first thin-film thermocouple and a second thin-film thermocouple are respectively disposed on the inner surface of the alginate hydrogel substrate, and the first and second thin-film thermocouples are connected by cross-linking. This invention has a reasonable design, simple structure, and is easy to implement, fully utilizing the advantages of directional flow through microstructured gas film pores.

The present application relates to a kind of fecal bacteria cryopreservation and thawing method and cryoprotectant.The concentration of permeable protective agent in the fecal bacteria cryoprotectant is 350-400mg / ml, the concentration of impermeable protective agent is 110-130mg / ml, and the concentration of two-dimensional Ti3C2Tx nanosheet is 0.2-0.6mg / ml.The present application also provides a method for cryopreservation and thawing of fecal bacteria using the cryoprotectant, comprising the following steps: (1) preparing fecal bacteria; (2) preparing alginate hydrogel loaded with fecal bacteria; (3) cryopreservation of fecal bacteria; (4) thawing.The cryoprotectant of the present application contains two-dimensional Ti3C2Tx x nanosheet, so it has passive ice inhibition and photothermal active ice inhibition function.During the cryopreservation process, two-dimensional Ti3C2Tx x nanosheet has a significant inhibitory effect on the formation and growth of ice crystals.During the thawing process, two-dimensional Ti3C2Tx x nanosheet fully exerts its own photothermal effect, absorbs the energy of near-infrared laser and converts it into heat, acts as a high-efficiency space heat source, improves the uniformity of internal rewarming of the sample, and reduces the freezing damage caused by recrystallization and devitrification.